Publications by authors named "Shuting Yang"

86 Publications

Development and Validation of Nomogram Prediction Model for Postoperative Sleep Disturbance in Patients Undergoing Non-Cardiac Surgery: A Prospective Cohort Study.

Nat Sci Sleep 2021 24;13:1473-1483. Epub 2021 Aug 24.

Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou City, Jiangsu Province, People's Republic of China.

Purpose: To develop a risk prediction nomogram of postoperative sleep disturbance (PSD) in patients undergoing non-cardiac surgery.

Patients And Methods: Data on 881 consecutive patients who underwent non-cardiac surgery at the Affiliated Hospital of Xuzhou Medical University between June 2020 and April 2021 were prospectively collected. Of these, we randomly divided 881 non-cardiac patients into two groups, training cohort (n = 617) and validation cohort (n = 264) at the ratio of 7:3. Characteristic variables were selected based on the data of training cohort through least absolute shrinkage and selection operator (LASSO) regression. Multivariate logistic regression was used to identify the independent risk factors associated with PSD that then were incorporated into the nomogram. The predictive performance of the nomogram was measured by concordance index (C index), receiver operating characteristic (ROC) curve, and calibration with 1000 bootstrap samples to decrease the over-fit bias.

Results: PSD was found in 443 of 617 patients (71.8%) and 190 of 264 patients (72.0%) in the training and validation cohorts, respectively. The perioperative risk factors associated with PSD were female sex, anxiety, dissatisfaction of ward environment, absence of combined regional nerve block, postoperative nausea and vomiting (PONV), the longer duration stayed in post anesthesia care unit (PACU), the higher dose of midazolam and sufentanil, the higher postoperative numeric rating score for pain (NRS) score. Incorporating these 9 factors, the nomogram achieved good concordance indexes of 0.82 (95% confidence interval [CI], 0.78-0.85) and 0.80 (95% CI, 0.74-0.85) in predicting PSD in the training and validation cohorts, respectively, and obtained well-fitted calibration curves. The sensitivity and specificity (95% CIs) of the nomogram were calculated, resulting in sensitivity of 74.0% (70.0-78.2%) and 75.3% (68.4-81.7%) and specificity of 79.3% (72.5-85.2%) and 70.3% (58.4-80.7%) for the training and validation cohorts, respectively. Patients who had a nomogram score of less than 262 or 262 or greater were considered to have low or high risks of PSD presence, respectively.

Conclusion: The proposed nomogram achieved an optimal prediction of PSD in patients undergoing non-cardiac surgery. The risks for an individual patient to harbor PSD can be determined by this model, which can lead to a reasonable preventive and treatment measures.
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http://dx.doi.org/10.2147/NSS.S319339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403031PMC
August 2021

RGS12 is required for the maintenance of mitochondrial function during skeletal development.

Cell Discov 2020 Sep 1;6(1):59. Epub 2020 Sep 1.

Department of Basic and Translational Sciences, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA, USA.

Mitochondrial morphology and function are crucial for tissue homeostasis, such as for skeletal development, but the cellular and molecular mechanisms remain unclear. Here, we provide evidence that regulator of G-protein signaling 12 (RGS12) is present in the mitochondria of primary chondrocytes and cartilage tissues. Deletion of RGS12 in type II collagen-positive cells led to a significant decrease in mitochondrial number, membrane potential, and oxidative phosphorylation function. Mechanistically, RGS12 promoted the function of ATP5A as an enhancer of tyrosine phosphorylation. Mice with RGS12 deficiency in the chondrocyte lineage showed serious body retardation, decreased bone mass, and chondrocyte apoptosis due to the defective activity of ATP synthase. To our knowledge, this is the first report that RGS12 is required for maintaining the function of mitochondria, which may allow it to orchestrate responses to cellular homeostasis.
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http://dx.doi.org/10.1038/s41421-020-00190-wDOI Listing
September 2020

Case Report: Novel Variants Cause Cornelia de Lange Syndrome in Chinese Patients.

Front Genet 2021 30;12:699894. Epub 2021 Jul 30.

Department of Medical Genetics, National Health Commission Key Laboratory of Birth Defects for Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, China.

