Publications by authors named "Shuyu Wang"

107 Publications

A Compact High-Quality Image Demosaicking Neural Network for Edge-Computing Devices.

Sensors (Basel) 2021 May 8;21(9). Epub 2021 May 8.

State Key Laboratory of Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Image demosaicking has been an essential and challenging problem among the most crucial steps of image processing behind image sensors. Due to the rapid development of intelligent processors based on deep learning, several demosaicking methods based on a convolutional neural network (CNN) have been proposed. However, it is difficult for their networks to run in real-time on edge computing devices with a large number of model parameters. This paper presents a compact demosaicking neural network based on the UNet++ structure. The network inserts densely connected layer blocks and adopts Gaussian smoothing layers instead of down-sampling operations before the backbone network. The densely connected blocks can extract mosaic image features efficiently by utilizing the correlation between feature maps. Furthermore, the block adopts depthwise separable convolutions to reduce the model parameters; the Gaussian smoothing layer can expand the receptive fields without down-sampling image size and discarding image information. The size constraints on the input and output images can also be relaxed, and the quality of demosaicked images is improved. Experiment results show that the proposed network can improve the running speed by 42% compared with the fastest CNN-based method and achieve comparable reconstruction quality as it on four mainstream datasets. Besides, when we carry out the inference processing on the demosaicked images on typical deep CNN networks, Mobilenet v1 and SSD, the accuracy can also achieve 85.83% (top 5) and 75.44% (mAP), which performs comparably to the existing methods. The proposed network has the highest computing efficiency and lowest parameter number through all methods, demonstrating that it is well suitable for applications on modern edge computing devices.
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http://dx.doi.org/10.3390/s21093265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125912PMC
May 2021

AI powered electrochemical multi-component detection of insulin and glucose in serum.

Biosens Bioelectron 2021 May 1;186:113291. Epub 2021 May 1.

State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110000, China. Electronic address:

Multi-component detection of insulin and glucose in serum is of great importance and urgently needed in clinical diagnosis and treatment due to its economy and practicability. However, insulin and glucose can hardly be determined by traditional electrochemical detection methods. Their mixed oxidation currents and rare involvement in the reaction process make it difficult to decouple them. In this study, AI algorithms are introduced to power the electrochemical method to conquer this problem. First, the current curves of insulin, glucose, and their mixed solution are obtained using cyclic voltammetry. Then, seven features of the cyclic voltammetry curve are extracted as characteristic values for detecting the concentrations of insulin and glucose. Finally, after training using machine learning algorithms, insulin and glucose concentrations are decoupled and regressed accurately. The entire detection process only takes three minutes. It can detect insulin at the pmol level and glucose at the mmol level, which meets the basic clinical requirements. The average relative error in predicting insulin concentrations is around 6.515%, and that in predicting glucose concentrations is around 4.36%. To verify the performance and effectiveness of the proposed method, it is used to determine the concentrations of insulin and glucose in fetal bovine serum and real clinical serum samples. The results are satisfactory, demonstrating that the method can meet basic clinical needs. This multi-component testing system delivers acceptable detect limit and accuracy and has the merits of low cost and high efficiency, holding great potential for use in clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2021.113291DOI Listing
May 2021

The effects of multiple controlled ovarian hyperstimulation over a 2-year period on ovarian reserve and reactivity: a retrospective clinical study.

Ann Palliat Med 2021 Apr;10(4):4391-4397

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Background: To evaluate the effects of controlled ovarian hyperstimulation (COH) on ovarian reserve function during in vitro fertilization and embryo transfer (IVF-ET).

Methods: From August 2018 to August 2020, the medical records of patients who received IVF-ET in the Department of Reproductive Medicine, Beijing Gynaecology and Obstetrics Hospital, Capital Medical University were analyzed retrospectively. Among them, 372 patients received 2 cycles of COH, 54 patients received 3 cycles, and 13 patients received 4 cycles. The levels of follicle-stimulating hormone (FSH), the number of antral follicles, levels of anti-Müllerian hormone (AMH), the total amount of gonadotropin (GN), the time of ovulation induction, the number of eggs obtained, the number of available embryos, and the number of high-quality embryos cycles were compared in different treatment.

Results: The age of female patients did not significantly affect the levels of AMH or FSH during menstruation, nor the number of antral follicles before ovulation induction (P>0.05). However, with an increase in age, an increase in the number of controlled COH cycles was observed. In patients who underwent 2 COH cycles, the number of high-quality embryos in the second cycle increased significantly compared to the first cycle (P<0.05). However, there were no significant differences in the ovulation induction time, the number of eggs, the GN dosage, and the number of available embryos (P>0.05). In patients with 3 treatment cycles, the GN dose used in the third cycle was significantly lower than that used in the first cycle (P<0.05). There were no significant differences in the ovulation induction time, the number of eggs obtained, and the quality of embryos (P>0.05). In patients with 4 treatment cycles, significant differences were observed in the ovulation induction time between the first and the fourth controlled COH cycle (P<0.05). However, no significant differences were detected in GN dosage, ovulation induction time, the number of eggs obtained, the number of available embryos, and the quality of embryos (P>0.05).

Conclusions: Ovarian reserve function was not significantly affected in patients with up to 4 ovarian stimulation cycles.
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http://dx.doi.org/10.21037/apm-21-330DOI Listing
April 2021

Hypermethylation of dihydrofolate reductase promoter increases the risk of hypertension in Chinese.

J Res Med Sci 2020 30;25:117. Epub 2020 Dec 30.

Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, PR China.

Background: DNA methylation was considered to play an important role in hypertension. However, the direct association between dihydrofolate reductase () promoter methylation and hypertension remains unclear. We thus aimed to investigate the relationship between DNA methylation of promoter and hypertension.

Materials And Methods: A total of 371 hypertensive patients (diastolic blood pressure ≥90 mmHg and/or systolic blood pressure ≥140 mmHg or a history of antihypertensive treatment) and 320 age- and sex-matched healthy controls from the Hypertension Management Information System in Nanshan Community Health Service Centers were included in this case-control study. Quantitative methylation-specific polymerase chain reaction was used to measure the level of promoter methylation, which was presented as the percentage of methylated reference (PMR). A multivariate logistic regression model was used to explore the risk of promoter methylation.

