Publications by authors named "Shuwen Li"

53 Publications

Melatonin influences the early growth stage in Zoysia japonica Steud. by regulating plant oxidation and genes of hormones.

Sci Rep 2021 Jun 11;11(1):12381. Epub 2021 Jun 11.

College of Grassland Science, Beijing Forestry University, Beijing, 100083, China.

Zoysia japonica is a commonly used turfgrass species around the world. Seed germination is a crucial stage in the plant life cycle and is particularly important for turf establishment and management. Experiments have confirmed that melatonin can be a potential regulator signal in seeds. To determine the effect of exogenous melatonin administration and explore the its potential in regulating seed growth, we studied the concentrations of several hormones and performed a transcriptome analysis of zoysia seeds after the application of melatonin. The total antioxidant capacity determination results showed that melatonin treatment could significantly improve the antioxidant capacity of zoysia seeds. The transcriptome analysis indicated that several of the regulatory pathways were involved in antioxidant activity and hormone activity. The hormones concentrations determination results showed that melatonin treatment contributed to decreased levels of cytokinin, abscisic acid and gibberellin in seeds, but had no significant effect on the secretion of auxin in early stages. Melatonin is able to affect the expression of IAA (indoleacetic acid) response genes. In addition, melatonin influences the other hormones by its synergy with other hormones. Transcriptome research in zoysia is helpful for understanding the regulation of melatonin and mechanisms underlying melatonin-mediated developmental processes in zoysia seeds.
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http://dx.doi.org/10.1038/s41598-021-91931-8DOI Listing
June 2021

[Impact and mechanism of NEMO binding domain peptide on pulmonary inflammation and apoptosis of lung tissues in mice with acute respiratory distress syndrome].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Apr;33(4):410-415

Guizhou Medical University, Guiyang 550004, Guizhou, China. Wang Yahui is working on the Department of Emergency, the People's Hospital of Weining County, Weining 553100, Guizhou, China. Corresponding author: Shen Feng, Email:

Objective: To investigate the effect of NEMO binding domain peptide (NBDP) on lung inflammation and apoptosis in mice with acute respiratory distress syndrome (ARDS) and its mechanism.

Methods: Thirty-six male BALB/c mice were divided into normal saline (NS) control group, ARDS model group, NBDP negative control group and 6, 12 and 18 μg NBDP pretreatment group by random number table method, with 6 mice in each group. ARDS mouse model was reproduced by aerosol inhalation lipopolysaccharide (LPS) 50 μL. An equivalent among of NS was inhaled in NS control group. The mice in NBDP negative control group were inhaled the materials similar to the non-functional NBDP 30 minutes before the aerosol inhalation LPS; 6, 12 and 18 μg of NBDP 50 μL were respectively inhaled in NBDP pretreatment groups. After inhalation of LPS for 6 hours, mice were sacrificed to get lung tissue and observe the degree of pathological injury and edema. Western blotting was used to detect the phosphorylation of nuclear factor-κB (NF-κB) pathway related proteins [NF-κB inhibitor (IκB) kinaseα/β(IKKα/β), IκBα and NF-κB p65; p-IKKα/β, p-IκBα, p-p65] and the expression of caspase-3 in lung tissue. The bronchoalveolar lavage fluid (BALF) was collected and the levels of inflammatory markers such as myeloperoxidase (MPO), interleukins (IL-1β, IL-8), and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA).

Results: ARDS model group had severe edema and hemorrhage, alveolar structure destruction, pulmonary hemorrhage and hyaline membrane formation etc. under light microscope, consistent with the pathological characteristics of ARDS lung tissue, suggesting that the ARDS model was successfully reproduced. ELISA showed that MPO, IL-1β, IL-8 and TNF-α levels of BALF in ARDS model group were obviously higher than those in NS control group. There were no significant differences in the above inflammatory indicators between NBDP negative control group and ARDS model group. The levels of MPO, IL-1β, IL-8 and TNF-α in NBDP pretreatment groups were significantly lower than those in ARDS model group in a dose-dependent manner, especially in 18 μg NBDP, the differences were statistically significant as compared with ARDS model group [MPO (ng/L): 393.32±19.35 vs. 985.87±101.50, IL-1β (ng/L): 43.05±5.11 vs. 97.68±10.88, IL-8 (ng/L): 84.64±2.32 vs. 204.00±17.37, TNF-α (ng/L): 229.13±17.03 vs. 546.73±62.72, all P < 0.05]. Western blotting showed that p-IKKα/β, p-IκBα, p-p65 and caspase-3 protein expressions in ARDS model group were significantly higher than those in NS control group. There was no significant difference in above NF-κB pathway and apoptosis-related protein expression between the NBDP negative control group and ARDS model group. The p-IKKα/β, p-IκBα, p-p65 and caspase-3 protein expression in NBDP pretreatment groups were significantly lower than those in ARDS model group in a dose-dependent manner, especially in 18 μg NBDP, the differences were statistically significant as compared with ARDS model group [p-IKKα/β protein (p-IKKα/β/β-actin): 0.15±0.02 vs. 0.42±0.04, p-IκBα protein (p-IκBα/β-actin): 0.10±0.01 vs. 0.93±0.30, p-p65 protein (p-p65/β-actin): 0.22±0.05 vs. 1.37±0.21, all P < 0.05].

Conclusions: NBDP can inhibit inflammatory response and apoptosis in ARDS lung tissue in a dose-dependent manner, and its mechanism is associated with interference NF-κB signaling pathway transduction.
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http://dx.doi.org/10.3760/cma.j.cn121430-20201106-00704DOI Listing
April 2021

Clinical competence and its association with self-efficacy and clinical learning environments among Chinese undergraduate nursing students.

Nurse Educ Pract 2021 Apr 16;53:103055. Epub 2021 Apr 16.

Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address:

Evaluation and cultivation of clinical competence in undergraduate nursing students are essential in nursing education. Self-efficacy and good clinical learning environments are expected to have a positive influence on the clinical competence of undergraduate nursing students, but few studies have focused on the relationships among clinical competence, self-efficacy, and clinical learning environments. This study aimed to examine clinical competence and its association with self-efficacy and clinical learning environments among Chinese undergraduate nursing students. A cross-sectional questionnaire survey was conducted with 1518 undergraduate nursing students being recruited from five medical colleges in China. The nursing students' clinical competence, self-efficacy, and clinical learning environments were assessed using a set of self-reported questionnaires, including Chinese versions of the Holistic Clinical Assessment Tool; General Self-Efficacy Scale; and Clinical Learning Environment, Supervision, and Nurse Teacher Evaluation Scale. Factors significantly associated with the clinical competence of undergraduate nursing students were professional interest, self-efficacy, and clinical learning environments, accounting for 36.1% of the total variance. Self-efficacy played a mediating role between clinical learning environments and clinical competence. The results of this study can be applied as reference for the enhancement of a nursing education program to improve the clinical competence of Chinese undergraduate nursing students.
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http://dx.doi.org/10.1016/j.nepr.2021.103055DOI Listing
April 2021

[Effects of andrographolide on the expression of procoagulant and fibrinolytic inhibition factors in rat type II alveolar epithelial cells stimulated by lipopolysaccharide].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Feb;33(2):155-160

Department of Critical Care Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China. Corresponding author: Shen Feng, Email:

Objective: To determine the effect of andrographolide (AD) on the expression of procoagulant and fibrinolytic inhibitory factors in rat type II alveolar epithelial cells (AEC II) stimulated by lipopolysaccharide (LPS).

