Publications by authors named "Shuyang Li"

23 Publications

  • Page 1 of 1

Effects of bovine cancellous bone powder/poly amino acid composites on cellular behaviors and osteogenic performances.

Biomed Mater 2021 Jul 6;16(5). Epub 2021 Jul 6.

School of Automation and Information Engineering, Sichuan University of Science and Engineering, Zigong, People's Republic of China.

Xenogeneic bone has good biological activity, but eliminating immunogenicity, while retaining osteogenic abilities, is a challenge. By combining xenogeneic bone with poly amino acid (PAA) that has an amide bond structure, a new type of composite conforming to bionics and low immunogenicity may be obtained. In this study, according to the principles of component bionics, three composites of delipidized cancellous bone powder (DCBP) and PAA were designed and obtained by anpolycondensation method, an extrusion molding (EM) method, and a solution-blend method. The three composites were all macroscopically uniform, non-cytotoxic, and demonstrated low immunogenicity by effective removal of residual antigens during preparation. Compared with PAA, mouse bone marrow mesenchymal stem cells (BMSCs) on the surfaces of three composites showed different cellular morphologies. The effects of different preparation methods and cellular morphology on cellular differentiation were confirmed by alkaline phosphatase activity, calcium nodule formation and the expression levels of osteogenic differentiation-related genes (bone morphogenetic protein 2, runt-related transcription factor 2, osteopontin and osteocalcin). Among these composites, DCBP/PAA EM showed best cell proliferation and osteogenic differentiation, and possessed greater bone formation than PAA in a rabbit femoral condyle study. This study may provide a new method for preparing bioactive bone repair materials with low immunogenicity and superior ability to stimulate differentiation of BMSCsand osteogenesis. DCBP/PAA EM might be a promising bone repair material for bone defect treatment.
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http://dx.doi.org/10.1088/1748-605X/ac0d94DOI Listing
July 2021

Biological evaluation of the modified nano-amorphous phosphate calcium doped with citrate/poly-amino acid composite as a potential candidate for bone repair and reconstruction.

J Mater Sci Mater Med 2021 Jan 25;32(1):16. Epub 2021 Jan 25.

College of Physics, Sichuan University, Chengdu, 610064, China.

Large numbers of research works related to fabricating organic-inorganic composite materials have been carried out to mimic the natural structure of bone. In this study, a new modified n-ACP doped with citrate (n-ACP-cit)/poly (amino acids) (PAA) composite (n-ACP-cit/PAA) was synthesized by employing high bioactive n-ACP-cit and the biodegradable and biocompatible PAA copolymer. Its basic structure was characterized by X-ray diffraction spectroscopy, Fourier transformed infrared spectroscopy, and X-ray photoelectron spectroscopy. Moreover, the degradability, bioactivity, biocompatibility, and osteoconductivity of n-ACP-cit/PAA composite were evaluated in vitro and in vivo, using simulated body fluid (SBF) solution soaking test, mouse bone marrow mesenchymal stem cells proliferation and differentiation, morphological observation test, expression of genes associated with osteogenesis, and bone defect model repair test, respectively. The modified n-ACP-cit/PAA composite exhibited a much higher weight loss rate (36.01 wt.%) than that of PAA (23.99 wt.%) after immersing in SBF solution for 16 weeks and the pH values of local environment restored to neutral condition. Moreover, cells co-culturing with composites exhibited higher alkaline phosphatase activity, more calcium nodule-formation, and higher expression levels of osteogenic differentiation-related genes (Bmp-2, Colla I, OCN, OPN, and Runx-2) than that of PAA. Furthermore, the bone defect model repair test revealed that the composite could be intimately incorporated with the surrounding bone without causing any deleterious reaction and capable of guiding new bone formation. Together, these results indicated that the new modified bone repair n-ACP-cit/PAA composite material with specific characteristics may be designed for meeting diverse requirements from biomedical applications.
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http://dx.doi.org/10.1007/s10856-020-06482-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829244PMC
January 2021

Preparation of a novel bovine cancellous bone/poly-amino acid composite with low immunogenicity, proper strength, and cytocompatibility in vitro.

J Biomed Mater Res A 2021 Aug 7;109(8):1490-1501. Epub 2020 Dec 7.

College of Physics, Sichuan University, Chengdu, Sichuan, China.

In this work, the delipidized and deproteinized bovine cancellous bone powder/poly-amino acid (DDBP/PAA) composite was fabricated by extrusion-injection molding method for the first time. After about 70% clearance rate by the delipidization and deproteinization procedures, the residual antigens of galactosyl α-(1, 3)-galactosyl β-1,4-N-aeetylglueosaminyl (α-Gal) and major histocompatibility complex (MHC) II were basically eliminated by the extrusion-injection molding process, which may cause high titer of antibody and lead to hyperacute rejection or chronic immune toxicity. Meanwhile, the natural BMP II and apatite in bovine bone were kept in DDBP/PAA composite. After 26 weeks of immersion in simulated body fluid, the DDBP/PAA composite remained the intact appearance, 96.4% of weight, and 69.2% of compressive strength, and these showed sufficient degradation stability. The composite also exhibited excellent attachment and proliferation abilities of mouse bone marrow mesenchymal stem cells (mMSCs). The results herein suggested that the DDBP/PAA composite was expected to be a load-bearing transplant with some natural ingredients for hard tissue repair.
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http://dx.doi.org/10.1002/jbm.a.37139DOI Listing
August 2021

Dissimilatory Nitrate Reduction to Ammonium (DNRA) and Denitrification Pathways Are Leveraged by Cyclic AMP Receptor Protein (CRP) Paralogues Based on Electron Donor/Acceptor Limitation in Shewanella loihica PV-4.