Cornelia de Lange syndrome (CdLS) is a genetic disorder characterized by multisystemic malformations. Mutation in the gene accounts for nearly 60% of the cases. This study reports the clinical and genetic findings of three cases of CdLS from unrelated Chinese families. Clinically, all the three cases were classified as classic CdLS based on the cardinal (distinctive facial features and limb malformations) and suggestive (developmental delay, growth retardation, microcephaly, hirsutism, etc.) manifestations. SNP array detected a novel heterozygous microdeletion of 0.2 Mb [arr[GRCh37]5p13.2(36848530_37052821) × 1] that spans the first 43 exons of in the fetus with nuchal translucency thickening in case 1. Whole-exome sequencing in family trios plus Sanger sequencing validation identified a heterozygous c.5566G>A (p.R1856G) mutation in the fetus with intrauterine growth retardation in case 2 and a novel heterozygous c.448dupA (p.S150Kfs23) mutation in the proband (an 8-month-old girl) in case 3. The cases presented in this study may serve as references for increasing our understanding of the mutation spectrum of in association with CdLS.
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http://dx.doi.org/10.3389/fgene.2021.699894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362598PMC
July 2021

Diagnostic potential and future directions of matrix metalloproteinases as biomarkers in gingival crevicular fluid of oral and systemic diseases.

Int J Biol Macromol 2021 Oct 30;188:180-196. Epub 2021 Jul 30.

State Key Laboratory of Oral Diseases, Department of Periodontology, National Clinical Research Center for Oral Diseases, West China, Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address:

Gingival crevicular fluid (GCF) is a physiological fluid and an inflammatory serum exudate derived from the gingival plexus of blood vessels and mixed with host tissues and subgingival plaque flows. In addition to proteins, GCF contains a diverse population of cells, including desquamated epithelial cells, cytokines, electrolytes, and bacteria from adjacent plaques. Recently, matrix metalloproteinases(MMPs), which are endopeptidases that are active against extracellular macromolecules, in GCF have been revealed as potential utility biomarkers for the diagnosis and follow-up of oral and systemic diseases, thereby facilitating the early evaluation of malignancy risk and the monitoring of disease progression and treatment response. Tissue inhibitors of metalloproteinases (TIMPs) are specific inhibitors of matrixins that participate in the regulation of local activities of MMPs in tissues. This review provides an overview of the latest findings on the diagnostic and prognostic values of MMPs and TIMPs in GCF of oral and systemic diseases, including periodontal disease, pulpitis, peri-implantitis and cardiovascular disease as well as the extraction, detection and analytical methods for GCF.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.165DOI Listing
October 2021

Prenatal case of Simpson-Golabi-Behmel syndrome with a de novo 370Kb-sized microdeletion of Xq26.2 compassing partial GPC3 gene and review.

Mol Genet Genomic Med 2021 Aug 22;9(8):e1750. Epub 2021 Jul 22.

Department of Medical Genetics, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, China.

Background: Simpson-Golabi-Behmel syndrome type 1 (SGBS1) is a rare X-linked recessive disorder characterized by pre- and postnatal overgrowth and a broad spectrum of anomalies including craniofacial dysmorphism, heart defects, renal, and genital anomalies. Due to the ultrasound findings are not pathognomonic for this syndrome, most clinical diagnosis of SGBS1 are made postnatally.

Methods: A pregnant woman with abnormal prenatal sonographic findings was advised to perform molecular diagnosis. Single nucleotide polymorphism array (SNP array) was performed in the fetus, and the result was validated with multiplex ligation-dependent probe amplification (MLPA) and real-time quantitative PCR (qPCR).

Results: The prenatal sonographic presented with increased nuchal translucency at 13 gestational weeks, and later at 21 weeks with cleft lip and palate, heart defect, increased amniotic fluid index and over growth. A de novo 370Kb-deletion covering the 5'-UTR and exon 1 of GPC3 gene was detected in the fetus by SNP array, which was subsequently confirmed by MLPA and qPCR.