Methods: The AEC II cells RLE-6TN in the logarithmic growth phase were divided into 5 groups: the normal control (NC) group, the LPS group, and the 6.25, 12.5, and 25 mg/L AD groups (AD 6.25 group, AD 12.5 group, AD 25 group). The NC group was cultured with RPMI 1640 conventional medium. In the LPS group, 5 mg/L LPS was added to the RPMI 1640 conventional medium for stimulation. Cells in the AD groups were treated with 6.25, 12.5, and 25 mg/L AD in advance for 1 hour and then given LPS to stimulate the culture. The cells and cell culture supernatant were collected 24 hours after LPS stimulation. The protein and mRNA expressions of tissue factor (TF), tissue factor pathway inhibitor (TFPI), and plasminogen activator inhibition-1 (PAI-1) in cells were detected by Western blotting and real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). The levels of procollagen III peptide (PIIIP), thrombin-antithrombin complex (TAT), antithrombin III (AT-III) and activated protein C (APC) in the cell supernatant were detected by enzyme linked immunosorbent assay (ELISA).

Results: Compared with the NC group, the protein and mRNA expressions of TF and PAI-1 in the LPS group were significantly increased, and the protein and mRNA expressions of TFPI were significantly reduced. At the same time, the levels of PIIIP and TAT in the cell supernatant were significantly increased, the levels of AT-III, APC were significantly reduced. Compared with the LPS group, the protein and mRNA expressions of TF and PAI-1 in AD 6.25 group, AD 12.5 group, AD 25 group were significantly reduced [TF/GAPDH: 0.86±0.08, 0.45±0.04, 0.44±0.04 vs. 1.32±0.10, TF mRNA (2): 2.59±0.25, 2.27±0.05, 1.95±0.04 vs. 4.60±0.26, PAI-1/GAPDH: 2.11±0.07, 1.45±0.04, 0.86±0.09 vs. 2.56±0.09, PAI-1 mRNA (2): 3.50±0.22, 2.23±0.29, 1.84±0.09 vs. 6.60±0.27, all P < 0.05], while the protein and mRNA expressions of TFPI were significantly increased [TFPI/GAPDH: 0.78±0.05, 0.81±0.03, 0.84±0.07 vs. 0.36±0.02, TFPI mRNA (2): 0.46±0.09, 0.69±0.07, 0.91±0.08 vs. 0.44±0.06, all P < 0.05]. Also the levels of PIIIP and TAT in the cell supernatant were significantly reduced, and the levels of AT-III and APC were significantly increased [PIIIP (μg/L): 13.59±0.23, 12.66±0.23, 10.59±0.30 vs. 15.82±0.29, TAT (ng/L): 211.57±6.41, 205.69±4.04, 200.56±9.85 vs. 288.67±9.84, AT-III (μg/L): 102.95±3.86, 123.92±2.63, 128.67±1.67 vs. 92.93±3.36, APC (μg/L): 1 188.95±14.99, 1 366.12±39.93, 1 451.15±29.69 vs. 1 145.55±21.07, all P < 0.05]. With the increase of the dose of AD, the above-mentioned promotion and inhibition effects became more obvious. In the AD 25 group, TF, PAI-1 protein and mRNA expressions decreased, TFPI mRNA expression increased, PIIIP level in the supernatant decreased and AT-III, APC levels increased compared with AD 6.25 group, the difference was statistically significant, and the decrease of PAI-1 protein expression and PIIIP level in the supernatant were also statistically significant compared with AD 12.5 group.

Conclusions: Andrographolide in the dose range of 6.25-25 mg/L can dose-dependently inhibit the expression and secretion of procoagulant and fibrinolytic inhibitor-related factors in AEC II cells RLE-6TN stimulated by LPS, and promote the secretion of anticoagulant factors. 25 mg/L has the most obvious effect.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200923-00647DOI Listing
February 2021

[Berberine dose-dependently inhibits the expression of procoagulant and fibrinolytic inhibitory factors in lipopolysaccharide-induced rat type II alveolar epithelial cells].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Jan;33(1):53-58

Department of Critical Care Medicine, Guizhou Medical University Affiliated Hospital, Guiyang 550004, Guizhou, China. Corresponding author: Shen Feng, Email:

Objective: To observe the effects of berberine on procoagulant and fibrinolytic inhibitory factors produced by rat type II alveolar epithelial cell (AEC II) induced by lipopolysaccharide (LPS).

Methods: AEC II cells (RLE-6TN cells) were cultured in vitro, and the cells in logarithmic growth phase were collected. The cytotoxicity text of berberine was detected by cell counting kit-8 (CCK-8) to determine the drug concentration range according to inhibition concentration of half cells (IC). The RLE-6TN cells were divided into five groups, the cells in blank control group were cultured in DMEM; the cells in LPS group were stimulated with 5 mg/L LPS; and the cells in berberine pretreatment groups were pretreated with 20, 50 and 80 μmol/L berberine for 1 hour, and then were co-cultured with 5 mg/L LPS. The cells were collected after LPS induced for 24 hours. The protein and mRNA expression levels of tissue factor (TF), tissue factor pathway inhibitor (TFPI) and plasminogen activator inhibitor-1 (PAI-1) in the cells were detected by Western blotting and real-time fluorescence quantification reverse transcription-polymerase chain reaction (RT-qPCR). The levels of activated protein C (APC), precollagen III peptide (PIIIP), thrombin-antithrombin complex (TAT) and antithrombin III (AT III) in the cell supernatant were measured by enzyme linked immunosorbent assay (ELISA).

Results: According to the inhibition rate curve, the IC of berberine on RLE-6TN cells was 81.16 μmol/L. Therefore, 20, 50 and 80 μmol/L were selected as the intervention concentration of berberine. Compared with the blank control group, the expression and secretion of procoagulant and fibrinolytic inhibitory factors were abnormal in RLE-6TN cells after LPS induced for 24 hours. The protein and mRNA expression levels of TF and PAI-1 in the LPS group were significantly increased, but the protein and mRNA expression levels of TFPI were significantly decreased. Meanwhile, the levels of APC and AT III in the cell supernatant were significantly decreased, while the levels of PIIIP and TAT were significantly increased. After pretreatment with berberine, the abnormal expression and secretion of procoagulant and fibrinolytic inhibitory factors induced by LPS were corrected in a dose-dependent manner, especially in 80 μmol/L. Compared with the LPS group, the protein and mRNA expression levels of TF and PAI-1 in the berberine 80 μmol/L group were significantly decreased [TF protein (TF/GAPDH): 0.45±0.02 vs. 0.55±0.03, TF mRNA (2): 0.39±0.08 vs. 1.48±0.11, PAI-1 protein (PAI-1/GAPDH): 0.37±0.02 vs. 0.64±0.04, PAI-1 mRNA (2): 1.14±0.29 vs. 4.18±0.44, all P < 0.01] and those of TFPI were significantly increased [TFPI protein (TFPI/GAPDH): 0.53±0.02 vs. 0.45±0.02, TFPI mRNA (2): 0.94±0.08 vs. 0.40±0.05, both P < 0.01]. Meanwhile, the levels of APC and AT III in the cell supernatant were significantly increased [APC (μg/L): 1 358.5±26.0 vs. 994.2±23.1, AT III (μg/L): 118.0±7.4 vs. 84.4±2.7, both P < 0.01], while those of PIIIP and TAT were significantly decreased [PIIIP (μg/L): 11.2±0.4 vs. 18.6±0.9, TAT (ng/L): 222.1±2.8 vs. 287.6±7.0, both P < 0.01].

Conclusions: Berberine could inhibit the LPS-induced expressions of procoagulant and fibrinolytic inhibitory factors in rat AEC II cells and promote the expressions of anticoagulant factors in a dose-dependent manner. Berberine may be a new therapeutic target for alveolar hypercoagulability and fibrinolysis inhibition in acute respiratory distress syndrome (ARDS).
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http://dx.doi.org/10.3760/cma.j.cn121430-20200917-00636DOI Listing
January 2021

[Meta-analysis of the impact of continuous anticoagulation therapy with low-dose heparin or low molecular weight heparin on the prognosis of patients with acute respiratory distress syndrome].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Dec;32(12):1479-1486

Department of Critical Care Medicine, Guizhou Medical University Affiliated Hospital, Guiyang 550004, Guizhou, China. Corresponding author: Shen Feng, Email:

Objective: The effects of low-dose heparin and low molecular weight heparin on the efficacy and prognosis of patients with acute respiratory distress syndrome (ARDS) were systematically evaluated.