Appl Environ Microbiol 2021 01 4;87(2). Epub 2021 Jan 4.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China

Under anoxic conditions, many bacteria, including strain PV-4, could use nitrate as an electron acceptor for dissimilatory nitrate reduction to ammonium (DNRA) and/or denitrification. Previous and current studies have shown that DNRA is favored under higher ambient carbon-to-nitrogen (C/N) ratios, whereas denitrification is upregulated under lower C/N ratios, which is consistent with our bioenergetics calculations. Interestingly, computational analyses indicate that the common cyclic AMP receptor protein (designated CRP1) and its paralogue CRP2 might both be involved in the regulation of two competing dissimilatory nitrate reduction pathways, DNRA and denitrification, in PV-4 and several other denitrifying species. To explore the regulatory mechanism underlying the dissimilatory nitrate reduction (DNR) pathways, nitrate reduction of a series of in-frame deletion mutants was analyzed under different C/N ratios. Deletion of could accelerate the reduction of nitrite to NO under both low and high C/N ratios. CRP1 is not required for denitrification and actually suppresses production of NO and NO gases. Deletion of either of the NO-forming nitrite reductase genes or blocked production of NO gas. Furthermore, real-time PCR and electrophoretic mobility shift assays (EMSAs) demonstrated that the transcription levels of DNRA-relevant genes such as -β (), , and were upregulated by CRP1, while transcription was dependent on CRP2. There are tradeoffs between the different physiological roles of nitrate/lactate, as nitrogen nutrient/carbon source and electron acceptor/donor and CRPs may leverage dissimilatory nitrate reduction pathways for maximizing energy yield and bacterial survival under ambient environmental conditions. Some microbes utilize different dissimilatory nitrate reduction (DNR) pathways, including DNR to ammonia (DNRA) and denitrification pathways, for anaerobic respiration in response to ambient carbon/nitrogen ratio changes. Large-scale industrial nitrogen fixation and fertilizer application raise the concern of emission of NO, a stable gas with potent global warming potential, as consequence of microbial respiration, thereby aggravating global warming and climate change. However, little is known about the molecular mechanism underlying the choice of two competing DNR pathways. We demonstrate that the global regulator CRP1, which is widely encoded in bacteria, is required for DNRA in PV-4 strain, while the CRP2 paralogue is required for transcription of the nitrite reductase gene for denitrification. Sufficient carbon source lead to the predominance of DNRA, while carbon source/electron donor deficiency may result in an incomplete denitrification process, raising the concern of high levels of NO emission from nitrate-rich and carbon source-poor waters and soils.
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http://dx.doi.org/10.1128/AEM.01964-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783327PMC
January 2021

Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire (JOACMEQ) in mainland China: an investigation of reliability, validity, and responsiveness.

Health Qual Life Outcomes 2020 Oct 22;18(1):349. Epub 2020 Oct 22.

Department of Orthopedics, Peking University Third Hospital, Beijing, China.

Background: The aim of this study is to investigate the reliability, validity, and responsiveness of JOACMEQ for CSM patients in mainland China.

Methods: A retrospective review was performed on 91 patients with CSM in our hospital from March 2015 to June 2015. Patients completed the JOACMEQ, the mJOA and the SF-36 questionnaires during the process. Cronbach's α was used to evaluate the internal consistency reliability, and test-retest reliability was checked. An exploratory factor analysis was used to determine the correlations among the JOACMEQ questions and the construct validity. The concurrent validity was assessed by Spearman correlation coefficient. The internal responsiveness was determined by effect sizes and standardized response means. External responsiveness was determined by the area under the receiver operating characteristic curve on the basis of the Youden Index.

Results: The mean age of patients was 57.61 years old. The mean follow-up was 24 months. JOACMEQ showed a good internal consistency (Cronbach's α, 0.897). Test-retest reliability showing good result (Pearson's correlation, 0.695-0.905). Our data were amenable to factor analysis (KMO = 0.816, Bartlett's test, χ(45) = 1199.99, p < 0.001), and five factors above 1 were strongly loaded and clustered for each of the five factors. Comparing the scales preoperative to those 2 years postoperative, the average scores of the subscales all increased, and both the ES and SRM showing satisfied responsiveness. In external responsiveness analysis, the recovery rate a appeared to be most responsive to post-operative improvement.

Conclusions: The Simplified Chinese version of JOACMEQ was well-developed with great reliability and sensitive responsiveness. Our study demonstrated that JOACMEQ has content psychometric properties to identify postoperative improvements in CSM patients.
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http://dx.doi.org/10.1186/s12955-020-01602-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579864PMC
October 2020

sp. nov., a novel member of isolated from activated sludge collected in Hong Kong.

Int J Syst Evol Microbiol 2020 Nov;70(11):5799-5805

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.

