Publications by authors named "Si Chen"

1,018 Publications

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Synergizing metal-support interactions and spatial confinement boosts dynamics of atomic nickel for hydrogenations.

Nat Nanotechnol 2021 Jul 26. Epub 2021 Jul 26.

Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, CAS Center for Excellence in Nanoscience, University of Science and Technology of China, Hefei, China.

Atomically dispersed metal catalysts maximize atom efficiency and display unique catalytic properties compared with regular metal nanoparticles. However, achieving high reactivity while preserving high stability at appreciable loadings remains challenging. Here we solve the challenge by synergizing metal-support interactions and spatial confinement, which enables the fabrication of highly loaded atomic nickel (3.1 wt%) along with dense atomic copper grippers (8.1 wt%) on a graphitic carbon nitride support. For the semi-hydrogenation of acetylene in excess ethylene, the fabricated catalyst shows extraordinary catalytic performance in terms of activity, selectivity and stability-far superior to supported atomic nickel alone in the absence of a synergizing effect. Comprehensive characterization and theoretical calculations reveal that the active nickel site confined in two stable hydroxylated copper grippers dynamically changes by breaking the interfacial nickel-support bonds on reactant adsorption and making these bonds on product desorption. Such a dynamic effect confers high catalytic performance, providing an avenue to rationally design efficient, stable and highly loaded, yet atomically dispersed, catalysts.
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http://dx.doi.org/10.1038/s41565-021-00951-yDOI Listing
July 2021

Non--Dominated Vaginal Microbiota Is Associated With a Tubal Pregnancy in Symptomatic Chinese Women in the Early Stage of Pregnancy: A Nested Case-Control Study.

Front Cell Infect Microbiol 2021 7;11:659505. Epub 2021 Jul 7.

Department of Gynecology, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

The features of the vaginal microbiota (VM) community can reflect health status, and they could become new biomarkers for disease diagnosis. During pregnancy, domination of bacteria of the genus in the VM community is regarded as a keystone because they stabilize the VM by producing antimicrobial compounds and competing adhesion. An altered VM composition provides a marker for adverse pregnancy outcomes. This nested case-control study aimed to characterize the VM in women with a tubal pregnancy (TP) presenting with pain and/or uterine bleeding in early pregnancy. Chinese women with a symptomatic early pregnancy of unknown location were the study cohort. 16S rDNA gene-sequencing of V3-V4 variable regions was done to assess the diversity, structures, taxonomic biomarkers, and classification of the VM community. The primary outcome was the location of the early pregnancy. The VM community in women with a TP showed higher diversity (PD-whole-tree, median: 8.26 . 7.08, = 0.047; Shannon Diversity Index, median: 1.43 0.99, = 0.03) and showed different structures to those in women with an intrauterine pregnancy (IUP) ( = 0.23, < 0.01). Bacteria of the genus were significantly enriched in the IUP group, whereas bacteria of the genera and were significantly enriched in the TP group. abundance could be used to classify the pregnancy location (AUC = 0.81). Non--dominated microbiota (≤ 0.85% ) was significantly associated with a TP (adjusted odds ratio: 4.42, 95% confidence interval: 1.33 to 14.71, = 0.02). In conclusion, among women with a symptomatic early pregnancy, a higher diversity and lower abundance of in the VM is associated with a TP.
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http://dx.doi.org/10.3389/fcimb.2021.659505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294389PMC
July 2021

Zero-Valent Palladium Single-Atoms Catalysts Confined in Black Phosphorus for Efficient Semi-Hydrogenation.

Adv Mater 2021 Jul 23:e2008471. Epub 2021 Jul 23.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.

Single-atom catalysts (SACs) represent a new frontier in heterogeneous catalysis due to their remarkable catalytic properties and maximized atomic utilization. However, single atoms often bond to the support with polarized electron density and thus exhibit a high valence state, limiting their catalytic scopes in many chemical transformations. Here, it is demonstrated that 2D black phosphorus (BP) acts as giant phosphorus (P) ligand to confine a high density of single atoms (e.g., Pd , Pt ) via atomic layer deposition. Unlike other 2D materials, BP with relatively low electronegativity and buckled structure favors the strong confinement of robust zero-valent palladium SACs in the vacancy site. Metallic Pd /BP SAC shows a highly selective semi-hydrogenation of phenylacetylene toward styrene, distinct from metallic Pd nanoparticles that facilitate the formation of fully hydrogenated products. Density functional theory calculations reveal that Pd atom forms covalent-like bonding with adjacent P atoms, wherein H atoms tend to adsorb, aiding the dissociative adsorption of H . Zero-valent Pd in the confined space favors a larger energy gain for the synthesis of partially hydrogenated product over the fully hydrogenated one. This work provides a new route toward the synthesis of zero-valent SACs on BP for organic transformations.
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http://dx.doi.org/10.1002/adma.202008471DOI Listing
July 2021

The Role of Retinal Connexins Cx36 and Horizontal Cell Coupling in Emmetropization in Guinea Pigs.

Invest Ophthalmol Vis Sci 2021 Jul;62(9):27

School of Optometry and Ophthalmology, and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: The purpose of this study was to determine whether retinal gap junctions (GJs) via connexin 36 (Cx36, mediating coupling of many retinal cell types) and horizontal cell (HC-HC) coupling, are involved in emmetropization.

Methods: Guinea pigs (3 weeks old) were monocularly form deprived (FD) or raised without FD (in normal visual [NV] environment) for 2 days or 4 weeks; alternatively, they wore a -4 D lens (hyperopic defocus [HD]) or 0 D lens for 2 days or 1 week. FD and NV eyes received daily subconjunctival injections of a nonspecific GJ-uncoupling agent, 18-β-Glycyrrhetinic Acid (18-β-GA). The amounts of total Cx36 and of phosphorylated Cx36 (P-Cx36; activated state that increases cell-cell coupling), in the inner and outer plexiform layers (IPLs and OPLs), were evaluated by quantitative immunofluorescence (IF), and HC-HC coupling was evaluated by cut-loading with neurobiotin.

