Publications by authors named "Shuwei Liu"

151 Publications

Enhancing Tumor Therapy of Fe(III)-Shikonin Supramolecular Nanomedicine via Triple Ferroptosis Amplification.

ACS Appl Mater Interfaces 2022 Aug 9. Epub 2022 Aug 9.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Ferroptosis has been considered as a promising pathway to overcome apoptosis-induced tumor chemoresistance. However, the antitumor efficacy of ferroptosis-inducing agents is still limited because of the complexity and diversity of tumor microenvironments. Herein, we demonstrate a triple ferroptosis amplification strategy for tumor therapy by associating iron-based nanocarriers, ferroptosis molecular drugs, and HO-producing enzymes. Fe(III)-Shikonin (FeShik) metal-polyphenol-coordinated networks are employed to load a ferroptosis inducer of sorafenib (SRF) inside and glucose oxidase (GOx) outside, thus producing [email protected] supramolecular nanomedicines (SNs). After delivering into glutathione (GSH)-overexpressed tumor cells, FeShik will disassemble and release Fe to induce cell death via ferroptosis. At the same time, GOx executes its catalytic activity to produce an acid environment and plenty of HO for stimulating OH generation via the Fenton reaction. Moreover, SRF will suppress the biosynthesis of GSH by inhibiting system Xc, further deactivating the enzymatic activity of glutathione peroxidase 4 (GPX4). Up-regulation of the oxidative stress level and down-regulation of GPX4 expression can dramatically accelerate the accumulation of lethal lipid peroxides, leading to ferroptosis amplification of tumor cells. The current strategy that utilizes ferroptosis-inducing agents as both nanocarriers and cargoes provides a pathway to enhance the efficacy of ferroptosis-based tumor therapy.
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http://dx.doi.org/10.1021/acsami.2c11130DOI Listing
August 2022

TaSRO1 plays a dual role in suppressing TaSIP1 to fine tune mitochondrial retrograde signalling and enhance salinity stress tolerance.

New Phytol 2022 Jun 24. Epub 2022 Jun 24.

The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.

Initially discovered in yeast, mitochondrial retrograde signalling has long been recognised as an essential in the perception of stress by eukaryotes. However, how to maintain the optimal amplitude and duration of its activation under natural stress conditions remains elusive in plants. Here, we show that TaSRO1, a major contributor to the agronomic performance of bread wheat plants exposed to salinity stress, interacted with a transmembrane domain-containing NAC transcription factor TaSIP1, which could translocate from the endoplasmic reticulum (ER) into the nucleus and activate some mitochondrial dysfunction stimulon (MDS) genes. Overexpression of TaSIP1 and TaSIP1-∆C (a form lacking the transmembrane domain) in wheat both compromised the plants' tolerance of salinity stress, highlighting the importance of precise regulation of this signal cascade during salinity stress. The interaction of TaSRO1/TaSIP1, in the cytoplasm, arrested more TaSIP1 on the membrane of ER, and in the nucleus, attenuated the trans-activation activity of TaSIP1, therefore reducing the TaSIP1-mediated activation of MDS genes. Moreover, the overexpression of TaSRO1 rescued the inferior phenotype induced by TaSIP1 overexpression. Our study provides an orchestrating mechanism executed by the TaSRO1-TaSIP1 module that balances the growth and stress response via fine tuning the level of mitochondria retrograde signalling.
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http://dx.doi.org/10.1111/nph.18340DOI Listing
June 2022

Erratum: Tumor Theranostics of Transition Metal Ions Loaded Polyaminopyrrole Nanoparticles: Erratum.

Nanotheranostics 2022 18;6(3):322-324. Epub 2022 Feb 18.

State Key Supramolecular Structure and Materials Laboratory, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

[This corrects the article DOI: 10.7150/ntno.25119.].
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http://dx.doi.org/10.7150/ntno.67620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194588PMC
February 2022

Functional analysis of long noncoding RNAs involved in alkaline stress responses in wheat.

J Exp Bot 2022 May 20. Epub 2022 May 20.

Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, China.

Saline-alkali soil is a key environmental problem affecting crop productivity. One of the most effective approaches to combat it is to breed stress-tolerant plants through genetic engineering. Shanrong No. 4 (SR4) is an alkaline-tolerant bread wheat cultivar derived from asymmetric somatic hybridization between the common wheat cultivar Jinan 177 (JN177) and tall wheatgrass. We aimed to explore the structure and function of alkalinity stress-responsive long noncoding RNAs (lncRNAs) in wheat. In this study, lncRNA sequencing was employed to identify stress tolerance-associated lncRNAs and their corresponding targets. Approximately 19,000 novel lncRNA sequences were detected in SR4 and JN177. Upon exposure to alkaline stress, SR4 differentially expressed 5691 lncRNAs, whereas JN177 differentially expressed 5932 lncRNAs. We selected five differentially expressed lncRNAs (L0760, L6247, L0208, L2098 and L3065) and generated transiently knocked down strains of wheat seedlings using the virus-induced gene silencing method. L0760 and L2098 knockdown caused the plants to exhibit sensitivity to alkaline stress, whereas L6247, L0208 and L3065 knockdown increased the ability of plants to tolerate alkaline stress. lncRNA-miRNA-target mRNA network and alkali-response-related lncRNA-target mRNA association networks were constructed to analyze the function of lncRNAs. Collectively, our results demonstrate that lncRNAs may have different roles under alkaline stress conditions.
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http://dx.doi.org/10.1093/jxb/erac211DOI Listing
May 2022

Global methane and nitrous oxide emissions from inland waters and estuaries.