A floc-forming bacterial strain, designated HKLI-1, was isolated from the activated sludge of a municipal sewage treatment plant in Hong Kong SAR, PR China. Cells of this strain were Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 18-37 °C (optimum, 28 °C), pH 5.5-9.0 (optimum, pH 7.5) and with 0-8.0 % (w/v) NaCl (optimum, 1-1.5 %) concentration. The major fatty acids of strain HKLI-1 were C and summed feature 3 (C ω7 and/or C ω6). The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified lipids. The DNA G+C content was 63.5 mol% from whole genomic sequence analysis. Based on the results of 16S rRNA gene sequences analysis, this strain should be assigned to the genus and is closely related to DQS-4 (94.93 % 16S rRNA gene sequence pairwise similarity), MF63 (94.91 %) and SWub3 (94.01 %), but separate from them by large distances in different phylogenetic trees. Based on whole genome analysis, the orthologous average nucleotide identity and DNA-DNA hybridization values against four of the closest relatives were 73.03-74.83 and 17.2-23.0 %, respectively. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrated that strain HKLI-1 could be distinguished from its phylogenetically related species, and that this strain represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HKLI-1 (= 72659=CCTCC AB 2019312).
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http://dx.doi.org/10.1099/ijsem.0.004476DOI Listing
November 2020

Prediction of Passive Torque on Human Shoulder Joint Based on BPANN.

Appl Bionics Biomech 2020 26;2020:8839791. Epub 2020 Aug 26.

Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, Tianjin University, Tianjin 300072, China.

In upper limb rehabilitation training by exploiting robotic devices, the qualitative or quantitative assessment of human active effort is conducive to altering the robot control parameters to offer the patients appropriate assistance, which is considered an effective rehabilitation strategy termed as assist-as-needed. Since active effort of a patient is changeable for the conscious or unconscious behavior, it is considered to be more feasible to determine the distributions of the passive resistance of the patient's joints versus the joint angle in advance, which can be adopted to assess the active behavior of patients combined with the measurement of robotic sensors. However, the overintensive measurements can impose a burden on patients. Accordingly, a prediction method of shoulder joint passive torque based on a Backpropagation neural network (BPANN) was proposed in the present study to expand the passive torque distribution of the shoulder joint of a patient with less measurement data. The experiments recruiting three adult male subjects were conducted, and the results revealed that the BPANN exhibits high prediction accurate for each direction shoulder passive torque. The results revealed that the BPANN can learn the nonlinear relationship between the passive torque and the position of the shoulder joint and can make an accurate prediction without the need to build a force distribution function in advance, making it possible to draw up an assist-as-needed strategy with high accuracy while reducing the measurement burden of patients and physiotherapists.
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http://dx.doi.org/10.1155/2020/8839791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474745PMC
August 2020

Revealing the Role of Liquid Metals at the Anode-Electrolyte Interface for All Solid-State Lithium-Ion Batteries.

ACS Appl Mater Interfaces 2020 Aug 15;12(34):38232-38240. Epub 2020 Aug 15.

Department of Materials Science, Fudan University, Shanghai 200433, China.

All-solid-state lithium-ion batteries (ASSLIBs) are receiving tremendous attention for safety concerns over liquid system. However, current ASSLIBs still suffer from poor cycling and rate performance because of unfavorable interfacial contact between solid electrolyte and electrodes, especially in the alloy-based anode. To wet the solid electrode/electrolyte interface, accommodate volume change, and further boost kinetics, liquid metal Ga is introduced into the representative Sb anode, and its corresponding role is comprehensively revealed by experimental results and theoretical calculations for the first time. In addition to interface contact and strain accommodation, with the aid of in situ generation of liquid metal Ga, the lithiation/de-lithiation activity of Sb is stimulated, showing outstanding rate and cycling performance in half cells. Furthermore, benefited from the in situ chemical reaction, TiS powder can be directly used to construct a novel "Li-free" TiS|LiBH|GaSb full cell, which exhibits an outstanding capacity retention of 226 mA h g after 1000 cycles at a current density of 0.5 A g. This work provides guidance for implementing future rational design of alloy anodes within ASSLIBs.
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http://dx.doi.org/10.1021/acsami.0c11001DOI Listing
August 2020

Production of Highly Active Extracellular Amylase and Cellulase From ZIM3 and a Recombinant Strain With a Potential Application in Tobacco Fermentation.

Front Microbiol 2020 21;11:1539. Epub 2020 Jul 21.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

In this study, a series of bacteria capable of degrading starch and cellulose were isolated from the aging flue-cured tobacco leaves. Remarkably, there was a thermophilic bacterium, ZIM3, that can simultaneously degrade both starch and cellulose at a wide range of temperature and pH values. Genome sequencing, comparative genomics analyses, and enzymatic activity assays showed that the ZIM3 strain expressed a variety of highly active plant biomass-degrading enzymes, such as the amylase AmyE1 and cellulase CelE1. The and PhoA-fusion assays indicated that these enzymes degrading complex plant biomass into fermentable sugars were secreted into ambient environment to function. Besides, the amylase and cellulase activities were further increased by three- to five-folds by using overexpression. Furthermore, a fermentation strategy was developed and the biodegradation efficiency of the starch and cellulose in the tobacco leaves were improved by 30-48%. These results reveal that ZIM3 and the recombinant strain exhibited high amylase and cellulase activities for efficient biodegradation of starch and cellulose in tobacco and could potentially be applied for industrial tobacco fermentation.
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http://dx.doi.org/10.3389/fmicb.2020.01539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385192PMC
July 2020

Quantitative analysis of the correlation between preoperative cervical degeneration and postoperative heterotopic ossification after cervical disc replacement: minimum 10-year follow-up data.