Results: Our results indicated that the level of promoter methylation was higher in hypertensive patients (median PMR, 34.32%; interquartile range, 11.34-119.60) than in healthy controls (median PMR, 18.45%; interquartile range, 8.16-35.40) ( < 0.001). Multivariable analysis showed that the risk of promoter hypermethylation was significantly higher in hypertensive patients than in healthy controls (odds ratio = 3.94, 95% confidence interval = 2.56-6.02, < 0.001). Furthermore, hypermethylation was positively associated with sex, high blood homocysteine levels, and alcohol drinking. In particular, the area under the receiver operating characteristic curve was 0.688 (0.585-0.668) for the male hypertensive patients, suggesting the potential diagnostic value of promoter methylation in male hypertension.

Conclusion: Our results demonstrated that promoter hypermethylation is positively associated with the risk of hypertension in Chinese.
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http://dx.doi.org/10.4103/jrms.JRMS_895_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067893PMC
December 2020

GanDTI: A multi-task neural network for drug-target interaction prediction.

Comput Biol Chem 2021 Jun 18;92:107476. Epub 2021 Mar 18.

Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.

Drug discovery processes require drug-target interaction (DTI) prediction by virtual screenings with high accuracy. Compared with traditional methods, the deep learning method requires less time and domain expertise, while achieving higher accuracy. However, there is still room for improvement for higher performance with simplified structures. Meanwhile, this field is calling for multi-task models to solve different tasks. Here we report the GanDTI, an end-to-end deep learning model for both interaction classification and binding affinity prediction tasks. This model employs the compound graph and protein sequence data. It only consists of a graph neural network, an attention module and a multiple-layer perceptron, yet outperforms the state-of-the art methods to predict binding affinity and interaction classification on the DUD-E, human, and bindingDB benchmark datasets. This demonstrates our refined model is highly effective and efficient for DTI prediction and provides a new strategy for performance improvement.
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http://dx.doi.org/10.1016/j.compbiolchem.2021.107476DOI Listing
June 2021

Construction of circRNA-Based ceRNA Network to Reveal the Role of circRNAs in the Progression and Prognosis of Hepatocellular Carcinoma.

Front Genet 2021 26;12:626764. Epub 2021 Feb 26.

Department of General Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

Background: Circular RNAs (circRNAs) are now under hot discussion as novel promising biomarkers for patients with hepatocellular carcinoma (HCC). The purpose of our study is to identify several competing endogenous RNA (ceRNA) networks related to the prognosis and progression of HCC and to further investigate the mechanism of their influence on tumor progression.

Methods: First, we obtained gene expression data related to liver cancer from The Cancer Genome Atlas (TCGA) database (http://www.portal.gdc.cancer.gov/), including microRNA (miRNA) sequence, RNA sequence, and clinical information. A co-expression network was constructed through the Weighted Correlation Network Analysis (WGCNA) software package in R software. The differentially expressed messenger RNAs (DEmRNAs) in the key module were analyzed with the Database for Annotation Visualization and Integrated Discovery (DAVID) (https://david.ncifcrf.gov/summary.jsp) to perform functional enrichment analysis including Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). The data of miRNA expression and clinical information downloaded from TCGA were utilized for survival analysis to detach the prognostic value of the DEmiRNAs of the key module.

Results: The 201 differentially expressed miRNAs (DEmiRNAs) and 3,783 DEmRNAs were preliminarily identified through differential expression analysis. The co-expression networks of DEmiRNAs and DEmRNAs were constructed with WGCNA. Further analysis confirmed four miRNAs in the most significant module (blue module) were associated with the overall survival (OS) of patients with liver cancer, including hsa-miR-92b-3p, hsa-miR-122-3p, hsa-miR-139-5p, and hsa-miR-7850-5p. DAVID was used for functional enrichment analysis of 286 co-expressed mRNAs. The GO analysis results showed that the top enriched GO terms were oxidation-reduction process, extracellular exosome, and iron ion binding. In KEGG pathway analysis, the top three enriched terms included metabolic pathways, fatty acid degradation, and valine, leucine, and isoleucine degradation. In addition, we intersected the miRNA-mRNA interaction prediction results with the differentially expressed and prognostic mRNAs. We found that hsa-miR-92b-3p can be related to CPEB3 and ACADL. By overlapping the data of predicted circRNAs by circBank and differentially expressed circRNAs of GSE94508, we screened has_circ_0077210 as the upstream regulatory molecule of hsa-miR-92b-3p. Hsa_circ_0077210/hsa-miR-92b-3p/cytoplasmic polyadenylation element binding protein-3 (CPEB3) and acyl-Coenzyme A dehydrogenase, long chain (ACADL) were validated in HCC tissue.

Conclusion: Our research provides a mechanistic elucidation of the unknown ceRNA regulatory network in HCC. Hsa_circ_0077210 might serve a momentous therapeutic role to restrain the occurrence and development of HCC.
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http://dx.doi.org/10.3389/fgene.2021.626764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953168PMC
February 2021

S100A7 promotes the development of human endometriosis by activating NF-κB signaling pathway in endometrial stromal cells.

Cell Biol Int 2021 Jun 19;45(6):1327-1335. Epub 2021 Mar 19.

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Endometriosis (EM) is a chronic inflammatory disease affecting women aged between 23 and 42 years with a prevalence of 6%-10%. S100A7, a member of the S100 protein family, has been implicated in promoting inflammation. However, the role of S100A7 in EM and its underlying mechanism remain to be elucidated. S100A7 was silenced or overexpressed in primary endometrial stromal cells (ESCs). Cell proliferation was determined using a Cell Counting Kit-8. Cell cycle/apoptosis was monitored using a flow cytometer. Cell invasion was studied by a Transwell assay. Quantitative RT-PCR and Western blot analyses were used to evaluate gene expression. S100A7 and NF-κB expression is increased in both endometriotic tissue and ESCs from women with EM. The expression of S100A7 is correlated with the expression of NF-κB. S100A7 knockdown inhibits ESCs proliferation, cell cycle progression, cell invasion, and inflammation, but promotes cell apoptosis in an NF-κB dependent manner. In contrast, S100A7 overexpression demonstrated an inverse effect. S100A7 is increased in both endometriotic tissue and ESCs from women with EM. S100A7 overexpression contributes to EM through increasing ESCs proliferation, cell cycle progression, cell invasion, and inflammation, and inhibiting cell apoptosis in the NF-κB dependent manner. These findings highlight the importance of S100A7/NF-κB signaling in EM and provide new insights into therapeutic strategies for EM.
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http://dx.doi.org/10.1002/cbin.11578DOI Listing
June 2021

PAK4 suppresses motor neuron degeneration in hSOD1 -linked amyotrophic lateral sclerosis cell and rat models.

Cell Prolif 2021 Apr 21;54(4):e13003. Epub 2021 Feb 21.

Department of Neurology, The First Clinical College of Harbin Medical University, Harbin, China.