Glob Chang Biol 2022 Aug 27;28(15):4713-4725. Epub 2022 May 27.

Key Laboratory of Low-carbon and Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Inland waters (rivers, reservoirs, lakes, ponds, streams) and estuaries are significant emitters of methane (CH ) and nitrous oxide (N O) to the atmosphere, while global estimates of these emissions have been hampered due to the lack of a worldwide comprehensive data set of CH and N O flux components. Here, we synthesize 2997 in-situ flux or concentration measurements of CH and N O from 277 peer-reviewed publications to estimate global CH and N O emissions from inland waters and estuaries. Inland waters including rivers, reservoirs, lakes, and streams together release 95.18 Tg CH  year (ebullition plus diffusion) and 1.48 Tg N O year (diffusion) to the atmosphere, yielding an overall CO -equivalent emission total of 3.06 Pg CO  year . The estimate of CH and N O emissions represents roughly 60% of CO emissions (5.13 Pg CO  year ) from these four inland aquatic systems, among which lakes act as the largest emitter for both CH and N O. Ebullition showed as a dominant flux component of CH , contributing up to 62%-84% of total CH fluxes across all inland waters. Chamber-derived CH emission rates are significantly greater than those determined by diffusion model-based methods for commonly capturing of both diffusive and ebullitive fluxes. Water dissolved oxygen (DO) showed as a dominant factor among all variables to influence both CH (diffusive and ebullitive) and N O fluxes from inland waters. Our study reveals a major oversight in regional and global CH budgets from inland waters, caused by neglecting the dominant role of ebullition pathways in those emissions. The estimated indirect N O EF values suggest that a downward refinement is required in current IPCC default EF values for inland waters and estuaries. Our findings further indicate that a comprehensive understanding of the magnitude and patterns of CH and N O emissions from inland waters and estuaries is essential in defining the way of how these aquatic systems will shape our climate.
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http://dx.doi.org/10.1111/gcb.16233DOI Listing
August 2022

Copper Ion and Ruthenium Complex Codoped Polydopamine Nanoparticles for Magnetic Resonance/Photoacoustic Tomography Imaging-Guided Photodynamic/Photothermal Dual-Mode Therapy.

ACS Appl Bio Mater 2022 05 4;5(5):2365-2376. Epub 2022 May 4.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Phototherapy, such as photodynamic therapy (PDT) and photothermal therapy (PTT), refers to the therapeutic strategy using a visible or near-infrared (NIR) laser to generate free radicals or heat for noninvasive and localized tumor treatment. However, limited by the low photoconversion efficiency of therapeutic agents, a single treatment method can hardly lead to complete tumor ablation, even when enhancing the power density of the laser and/or prolonging the irradiation duration. In this work, copper ion and ruthenium complex codoped polydopamine nanoparticles (Cu(II)/LRu/PDA NPs) are designed for PDT/PTT dual-mode therapy. The doped LRu in the NPs can generate reactive oxygen species under visible laser irradiation and enable PDT. Because of the strong absorption in the NIR region, PDA can not only generate heat for PTT under irradiation but also be used for photoacoustic tomography (PAT) imaging. Meanwhile, the doping of Cu(II) in the NPs through the coordination with PDA facilitates T-weighted magnetic resonance imaging (MRI). Thus, MR/PAT imaging-guided PDT/PTT dual-mode therapy is achieved. The in vivo experiments indicate that the Cu(II)/LRu/PDA NPs can accumulate in HeLa tumors with a retention rate up to 8.34%ID/g. MR/PAT imaging can clearly identify the location and boundary of the tumors, permitting precise guidance for phototherapy. Under the combined effect of PDT and PTT, a complete ablation of HeLa tumors is achieved. The current work provides an alternative nanoplatform for performing PDT/PTT dual-mode therapy, which can be further guided by MR/PAT imaging.
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http://dx.doi.org/10.1021/acsabm.2c00212DOI Listing
May 2022

Morphological and hemispheric and sex differences of the anterior ascending ramus and the horizontal ascending ramus of the lateral sulcus.

Brain Struct Funct 2022 Jul 20;227(6):1949-1961. Epub 2022 Apr 20.

Department of Anatomy and Neurobiology, Research Center for Sectional and Imaging Anatomy, Shandong Provincial Key Laboratory of Mental Disorder, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.

Broca's area is composed of the pars opercularis (PO) and the pars triangularis (PTR) of the inferior frontal gyrus; the anterior ascending ramus of the lateral sulcus (aals) separates the PO from the PTR, and the horizontal ascending ramus of the lateral sulcus (hals) separates the PTR from the pars orbitalis. The morphometry of these two sulci maybe has potential effects on the various functions of Broca's area. Exploring the morphological variations, hemispheric differences and sex differences of these two sulci contributed to a better localization of Broca's area. BrainVISA was used to reconstruct and parameterize these two sulci based on data from 3D MR images of 90 healthy right-handed subjects. The 3D anatomic morphologies of these two sulci were investigated using 4 sulcal parameters: average depth (AD), average width (AW), outer length (OL) and inner length (IL). The aals and hals could be identified in 98.89% and 98.33%, respectively, of the hemispheres evaluated. The morphological patterns of these two sulci were categorized into four typical types. There were no statistically significant interhemispheric or sex differences in the frequency of the morphological patterns. There was statistically significant interhemispheric difference in the IL of the aals. Significant sex differences were found in the AD and the IL of the aals and OL of the hals. Our results not only provide a structural basis for functional studies related to Broca's area but also are helpful in determining the precise position of Broca's area in neurosurgery.
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http://dx.doi.org/10.1007/s00429-022-02482-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232435PMC
July 2022

Altered cortical microstructure in preterm infants at term-equivalent age relative to term-born neonates.