Results: FD per se (excluding effect of light-attenuation) increased HC-HC coupling in OPL, whereas HD did not affect it. HD for 2 days or 1 week had no significant effect on retinal content of Cx36 or P-Cx36. FD for 4 weeks decreased the total amounts of Cx36 and P-Cx36, and the P-Cx36/Cx36 ratio, in the IPL. Subconjunctival 18-β-GA induced myopia in NV eyes and increased the myopic shifts in FD eyes, while reducing the amounts of Cx36 and P-Cx36 in both the IPL and OPL.

Conclusions: These results suggest that cell-cell coupling via GJs containing Cx36 (particularly those in the IPL) plays a role in emmetropization and form deprivation myopia (FDM) in mammals. Although both FD and 18-β-GA induced myopia, they had opposite effects on HC-HC coupling. These findings suggest that HC-HC coupling in the OPL might not play a significant role in emmetropization and myopia development.
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http://dx.doi.org/10.1167/iovs.62.9.27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300059PMC
July 2021

Metabolite Profiling and Transcriptome Analysis Provide Insight into Seed Coat Color in .

Int J Mol Sci 2021 Jul 5;22(13). Epub 2021 Jul 5.

Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.

The allotetraploid species (mustard) is grown worldwide as oilseed and vegetable crops; the yellow seed-color trait is particularly important for oilseed crops. Here, to examine the factors affecting seed coat color, we performed a metabolic and transcriptomic analysis of yellow- and dark-seeded . seeds. In this study, we identified 236 compounds, including 31 phenolic acids, 47 flavonoids, 17 glucosinolates, 38 lipids, 69 other hydroxycinnamic acid compounds, and 34 novel unknown compounds. Of these, 36 compounds (especially epicatechin and its derivatives) accumulated significantly different levels during the development of yellow- and dark-seeded . . In addition, the transcript levels of , ,, and were closely associated with changes to epicatechin and its derivatives during seed development, implicating this pathway in the seed coat color determinant in . . Furthermore, we found numerous variations of sequences in the genes that may be associated with the stability of seed coat color in . , . and . , which might have undergone functional differentiation during polyploidization in the species. The results provide valuable information for understanding the accumulation of metabolites in the seed coat color of and lay a foundation for exploring the underlying mechanism.
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http://dx.doi.org/10.3390/ijms22137215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268557PMC
July 2021

Doping and facet effects synergistically mediated interfacial reaction mechanism and selectivity in photocatalytic NO abatement.

J Colloid Interface Sci 2021 Jul 9;604:624-634. Epub 2021 Jul 9.

Yangtze Delta Region Institute (Huzhou) & School of Resources and Environment, University of Electronic Science and Technology of China, Huzhou 313001, China. Electronic address:

The surface atomic coordination and arrangement largely determine photocatalytic properties. Whereas, the intrinsic impact of surface microstructures on the reaction mechanism and pathway is still unclear. Herein, via constructing N-doped BiOCO photocatalysts with diverse exposed facets, (1 1 0) and (0 0 1) facet, we testify that the pivotal roles of crystal facet and doping effect on the intermediate production and reactivity for photocatalytic nitric oxide (NO) abatement. The photoreactivity of N-doped BiOCO is documented to be higher than that of the pure samples because of the enhanced light absorption and charge transfer. Further in situ probing experiments and theoretical calculations verify that the unique adsorption patterns and activated intermediates on the (1 1 0) facet facilitate the formation of final products and inhibit the generation of toxic NO by-product in terms of thermodynamics. More importantly, we found that the selective and nonselective oxidation processes are emerged over (1 1 0) and (0 0 1) facets of BiOCO, respectively.
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http://dx.doi.org/10.1016/j.jcis.2021.07.018DOI Listing
July 2021

Crystal-structure dependent reaction pathways in photocatalytic formaldehyde mineralization on BiPO.

J Hazard Mater 2021 Jul 13;420:126633. Epub 2021 Jul 13.

School of Resources and Environment, University of Electronic Science and Technology of China, Chengdu 611731, China; Yangtze Delta Region Institute (Huzhou), University of Electronic Science and Technology of China, Huzhou 313001, China. Electronic address:

Formaldehyde as significant environmental hazard in air seriously harm the environment and human health. Although photocatalysis has demonstrated the possibility for HCHO degradation, it has long been limited by unsatisfied degradation efficiency and the unclear reaction mechanism. Here, we confirm that surface atomic arrangement of BiPO plays a critical role in photooxidation of HCHO via modulating the reaction pathway, offering 2.63 times enhancement of HCHO degradation efficiency. We dissect the processes in the photocatalytic reaction by DFT calculation, ROS monitoring, and in situ diffuse reflectance infrared Fourier transform spectra (DRIFTS) investigation. Specifically, we reveal that the controlling surface atomic arrangement could modulate adsorption model from single-point to bridging, and promote activation of small molecules. Concurrently, the active surface dependent on crystal structure facilitates the efficient transformation of intermediates (HCOOH*) (reducing energy barrier from 0.41 to -0.35 eV), producing final-product (HCO, ∆G = -0.35 eV) while suppressing toxic by-product (CO, ∆G = 0.32 eV), which contributes to the sustained deep mineralization of HCHO with enhancement by 61.4%. The findings are crucial as they provide crystal-structure related insights into the design of efficient catalysts for photocatalytic HCHO degradation. Ultimately, current molecular understanding should unlock the solar-driven catalytic pathways for other oxidation reactions.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126633DOI Listing
July 2021

Morphological changes of the anterior alveolar bone due to retraction of anterior teeth: a retrospective study.

Head Face Med 2021 Jul 16;17(1):30. Epub 2021 Jul 16.

Department of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, 22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, People's Republic of China.

Backgroud: To analyze the morphological changes of the anterior alveolar bone after the retraction of incisors in premolar extraction cases and the relationship between incisor retraction and remodeling of the alveolar base represented by points A and B displacements.