J Neurosurg Spine 2020 Jul 17:1-6. Epub 2020 Jul 17.

1Department of Orthopaedics, Peking University Third Hospital, Beijing, China; and.

Objective: The authors aimed to identify factors that may be useful for quantifying the amount of degenerative change in preoperative patients to identify ideal candidates for cervical disc replacement (CDR) in patients with a minimum of 10 years of follow-up data.

Methods: During the period from December 2003 to August 2008, 54 patients underwent CDR with a Bryan cervical disc prosthesis performed by the same group of surgeons, and all of the patients in this group with at least 10 years of follow-up data were enrolled in this retrospective analysis of cases. Postoperative bone formation was graded in radiographic images by using the McAfee classification for heterotopic ossification. Preoperative degeneration was evaluated in radiographs based on a quantitative scoring system. After univariate analysis, the authors performed multifactor logistic regression analysis to identify significant factors. To determine the cutoff points for the significant factors, a receiver operating characteristic (ROC) curve analysis was conducted.

Results: Study patients had a mean age of 43.6 years and an average follow-up period of 120.3 months. The patients as a group had a 68.2% overall incidence of bone formation. Based on univariate analysis results, data for patient sex, disc height, and the presence of anterior osteophytes and endplate sclerosis were included in the multivariate analysis. According to the analysis results, the identified independent risk factors for postoperative bone formation included disc height, the presence of anterior osteophytes, and endplate sclerosis, and according to a quantitative scoring system for degeneration of the cervical spine based on these variables, the ROC curve indicated that the optimal cutoff scores for these risk factors were 0.5, 1.5, and 1.5, respectively.

Conclusions: Among the patients who were followed up for at least 10 years after CDR, the incidence of postoperative bone formation was relatively high. The study results indicate that the degree of degeneration in the target level before surgery has a positive correlation with the incidence of postoperative ossification. Rigorous indication criteria for postoperative ossification should be applied in patients for whom CDR may be a treatment option.
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http://dx.doi.org/10.3171/2020.4.SPINE191303DOI Listing
July 2020

Enhancement of osteoblast cells osteogenic differentiation and bone regeneration by hydroxyapatite/phosphoester modified poly(amino acid).

Mater Sci Eng C Mater Biol Appl 2020 Jun 22;111:110769. Epub 2020 Feb 22.

College of Physics, Sichuan University, Chengdu, Sichuan 610065, China. Electronic address:

Hydroxyapatite/poly(amino acid) (HA/PAA) has been used to treat a variety of long bone and vertebral bony defects, and a further biocompatibility improvement is a key for better application. Phosphoester (PE) contained materials are highly biocompatible but could hardly treat massive bone defects due to its fast-degradation-derived mechanical instability. To address the problems of the two materials, we have incorporated PE molecule into the main chain of PAA by chemical bonding. As a result, the compressive strength of HA/PAA with 1 wt% and 2.5 wt% PE maintained in the range of 80-150 MPa after soaking in PBS for 12 weeks, which could be attributed to the amplified hydrogen-bonding inside composites. Besides, the PE-containing HA/PAAs with increased hydrophilic function groups (O=P-O bonds and O=P-N), created a more favourable surface for cell adhesion. Meanwhile, compared with HA/PAA, the PE-containing HA/PAAs had a fast minerlization speed and promoted cell osteogenic differentiation. Furthermore, the in vivo study indicated that PE-containing HA/PAAs could facilitate bone formation (4 weeks), and form a complete bone bridging (12 weeks) in a rabbit cranial bone defect. In summary, the HA/PE-m-PAAs possessed good mechanical stability, improved cytocompatibility and osteoconductivity, so the composites have a great potential for massive bone defect treatment.
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http://dx.doi.org/10.1016/j.msec.2020.110769DOI Listing
June 2020

Absorbable nanocomposites composed of mesoporous bioglass nanoparticles and polyelectrolyte complexes for surgical hemorrhage control.

Mater Sci Eng C Mater Biol Appl 2020 Apr 13;109:110556. Epub 2019 Dec 13.

Department of Orthopaedics, Zhongye Hospital, Shanghai 200941, China.

Absorbable polyelectrolyte complexes-based hemostats are promising for controlling hemorrhage in iatrogenic injuries during surgery, whereas their hemostatic efficacy and other performances require further improvement for clinical application. Herein, spherical mesoporous bioglass nanoparticles (mBGN) were fabricated, and mBGN-polyelectrolyte complexes (composed of carboxymethyl starch and chitosan oligosaccharide) nanocomposites (BGN/PEC) with different mBGN contents were prepared via in situ coprecipitation followed by lyophilization. The effect of various mBGN content (10 and 20 wt%) on morphology, zeta potential, water absorption, degradation behavior and ion release were systematically evaluated. The in vitro degradability was dramatically promoted and a more neutral environment was achieved with the incorporation of mBGN, which is preferable for surgical applications. The in vitro coagulation test with whole blood demonstrated that the incorporation of mBGN facilitated blood clotting process. The plasma coagulation evaluation indicated that BGN/PEC had increased capability to accelerate coagulation cascade via the intrinsic pathway than that of the PEC, while have inapparent influence on the extrinsic and common pathway. The in vivo hemostatic evaluation in a rabbit hepatic hemorrhage model revealed that BGN/PEC with 10 wt% mBGN (10BGN/PEC) treatment group had the lowest blood loss, although its hemostatic time is close to that of 20BGN/PEC treatment group. The cytocompatibility evaluation with MC3T3-L1 fibroblasts indicated that 10BGN/PEC induced a ~25% increase of cell viability compared to the PEC at days 4 and 7, indicating improved biocompatibility. These findings support the promising application of absorbable BGN/PEC with optimized mBGN content as internal hemostats and present a platform for further development of PEC-based hemostats.
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http://dx.doi.org/10.1016/j.msec.2019.110556DOI Listing
April 2020

Acute peripheral immune activation alters cytokine expression and glial activation in the early postnatal rat brain.