Cereb Cortex 2022 Mar 8. Epub 2022 Mar 8.

Department of Anatomy and Neurobiology, Research Center for Sectional and Imaging Anatomy, Shandong Key Laboratory of Mental Disorders, Shandong Key Laboratory of Digital Human and Clinical Anatomy, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Preterm (PT) birth is a potential factor for abnormal brain development. Although various alterations of cortical structure and functional connectivity in preterm infants have been reported, the underlying microstructural foundation is still undetected thoroughly in PT infants relative to full-term (FT) neonates. To detect the very early cortical microstructural alteration noninvasively with advanced neurite orientation dispersion and density imaging (NODDI) on a whole-brain basis, we used multi-shell diffusion MRI of healthy newborns selected from the Developing Human Connectome Project. 73 PT infants and 69 FT neonates scanned at term-equivalent age were included in this study. By extracting the core voxels of gray matter (GM) using GM-based spatial statistics (GBSS), we found that comparing to FT neonates, infants born preterm showed extensive lower neurite density in both primary and higher-order association cortices (FWE corrected, P < 0.025). Higher orientation dispersion was only found in very preterm subgroup in the orbitofrontal cortex, fronto-insular cortex, entorhinal cortex, a portion of posterior cingular gyrus, and medial parieto-occipital cortex. This study provided new insights into exploring structural MR for functional and behavioral variations in preterm population, and these findings may have marked clinical importance, particularly in the guidance of ameliorating the development of premature brain.
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http://dx.doi.org/10.1093/cercor/bhac091DOI Listing
March 2022

Morphological Development of the Human Fetal Striatum During the Second Trimester.

Cereb Cortex 2022 Jan 25. Epub 2022 Jan 25.

The morphological development of the fetal striatum during the second trimester has remained poorly described. We manually segmented the striatum using 7.0-T MR images of the fetal specimens ranging from 14 to 22 gestational weeks. The global development of the striatum was evaluated by volume measurement. The absolute volume (Vabs) of the caudate nucleus (CN) increased linearly with gestational age, while the relative volume (Vrel) showed a quadratic growth. Both Vabs and Vrel of putamen increased linearly. Through shape analysis, the changes of local structure in developing striatum were specifically demonstrated. Except for the CN tail, the lateral and medial parts of the CN grew faster than the middle regions, with a clear rostral-caudal growth gradient as well as a distinct "outside-in" growth gradient. For putamen, the dorsal and ventral regions grew obviously faster than the other regions, with a dorsal-ventral bidirectional developmental pattern. The right CN was larger than the left, whereas there was no significant hemispheric asymmetry in the putamen. By establishing the developmental trajectories, spatial heterochrony, and hemispheric dimorphism of human fetal striatum, these data bring new insight into the fetal striatum development and provide detailed anatomical references for future striatal studies.
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http://dx.doi.org/10.1093/cercor/bhab532DOI Listing
January 2022

Decreased DNA Methylation of RGMA is Associated with Intracranial Hypertension After Severe Traumatic Brain Injury: An Exploratory Epigenome-Wide Association Study.

Neurocrit Care 2022 08 13;37(1):26-37. Epub 2022 Jan 13.

Department of Neurology, Neurobiology and Neurosurgery, Barrow Neurological Institute and St. Joseph's Hospital and Medical Center, 240 West Thomas Road, Phoenix, AZ, 85013, USA.

Background: Cerebral edema and intracranial hypertension are major contributors to unfavorable prognosis in traumatic brain injury (TBI). Local epigenetic changes, particularly in DNA methylation, may influence gene expression and thus host response/secondary injury after TBI. It remains unknown whether DNA methylation in the central nervous system is associated with cerebral edema severity or intracranial hypertension post TBI. We sought to identify epigenome-wide DNA methylation patterns associated with these forms of secondary injury after TBI.

Methods: We obtained genome-wide DNA methylation profiles of DNA extracted from ventricular cerebrospinal fluid samples at three different postinjury time points from a prospective cohort of patients with severe TBI (n = 89 patients, 254 samples). Cerebral edema and intracranial pressure (ICP) measures were clustered to generate composite end points of cerebral edema and ICP severity. We performed an unbiased epigenome-wide association study (EWAS) to test associations between DNA methylation at 419,895 cytosine-phosphate-guanine (CpG) sites and cerebral edema/ICP severity categories. Given inflated p values, we conducted permutation tests for top CpG sites to filter out potential false discoveries.

Results: Our data-driven hierarchical clustering across six cerebral edema and ICP measures identified two groups differing significantly in ICP based on the EWAS-identified CpG site cg22111818 in RGMA (Repulsive guidance molecule A, permutation p = 4.20 × 10). At 3-4 days post TBI, patients with severe intracranial hypertension had significantly lower levels of methylation at cg22111818.

