Publications by authors named "Shuya Wang"

86 Publications

A new ionic liquid bridged periodic mesoporous organosilicas stationary phase for per aqueous liquid chromatography and its application in the detection of biogenic amines.

Talanta 2021 Dec 8;235:122795. Epub 2021 Aug 8.

Animal, Plant and Food Inspection Center, Nanjing Customs District PR China, Nanjing, 210000, China.

In order to solve the problems of using a large proportion of acetonitrile on the hydrophilic interaction liquid chromatography (HILIC) columns that was not environmentally friendly, and the poor acid and base resistance of traditional bonded silica columns, we reported a novel stationary phase of Au nanoparticles (Au NPs) covalently bonded to ionic liquid (ILs) bridged periodic mesoporous organosilicas (PMO) hydrophilic microspheres (PMO-ILs-Au NPs) for per aqueous liquid chromatography (PALC). The PMO hydrophilic microspheres were prepared by condensation of 1,3-bis(trimethoxysilylpropyl)imidazoliumchloride and 1, 2-Bis (triethoxysilyl) ethane and then modified with Au NPs the surface. The obtained materials were characterized by elemental analysis, FT-IR spectra, scanning electron microscope and transmission electron microscopy. The retention behavior was evaluated by investigating the effect of various chromatographic factors on the retention of different types of solutes. The retention mechanism of the stationary phases in PALC was a mixed type of anion-exchange and hydrophobic interaction. Compared with C-SiO column, the acid and base resistance of the stationary phase were greatly improved. Compared with the HILIC column and C column, some hydrophilic compounds such as six organic acids and eight biogenic amines were baseline separated with the enhanced resolution of the PMO-ILs-Au NPs column under the PALC mode. The efficiency of the new column was significantly higher than that of the HILIC column. Furthermore, the analysis of PALC-triple quadrupole mass spectrometry was developed for simultaneous detection of eight biogenic amines. This method could improve detection efficiency, save reagent and reduce environmental pollution. PALC as a green chromatography analytical method was suitable for the replacement of HILIC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2021.122795DOI Listing
December 2021

Spherical Nucleic Acid Vaccine Structure Markedly Influences Adaptive Immune Responses of Clinically Utilized Prostate Cancer Targets.

Adv Healthc Mater 2021 Sep 8:e2101262. Epub 2021 Sep 8.

Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.

Cancer vaccines, which activate the immune system against a target antigen, are attractive for prostate cancer, where multiple upregulated protein targets are identified. However, many clinical trials implementing peptides targeting these proteins have yielded suboptimal results. Using spherical nucleic acids (SNAs), we explore how precise architectural control of vaccine components can activate a robust antigen-specific immune response in comparison to clinical formulations of the same targets. The SNA vaccines incorporate peptides for human prostate-specific membrane antigen (PSMA) or T-cell receptor γ alternate reading frame protein (TARP) into an optimized architecture, resulting in high rates of immune activation and cytolytic ability in humanized mice and human peripheral blood mononuclear cells (hPBMCs). Specifically, administered SNAs elevate the production and secretion of cytokines and increase polyfunctional cytotoxic T cells and effector memory. Importantly, T cells raised from immunized mice potently kill targets, including clinically relevant cells expressing the whole PSMA protein. Treatment of hPBMCs increases costimulatory markers and cytolytically active T cells. This work demonstrates the importance of vaccine structure and its ability to reformulate and elevate clinical targets. Moreover, it encourages the field to reinvestigate ineffective peptide targets and repackage them into optimally structured vaccines to harness antigen potency and enhance clinical outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adhm.202101262DOI Listing
September 2021

Suppressing Interface Strain for Eliminating Double-Slope Behaviors: Towards Ideal Conformable Polymer Field-Effect Transistors.

Adv Mater 2021 Sep 4:e2101633. Epub 2021 Sep 4.

Center for Advanced Optoelectronic Functional Materials Research and Key Lab of UV-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, Changchun, 130024, China.

High-mobility polymer field-effect transistors (PFETs) are being actively explored for applications in soft electronic skin and low-cost flexible displays because of their superior solution processability, mechanical flexibility, and stretchability. However, most of high-mobility PFETs often deviate from the idealized behavior with variable mobility, large threshold voltage, and high off-state current, which masks their intrinsic properties and significantly impedes their practical applications. Here, it is first revealed that interface strain between polymer thin film and rigid substrate plays a crucial role in determining the ideality of PFETs, and demonstrate that various ideal conformable PFETs can be successfully fabricated by releasing strain. It is found that strain in film can be released by one-step peeling strategy, which can reduce π-π stacking distance and suppress generation of oxygen doped carriers, thereby obtaining linearly injected charge carriers and decreased carrier concentration in channel, eventually realizing ideal PFETs. More impressively, the fabricated ideal conformable PFET array displays outstanding conformability to curved objects, and meanwhile showing excellent organic light-emitting display driving capability. The work clarifies the effect of the interface strain on the device ideality, and strain can be effectively released by a facile peeling strategy, thus offering useful guidance for the construction of ideal conformable PFETs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202101633DOI Listing
September 2021

Phosphoglycerate mutase 2 is elevated in serum of patients with heart failure and correlates with the disease severity and patient's prognosis.

Open Med (Wars) 2021 11;16(1):1134-1142. Epub 2021 Aug 11.

Department of Cardiovascular Medicine, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, No. 105 Jiefang Road, Lixia District, Shandong Province, Jinan 250100, China.

Background: Heart failure (HF) is a serious and advanced stage of various cardiac diseases with high mortality and rehospitalization rates. Phosphoglycerate mutase 2 (PGAM2) overexpression was identified in the serum of patients with HF.

Material/methods: One hundred and fifty-three cases of HF were included in the present work. According to New York Heart Association (NYHA) classification, 22 were grade II, 84 were grade III, and 47 were grade IV. Serum PGAM2, NT-proBNP, B-type natriuretic peptide (BNP), troponin T (TNT), and Cys-C of HF patients were detected using ELISA assay. Left ventricular ejection fraction, left ventricular end-diastolic inner diameter, and left atrium (LA) inner diameter of the included cases were also detected by the cardiac color Doppler.