Objectives: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons (MN). CREB pathway-mediated inhibition of apoptosis contributes to neuron protection, and PAK4 activates CREB signalling in diverse cell types. This study aimed to investigate PAK4's effect and mechanism of action in ALS.

Methods: We analysed RNA levels by qRT-PCR, protein levels by immunofluorescence and Western blotting, and apoptosis by flow cytometry and TUNEL staining. Cell transfection was performed for in vitro experiment. Mice were injected intraspinally to evaluate PAK4 function in vivo experiment. Rotarod test was performed to measure motor function.

Results: The expression and activation of PAK4 significantly decreased in the cell and mouse models of ALS as the disease progressed, which was caused by the negative regulation of miR-9-5p. Silencing of PAK4 increased the apoptosis of MN by inhibiting CREB-mediated neuroprotection, whereas overexpression of PAK4 protected MN from hSOD1 -induced degeneration by activating CREB signalling. The neuroprotective effect of PAK4 was markedly inhibited by CREB inhibitor. In ALS models, the PAK4/CREB pathway was inhibited, and cell apoptosis increased. In vivo experiments revealed that PAK4 overexpression in the spinal neurons of hSOD1 mice suppressed MN degeneration, prolonged survival and promoted the CREB pathway.

Conclusions: PAK4 protects MN from degeneration by activating the anti-apoptotic effects of CREB signalling, suggesting it may be a therapeutic target in ALS.
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http://dx.doi.org/10.1111/cpr.13003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016643PMC
April 2021

Diphenyl diselenide protects motor neurons through inhibition of microglia-mediated inflammatory injury in amyotrophic lateral sclerosis.

Pharmacol Res 2021 Mar 27;165:105457. Epub 2021 Jan 27.

Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, PR China. Electronic address:

Microglia-mediated neuroinflammatory response and neuron damage are considered as a self-propelling progressive cycle, being strongly implicated in the progression of neurodegeneration in amyotrophic lateral sclerosis (ALS). Diphenyl diselenide (DPDS), a simple organoselenium compound, has been known to possess multiple pharmacological properties. The purpose of this study was to explore the neuroprotective effects of DPDS against microglia-mediated neuroinflammatory injury in ALS models. We found that DPDS pretreatment inhibited LPS-induced activation of IκB/NF-κB pathway and subsequent release of proinflammatory factors from activated primary hSOD1 microglia. Moreover, DPDS suppressed NLRP3 inflammasome activation by decreasing protein nitration via reduction in NO and ROS levels, whose low levels are related to NF-κB inhibition responsible for iNOS and NOX2 down-regulations, respectively. Notably, DPDS-mediated ROS attenuation was not linked to Nrf2 activation in this cellular model. Furthermore, in the absence of activated microglia, DPDS has no significant effect on the individual hSOD1-NSC34 cells; however, in in vitro neuron-microglia conditional culture and co-culture experiments, DPDS protected motor neurons from neurotoxic damage caused by LPS or BzATP-stimulated microglia activation. Above observations suggest that DPDS-afforded neuroprotection is linked to inhibition of microglia-mediated neuroinflammation in ALS, which was further verified in vivo as shown by improvements of motor deficits, prolonged survival, and reduction of motor neuron loss and reactive microgliosis in hSOD1 transgenic mouse. Altogether, our results show that DPDS elicited neuroprotection in ALS models through inactivation of microglia by inhibiting IκB/NF-κB pathway and NLRP3 inflammasome activation, suggesting that DPDS may be a promising candidate for potential therapy for ALS.
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http://dx.doi.org/10.1016/j.phrs.2021.105457DOI Listing
March 2021

Cardiovascular health behaviours of young adolescents: Results from the global school-based student health survey.

J Paediatr Child Health 2021 04 23;57(4):566-573. Epub 2021 Jan 23.

Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Aim: Levels of American Heart Association-defined cardiovascular (CV) health behaviours have not been fully reported among young adolescents in low- and middle-income countries (LMICs). We describe poor, intermediate and ideal levels of American Heart Association-defined CV health behaviours among young adolescents in LMICs.

Methods: We categorised the levels of CV health behaviours (smoking, body mass index, physical activity and diet) as poor, intermediate, or ideal and calculated the prevalence of each level and the cumulative number of ideal CV health behaviours using the latest data from the global school-based health survey during 2009-2015. The weighted prevalence and 95% confidential intervals were calculated for the whole sample and for sub-groups stratified by gender and age. Pooled overall and regional estimates were calculated using a random-effects model. This study included 153 759 young adolescents from 45 countries.

Results: Overall, 86.3% (95% confidence interval, 82.7-89.9), 80.1% (79.8-80.4), 15.4% (13.7-17.2) and 1.7% (1.1-2.2) of respondents reported ideal levels for smoking, body mass index, physical activity and a healthy diet score, respectively. Overall, 0.3% (0.2-0.4) of respondents had 'four' cumulative ideal CV health behaviours. This 'percentage' was lowest in the Americas (0.2%) and highest in Southeast Asia (0.5%).

Conclusions: Consistently low proportions of young adolescents in LMICs met the ideal levels of physical activity and a healthy diet score or had 'four' ideal CV health behaviours. For this population, physical activity and a healthy dietary pattern should be strongly prioritised.
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http://dx.doi.org/10.1111/jpc.15268DOI Listing
April 2021

Temporal patterns in the first unprovoked seizure.

Epilepsy Behav 2021 02 6;115:107625. Epub 2021 Jan 6.

Department of Medicine, St. Vincent's Hospital, University of Melbourne, Melbourne, Australia; Department of Neuroscience, Monash Medical Centre, Melbourne, Australia; School of Clinical Sciences at Monash Health, Department of Medicine, Monash University, Melbourne, Australia. Electronic address:

Objective: Cyclic phenomena in epilepsy are well recognized. We investigated a multicenter cohort of unprovoked first seizure presentations to determine whether seizures have a preponderance to occur in: a particular time of the day, a particular day of the week, a particular month of the year, day time versus night time, and wakefulness versus sleep.

Methods: We retrospectively studied adults who presented with a first-ever unprovoked seizure to the First Seizure Clinic at two tertiary centers in Australia. Seizure onset time was obtained from the emergency department and ambulance documentations. Electro-clinical and neuroimaging findings were reviewed. We used histograms and Poisson regression modeling to determine whether seizures have a preponderance to occur at a particular time and calculated incidence rate ratios (IRR). We performed further analysis on patients with "first seizure epilepsy" and "first seizure not epilepsy" based on the ILAE criteria for a diagnosis of epilepsy after a single unprovoked seizure, as well as comparing patients that could be categorized as having a generalized-onset seizure versus those with focal-onset seizures.