Methods: Pre- (T0) and post-treatment (T1) lateral cephalograms of 308 subjects in the maxilla and 154 subjects in the mandible who underwent the orthodontic treatment with extraction of 2 premolars in upper or lower arches were included. Alveolar bone width and height in both the maxillary and mandible incisor area were measured at T0 and T1 respectively. By superimposing the T0 and T1 cephalometric tracings, changes of points A and B, and the movement of the incisors were also measured. Then the correlation between incisor movement and the displacements of points A and B was analyzed.

Results: The alveolar bone width (ABW) showed a significant decrease in both maxilla and mandible (P < 0.001) except the labial side of the mandible (P > 0.05). The alveolar bone height (ABH) showed a significant increase in the labial side of maxilla and a significant decrease in the lingual side of maxilla and mandible. A strong positive correlation was verified between incisor movement and position changes of points A and B in both horizontal and vertical directions.

Conclusions: Anterior alveolar bone width and height generally decreased after orthodontic treatment. Incisor retraction led to significant position changes of points A and B. The decrease of anterior alveolar bone due to significant incisor retraction should be taken into account in treatment planning.
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http://dx.doi.org/10.1186/s13005-021-00277-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284009PMC
July 2021

Prediction of 1-year mortality after heart transplantation using machine learning approaches: A single-center study from China.

Int J Cardiol 2021 Jul 13. Epub 2021 Jul 13.

Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: Heart transplantation (HTx) remains the gold-standard treatment for end-stage heart failure. The aim of this study was to establish a risk-prediction model for assessing prognosis of HTx using machine-learning approach.

Methods: Consecutive recipients of orthotopic HTx at our institute between January 1st, 2015 and December 31st, 2018 were included in this study. The primary outcome was 1-year mortality. Least absolute shrinkage and selection operator method was used to select variables and seven different machine-learning approaches were employed to develop the risk-prediction model. Bootstrap method was used for model validation. Shapley Additive exPlanations (SHAP) method was used for model interpretation.

Results: 381 recipients were included with average age of 43.783 years old. Albumin, recipient age and left atrium diameter ranked top three most important variables that affected the 1-year mortality of HTx. Other important variables included red blood cell, hemoglobin, lymphocyte%, smoking history, use of lyophilized rhBNP, use of Levosimendan, hypertension, cardiac surgery history, malignancy and endotracheal intubation history. Random Forest (RF) model achieved the best area under curves (AUC) of 0.801 and gradient boosting machine (GBM) showed the best sensitivity of 0.271. SHAP method was introduced to display the RF model's predicting processes of "survival" or "death" in individual level.

Conclusions: We established the risk-prediction model for postoperative prognosis of HTx patients by using machine learning method and demonstrated that the RF model performed the highest discrimination with the largest AUC when validated. This prediction model could help to recognize high-risk HTx recipients, provide personalized therapy plan and reduce organ wastage.
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http://dx.doi.org/10.1016/j.ijcard.2021.07.024DOI Listing
July 2021

Platelet-leukocyte aggregates - a predictor for acute kidney injury after cardiac surgery.

Ren Fail 2021 Dec;43(1):1155-1162

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Background: Acute kidney injury (AKI) is one of the most common complications after cardiac surgery. However, effective biomarker used for early diagnosis of AKI has not been identified. Platelet-leukocyte aggregates (PLAs) participate in inflammation and coagulation, leading to vascular lesions and tissue destruction. We designed a prospective study to assess whether PLAs can serve as a good biomarker for early diagnosis of AKI after cardiac surgery.

Methods: Patients with rheumatic heart disease scheduled to undergo valve replacement surgery were enrolled. Blood samples were collected at five timepoints as follows: (a) At baseline. (b) At the end of extracorporeal circulation. (c) Arrival at intensive care unit (ICU). (d) Four-hours after the admission to ICU. (e) Twenty hours after the admission to ICU. After collection, the samples were immediately used for PLAs measurement by flow cytometry.

Results: A total of 244 patients were registered, and 15 of them were diagnosed with AKI according to the serum creatinine of KDIGO guidelines. The PLAs levels in AKI group were significantly increased 20 h after surgery (two-way repeated measure analysis of variance,  < 0.01) compared with that at baseline. Patients whose preoperative PLAs were higher than 6.8% showed increased risk of developing AKI (multivariate logistic regression;  = 0.01; adjusted odds ratio, 1.05; 95% confidence interval, 1.01-1.09).

Conclusion: PLAs is an independent risk factor for AKI after valve replacement among patients with rheumatic heart disease.
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http://dx.doi.org/10.1080/0886022X.2021.1948864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288121PMC
December 2021

Bee Pollen Polysaccharide From Thunb. (Rosaceae) Promotes Pancreatic β-Cell Proliferation and Insulin Secretion.

Front Pharmacol 2021 28;12:688073. Epub 2021 Jun 28.

Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China.

Insufficient pancreatic β-cell or insulin-producing β-cell are implicated in all types of diabetes mellitus. Our previous studies showed bee pollen polysaccharide RBPP-P improves insulin resistance in type 2 diabetic mice by inhibiting liver fat deposition. However, its potential of regulating β-cell function and integrity is not fully known. Herein, we observed that β-cell proliferation ( = 10), insulin synthesis ( = 5, 0.01684) and insulin incretion ( = 5, 0.02115) were intensely activated in MIN6 cells when treatment with RBPP-P. In alloxan-induced diabetic mice, oral administration of RBPP-P ( = 10) effectively decreased the blood glucose ( 0.0326), drink intake ( < 0.001) and urine ( < 0.001). It directly stimulated phosphorylation of p38 ( 0.00439), ERK ( 0.02951) and AKT ( 0.0072) to maintain the islet function and mass. Thus, our data suggest that RBPP-P is a natural compound to regulate β-cell proliferation and function, indicating it might have therapeutic potential against type 1 diabetes.
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http://dx.doi.org/10.3389/fphar.2021.688073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273306PMC
June 2021

Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker.