Conclusions: We report a novel potential relationship between intracranial hypertension after TBI and an acute, nonsustained reduction in DNA methylation at cg22111818 in the RGMA gene. To our knowledge, this is the largest EWAS in severe TBI. Our findings are further strengthened by previous findings that RGMA modulates axonal repair in other central nervous system disorders, but a role in intracranial hypertension or TBI has not been previously identified. Additional work is warranted to validate and extend these findings, including assessment of its possible role in risk stratification, identification of novel druggable targets, and ultimately our ability to personalize therapy in TBI.
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http://dx.doi.org/10.1007/s12028-021-01424-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287123PMC
August 2022

Fe(III)-Doped Polyaminopyrrole Nanoparticle for Imaging-Guided Photothermal Therapy of Bladder Cancer.

ACS Biomater Sci Eng 2022 02 11;8(2):502-511. Epub 2022 Jan 11.

Optical Functional Theranostics Joint Laboratory of Medicine and Chemistry, The First Hospital of Jilin University, Changchun 130021, P. R. China.

Clinically, the surgical treatment of bladder cancer often faces the problem of tumor recurrence, and the surgical treatment combined with postoperative chemotherapy to inhibit tumor recurrence also faces high toxicity and side effects. Therefore, the need for innovative bladder cancer treatments is urgent. For the past few years, with the development of nano science and technology, imaging-guided therapy using nanomaterials with both imaging and therapy functions has shown great advantages and can not only identify the locations of the tumors but also exhibit biodistributions of nanomaterials in the tumors, significantly improving the accuracy and efficacy of treatment. In this work, we synthesized Fe(III)-doped polyaminopyrrole nanoparticles (FePPy-NH NPs). With low cytotoxicity and a blood circulation half-life of 7.59 h, high levels of FePPy-NH NPs accumulated in bladder tumors, with an accumulation rate of up to 5.07%ID/g. The coordination of Fe(III) and the amino group in the structure can be used for magnetic resonance imaging (MRI), whereas absorption in the near-infrared region can be applied to photoacoustic imaging (PAI) and photothermal therapy (PTT). MRI and PAI accurately identified the location of the tumor, and based on the imaging data, laser irradiation was employed accurately. With a high photothermal conversion efficiency of 44.3%, the bladder tumor was completely resected without recurrence. Hematological analysis and histopathological analysis jointly confirmed the high level of safety of the experiment.
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http://dx.doi.org/10.1021/acsbiomaterials.1c01217DOI Listing
February 2022

Z-Scheme heterostructures for glucose oxidase-sensitized radiocatalysis and starvation therapy of tumors.

Nanoscale 2022 Feb 10;14(6):2186-2198. Epub 2022 Feb 10.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Although many semiconductor heterojunctions have been prepared to promote radiation-generated exciton separation for radiocatalysis therapy (RCT), most of them inevitably sacrifice the redox ability of radiation-generated electrons and holes. Herein, we design and construct BiOI/[email protected] nanosheets modified by amine-polyethylene glycol-folic acid and glucose oxidase for glucose oxidase-sensitized RCT and starvation therapy (ST) synergistic therapy of tumors. The unique Z-scheme energy level arrangement between BiOI and BiS can elevate the charge separation efficiency, as well as maximize the redox ability of radiation-generated electrons and holes, leading to the enhancement of the therapeutic efficacy of RCT. Since glucose oxidase can supply excess HO for RCT to produce ˙OH on one hand, but efficiently cut off the energy supply of tumor cells ST, on the other hand, our nanosheets exhibit superior tumor therapeutic efficacy to any single treatment benefiting from the cascade and synergy effects between RCT and ST.
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http://dx.doi.org/10.1039/d1nr07096gDOI Listing
February 2022

A two-year measurement of methane and nitrous oxide emissions from freshwater aquaculture ponds: Affected by aquaculture species, stocking and water management.

Sci Total Environ 2022 Mar 26;813:151863. Epub 2021 Nov 26.

Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China; Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.

Aquaculture ponds are of increasing worldwide concerns as critical sources of atmospheric methane (CH) and nitrous oxide (NO), but little is known about these gases emissions as affected by aquaculture species, stocking and water management in aquaculture ponds. Here, a two-year study was carried out to quantify CH and NO emissions from freshwater crab and fish aquaculture ponds in subtropical China. We further explored how the microbial functional genes [CH: mcrA and pmoA; NO: archaeal and bacterial amoA (AOA + AOB), nirS, nirK, nosZ] may drive CH and NO release in the crab aquaculture pond typically undergoing flooding-to-drainage alteration. Over the two-year period, annual CH and NO fluxes averaged 0.95 mg m h and 20.94 μg m h in the fish aquaculture, and 0.78 mg m hand 28.48 μg m h in the crab aquaculture, respectively. The direct NO emission factors were estimated to be 0.77% and 0.36% of the total N input by feed or 1.59 g NO-N kg and 1.06 g NO-N kg aquaculture yield in the crab and fish ponds, respectively. Among three functional stocking areas, CH and NO emissions were consistently the highest at the feeding area (FA) in the both aquaculture ponds, followed by at the undisturbed area (UA) and aerated area (AA). The shift in sediment soil moisture from waterlogging to drainage conditions significantly increased the abundance of AOB relative to AOA and pmoA, decreased those of denitrifying functional genes (nirS, nirK, nosZ) and mcrA, while did not alter the functional group ratio of nirS + nirK relative to nosZ. Our results highlight that a better understanding of CH and NO emissions from aquaculture ponds requires taking into consideration of data sourced from more diverse aquaculture systems with different management patterns. In addition, a deep analysis of the microbial processes that drive CH and NO production and consumption from aquaculture ponds remains to be addressed in future studies.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151863DOI Listing
March 2022

Fe(III)-Shikonin Supramolecular Nanomedicine for Combined Therapy of Tumor via Ferroptosis and Necroptosis.