Results: We analyzed 1724 patients (38% females; age range 14-97 yr, median 39 yr), of whom 18% had epileptiform abnormalities on EEG and potentially epileptogenic lesions were detected on neuroimaging in 28%. Whole cohort analysis shows the incidence rate ratios (IRR) of seizures varied significantly across the 24-hour clock-time of the day (p < 0.001), peaking at hour 12 (IRR 3.18). The first unprovoked seizure was significantly less likely to be reported during the night (IRR 0.61, p < 0.001) and during sleep (IRR 0.29, p < 0.001). Both the "first seizure epilepsy" and "first seizure not epilepsy" subgroups' analysis demonstrated similar patterns. An infraradian pattern was also noted with seizures most likely to occur in May (IRR 1.29, p = 0.02). Both "first seizure epilepsy - generalized" and "first seizure epilepsy - focal" groups had a preponderance for seizures to occur during the day versus night and wakefulness as opposed to sleep, but the association was more robust for generalized seizures.

Conclusions: Our results suggest that temporal patterns are seen in patients with first-ever unprovoked seizures, including those that meet contemporary criteria for epilepsy. These results raise the possibility that first unprovoked seizures have intrinsic rhythmicity similar to epileptic seizures.
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http://dx.doi.org/10.1016/j.yebeh.2020.107625DOI Listing
February 2021

Revisiting an ancient legend: Influence of the lunar cycle on occurrence of first-ever unprovoked seizures.

Intern Med J 2020 Nov 16. Epub 2020 Nov 16.

Department of Medicine, St Vincent's Hospital, University of Melbourne, Melbourne, Australia, 3065.

Background: The mythical effect of the lunar cycle on seizures has been debated over time. Previously healthy individuals presenting with first-ever seizures in whom investigations are negative often invoke questions about potential reasons including a full moon.

Aims: To determine whether there is a temporal relationship between the occurrence of the first-ever unprovoked seizure and the lunar cycle.

Methods: We studied adults who presented with a first-ever unprovoked seizure to two tertiary centres in Australia. Seizure onset time was obtained from the emergency department and ambulance documentations. We used Poisson regression modelling and incidence rate ratios (IRR) to determine whether seizures have a preponderance for a particular lunar phase. We performed further analysis on "first seizure epilepsy" and "first seizure not epilepsy" subgroups based on the International League Against Epilepsy criteria for a diagnosis of epilepsy after a single unprovoked seizure.

Results: We analysed 1710 patients (38% females; median 39 yr), of whom 18% had epileptiform abnormalities on EEG and potentially epileptogenic lesions were detected on neuroimaging in 28%. Based on the EEG and imaging findings, 684 (40%) patients were categorized as "first seizure epilepsy" and 1026 (60%) "first seizure not epilepsy". The whole cohort and subgroup analysis demonstrated no significant difference in the seizure occurrence among the four lunar quarters.

Conclusions: First unprovoked seizures are not influenced by the lunar cycle. Patients pondering the cause of their first-ever unprovoked seizure can be reassured that the full moon was not responsible. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/imj.15135DOI Listing
November 2020

Pain Relief during Oocyte Retrieval by Transcutaneous Electrical Acupoint Stimulation: A Single-Blinded, Randomized, Controlled Multicenter Trial.

Evid Based Complement Alternat Med 2020 22;2020:3285648. Epub 2020 Sep 22.

Center of Reproductive Medicine, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, China.

Acupuncture has pain-relief effects, but no data were available on the use of transcutaneous electric acupoint stimulation (TEAS) in pain relief during oocyte retrieval. This study was designed to examine the effect of TEAS for pain relief in women undergoing transvaginal ultrasound-guided oocyte aspiration. This single-blinded, multicenter, randomized controlled trial was performed in China between May 2013 and May 2015. The subjects were randomized to mock TEAS and TEAS. TEAS or mock TEAS was administered 30 min before oocyte retrieval until the end of the operation. The primary and secondary endpoints were the pain measured using the visual analog scale (VAS) within 1 min and 1 hour after oocyte retrieval, respectively. Serum -endorphin levels were tested in the first 50 patients/group. 390 women were undergoing oocyte retrieval. Pain levels evaluated using VAS within 1 min (18.6 ± 1.3 vs. 24.4 ± 1.7, < 0.01) and 1 h after oocyte aspiration (4.6 ± 0.7 vs. 6.8 ± 0.8, < 0.05) were lower in the TEAS group than in the mock TEAS group. Nausea assessment revealed a significantly lower VAS score in the TEAS group within 1 min (1.2 ± 0.4 vs. 2.9 ± 0.7, < 0.033). Serum -endorphin levels were significantly higher in the TEAS group than in the mock TEAS group (11.4 ± 0.5 vs. 9.1 ± 0.4, < 0.001) after retrieval. Serum -endorphin levels were higher in the TEAS group after the procedure than baseline (11.4 ± 0.5 vs. 9.1 ± 0.3, < 0.001). Oocyte retrieval causes pain and discomfort, but TEAS is effective and safe for suppressing the pain and alleviating nausea associated with the operation.
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http://dx.doi.org/10.1155/2020/3285648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530499PMC
September 2020

Delayed removal of a percutaneous left ventricular assist device for patients undergoing catheter ablation of ventricular tachycardia is associated with increased 90-day mortality.

J Interv Card Electrophysiol 2020 Sep 19. Epub 2020 Sep 19.

Division of Cardiac Electrophysiology, Texas Heart Institute, MC 2-225, PO BOX 20345, Houston, TX, 77225-0345, USA.

Purpose: Assess if timing of removal of a percutaneous left ventricular assist device (pLVAD) after ventricular tachycardia (VT) ablation alters patient outcomes.

Methods: Sixty-nine patients underwent pLVAD support. Patients were divided into early (< 24 h, n = 43) and delayed (≥ 24 h, n = 26) removal groups after ablation. Factors for delayed pLVAD removal and predictors of 90-day mortality were analyzed.

Results: The delayed removal group had lower LVEF (27.1 ± 9.3% vs. 20.6 ± 5.4%, p = 0.002), greater percentage LVEF < 25% (58.1% vs. 84.6%, p = 0.02), and more VT storm (41.9% vs. 96.2%, p < 0.001). Ventricular fibrillation (VF) was induced in 9/69 (13%), with incidence higher in delayed removal group (27% vs. 5%, p = 0.002). VT storm (OR = 34.72, 95% CI, 4.30-280.33; p = 0.001), LVEF < 25% (OR = 3.95, 95% CI, 1.16-13.48; p = 0.03), and VF induced during ablation (OR = 9.25, 95% CI, 1.71-50.0; p = 0.01) were associated with delayed pLVAD removal in univariate analysis. Delayed pLVAD removal had a significantly higher 90-day mortality rate (2.3% vs 30.2%; p < 0.001). Univariate Cox proportional hazard regression analysis revealed delayed pLVAD removal was a significant predictor of 90-day mortality.