Research (Wash D C) 2021 19;2021:9798580. Epub 2021 Jun 19.

Center for Molecular Imaging Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Stem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1,000-1,700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100 m single-cell cluster spatial resolution, along with the lung contraction and heart beating. As such, this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.
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http://dx.doi.org/10.34133/2021/9798580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237598PMC
June 2021

Evaluation of the Lung Immune Prognostic Index in Non-Small Cell Lung Cancer Patients Treated With Systemic Therapy: A Retrospective Study and Meta-Analysis.

Front Oncol 2021 24;11:670230. Epub 2021 Jun 24.

Department of Respiratory and Critical Care Medicine, Affiliated Jinling Hospital, School of Medicine, Southeast University, Sch Med, Nanjing, China.

The lung immune prognostic index (LIPI) has been shown to be an important prognostic marker for various tumors. However, the prognostic value of LIPI among non-small cell lung cancer (NSCLC) patients treated with systemic therapy remains controversial. We aimed to evaluate survival status according to LIPI among NSCLC patients receiving different forms of systemic therapy at our institution. We also performed a meta-analysis of articles from PubMed and Embase to illustrate this question. For our cohort, we found that good LIPI was associated with better overall survival (OS) among 91 patients on immunotherapy, 329 patients on targeted therapy, and 570 patients on chemotherapy. For the meta-analysis, a total of eight studies with 8,721 patients were included. Pooled results showed that a higher LIPI (those with 1 or 2 factors) was associated with poor overall progression-free survival (PFS) (hazard ratio [HR], 1.57; 95% confidence interval [CI], 1.45-1.71) and OS (HR, 2.01; 95% CI, 1.75-2.31). Subgroup analyses showed that a higher LIPI was related to poor survival among patients prescribed different systemic therapies: immunotherapy (OS HR, 2.50; 95% CI, 1.99-3.13; PFS HR, 1.77; 95% CI, 1.56-2.01), chemotherapy (OS HR, 1.58; 95% CI, 1.34-1.86; PFS HR, 1.38; 95% CI, 1.23-1.55), and targeted therapy (OS HR; 2.15, 95% CI, 1.57-2.96; PFS HR, 1.60; 95% CI, 1.25-2.06). The study shows that the LIPI is a clinically significant prognostic factor for NSCLC patients receiving systemic therapy.

Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, identifier CRD420209009.
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http://dx.doi.org/10.3389/fonc.2021.670230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264771PMC
June 2021

Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to strain M5-90.

PeerJ 2021 29;9:e11679. Epub 2021 Jun 29.

Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Hainan Key Lab of Tropical Animal Reproduction, Haikou, Hainan, China.

Brucellosis is a globally zoonotic bacterial disease of humans and various animals including goats, sheep, and cattle. M5-90, a live attenuated vaccine strain, has been widely used to prevent brucellosis in goats and sheep. However, the molecular mechanisms governing protective immunity response in non-professional phagocytes infected with M5-90 have not been fully investigated, especially in goats. In our research, goat fibroblasts were used as in vitro models to determine these mechanisms by transcriptome analysis. After incubating with M5-90 3 h, the infected goat fibroblasts were collected at 0 h, 4 h, 24 h, 48 h and 72 h for RNA-seq. The results indicated that there were totally 11,819 differentially expressed genes (DEGs) and 777 differentially expressed (DE) miRNAs found in experiment groups compared with the control groups (|log2(Foldchange)|≥1, FDR<0.05). GO and KEGG enrichment analyses revealed that down-regulated genes were involved in the riboflavin metabolism and positive regulation of IL-8 secretion pathway. The up-regulated genes were mainly involved in adaptive immunity, including TNF signaling pathway, MAPK signaling pathway and JAK/STAT pathway. Additionally, cytokine-cytokine receptor interaction, natural killer cell mediated cytotoxicity and toll-like receptor signaling pathway, which associated with innate immunity pathways, were also induced. Based on the Pearson correlation coefficients and prediction results of TargetScan and miRanda, the miRNA-mRNA networks of , and were constructed and verified in goat fibroblasts by qPCR, which demonstrated that goat fibroblasts displayed immunomodulatory properties. Our findings provide a deeper insight into the host miRNA-driven defense mechanism and reveal the transcriptome changes involved in the innate and adaptive immune response of the goats to infection.
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http://dx.doi.org/10.7717/peerj.11679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253117PMC
June 2021

Organ-Specific Transcriptome Analysis Identifies Candidate Genes Involved in the Stem Specialization of Bermudagrass ( L.).

Front Genet 2021 23;12:678673. Epub 2021 Jun 23.

College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

As an important warm-season turfgrass and forage grass species with wide applications, bermudagrass ( L.) simultaneously has shoot, stolon and rhizome, three types of stems with different physiological functions. To better understand how the three types of stems differentiate and specialize, we generated an organ-specific transcriptome dataset of bermudagrass encompassing 114,169 unigenes, among which 100,878 and 65,901 could be assigned to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) terms, respectively. Using the dataset, we comprehensively analyzed the gene expression of different organs, especially the shoot, stolon and rhizome. The results indicated that six organs of bermudagrass all contained more than 52,000 significantly expressed unigenes, however, only 3,028 unigenes were enrich-expressed in different organs. Paired comparison analyses further indicated that 11,762 unigenes were differentially expressed in the three types of stems. Gene enrichment analysis revealed that 39 KEGG pathways were enriched with the differentially expressed unigenes (DEGs). Specifically, 401 DEGs were involved in plant hormone signal transduction, whereas 1,978 DEGs were transcription factors involved in gene expression regulation. Furthermore, in agreement with the starch content and starch synthase assay results, DEGs encoding starch synthesis-related enzymes all showed the highest expression level in the rhizome. These results not only provided new insights into the specialization of stems in bermudagrass but also made solid foundation for future gene functional studies in this important grass species and other stoloniferous/rhizomatous plants.
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http://dx.doi.org/10.3389/fgene.2021.678673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260954PMC
June 2021

Investigation of Tactile Perception Evoked by Ridged Texture Using ERP and Non-linear Methods.

