Publications by authors named "Si Wei"

186 Publications

Effect of neuromuscular block on surgical conditions during short-duration paediatric laparoscopic surgery involving a supraglottic airway.

Br J Anaesth 2021 Jun 16. Epub 2021 Jun 16.

Department of Anaesthesiology, Hunan Children's Hospital, Changsha, Hunan, China. Electronic address:

Background: Use of an LMA ProSeal™ laryngeal mask airway (P-LMA; Teleflex) with no neuromuscular block is considered a safe alternative to tracheal intubation in short-duration paediatric laparoscopic surgery. However, few studies have evaluated surgical conditions of short-duration paediatric laparoscopic surgery using this anaesthetic technique. We assessed surgical conditions for paediatric laparoscopic inguinal hernia repair using P-LMA with and without neuromuscular block.

Methods: Sixty-six patients undergoing laparoscopic inguinal hernia repair were randomised to receive a neuromuscular block (train-of-four 1-2 twitches) using rocuronium or no neuromuscular block with the P-LMA. All operations were performed by the same surgeon who determined the surgical conditions using the Leiden-surgical rating scale (L-SRS). Secondary outcomes included perioperative data, haemodynamics, and adverse events.

Results: Neuromuscular block improved surgical conditions compared with no neuromuscular block: mean (standard deviation) L-SRS 4.1 (0.5) vs 3.5 (0.6), respectively (P<0.0001). Mean rocuronium dose in the neuromuscular block group was 12.7 (4.4-29.7) mg or 0.7 (0.6-0.8) mg kg. The insufflation Ppeak was higher in the no neuromuscular block group than in the neuromuscular block group: mean (standard deviation) Ppeak 17.9 (1.8) cm HO vs 16.2 (1.9) cm HO, respectively (P=0.0004). Fifteen children (45.5%) in the no neuromuscular block group had adverse events during the surgery and anaesthesia vs four children (12.1%) in the neuromuscular block group (P=0.006).

Conclusions: Neuromuscular block significantly improved surgical conditions and reduced the incidence of adverse events during surgery and anaesthesia when an LMA Proseal™ was used in short-duration paediatric laparoscopic surgery.

Clinical Trial Registration: ChiCTR2000038529.
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http://dx.doi.org/10.1016/j.bja.2021.04.031DOI Listing
June 2021

Neuropeptide S-Mediated Modulation of Prepulse Inhibition Depends on Age, Gender, Stimulus-Timing, and Attention.

Pharmaceuticals (Basel) 2021 May 20;14(5). Epub 2021 May 20.

Institute of Physiology I, Westfälische-Wilhelms University, 48149 Münster, Germany.

Conflicting reports about the role of neuropeptide S (NPS) in animal models of psychotic-like behavior and inconsistent results from human genetic studies seeking potential associations with schizophrenia prompted us to reevaluate the effects of NPS in the prepulse inhibition (PPI) paradigm in mice. Careful examination of NPS receptor (NPSR1) knockout mice at different ages revealed that PPI deficits are only expressed in young male knockout animals (<12 weeks of age), that can be replicated in NPS precursor knockout mice and appear strain-independent, but are absent in female mice. PPI deficits can be aggravated by MK-801 and alleviated by clozapine. Importantly, treatment of wildtype mice with a centrally-active NPSR1 antagonist was able to mimic PPI deficits. PPI impairment in young male NPSR1 and NPS knockout mice may be caused by attentional deficits that are enhanced by increasing interstimulus intervals. Our data reveal a substantial NPS-dependent developmental influence on PPI performance and confirm a significant role of attentional processes for sensory-motor gating. Through its influence on attention and arousal, NPS appears to positively modulate PPI in young animals, whereas compensatory mechanisms may alleviate NPS-dependent deficits in older mice.
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http://dx.doi.org/10.3390/ph14050489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160819PMC
May 2021

[Pollution Characteristics and Risk Assessment of Nitro Polycyclic Aromatic Hydrocarbons in PM of Nanjing, China].

Huan Jing Ke Xue 2021 Jun;42(6):2626-2633

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.

Nitro polycyclic aromatic hydrocarbons (NPAHs) are important pollutants with carcinogenic effects present in PM. To analyze the pollution characteristics and sources of NPAHs, 14 samples of PM were collected in Nanjing from November 2017 to March 2018. The results showed that, 2,8-dinitrodibenzothiophene (743 pg·m), 2, 7-dinitrofluorene (331 pg·m), 9-nitroanthracene (326 pg·m), 3-nitrofluoranthene (217 pg·m), and 1,8-dinitropyrene (193 pg·m) were dominant, and the detection concentrations notably varied between seasons; the highest concentrations occurred in winter (3082 pg·m) followed by autumn (1553 pg·m) and spring (1218 pg·m). The ratio of nitrofluoranthene and 1-nitropyrene concentrations, and 9-nitroanthracene and 1-nitropyrene concentrations, indicated that the main sources of NPAHs in the PM of Nanjing were photooxidation of polycyclic aromatic hydrocarbons and biomass burning. NPAHs were more typically associated with smaller particles, which further indicated that secondary formation is an important source. The current carcinogenic risk of NPAHs in PM in Nanjing is controllable, and dinitro-polycyclic aromatic hydrocarbons represent the highest level of risk. The data presented in this study provide important baseline information that can inform the management of risks associated with NPAHs in PM in Nanjing.
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http://dx.doi.org/10.13227/j.hjkx.202009238DOI Listing
June 2021

Exposure to legacy and novel perfluoroalkyl substance disturbs the metabolic homeostasis in pregnant women and fetuses: A metabolome-wide association study.

Environ Int 2021 May 13;156:106627. Epub 2021 May 13.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.

Background: Perfluoroalkyl substances (PFASs) exist extensively and several of these have been verified to be toxic. Prenatal exposure to PFASs has attracted much attention. Metabolome-wide association analyses can be used to explore the toxicity mechanisms of PFASs by identifying associated biomarkers.