Conclusions: Prolonged pLVAD insertion (≥ 24 h) after VT ablation was associated with VT storm, LVEF < 25%, and VF induced during ablation. Delayed pLVAD removal was a significant predictor of 90-day mortality in patients undergoing VT ablation.
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http://dx.doi.org/10.1007/s10840-020-00875-yDOI Listing
September 2020

Prevalence of oligomenorrhea among women of childbearing age in China: A large community-based study.

Womens Health (Lond) 2020 Jan-Dec;16:1745506520928617

Department of Obstetrics and Gynecology, Reproductive Medical Center, Peking University Third Hospital, Beijing, China.

Objective: To investigate the prevalence and the related characteristics of oligomenorrhea among women within childbearing age in China.

Study Design: A large-scale community-based investigation was conducted from 2013 to 2015. A total of 12,964 women aged 18-49 years from 9 provinces/municipalities in China were recruited for healthcare screening in local community health centers. Outcome measures include clinical history, ultrasonographic exam, and hormonal and metabolic parameters.

Results: Among women within childbearing age in China, the prevalence of oligomenorrhea was 12.2% (1,579/12,964). Both sociodemographic factors and medical history were significantly associated with oligomenorrhea ( < 0.05). In such women, the prevalence of obesity, acne, seborrhea, acanthosis, larger ovarian size, and polycystic ovarian morphology was higher when compared with normal women; the prevalence of anti-Mullerian hormone, total testosterone, and androstenedione ( < 0.05) was higher as well. The infertility rates of all women were higher in the oligomenorrhea group (17.2%, 272⁄1,579) than in the non-oligomenorrhea group (9.0%, 1,024⁄11,385), and among women without contraception, for the oligomenorrhea group, the infertility rate was 32.5% (128⁄394), and for the non-oligomenorrhea group, 17.9% (400⁄2,240). In the oligomenorrhea group, 57.4% (156/272) of the women underwent treatments for infertility, which was higher than the non-oligomenorrhea group 36.1% (370/1,024).

Conclusions: Obesity, acne, seborrhea, acanthosis, larger ovarian size, and polycystic ovarian morphology were significantly associated with oligomenorrhea. The increase of anti-Mullerian hormone, total testosterone, and androstenedione level was also demonstrated in the oligomenorrhea group. Higher prevalence of infertility and medical treatment rate was observed in women with oligomenorrhea.
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http://dx.doi.org/10.1177/1745506520928617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479865PMC
September 2020

Catalytic ozonation of iohexol with α-FeMnOOH in water: Efficiency, degradation mechanism and toxicity evaluation.

J Hazard Mater 2021 01 26;402:123574. Epub 2020 Jul 26.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, PR China. Electronic address:

Iohexol, a widely used iodinated X-ray contrast media, is difficult to completely degrade with the traditional water treatment process. Catalytic ozonation with synthesized α-FeMnOOH as the catalyst can significantly promote the degradation of iohexol relative to that with ozonation alone. Hydroxyl radicals play a predominant role during the degradation of iohexol. The effect of various factors, including catalyst dose, ozone dose, iohexol concentration and water matrix factors, on the catalytic performance were investigated. The presence of α-FeMnOOH in the catalytic system can significantly promote the removal of iohexol and mineralization of the dissolved organic carbon in real water samples. The intermediate products were determined by high-resolution liquid chromatography, and the reaction site was predicted by frontier electron density (FED) calculations. The degradation mechanism of iohexol followed the processes of H-abstraction, amide hydrolysis, amide oxidation, and ·OH substitution. Higher exposure concentrations of iohexol had a negative effect on the survival and hatching rates in the development of zebrafish embryos. The autonomic movement process and heartbeat rate of the zebrafish larvae showed significant differences as the exposure concentration of iohexol increased. The catalytic ozonation process with α-FeMnOOH can decrease the toxicity of iohexol containing water.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123574DOI Listing
January 2021

Polystyrene nanoplastics cause growth inhibition, morphological damage and physiological disturbance in the marine microalga Platymonas helgolandica.

Mar Pollut Bull 2020 Sep 2;158:111403. Epub 2020 Jul 2.

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address:

Effects of nanoplastics at low level on the marine primary producer are largely unclear. To assess the potential risk of nanoplastic pollution, this study exposed marine green microalgae Platymonas helgolandica to 20, 200, and 2000 μg/L 70-nm polystyrene nanoplastics for 6 days. Nanoplastics significantly inhibited the growth of P. helgolandica during the first 4 days of exposure, and elevated heterocyst frequency was observed in 200 and 2000 μg/L exposure groups in the early exposure stage. Exposure to 200 and 2000 μg/L nanoplastics for 4 days increased the membrane permeability and mitochondrial membrane potential, and decreased light energy used in photochemical processes of microalgae. Moreover, clear morphological changes, including surface folds, fragmentation, aggregation cluster, and rupture, in the microalgae exposed to nanoplastics were observed under scanning electron microscope and transmission electron microscope. These results demonstrate that nanoplastics could reduce the microalgal vitality by the damage on cell morphology and organelle function.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111403DOI Listing
September 2020

Skipping breakfast and physical fitness among school-aged adolescents.

Clinics (Sao Paulo) 2020 22;75:e1599. Epub 2020 Jul 22.

Department of Epidemiology, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, China.

Objectives: This study explored the relationship between skipping breakfast and physical fitness in a group of school-aged adolescents in China.

Methods: This cross-sectional study from the Chinese National Surveillance on Students' Constitution and Health (CNSSCH) survey in Ningbo, China, used a standardized questionnaire to assess the frequency of breakfast consumption. Physical fitness was measured through standing long jump, 50-m sprint, 1,000 (or 800)-m run, and vital capacity tests. Multiple linear regression analysis was used to investigate the relationship between the frequency of breakfast consumption and physical fitness.

Results: Our study included a total of 1,849 school-aged adolescents (aged 15.53±1.80 years). Among boys, non-breakfast-skippers had good scores for 50-m sprints, 1,000-m run, and vital capacity tests when compared with breakfast skippers (all p<0.05). Among girls, non-breakfast-skippers had a good scores for the standing long jump test compared with breakfast skippers (p=0.003). The multiple linear regression model showed that not skipping breakfast was positively associated with vital capacity (β=-173.78, p=0.004) and inversely associated with 50-m sprint (β=-0.12, p=0.018) and 1,000-m run times (β=-8.08, p=0.001) in boys.