Results: The number of patients with atrial fibrillation was significantly higher in NYHA IV group than in groups II and III with statistical difference ( < 0.05). The serum PGAM2, NT-proBNP, and Cys-C were significantly higher in NYHA IV group than in NYHA II and NYHA III groups ( < 0.05). NT-proBNP had the highest prediction efficacy of HF severity and PGAM2 was also a potential biomarker for HF severity evaluation with relatively high sensitivity, specificity, and area under the ROC. The overall survival among NYHA II, III, and IV groups were statistically different ( = 0.04) with the median survival time of 25 months for NYHA III and IV groups.

Conclusion: PGAM2 is a new promising biomarker for evaluation of the severity of HF. Combination detection using multiple serum factors such as PGAM2, NT-proBNP, BNP, TNT, and Cys-C can improve the HF severity differential diagnosis performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/med-2021-0324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359905PMC
August 2021

Study of starch aging characteristics based on Terahertz technology.

Food Sci Nutr 2021 Aug 28;9(8):4431-4439. Epub 2021 Jun 28.

Shenzhen Institute of Terahertz Technology and Innovation Guangdong China.

Traditional methods for the determination of starch aging indicators often have a series of shortcomings such as time-consuming, high cost, large human error, damage to samples, environmental pollution, and high requirements for inspectors. Therefore, it is meaningful to find or establish a dynamic fingerprint identification pattern that can detect the aging degree of starch during the process of processing or storage quickly and accurately. It not only provides guidance for starch food processing but also saves a lot of human, material resources, and time. Terahertz technology is an emerging molecular spectroscopy technology in the 21st century. It is with low energy and basically harmless to the human body. It can also realize nondestructive testing of samples. In the experiment, the samples were prepared by the tableting method and the samples containing 20% of 50 mg samples were prepared with polyethylene as the diluent. The thickness of the samples was 1 mm and the diameter was 13 mm. The terahertz time-domain spectrometer was used to obtain the spectral information of aging starch at different aging times. After the pretreatment of the spectrum by vector normalization, first derivative, and multiple scattering correction, the prediction models of aging days, crystallinity, and resilience of aging starch were established, respectively. The determination coefficient ( ) of the established models is all greater than 95%, indicating that the established models are highly reliable and can be used to predict the aging days, crystallinity, and retrogradation degree of starch. And the of the prediction model based on the refractive index spectrum is greater than that of the absorption coefficient spectrum. The experimental method obtains the dynamic fingerprint identification map of starch in the aging process, realizes the real-time monitoring and detection of the starch aging process, and provides an effective means for the production and processing of starch-related industries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.2417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358343PMC
August 2021

Physiological Responses of Cucumber Seedlings to Different Supplemental Light Duration of Red and Blue LED.

Front Plant Sci 2021 12;12:709313. Epub 2021 Jul 12.

College of Horticulture, Gansu Agricultural University, Lanzhou, China.

Normal development of plants is inhibited by inadequate light in winter in greenhouses in Northwest China. Growth lamps, using light-emitting diodes (LEDs) with red blue light (7R2B), were used to supplement daylight for 1, 2, and 3 h. Seedling growth, photosynthesis, and photosynthetic product; the Calvin cycle key and sugar metabolism-related enzymes and their encoding genes; and the light signal sensing regulation of key gene expression were studied in greenhouse cucumbers under three treatments to determine the best supplemental light durations to enhance cucumber cultivation in greenhouses in winter. Treatment with LED red and blue light for 3 h significantly promoted the growth and development of cucumbers, root growth, and dry matter accumulation. It improved the photosynthetic rate, photosynthetic pigment content, and light energy utilization efficiency in cucumbers. Supplementation with red and blue LED light for 3 h upregulated the expression levels of key genes encoding the Calvin cycle and enzymes related to sugar metabolism in cucumber leaves, which promoted the synthesis and accumulation of photosynthates. The expression levels of phytochrome B, cryptochrome 1, and hypocotyl 5 in the cucumber leaves were also significantly upregulated after 3 h of light supplementation. Combined LED red and blue light for 3 h should be used to supplement natural light to enhance the cucumber cultivation in greenhouses in winter.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.709313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311605PMC
July 2021

Synergistic Immunostimulation through the Dual Activation of Toll-like Receptor 3/9 with Spherical Nucleic Acids.

ACS Nano 2021 Jul 19. Epub 2021 Jul 19.

Toll-like receptors (TLRs) are a family of proteins that modulate the innate immune system and control the initiation of downstream immune responses. Spherical nucleic acids (SNAs) designed to stimulate single members of the TLR family (, TLR7 or TLR9) have shown utility in cancer immunotherapy. We hypothesized that SNAs synthesized with multiple TLR agonists would enable the simultaneous activation of multiple TLR pathways for maximally synergistic immune activation. Here, we describe the synthesis of SNAs that incorporate both a TLR3 agonist (polyinosinic:polycytidylic acid, poly(I:C)) and TLR9 agonist (CpG oligonucleotide) on the same liposomal scaffold. In this design, CpG comprises the SNA oligonucleotide shell, and poly(I:C) is encapsulated in the liposome core. These dual-TLR activating SNAs efficiently codeliver high quantities of both agonists to the same target cell, yielding enhanced immunostimulation in various murine and human antigen-presenting cells (APCs). Moreover, codelivery of TLR agonists using the SNA both synchronizes and prolongs the duration of costimulatory molecule and major histocompatibility complex class II expression in APCs, which has been shown to be important for efficient downstream immune responses. Taken together, this SNA design provides a strategy for potently activating immune cells and increasing the efficiency of their activation, which likely will inform the preparation of nanomaterials for highly potent immunotherapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c03093DOI Listing
July 2021

Monitoring of Fluoride Content in Drinking Water by Ion Chromatography: A Case Study in the Suzhou Urban Area, China.