Adv Healthc Mater 2022 01 11;11(2):e2101926. Epub 2021 Nov 11.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.

Most of the antitumor chemotherapeutic drugs execute the therapeutic performance upon eliciting tumor cell apoptosis, which may cause chemoresistance of tumors. Design of novel drugs to eradicate apoptosis-resistant tumors via non-apoptotic cell death pathways is promising for improving the long-term chemotherapeutic efficacy. Herein, a Fe(III)-Shikonin metal-polyphenol-coordinated supramolecular nanomedicine for combined therapy of tumor via ferroptosis and necroptosis is designed. The construction of the nanomedicine based on the coordinated self-assembly between Fe and Shikonin not only overcomes the shortcomings of Shikonin including its low bioavailability and high toxicity toward normal tissues, but also integrates the theranostics functions of Fe ions. Under the exposure of the high concentration of glutathione (GSH) in tumor cells, the as-prepared nanomedicine will disassemble into Fe and Shikonin, followed by stimulating the tumor cell death through ferroptosis and necroptosis. In addition, benefiting from the stealth effect of polyethylene glycol (PEG) and the targeting ability of cyclo(Arg-Gly-Asp-d-Phe-Lys) (cRGD) to α β -integrin, NH -PEG-cRGD-modified nanomedicine exhibits a GSH-responsive therapy toward 4T1 tumor in vivo and self-enhanced longitudinal relaxation (T )-weighted imaging property. Since the self-assembly of natural Shikonin and human body-necessary Fe element is facile and feasible, the work may provide a promising supramolecular nanomedicine for next-generation chemotherapeutic applications.
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http://dx.doi.org/10.1002/adhm.202101926DOI Listing
January 2022

Data-driven estimates of fertilizer-induced soil NH , NO and N O emissions from croplands in China and their climate change impacts.

Glob Chang Biol 2022 02 17;28(3):1008-1022. Epub 2021 Nov 17.

Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Gaseous reactive nitrogen (Nr) emissions from agricultural soils to the atmosphere constitute an integral part of global N cycle, directly or indirectly causing climate change impacts. The extensive use of N fertilizer in crop production will compromise our efforts to reduce agricultural Nr emissions in China. A national inventory of fertilizer N-induced gaseous Nr emissions from croplands in China remains to be developed to reveal its role in shaping climate change. Here we present a data-driven estimate of fertilizer N-induced soil Nr emissions based on regional and crop-specific emission factors (EFs) compiled from 379 manipulative studies. In China, agricultural soil Nr emissions from the use of synthetic N fertilizer and manure in 2018 are estimated to be 3.81 and 0.73 Tg N yr , with a combined contribution of 23%, 20% and 15% to the global agricultural emission total of ammonia (NH ), nitrous oxide (N O) and nitric oxide (NO), respectively. Over the past three decades, NH volatilization from croplands has experienced a shift from a rapid increase to a decline trend, whereas N O and NO emissions always maintain a strong growth momentum due to a robust and continuous rise of EFs. Regionally, croplands in Central south (1.51 Tg N yr ) and East (0.99 Tg N yr ) of China exhibit as hotspots of soil Nr emissions. In terms of crop-specific emissions, rice, maize and vegetable show as three leading Nr emitters, together accounting for 61% of synthetic N fertilizer-induced Nr emissions from croplands. The global warming effect derived from cropland N O emissions in China was found to dominate over the local cooling effects of NH and NO emissions. Our established regional and crop-specific EFs for gaseous Nr forms provide a new benchmark for constraining the IPCC Tier 1 default EF values. The spatio-temporal insight into soil Nr emission data from N fertilizer application in our estimate is expected to advance our efforts towards more accurate global or regional cropland Nr emission inventories and effective mitigation strategies.
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http://dx.doi.org/10.1111/gcb.15975DOI Listing
February 2022

Positive and Negative Supervisor Development Feedback, Team Harmonious Innovation Passion and Team Creativity.

Front Psychol 2021 2;12:681910. Epub 2021 Sep 2.

Department of Accounting, School of Business, The Hong Kong University of Science and Technology, Kowloon, Hong Kong.

In modern organizations, creative work is usually carried out by teams, and the study of team creativity will therefore have meaningful implications for organization innovation research. The improvement of team creativity is a key management challenge for organization leaders. But our knowledge of how teams respond to and benefit from the supervisor's developmental feedback is limited. This paper draws on the interdependence and knowledge density of team creativity to study how the supervisor's developmental feedback influences creativity at the team level. Our statistical analysis of 94 supervisors and 330 employees finds that positive and negative development feedback from the supervisor both have a positive impact on team creativity, the impact from the negative development feedback is even stronger, and we also finds that the team's harmonious innovation passion mediates the relationship between the supervisor's developmental feedback and team creativity. In addition, we conclude that proactive personality activity moderates the relationship between the supervisor's positive (negative) developmental feedback and the team's harmonious innovation passion. Our research promotes the development of the study of team creativity in the Chinese cultural context and it is also an important application of developmental feedback that can be incorporated into management practices to enhance team creativity.
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http://dx.doi.org/10.3389/fpsyg.2021.681910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444987PMC
September 2021

Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration.

Int J Oral Sci 2021 08 18;13(1):27. Epub 2021 Aug 18.

Department of Oral Pathology, Hospital of Stomatology, Jilin University, Changchun, China.