Objectives: To evaluate associations between the metabolites in maternal and cord serum and internal exposure to several common PFASs.

Methods: Paired maternal and cord serum samples were collected from 84 pregnant women who gave birth between 2015 and 2016. Seven legacy and two novel PFASs were measured. A nontarget metabolomic method and an iterative metabolite annotation based on metabolic pathways were applied to characterize the metabolic profiles. Linear regression adjusted with the false discovery rate and covariates was used to indicate the associations.

Results: A total of 279 features in maternal serum and 338 features in cord serum were identified as metabolites associated with PFAS exposure. Perfluorooctanoic acid (PFOA) and perfluorohexane sulfonic acid (PFHxS) were two PFASs associated with more metabolites, while the two novel chlorinated polyfluorinated ether sulfonic acids (Cl-PFESAs) showed less relevance to the metabolome. With pathway enrichment analysis, we found that three fatty acid metabolisms and retinol metabolism were correlated with PFAS exposure in maternal blood, and that sterol metabolism showed the correlation in both maternal serum and cord serum.

Conclusions: We identified metabolites and pathways in pregnant women and fetuses associated with the exposure to several PFAS, indicating a promising application for metabolome-wide association studies. Additional research is needed to confirm causation.
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http://dx.doi.org/10.1016/j.envint.2021.106627DOI Listing
May 2021

Unravelling molecular transformation of dissolved effluent organic matter in UV/HO, UV/persulfate, and UV/chlorine processes based on FT-ICR-MS analysis.

Water Res 2021 Jul 18;199:117158. Epub 2021 Apr 18.

State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China; Research Center for Environmental Nanotechnology (ReCENT), Nanjing University, Nanjing 210023, China. Electronic address:

Ultraviolet-based advanced oxidation processes (UV-AOPs) are very promising in advanced treatment of municipal secondary effluents. However, the transformation of dissolved effluent organic matter (dEfOM) in advanced treatment of real wastewater, particularly at molecular level, remains unclear. In this study, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) coupled with multiple statistical analysis were performed to better understand the transformation of dEfOM in UV/HO, UV/persulfate (UV/PS), and UV/chlorine treatments. An obvious increase in oxygen content of dEfOM was observed after every UV-AOPs treatment, and the detailed oxygenation processes were further uncovered by mass difference analysis based on 24 types of typical reactions. Generally, UV/HO process was subjected to the most oxygenation reactions with the typical tri-hydroxylation one (+3O), whereas di-hydroxylation reaction (+HO) was dominant in UV/PS and UV/chlorine processes. Additionally, the three UV-AOPs shared the majority of precursors, and more proportions of unique products were identified for each process. The precursors with lower H/C and higher aromaticity were readily degraded by UV/chlorine over UV/HO and UV/PS, with the products featuring lower molecular weight. Moreover, dEfOM of high aromaticity tended to produce chlorinated byproducts through addition reactions in chlorination and UV/chlorine processes. Among these UV-AOPs, the highest reduction of both acute toxicity and specific UV absorbance at 254 nm (SUVA) was observed for UV/chlorine, implying the potential for UV/chlorine process in advanced treatment of wastewater. In addition, acute toxicity was highly correlated with SUVA and CHOS compounds. This study is believed to help better understand the different fates of dEfOM in real wastewater during UV-AOPs treatment.
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http://dx.doi.org/10.1016/j.watres.2021.117158DOI Listing
July 2021

Does Subjective Dietary Knowledge Affect Sugar-Sweetened Carbonated Beverages Consumption and Child Obesity? Empirical Evidence from the Inner Mongolia Autonomous Region in China.

Int J Environ Res Public Health 2021 04 2;18(7). Epub 2021 Apr 2.

College of Economics and Management, China Agricultural University, Beijing 100083, China.

Worldwide, overweight and obesity have become an important public health problem affecting the health of children and adolescents. In China, the prevalence of overweight and obesity has reached 19 percent among the 6-17-year-old age group. Although studies have shown that regular consumption of sugar-sweetened beverages (SSBs), especially sugar-sweetened carbonated beverages (SSCBs), is positively correlated with overweight and obesity among children, the research on ways to reduce SSBs consumption is scarce. This study fills this gap by analyzing data on nearly 4000 students aged between 9-15 from the Inner Mongolia Autonomous Region in China, exploring possible influential pathways between subjective dietary knowledge, SSCBs consumption, and child obesity. The estimation results show that SSCBs consumption significantly mediates the relationship between dietary knowledge and the incidence of overweight and obesity; the mediated effects are different among subgroups. Therefore, improving dietary knowledge related to the lowing of SSBs consumption to reduce the obesity risk may be considered a possible way to reduce the prevalence of overweight and obesity among children.
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http://dx.doi.org/10.3390/ijerph18073713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037324PMC
April 2021

Complete chloroplast genome sequence of (Zygophyllaceae).

Mitochondrial DNA B Resour 2021 Apr 7;6(4):1360-1362. Epub 2021 Apr 7.

Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, PR China.

L. is a perennial herbaceous plant belonging to the family of Zygophyllaceae, and is grows in semi-arid climates, such as Xinjiang, Gansu, Ningxia, Qinghai, and Inner Mongolia in China, and also Middle East and North Africa. This species is of high medicinal value. The complete chloroplast genome was reported in this study. The chloroplast genome with a total size of 159,957 bp consists of two inverted repeats (IR, 26,550 bp) separated by a large single-copy region (LSC, 88,098 bp) and a small single-copy region (SSC, 18,759 bp). Further annotation revealed the chloroplast genome contains 113 genes, including 79 protein-coding genes, 30 genes, and four genes. A total of 90 simple sequence repeats (SSRs) were identified in the chloroplast genome. This information will be useful for study on the evolution and genetic diversity of in the future.
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http://dx.doi.org/10.1080/23802359.2021.1909441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032332PMC
April 2021

Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.