J AOAC Int 2021 Jun 30. Epub 2021 Jun 30.

Suzhou Foreign Language School, Suzhou215011, China.

Background: Both deficient and excessive intake of fluoride can lead to adverse health problems.

Objective: The aim of this study is to investigate the fluoride content in three types of drinking water in Suzhou urban area.

Method: Ion chromatography was employed to conduct the validation of analysis of fluoride ion in water and the method was applied to analyze the concentration of fluoride in 22 drinking water samples, including tap water, purified water, and commercially available bottled water, collected from five municipal districts of Suzhou urban area.

Results: The used method was validated in the range of 0.05 and 2.00 mg/L with good repeatability and accuracy. Results of water analysis indicated that fluoride content in tap water ranged from 0.267 to 0.336 mg/L (average 0.304 mg/L), and the levels of fluoride in purified water and bottled water were 0.068-0.317 mg/L (average 0.134 mg/L) and 0-0.120 mg/L (average 0.080 mg/L), respectively.

Conclusion: The amount of fluoride in all samples analyzed were lower than the limit set for fluoride in drinking water according to the China standard (1.0 mg/L). The low fluoride exposure for the population in the studied area was observed, and the replacement of tap water with purified and bottled water could further aggravate the deficient of fluoride intake for local residents.

Highlights: The present study carried out is the first to characterize the fluoride content in these three types of drinking water in Suzhou urban area by ion chromatography.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jaoacint/qsab081DOI Listing
June 2021

Nanotherapies for sepsis by regulating inflammatory signals and reactive oxygen and nitrogen species: New insight for treating COVID-19.

Redox Biol 2021 09 15;45:102046. Epub 2021 Jun 15.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410008, China; Hunan Provincial Key Laboratory of Cardiovascular Research, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410008, China. Electronic address:

SARS-CoV-2 has caused up to 127 million cases of COVID-19. Approximately 5% of COVID-19 patients develop severe illness, and approximately 40% of those with severe illness eventually die, corresponding to more than 2.78 million people. The pathological characteristics of COVID-19 resemble typical sepsis, and severe COVID-19 has been identified as viral sepsis. Progress in sepsis research is important for improving the clinical care of these patients. Recent advances in understanding the pathogenesis of sepsis have led to the view that an uncontrolled inflammatory response and oxidative stress are core factors. However, in the traditional treatment of sepsis, it is difficult to achieve a balance between the inflammation, pathogens (viruses, bacteria, and fungi), and patient tolerance, resulting in high mortality of patients with sepsis. In recent years, nanomaterials mediating reactive oxygen and nitrogen species (RONS) and the inflammatory response have shown previously unattainable therapeutic effects on sepsis. Despite these advantages, RONS and inflammatory response-based nanomaterials have yet to be extensively adopted as sepsis therapy. To the best of our knowledge, no review has yet discussed the pathogenesis of sepsis and the application of nanomaterials. To help bridge this gap, we discuss the pathogenesis of sepsis related to inflammation and the overproduction RONS, which activate pathogen-associated molecular pattern (PAMP)-pattern recognition receptor (PRR) and damage-associated molecular pattern (DAMP)-PRR signaling pathways. We also summarize the application of nanomaterials in the treatment of sepsis. As highlighted here, this strategy could synergistically improve the therapeutic efficacy against both RONS and inflammation in sepsis and may prolong survival. Current challenges and future developments for sepsis treatment are also summarized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.redox.2021.102046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205260PMC
September 2021

Survivin promotes rheumatoid arthritis fibroblast-like synoviocyte cell proliferation, and the expression of angiogenesis-related proteins by activating the NOTCH pathway.

Int J Rheum Dis 2021 Jul 7;24(7):922-929. Epub 2021 Jun 7.

Medical School, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, China.

Background: Survivin have been shown to play a crucial role in rheumatoid arthritis (RA); however, the regulatory mechanism of survivin in RA has not been fully elucidated. This study aims to investigate the effect of survivin on the proliferation and apoptosis of human RA fibroblast-like synoviocyte (RA-HFLS) cells in RA and its underlying mechanism through the NOTCH pathway.

Methods: The RA synovial tissues of 65 RA patients with partial resection of synovium of knee joint by arthroscopy were collected. The expression of survivin in synovial tissue was detected by immunohistochemistry, and the correlation of survivin expression and clinical-pathological parameters of patients was analyzed. In vitro, the proliferation of HFLS and RA-HFLS were detected by MTT; the apoptosis of HFLS and RA-HFLS were detected by flow cytometry; the expression of survivin proteins, key protein factors (Notch1, Jagged1, Hes1) in the Notch pathway, and angiogenesis-related proteins (vascular endothelial growth factor receptor 1 [VEGFR1], Ang1, Ang2) were determined by western blot.

Results: We found that survivin was highly expressed in RA synovial tissues and RA-HFLS cells, and was positively correlated with erythrocyte sedimentation rate, cyclic citrullinated peptide, C-reactive protein, Disease Activity Score of 28 joints and other pathological indexes. Knockdown survivin induces RA-HFLS cell apoptosis, suppresses proliferation and the expression of VEGFR1, Ang1, Ang2. In addition, blocking Notch pathway using FLI-06 significantly down-regulated survivin expression. When survivin is up-regulated, it promotes RA-HFLS cell proliferation, the expression of VEGFR1, Ang1, Ang2 and suppresses apoptosis by activating the NOTCH.

Conclusion: This study confirmed that survivin promotes RA-HFLS cell proliferation, the expression of angiogenesis-related proteins and suppresses apoptosis by activating the NOTCH pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1756-185X.14150DOI Listing
July 2021

Silencing the gene decreases the proliferation, migration and invasion of human triple-negative breast cancer cells.

Exp Ther Med 2021 Aug 24;22(2):792. Epub 2021 May 24.

Department of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.