Methods: Chinese and English databases such as Wanfang data, VIP database, China National Knowledge Infrastructure (CNKI), China Biomedical Literature Service system (SinoMed), American National Medical Library database (PubMed), Netherlands Medical Abstracts database (Embase) and Cochrane library database were searched for the randomized controlled trials (RCTs) of heparin or low molecular weight heparin in the treatment of ARDS. The search time is from July 1999 to June 2020. The control group was given routine treatment, while the experimental group was given heparin 5-10 U×kg×h, or low molecular heparin subcutaneous injected 2 500-5 000 U once every 12 hours for 7 days on the basis of the routine treatment. The main outcome were 28-day mortality, arterial oxygen saturation (SaO), acute physiology and chronic health evaluation II (APACHE II), length of stay in (intensive care unit) ICU and mechanical ventilation time, and the secondary outcome indexes were C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), lung injury score, respiratory frequency, activated partial thromboplastin time (APTT) and prothrombin time (PT) after 7 days' treatment. The two researchers collected data and evaluated the quality of the literature according to the Cochrane 5.1. The stability of the Meta-analysis results was tested by sensitivity analysis, and the publication bias was included in the funnel chart analysis.

Results: Fourteen Chinese literatures were included, with a total of 894 cases, including 454 cases in the experimental group and 440 cases in the control group. The results of Meta-analysis showed that the 28-day fatality rate in the experimental group was significantly lower than that in the control group [relative risk (RR) = 0.54, 95% confidence interval (95%CI) was 0.38-0.76, P = 0.000 4], and 7-day SaO significantly increased [heparin: mean difference (MD) = 48.27, 95%CI was 29.95-66.59, P < 0.000 01]. Low molecular weight heparin: MD = 56.67, 95%CI was 41.22-71.13, P < 0.000 01]. Compared with the control group, the APACHE II score (MD = -4.28, 95%CI was -5.15 to -3.42, P < 0.000 01), the lung injury score (MD = -1.19, 95%CI was -1.35 to -1.03, P < 0.000 01) and the respiratory rate (MD = -4.76, 95%CI was -6.26 to -3.26, P < 0.000 01) in the experimental group were significantly lower than those in the control group after 7 days' treatment. The time of staying in ICU (MD = -4.85, 95%CI was -6.94 to -2.76, P < 0.000 01) and the time of mechanical ventilation (MD = -2.93, 95%CI was -3.34 to -2.52, P < 0.000 01) in the experimental group were lower than those in the control group. After 7 days' treatment, the levels of IL-6 (MD = -38.50, 95%CI was -59.01 to -17.99, P < 0.000 01), TNF-α (MD = -16.24, 95%CI was -31.11 to -1.38, P < 0.000 01) and CRP (MD = -5.5, 95%CI was -6.47 to -4.27, P < 0.000 01) were significantly lower in the experimental group than those in the control group. However, after 7 days' treatment, there were no significant differences in APTT (MD = -0.55, 95%CI was -1.61 to 0.51, P = 0.27) or PT (MD = -0.41, 95%CI was -1.48 to 0.66, P = 0.45). For the indicators with high heterogeneity, Meta-analysis was carried out again by excluding any study, and the overall effect value did not change significantly, suggesting that the results were relatively robust. Funnel chart analysis was carried out on the indexes with more than 10 articles included in the literature. The results showed that the literature had publication bias, but the bias was small.

Conclusions: Continuous anticoagulation therapy with low dose heparin and low molecular weight heparin can improve the prognosis and reduce the mortality of patients with ARDS.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200928-00652DOI Listing
December 2020

Calcineurin inhibitors in the treatment of primary focal segmental glomerulosclerosis: A protocol of systematic review and meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2021 Jan;100(4):e24533

Gansu University of Chinese Medicine, Lanzhou, China.

Background: Evidence suggesting a role for including calcineurin inhibitors(CNIs) in early therapy remains limited for low quality and mainly based on small observation cohort study. We will conduct a systematic reviews to explore the effect and adverse effect of calcineurin inhibitors compared with other interventions in the treatment of primary focal segmental glomerulosclerosis (FSGS).

Methods: A comprehensive literature search of MEDLINE (through PubMed), EMBASE, The Cochrane Library, Cochrane Central Register of Controlled Trials (CENTRAL) will be conducted. Two investigators will independently select studies, extract data and assess the quality of the included study. Extracted information will include study characteristics, the contents of included randomized controlled trials, outcomes, the quality of randomized controlled trials and etc. A risk of bias tool will be used to assess the methodological quality. Any disagreement will be resolved by the third investigator. There is no requirement of ethical approval and informed consent.

Results: This study will provide high-quality evidence for treatment of FSGS in terms of effectiveness and safety.

Conclusion: This systematic review aims to provide evidence for treatment of FSGS in different CNIs.

Registration: The systematic review and meta-analysis is registered in the OSF REGISTERS (10.17605/OSF.IO/3B7DE) international prospective register of systematic review.
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http://dx.doi.org/10.1097/MD.0000000000024533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850689PMC
January 2021

Metal-organic interface engineering for coupling palladium nanocrystals over functionalized graphene as an advanced electrocatalyst of methanol and ethanol oxidation.

J Colloid Interface Sci 2021 Apr 25;588:384-392. Epub 2020 Dec 25.

Inner Mongolia Key Laboratory of Photoelectric Functional Materials, College of Chemistry and Life Sciences, Chifeng University, Chifeng 024000, China.

Adjusting the surface structures and electronic structures of metal nanocrystals (NCs) by the metal-organic interface interaction is an emerging strategy to enhance their electrocatalytic behavior. In this work, the d-phenylalanine-functionalized graphene (DPHE-GS) anchoring Pd NCs (denoted as Pd/DPHE-GS) was fabricated via the diazo-reaction followed by a simple chemical reduction. Owing to the metal-organic interface interaction between Pd NCs and DPHE, the size, distribution and electronic structures of Pd NCs on the surface of DPHE-GS can be adjusted. Therefore, the Pd/DPHE-GS shows the highest electrocatalytic activity and the most robust long-term durability and stability towards methanol and ethanol oxidation reaction (MOR and EOR) compared to the commercial Pd/C and other counterparts. This work presents an effective interface engineering strategy to enhance electrocatalytic property.
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http://dx.doi.org/10.1016/j.jcis.2020.12.080DOI Listing
April 2021

The effect of secondary capping on the control of phosphorus release from sediment by activated thin-layer capping with Al-PIA.

Environ Sci Pollut Res Int 2021 Apr 6;28(14):18062-18069. Epub 2021 Jan 6.

College of Civil Engineering, Huaqiao University, Jimei Avenue 668, Jimei District, Xiamen, 361021, China.