Conclusion: The de novo 370Kb hemizygous deletion of 5'-UTR and exon 1 of GPC3 results in the SGBS1 of this Chinese family. Combination of ultrasound and genetics tests helped us effectively to diagnose the prenatal cases of SGBS1. Our findings also enlarge the spectrum of mutations in GPC3 gene.
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http://dx.doi.org/10.1002/mgg3.1750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404223PMC
August 2021

In Situ Gel Polymer Electrolyte with Inhibited Lithium Dendrite Growth and Enhanced Interfacial Stability for Lithium-Metal Batteries.

ACS Appl Mater Interfaces 2021 Jul 6;13(27):32486-32494. Epub 2021 Jul 6.

National & Local Engineering Laboratory for Motive Power and Key Materials, Henan Normal University, Xinxiang, Henan 453007, China.

The practical application of lithium-metal anodes in high-energy-density rechargeable lithium batteries is hindered by the uncontrolled growth of lithium dendrites and limited cycle life. An ether-based gel polymer electrolyte (GPE-H) is developed through in situ polymerization method, which has close contact with the electrode interface. Based on DFT calculations, it was confirmed that the cationic groups produced by polar solvent tris(1,1,1,3,3,3-hexafluoroisopropyl) (HFiP) initiate the ring-opening polymerization of DOL in the battery. As a result, GPE-H achieves considerable ionic conductivity (1.6 × 10 S cm) at ambient temperature, high lithium-ion transference number ( > 0.6) and an electrochemical stability window as high as 4.5 V. GPE-H can achieve up to 800 h uniform lithium plating/stripping at a current density of 1.65 mA cm in Li symmetrical batteries. Li-S and LiFePO batteries using this GPE-H have long cycle performances at ambient temperature and high Coulomb efficiency (CE > 99.2%). From the above, in situ polymerized GPE-H electrolytes are promising candidates for high-energy-density rechargeable lithium batteries.
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http://dx.doi.org/10.1021/acsami.1c07032DOI Listing
July 2021

Identification of Cilia in Different Mouse Tissues.

Cells 2021 Jun 29;10(7). Epub 2021 Jun 29.

Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues.
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http://dx.doi.org/10.3390/cells10071623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307782PMC
June 2021

Total Flavonoids of Petals Release -Butyl Hydroperoxide-Induced Oxidative Stress in BRL-3A Cells.

Oxid Med Cell Longev 2021 5;2021:5453047. Epub 2021 Jun 5.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065 Sichuan, China.

Antioxidant and hepatoprotective activities in vitro of saffron petals were examined in this study for better utilizing saffron ( L.) biowaste. Using the DPPH and ABTS radical scavenging method, we compared the antioxidant activity and the content of total flavonoid extracts from petals (TFESP), stamens (TFESS), and both saffron petals and stamens (TFEMS). The results showed that the antioxidant capacity and the flavonoid content of TFESP were higher than those of TFESS and TFEMS. Then, the hepatoprotective activity of TFESP was determined, and the silymarin was used as a positive control. The main components of TFESP were analysed by ultrahigh performance liquid chromatography (UPLC) photodiode array (PDA)/mass spectrometry (MS) and nuclear magnetic resonance (NMR). The result showed that (1) TFESP could release oxidative liver injury induced by -butyl hydroperoxide (t-BHP). (2) TFESP could reduce the accumulation of reactive oxygen species (ROS); enhance the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH); and then improve the total antioxidant capacity (T-AOC) in BRL-3A cells. (3) TFESP could enhance the expression of B-cell lymphoma-2 () and decrease the expression of and ; increase the expression of Kelch-like ECH-associated protein-1 (), (), superoxide dismutase, and heme oxygenase 1 (); and downregulate inducible nitric oxide synthase (), interleukin-6 (), and nuclear factor kappa B-9 (). (4) The main hepatoprotective component of TFESP was identified as kaempferol-3-o-sophoroside. The mechanism may be that kaempferol-3-o-sophoroside can protect t-BHP-induced cell injury by regulating the expression of antioxidant, antiapoptotic, and anti-inflammatory genes. Thus, saffron petals are a potential hepatoprotective resource worthy of development.
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http://dx.doi.org/10.1155/2021/5453047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203408PMC
June 2021

Sodium Sulfite-Induced Mast Cell Pyroptosis and Degranulation.