Nanomaterial-based drug sustainable release systems have been tentatively applied to bone regeneration. They, however, still face disadvantages of high toxicity, low biocompatibility, and low drug-load capacity. In view of the low toxicity and high biocompatibility of polymer nanomaterials and the excellent load capacity of hollow nanomaterials with high specific surface area, we evaluated the hollow polydopamine nanoparticles (HPDA NPs), in order to find an optimal system to effectively deliver the osteogenic drugs to improve treatment of bone defect. Data demonstrated that the HPDA NPs synthesized herein could efficiently load four types of osteogenic drugs and the drugs can effectively release from the HPDA NPs for a relatively longer time in vitro and in vivo with low toxicity and high biocompatibility. Results of qRT-PCR, ALP, and alizarin red S staining showed that drugs released from the HPDA NPs could promote osteogenic differentiation and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) in vitro. Image data from micro-CT and H&E staining showed that all four osteogenic drugs released from the HPDA NPs effectively promoted bone regeneration in the defect of tooth extraction fossa in vivo, especially tacrolimus. These results suggest that the HPDA NPs, the biodegradable hollow polymer nanoparticles with high drug load rate and sustainable release ability, have good prospect to treat the bone defect in future clinical practice.
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http://dx.doi.org/10.1038/s41368-021-00132-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373924PMC
August 2021

Parametric characteristics analysis of three cells in 3D and five-directional annular braided composites.

PLoS One 2021 4;16(8):e0254691. Epub 2021 Aug 4.

Department of Industrial Design, Xi'an University of Technology, Xi'an, China.

On the basis of analyzing the movement law of 3D circular braided yarn, the three-cell model of 3D five-direction circular braiding composite material is established. By analyzing the node position relationship in various cell models, the calculation formulas of braiding angle, cell volume, fiber volume and fiber volume content in various cell models are obtained. It is found that there are four different braiding angles in four internal cells, and the braiding angles in internal cells gradually increase from inside to outside. The braiding angles of upper and lower surface cells are approximately equal. With the increase of the length of the knuckles, the braiding angles of each cell decrease, and the braiding angles of the four inner cells decrease greatly, while the braiding angles of upper and lower surfaces decrease slightly. The results of parametric analysis showed that with the increase of the length of the knuckles and the inner diameter of cells, the mass of cells increased proportionally, while the total fiber volume content of cells decreased. With the increase of braiding yarn number and axial yarn number, the unit cell mass decreases in direct proportion, and the unit cell total fiber volume content increases. Through the research results of this paper, the geometrical characteristics of the cell model under different braided parameters can be obtained, which greatly improves the analysis efficiency.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254691PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336883PMC
November 2021

Decreased Methane Emissions Associated with Methanogenic and Methanotrophic Communities in a Pig Manure Windrow Composting System under Calcium Superphosphate Amendment.

Int J Environ Res Public Health 2021 06 9;18(12). Epub 2021 Jun 9.

Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.

With the rapid growth of livestock breeding, manure composting has evolved to be an important source of atmospheric methane (CH) which accelerates global warming. Calcium superphosphate (CaSSP), as a commonly used fertilizer, was proposed to be effective in reducing CH emissions from manure composting, but the intrinsic biological mechanism remains unknown. Methanogens and methanotrophs both play a key role in mediating CH fluxes, therefore we hypothesized that the CaSSP-mediated reduction in CH emissions was attributed to the shift of methanogens and methanotrophs, which was regulated by physicochemical parameter changes. To test this hypothesis, a 60-day pig manure windrow composting experiment was conducted to investigate the response of CH emissions to CaSSP amendment, with a close linkage to methanogenic and methanotrophic communities. Results showed that CaSSP amendment significantly reduced CH emissions by 49.5% compared with the control over the whole composting period. The decreased gene (encodes the α-subunit of methyl-coenzyme M reductase) abundance in response to CaSSP amendment suggested that the CH emissions were reduced primarily due to the suppressed microbial CH production. Illumina MiSeq sequencing analysis showed that the overall distribution pattern of methanogenic and methanotrophic communities were significantly affected by CaSSP amendment. Particularly, the relative abundance of that is known to be a dominant group for CH production, significantly decreased by up to 25.3% accompanied with CaSSP addition. Only Type I methanotrophs was detected in our study and was the dominant methanotrophs in this composting system; in detail, CaSSP amendment increased the relative abundance of OTUs belong to and . Moreover, the increased SO concentration and decreased pH acted as two key factors influencing the methanogenic and methanotrophic composition, with the former has a negative effect on methanogenesis growth and can later promote CH oxidation at a low level. This study deepens our understanding of the interaction between abiotic factors, function microbiota and greenhouse gas (GHG) emissions, as well as provides implication for practically reducing composting GHG emissions.
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http://dx.doi.org/10.3390/ijerph18126244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296093PMC
June 2021

Morphological Development Trajectory and Structural Covariance Network of the Human Fetal Cortical Plate during the Early Second Trimester.

Cereb Cortex 2021 08;31(10):4794-4807

Laboratory of Neuro Imaging (LONI), USC Steven Neuroimaging and Informatics Institute, Keck School of Medicine of University of Southern California, Los Angeles, CA 90033, USA.