Triple-negative breast cancer (TNBC) is highly invasive, has a high rate of recurrence and is associated with a poor clinical outcome when compared with non-TNBC due to a lack of effective and targeted treatments. The coatomer protein complex subunit β2 (COPB2) is upregulated in various types of malignant cancer. The present study demonstrated that COPB2 expression levels were significantly upregulated in breast carcinoma HS-578T cells (clonal cells originating from TNBC) when compared with non-TNBC MCF-7 cells. HS-578T cells also exhibited higher rates of proliferation, invasion and transendothelial migration when compared with MCF-7 cells. Moreover, it was identified that genetically silencing the gene using a lentivirus-short hairpin RNA inhibited the proliferative, colony formation, migratory and invasive properties of the TNBC HS-578T cells. Mediation of the silencing effect may be associated with regulating the phosphorylation of serine/threonine kinase AKT in the PI3K/AKT signaling pathway. These results suggested the importance of COPB2 in promoting the proliferation of TNBC cells and identified COPB2 as a potential novel therapeutic target.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170640PMC
August 2021

Exogenous stromal cell-derived factor-1 (SDF-1) suppresses the NLRP3 inflammasome and inhibits pyroptosis in synoviocytes from osteoarthritic joints via activation of the AMPK signaling pathway.

Inflammopharmacology 2021 Jun 3;29(3):695-704. Epub 2021 Jun 3.

Department of Rheumatology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng St, Harbin, 150001, China.

Objective: NLRP3 inflammasome may play a key role in OA pathogenesis. Stromal cell-derived factor-1 (SDF-1) is a homeostatic CXC chemokine. Since the role of SDF-1 in OA has not been explored, this study aimed to examine the effect of SDF-1 on NLRP3 inflammasome and pyroptosis in synoviocytes from OA joints.

Materials And Methods: Human synovium was obtained from OA patients for isolation of primary synoviocytes and a murine model of collagenase-induced OA was established for testing intra-articular injections of SDF-1. Immunoblotting assays were used to examine the effects and underlying mechanism of action of SDF-1 on NLRP3 inflammasome and synoviocyte pyroptosis in synoviocytes. Inhibitors of AMPK and PI3K-mTOR were utilized to investigate the key signaling pathways involved in SDF-1-mediated OA inflammasome formation and pyroptosis.

Results: Synoviocytes from OA joints exhibited significantly higher expression of NLRP3 inflammasome and biomarkers of synoviocyte pyroptosis relative to healthy individuals. This was confirmed in the collagenase-induced OA model, where OA synoviocytes had a significantly lower SDF-1 expression than healthy ones. SDF-1 treatment in synoviocytes of OA patients and collagenase-induced OA led to significant downregulation in the expression of NLRP3 inflammasome and synoviocyte pyroptosis biomarkers. Inhibition of the AMPK signaling pathway significantly suppressed the inhibitory effect of SDF-1 on NLRP3 inflammasome expression of OA synoviocytes. However, blocking the SDF-1-activated PI3K-mTOR signaling pathway could still suppress the expression of NLRP3 inflammasome and synoviocyte pyroptosis biomarkers.

Conclusions: SDF-1 ameliorates NLRP3 inflammasome and pyroptosis in OA synoviocytes through activation of the AMPK signaling pathway. Therefore, SDF-1 may be a novel therapeutic target for OA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10787-021-00814-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233244PMC
June 2021

High-Performance Full-Photolithographic Top-Contact Conformable Organic Transistors for Soft Electronics.

Adv Sci (Weinh) 2021 May 18;8(9):2004050. Epub 2021 Feb 18.

Center for Advanced Optoelectronic Functional Materials Research and Key Lab of UV-Emitting Materials and Technology of Ministry of Education Northeast Normal University 5268 Renmin Street Changchun 130024 China.

Organic thin-film transistors (OTFTs) are identified to be the most promising candidate for next-generation wearable and implantable electronics because of their unique advantages including their flexibility, low cost, long-term biocompatibility, and simple packaging. However, commercialization of organic transistors remains an enormous challenge due to their low mobility and lack of scalable strategy for high-precise soft devices. Here, a novel photolithography fabrication strategy is proposed, which is completely compatible with various commercial organic semiconductor materials, for the first demonstration of the fully photolithographic top-contact conformable OTFTs with the device density as high as 1523 transistors cm. Excellent electrical and mechanical properties with device yield as high as 100%, field-effect mobility up to 1-2 cm V s, and outstanding conformability are shown. This work provides a new strategy that can fully maximize the advantages of organic materials and photolithography technology, showing a great prospect in the development of high-performance, high-precise organic devices toward the commercialized and industrialized soft electronic products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202004050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097323PMC
May 2021

Posttraumatic growth in Chinese nurses and general public during the COVID-19 outbreak.

Psychol Health Med 2021 Mar 16:1-11. Epub 2021 Mar 16.

Department of Urology, The Changzhou Second People's Hospital Affiliated of Nanjing Medical University, Changzhou, China.

COVID-19 as a pandemic disease, till 18 May 2020, has infected more than 84,494 people in China and 4721,051 abroad. While countries around the world concentrate on controlling the pandemic to minimize damage to this country, the positive psychology brought to nurses and general public (GP) by COVID-19 should not be ignored. This study aims to assess post-traumatic growth (PTG) of Chinese nurses and GP during the COVID-19 pandemic. The study employed PTG in Chinese nurses and GP with Posttraumatic growth inventory questionnaire (PTGI) via a mobile app-based questionnaire, anxiety and ways to copy with stress in nurses were also employed. A total of 455 nurses and 424 GP were included in the statistical analysis. Results indicated that score of total PTGI and three dimensions, new possibilities, personal strength and spiritual change, were different between nurses and GP. Furthermore, score of total PTGI and all domains were significantly different between 178 front-line nurses (FLNs) and 277 non-front-line nurses (nFLNs). Importantly, sex, marriage status, professional titles, fertility, anxiety and ways to copy with stress were associated with PTG in nurses. Moreover, marriage status and ways to copy with stress were the predictors of PTG in nurses. Interestingly, this study found that WeChat network psychological counseling and phone app of application self-relaxation were good and effective coping strategies for nurses to relieve stress. Thus, the development of valid intervention programs for nurses to diminish job burnout and increase care quality was also important.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/13548506.2021.1897148DOI Listing
March 2021

Rapid progression of high-risk proliferative diabetic retinopathy induced by insulin intensive therapy: A case report.