J Agric Food Chem 2021 Jul 30;69(27):7755-7764. Epub 2021 Jun 30.

College of Food science and Engineering, Jilin University, Changchun, Jilin 130062, People's Republic of China.

Sodium sulfite, a common food additive, has been proved to cause allergic reaction. Pyroptosis is an inflammatory form of programmed cell death with plasma membrane lysis. In this study, we found that sodium sulfite triggered pyroptosis, which depended on reactive oxygen species (ROS)/NOD-like receptor protein 3 (NLRP3) in RBL-2H3 mast cells. Sodium sulfite increased the generation of ROS and the expression of NLRP3, caspase-1, gasdermin D N-terminal (GSDMD-N), interleukin-1β (IL-1β), and interleukin-18 (IL-18). The ROS scavenger N-acetyl-L-carnosine (NAC) and the NLRP3 inhibitor MCC950 reversed these effects. Furthermore, using a lactate dehydrogenase kit, propidium iodide staining, scanning electron microscopy, colocalization of GSDMD-N with histamine, and neutral red staining, we found that sodium sulfite notably induced cell membrane rupture. Because β-Hexosaminidase and histamine play a key role in allergic reactions, we detected the release of β-Hexosaminidase and histamine. The data showed that the release of β-Hexosaminidase and histamine induced by sodium sulfite was increased with dose independence, which were inhibited after treatment with NAC or MCC950. Overall, evidence suggested that pyroptosis induced by sodium sulfite may rupture the cell membrane and result in degranulation of mast cells. Our study may provide new insights for the mechanism by which sodium sulfite induces mast cell death and sensitization.
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http://dx.doi.org/10.1021/acs.jafc.1c02436DOI Listing
July 2021

Efficient Synthesis of Icetexane Diterpenes and Apoptosis Inducing Effect by Upregulating BiP-ATF4-CHOP Axis in Colorectal Cells.

J Nat Prod 2021 07 25;84(7):2012-2019. Epub 2021 Jun 25.

Xiangya School of Pharmaceutical Science, Central South University, Changsha, 410013 Hunan, China.

We herein present an efficient and robust synthetic strategy toward 12 icetexane diterpenes and their derivatives, which features a PPh/DIAD-mediated rearrangement of the reduced carnosic acid derivative () to give (-)-barbatusol () in a regioselective and scalable way. MTT assay led to the identification of (+)-grandione () and (-)-demethylsalvicanol -quinone derivative () as highly cytotoxic agents against HCT-116, COLO-205, and Caco-2 cells. Interestingly, (+)-grandione () induced the HCT-116 cell apoptosis in a dose-dependent manner, which might be attributed to the upregulation of the BiP-ATF4-CHOP axis and promotion of the BiP-ATF4 interactions, thereby leading to endoplasmic reticulum (ER) stress. This work not only paves an efficient and scalable pathway to access icetexane diterpenes but also provides new leads for the development of anticolorectal agents with a unique mode of action.
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http://dx.doi.org/10.1021/acs.jnatprod.1c00310DOI Listing
July 2021

The Climate Response to Emissions Reductions Due to COVID-19: Initial Results From CovidMIP.

Geophys Res Lett 2021 Apr;48(8):e2020GL091883

Irish Centre for High-End Computing (ICHEC) I Trinity Technology & Enterprise Campus Dublin 2 Ireland.

Many nations responded to the corona virus disease-2019 (COVID-19) pandemic by restricting travel and other activities during 2020, resulting in temporarily reduced emissions of CO, other greenhouse gases and ozone and aerosol precursors. We present the initial results from a coordinated Intercomparison, CovidMIP, of Earth system model simulations which assess the impact on climate of these emissions reductions. 12 models performed multiple initial-condition ensembles to produce over 300 simulations spanning both initial condition and model structural uncertainty. We find model consensus on reduced aerosol amounts (particularly over southern and eastern Asia) and associated increases in surface shortwave radiation levels. However, any impact on near-surface temperature or rainfall during 2020-2024 is extremely small and is not detectable in this initial analysis. Regional analyses on a finer scale, and closer attention to extremes (especially linked to changes in atmospheric composition and air quality) are required to test the impact of COVID-19-related emission reductions on near-term climate.
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http://dx.doi.org/10.1029/2020GL091883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206678PMC
April 2021

Implementation of fragile X syndrome carrier screening during prenatal diagnosis: A pilot study at a single center.