It is well-known that the activated thin-layer capping covering by secondary capping of contaminated sediment poses a threat to the inactivation of activated material. In this study, the static simulation experiment was conducted to study the effect of secondary capping thickness by sediment on the control of TP release from the sediment by aluminum-based P-inactivation agent (Al-PIA), and to propose the phosphorus adsorption pathway of Al-PIA. The results showed that Al-PIA could effectively reduce the release of phosphorus pollutants from the sediment at the capping intensity of 2 kg/m. When the secondary capping thickness of sediment were 0, 2, 4, 7, 10, and 15 mm, the average removal rates of TP were 87.57%, 76.39%, 61.22%, 51.32%, 41.93%, and 32.11%, respectively, indicating that the removal efficiency of phosphorus decreased with the increase of the secondary capping thickness of the sediment. The adsorbed phosphorus by Al-PIA was mainly non-apatite inorganic phosphorus (NAIP) in inorganic phosphorus. With the increase of the secondary capping thickness of sediment, the NAIP proportion of phosphorus adsorbed by Al-PIA increased. Meanwhile, the removal rate of phosphorus in the activated capping system showed a first increase and then decrease trend, and the removal rates of total phosphorus (TP), inorganic phosphorus (IP), and organic phosphorus (OP) were obvious except for that of organic phosphorus (OP).
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http://dx.doi.org/10.1007/s11356-020-11828-3DOI Listing
April 2021

The discovery of quinoline derivatives, as NF-κB inducing kinase (NIK) inhibitors with anti-inflammatory effects in vitro, low toxicities against T cell growth.

Bioorg Med Chem 2021 01 7;29:115856. Epub 2020 Nov 7.

School of Sciences, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, PR China. Electronic address:

NIK is a critical regulatory protein of the non-classical NF-kB pathway, and its dysregulated activation has been proved to be one of the pathogenic factors in a variety of autoimmune diseases and inflammatory diseases. Nevertheless, its corresponding development of inhibitors faces many obstacles, including the lack of structure types of known inhibitors, immature activity evaluation methods of compounds in vitro. In this study, a series of quinoline derivatives were obtained through rational design and chemical synthesis. Among them, the representative compounds 17c and 24c have excellent inhibitory activities on LPS-induced macrophage (J774) nitric oxide release and anti-Con A-stimulated primary T cell proliferation. This evaluation method has good universality and overcomes the obstacles mentioned above, which are faced by the current inhibitor research to a certain extent. Besides, the compound's toxicity against the growth of T cells under non-stress conditions was evaluated, for the first time, as an indicator for the investigation to avoid potential safety risks. Pharmacokinetic properties evaluation of the less toxic compound 24c confirmed its good metabolic behavior (especially oral properties, F% = 21.7%), and subsequent development value.
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http://dx.doi.org/10.1016/j.bmc.2020.115856DOI Listing
January 2021

Emodin improves alveolar hypercoagulation and inhibits pulmonary inflammation in LPS-provoked ARDS in mice via NF-κB inactivation.

Int Immunopharmacol 2020 Nov 21;88:107020. Epub 2020 Sep 21.

Department of Intensive Care Unit, Guizhou Medical University Affiliated Hospital, Guiyang 550001, China. Electronic address:

Background: Alveolar hypercoagulation and pulmonary inflammation are important characteristics and they regulate each other in acute respiratory distress syndrome (ARDS). NF-κB pathway has been confirmed to be involved in regulation of this crosstalk. Emodin, a traditional Chinese herb, shows potent inhibitory effect on NF-κB pathway, but whether it is effective in alveolar hypercoagulation and pulmonary inflammation in ARDS remains to be elucidated.

Purpose: The aim of this experiment was to evaluate the efficacy of emodin on LPS-provoked alveolar hypercoagulation and excessive pulmonary inflammation in ARDS, and its potential mechanism.

Methods: Mice ARDS was set up through LPS (40 μl, 4 mg/ml) inhalation. Male mice were randomly received with BPS, LPS only, LPS+ emodin (5 mg/kg, 10 mg/kg, 20 mg/kg, respectively) and BAY65-1942, an inhibitor of IKKβ. After 48 h of LPS stimulation, pulmonary pathological injury, expressions of Tissue factor (TF), plasminogen activator inhibitor (PAI)-1, activated protein C (APC), collagen Ⅰ, collagen III, interleukin (IL) 8, IL-1β and tumor necrosis factor (TNF)-α in lung tissues, as well as concentrations of antithrombin III (AT III), procollagen peptide type III (PIIIP), soluble thrombomodulin (sTM), thrombin antithrombin complex (TAT), myeloperoxidase (MPO) and the percentage of inflammatory cells in bronchoalveolar lavage fluid (BALF) were all determined. NF-κB pathway activation as well as NF-κB DNA binding activity in pulmonary tissue were simultaneously checked.

Results: LPS stimulation resulted in obvious lung injury, excessive inflammatory cells infiltration, which all were dose-dependently ameliorated by emodin. Expressions of TF, PAI-1, collagen Ⅰ and collagen III as well as IL-8, IL-1β and TNF-α in pulmonary tissue were all elevated while APC decreased under LPS provocation, which were all reversed by emodin treatment in dose-dependent manner. LPS promoted the secretions of PIIIP, sTM, TAT and inhibited AT III production in BALF, and resulted in high levels of MPO and the percentage of inflammatory cells in BALF, all of which were significantly and dose-dependently attenuated while AT III production was increased by emodin. Meanwhile, emodin effectively inhibited NF-κB pathway activation and attenuated p65 DNA binding activity induced by LPS inhalation. Emodin and BAY-65-1942 had similar impacts in this experiment.

Conclusions: Emodin improves alveolar hypercoagulation and fibrinolytic inhibition and depresses excessive pulmonary inflammation in ARDS mice in dose-dependent manner via NF-κB inactivation. Our data demonstrate that emodin is expected to be an effective drug in alveolar hypercoagulation and pulmonary inflammation in ARDS.
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http://dx.doi.org/10.1016/j.intimp.2020.107020DOI Listing
November 2020

The caring experience and supportive care needs of male partners for women with gynaecologic cancer: A qualitative literature review.

J Clin Nurs 2020 Dec 19;29(23-24):4469-4481. Epub 2020 Oct 19.

Alice Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Objective: To systematically review male partners' caring experience and supportive care needs when caring for women with gynaecologic cancer.

Methods: The PRISMA guidelines were used to conduct this systematic review. We performed a comprehensive literature search in nine databases and qualitative studies published in English or Chinese from inception to January 2020. The included papers were appraised, using the Critical Appraisal Skills Program tool for qualitative research. An inductive thematic analysis method was adopted to synthesise major findings to construct core concepts and themes.

Results: Eight studies were included in this review, and four overarching themes emerged the following: the negative experience of disease, the need for supportive care to cope, adapting to a new life and post-traumatic growth.

Conclusions: This study shows that male partners had both negative and positive experiences in the caring process, and they could adjust themselves to some extent. Their perceived supportive care needs were often neglected.

Relevance To Clinical Practice: Male partners of women with gynaecologic cancer are an under-recognised group. The couple-oriented or family-oriented supportive care programmes should be implemented to meet the supportive care needs of male partners to enhance their health and well-being.
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http://dx.doi.org/10.1111/jocn.15501DOI Listing
December 2020

A oxygen vacancy-modulated homojunction structural CuBiO photocathodes for efficient solar water reduction.

Nanoscale 2020 Jul;12(28):15193-15200

College of Chemical Engineering, Northwest University for Nationalities, Lanzhou, Gansu 730030, P.R. China.

The photoelectrochemical (PEC) water reduction performance of CuBi2O4 (CBO)-based photocathodes is still far from their theoretical values due to low bulk and surface charge separation efficiencies. Herein, we propose a regrowth strategy to prepare a photocathode with CBO coating on Zn-doped CBO (CBO/Zn-CBO). Furthermore, NaBH4 treatment of CBO/Zn-CBO introduced oxygen vacancies (Ov) on CBO/Zn-CBO. It was found that Zn-doping not only increases the charge carrier concentration of CBO, but also leads to appropriate band alignment to form homojunctions. This homojunction can effectively promote the separation of electron-hole pairs, thus obtaining excellent photocurrent density (0.5 mA cm-2 at 0.3 V vs. RHE) and charge separation efficiency (1.5 times than CBO). The following surface treatment induced Ov on CBO/Zn-CBO, which significantly increased the active area of the surface catalytic reaction and further enhanced the photocurrent density (0.6 mA cm-2). In the absence of cocatalysts, the electron injection efficiency of Ov/CBO/Zn-CBO was 1.47 times improved than that of CBO. This work demonstrates a homojunction photocathode with Ov modulation, which provides a new view for future photoelectrochemical water splitting.
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http://dx.doi.org/10.1039/d0nr04473cDOI Listing
July 2020

Identification of -Phenyl-7-pyrrolo[2,3-]pyrimidin-4-amine Derivatives as Novel, Potent, and Selective NF-κB Inducing Kinase (NIK) Inhibitors for the Treatment of Psoriasis.