Cell 2021 Apr;184(8):2020-2032.e14

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address:

Interspecies chimera formation with human pluripotent stem cells (hPSCs) represents a necessary alternative to evaluate hPSC pluripotency in vivo and might constitute a promising strategy for various regenerative medicine applications, including the generation of organs and tissues for transplantation. Studies using mouse and pig embryos suggest that hPSCs do not robustly contribute to chimera formation in species evolutionarily distant to humans. We studied the chimeric competency of human extended pluripotent stem cells (hEPSCs) in cynomolgus monkey (Macaca fascicularis) embryos cultured ex vivo. We demonstrate that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. We also uncovered signaling events underlying interspecific crosstalk that may help shape the unique developmental trajectories of human and monkey cells within chimeric embryos. These results may help to better understand early human development and primate evolution and develop strategies to improve human chimerism in evolutionarily distant species.
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http://dx.doi.org/10.1016/j.cell.2021.03.020DOI Listing
April 2021

Response to Comment on "Suspect and Nontarget Screening of Per- and Polyfluoroalkyl Substances in Wastewater from a Fluorochemical Manufacturing Park".

Environ Sci Technol 2021 04 30;55(8):5593-5596. Epub 2021 Mar 30.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.

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http://dx.doi.org/10.1021/acs.est.1c01254DOI Listing
April 2021

GG induces cGAS/STING- dependent type I interferon and improves response to immune checkpoint blockade.

Gut 2021 Mar 8. Epub 2021 Mar 8.

Department of Radiation and Cellular Oncology, University of Chicago, Chicago, Illinois, USA

Objective: Our goals were to evaluate the antitumour efficacy of GG (LGG) in combination with immune checkpoint blockade (ICB) immunotherapies on tumour growth and to investigate the underlying mechanisms.

Design: We used murine models of colorectal cancer and melanoma to evaluate whether oral administration of LGG improves the efficacy of ICB therapies. We performed the whole genome shotgun metagenome sequencing of intestinal contents and RNA sequencing of dendritic cells (DCs). In a series of in vitro and in vivo experiments, we further defined the immunological and molecular mechanisms of LGG-mediated antitumour immunity.

Results: We demonstrate that oral administration of live LGG augmented the antitumour activity of anti-programmed cell death 1 (PD-1) immunotherapy by increasing tumour-infiltrating DCs and T cells. Moreover, the combination treatment shifted the gut microbial community towards enrichment in and , that are known to increase DC activation and CD8tumour recruitment. Mechanistically, treatment with live LGG alone or in combination with anti-PD-1 antibody triggered type I interferon (IFN) production in DCs, enhancing the cross-priming of antitumour CD8 T cells. In DCs, cyclic GMP-AMP synthase (cGAS)/stimulator of IFN genes (STING) was required for IFN-β induction in response to LGG, as evidenced by the significant decrease in IFN-β levels in cGAS or STING-deficient DCs. LGG induces IFN-β production via the cGAS/STING/TANK binding kinase 1/interferon regulatory factor 7 axis in DCs.

Conclusion: Our findings have offered valuable insight into the molecular mechanisms of live LGG-mediated antitumour immunity and establish an empirical basis for developing oral administration of live LGG as a combination agent with ICB for cancer therapies.
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http://dx.doi.org/10.1136/gutjnl-2020-323426DOI Listing
March 2021

Screening priority indicator pollutants in full-scale wastewater treatment plants by non-target analysis.

J Hazard Mater 2021 07 23;414:125490. Epub 2021 Feb 23.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 210023 Jiangsu, PR China. Electronic address:

Wastewater treatment plants (WWTPs) are the main sources of emerging contaminants (ECs) in aquatic environment. However, the standards for limiting emerging pollutants in effluent are extremely lacking. We investigated the occurrence and removal of emerging pollutants in 16 WWTPs in China using non-target analysis. 568 substances screened out were divided into 9 kinds including 167 pharmaceuticals, 113 natural substances, 85 pesticides, 86 endogenous substances, 64 chemical raw materials, 14 personal care products, 17 food additives, 6 hormones and 16 others. And they were divided into 5 fates. Pesticides and pharmaceutical compounds seemed to be the most notable categories, the kinds detected in each sample is the largest compared with other compounds. Besides, the average removal rate of pesticides and pharmaceuticals in all WWTPs were the lowest, at 9.54% and 23.77%, respectively. Priority pollutants were screened by considering distribution of pollutants with different fates. Pollutants with the same fate especially "consistent" in different WWTPs had attracted attention. 4 potential priority pollutants including metoprolol, carbamazepine, 10, 11-dihydro-10, 11-dihydroxycarbamazepine and irbesartan were proposed. And it was found that the 4 compounds, "consistent suspects" and "consistent non-targets" had similar rankings of removal rate in 16 WWTPs, which can reflect the performance of different WWTPs.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125490DOI Listing
July 2021

Photodegradation of carbon dots cause cytotoxicity.

Nat Commun 2021 02 5;12(1):812. Epub 2021 Feb 5.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu Province, China.

Carbon dots (CDs) are photoluminescent nanomaterials with wide-ranging applications. Despite their photoactivity, it remains unknown whether CDs degrade under illumination and whether such photodegradation poses any cytotoxic effects. Here, we show laboratory-synthesized CDs irradiated with light degrade into molecules that are toxic to both normal (HEK-293) and cancerous (HeLa and HepG2) human cells. Eight days of irradiation photolyzes 28.6-59.8% of the CDs to <3 kilo Dalton molecules, 1431 of which are detected by high-throughput, non-target high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Molecular network and community analysis further reveal 499 cytotoxicity-related molecules, 212 of which contain polyethylene glycol, glucose, or benzene-related structures. Photo-induced production of hydroxyl and alkyl radicals play important roles in CD degradation as affected by temperature, pH, light intensity and wavelength. Commercial CDs show similar photodegraded products and cytotoxicity profiles, demonstrating that photodegradation-induced cytotoxicity is likely common to CDs regardless of their chemical composition. Our results highlight the importance of light in cytocompatibility studies of CDs.
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http://dx.doi.org/10.1038/s41467-021-21080-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864953PMC
February 2021

Research Note: Effects of supplementing cranberry and blueberry pomaces on meat quality and antioxidative capacity in broilers.