Mol Genet Genomic Med 2021 Jul 31;9(7):e1711. Epub 2021 May 31.

Department of Medical Genetics & the Prenatal Diagnosis Center of Hunan Province, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, China.

Background: Fragile X syndrome (FXS) is the most common inherited form of intellectual disability. Prenatal screening of FXS allows for early identification and intervention. The present study explored the feasibility of FXS carrier screening during prenatal diagnosis for those who were not offered screening early in pregnancy or prior to conception.

Methods: Pregnant women to be offered amniotic fluid testing were recruited for the free voluntary carrier screening at a single center between August, 2017 and September, 2019. The number of CGG repeats in the 5' un-translated region of the fragile X mental retardation gene 1 (FMR1) was determined.

Results: 4286 of 7000 (61.2%) pregnant women volunteered for the screening. Forty (0.93%), five (0.11%), and three (0.07%) carriers for intermediate mutation (45-54 repeats), premutation (55-200 repeats) and full mutation (>200 repeats) of the FMR1 gene were identified respectively. None of the detected premutation alleles were inherited by the fetuses. Of the three full mutation carrier mothers, all had a family history and one transmitted a full mutation allele to her male fetus.

Conclusion: Implementation of FXS carrier screening during prenatal diagnosis may be considered for the need to increase screening for FXS.
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http://dx.doi.org/10.1002/mgg3.1711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372084PMC
July 2021

Type II collagen-positive embryonic progenitors are the major contributors to spine and intervertebral disc development and repair.

Stem Cells Transl Med 2021 May 25. Epub 2021 May 25.

Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Type II collagen-positive embryonic progenitors are the major contributors to spine and intervertebral disc development and repair has been removed because it was published by mistake. The article will be published on October 1, 2021.
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http://dx.doi.org/10.1002/sctm.20-0424DOI Listing
May 2021

Phylogenetic characteristics of HIV among female cross-border travelers in Yunnan province between 2003 and 2012.

J Med Virol 2021 08 24;93(8):5173-5176. Epub 2021 May 24.

Clinical lab, Care Center for International Travel Health in Yunnan, Kunming, Yunnan, China.

Sexual transmission is currently the main mode of transmission of the human immunodeficiency virus (HIV). In this study, 181 HIV-infected female cross-border travelers entering Yunnan province were recruited between 2003 and 2012. HIV RNAs were extracted from their frozen serum and gag-pol gene sequences were obtained for phylogenetic and recombination analyses. In total, 131 gag-pol gene sequences were obtained successfully, at a rate of 72.4%. The most prevalent subtypes were CRF01_AE, followed by CRF08_BC, subtypes B and C. The other four subjects were classified as undefined subtypes and other recombinants. The subtype distribution of intravenous drug users was significantly different from that of sexually transmitted infections and unknown groups. The genetic distances of subtype B, C, and CRF01_AE strains were all close to the reference sequences from Yunnan province and Southeast Asian countries. Gene diversity and cocirculation of multiple subtypes were observed in female cross-border travelers, and CRF01_AE was the dominant epidemic subtype. The advantages of these subtype preferences for sexual transmission were obvious in HIV infection and transmission among this population. Our findings also suggest that close attention should be given to the HIV infection status of the female migrant population. In addition, a description of their epidemic characteristics is significant for the surveillance and prevention of acquired immunodeficiency syndrome in the Yunnan province.
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http://dx.doi.org/10.1002/jmv.27079DOI Listing
August 2021

TAZ is required for chondrogenesis and skeletal development.

Cell Discov 2021 Apr 20;7(1):26. Epub 2021 Apr 20.