Medicine (Baltimore) 2021 Feb;100(7):e24379

Department of Ophthalmology, Shandong University of Traditional Chinese Medicine.

Rationale: To summarize and analyze a case of rapid progression of high-risk proliferative diabetic retinopathy after the insulin intensive therapy (IT).

Patient Concerns: A 58-year-old type 2 diabetes female patient suffered a rapid and dramatic decline of vision acuity in the left eye in 2 months after the insulin IT. However, the best corrected visual acuity (BCVA) of her right eye, which was in much severer condition and received panretinal photocoagulation (PRP) before, improved after the IT.

Interventions: The patient received intravitreal injection of conbercept (IVC) to her left eye, and 5 days later, underwent pars plana vitrectomy (PPV), combined with PRP and silicon oil injection.

Outcomes: The postoperative BCVA of the left eye was 20/200 and improved to 20/160 one month later. During the subsequent 2 months of follow-up, her BCVA remained 20/160 in both eyes. Her blood glucose level also remained stable.

Lessons: Insulin IT for untreated proliferative diabetic retinopathy (PDR) patients can cause severe and irreversible consequences, so for such patients, the conservative treatment for glycemic control may be much safer. But if insulin IT is inevitable, the patient should undergo PRP promptly before the IT, and close eye monitoring during the IT is also essential.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000024379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899832PMC
February 2021

Blurred Electrode for Low Contact Resistance in Coplanar Organic Transistors.

ACS Nano 2021 Jan 18;15(1):1155-1166. Epub 2020 Dec 18.

Center for Advanced Optoelectronic Functional Materials Research, and Key Lab of UV-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, China.

Inefficient charge injection and transport across the electrode/semiconductor contact edge severely limits the device performance of coplanar organic thin-film transistors (OTFTs). To date, various approaches have been implemented to address the adverse contact problems of coplanar OTFTs. However, these approaches mainly focused on reducing the injection resistance and failed to effectively lower the access resistance. Here, we demonstrate a facile strategy by utilizing the blurring effect during the deposition of metal electrodes, to significantly reduce the access resistance. We find that the transition region formed by the blurring behavior can continuously tune the molecular packing and thin-film growth of organic semiconductors across the contact edge, as well as provide continuously distributed gap states for carrier tunnelling. Based on this versatile strategy, the fabricated dinaphtho[2,3-:2',3'-]thieno[3,2-]thiophene (DNTT) coplanar OTFT shows a high field-effect mobility of 6.08 cm V s and a low contact resistance of 2.32 kΩ cm, comparable to the staggered OTFTs fabricated simultaneously. Our work addresses the crucial impediments for further reducing the contact resistance in coplanar OTFTs, which represents a significant step of contact injection engineering in organic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.0c08122DOI Listing
January 2021

Cobweb-like, Ultrathin Porous Polymer Films for Ultrasensitive NO Detection.

ACS Appl Mater Interfaces 2020 Nov 10;12(47):52992-53002. Epub 2020 Nov 10.

Centre for Advanced Optoelectronic Functional Materials Research, and Key Laboratory of UV-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, China.

Gas sensors based on polymer field-effect transistors (FETs) have drawn much attention owing to the inherent merits of specific selectivity, low cost, and room temperature operation. Ultrathin (<10 nm) and porous polymer semiconductor films offer a golden opportunity for achieving high-performance gas sensors. However, wafer-scale fabrication of such high-quality polymer films is of great challenge and has rarely been realized before. Herein, the first demonstration of 4 in. wafer-scale, cobweb-like, and ultrathin porous polymer films is reported via a one-step phase-inversion process. This approach is extremely simple and universal for constructing various ultrathin porous polymer semiconductor films. Thanks to the abundant pores, ultrathin size, and high charge-transfer efficiency of the prepared polymer films, our gas sensors exhibit many superior advantages, including ultrahigh response (2.46 × 10%), low limit of detection (LOD) (<1 ppm), and excellent selectivity. Thus, the proposed fabrication strategy is exceptionally promising for mass manufacturing of low-cost high-performance polymer FET-based gas sensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c09821DOI Listing
November 2020

Increased Risk of Inflammatory Bowel Disease Among Patients With Ankylosing Spondylitis: A 13-Year Population-Based Cohort Study.

Front Immunol 2020 2;11:578732. Epub 2020 Oct 2.

Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

Aim: Ankylosing spondylitis (AS) primarily affects the axial skeleton and extraarticular structures. Small-scaled studies have reported that the incidence and prevalence of inflammatory bowel disease (IBD) are higher in patients with AS than in the general population. This study determined the incidence of IBD in patients with AS using a large scale population-based cohort dataset.

Methods: This was a retrospective cohort study. Patient data were collected from the Taiwan National Health Insurance Research Database from 2000 to 2012. We enrolled 3,804 patients with AS and 7,608 non-AS patients. The endpoint was IBD diagnosis by using International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) coding 555 and 556 after at least three outpatient visits or one hospital admission, until the end of 2012. The Kaplan-Meier performed to discriminate IBD Cox proportional hazard model was used to estimate the hazard ratio (HR) for IBD between the AS and non-AS groups.

Results: Among the population as a whole the Cox proportional hazard regression indicated that patients aged ≥65 years [adjusted HR (aHR): 2.48, 95% confidence interval (CI): 1.38-4.47] or with comorbidity of cancer (aHR: 3.51, 95% CI: 1.40-8.80) had a higher HR for IBD. Kaplan-Meier curves of cumulative incidence proportion of IBD indicated that patients with AS had a higher risk of IBD than the non-AS group in the subgroup aged <40 years (HR: 2.85, 95% CI: 1.51-5.40, p = 0.001).