Poult Sci 2021 Mar 9;100(3):100900. Epub 2020 Dec 9.

Department of Animal Science, McGill University, Montreal, Quebec, Canada. Electronic address:

Cranberry and blueberry pomaces are rich in antimicrobial and antioxidant compounds. They have been identified as potential antibiotic alternatives in animal feed, but their antioxidative capacity for maintaining or improving the meat quality in broilers is not well documented. This study was to determine whether cranberry and wild blueberry pomaces in diets could positively influence the broiler meat quality. A total of 3,150 broilers were randomly allotted to 10 dietary treatments with bacitracin methylene disalicylate, wild cranberry pomace (CRP) (0.5 and 1% of the basal diet), and wild blueberry pomace (BLP) (0.5 and 1% of the basal diet) alone or in combination with a mixture of feed enzymes. The results showed that supplementation with the CRP or BLP did not affect meat lightness and yellowness, while the deeper red meat (higher a∗ values) was observed in the birds receiving the diet containing 0.5% BLP against those in CRP treatments (P = 0.015). In addition, inclusion of CRP or BLP in the diet did not change meat texture and proximate composition (moisture, protein, fat, ash) irrespective of pomace concentrations. Although there were no obvious effects of CRP or BLP supplementation on meat antioxidant capacity and the incidence of myopathies (P > 0.05), the upward trend of antioxidant capacity and less severity of woody breast were observed in birds fed with 0.5% CRP. Furthermore, supplementation of 0.5 or 1.0% CRP without the enzyme resulted in higher mRNA levers of Nrf, Gpx2, and HO-1 (P < 0.05). Taken together, 0.5% CRP supplementation without the enzyme could potentially maintain meat quality and attenuate the severity of woody breast.
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http://dx.doi.org/10.1016/j.psj.2020.11.069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936115PMC
March 2021

Effects of novel probiotic strains of Bacillus pumilus and Bacillus subtilis on production, gut health, and immunity of broiler chickens raised under suboptimal conditions.

Poult Sci 2021 Mar 30;100(3):100871. Epub 2020 Nov 30.

Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, Quebec, Canada. Electronic address:

Probiotics are being developed as alternatives to antibiotic growth promoters. The aim of the study was to investigate the effects of 2 novel strains of Bacillus pumilus and Bacillus subtilis on production, intestinal microbiota, gut health, and immunity of broilers raised under suboptimal conditions. Day-old chicks (Cobb 500, n = 2,073) were randomly assigned into 6 groups: Con group (group fed with basal diet), Ab group (group treated with virginiamycin), groups treated with 2 levels of B. pumilus (low dose: 3 × 10 cfu/kg of feed [BPL] and high dose: 1 × 10 cfu/kg [BPH]), and groups treated with 2 levels of B. subtilis (low dose: 3 × 10 cfu/kg [BSL] and high dose: 1 × 10 cfu/kg [BSH]). Production parameters were recorded weekly. Cecal tonsils and content as well as ileum samples were collected on day 14 and day 42. Cecal tonsils were used to sort T-regulatory cells (CD4+CD8-CD25+ and CD4+CD8+CD25+) to study expression of IL-10 and interferon gamma, whereas cecal content was used for bacterial culture. Ileum samples were used to measure gene expression of tight junction proteins, mucin, and cytokines. BW and feed intake increased in the Ab, BPL, BSL, and BSH groups compared with the Con group between day 35 and day 42. The CD4+CD8-CD25+ cells expressed high levels of IL-10 in the BSH group on day 14 and in the BPL, BSL, and BSH groups on day 42 and high levels of interferon gamma in the BPL, BSL, and BSH groups on day 14 and in the BSL and BSH groups on day 42. The expression of IL-10 and interferon gamma in CD4+CD8+CD25+ cells was higher only in the BSH group on day 14 and day 42. Cecal bacterial populations of genera, Lactobacillus (day 14 and day 42) and Clostridium (day 14), were higher in the BSH group. Expression of tight junction protein increased significantly in the ileum on day 14 in the BPL (occludin, zona occludens 1 [ZO-1]), BSL (occludin, ZO-1), and BSH (occludin, ZO-1, junctional adhesion molecule 2 [JAM-2]) groups compared with that in the Con group and declined in all groups except in the BSH group (occludin, ZO-1, JAM-2) on day 42. Expression of MUC2 and IL-17F increased in all groups on day 14 and remained high on day 42 in the BSL and BSH groups. Taken together, both Bacillus probiotics altered the intestinal and immune activities, particularly on day 14, suggesting beneficial influence of probiotics.
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http://dx.doi.org/10.1016/j.psj.2020.11.048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936155PMC
March 2021

AT1R/GSK-3/mTOR Signaling Pathway Involved in Angiotensin II-Induced Neuronal Apoptosis after HIE Both In Vitro and In Vivo.

Oxid Med Cell Longev 2020 22;2020:8864323. Epub 2020 Dec 22.

Department of Neurosurgery, Taicang Hospital Affiliated to Soochow University, Taicang, Suzhou, Jiangsu 215400, China.

Objective: The focus of the present study is to evaluate the effects of Angiotensin II (Ang II) on neuronal apoptosis after HIE and the potential underlying mechanisms.

Methods: Primary neonatal rat cortical neurons were used to study the oxygen-glucose deprivation (OGD) cell model. The expressions of Ang II, AT1R, GSK-3, p-GSK-3, mTOR, p-mTOR, Bax, Bcl-2, and cleaved caspase-3 were detected via western blot. IF and flow cytometry were used to evaluate neuronal apoptosis. Hypoxic-ischemic encephalopathy (HIE) was established to evaluate the therapeutic effects of Ang II in vivo. Cerebral infarction areas were detected by 2,3,5-Triphenyltetrazolium chloride staining. The righting and geotaxis reflexes were also recorded. In addition, Fluoro-Jade C staining and TUNEL staining were performed to evaluate neuronal degeneration and apoptosis.