J Neuroinflammation 2019 Oct 31;16(1):200. Epub 2019 Oct 31.

MIND Institute, University of California, Davis School of Medicine, Sacramento, CA, 95817, USA.

Background: Neuroinflammation can modulate brain development; however, the influence of an acute peripheral immune challenge on neuroinflammatory responses in the early postnatal brain is not well characterized. To address this gap in knowledge, we evaluated the peripheral and central nervous system (CNS) immune responses to a mixed immune challenge in early postnatal rats of varying strains and sex.

Methods: On postnatal day 10 (P10), male and female Lewis and Brown Norway rats were injected intramuscularly with either a mix of bacterial and viral components in adjuvant, adjuvant-only, or saline. Immune responses were evaluated at 2 and 5 days post-challenge. Cytokine and chemokine levels were evaluated in serum and in multiple brain regions using a Luminex multiplex assay. Multi-factor ANOVAs were used to compare analyte levels across treatment groups within strain, sex, and day of sample collection. Numbers and activation status of astrocytes and microglia were also analyzed in the cortex and hippocampus by quantifying immunoreactivity for GFAP, IBA-1, and CD68 in fixed brain slices. Immunohistochemical data were analyzed using a mixed-model regression analysis.

Results: Acute peripheral immune challenge differentially altered cytokine and chemokine levels in the serum versus the brain. Within the brain, the cytokine and chemokine response varied between strains, sexes, and days post-challenge. Main findings included differences in T helper (Th) type cytokine responses in various brain regions, particularly the cortex, with respect to IL-4, IL-10, and IL-17 levels. Additionally, peripheral immune challenge altered GFAP and IBA-1 immunoreactivity in the brain in a strain- and sex-dependent manner.

Conclusions: These findings indicate that genetic background and sex influence the CNS response to an acute peripheral immune challenge during early postnatal development. Additionally, these data reinforce that the developmental time point during which the challenge occurs has a distinct effect on the activation of CNS-resident cells.
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http://dx.doi.org/10.1186/s12974-019-1569-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822372PMC
October 2019

Simonkolleite Coating on Poly(Amino Acids) to Improve Osteogenesis and Suppress Osteoclast Formation in Vitro.

Polymers (Basel) 2019 Sep 16;11(9). Epub 2019 Sep 16.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Zinc can enhance osteoblastic bone formation and stimulate osteogenic differentiation, suppress the differentiation of osteoclast precursor cells into osteoclasts, and inhibit pathogenic bacterial growth in a dose-dependent manner. In this study, simonkolleite, as a novel zinc resource, was coated on poly (amino acids) (PAA) via suspending PAA powder in different concentrations of zinc chloride (ZnCl) solution, and the simonkolleite-coated PAA (Zn-PAA) was characterized by SEM, XRD, FT-IR and XPS. Zinc ions were continuously released from the coating, and the release behavior was dependent on both the concentration of the ZnCl immersing solution and the type of soak solutions (SBF, PBS and DMEM). The Zn-PAA was cultured with mouse bone marrow stem cells (BMSCs) through Transwell plates, and the results indicated that the relative cell viability, alkaline phosphatase (ALP) activity and mineralization of BMSCs were significantly higher with Zn-PAA as compared to PAA. Moreover, the Zn-PAA was cultured with RAW264.7 cells, and the results suggested an inhibiting effect of Zn-PAA on the cell differentiation into osteoclasts. In addition, Zn-PAA exhibited an antibacterial activity against both and . These findings suggest that simonkolleite coating with certain contents could promote osteogenesis, suppress osteoclast formation and inhibit bacteria, indicating a novel way of enhancing the functionality of synthetic bone graft material and identifying the underline principles for designing zinc-containing bone grafts.
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http://dx.doi.org/10.3390/polym11091505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780185PMC
September 2019

Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins.

BMC Microbiol 2019 07 30;19(1):173. Epub 2019 Jul 30.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei Province, China.

Background: Most species of Shewanella harbor two ferrochelatase paralogues for the biosynthesis of c-type cytochromes, which are crucial for their respiratory versatility. In our previous study of the Shewanella loihica PV-4 strain, we found that the disruption of hemH1 but not hemH2 resulted in a significant accumulation of extracellular protoporphyrin IX (PPIX), but it is different in Shewanella oneidensis MR-1. Hence, the function and transcriptional regulation of two ferrochelatase genes, hemH1 and hemH2, are investigated in S. oneidensis MR-1.