J Med Chem 2020 07 16;63(13):6748-6773. Epub 2020 Jun 16.

School of Sciences, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, P. R. China.

A series of -phenyl-7-pyrrolo[2,3-]pyrimidin-4-amine derivatives with NF-κB inducing kinase (NIK) inhibitory activity were obtained through structure-based drug design and synthetic chemistry. Among them, 4-(3-((7-pyrrolo[2,3-]pyrimidin-4-yl)amino)-4-morpholinophenyl)-2-(thiazol-2-yl)but-3-yn-2-ol () was identified as a highly potent NIK inhibitor, along with satisfactory selectivity. The pharmacokinetics of and its ability to inhibit interleukin 6 secretion in BEAS-2B cells were better than compound developed by Amgen. Oral administration of different doses of in an imiquimod-induced psoriasis mouse model showed effective alleviation of psoriasis, including invasive erythema, swelling, skin thickening, and scales. The underlying pathological mechanism involved attenuation of proinflammatory cytokine and chemokine gene expression, and the infiltration of macrophages after the treatment of . This work provides a foundation for the development of NIK inhibitors, highlighting the potential of developing NIK inhibitors as a new strategy for the treatment of psoriasis.
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http://dx.doi.org/10.1021/acs.jmedchem.0c00055DOI Listing
July 2020

Discovery of coumarin derivatives as potent and selective cyclin-dependent kinase 9 (CDK9) inhibitors with high antitumour activity.

Eur J Med Chem 2020 Aug 15;200:112424. Epub 2020 May 15.

School of Sciences, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, China; Laboratory of Molecular Design and Drug Discovery, School of Science, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China. Electronic address:

Specific inhibition of CDK9 is considered a promising strategy for developing effective anticancer therapeutics. However, most of the reported CDK9 inhibitors are still at an early stage of development and lack selectivity against other CDKs. Herein, we discovered coumarin derivative 30i as a potent CDK9 inhibitor with high selectivity (8300-fold over CDK7). Binding mode analysis illustrated that the substituent coumarin moiety is a critical group for CDK9 selectivity by occupying a flexible hinge/αD region, which is sterically hindered in other CDKs. Compound 30i showed excellent cellular antiproliferative activity, moderate pharmacokinetic property and low hERG inhibition. Moreover, 30i significantly induced tumour growth inhibition in a dose-dependent manner without causing an obvious loss of body weight in an MV4-11 xenograft mice model. Altogether, these results suggest that 30i may serve as a potential acute myeloid leukaemia (AML) therapeutics by selectively targeting CDK9.
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http://dx.doi.org/10.1016/j.ejmech.2020.112424DOI Listing
August 2020

Water-soluble low molecular weight organics in cloud water at Mt. Tai Mo Shan, Hong Kong.

Sci Total Environ 2019 Dec 26;697:134095. Epub 2019 Aug 26.

Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China; Institute of Surface-Earth System Science, Tianjin University, Tianjin 300072, China. Electronic address:

Cloud-water samples collected at the summit of Mt. Tai Mo Shan (Mt. TMS, 957 m, a.s.l.), Hong Kong in autumn 2016 and spring 2017 were measured for molecular compositions and stable carbon isotope ratios (δC) of dicarboxylic acids, oxoacids and α-dicarbonyls. Oxalic acid (C, 253-1680 μg L) was found as the most abundant diacid, followed by succinic acid (C, 24-656 μg L) in autumn and phthalic acid (Ph, 27-363 μg L) in spring. Higher concentrations of Ph (192 ± 197 μg L) and terephthalic acid (tPh, 31 ± 15 μg L) were observed in autumn than those in spring, illustrating the enhanced contribution from fossil fuel combustion and plastic wastes burning. Stronger correlations for the shorter chain diacids (C-C) with NO, nss-SO and nss-K in autumn (R ≥ 0.7) than spring suggested that these diacids were mainly produced via atmospheric photooxidation following anthropogenic emissions. The δC values of C (mean - 14.7‰), glyoxylic acid (ωC, -12.2‰), pyruvic acid (Pyr, -15.5‰), glyoxal (Gly, -13.5‰) were much higher than those in atmospheric aerosols from isoprene and other precursors, indicating that diacids, oxoacids and α-dicarbonyls in cloud at Mt. TMS were significantly influenced by photochemical formation during the long-range atmospheric transport.
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http://dx.doi.org/10.1016/j.scitotenv.2019.134095DOI Listing
December 2019

A congruence perspective on how human and social capital affect learning capability and innovation.

PLoS One 2020 13;15(4):e0231504. Epub 2020 Apr 13.

Central University of Finance and Economics, Beijing, China.

Human capital and social capital are vital for sustainable development of organization, but existing studies are inadequate to explore the synergistic effect of them on organizational behaviors or organizational outcomes. The research employed multiple-source questionnaire to collect data of more than 400 R&D firms from leaders and corresponding employees in Chinese context. Bootstrapping method and response surface were used to analyze the associations between all the parameters. The results showed that: (1) in the case of the in-congruence between human capital and social capital, learning capability of R&D firms is at highest level when human capital is at low level and social capital is at high level; (2) learning capability partially mediates the impact of human capital and social capital on innovation performance; (3) environmental dynamism and environmental competitiveness have a joint moderating effect on the relationship between learning capability and innovation performance.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231504PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153854PMC
July 2020

[Small dose of low molecular weight heparin improves the prognosis of elderly patients with severe pneumonia: a Meta-analysis of 1 173 patients].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Jan;32(1):26-32

Guizhou Medical University, Guiyang 550004, Guizhou, China.

Objective: To explore the effect of small dose of low molecular weight heparin on the prognosis of elderly patients with severe pneumonia using systematic evaluation method.

Methods: Databases including Wanfang data, VIP, CNKI, SinoMed, PubMed, Embase and Cochrane Library were searched for randomized controlled trial (RCT) studies about the comparison of conventional therapy and low molecular weight heparin on prognosis of elderly patients with severe pneumonia from the time of database establishment to August 2019. The patients in conventional treatment group were treated by improving ventilation, anti-infection, eliminating phlegm, relieving asthma and maintaining homeostasis while those in low molecular weight heparin group were subcutaneously injected with low molecular weight heparin of 4 000 U, once a day for 7 days. The patients' main outcomes included the oxygenation index (PaO/FiO) after 7 days of treatment, duration of mechanical ventilation, mortality in hospital, and secondary outcomes included acute physiology and chronic health evaluation II (APACHE II) score and coagulation function after 7 days of treatment, the length of intensive care unit (ICU) stay, and incidence of bleeding. Data extraction and quality evaluation were conducted. The Meta-analysis of included studies that met the quality standards was performed using RevMan 5.3 software. Funnel diagram analysis was used to analyze the parameters with no less than 10 studies enrolled.