Department of Basic & Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Chondrogenesis is a major contributor to skeletal development and maintenance, as well as bone repair. Transcriptional coactivator with PDZ-binding motif (TAZ) is a key regulator of osteogenesis and adipogenesis, but how TAZ regulates chondrogenesis and skeletal development remains undefined. Here, we found that TAZ expression is gradually increased during chondrogenic differentiation. Deletion of TAZ in chondrocyte lineage impaired articular and growth plate, as well as the bone development in TAZ-deficient mice. Consistently, loss of TAZ impaired fracture healing. Mechanistically, we found that ectopic expression of TAZ markedly promoted chondroprogenitor proliferation, while deletion of TAZ impaired chondrocyte proliferation and differentiation. TAZ associated with Sox5 to regulate the expression and stability of Sox5 and downstream chondrocyte marker genes' expression. In addition, overexpression of TAZ enhanced Col10a1 expression and promoted chondrocyte maturation, which was blocked by deletion of TAZ. Overall, our findings demonstrated that TAZ is required for skeletal development and joint maintenance that provided new insights into therapeutic strategies for fracture healing, heterotopic ossification, osteoarthritis, and other bone diseases.
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http://dx.doi.org/10.1038/s41421-021-00254-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058044PMC
April 2021

Macrophage regulator of G-protein signaling 12 contributes to inflammatory pain hypersensitivity.

Ann Transl Med 2021 Mar;9(6):448

Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Background: Pain is a predominant symptom in rheumatoid arthritis (RA) patients that results from joint inflammation and is augmented by central sensitization. Regulator of G-protein signaling 12 (RGS12) is the largest protein in the RGS protein family and plays a key role in the development of inflammation. This study investigated the regulation of RGS12 in inflammatory pain and explored the underlying mechanisms and potential RA pain targets.

Methods: Macrophage-specific RGS12-deficient (LysM-Cre;RGS12) mice were generated by mating RGS12 mice with LysM-Cre transgenic mice. Collagen antibody-induced arthritis (CAIA) models were induced in LysM-Cre;RGS12 mice by the administration of a cocktail of five monoclonal antibodies and LPS. Mouse nociception was examined using the von Frey and heat plate tests. Primary macrophages and RAW264.7 cells were used to analyze the regulatory function and mechanism of RGS12 . The expression and function of RGS12 and COX2 (cyclooxygenase 2) were determined by real-time PCR, ELISA, and luciferase assays.

Results: Ablation of RGS12 in macrophages decreased pain-related phenotypes, such as paw swelling, the clinical score, and the inflammatory score, in the CAIA model. LysM-Cre;RGS12 mice displayed increased resistance to thermal and mechanical stimulation from day 3 to day 9 during CAIA, indicating the inhibition of inflammatory pain. Overexpression of COX2 and PGE2 in macrophages enhanced RGS12 expression, and PGE2 regulated RGS12 expression through the G-protein-coupled receptors EP2 and EP4. Furthermore, RGS12 or the RGS12 PTB domain strengthened the transcriptional regulation of COX2 by NF-κB, whereas inhibiting NF-κB suppressed RGS12-mediated regulation of COX2 in macrophages.

Conclusions: Our results demonstrate that the deletion of RGS12 in macrophages attenuates inflammatory pain, which is likely due to impaired regulation of the COX2/PGE2 signaling pathway.
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http://dx.doi.org/10.21037/atm-20-5729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039686PMC
March 2021

Multifunctional organosilicon compound contributes to stable operation of high-voltage lithium metal batteries.

J Colloid Interface Sci 2021 Aug 17;595:35-42. Epub 2021 Mar 17.

National & Local Engineering Laboratory for Motive Power and Key Materials, College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China. Electronic address:

With the increasing demand for high-energy-density energy storage devices, lithium metal batteries have rekindled the interest of researchers due to ultra-high specific capacity. However, the extremely unstable interfaces between the electrolyte and electrodes limit its application seriously. Herein, we introduce an organosilicon compound, 1,3-Divinyltetramethyldisiloxane (DTMDS), as multifunctional electrolyte additive to enhance the performance of LiNiMnO/Li batteries. DTMDS contains two functional groups: siloxane groups (Si-O) and unsaturated carbon-carbon double bonds (CC). Siloxane groups can capture hydrogen fluoride (HF) in electrolyte, and the carbon-carbon double bonds can form thin and dense passivation layer on both cathode and anode surfaces by polymerization. As a result, the capacity retention of the batteries can retain more than 95% after 500 cycles. This work provides a valuable reference for the design of multifunctional additives and stabilizing the interfaces of high-voltage lithium metal batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.03.058DOI Listing
August 2021

Ego-Resiliency and Perceived Social Support in Late Childhood: A Latent Growth Modeling Approach.