Results: Ang II significantly increased the rate of neuronal apoptosis, upregulated the expression of cleaved caspase-3, and downregulated Bcl-2/Bax ratio after OGD insult. For vivo assay, the expressions of endogenous Ang II and AT1R gradually increased and peaked at 24 h after HIE. Ang II increased NeuN-positive AT1R cell expression. In addition, Ang II increased the area of cerebral infarction, promoted neuronal degeneration and apoptosis, aggravated neurological deficits on righting and geotaxis reflexes, and was accompanied by increased expressions of phosphorylated GSK-3 and mTOR. The application of valsartan (Ang II inhibitor) or SB216763 (GSK-3 inhibitor) reversed these phenomena triggered by Ang II following HIE.

Conclusion: Ang II increased neuronal apoptosis through the AT1R/GSK-3/mTOR signaling pathway after experimental HIE both in vitro and in vivo, and Ang II may serve as a novel therapeutic target to ameliorate brain injury after HIE.
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http://dx.doi.org/10.1155/2020/8864323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773460PMC
December 2020

Target, Nontarget, and Suspect Screening and Temporal Trends of Per- and Polyfluoroalkyl Substances in Marine Mammals from the South China Sea.

Environ Sci Technol 2021 01 4;55(2):1045-1056. Epub 2021 Jan 4.

Department of Chemistry; State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Kowloon, Hong Kong SAR, China.

Per- and polyfluoroalkyl substances (PFASs) have been manufactured and widely used for over 60 years. Currently, there are thousands of marketed PFASs, but only dozens of them are routinely monitored. This work involved target, nontarget, and suspect screening of PFASs in the liver of Indo-Pacific humpback dolphin () and finless porpoise (), two resident marine mammals in the South China Sea, stranded between 2012 and 2018. Among the 21 target PFASs, perfluorooctane sulfonate and 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFESA) predominated in the samples, accounting for 46 and 30% of the total PFASs, respectively. Significantly higher total target PFAS concentrations ( < 0.05) were found in dolphin liver samples [3.23 × 10 ± 2.63 × 10 ng/g dry weight (dw)] than in porpoise liver samples (2.63 × 10 ± 1.10 × 10 ng/g dw). Significant increasing temporal trends ( < 0.05) were found in the concentrations of two emerging PFASs, perfluoroethylcyclohexane sulfonate and 2,3,3,3-tetrafluoro-2-propanoate in porpoises, indicating increasing pollution by these emerging PFASs. Forty-four PFASs from 9 classes were additionally identified by nontarget and suspect screening, among which 15 compounds were reported for the first time in marine mammals. A primary risk assessment showed that the emerging PFAS 6:2 Cl-PFESA could have possible adverse effects in terms of reproductive injury potential on most of the investigated cetaceans.
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http://dx.doi.org/10.1021/acs.est.0c06685DOI Listing
January 2021

Public health insurance and the labor market: Evidence from China's Urban Resident Basic Medical Insurance.

Authors:
Wei Si

Health Econ 2021 Feb 30;30(2):403-431. Epub 2020 Nov 30.

School of Entrepreneurship and Management, ShanghaiTech University, Shanghai, China.

This study provides empirical evidence on the labor market effects of public health insurance using evidence from China. In 2007, China launched a national public health insurance program, Urban Resident Basic Medical Insurance (URBMI), targeting residents in urban areas who were not insured by employment-based health insurance. Using panel data from the China Health and Nutrition Survey, I identify the impacts of the program based on its staggered implementation across cities. I find that URBMI did not have a significant average causal effect on labor force participation. However, it did increase employment mobility, as evidenced by the decrease in long-term employment and expansion of fixed-term contract jobs and self-employment. After the program was implemented, job lock declined and job flexibility increased, especially among women, the less educated, and individuals with good health status. The results also suggest increased employment for unhealthy workers, indicating a direct health improvement effect.
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http://dx.doi.org/10.1002/hec.4198DOI Listing
February 2021

Alcohol Use and Depression: A Mendelian Randomization Study From China.

Front Genet 2020 19;11:585351. Epub 2020 Oct 19.

College of Economics and Management, China Agricultural University, Beijing, China.

Alcohol use has been linked to a number of physical conditions, but the relationship between alcohol drinking and depression, one of the most common mental disorders that is a significant contributor to the global burden of disease, is still under debate. We aim to help fill the literature gap on the causal effect of alcohol use on depression by using genetic instruments of rs671 and rs1229984 in the Mendelian randomization (MR) framework. We collected a sample of 476 middle-aged and older adults from mainland China. The 10-item Center for Epidemiologic Studies Depression Scale (CESD-10) was used to measure the status of depression. The frequency and intensity of alcohol consumption were measured by (1) a binary indicator of drinking or not, (2) the total number of drinking occasions during the past 30 days, and (3) the weekly ethanol consumption in grams. MR estimates indicated that alcohol use was causally associated with a lower risk of depression. Parameter estimates of drinking or not ( = -0.127, = 0.048), number of drinking occasions ( = -0.012, = 0.040), and weekly ethanol consumption ( = -0.001, = 0.039) were all negative and statistically significant. The results were robust after adjustments for potential confounders (e.g., income, smoking, and parental drinking behaviors), and the exclusion of heavy or former drinkers. This is one of the first study to investigate the causal relationship between alcohol use and mental health using an MR design in East Asian populations. Further studies are needed to clarify the mechanisms of this causal link.
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http://dx.doi.org/10.3389/fgene.2020.585351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604360PMC
October 2020

Per- and Polyfluoroalkyl Substances in the Air Particles of Asia: Levels, Seasonality, and Size-Dependent Distribution.

Environ Sci Technol 2020 11 6;54(22):14182-14191. Epub 2020 Nov 6.

Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan.