Conclusions: Patients with AS aged <40 years had a higher IBD risk than did those without AS in Taiwan. Clinicians and patients should be aware of this association.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.578732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567031PMC
June 2021

Identification and Analysis of Genes Underlying Bone Mineral Density by Integrating Microarray Data of Osteoporosis.

Front Cell Dev Biol 2020 27;8:798. Epub 2020 Aug 27.

Department of Rheumatology and Immunology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Osteoporosis is a kind of brittle bone disease, which is characterized by a reduction in bone mineral density (BMD). In recent years, a number of genes and pathophysiological mechanisms have been identified for osteoporosis. However, the genes associated with BMD remain to be explored. Toward this end, we integrated multiple osteoporosis microarray datasets to identify and systematically characterize BMD-related genes. By integrating the differentially expressed genes from three osteoporosis microarray datasets, 152 genes show differentially expressed between high and low BMD osteoporosis samples in at least two of the three datasets. Among them, 88 were up-regulated in high BMD samples and 64 were up-regulated in low BMD samples. The expression of ZFP36, JUNB and TMEM8A were increased at high BMD samples in all three datasets. Hub genes were further identified by co-expression network analysis. Functional enrichment analysis showed that the gene up-regulated in high BMD were enriched in immune-related functions, suggesting that the immune system plays an important role in osteoporosis. Our study explored BMD-related genes based on the integration of osteoporosis microarray data, providing guidance to other researchers from a new perspective.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2020.00798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481435PMC
August 2020

Forensic characteristics and phylogenetic analyses of one branch of Tai-Kadai language-speaking Hainan Hlai (Ha Hlai) via 23 autosomal STRs included in the Huaxia Platinum System.

Mol Genet Genomic Med 2020 10 30;8(10):e1462. Epub 2020 Aug 30.

School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China.

Background: Hainan Island, located in the South China Sea and separated from the Leizhou Peninsula by Qiongzhou Strait, is the second largest island after Taiwan in China. With the expansion of Han Chinese and the gradual formation of "South Hlai and North Han", nowadays, Hainan Hlai is the second largest population after Han Chinese in Hainan Island. Ha Hlai, distributed in southwest and southern Hainan Island, is the dominant branch of Hlai and speaks Ha localism.

Methods: We utilized the Huaxia Platinum PCR Amplification System (including 23 autosomal STRs and 2 sex-linked markers) to obtain the first STR profiling batch of 657 Ha Hlai individuals (497 males and 160 females). In order to explore the genetic relationships between the studied Ha Hlai and other reference populations with different language families, population genetic analyses, including PCA, MDS, STRUCTURE, and phylogenetic analysis, were conducted based upon the raw data and allelic frequencies of the polymorphic autosomal STR markers.

Results: In total, 271 distinct alleles were observed at the 23 STR loci. The number of diverse alleles ranged from 7 at TPOX locus to 23 at FGA locus, and the allelic frequencies varied from 0.0008 to 0.5533. In addition, the CPE and CPD were 1-7.39 × 10 and 1-3.13 × 10 , respectively. The phylogenetic analyses indicated that Ha Hlai is a Tai-Kadai language-speaking and relatively isolated population which has a close genetic and geographical relationship with Hainan Hlai, and M95 is the dominant haplogroup in Ha Hlai (56.18%).

Conclusion: The 23 autosomal STR genetic markers were highly polymorphic as well as potentially useful for forensic applications in Hainan Ha Hlai population. The phylogenetic analyses demonstrated that small geographic scale gene flows could not be ignored and the shaping of the unique gene pool for each population was the combination effects of geographic, language, and cultural isolations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mgg3.1462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549582PMC
October 2020

A new approach for examining the neurovascular structure with phalloidin and calcitonin gene-related peptide in the rat cranial dura mater.

J Mol Histol 2020 Oct 13;51(5):541-548. Epub 2020 Aug 13.

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.

The neurovascular structures in the cranial dura mater have been studied with various histological techniques in the past years. In order to obtain a proper approach to reveal the detailed structures, different labeling methods for the cranial vessels and nerve fibers were tested in this study. Firstly, the labeling characteristics of phalloidin, alpha smooth muscle actin (α-SMA), and CD31 were compared in rat whole-mount cranial dura mater by using fluorescent immunohistochemistry or histochemistry. Secondly, according to their properties, phalloidin and α-SMA were selected to combine with calcitonin gene-related peptide (CGRP) to further demonstrate the cranial neurovascular structure. By these approaches, a three-dimensional map of blood vessels and nerve fibers within the whole-mount rat cranial dura mater was obtained. The results showed that phalloidin, α-SMA, and CD31 were preferably expressed in the wall of cranial vessels, corresponding to the arteriors, venules, and capillaries, respectively. Additionally, CGRP + nerve fibers were clearly demonstrated together with phalloidin + or α-SMA + vessels, forming a delicate neurovascular network in the cranial dura mater. The thick nerve bundles ran closely to the phalloidin + or α-SMA + vessels in parallel pattern, while the thin nerve fibers branched off from the bundles tending to surround the phalloidin + arterioles rather than α-SMA + venules. These findings suggest that phalloidin could be an appropriate biochemical maker to be effectively used together with CGRP for experiments examining the detailed spatial correlation of cranial blood vessels and nerve fibers in a three-dimensional view, which may provide clues for understanding the underlying mechanisms of cranial neurovascular disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10735-020-09903-7DOI Listing
October 2020

N-acyl--homoserine lactones release and microbial community changes in response to operation temperature in an anammox biofilm reactor.

Chemosphere 2021 Jan 10;262:127602. Epub 2020 Jul 10.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.