Int J Environ Res Public Health 2021 03 14;18(6). Epub 2021 Mar 14.

Beijing Key Laboratory of Applied Experimental Psychology, National Demonstration Center for Experimental Psychology Education, Faculty of Psychology, Beijing Normal University, Beijing 100875, China.

This study explored the change trajectory of schoolchildren's ego-resiliency and perceived social support and investigated the effect of perceived social support on ego-resiliency across four time points. A sample of 437 children aged 8-13 years ( = 10.99, = 0.70, 51.5% boys) completed assessments at four time points. The results indicated that ego-resiliency showed an increasing linear trend and perceived social support showed a declining linear trend. Perceived social support had a positive effect on ego-resiliency over time. In addition, the initial status of perceived social support negatively predicted the growth trend of ego-resiliency, and the initial status of ego-resiliency negatively predicted the declining trend of perceived social support. The implications for theory and practice are discussed.
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http://dx.doi.org/10.3390/ijerph18062978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002070PMC
March 2021

Relationships Among Leaders' and Followers' Work Engagement and Followers' Subjective Career Success: A Multilevel Approach.

Front Psychol 2021 11;12:634350. Epub 2021 Mar 11.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Guangzhou, China.

Using a sample of 52 work teams (52 work team leaders and their 348 followers) in China, we investigated the influence mechanism of leaders' work engagement on their followers' work engagement and subjective career success. A multilevel structural equation model (MSEM) was applied to analyze the survey data. The results of this study indicated that leaders' work engagement positively influenced their followers' subjective career success, and this relationship was mediated by the followers' work engagement. Implications of these findings, limitations, and directions for future research are discussed in the final section of the paper.
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http://dx.doi.org/10.3389/fpsyg.2021.634350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991071PMC
March 2021

RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling.

Oncogene 2021 Apr 8;40(14):2553-2566. Epub 2021 Mar 8.

Department of Basic & Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Osteosarcoma (OS) is the most common primary malignancy of the bone that predominantly affects children and adolescents. Hippo pathway is a crucial regulator of organ size and tumorigenesis. However, how Hippo pathway regulates the occurrence of osteosarcoma is largely unknown. Here, we reported the regulator of G protein signaling protein 12 (RGS12) is a novel Hippo pathway regulator and tumor suppressor of osteosarcoma. Depletion of Rgs12 promotes osteosarcoma progression and lung metastasis in an orthotopic xenograft mouse model. Our data showed that the knockdown of RGS12 upregulates Ezrin expression through promoting the GNA12/13-RhoA-YAP pathway. Moreover, RGS12 negatively regulates the transcriptional activity of YAP/TEAD1 complex through its PDZ domain function to inhibit the expression and function of the osteosarcoma marker Ezrin. PDZ domain peptides of RGS12 can inhibit the development of intratibial tumor and lung metastases. Collectively, this study identifies that the RGS12 is a novel tumor suppressor in osteosarcoma through inhibiting YAP-TEAD1-Ezrin signaling pathway and provides a proof of principle that targeting RGS12 may be a therapeutic strategy for osteosarcoma.
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http://dx.doi.org/10.1038/s41388-020-01599-zDOI Listing
April 2021

Integrated CNV-seq, karyotyping and SNP-array analyses for effective prenatal diagnosis of chromosomal mosaicism.

BMC Med Genomics 2021 02 25;14(1):56. Epub 2021 Feb 25.

Department of Medical Genetics, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, 410008, Hunan, China.