Information regarding the size-dependent distribution of per- and polyfluoroalkyl substances (PFAS) in atmospheric particulate matter (PM) is very limited. In this study, 248 size-specific PM samples were collected from 9 Asian cities using a portable 4-stage cascade impactor for the analysis of PFAS. Of the 34 investigated PFAS, perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) were the major compounds. In particular, the emerging PFAS, hexafluoropropylene oxide dimer acid, was quantified in the PM for the first time, with concentrations ranging from <0.086 to 21.5 pg/m. Spatially, PFOA and PFOS were the predominant compounds in China, while precursors, emerging PFAS, and short-chain PFAS dominated in India, Japan, and South Korea, respectively. Seasonal variations of PFAS may be controlled by regional climate, local or seasonal emission sources, and long-range transport of air masses. Size-dependent distribution was investigated, showing that the majority of PFAS predominantly affiliated in fine particles, while PFOS and its alternatives tended to attach on coarser particles. Moreover, PFOS distributed on specific sizes exhibited seasonal and regional dependency, while no such patterns were observed for PFOA. These findings will provide useful information on the geographical and size-dependent distribution of PFAS in the atmospheric PM.
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http://dx.doi.org/10.1021/acs.est.0c03387DOI Listing
November 2020

A Nanoparticle-DNA Assembled Nanorobot Powered by Charge-Tunable Quad-Nanopore System.

ACS Nano 2020 11 5;14(11):15349-15360. Epub 2020 Nov 5.

School of Mechanical Engineering, Southeast University, Nanjing 211189, China.

Molecular machines hold keys to performing intrinsic functions in living cells so that the organisms can work properly, and unveiling the mechanism of functional molecule machines as well as elucidating the dynamic process of interaction with their surrounding environment is an attractive pharmaceutical target for human health. Due to the limitations of searching and exploring all possible motors in human bodies, designing and constructing functional nanorobots is vital for meeting the fast-rising demand of revealing life science and related diagnostics. Here, we theoretically designed a nanoparticle-DNA assembled nanorobot that can move along a solid-state membrane surface. The nanorobot is composed of a nanoparticle and four single-stranded DNAs. Our molecular dynamics simulations show that electroosmosis could be the main power driving the movement of a nanorobot. After the DNA strands were one-to-one captured by the nanopores in the membrane, by tuning the surface charge density of each nanopore, we have theoretically shown that the electroosmosis coupled with electrophoresis can be used to drive the movement of the nanorobot in desired directions along the graphene membrane surface. It is believed that the well-controlled nanorobot will lead to many exciting applications, such as cargo delivery, nanomanipulation, and so on, if it is implemented in the near future.
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http://dx.doi.org/10.1021/acsnano.0c05779DOI Listing
November 2020

Identification of (anti-)androgenic activities and risks of sludges from industrial and domestic wastewater treatment plants.

Environ Pollut 2021 Jan 24;268(Pt B):115716. Epub 2020 Sep 24.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China.

The annual production of sludges is significant all over the world, and large amounts of sludges have been improperly disposed by random dumping. The contaminants in these sludges may leak into the surrounding soils, surface and groundwater, or be blown into the atmosphere, thereby causing adverse effects to human health. In this study, the (anti-)androgenic activities in organic extracts of sludges produced from both industrial and domestic wastewater treatment plants (WWTPs) were examined using reporter gene assay based on MDA-kb2 cell lines and the potential (anti-)androgenic risks were assessed using hazard index (HI) based on bioassays. Twelve of the 18 samples exhibited androgen receptor (AR) antagonistic activities, with AR antagonistic equivalents ranging from 1.2 × 10 μg flutamide/g sludge to 1.8 × 10 μg flutamide/g sludge; however, no AR agonistic activity was detected in any of the tested samples. These 12 sludges were all from chemical WWTPs; no sludges from domestic WWTPs displayed AR antagonistic activity. Aside from wastewater source, treatment scale and technology could also influence AR antagonistic potencies. The HI values of all the 12 sludges exceeded 1.0, and the highest HI value was above 3.0 × 10 for children; this indicates that these sludges might cause adverse effects to human health and that children are at a greater risk than adults. The anti-androgenic potencies and risks of the subdivided fractions were also determined, and medium-polar and polar fractions were found to have relatively high detection rates and contribution rates to the AR antagonistic potencies and risks of the raw sample extracts.
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http://dx.doi.org/10.1016/j.envpol.2020.115716DOI Listing
January 2021

Correction to: Combination of multi-model statistical analysis and quantitative fingerprinting in quality evaluation of Shuang-huang-lian oral liquid.

Anal Bioanal Chem 2020 Nov 1;412(29):8223. Epub 2020 Oct 1.

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, Liaoning, China.

The authors would like to call the reader's attention to the fact that unfortunately there was a mistake in Table 1 of this contribution.
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http://dx.doi.org/10.1007/s00216-020-02937-6DOI Listing
November 2020

Combination of multi-model statistical analysis and quantitative fingerprinting in quality evaluation of Shuang-huang-lian oral liquid.

Anal Bioanal Chem 2020 Oct 18;412(26):7073-7083. Epub 2020 Aug 18.

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, Liaoning, China.