Results: A total of 14 RCT studies were enrolled involving 1 173 elderly patients with severe pneumonia, among whom 590 received low molecular weight heparin while the other 583 received conventional therapy. All the included studies were well designed and of high quality. The results of Meta-analysis showed that compared with conventional therapy, small dose of low molecular weight heparin significantly elevated PaO/FiO after 7 days of treatment [mean difference (MD) = 19.25, 95% confidence interval (95%CI) was 16.88 to 21.61, P < 0.000 01], shortened the duration of mechanical ventilation (MD = -48.88, 95%CI was -67.42 to -30.33, P < 0.000 01), and decreased mortality in hospital [odds ratio (OR) = 0.40, 95%CI was 0.22 to 0.73, P = 0.003] and APACHE II score after 7 days of treatment (MD = -3.38, 95%CI was -3.94 to -2.83, P < 0.000 01), and shortened the length of ICU stay (MD = -4.51, 95%CI was -5.75 to -3.27, P < 0.000 01). There was no significant difference in the changes of coagulation parameters after 7 days of treatment or the incidence of bleeding between low molecular weight heparin group and conventional therapy group [7-day thrombin time (TT): MD = 0.57, 95%CI was -0.15 to 1.28, P = 0.12; 7-day prothrombin time (PT): MD = 0.32, 95%CI was -0.35 to 0.98, P = 0.35; 7-day fibrinogen (FIB): MD = -0.17, 95%CI was -0.45 to 0.10, P = 0.22; incidence of bleeding: OR = 0.86, 95%CI was 0.36 to 2.07, P = 0.74]. The funnel diagram showed that there was publication bias of included 10 studies about APACHE II score after 7 days of treatment.

Conclusions: Small dose of low molecular weight heparin can improve the prognosis of elderly patients with severe pneumonia and it has no obvious side-effect on coagulation function.
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http://dx.doi.org/10.3760/cma.j.cn121430-20191008-00005DOI Listing
January 2020

Distal tibial tuberosity high tibial osteotomy using an image enhancement technique for orthopedic scans in the treatment of medial compartment knee osteoarthritis.

Comput Methods Programs Biomed 2020 Jul 22;191:105349. Epub 2020 Jan 22.

Graduate school of Beijing University of Chinese Medicine, Beijing, China.

Objective: To observe the efficacy of distal tibial tuberosity high tibial osteotomy in treating medial compartment osteoarthritis of the knee. In the experiment, a medical image enhancement algorithm based on shear wave domain improved Gamma correction was implemented to process medical images in order to diagnose patients more effectively.

Methods: Sixty-five patients (13 males and 52 females) were included in the study who were treated with distal tibial tuberosity high tibial osteotomy, and the mean age of the patients was 63±6 years (range 49-73). All patients received X-Ray and MRI before surgery. We carefully studied and analyzed the findings, MRI findings and medical characteristics after using the shearlet domain to improve the Gamma correction method in order to enhance diagnostic accuracy. The improved image help us to judge the damage of the medial and lateral compartment more accurately. Weight bearing line (WBL) and femora-tibial Angle (FTA) were measured to evaluate the lower limb alignment before and 12 months after surgery. Visual analogue scale (VAS) scores, Hospital for Special Surgery (HSS) scores, the Westrn Ontario and Mcmaster Universities (WOMAC) scores were recorded to evaluate knee pain and function before surgery, 3 months and 6 months after surgery.

Results: The X-Ray and MRI scans of the patients were analyzed. After the shear wave shearlet domain is implemented, the image effect is significantly improved. 12 months after surgery, WBL was improved from 16.34±2.18% to 60.32±2.89% (p<0.05), FTA was improved from 171.17±1.87° to 179.83±2.14° (p<0.05). At 3 months and 12 months after surgery, VAS, HSS and WOMAC scores were significantly improved compared with baseline (p<0.05).

Conclusion: Distal tibial tuberosity high tibial osteotomy is able to correct varus deformity, relieve knee pain and the functional dysfunction, indicating which had a significant short-term efficacy. The shear wave shearlet domain incorporates the Gamma correction method to effectively preserve the image details, which to achieve a more accurate diagnosis, and help us to judge the damage of the medial and lateral compartment more accurately.
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http://dx.doi.org/10.1016/j.cmpb.2020.105349DOI Listing
July 2020

Activating a hematite nanorod photoanode via fluorine-doping and surface fluorination for enhanced oxygen evolution reaction.

Nanoscale 2020 Feb 23;12(5):3259-3266. Epub 2020 Jan 23.

State Key Laboratory of Applied Organic Chemistry (SKLAOC), The Key Laboratory of Catalytic Engineering of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, P. R. China.

Poor charge separation and sluggish oxygen evolution reaction (OER) kinetics are two typical factors that hinder the photoelectrochemical (PEC) applications of hematite. Dual modification via heteroatom doping and surface treatment is an attractive strategy to overcome the above problems. Herein, for the first time, a hematite nanorod photoanode was ameliorated via the fluorine treatment (F-treatment) of both bulk and surface, enabling simultaneous charge separation from the interior to the interface. Accordingly, the novel photoanode (FeF/F-FeO) exhibited an outstanding PEC water oxidation activity, with a 3-fold improved photocurrent density than that obtained using unmodified α-FeO. More specifically, fluorine doping (F-doping) in the hematite bulk remarkably increased the concentration of charge carriers and endowed it with favorable electrical conductivity for rapid charge transfer. Further surface F-treatment on F-doped α-FeO (F-FeO) enriched the F-Fe bonds on the surface, which significantly boosted the OER kinetics and thereby inhibited the detrimental charge recombination. As a consequence, the efficiencies of bulk electron-hole pair separation and surface hole injection increased by 2.8 and 1.7 times, respectively. This study points to fluorine modulation as an attractive avenue to advance the PEC performance of metal oxide-based photoelectrode materials.
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http://dx.doi.org/10.1039/c9nr09502kDOI Listing
February 2020

[Xuebijing improves clinical prognosis and reduces mortality in patients with acute paraquat poisoning: a Meta-analysis included 1 429 patients].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2019 Nov;31(11):1416-1422

Guizhou Medical University, Guiyang 550004, Guizhou, China.

Objective: To explore the therapeutic effect of Xuebijing on patients with acute paraquat poisoning (APP) by using systematic evaluation method.

Methods: PubMed, Cochrane Library, Embase, Wanfang database, China National Knowledge Infrastructure (CNKI), VIP database (VIP) and China Biology Medicine (CBM) were searched using the computers to find the literatures published about the Xuebijing injection for the treatment of APP. Randomized controlled trials (RCT) were retrieved from the establishment of the database to August 2019. Patients in experimental group were treated with Xuebijing injection combined with conventional treatment, while the patients in control group were only given conventional treatment. The patients' outcome included the 14-day mortality, arterial oxygen saturation (SaO) and incidence of pulmonary fibrosis. In addition, the 6-month survival rate, alanine aminotransferase (ALT), serum creatinine (SCr), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA) and superoxide dismutase (SOD) between the two groups were compared. The literature data were extracted by two researchers independently, and the quality of the literatures was evaluated according to the Cochrane 5.1 handbook. The Meta-analysis was performed by using RevMan 5.3 software. The results stability of Meta-analysis was tested by sensitivity analysis. The publication bias was analyzed through drawing of funnel diagram.