Conclusion: The results of this cross-sectional study revealed that skipping breakfast might be associated with lower physical fitness in Chinese adolescents aged 13-18 years, especially boys. Breakfast consumption should be promoted among Chinese school-aged boys.
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http://dx.doi.org/10.6061/clinics/2020/e1599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362721PMC
November 2020

Salpingectomy may decrease antral follicle count but not live birth rate for IVF-ET patients aged 35-39 years: a retrospective study.

J Ovarian Res 2020 Jul 20;13(1):80. Epub 2020 Jul 20.

Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, 251 Yao jia yuan Road, Chaoyang District, Beijing, 100026, China.

Purpose: Problems with fallopian tubes are one of the main reasons for women to undergo in vitro fertilization-embryo transfer (IVF-ET). A large proportion of women with ectopic pregnancy, fallopian tube obstruction and hydrosalpinx have had one or both fallopian tubes removed by salpingectomy. With increasing age, ovarian reserve deteriorates, the numbers of retrieved oocytes, available embryos and high-quality embryos are reduced, and the live birth rate for women treated with IVF treatment is affected. Thus, it is important to understand how salpingectomy affects live birth rates for IVF patients of different ages. This study analyzed how patients' age and salpingectomy influenced ovarian reserve, ovarian response and pregnancy outcomes for infertile women undergoing IVF-ET.

Methods: A total of 1922 patients that underwent IVF-ET treatment from January 1, 2012, to December 31, 2018, were included in this retrospective study. The patients were divided into two groups according to whether or not they had a previous history of salpingectomy. The salpingectomy (group A, 534 patients) and control groups (group B, 1388 patients) were then further divided into two subgroups according to patient age (age<35 years, and age 35-39 years). Ovarian reserve, ovarian response, and IVF outcomes were investigated for each subgroup. Logistic regression model was used to estimate the relationship between clinical pregnancy and live births and patients' baseline characteristics.

Results: In the salpingectomy group, antral follicle counts (AFC) were significantly lower for the subgroup aged 35 to 39 years compared with the control group. But this difference did not appear in women younger than 35 years. In addition, there were no significant differences in levels of basal follicle stimulation hormone (FSH), basal luteinizing hormone (LH), basal estradiol (E), total gonadotropins (Gn) dose, duration of Gn, numbers of retrieved oocytes, fertilization rates, numbers of available embryos, live birth rates, clinical pregnancy rates, miscarriage rates, ectopic pregnancy rates, or multiple pregnancy rates between the salpingectomy group and the control group (P > 0.05). Age is a risk factor for the clinical pregnancy and live birth.

Conclusion: Salpingectomy may decrease antral follicle count but not live birth rate for IVF-ET patients aged 35-39 years. The increased female age was negative related with clinical pregnancy and live birth.
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http://dx.doi.org/10.1186/s13048-020-00678-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370502PMC
July 2020

Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function.

J Cell Mol Med 2020 09 15;24(17):9658-9666. Epub 2020 Jul 15.

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Endometriosis is a common, chronic gynaecologic disease affecting up to 10% of women in their reproductive age and leading to pain and infertility. Oestrogen (E )-induced epithelial-mesenchymal transition (EMT) process has been considered as a key factor of endometriosis development. Recently, the dysregulated circular RNAs (circRNAs) have been discovered in endometriosis tissues. However, the molecular mechanism of circRNAs on the E -induced EMT process in endometriosis is still unknown. Here, we demonstrated that circ_0004712 up-regulated by E treatment in endometrial epithelial cells. Knock-down the expression of circ_0004712 significantly suppressed E -induced cell migration activity. Meanwhile, we identified miR-148a-3p as a potential target miRNA of circ_0004712. Inhibited the expression of miR-148a-3p could recovered the effect of circ_0004712 knock-down in E -treated endometrial epithelial. Furthermore, Western blot assay showed that E treatment could increase the expression and activity of β-catenin, snail and N-cadherin and reduce the expression of E-cadherin. The expression and activity of β-catenin pathway were recovered by circ_0004712 knock-down or miR-148a-3p overexpression. Altogether, the results demonstrate that circ_0004712/miR-148a-3p plays an important role in E -induced EMT process in the development of endometriosis, and the molecular mechanism may be associated with the β-catenin pathway. This work highlighted the importance of circRNAs in the development of endometriosis and provide a new biomarker for diagnosis and therapies.
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http://dx.doi.org/10.1111/jcmm.15495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520264PMC
September 2020

The role of serum periostin in the diagnosis of asthma: A meta-analysis.

Allergy Asthma Proc 2020 07;41(4):240-247

From the Department of Pediatrics, Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei, People's Republic of China; and.

Serum periostin has been proposed as a noninvasive biomarker for asthma diagnosis and management. However, its accuracy for the diagnosis of asthma in different populations is not completely clear. This meta-analysis aimed to evaluate the diagnostic accuracy of periostin level in the clinical determination of asthma. Several medical literature data bases were searched for relevant studies through December 1, 2019. The numbers of patients with true-positive, false-positive, false-negative, and true-negative results for the periostin level were extracted from each individual study. We assessed the risk of bias by using Quality Assessment of Diagnostic Accuracy Studies 2. We used the meta-analysis to produce summary estimates of accuracy. In total, nine studies with 1757 subjects met the inclusion criteria. The pooled estimates of sensitivity, specificity, and diagnostic odds ratios for the detection of asthma were 0.58 (95% confidence interval [CI], 0.38-0.76), 0.86 (95% CI, 0.74-0.93), and 8.28 (95% CI, 3.67-18.68), respectively. The area under the summary receiver operating characteristic curve was 0.82 (95% CI, 0.79-0.85). And significant publication bias was found in this meta-analysis (p = 0.39). Serum periostin may be used for the diagnosis of asthma, with moderate diagnostic accuracy.
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http://dx.doi.org/10.2500/aap.2020.41.200038DOI Listing
July 2020

Antinuclear antibodies in follicular fluid may reduce efficacy of in vitro fertilization and embryo transfer by invading endometrium and granular cells.

Am J Reprod Immunol 2020 10 6;84(4):e13289. Epub 2020 Jul 6.

Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Problem: The mechanism(s) by which antinuclear antibodies (ANA) induce implantation failure are not clear, and little information regarding the function of autoantibodies in reproductive tissues is available.