A model consisting of quantitative fingerprinting integrated with fundamental statistical analysis was established to carry out quality control analysis of Shuang-huang-lian (SHL) oral liquid. The quantitative fingerprinting approach was developed by systematic investigation of the chromatographic condition and optimization of a gradient using a complex sample analysis software system (CSASS). Five pivotal components from three traditional Chinese medicines of SHL oral liquid were determined at dual wavelengths, including phillyrin, baicalin, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid. Among them, neochlorogenic acid and cryptochlorogenic acid were quantified by quantitative analysis of multi-components with a single marker (QAMS) method. Further, the developed quantitative fingerprinting approach was validated. Good linearity with correlation coefficients (R) higher than 0.9999 were achieved for phillyrin, baicalin and chlorogenic acid. Recoveries of the three analytes were between 96% and 108%. Relative standard deviation (RSD) values were below 3% regarding the stability and intra-day and inter-day precision. Besides, the feasibility of the QAMS method was verified by an external standard method (ESM) using 18 batches of SHL oral liquid. Fifty-nine batches of SHL oral liquid from nine manufacturers were then analyzed. Effective distinction was realized based on a linear principal component analysis (linear-PCA) model by the combination of the quantitative data and chromatographic fingerprinting. The linear-PCA model based on quantitative chromatographic fingerprinting exhibited great advantage over conventional similarity analysis to distinguish between different samples. The strategy provided a particularly simple and effective approach for quality evaluation of SHL oral liquid from various manufacturers. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-02841-zDOI Listing
October 2020

Cobalt vacancies assisted ion diffusion in CoAlO carbon nanofibers for enhancing lithium battery performance.

Dalton Trans 2020 Aug 14;49(29):10127-10137. Epub 2020 Jul 14.

MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Science, Xi'an Jiaotong University, Xi'an 710049, China.

The rational design of one-dimensional nanofibers, concentrating on the compositions, morphology, structure and defects, has emerging importance in the preparation of anode materials with desired performance for lithium-ion batteries. In the present work, we prepared cobalt vacancies enriched CoAlO/carbon nanofibers coated with CoAlO nanosheets by using electrospinning and multi-step sintering processes. As the anode of the lithium-ion battery, the as-prepared nanofibers show excellent cycling stability, and particularly the discharge capacity can remain at 627.4 mA h g after 500 cycles under 500 mA g. We contributed the improved performances to the carbon-based networks, the presence of cobalt vacancy on CoAlO and the larger specific surface area of the present species. Moreover, density functional theory (DFT) calculations have implied that introducing Co vacancies could reduce the energy barrier of ion diffusion, leading to a faster diffusion rate of lithium ions during cycling. Apparently, the present approach could afford many essential advantages for anode material preparation, such as carbon-based matrix, larger specific surface area and cation vacancy, and more importantly, it can be extended to other spinel mixed transition metal oxides.
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http://dx.doi.org/10.1039/d0dt01842bDOI Listing
August 2020

Non-target and suspect screening of per- and polyfluoroalkyl substances in Chinese municipal wastewater treatment plants.

Water Res 2020 Sep 19;183:115989. Epub 2020 Jun 19.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China. Electronic address:

Wastewater treatment plant (WWTP) is one of the major sources of per- and polyfluoroalkyl substances (PFASs) to the aquatic environment. In this study, wastewater samples were collected from 17 WWTPs in 17 cities of China to investigate emerging PFASs in WWTPs. To comprehensively identify PFASs in the wastewater samples, an integrated suspect screening, homologue-based and fragment-based non-target screening method is proposed. Sixty-three PFASs from 13 classes (25 subclasses) were identified, including 14 legacy and 49 emerging PFASs, and this study is the first to report on 12 of these PFASs. We found that emerging PFASs concentration had a significantly positive correlation with the gross domestic product, indicating more substitution of legacy PFASs in the developed area of China. We also analyzed the removal of the 13 PFAS classes, and found that all discovered PFAS classes were not completely removed after the treatment process, whereas the class of perfluoroalkyl ether alcohols significantly increased. All of these results imply that the release of emerging or unknown PFASs from WWTPs is a universal but not negligible problem in China.
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http://dx.doi.org/10.1016/j.watres.2020.115989DOI Listing
September 2020

Mechanistic in silico modeling of bisphenols to predict estrogen and glucocorticoid disrupting potentials.

Sci Total Environ 2020 Aug 23;728:138854. Epub 2020 Apr 23.

State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, People's Republic of China; Jiangsu Environmental Monitoring Center, Nanjing University, Nanjing, Jiangsu 210023, People's Republic of China; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, Nanjing University, Nanjing, Jiangsu 210023, People's Republic of China.

Endocrine disrupting chemicals (EDCs) can act as agonists, antagonists or mixed agonists/antagonists toward estrogen receptor α (ERα) and glucocorticoid receptor (GR) in a tissue- and cell-specific manner. However, the activation/inhibition mechanism by which structurally different chemicals induce various types of disruption remain ambiguous. This unrevealed theory limited the in silico modeling of EDCs and the prioritization of potential EDCs for experimental testing. As a kind of chemical widely used in manufacture, bisphenols (BPs) have attracted great attentions on their potential endocrine disrupting effects. BPs used in this study exhibited pure agonistic, pure antagonistic or mixed agonistic/antagonistic activities toward ERα and/or GR. According to the mechanistic modeling, the pure agonistic and pure antagonistic activities were attributed to a single type of protein conformation induced by BPs-ERα and/or BPs-GR interactions, whereas the mixed agonistic/antagonistic activities were attributed to multiple conformations that concomitantly exist. After interacting with BPs, the active conformation recruits coactivator to induce agonistic activity and the blocked conformation inhibits coactivator to induce antagonistic activity, whereas the concomitantly-existing multiple conformations (active, blocked and competing conformations) recruit coactivator, recruit corepressor and/or inhibit coactivator to dually induce the agonistic and antagonistic activities. Therefore, the in silico modeling in this study can not only predict ERα and GR disrupting activities but also, especially, identify the potential mechanisms. This mechanistic study breaks the current bottleneck of computational toxicology and can be widely used to prioritize potential estrogen/glucocorticoid disruptor for experimental testing in both pre-clinic and clinic studies.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138854DOI Listing
August 2020

Concentration effects on capture rate and translocation configuration of nanopore-based DNA detection.

Electrophoresis 2020 09 11;41(16-17):1523-1528. Epub 2020 Jun 11.

Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, P. R. China.