Results: Twenty-seven RCT studies in total were enrolled, of which 26 were in Chinese and 1 was in English. A total of 1 429 patients were enrolled, among whom 726 were in experimental group and another 703 were in control group. Meta-analysis showed that compared with the control group, the 14-day mortality [relative risk (RR) = 0.62, 95% confidence interval (95%CI) was 0.54 to 0.72, P < 0.000 01] and incidence of pulmonary fibrosis (RR = 0.67, 95%CI was 0.53 to 0.85, P = 0.000 9) of patients in the experimental group were significantly lowered, while SaO at 7 days and 14 days were significantly increased [7 days: mean difference (MD) = 16.86, 95%CI was 9.89 to 23.83, P < 0.000 01; 14 days: MD = 16.51, 95%CI was 10.22 to 22.80, P < 0.000 01]. Compared with the control group, the survival rate within 6 months (RR = 1.55, 95%CI was 1.41 to 1.71, P < 0.000 01) and SOD (MD = 13.88, 95%CI was 7.43 to 20.33, P < 0.000 1) of patients in the experimental group were significantly increased, ALT at 14 days (MD = -78.35, 95%CI was -127.35 to -29.34, P = 0.000 5), SCr at 7 days and 14 days (7 days: MD = -135.13, 95%CI was -219.09 to -51.17, P = 0.002; 14 days: MD = -206.05, 95%CI = -290.13 to -121.96, P < 0.000 01), CRP (MD = -11.55, 95%CI was -17.77 to -5.33, P = 0.000 3), TNF-α (MD = -9.27, 95%CI was -15.48 to -3.96, P = 0.000 9) and MDA (MD = -1.27, 95%CI was -1.57 to -0.96, P < 0.000 01) were significantly lowered. The overall effect value of the parameters with high heterogeneity was not significantly changed after further Meta-analysis excluding any one of the studies, suggesting that the result was relatively stable. Funnel chart analysis was used to analyze the parameters from more than 10 articles enrolled, and it showed that there was publication bias.

Conclusions: Xuebijing injection can reduce the mortality of patients with APP, which may because that it can improve liver and kidney function, reduce inflammation and oxidative stress damage, inhibit pulmonary fibrosis and increase oxygenation level.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2019.11.020DOI Listing
November 2019

Synthesis of Metal Phosphide Nanoparticles Supported on Porous N-Doped Carbon Derived from Spirulina for Universal-pH Hydrogen Evolution.

ChemSusChem 2020 Jan 12;13(2):351-359. Epub 2019 Dec 12.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, P. R. China.

Transition metal phosphides (TMPs) are regarded as highly active electrocatalysts for the hydrogen evolution reaction (HER). However, traditional synthetic routes usually use expensive and dangerous precursors as P donors. The development of a low-cost and ecofriendly method for the synthesis of TMPs is significant for sustainable energy development. Herein, cobalt phosphides anchored on or embedded in a spirulina-derived porous N-doped carbon matrix (Co P/NC) was fabricated by two-step hydrothermal treatment and carbonization method, which utilized the intrinsic C, N, and P of biomass cleverly as the sources of C, N, and P, respectively. As a result of the high surface area and porosity that enhance the mass-transfer dynamics, Co P/NC shows good electrocatalytic activity at all pH values in the HER. This work not only provides a facile and effective method for the fabrication of TMP nanoparticles loaded onto carbon materials but also opens a new strategy for the utilization of the intrinsic ingredients of biomass for the preparation of other functional electrocatalysts.
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http://dx.doi.org/10.1002/cssc.201902920DOI Listing
January 2020

Herbivore exposure alters ion fluxes and improves salt tolerance in a desert shrub.

Plant Cell Environ 2020 02 6;43(2):400-419. Epub 2019 Dec 6.

College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

Plants have evolved complex mechanisms that allow them to withstand multiple environmental stresses, including biotic and abiotic stresses. Here, we investigated the interaction between herbivore exposure and salt stress of Ammopiptanthus nanus, a desert shrub. We found that jasmonic acid (JA) was involved in plant responses to both herbivore attack and salt stress, leading to an increased NaCl stress tolerance for herbivore-pretreated plants and increase in K /Na ratio in roots. Further evidence revealed the mechanism by which herbivore improved plant NaCl tolerance. Herbivore pretreatment reduced K efflux and increased Na efflux in plants subjected to long-term, short-term, or transient NaCl stress. Moreover, herbivore pretreatment promoted H efflux by increasing plasma membrane H -adenosine triphosphate (ATP)ase activity. This H efflux creates a transmembrane proton motive force that drives the Na /H antiporter to expel excess Na into the external medium. In addition, high cytosolic Ca was observed in the roots of herbivore-treated plants exposed to NaCl, and this effect may be regulated by H -ATPase. Taken together, herbivore exposure enhances A. nanus tolerance to salt stress by activating the JA-signalling pathway, increasing plasma membrane H -ATPase activity, promoting cytosolic Ca accumulation, and then restricting K leakage and reducing Na accumulation in the cytosol.
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http://dx.doi.org/10.1111/pce.13662DOI Listing
February 2020

Medical imaging and diagnosis of subpatellar vertebrae based on improved Laplacian image enhancement algorithm.

Comput Methods Programs Biomed 2020 Apr 16;187:105082. Epub 2019 Sep 16.

Minimal Invasive Joint Department, The Third Affiliated Hospital of Beijing University of Chinese Medicine, No. 51 Anwai Xiaoguan Street, Chaoyang District, Beijing, 100029, China.

Objective: Nowadays, arthroscopy is widely applied to the treatment of joint diseases. The experimental trials were designed to determine whether the infrapatellar plica was symptomatic or not, and to appraise the medical effects of these patients who underwent arthroscopic treatment. An improved Laplacian image enhancement algorithm is added to the experiment. The medical image of the Subpatellar vertebral body under arthroscope is processed by the algorithm. The processed image is compared with the original image, and the advantages and disadvantages of the improved Laplacian image enhancement algorithm are analyzed.

Methods: Retrospective Medical trial design was executed in our study. In addition, X-ray film and magnetic resonance imaging (MRI) were included in the study. Visual Analogue Scale (VAS) and Lysholm Score were carried out. Arthroscopy results, MRI findings, and Medical features were researched and analyzed carefully. Then we use the improved Laplacian image enhancement algorithm to process the image, which makes the image more convenient for analysis and improves the diagnostic accuracy.

Results: Some of the experimental protomedical images are not clear enough, and the details and textures are difficult to judge, which hinders the diagnosis. After the improved Laplacian algorithm processing, the image effect has been significantly improved. From the image we get the result, although the wound healed after surgery, some patients have existence of transient swelling in recovery process but no effusion. The pain of all patients knee was sharply relieved and the function was improved. All patients' conditions were most satisfactory.

Conclusion: The findings in this study demonstrate a significant reduction in knee pain and improvement in function by releasing and removal of the symptomatic infrapatellar plica under arthroscopic surgery. The image processed by the improved Laplacian image enhancement algorithm can effectively retain the image details, which is conducive to diagnosis and improve the diagnostic accuracy.
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http://dx.doi.org/10.1016/j.cmpb.2019.105082DOI Listing
April 2020

Conformally Coupling CoAl-Layered Double Hydroxides on Fluorine-Doped Hematite: Surface and Bulk Co-Modification for Enhanced Photoelectrochemical Water Oxidation.

ACS Appl Mater Interfaces 2019 Aug 12;11(33):29799-29806. Epub 2019 Aug 12.

Earth-abundant hematite is an attractive photoanode for photoelectrochemical water splitting, whereas the intrinsic properties of inferior charge transfer and slow water oxidation kinetics still hinder its application. In response, an integrated photoanode has been constructed with hematite nanorod arrays modified by fluorine anion doping and further decorated with amorphous CoAl-layered double hydroxides (CoAl-LDH). This novel CoAl-LDH/F-FeO photoanode exhibited an excellent photocurrent density of 2.46 mA cm at 1.23 V versus reversible hydrogen electrode (), five times enhanced than that of pristine α-FeO. Systematic investigations reveal that fluorine anion serving as a donor dopant dramatically enhances the density of charge carrier and reduces the resistance of hematite for rapid charge transfer. Furthermore, the cocatalyst of CoAl-LDH could effectively passivate the surface defects of F-FeO and facilitate the water oxidation kinetics through an alternative pathway of holes trapped by Co species. As a consequence, the charge separation efficiencies of the bulk and surface were improved to 32.6 and 81.8%, respectively, compared with those of α-FeO (9.7 and 31.7%). Our results demonstrate that the dual modification of the bulk and surface is an attractive maneuver to ameliorate the water oxidation activity of hematite.
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http://dx.doi.org/10.1021/acsami.9b07417DOI Listing
August 2019

An intervention based on protective factors to improve resilience for breast cancer patients: Study protocol for a randomized controlled trial.