Methods Of Study: A total of 380 patients who underwent in vitro fertilization and embryo transfer (IVF-ET) were divided into control, serum positive, and follicular fluid (FF) positive groups based on the results of indirect immunofluorescence assay for ANA in the serum and FF. Immunofluorescence assay was performed to evaluate the existence of ANA in granular cells and endometrial tissues. Presence in FF of soluble apoptotic markers, including Bcl-2, Caspase-3, cleaved PARP, Cytochrome C, GAPDH, and p53, was assessed using magnetic bead based assays.

Results: The patients in the FF positive group had the lowest numbers of retrieved oocytes, fertilizations, and high-quality embryos. The fertilization rate, and the proportion of two pronuclear (2PN) embryos in patients in the FF positive group were significantly lower than those in the other two groups. The FF positive group also had the lowest clinical pregnancy rate, and the highest early miscarriage rate. Granulosa cells and endometrial tissues in patients in the FF positive group were ANA positive. High levels of BCL-2, Caspase-3, Cytochrome C, GAPDH, and p53 were found in the FF of patients in the FF positive group.

Conclusions: Antinuclear antibodies in FF and endometrial tissues may cause imbalanced apoptosis, resulting in poor IVF-ET treatment outcomes. Local autoimmunity and cell apoptosis in reproductive tissues could be considered new therapeutic targets for improving IVF-ET treatment efficacy.
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http://dx.doi.org/10.1111/aji.13289DOI Listing
October 2020

LncRNA SNHG4 promotes the increased growth of endometrial tissue outside the uterine cavity via regulating c-Met mediated by miR-148a-3p.

Mol Cell Endocrinol 2020 08 7;514:110887. Epub 2020 Jun 7.

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, 100026, PR China. Electronic address:

Long noncoding RNAs (lncRNAs) modulate endometriosis. The current study investigated the mechanisms and effects of SNHG4 on endometriosis. The qRT-PCR was conducted to examine the miR-148a-3p and SNHG4 expressions in endometriosis tissues. The 5-ethynyl-2'-deoxyuridine incorporation assay and 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide assay were used to measure the rate of cell proliferation. The association between miR-148a-3p, SNHG4 and c-Met was confirmed via bioinformatical approach and luciferase reporter gene assay. Also, the function of SNHG4 on the growth of endometriotic lesions was investigated in vivo. The SNHG4 expression was considerably upregulated in endometriosis tissues, whereas the level of miR-148a-3p expression was reduced. In addition, SNHG4 can be considered as ceRNAs that bind miR-148a-3p and rise the proliferation activity of HESCs by downregulating miR-148a-3p. Furthermore, silencing SNHG4 could downregulate the c-Met level by enhancing miR-148a-3p expression, and finally inhibiting endometriosis development in vivo. LncRNA SNHG4 promotes the increased growth of endometrial tissue outside the uterine cavity via regulating c-Met mediated by miR-148a-3p, which may be used as diagnostic biomarker as well as molecular target in the treatment of endometriosis.
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http://dx.doi.org/10.1016/j.mce.2020.110887DOI Listing
August 2020

Significant association between DHFR promoter methylation and ischemic stroke in a Chinese hypertensive population.

J Clin Lab Anal 2020 Aug 22;34(8):e23322. Epub 2020 Apr 22.

Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, China.

Objective: DHFR encodes dihydrofolate reductase, a major enzyme in the metabolism of folate, and is a candidate gene for ischemic stroke (IS). Therefore, we aimed to investigate the association between DHFR promoter methylation and IS in a Chinese population with primary hypertension.

Methods: Quantitative methylation-specific PCR was used to measure the level of DHFR promoter methylation. A multivariate logistic regression model was used to investigate the association between DHFR promoter methylation and IS. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of DHFR promoter methylation for IS.

Results: The level of methylation of the DHFR promoter in the IS group was significantly lower than that in the hypertensive group (median [interquartile range]: 9.11 [2.81-16.20] vs 24.94 [7.16-56.45], P < .001). DHFR promoter methylation and homocysteine (Hcy) levels were both related to IS, with an ORs (95% CI) of 0.976 (0.967-0.984) and 1.057 (1.027-1.108), respectively. The areas under the curve for the diagnosis of DHFR promoter hypomethylation in IS were 0.603 (95% CI, 0.527-0.678) in men and 0.754 (95% CI, 0.693-0.815) in women. A dual-luciferase reporter assay revealed that the target sequence in the DHFR promoter upregulated gene expression.

Conclusion: There is a significant association between methylation of the DHFR promoter and IS in this Chinese hypertensive population. Hypomethylation of the DHFR promoter may serve as a novel marker for the diagnosis of IS in women.
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http://dx.doi.org/10.1002/jcla.23322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439332PMC
August 2020

Loss of hsa_circ_0118530 inhibits human granulosa-like tumor cell line KGN cell injury by sponging miR-136.

Gene 2020 Jun 24;744:144591. Epub 2020 Mar 24.

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, PR China. Electronic address:

Polycystic ovary syndrome (PCOS) is a kind of endocrine disease among women across the global. Recently, many researches have reported circular RNAs can act as significant molecular biomarkers for diseases, especially in tumors. Several Circular RNAs are reported to be aberrantly expressed in PCOS patients. Here, we investigated the biological effects of hsa_circ_0118530 on human granulosa cells, KGN. We observed that hsa_circ_0118530 was greatly elevated in PCOS patients and granulosa cells (including KGN and COV434 cells) compared to normal IOSE80 cells. hsa_circ_0118530 siRNA was transfected into KGN cells. We found that KGN cell viability was repressed, cell apoptosis was induced while cell migration was greatly inhibited. TGF-β1 was utilized to induce EMT process. As shown, loss of hsa_circ_0118530 significantly enhanced E-cadherin mRNA and protein levels while depressed N-cadherin expression. Furthermore, we indicated that decrease of hsa_circ_0118530 was able to inhibit ROS accumulation, MDA levels while induced SOD activity. Next, it was demonstrated that releases of inflammatory cytokine were suppressed by hsa_circ_0118530 down-regulation. Additionally, miR-136 was predicted and confirmed as the target of hsa_circ_0118530. For another, the functions of hsa_circ_0118530 on KGN cell progression, oxidative stress and inflammation releases were obviously reversed by miR-136 suppression. In conclusion, knockdown of hsa_circ_0118530 repressed PCOS progression via sponging miR-136.
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http://dx.doi.org/10.1016/j.gene.2020.144591DOI Listing
June 2020

Diagnostic accuracy of periostin in asthma: question before celebration.