During the early second trimester, the cortical plate, or "the developing cortex", undergoes immensely complex and rapid development to complete its major complement of neurons. However, morphological development of the cortical plate and the precise patterning of brain structural covariance networks during this period remain unexplored. In this study, we used 7.0 T high-resolution magnetic resonance images of brain specimens ranging from 14 to 22 gestational weeks to manually segment the cortical plate. Thickness, area expansion, and curvature (i.e., folding) across the cortical plate regions were computed, and correlations of thickness values among different cortical plate regions were measured to analyze fetal cortico-cortical structural covariance throughout development of the early second trimester. The cortical plate displayed significant increases in thickness and expansions in area throughout all regions but changes of curvature in only certain major sulci. The topological architecture and network properties of fetal brain covariance presented immature and inefficient organizations with low degree of integration and high degree of segregation. Altogether, our results provide novel insight on the developmental patterning of cortical plate thickness and the developmental origin of brain network architecture throughout the early second trimester.
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http://dx.doi.org/10.1093/cercor/bhab123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408502PMC
August 2021

LncRNA GHET1 Promotes Hypoxia-Induced Glycolysis, Proliferation, and Invasion in Triple-Negative Breast Cancer Through the Hippo/YAP Signaling Pathway.

Authors:
Yu Wang Shuwei Liu

Front Cell Dev Biol 2021 1;9:643515. Epub 2021 Apr 1.

Research Center for Sectional and Imaging Anatomy Cheeloo College of Medicine, Shandong University, Jinan, China.

Objective: This study was to assess the specific impacts and mechanism of lncRNA GHET1 in the development of triple-negative breast cancer (TNBC).

Methods: The lncRNA GHET1 expression in TNBC tissues and adjacent healthy tissues was detected by qRT-PCR, and its expression was then measured at the cellular level, including TNBC cells and human normal breast epithelial cell line MCF10A. On the completion of transfection of negative shRNA or lncRNA GHET1 shRNA, the TNBC cells, HCC1937 and MDA-MB-468, were then cultured in a normoxia or hypoxia environment, respectively. 5-Ethynyl-2'-deoxyuridine (EdU) assay, colony formation assay, and transwell assay were applicable to the determination of cell proliferation, cell viability, and invasion in each group, respectively. Reagent kits were used for testing glucose consumption and lactate production levels. HCC1937 cells with knockdown or overexpression of lncRNA GHET1 were injected into the nude mice, followed by the examination of resulting tumor volume and weight. The distribution and expression of Hippo/YAP signaling pathway-related proteins were probed using western blotting.

Results: Highly expressed lncRNA GHET1 in TNBC tissues and cells and induction of lncRNA GHET1 by hypoxia were proved. Knockdown of lncRNA GHET1 significantly reduced proliferation, viability, and invasion of TNBC cells, and decreased glucose consumption and lactate production levels under the hypoxia condition. Furthermore, lncRNA GHET1 knockdown decreased HIF-1α expression in hypoxia and significantly inhibited tumor development . Knockdown of lncRNA GHET1 increased the phosphorylation levels of LATS1 and Yes-associated protein (YAP) to retain YAP within the cytoplasm, while the overexpression of lncRNA GHET1 or hypoxia promoted nuclear translocation of YAP and TNBC development.

Conclusion: LncRNA GHET1 expression can be induced by hypoxia, which leads to excessive activation of the Hippo/YAP signaling pathway, thus promoting TNBC progression.
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http://dx.doi.org/10.3389/fcell.2021.643515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047212PMC
April 2021

BiVO/[email protected] superparticles for tumor multimodal imaging and synergistic therapy.

J Nanobiotechnology 2021 Mar 29;19(1):90. Epub 2021 Mar 29.

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130012, People's Republic of China.

Background: Despite tremendous progress has been achieved in tumor theranostic over the past decade, accurate identification and complete eradication of tumor cells remain a great challenge owing to the limitation of single imaging modality and therapeutic strategy.

Results: Herein, we successfully design and construct BiVO/[email protected] (PDA) superparticles (SPs) for computed tomography (CT)/photoacoustic (PA)/magnetic resonance (MR) multimodal imaging and radiotherapy (RT)/photothermal therapy (PTT) synergistic therapy toward oral epithelial carcinoma. On the one hand, BiVO NPs endow BiVO/[email protected] SPs with impressive X-ray absorption capability due to the high X-ray attenuation coefficient of Bi, which is beneficial for their utilization as radiosensitizers for CT imaging and RT. On the other hand, FeO NPs impart BiVO/[email protected] SPs with the superparamagnetic property as a T-weighted contrast agent for MR imaging. Importantly, the aggregation of FeO NPs in SPs and the presence of PDA shell greatly improve the photothermal conversion capability of SPs, making BiVO/[email protected] SPs as an ideal photothermal transducer for PA imaging and PTT. By integrating advantages of various imaging modalities (CT/PA/MR) and therapeutic strategies (RT/PTT), our BiVO/[email protected] SPs exhibit the sensitive multimodal imaging feature and superior synergistic therapeutic efficacy on tumors.

Conclusions: Since there are many kinds of building blocks with unique properties appropriating for self-assembly, our work may largely enrich the library of nanomateirals for tumor diagnosis and treatment.
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http://dx.doi.org/10.1186/s12951-021-00802-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008624PMC
March 2021

Virasoro Hair and Entropy for Axisymmetric Killing Horizons.

Phys Rev Lett 2020 Dec;125(24):241302

Perimeter Institute for Theoretical Physics, 31 Caroline Street N, Waterloo, Ontario N2L 2Y5, Canada.