Result: In the present study, deletion of either hemH1 or hemH2 in S. oneidensis MR-1 did not lead to overproduction of extracellular protoporphyrin IX (PPIX) as previously described in the hemH1 mutants of S. loihica PV-4. Moreover, supplement of exogenous hemins made it possible to generate the hemH1 and hemH2 double mutant in MR-1, but not in PV-4. Under aerobic condition, exogenous hemins were required for the growth of MR-1ΔhemH1ΔhemH2, which also overproduced extracellular PPIX. These results suggest that heme is essential for aerobic growth of Shewanella species and MR-1 could also uptake hemin for biosynthesis of essential cytochrome(s) and respiration. Besides, the exogenous hemin mediated CymA cytochrome maturation and the cellular KatB catalase activity. Both hemH paralogues were transcribed in wild-type MR-1, and the hemH2 transcription was remarkably up-regulated in MR-1ΔhemH1 mutant to compensate for the loss of hemH1. The periplasmic glutathione peroxidase gene pgpD, located in the same operon with hemH2, and a large gene cluster coding for iron, heme (hemin) uptake systems are absent in the PV-4 genome.

Conclusion: Our results indicate that the genetic divergence in gene content and gene expression between these Shewanella species, accounting for the phenotypic difference described here, might be due to their speciation and adaptation to the specific habitats (iron-rich deep-sea vent versus iron-poor freshwater) in which they evolved and the generated mutants could potentially be utilized for commercial production of PPIX.
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http://dx.doi.org/10.1186/s12866-019-1549-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664582PMC
July 2019

Persistent behavior deficits, neuroinflammation, and oxidative stress in a rat model of acute organophosphate intoxication.

Neurobiol Dis 2020 01 21;133:104431. Epub 2019 Mar 21.

Department of Molecular Biosciences, School of Veterinary Medicine, University of California-Davis, 1089 Veterinary Medicine Drive, Davis, CA 95616, USA; MIND Institute, School of Medicine, University of California-Davis, 2825 50th Street, Sacramento, CA 95817, USA. Electronic address:

Current medical countermeasures for organophosphate (OP)-induced status epilepticus (SE) are not effective in preventing long-term morbidity and there is an urgent need for improved therapies. Rat models of acute intoxication with the OP, diisopropylfluorophosphate (DFP), are increasingly being used to evaluate therapeutic candidates for efficacy in mitigating the long-term neurologic effects associated with OP-induced SE. Many of these therapeutic candidates target neuroinflammation and oxidative stress because of their implication in the pathogenesis of persistent neurologic deficits associated with OP-induced SE. Critical to these efforts is the rigorous characterization of the rat DFP model with respect to outcomes associated with acute OP intoxication in humans, which include long-term electroencephalographic, neurobehavioral, and neuropathologic effects, and their temporal relationship to neuroinflammation and oxidative stress. To address these needs, we examined a range of outcomes at later times post-exposure than have previously been reported for this model. Adult male Sprague-Dawley rats were given pyridostigmine bromide (0.1 mg/kg, im) 30 min prior to administration of DFP (4 mg/kg, sc), which was immediately followed by atropine sulfate (2 mg/kg, im) and pralidoxime (25 mg/kg, im). This exposure paradigm triggered robust electroencephalographic and behavioral seizures that rapidly progressed to SE lasting several hours in 90% of exposed animals. Animals that survived DFP-induced SE (~70%) exhibited spontaneous recurrent seizures and hyperreactive responses to tactile stimuli over the first 2 months post-exposure. Performance in the elevated plus maze, open field, and Pavlovian fear conditioning tests indicated that acute DFP intoxication reduced anxiety-like behavior and impaired learning and memory at 1 and 2 months post-exposure in the absence of effects on general locomotor behavior. Immunohistochemical analyses revealed significantly increased expression of biomarkers of reactive astrogliosis, microglial activation and oxidative stress in multiple brain regions at 1 and 2 months post-DFP, although there was significant spatiotemporal heterogeneity across these endpoints. Collectively, these data largely support the relevance of the rat model of acute DFP intoxication as a model for acute OP intoxication in the human, and support the hypothesis that neuroinflammation and/or oxidative stress represent potential therapeutic targets for mitigating the long-term neurologic sequelae of acute OP intoxication.
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http://dx.doi.org/10.1016/j.nbd.2019.03.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754818PMC
January 2020

A Muscle-Specific Rehabilitation Training Method Based on Muscle Activation and the Optimal Load Orientation Concept.

Appl Bionics Biomech 2018 22;2018:2365983. Epub 2018 Nov 22.

Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, Tianjin University, Tianjin 300072, China.

Training based on muscle-oriented repetitive movements has been shown to be beneficial for the improvement of movement abilities in human limbs in relation to fitness, athletic training, and rehabilitation training. In this paper, a muscle-specific rehabilitation training method based on the optimal load orientation concept (OLOC) was proposed for patients whose motor neurons are injured, but whose muscles and tendons are intact, to implement high-efficiency resistance training for the shoulder muscles, which is one of the most complex joints in the human body. A three-dimensional musculoskeletal model of the human shoulder was used to predict muscle forces experienced during shoulder movements, in which muscles that contributed to shoulder motion were divided into 31 muscle bundles, and the Hill model was used to characterize the force-length properties of the muscle. According to the musculoskeletal model, muscle activation was calculated to represent the muscle force. Thus, training based on OLOC was proposed by maximizing the activation of a specific muscle under each posture of the training process. The analysis indicated that the muscle-specific rehabilitation training method based on the OLOC significantly improved the training efficiency for specific muscles. The method could also be used for trajectory planning, load magnitude planning, and evaluation of training effects.
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http://dx.doi.org/10.1155/2018/2365983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282125PMC
November 2018

Temporal variations and potential sources of organophosphate esters in PM in Xinxiang, North China.