Front Neurosci 2021 24;15:676837. Epub 2021 Jun 24.

Xuzhou Central Hospital, Xuzhou, China.

The triangular ridged surface can improve the grip reliability of products, but the sharp edge of triangular ridge induces sharp and uncomfortable feeling. To study the effect of edge shape (sharp, round, and flat shape) of triangular ridges on brain activity during touching, electroencephalograph (EEG) signals during tactile perception were evaluated using event-related potentials (ERP) and non-linear analysis methods. The results showed that the early component of P100 and P200, and the late component of P300 were successfully induced during perceiving the ridged texture. The edge shape features affect the electrical activity of brain during the tactile perceptions. The sharp shape feature evoked fast P100 latency and high P100 amplitude. The flat texture with complex (sharp and flat) shape feature evoked fast P200 latency, high P200 amplitude and RQA parameters. Both of the sharp shape and complex shape feature tended to evoke high peak amplitude of P300. The large-scale structures of recurrence plots (RPs) and recurrence quantification analysis (RQA) parameters can visually and quantitatively characterize the evolution regulation of the dynamic behavior of EEG system along with the tactile process. This study proved that RPs and RQA were protential methods for the feature extraction and state recognition of EEG during tactile perception of textured surface. This research contributes to optimize surface tactile characteristics on products, especially effective surface textures design for good grip.
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http://dx.doi.org/10.3389/fnins.2021.676837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264067PMC
June 2021

ORMDL1 is upregulated and associated with favorable outcomes in colorectal cancer.

Transl Oncol 2021 Jul 6;14(10):101171. Epub 2021 Jul 6.

Guangdong Institute of Gastroenterology, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China; Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China. Electronic address:

Background: The ORMDL1 gene is known as a crucial negative regulator of sphingolipid biogenesis. However, the ORMDL1 gene has rarely been studied in a tumor-related context. Therefore, its prognostic value and functional significance in colorectal cancer (CRC) remain to be explored.

Methods: TCGA CRC cohort analysis, qRT-PCR, and immunohistochemistry (IHC) were used to examine the ORMDL1 expression level. The association between ORMDL1 expression and various clinical characteristics was analyzed by chi-square tests. The overall survival (OS) of CRC patients was analyzed by Kaplan-Meier analysis. In vitro and in vivo cell-based assays were performed to explore the role of ORMDL1 in cell proliferation, invasion and migration. Transcriptional changes in cells with either ORMDL1 knockdown or overexpression were compared and analyzed.

Results: ORMDL1 was upregulated in CRC tissues in both the TCGA and our cohort. Interestingly, its expression was significantly lower in patients with metastasis than in patients without metastasis, and the high expression group had longer OS than the low expression group. Knockdown of ORMDL1 expression can promote proliferation, colony formation and invasion, while attenuating migration in CRC cell lines. In contrast, forced overexpression of ORMDL1 reduced cell proliferation, colony formation and invasion, while enhancing cell migration. Stable knockdown of ORMDL1 can promote cancer cell proliferation in vivo to some extent. Finally, Rho GTPase activity was influenced by ORMDL1, and the expression of ORMDL1 was enhanced by DTT treatment.

Conclusion: ORMDL1 is upregulated and may serve as a biomarker to predict favourable outcomes in colorectal cancer.
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http://dx.doi.org/10.1016/j.tranon.2021.101171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273222PMC
July 2021

Therapeutic effects of quinine in a mouse model of atopic dermatitis.

Mol Med Rep 2021 May 9;23(5). Epub 2021 Jul 9.

Department of Dermatology, Shenzhen University General Hospital, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.

Atopic dermatitis (AD) is a chronic inflammatory skin disease that seriously affects quality of life. Quinine is a bitter taste receptor agonist that exhibits antimalarial effects. The aim of the present study was to examine the therapeutic effects of quinine in AD‑like mice. AD was induced with 2,4‑dinitrochlorobenzene, and the mice were treated with 10 mg/kg quinine for 1, 4 and 7 days. A total of 60 BALB/c mice were divided into the following groups: Healthy, AD‑like, AD‑like + quinine and healthy + quinine, with 1, 4 and 7 days groups for each treatment. Blood was extracted from all mice and ELISA was performed to detect immunoglobulin E (IgE) levels. H&E‑stained tissue sections were prepared from skin lesions on the backs of the mice and pathological changes were observed. Cytokines were detected via ELISA, and the filaggrin (FLG) and kallikrein‑7 (KLK7) proteins were detected via western blotting and immunohistochemistry. IKKα and NF‑κB mRNA were analyzed via reverse transcription‑quantitative PCR. Quinine ameliorated skin damage in the AD‑like mice, reduced IgE expression in the blood, inhibited expression of IKKα and NF‑κB, reduced cytokine secretion, reduced KLK7 expression, reduced scratching frequency, increased FLG expression and repaired the skin barrier. These results suggested that quinine exhibited therapeutic effects in AD‑like mice.
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http://dx.doi.org/10.3892/mmr.2021.11952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974254PMC
May 2021

Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated With Immune Reaction to in Goats.

Front Vet Sci 2021 21;8:615405. Epub 2021 Jun 21.

Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, College of Animal Science and Technology, Hainan University, Haikou, China.

is a highly versatile pathogen that infects a wide range of animals, including goats, causing pneumonia and hemorrhagic septicemia. Circular RNA (circRNA) is a type of non-coding RNA that plays an important role in regulating cellular metabolism. However, whether and how circRNA is involved in regulating immune responses in the goat lung has not been reported. Thus, this study was designed to examine the function of circRNA in goats infected with . Goats were assigned into one of two groups: an uninfected control group (CK) and an infected group challenged with . Compared with the CK group, which remained healthy, the infected goats showed clinical signs of infection, including depression, cough, nasal discharge, and dyspnea, along with elevated body temperature and lesions in the lung. Whole-transcriptome sequencing and small RNA sequencing were then performed using lung samples from goats from each group. A total of 138 circRNA, 56 microRNAs (miRNA), and 2,673 messenger RNA (mRNA) molecules were significantly differentially expressed in the -infected group compared with the CK group. Randomly selected differentially expressed circRNA, miRNA, and mRNA molecules ( = 5 per group) were then validated by quantitative reverse-transcriptase polymerase chain reaction analysis. Gene ontology (GO) analysis of the source genes indicated that six immune-related terms were enriched among the differentially expressed cirRNA molecules, including inflammatory response, immune effector process, cell activation involved in immune response, cytokine-mediated signaling pathway, response to endogenous stimulus, and immune response. The corresponding circRNA molecules were then selected for construction of a competitive endogenous RNA network to identify networks that may be involved in the immune response to infection. The results indicated that HN01 may cause pneumonia and stimulate an immune response in goats via regulation of circRNA expression. This study presents the first comprehensive circRNA profile in response to infection in goats, thus, providing a basis for understanding the function of circRNA in the host immune response to infection.
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http://dx.doi.org/10.3389/fvets.2021.615405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256745PMC
June 2021

Causing Neonatal Meningitis During 2001-2020: A Study in Eastern China.

Int J Gen Med 2021 29;14:3007-3016. Epub 2021 Jun 29.

Department of Neonatology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People's Republic of China.

Background And Objective: Neonatal meningitis (NM) caused by remains a major health problem in industrialized countries. Currently, information on the epidemiology and antimicrobial susceptibility patterns of NM in developing countries such as China is relatively scarce. Therefore, the present study investigated changes in the antimicrobial susceptibility of causing NM in a perinatal center in eastern China over the past 20 years.

Methods: This survey was conducted during three periods: 2001-2006, 2007-2012, and 2013-2020. NM was diagnosed according to the number of white blood cells in the cerebrospinal fluid (CSF) and the presence of a single potential pathogenic bacterium in the culture prepared from the blood or CSF of a newborn baby. Changes in the antimicrobial susceptibility of were analyzed.

Results: In total, 182 NM cases were identified. was identified in 69 of these cases, and in 21 of these cases, extended-spectrum beta-lactamase (ESBL) production was detected. was the main cause of NM identified in this study. The overall susceptibility of to third-generation cephalosporins such as cefotaxime decreased from 100% during 2001-2006 to 50% during 2007-2012 and, subsequently, increased to 71.0% during 2013-2020. This pattern of change is correlated with bacterial ESBL production. Only 8.3% of found in samples collected from infants with early onset meningitis (EOM) produced ESBL, while 37.3% of isolated from children with late-onset meningitis (LOM) produced ESBL.

Conclusion: remains the primary pathogen of NM. Compared with that isolated from infants with LOM, the percentage of ESBL-producing multidrug-resistant isolated from infants with EOM is significantly lower. Clinicians should consider this trend when determining appropriate and effective antibiotics as empirical treatment for NM.
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http://dx.doi.org/10.2147/IJGM.S317299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254664PMC
June 2021

Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed.

Rice (N Y) 2021 Jul 5;14(1):61. Epub 2021 Jul 5.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

Background: Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the mechanisms underlying glutelin synthesis and accumulation in tetraploid rice is of great significance.

Results: To enhance the nutritional value of rice, we developed tetraploid rice and evaluated the contents of various nutrient elements in mature seeds. The results revealed a significant increase in protein contents, including the total seed storage proteins, glutelins, and amino acids in tetraploid rice when compared with those in diploid rice. Tandem mass tag-based quantitative proteomic analyses of seeds revealed that glutelins regulated by several glutelin genes in 9311-4x were significantly up-regulated (≥1.5-fold), which was further verified by immunoblot analyses. In addition, temporal expression patterns of various glutelin subunits in different rice lines were investigated. The results revealed significant differences in the expression patterns between diploid and tetraploid rice seeds. Cytohistological analyses results revealed that the thickness of aleurone cell layers increased significantly by 32% in tetraploid rice, the structures of protein storage vacuoles (PSVs) in sub-aleurone cells were more diverse and abundant than those of diploid rice. Temporal expression and proteomic analyses results revealed that protein disulfide isomerase-like 1-1 expression levels were higher in tetraploid rice than in diploid rice, and that the gene responded to oxidative folding with increased levels of proglutelin and appropriate distribution of seed glutelins in tetraploid rice.

Conclusion: The results of the present study revealed that polyploidization increased glutelin content by influencing glutelin biosynthesis, transport, and deposition, while variations in glutelin accumulation between tetraploid and diploid rice were largely manifested in the initial time, duration, and relative levels of various glutelin gene expressions during seed filling stages. These findings provide novel insights into improving the protein quality and nutritional value of rice seeds by polyploid breeding.
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http://dx.doi.org/10.1186/s12284-021-00500-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257881PMC
July 2021

[Progress on the metabolic rules and detection methods for okadaic acid related toxins in biological samples].

Se Pu 2020 Jun;38(6):621-626

Laboratory of Aquatic Product Processing and Quality Safety, Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316021, China.

In recent years, red tide pollution in China has become increasingly serious, leading to a number of shellfish poisoning cases, thereby posing a threat to human health and safety. Okadaic acid (OA) and its analogs (dinophysistoxins, DTXs) are the most widely distributed diarrhetic shellfish poisons, which result in acute diarrheal toxicity and various types of chronic toxicity. It is imperative to establish a method for determination of OA related toxin residues in biological fluid samples, so that accurate diagnosis of poisoning in patients is possible. The present paper briefly introduced the main physicochemical properties, poisoning incidents, toxicological effects for the toxins, also summarized recent progress on the metabolic rules and detection methods for OA related toxins in biological samples.
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http://dx.doi.org/10.3724/SP.J.1123.2019.10016DOI Listing
June 2020

Redox Buffering Effects in Potentiometric Detection of DNA Using Thiol-Modified Gold Electrodes.