J Adv Nurs 2019 Nov 14;75(11):3088-3096. Epub 2019 Aug 14.

School of Nursing, Anhui Medical University, Hefei, China.

Aim: To present a study protocol for evaluating the feasibility and effectiveness of an individual face-to-face intervention based on protective factors to improve resilience among breast cancer patients.

Background: Research involving the effectiveness of universal interventions to improve resilience for breast cancer patients has seldom been reported and there is an urgent need to find a more acceptable, cost-effective method of providing emotional support. Through health education and psychological interventions, increasing protective factors could promote recovery to the original condition and improve resilience.

Design: A randomized controlled trial of an individual face-to-face intervention.

Methods: A total of 160 adults diagnosed with confirmed breast cancer will be recruited. The patients will be randomly assigned to the control group (N = 80) or the intervention group (N = 80). An intervention focusing on protective factors will be implemented in the intervention group. A survey of the patients from the two groups will be conducted at baseline, 1, 3, 6, and 12 months. The primary outcome is resilience, measured by the 14-item Resilience Scale. Secondary outcomes include self-efficacy, optimism, perceived social support, and mastery. The Chinese versions of the General Self-Efficacy Scale, revised Life Orientation Test, Multidimensional Scale of Perceived Social Support, and Self-Mastery Scale will be used to measure the four protective factors.

Impact: One-to-one and face-to-face interventions have many potential advantages for inpatients, including convenience, accessibility and individuality. Once its effectiveness is confirmed, the intervention will be implemented broadly and make support available for a large number of patients.
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http://dx.doi.org/10.1111/jan.14108DOI Listing
November 2019

Recent progress in the synthesis and applications of 2D metal nanosheets.

Nanotechnology 2019 May 11;30(22):222001. Epub 2019 Feb 11.

The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, People's Republic of China.

The design and controlled synthesis of two-dimensional (2D) nanomaterials have been widely studied because the properties and functions of nanomaterials are highly dependent on their sizes, shapes, and dimensionalities. For instance, 2D metal nanosheets (2DMNSs) have attracted a significant amount of attention owing to their interesting properties, which are absent in corresponding bulk counterparts, and they have been confirmed to have potential applications in electrocatalysis, optics, and biomedicine. However, because of the close-packed structures of metals, the large-scale fabrication of 2DMNSs is challenging. In this review, we have outlined the research progress in the field of 2DMNSs, including the typical synthesis approaches and newly developed methods, as well as promising applications of the materials reported in recent years. Moreover, some preliminary and promising strategies to further improve the properties of 2DMNSs and some insights for the development of the field have been included.
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http://dx.doi.org/10.1088/1361-6528/ab0642DOI Listing
May 2019

Factors Associated with Low Admission Platelet Count in Adults with Acute Aortic Dissection.

Ann Thorac Cardiovasc Surg 2019 Jun 18;25(3):142-148. Epub 2018 Dec 18.

Department of Anaesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Purpose: Platelets are crucial components of the coagulation processes, and low admission platelet count (PLC) is associated with adverse clinical outcomes in patients with Stanford type A acute aortic dissection (AAD).

Methods: A total of 130 consecutive patients undergoing Stanford type A AAD surgery in Beijing Anzhen Hospital were enrolled between January 2013 and July 2014. Preoperative clinical and laboratory data from patients were collected. Multiple regression analyses were used to determine the independent factors of low admission platelets.

Results: Adjusted multiple regression analysis showed that age (β: -1.069, 95% confidence interval [CI]: -2.109, -0.029), sex (β: -29.973, 95% CI: -56.512, -3.433), tissue factor pathway inhibitor (TFPI; β: 0.197, 95% CI: 0.039, 0.354), fibrinogen degradation product (FDP) (β: -0.476, 95% CI: -0.879, -0.074), and attack time (β: 11.125, 95% CI: 7.963, 14.287) were significantly associated with admission PLC. Admission PLC increased with attack time up to the 3 days (β: 16.2, 95% CI: 12.1, 20.2).

Conclusions: We found that increasing age, male patients, patients with lower serum levels of TFPI and higher serum levels of FDP, and patients with a shorter attack time were significantly associated with lower PLC at admission. Moreover, the turning point of attack time is 3 days after the onset of dissection.
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http://dx.doi.org/10.5761/atcs.oa.18-00187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587131PMC
June 2019

Characterization and source identification of PM-bound polycyclic aromatic hydrocarbons (PAHs) in different seasons from Shanghai, China.

Sci Total Environ 2018 Dec 11;644:725-735. Epub 2018 Jul 11.

The Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, Shanghai 200241, China.

PM samples in four representative periods were collected from a highly industrialized district in Shanghai, China. The concentrations of PM and PM-bound PAHs were analyzed. Positive matrix factorization (PMF) model was used to identify the potential sources. Relationship between PAHs distribution and meteorological parameters was assessed meanwhile. The incremental lifetime cancer risks (ILCRs) model was applied to quantitatively evaluate the exposure risk of PAHs. Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLT) model was used to track the potential pollution area of PM along with a Potential Source Contribution Function (PSCF) and Concentration Weighted Trajectory (CWT) methods. The results showed concentrations of PM and PAHs ranged from 14.83 to 185.58 μg/m, 2.58 to 123.62 ng/m, respectively. The source apportionment model indicated that traffic emissions were the most important sources in each sampling season, which accounted for 38.44%, 34.48%, 39.04% and 45.03%, respectively. Spearman correlation coefficient showed that PAHs had negative correlation with ambient temperature and relative humidity in some periods, while had no significant correlation with atmospheric pressure and visibility. The average estimated lifetime cancer risk for total PAHs reached 4.7 × 10, 4.5 × 10 and 4.1 × 10, 4.0 × 10 to exposed children and adults in winter and autumn, respectively, meaning that PM-bound PAHs had high potential risk. HYSPLIT model suggested that monsoon greatly influenced the air quality in both winter and autumn.
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http://dx.doi.org/10.1016/j.scitotenv.2018.07.049DOI Listing
December 2018

NiO Nanoparticles Anchored on Phosphorus-Doped α-Fe O Nanoarrays: An Efficient Hole Extraction p-n Heterojunction Photoanode for Water Oxidation.

ChemSusChem 2018 Jul 13;11(13):2156-2164. Epub 2018 Jun 13.

State Key Laboratory of Applied Organic Chemistry (SKLAOC), The Key Laboratory of Catalytic Engineering of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu, 730000, PR China.

The photoelectrochemical (PEC) water-splitting efficiency of a hematite-based photoanode is still far from the theoretical value due to its poor surface reaction kinetics and high density of surface trapping states. To solve these drawbacks, a photoanode consisting of NiO nanoparticles anchored on a gradient phosphorus-doped α-Fe O nanorod (NR) array (NiO/P-α-Fe O ) was fabricated to achieve optimal light absorption and charge separation, as well as rapid surface reaction kinetics. Specifically, a photoanode with the NR array structure allowed a high mass-transport rate to be achieved, while phosphorus doping effectively decreased the number of surface trapping sites and improved the electrical conductivity of α-Fe O . Furthermore, the p-n junction that forms between NiO and P-α-Fe O can further improve the PEC performance due to efficient hole extraction and the water oxidization catalytic activity of NiO. Consequently, the NiO/P-α-Fe O NR photoanode produced a high photocurrent density of 2.08 mA cm at 1.23 V versus a reversible hydrogen electrode and a 110 mV cathodic shift of the onset potential. This rational design of structure offers a new perspective in exploring high-performance PEC photoanodes.
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http://dx.doi.org/10.1002/cssc.201800571DOI Listing
July 2018