Chemosphere 2019 Jan 10;215:500-506. Epub 2018 Oct 10.

State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.

We monitored the concentrations of 10 organophosphate esters (OPEs) in 52 fine particulate matter (PM) samples in Xinxiang, Henan Province, North China, in 2015. During the sampling period, the OPE concentrations in most samples (n = 47) differed minimally and were relatively stable (mean: 2.02 ± 0.93 ng m), although several samples (n = 5) had high total OPE (ƩOPE) concentrations (mean: 9.99 ± 5.69 ng m), which may have been influenced by high PM levels. Meanwhile, some samples had high PM concentrations but low ƩOPE concentrations (i.e. low OPE/PM ratios) or low PM concentrations but high ƩOPE concentrations, which might have been influenced by air mass sources. Therefore, we assessed air mass sources using the Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT) model and wind direction frequency data, and subsequently analysed PM and OPE sources using a potential source contribution function (PSCF) model. The results revealed that air mass sources couldn't represent the source of specific pollutants, including PM and OPEs. Generally, both PM and OPEs were from Henan and Shandong Provinces; however, the major source areas differed, which may have resulted from diverse pollution characteristics in various source areas. The principal component analysis and PSCF results revealed that the 10 OPEs could be segmented into three groups, which were associated with different source areas. These results suggested that pollution characteristics of contaminants in source areas should be considered in source apportionment.
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http://dx.doi.org/10.1016/j.chemosphere.2018.10.063DOI Listing
January 2019

Both widespread PEP-CTERM proteins and exopolysaccharides are required for floc formation of Zoogloea resiniphila and other activated sludge bacteria.

Environ Microbiol 2018 05 26;20(5):1677-1692. Epub 2018 Mar 26.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Bacterial floc formation plays a central role in the activated sludge (AS) process, which has been widely utilized for sewage and wastewater treatment. The formation of AS flocs has long been known to require exopolysaccharide biosynthesis. This study demonstrates an additional requirement for a PEP-CTERM protein in Zoogloea resiniphila, a dominant AS bacterium harboring a large exopolysaccharide biosynthesis gene cluster. Two members of a wide-spread family of high copy number-per-genome PEP-CTERM genes, transcriptionally regulated by the RpoN sigma factor and accessory PrsK-PrsR two-component system and at least one of these, pepA, must be expressed for Zoogloea to build the floc structures that allow gravitational sludge settling and recycling. Without PrsK or PrsR, Zoogloea cells were planktonic rather than flocculated and secreted exopolysaccharides were released into the growth broth in soluble form. Overexpression of PepA could circumvent the requirement of rpoN, prsK and prsR for the floc-forming phenotype by fixing the exopolysaccharides to bacterial cells. However, overexpression of PepA, which underwent post-translational modifications, could not rescue the long-rod morphology of the rpoN mutant. Consistently, PEP-CTERM genes and exopolysaccharide biosynthesis gene cluster are present in the genome of the floc-forming Nitrospira comammox and Mitsuaria strain as well as many other AS bacteria.
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http://dx.doi.org/10.1111/1462-2920.14080DOI Listing
May 2018

Effects of alendronate on lumbar intervertebral disc degeneration with bone loss in ovariectomized rats.

Spine J 2017 07 10;17(7):995-1003. Epub 2017 Mar 10.

Department of Orthopaedic Surgery, The Affiliated Hospital of North China University of Science and Technology, 73 Jianshe Rd, Tangshan 063000, China. Electronic address:

Background Context: Osteoporosis adversely affects disc degeneration cascades, and prophylactic alendronate (ALN) helps delay intervertebral disc degeneration (IDD) in ovariectomized (OVX) rats. However, there remains no information regarding whether ALN affects IDD with bone loss.

Purpose: This study aimed to observe the effects of ALN on degenerative discs with bone loss induced by OVX in rats.

Study Design: This study used controlled in vivo experiments in rodents.

Methods: Thirty female Sprague-Dawley rats were randomly assigned to undergo sham surgery (n=10) or OVX surgery (n=20); 3 months later, the OVX animals were injected with either ALN (OVX+ALN, 15 µg/kg/2w, n=10) or normal saline (OVX+vehicle treatment [V], n=10). At 3 months after the ALN intervention, van Gieson staining and immunohistochemistry were used to investigate histologic and metabolic changes in the discs. Bone mineral density (BMD), micro-computed tomography, and biomechanical tests were conducted to determine the biological properties of the vertebrae.

Results: The OVX+ALN group exhibited significantly reduced morphologic degenerative alterations in both the nucleus pulposus and annulus fibrosus, with a markedly lower IDD score than that of the OVX+V group. The OVX+ALN samples showed increased disc height and decreased cartilage end plate thickness and bony area compared with the OVX+V group. Compared with saline, ALN administration markedly inhibited the type I collagen, matrix metalloprotease (MMP)-1, and MMP-13 expression levels while increasing the type II collagen and aggrecan expression levels in the disc matrix. Compared with the OVX+V group, OVX+ALN vertebrae revealed significantly enhanced BMD with increased biomechanical strength, as well as increased percent bone volume and trabecular thickness.

Conclusions: ALN has favorable effects on disc degeneration with bone loss and helps to alleviate IDD while enhancing the biological and mechanical properties of vertebrae and end plates.
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http://dx.doi.org/10.1016/j.spinee.2017.03.002DOI Listing
July 2017

The role of cities in reducing smoking in China.