ACS Sens 2021 07 29;6(7):2546-2552. Epub 2021 Jun 29.

Division of Solid-State Electronics, Department of Electrical Engineering, Ångström Laboratory, Uppsala University, P.O. Box 65, Uppsala SE-75103, Sweden.

Label-free potentiometric detection of DNA molecules using a field-effect transistor (FET) with a gold gate offers an electrical sensing platform for rapid, straightforward, and inexpensive analyses of nucleic acid samples. To induce DNA hybridization on the FET sensor surface to enable potentiometric detection, probe DNA that is complementary to the target DNA has to be immobilized on the FET gate surface. A common method for probe DNA functionalization is based on thiol-gold chemistry, immobilizing thiol-modified probe DNA on a gold gate with thiol-gold bonds. A self-assembled monolayer (SAM), based on the same thiol-gold chemistry, is also needed to passivate the rest of the gold gate surface to prevent non-specific adsorption and to enable favorable steric configuration of the probe DNA. Herein, the applicability of such FET-based potentiometric DNA sensing was carefully investigated, using a silicon nanoribbon FET with a gold-sensing gate modified with thiol-gold chemistry. We discover that the potential of the gold-sensing electrode is determined by the mixed potential of the gold-thiol and gold-oxygen redox interactions. This mixed potential gives rise to a redox buffer effect which buffers the change in the surface charge induced by the DNA hybridization, thus suppressing the potentiometric signal. Analogous redox buffer effects may also be present for other types of potentiometric detections of biomarkers based on thiol-gold chemistry.
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http://dx.doi.org/10.1021/acssensors.0c02700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314270PMC
July 2021

Deep Brain Stimulation Complications in Patients With Parkinson's Disease and Surgical Modifications: A Single-Center Retrospective Analysis.

Front Hum Neurosci 2021 11;15:684895. Epub 2021 Jun 11.

Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, China.

Background: As a complication-prone operation, deep brain stimulation (DBS) has become the first-line surgical approach for patients with advanced Parkinson's disease (PD). This study aimed to evaluate the incidence and risk factors of DBS-associated complications.

Methods: We have reviewed a consecutive series of patients with PD undergoing DBS procedures to describe the type, severity, management, and outcome of postoperative complications from January 2011 to December 2018. Both univariate and multivariate analyses were performed to identify statistically significant risk factors. We also described our surgical strategies to minimize the adverse events.

Results: A total of 225 patients underwent 229 DBS implantation procedures (440 electrodes), of whom 20 patients experienced 23 DBS-associated complications, including ten operation-related complications and 13 hardware-related ones. Univariate analysis elucidated that comorbid medical conditions ( = 0.024), hypertension ( = 0.003), early-stage operation ( < 0.001), and unilateral electrode implantation ( = 0.029) as risk factors for overall complications, or more specifically, operation-related complications demonstrated in the stratified analysis. In contrast, no risk factor for hardware-related complications was identified. Statistical significances of hypertension (OR = 3.33, 95% CI: 1.14-9.71, = 0.027) and early-stage (OR = 11.04, 95% CI: 2.42-50.45, = 0.002) were further validated via multivariate analysis. As the annual number of DBS procedures increased, the incidence of complications gradually decreased ( = -0.699, < 0.01). Additionally, there was a strong correlation between surgical complications and unplanned readmission ( = 0.730, < 0.01).

Conclusion: The importance of cumulative experience and relevant technique modifications should be addressed to prevent DBS-associated complications and unplanned readmission.
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http://dx.doi.org/10.3389/fnhum.2021.684895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226223PMC
June 2021

The impact of off-balance-sheet regulations on bank risk-taking: Evidence from China.

Res Int Bus Finance 2020 Dec 17;54:101297. Epub 2020 Jul 17.

Schoolof Finance, Tianjin University of Finance and Economics, Tianjin City, PR China.

The release of new asset management rules has played a positive role in regulating the asset management business of financial institutions, preventing and controlling financial risk. It has also had an important impact on the management of banks' off-balance-sheet (OBS) innovation. This paper uses unbalanced panel data on 75 commercial banks in China from 2007 to 2017 and combines a theoretical and an empirical model to study the development of bank OBS innovation and bank risk taking from the perspective of new asset management regulations. The analysis finds (1) the rapid development of OBS innovation will increase bank risk taking and (2) the solution to the problem of rigid payment is conducive to reducing the risk taken by Chinese commercial banks when providing OBS innovation.
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http://dx.doi.org/10.1016/j.ribaf.2020.101297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366963PMC
December 2020

Pregnancy Induces an Immunological Memory Characterized by Maternal Immune Alterations Through Specific Genes Methylation.

Front Immunol 2021 7;12:686676. Epub 2021 Jun 7.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

During pregnancy, the maternal immune system undergoes major adaptive modifications that are necessary for the acceptance and protection of the fetus. It has been postulated that these modifications are temporary and limited to the time of pregnancy. Growing evidence suggests that pregnancy has a long-term impact on maternal health, especially among women with pregnancy complications, such as preeclampsia (PE). In addition, the presence of multiple immunological-associated changes in women that remain long after delivery has been reported. To explain these long-term modifications, we hypothesized that pregnancy induces long-term immunological memory with effects on maternal well-being. To test this hypothesis, we evaluated the immunological phenotype of circulating immune cells in women at least 1 year after a normal pregnancy and after pregnancy complicated by PE. Using multiparameter flow cytometry (FCM) and whole-genome bisulfite sequencing (WGBS), we demonstrate that pregnancy has a long-term effect on the maternal immune cell populations and that this effect differs between normal pregnancy and pregnancy complicated by PE; furthermore, these modifications are due to changes in the maternal methylation status of genes that are associated with T cell and NK cell differentiation and function. We propose the existence of an "immunological memory of pregnancy (IMOP)" as an evolutionary advantage for the success of future pregnancies and the proper adaptation to the microchimeric status established during pregnancy. Our findings demonstrate that the type of immune cell populations modified during pregnancy may have an impact on subsequent pregnancy and future maternal health.
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http://dx.doi.org/10.3389/fimmu.2021.686676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215664PMC
June 2021

Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.