Nanopore is a kind of powerful tool to detect single molecules and investigate fundamental biological processes. In biological cells or real detection systems, concentration of DNA molecules is various. Here, we report an experimental study of the effects of DNA concentration on capture rate and translocation configuration with different sized nanopores and applied voltages. Three classes of DNA translocation configurations have been observed including linear translocation, folded translocation, and cotranslocation. In the case of relatively large sized nanopore or high applied voltage, considerable cotranslocation events have been detected. The percentage of cotranslocation events also increases with DNA concentration, which leads to the relationship between capture rate and DNA concentration deviates from linearity. Therefore, in order to reflect the number of translocation molecules accurately, the capture rate should be corrected by double-counting cotranslocation events. These results will provide a valuable reference for the design of nanopore sensors.
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http://dx.doi.org/10.1002/elps.202000016DOI Listing
September 2020

differentiation of rhesus macaque bone marrow- and adipose tissue-derived MSCs into hepatocyte-like cells.

Exp Ther Med 2020 Jul 22;20(1):251-260. Epub 2020 Apr 22.

School of Medicine, Yunnan University, Kunming, Yunnan 650091, P.R. China.

Orthotopic liver or hepatocyte transplantation is effective for the treatment of acute liver injury and end-stage chronic liver disease. However, both of these therapies are hampered by the extreme shortage of organ donors. The clinical application of cell therapy through the substitution of hepatocytes with mesenchymal stem cells (MSCs) that have been differentiated into hepatocyte-like cells (HLCs) for liver disease treatment is expected to overcome this shortage. Bone marrow and adipose tissue are two major sources of MSCs [bone marrow-derived MSCs (BM-MSCs) and adipose tissue-derived MSCs (AT-MSCs), respectively]. However, knowledge about the variability in the differentiation potential between BM-MSCs and AT-MSCs is lacking. In the present study, the hepatogenic differentiation potential of rhesus macaque BM-MSCs and AT-MSCs was compared with the evaluation of morphology, immunophenotyping profiles, differentiation potential, glycogen deposition, urea secretion and hepatocyte-specific gene expression. The results indicated that BM-MSCs and AT-MSCs shared similar characteristics in terms of primary morphology, surface markers and trilineage differentiation potential (adipogenesis, osteogenesis and chondrogenesis). Subsequently, the hepatogenic differentiation potential of BM-MSCs and AT-MSCs was evaluated by morphology, glycogen accumulation, urea synthesis and expression of hepatocyte marker genes. The results indicated that rhesus BM-MSCs and AT-MSCs had hepatogenic differentiation ability. To the best of our knowledge, this is the first report to detect the hepatogenic differentiation potential of rhesus macaque BM-MSCs and AT-MSCs. The present study provides the basis for the selection of seed cells that can trans-differentiate into HLCs for cytotherapy of acute or chronic liver injuries in either clinical or veterinary practice.
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http://dx.doi.org/10.3892/etm.2020.8676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273898PMC
July 2020

Effects of cryopreservation and long-term culture on biological characteristics and proteomic profiles of human umbilical cord-derived mesenchymal stem cells.

Clin Proteomics 2020 24;17:15. Epub 2020 May 24.

Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 China.

Background: Human umbilical cord-derived MSCs (hUC-MSCs) have been identified as promising seeding cells in tissue engineering and clinical applications of regenerative medicine due to their advantages of simple acquisition procedure and the capability to come from a young tissue donor over the other MSCs sources. In clinical applications, large scale production is required and optimal cryopreservation and culture conditions are essential to autologous and allogeneic transplantation in the future. However, the influence of cryopreserved post-thaw and long-term culture on hUC-MSCs remains unknown, especially in terms of specific protein expression. Therefore, biological characteristics and proteomic profiles of hUC-MSCs after cryopreserving and long-term culturing were investigated.

Methods: Firstly, hUC-MSCs were isolated from human umbilical cord tissues and identified through morphology, surface markers and tri-lineage differentiation potential at passage 3, and then the biological characteristics and proteomic profiles were detected and compared after cryopreserving and long-term culturing at passage 4 and continuously cultured to passage 10 with detection occurring here as well. The proteomic profiles were tested by using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique and differential protein were confirmed by mass spectrometry.

Results: The results showed no significant differences in phenotypes including morphology, surface marker and tri-lineage differentiation potential but have obvious changes in translation level, which is involved in metabolism, cell cycle and other pathways.

Conclusion: This suggests that protein expression may be used as an indicator of hUC-MSCs security testing before applying in clinical settings, and it is also expected to provide the foundation or standardization guide of hUC-MSCs applications in regenerative medicine.
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http://dx.doi.org/10.1186/s12014-020-09279-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247169PMC
May 2020

Suspect screening and risk assessment of pollutants in the wastewater from a chemical industry park in China.

Environ Pollut 2020 Aug 2;263(Pt B):114493. Epub 2020 Apr 2.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210046, People's Republic of China. Electronic address:

Owing to the production and use of chemicals in chemical industry parks (CIPs), these areas are considered to be highly polluted. However, the type of pollutants presents in the wastewater from CIPs and the risk posed to the environment due to the release of these pollutants remains unclear. In this study, suspect screening was combined with traceability analysis to determine the type of pollutants present in wastewaters at 9 chemical enterprises and wastewater treatment plants (WWTPs) in the CIPs. Additionally, the distribution of nine pollutants from the WWTPs' effluent stage and the risk they posed to the surrounding river was examined through target analysis. Upon conducting suspect analysis, the presence of 65 and 64 chemicals in the 9 chemical enterprises' wastewaters and WWTPs, respectively, was tentatively identified. Traceability analysis of the compounds screened in the effluent from the WWTPs determined that 41 substances were identified as characteristic pollutants of the chemical enterprises, indicating that the suspect screening strategy enabled relatively more efficient identification of the characteristic pollutants compared to traditional quantitative analysis. Targeting analysis combined with ecological risk assessment showed that metolachlor, carbendazim, atrazine, diuron, and chlorpyrifos posed relatively higher risks to aquatic organisms in the surrounding river. Therefore, the refined management of the wastewater treatment plant in the CIPs is necessary.
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http://dx.doi.org/10.1016/j.envpol.2020.114493DOI Listing
August 2020