Int J Environ Res Public Health 2014 Sep 26;11(10):10062-75. Epub 2014 Sep 26.

ThinkTank Research Center for Health Development, Tian Bao Yuan Liu Li, Beijing 100176, China.

China is the epicenter of the global tobacco epidemic. China grows more tobacco, produces more cigarettes, makes more profits from tobacco and has more smokers than any other nation in the world. Approximately one million smokers in China die annually from diseases caused by smoking, and this estimate is expected to reach over two million by 2020. China cities have a unique opportunity and role to play in leading the tobacco control charge from the "bottom up". The Emory Global Health Institute-China Tobacco Control Partnership supported 17 cities to establish tobacco control programs aimed at changing social norms for tobacco use. Program assessments showed the Tobacco Free Cities grantees' progress in establishing tobacco control policies and raising public awareness through policies, programs and education activities have varied from modest to substantial. Lessons learned included the need for training and tailored technical support to build staff capacity and the importance of government and organizational support for tobacco control. Tobacco control, particularly in China, is complex, but the potential for significant public health impact is unparalleled. Cities have a critical role to play in changing social norms of tobacco use, and may be the driving force for social norm change related to tobacco use in China.
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http://dx.doi.org/10.3390/ijerph111010062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210967PMC
September 2014

Challenges for philanthropy and tobacco control in China (1986-2012).

Tob Control 2013 Sep 25;22 Suppl 2:ii4-8. Epub 2013 May 25.

Global Health Institute, Emory University, Atlanta, Georgia 30322, USA.

Objective: To identify the international philanthropies that have invested in tobacco control in China, describe their role and strategies in changing the social norms of tobacco use, and define the outcomes achieved.

Methods: Information on the international philanthropic donor China projects, including activities and outcomes, was gathered from multiple sources including organisational websites, key informant interviews and emails with project officers, and published research papers and reports.

Results: Philanthropic donations to China's tobacco control efforts began in 1986. The donors provided funds to national, city, provincial government organisations, non-government organisations, universities, and healthcare organisations throughout China to establish a tobacco control workforce and effective programmes to reduce the burden of tobacco use.

Conclusions: International engagement has been an important dimension of tobacco control in China. Recognising the large burden of illness and capitalising on proven effective control measures, philanthropic organisations understandably seized the opportunity to achieve major health gains. Much of the international philanthropic investment has been directed at public information, policy change and building the Chinese research knowledge base. Documenting research and evaluation findings will continue to be important to ensure that promising practices and lessons learned are identified and shared with the China tobacco control practitioners. The ultimate question is whether foreign philanthropy is making a difference in tobacco control and changing social norms in China? The answer is plainly and simply that we do not know; the evidence is not yet available.
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http://dx.doi.org/10.1136/tobaccocontrol-2012-050924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756497PMC
September 2013

Expression of the transient receptor potential channels TRPV1, TRPA1 and TRPM8 in mouse trigeminal primary afferent neurons innervating the dura.

Mol Pain 2012 Sep 12;8:66. Epub 2012 Sep 12.

Washington University Pain Center and Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Background: Migraine and other headache disorders affect a large percentage of the population and cause debilitating pain. Activation and sensitization of the trigeminal primary afferent neurons innervating the dura and cerebral vessels is a crucial step in the "headache circuit". Many dural afferent neurons respond to algesic and inflammatory agents. Given the clear role of the transient receptor potential (TRP) family of channels in both sensing chemical stimulants and mediating inflammatory pain, we investigated the expression of TRP channels in dural afferent neurons.

Methods: We used two fluorescent tracers to retrogradely label dural afferent neurons in adult mice and quantified the abundance of peptidergic and non-peptidergic neuron populations using calcitonin gene-related peptide immunoreactivity (CGRP-ir) and isolectin B4 (IB4) binding as markers, respectively. Using immunohistochemistry, we compared the expression of TRPV1 and TRPA1 channels in dural afferent neurons with the expression in total trigeminal ganglion (TG) neurons. To examine the distribution of TRPM8 channels, we labeled dural afferent neurons in mice expressing farnesylated enhanced green fluorescent protein (EGFPf) from a TRPM8 locus. We used nearest-neighbor measurement to predict the spatial association between dural afferent neurons and neurons expressing TRPA1 or TRPM8 channels in the TG.

Results And Conclusions: We report that the size of dural afferent neurons is significantly larger than that of total TG neurons and facial skin afferents. Approximately 40% of dural afferent neurons exhibit IB4 binding. Surprisingly, the percentage of dural afferent neurons containing CGRP-ir is significantly lower than those of total TG neurons and facial skin afferents. Both TRPV1 and TRPA1 channels are expressed in dural afferent neurons. Furthermore, nearest-neighbor measurement indicates that TRPA1-expressing neurons are clustered around a subset of dural afferent neurons. Interestingly, TRPM8-expressing neurons are virtually absent in the dural afferent population, nor do these neurons cluster around dural afferent neurons. Taken together, our results suggest that TRPV1 and TRPA1 but not TRPM8 channels likely contribute to the excitation of dural afferent neurons and the subsequent activation of the headache circuit. These results provide an anatomical basis for understanding further the functional significance of TRP channels in headache pathophysiology.
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http://dx.doi.org/10.1186/1744-8069-8-66DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489865PMC
September 2012
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