Infect Genet Evol 2021 Jun 17;93:104971. Epub 2021 Jun 17.

College of Animal Sciences, Key Lab for Zoonoses Research, Ministry of Education, Jilin University, 5333 Xi' An Road, Changchun 130062, China. Electronic address:

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection has caused a global pandemic in the past year, which poses continuing threat to human beings. To date, more than 3561 mutations in the viral spike protein were identified, including 2434 mutations that cause amino acid changes with 343 amino acids located in the viral receptor-binding domain (RBD). Among these mutations, the most representative ones are substitution mutations such as D614G, N501Y, Y453F, N439K/R, P681H, K417N/T, and E484K, and deletion mutations of ΔH69/V70 and Δ242-244, which confer the virus with enhanced infectivity, transmissibility, and resistance to neutralization. In this review, we discussed the recent findings of SARS-CoV-2 for highlighting mutations and variants on virus transmissibility and pathogenicity. Moreover, several suggestions for prevention and controlling the pandemic are also proposed.
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http://dx.doi.org/10.1016/j.meegid.2021.104971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213438PMC
June 2021

Erythropoiesis changes with increasing age in the elderly Chinese.

Int J Lab Hematol 2021 Jun 14. Epub 2021 Jun 14.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Introduction: Erythropoiesis slowly decreases with increasing age, which may be reflected in red blood cell (RBC) parameters. This multicentre collaborative study aimed to investigate the changes in erythropoiesis with increasing age in a healthy Chinese population.

Methods: A total of 14,591 healthy individuals (6,713 aged at least 60 y and 7,878 aged below 60 y) from seven cities across China were enrolled. K2-EDTA anticoagulant blood samples were analysed. The results are presented as median and 2.5-97.5th percentile.

Results: RBC parameters showed some differences between the two groups divided by the age of 60 in the Chinese population. The median, 2.5th and 97.5th percentile values of RBC, haemoglobin (HGB) and haematocrit (HCT) in patients aged ≥ 60 y were significantly lower than in those ˂ 60 y. The values of mean cell volume (MCV), mean cell haemoglobin (MCH) and red cell distribution width (RDW) were higher in the group aged ≥ 60 y. Men had significantly higher RBC, HGB, HCT, MCV, MCH and RDW indices than women. The prevalence of anaemia gradually increased with age in men and was higher than that in women after 50. The median haemoglobin and MCV in Nanning and Guangzhou were lower than those in other regions.

Conclusion: RBC parameters varied with increasing age and differed between males and females, indicating that erythropoiesis decreases in the elderly Chinese population. Subsequent studies should be conducted for age- and sex-specific reference intervals in healthy elderly Chinese populations.
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http://dx.doi.org/10.1111/ijlh.13615DOI Listing
June 2021

Simultaneous structural and elemental nano-imaging of human brain tissue.

Chem Sci 2020 Aug 10;11(33):8919-8927. Epub 2020 Aug 10.

Brain and Mind Centre and Discipline of Pharmacology, The University of Sydney Camperdown NSW 2050 Australia

Examining chemical and structural characteristics of micro-features in complex tissue matrices is essential for understanding biological systems. Advances in multimodal chemical and structural imaging using synchrotron radiation have overcome many issues in correlative imaging, enabling the characterization of distinct microfeatures at nanoscale resolution in tissues. We present a nanoscale imaging method that pairs X-ray ptychography and X-ray fluorescence microscopy (XFM) to simultaneously examine structural features and quantify elemental content of microfeatures in complex tissues. We examined the neuropathological microfeatures Lewy bodies, aggregations of superoxide dismutase 1 (SOD1) and neuromelanin in human post-mortem Parkinson's disease tissue. Although biometals play essential roles in normal neuronal biochemistry, their dyshomeostasis is implicated in Parkinson's disease aetiology. Here we show that Lewy bodies and SOD1 aggregates have distinct elemental fingerprints yet are similar in structure, whilst neuromelanin exhibits different elemental composition and a distinct, disordered structure. The unique approach we describe is applicable to the structural and chemical characterization of a wide range of complex biological tissues at previously unprecedented levels of detail.
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http://dx.doi.org/10.1039/d0sc02844dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163372PMC
August 2020

Ultrasound-guided nusinersen administration for spinal muscular atrophy patients with severe scoliosis: an observational study.

Orphanet J Rare Dis 2021 06 13;16(1):274. Epub 2021 Jun 13.

Cardiology Department, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College, Shuaifuyuan 1#, Beijing, 100730, China.

Background: This observational study describes our experience delivering nusinersen through lumbar puncture with real-time ultrasound guidance in spinal muscular atrophy (SMA) patients with severe scoliosis.

Results: Intrathecal nusinersen via real-time ultrasound-guided lumbar puncture was given to three patients who had severe thoracic and lumbar scoliosis: a 34-year-old female with type 3a SMA, a 28-year-old male with type 2a SMA, and a 14-year-old girl with type 3a SMA. Lumbar puncture was performed without sedation under ultrasound guidance using a 22G echogenic needle in the interlaminar aspect of the L4-L5 or L5-S1 interspace and a full dose of nusinersen (12 mg/5 mL) was injected after visualizing free cerebrospinal fluid flow. Patients completed their four loading doses and one maintenance dose of nusinersen. All 15 procedures were successful and well tolerated.

Conclusions: Real-time ultrasound-guided lumbar puncture is an effective and radiation-free technique to administer intrathecal nusinersen in SMA patients with severe scoliosis when done by practitioners with expertise in this procedure.
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http://dx.doi.org/10.1186/s13023-021-01903-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201867PMC
June 2021
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