We show that the gravitational phase space for the near-horizon region of a bifurcate, axisymmetric Killing horizon in any dimension admits a 2D conformal symmetry algebra with central charges proportional to the area. This extends the construction of Haco et. al. [J. High Energy Phys. 12 (2018) 098JHEPFG1029-847910.1007/JHEP12(2018)098] to generic Killing horizons appearing in solutions of Einstein's equations and motivates a holographic description in terms of a 2D conformal field theory. The Cardy entropy in such a field theory agrees with the Bekenstein-Hawking entropy of the horizon, suggesting a microscopic interpretation. A set of appendixes is included in the Supplemental Material that provides examples and further details of the calculations presented in the main text.
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http://dx.doi.org/10.1103/PhysRevLett.125.241302DOI Listing
December 2020

Morphological changes in the central sulcus of children with isolated growth hormone deficiency versus idiopathic short stature.

Dev Neurobiol 2021 01 14;81(1):36-46. Epub 2020 Dec 14.

Research Center for Sectional and Imaging Anatomy, Shandong University Cheeloo College of Medicine, Shandong, China.

In this study, the morphological changes in the central sulcus between children with isolated growth hormone deficiency (IGHD) and those with idiopathic short stature (ISS) were analyzed. Thirty children with IGHD (peak growth hormone < 5 µg/L) and 30 children with ISS (peak growth hormone > 10.0 µg/L) were included. Morphological measurements of the central sulcus were obtained from T1-weighted MRIs using BrainVISA, including the average sulcal width, maximum depth, average depth, top length, bottom length, and depth position-based profiles (DPPs). The bilateral average width of the central sulci was significantly wider, while the left maximum depth and right average depth of the central sulcus were significantly smaller, in children with IGHD than in children with ISS. There were no significant differences in the right maximum depth, left average depth, or bilateral top length and bottom length of the central sulcus between groups. The DPPs of the middle part of both central sulci (corresponding to the hand motor activation area) and the inferior part of the right central sulcus (corresponding to the oral movement area) near the Sylvian fissure were significantly smaller in children with IGHD than in controls before false discovery rate (FDR) correction. However, all the above significant DPP sites disappeared after FDR correction. There were significant morphological changes in the three-dimensional structure of the central sulcus in children with IGHD, which were the outcome of other more essential cortical or subcortical changes, resulting in their relatively slower development in motor, cognitive, and linguistic functional performance.
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http://dx.doi.org/10.1002/dneu.22797DOI Listing
January 2021

Maize SRO1e represses anthocyanin synthesis through regulating the MBW complex in response to abiotic stress.

Plant J 2021 02 12;105(4):1010-1025. Epub 2020 Dec 12.

Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.

Plants experiencing abiotic stress react by generating reactive oxygen species (ROS), compounds that, if allowed to accumulate to excess, repress plant growth and development. Anthocyanins induced by abiotic stress are strong antioxidants that neutralize ROS, whereas their over-accumulation retards plant growth. Although the mechanism of anthocyanin synthesis has been revealed, how plants balance anthocyanin synthesis under abiotic stress to maintain ROS homeostasis is unknown. Here, ROS-related proteins, SIMILAR TO RCD-ONEs (SROs), were analysed in Zea mays (maize), and all six SRO1 genes were inducible by a variety of abiotic stress agents. The constitutive expression of one of these genes, ZmSRO1e, in maize as well as in Arabidopsis thaliana increased the sensitivity of the plant to abiotic stress, but repressed anthocyanin biosynthesis and ROS scavenging activity. Loss-of-function mutation of ZmSRO1e enhanced ROS tolerance and anthocyanin accumulation. We showed that ZmSRO1e competed with ZmR1 (a core basic helix-loop-helix subunit of the MYB-bHLH-WD40 transcriptional activation complex) for binding with ZmPL1 (a core MYB subunit of the complex). Thus, during the constitutive expression of ZmSRO1e, the formation of the complex was compromised, leading to the repression of genes, such as ZmA4 (encoding dihydroflavonol reductase), associated with anthocyanin synthesis. Overall, the results have revealed a mechanism that allows the products of maize SRO1e to participate in the abiotic stress response.
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http://dx.doi.org/10.1111/tpj.15083DOI Listing
February 2021

A Flexible Polymer Nanofiber-Gold Nanoparticle Composite Film for Solar-Thermal Seawater Desalination.

Macromol Rapid Commun 2020 Dec 16;41(24):e2000390. Epub 2020 Nov 16.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130021, P. R. China.

Interfacial solar-thermal conversion has broad application prospects in solar driven steam generation, seawater desalination, sewage purification, and other fields. For a wide range of applications, high-efficiency interfacial solar-thermal conversion materials with the feature of being lightweight, flexible, and easy to scale up at the same time are significantly valued. Herein, a strategy for the preparation of solar-thermal poly (4-vinylpyridine) (P4VP) nanofiber-gold nanoparticle (Au NP) composite film (PGCF) is reported. Combining with the flexible and lightweight P4VP nanofibers, these absorbed Au NPs enable better solar-thermal conversion efficiency. Accordingly, the PGCF provides high-performance interfacial solar-driven steam generation, with 77% solar-heat conversion efficiency under the power density of 3.4 kW m , which shows stable output (3.4 kg m h ) in the application of solar-driven seawater desalination. In addition, PGCF is light in weight, flexible, and suitable for scalable commercial production, enabling PGCF broad application prospects in the field of light-to-heat conversion.
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http://dx.doi.org/10.1002/marc.202000390DOI Listing
December 2020
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