A 1 L lab-scale anaerobic ammonium oxidation (anammox) biofilm reactor with nitrogen loading rate of 0.11 g/L d was run for 110 days with the operation temperature declining from 36 °C to 15 °C. The total inorganic nitrogen removal efficiency showed a reduction from 80% to 66%, when the temperature declined from 36 °C to 15 °C. N-acyl--homoserine lactones (AHLs) concentrations, especially C8-HSL and C6-HSL, declined in both water and biomass phases, and this decline indicated that the quorum sensing weakened. Microbial community analysis revealed that Candidatus Kuenenia was the predominant anammox bacteria during the entire operating period. The abundance of Candidatus Kuenenia increased from 1.43% to 22.89% when the temperature decreasing from 36 °C to 15 °C. The correlation between microbial genus and AHLs was complicated. Overall, the temperature decrease weakened the quorum sensing so that the nitrogen removal performance deteriorated, and increasing the anammox activity might be an efficient way to improve performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.127602DOI Listing
January 2021

Uveal Effusion Syndrome as the Initial Manifestation of Primary Sjögren's Syndrome and Review of the Literature.

Ocul Immunol Inflamm 2020 Aug 4:1-7. Epub 2020 Aug 4.

Department of Ophthalmology, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China.

Purpose: The authors report a38-year-old woman with primary Sjögren's syndrome who initially showed recurrent blurred vision caused by uveal effusion syndrome and later developed dry mouth, dry eyes, and arthralgia. During the 5-year-course of disease, the patient's 3-time-onset was all manifested as blurred vision after decreased immunity. Despite the initial absence of sufficient immunological evidence, the final presence of positive serum anti-SS-A, rheumatoid factors, ANA, and inflammatory findings in minor salivary gland biopsy indicated primary Sjögren's syndrome.

Methods: Retrospective review of a case note.

Conclusions: The manifestation of UES requires further exploration of its real pathogenesis, and the possibility of systemic disease should never be excluded.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09273948.2020.1748202DOI Listing
August 2020

Development of Spherical Nucleic Acids for Prostate Cancer Immunotherapy.

Front Immunol 2020 8;11:1333. Epub 2020 Jul 8.

Division of Hematology/Oncology, Department of Medicine, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.

Although the strategy of therapeutic vaccination for the treatment of prostate cancer has advanced to and is available in the clinic (Sipuleucel-T), the efficacy of such therapy remains limited. Here, we develop Immunostimulatory Spherical Nucleic Acid (IS-SNA) nanostructures comprised of CpG oligonucleotides as adjuvant and prostate cancer peptide antigens, and evaluate their antitumor efficacy in syngeneic mouse models of prostate cancer. IS-SNAs with the specific structural feature of presenting both antigen and adjuvant CpG on the surface (hybridized model (HM) SNAs) induce stronger cytotoxic T lymphocyte (CTL) mediated antigen-specific killing of target cells than that for IS-SNAs with CpG on the surface and antigen encapsulated within the core (encapsulated model (EM) SNAs). Mechanistically, HM SNAs increase the co-delivery of CpG and antigen to dendritic cells over that for EM SNAs or admixtures of linear CpG and peptide, thereby improving cross-priming of antitumor CD8 T cells. As a result, vaccination with HM SNAs leads to more effective antitumor immune responses in two prostate cancer models. These data demonstrate the importance of the structural positioning of peptide antigens together with adjuvants within IS-SNAs to the efficacy of IS-SNA-based cancer immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.01333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362897PMC
April 2021

Effectiveness of gromwell (Lithospermum erythrorhizon) oil in the treatment of toxic epidermal necrolysis in a patient with dermatomyositis.

Dermatol Ther 2020 11 13;33(6):e14067. Epub 2020 Aug 13.

The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dth.14067DOI Listing
November 2020

Long non-coding RNA NORAD exhaustion represses prostate cancer progression through inhibiting TRIP13 expression via competitively binding to miR-495-3p.

Cancer Cell Int 2020 18;20:323. Epub 2020 Jul 18.

Department of Surgery, Huaihe Hospital, Henan University, No. 115, Ximen Street, Longting District, Kaifeng, 475000 Henan China.

Background: Prostate cancer (PCa) is a malignant heterogeneous tumor that threatens men's health. Long non-coding RNA activated by DNA damage (NORAD) and microRNA-495-3p (miR-495-3p) have been revealed to be concerned with the tumorigenesis and progression of diverse cancers. Nevertheless, the regulatory mechanism between NORAD and miR-495-3p in PCa is unclear.

Methods: The expression of NORAD, miR-495-3p, and thyroid hormone receptor interactor 13 (TRIP13) mRNA was detected with quantitative real-time polymerase chain reaction (qRT-PCR). The levels of Bcl-2, Bax, Cleaved-casp-3, TRIP13, cyclin D1, and PCNA were detected through western blot analysis. The proliferation, apoptosis, migration, and invasion of PCa cells were assessed through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), flow cytometry, or transwell assays. The relationship between NORAD or TRIP13 and miR-495-3p was confirmed via dual-luciferase reporter, RIP, or RNA pull-down assays.

Results: NORAD and TRIP13 were upregulated while miR-495-3p was downregulated in PCa tissues and cells. Both NORAD silencing and miR-495-3p upregulation accelerated cell apoptosis and curbed cell proliferation, migration, and invasion in PCa cells. Also, NORAD silencing repressed tumor growth in vivo. Notably, NORAD modulated TRIP13 expression by competitively binding to miR-495-3p. Furthermore, miR-495-3p repression reversed NORAD knockdown-mediated effects on the malignant behaviors of PCa cells. Moreover, TRIP13 enhancement overturned the effects of miR-495-3p overexpression on the proliferation, apoptosis, migration, and invasion of PCa cells.

Conclusion: NORAD depletion inhibited PCa advancement via the miR-495-3p/ TRIP13 axis, which provided a potential tactic for PCa treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-020-01371-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368683PMC
July 2020

In situ homogeneous formation of [email protected] for the rapid determination of formaldehyde residues by surface-enhanced Raman spectroscopy coupled with microhydrodistillation.

Mikrochim Acta 2020 05 28;187(6):353. Epub 2020 May 28.

Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.