J Asthma 2021 Jul 18;58(7):892. Epub 2020 Mar 18.

Department of Pediatrics, Bethune International Peace Hospital of Chinese PLA, Shijiazhuang, Hebei, People's Republic of China.

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http://dx.doi.org/10.1080/02770903.2020.1740248DOI Listing
July 2021

A comparative analysis on thermal runaway behavior of Li (NiCoMn) O battery with different nickel contents at cell and module level.

J Hazard Mater 2020 Jul 21;393:122361. Epub 2020 Feb 21.

State Key Laboratory of Automotive Safety and Energy, Tsinghua University, Beijing 100084, China. Electronic address:

The problem of thermal runaway (TR) propagation challenges the safety design of battery packs, because it aggravates the thermal hazards to accidents. There are many unsolved scientific questions in understanding the mechanisms of TR and its propagation behavior for large format lithium-ion batteries (LIBs). LiNiCoMnO(NCM) is considered as one of the most promising cathode materials for lithium-ion batteries LIBs, given its higher energy design and lower cost. However, higher Nickel (Ni) content of cathode material worsens the thermal stability of LIBs. This paper provides a comparative analysis on the TR propagation behavior of NCM battery with different Ni ratios. Results have shown that when the characteristic temperatures of TR {T, T, T}and the specific electrochemical energy of the cell are similar, TR propagation behavior will be similar, no matter what kinds of chemistry the cell has. Observation suggests that the average propagation time within a large format cell is 7-10 s in module tests. Besides, the internal temperature of the cell has an order of NCM622 ≥ NCM523 ≥ NCM111,whereas the mass is ordered by NCM622 > NCM523 > NCM111.This work firstly reports the TR feature in large format LIBs with different Ni ratios, both at cell and module level, providing the guidelines for engineering practice and further theoretical researches.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122361DOI Listing
July 2020

Live birth rate comparison of three controlled ovarian stimulation protocols for in vitro fertilization-embryo transfer in patients with diminished ovarian reserve after endometrioma cystectomy: a retrospective study.

J Ovarian Res 2020 Feb 29;13(1):23. Epub 2020 Feb 29.

Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, 251 Yao jia yuan Road, Chaoyang District, Beijing, 100026, China.

Background: Women with endometriosis and previous cystectomy may respond less well to gonadotropin stimulation, which results in fewer oocytes retrieved and poor pregnancy outcomes. Choosing an appropriate protocol for such populations is essential. This study involved an analysis of the effect of different controlled ovarian stimulation (COS) protocols on the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) in women with diminished ovarian reserve (DOR) who underwent ovarian endometrioma cystectomy.

Methods: A total of 342 patients that underwent IVF-ET treatment at the Beijing Obstetrics and Gynecology Hospital from January 1, 2013 to April 30, 2018 were included in this retrospective study. The patients were distributed into three groups according to the COS protocols, namely prolonged GnRH-agonist (Group A, n = 113), GnRH-antagonist (Group B, n = 121), and long GnRH-agonist (Group C, n = 108). The clinical and laboratory parameters of the three protocols were analyzed and a logistic regression of clinical pregnancy and live births was conducted.

Results: There were no significant differences in the age, infertility duration, basic follicle stimulation hormone (FSH), luteinizing hormone (LH), or estradiol (E) levels as well as other baseline characteristics among groups (P > 0.05). The total gonadotrophin (Gn) dosage and duration tended to be less in the GnRH-antagonist group than in the others (P < 0.05). No significant differences were found in the implantation rate and clinical pregnancy rate among the groups, but the prolonged GnRH-agonist group showed the highest rates. In addition, no significant differences were present in the number of retrieved oocytes, oocyte fertilization rate, embryo utilization rate, live birth rate, abortion rate, ectopic pregnancy rate, or multiple pregnancy rate in the three groups (P > 0.05). Age had a significant effect on both clinical pregnancy and live birth.

Conclusion: For those DOR patients who had undergone ovarian endometriosis cystectomy, the prolonged GnRH-agonist protocol may achieve better clinical IVF-ET outcomes, but there were no significant differences from the other groups. The GnRH-antagonist protocol may reduce the cost and time of drug treatment. Age should be considered for its influence on pregnancy outcome. However, a larger sample size may be needed for further study.
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http://dx.doi.org/10.1186/s13048-020-00622-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049193PMC
February 2020

Nanocalorimeters for biomolecular analysis and cell metabolism monitoring.

Biomicrofluidics 2020 Jan 31;14(1):011503. Epub 2020 Jan 31.

Department of Control Engineering, Northeastern University, Qinhuangdao, Hebei 066001, People's Republic of China.

Nanocalorimeters, or microfabricated calorimeters, provide a promising way to characterize the thermal process of biological processes, such as biomolecule interactions and cellular metabolic activities. They enabled miniaturized heat measurement onto a chip device with potential benefits including low sample consumption, low cost, portability, and high throughput. Over the past few decades, researchers have tried to improve nanocalorimeters' performance, in terms of sensitivity, accuracy, and detection resolution, by exploring different sensing methods, thermal insulation techniques, and liquid handling methods. The enhanced devices resulted in new applications in recent years, and here we have summarized the performance parameters and applications based on categories. Finally, we have listed the current technical difficulties in nanocalorimeter research and hope for future solutions to overcome them.
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http://dx.doi.org/10.1063/1.5134870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994269PMC
January 2020

Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage.

J Mol Cell Biol 2020 06;12(5):372-385

Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Chemotherapeutic agents are extensively used to treat malignancies. However, chemotherapy-induced ovarian damage and reduced fertility are severe side effects. Recently, stem cell transplantation has been reported to be an effective strategy for premature ovarian insufficiency (POI) treatment, but safety can still be an issue in stem cell-based therapy. Here, we show the protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium (hUCMSC-CM) on a cisplatin (Cs)-induced ovarian injury model. hUCMSC-CM can relieve Cs-induced depletion of follicles and preserve fertility. In addition, hUCMSC-CM can decrease apoptosis of oocytes and granulosa cells induced by Cs. RNA sequencing analysis reveals the differentially expressed genes of ovaries after Cs and hUCMSC-CM treatments, including genes involved in cell apoptosis. Furthermore, we show that the granulocyte colony-stimulating factor (G-CSF)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway plays an important role in protecting granulosa cells from Cs-induced apoptosis. Together, we confirm the protective effects of hUCMSC-CM on ovarian reserve and fertility in mice treated with Cs, highlighting the remarkable therapeutic effects of hUCMSC-CM.
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http://dx.doi.org/10.1093/jmcb/mjz105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288746PMC
June 2020