The reaction of formaldehyde (HCHO) with the solution containing Au nanoparticles and [Ag(NH)] through Tollens' reaction is described to form [email protected] nanoparticles ([email protected]), which are used as a SERS enhancement material. A minimized hydrodistillation device with air condense is designed to obtain a clean aqueous background for SERS analysis and simplified pretreatment process. A good linear relationship is found between the SERS signals and the concentration of HCHO in the range 0.3 to 55 mg L when p-aminothiophenol (p-ATP) is selected as the Raman signal molecule, and the detection limit is 0.08 mg L. Stable SERS signals could be achieved due to both homogeneous formation of [email protected] and clean aqueous background. The relative standard deviation (RSD) of 15 batches samples is found to be 7.1%. The proposed approach has successfully been applied to the determination of HCHO residues in beer samples with low interferences. The recoveries of spiked samples are in the range 96 to 117% with RSDs lower than 5.9%. Graphical Abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-020-04332-5DOI Listing
May 2020

Long Non-Coding RNA OIP5-AS1 Knockdown Enhances CDDP Sensitivity in Osteosarcoma via miR-377-3p/FOSL2 Axis.

Authors:
Ling Liu Shuya Wang

Onco Targets Ther 2020 7;13:3853-3866. Epub 2020 May 7.

Department of Surgery, Huaihe Hospital of Henan University, Kaifeng 475000, Henan, People's Republic of China.

Background: Drug resistance is one of big obstacles for the treatment of tumor. Long non-coding RNA Opa-interacting protein 5-antisense RNA 1 (OIP5-AS1) was identified to involve in drug resistance. In this research, the effects of OIP5-AS1 on cisplatin (CDDP) resistance in osteosarcoma (OS) were mainly investigated.

Methods: The levels of OIP5-AS1, microRNA-377-3p (miR-377-3p), and FOS like 2 (FOSL2) were measured by quantitative real-time polymerase chain reaction. The inhibitory concentration 50 (IC) value of CDDP, cell viability and apoptotic rate was evaluated through Cell Counting Kit-8 and flow cytometry assays, respectively. The levels of multidrug resistance-associated protein 1 (MRP1), P-glycoprotein, B-cell lymphoma 2, Bcl2-associated X, cleaved-caspase-3, and FOSL2 were detected by Western blot assay. The interaction between miR-377-3p and OIP5-AS1 or FOSL2 was verified by Dual-Luciferase Reporter and RNA Immunoprecipitation assays. The function of OIP5-AS1 was detected by a xenograft tumor model in vivo.

Results: OIP5-AS1 and FOSL2 were up-regulated, while miR-377-3p was down-regulated in CDDP-resistant OS tissues and cells. OIP5-AS1 silencing inhibited cell viability and the IC value of CDDP, and promoted apoptotic rate in CDDP-resistant OS cells. Mechanically, OIP5-AS1 was verified as a sponge to miR-377-3p and FOSL2 was a target of miR-377-3p. Moreover, OIP5-AS1 knockdown repressed OS tumor growth and enhanced CDDP sensitivity of OS in vivo.

Conclusion: OIP5-AS1 positively modulated FOSL2 expression to decrease CDDP sensitivity in OS by sponging miR-377-3p.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S232918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7213903PMC
May 2020

Dual-Mode of Fluorescence Turn-On and Wavelength-Shift for Methylamine Gas Sensing Based on Space-Confined Growth of Methylammonium Lead Tribromide Perovskite Nanocrystals.

Anal Chem 2020 04 7;92(8):5661-5665. Epub 2020 Apr 7.

Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China.

The defect-tolerant nature of lead halide perovskites renders outstanding luminescence by simple space-confined growth in nanopores. The fluorescence turn-on and wavelength-shift phenomena could be found in the formation of methylammonium lead tribromide (MAPbBr) nanocrystals in hollow SiO nanospheres triggered by the reaction between methylamine (MA) gas and HPbBr/[email protected] nanospheres. The enhanced fluorescence intensity is linear with the MA concentration in the range of 1.0-95 ppm with a limit of detection (LOD) of 70 ppb (S/N = 3). In addition, the maximum emission wavelength is consistently red-shifted from 478.7 to 510.6 nm as the MA concentration increases from 1.0 to 95 ppm, imparting the potential for colorimetric sensing. By combining the fluorescence turn-on and colorimetric sensing modes, the flexible method meets the demands for visual discrimination and point-of-care determination with portable devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.0c00698DOI Listing
April 2020

Signaling Interaction between Facial and Meningeal Inputs of the Trigeminal System Mediates Peripheral Neurostimulation Analgesia in a Rat Model of Migraine.

Neuroscience 2020 05 12;433:184-199. Epub 2020 Mar 12.

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China. Electronic address:

Peripheral neurostimulation within the trigeminal nerve territory has been used for pain alleviation during migraine attacks, but the mechanistic basis of this non-invasive intervention is still poorly understood. In this study, we investigated the therapeutic role of peripheral stimulation of the trigeminal nerve, which provides homosegmental innervation to intracranial structures, by assessing analgesic effects in a nitroglycerin (NTG)-induced rat model of migraine. As a result of neurogenic inflammatory responses in the trigeminal nervous system, plasma protein extravasation was induced in facial skin by applying noxious stimulation to the dura mater. Noxious chemical stimulation of the dura mater led to protein extravasation in facial cutaneous tissues and caused mechanical sensitivity. Trigeminal ganglion (TG) neurons were double-labeled via retrograde tracing to detect bifurcated axons. Extracellular recordings of wide dynamic range (WDR) neurons in the spinal trigeminal nucleus caudalis (Sp5C) demonstrated the convergence and interaction of inputs from facial tissues and the dura mater. Peripheral neurostimulation of homotopic facial tissues represented segmental pain inhibition on cephalic cutaneous allodynia in the migraine model. The results indicated that facial territories and intracranial structures were directly connected with each other through bifurcated double-labeled neurons in the TG and through second-order WDR neurons. Homotopic stimulation at the C-fiber intensity threshold resulted in much stronger inhibition of analgesia than the same intensity of heterotopic stimulation. These results provide novel evidence for the neurological bases through which peripheral neurostimulation may be effective in treating migraine in clinical practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroscience.2020.03.004DOI Listing
May 2020
-->