Background: Emerging studies suggest that low-coverage massively parallel copy number variation sequencing (CNV-seq) more sensitive than chromosomal microarray analysis (CMA) for detecting low-level mosaicism. However, a retrospective back-to-back comparison evaluating accuracy, efficacy, and incremental yield of CNV-seq compared with CMA is warranted.

Methods: A total of 72 mosaicism cases identified by karyotyping or CMA were recruited to the study. There were 67 mosaic samples co-analysed by CMA and CNV-seq, comprising 40 with sex chromosome aneuploidy, 22 with autosomal aneuploidy and 5 with large cryptic genomic rearrangements.

Results: Of the 67 positive mosaic cases, the levels of mosaicism defined by CNV-seq ranged from 6 to 92% compared to the ratio from 3 to 90% by karyotyping and 20% to 72% by CMA. CNV-seq not only identified all 43 chromosomal aneuploidies or large cryptic genomic rearrangements detected by CMA, but also provided a 34.88% (15/43) increased yield compared with CMA. The improved yield of mosaicism detection by CNV-seq was largely due to the ability to detect low level mosaicism below 20%.

Conclusion: In the context of prenatal diagnosis, CNV-seq identified additional and clinically significant mosaicism with enhanced resolution and increased sensitivity. This study provides strong evidence for applying CNV-seq as an alternative to CMA for detection of aneuploidy and mosaic variants.
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http://dx.doi.org/10.1186/s12920-021-00899-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905897PMC
February 2021

[Application of chromosomal microarray analysis in prenatal diagnosis of pregnant women with advanced age].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Feb;38(2):101-107

Laboratory of Genetics, Lianyungang Maternal and Child Health Care Hospital, Lianyangang, Jiangsu 222000, China.

Objective: To assess the value of chromosomal microarray analysis (CMA) for the detection of fetal anomalies among pregnant women with advanced age.

Methods: CMA results of 562 cases, in addition with the outcome of pregnancy and neonatal follow-up were reviewed.

Results: Among the 562 amniotic fluid samples, 73 cases (12.99%) of fetal chromosomal abnormalities were detected, which included 21 cases (3.73%) of chromosomal aneuploidies and 52 cases (9.25%) of copy number variations (CNVs). The latters included 27 cases of pathological CNVs (4.80%), 4 cases of possible pathogenic CNVs (0.71%) and 42 cases of variants with unknown clinical significance (7.47%). Compared with those under 35, the detection rate of fetal chromosomal aneuploidies for women with advanced age was higher under the indications of voluntary test, abnormal ultrasonic structures, abnormal ultrasonic soft index and risks indicated by non-invasive prenatal testing (NIPT). No significant difference was found in the detection rate of CNVs between those ≥35 and <35 and between those with age factor only and with additional indications (P> 0.05). 552 cases (98.22%) of pregnant women have completed the followed up. Among 31 women with pathological and possible pathogenic fetal CNVs detected by CMA, 25 had terminated the pregnancy, 6 (19.35%) have delivered without obvious abnormality. 41 pregnant women with fetal CNVs of unknown clinical significance have completed the follow up, among whom 3 had terminated the pregnancy, 1 newborn was found with malformation after birth, which yielded an abnormal pregnancy rate of 9.76%. 480 pregnant women with negative CMA results have completed the follow up, among whom 5 (1.04%) had abnormal pregnancy or delivered a child with birth defect.

Conclusion: There is a certain difference between the outcome of pregnancy predicted by CMA testing and the actual outcome. The pregnancies with fetal CNVs with unknown clinical significance detected by CMA have a high adverse rate, which should attract clinical attention. CMA testing should be recommended for pregnant women with advanced age regardless of whether they have other symptoms. CMA combined with other detection methods is the trend for prenatal diagnosis.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200323-00195DOI Listing
February 2021

Whole genome sequencing reveals translocation breakpoints disrupting TP63 gene underlying split hand/foot malformation in a Chinese family.

Mol Genet Genomic Med 2021 03 20;9(3):e1604. Epub 2021 Jan 20.

Department of Medical Genetics, National Health Commission Key Laboratory of Birth Defects Research, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, China.

Background: Split hand/foot malformation (SHFM) is a congenital limb developmental disorder, which impairs the fine activities of hand/foot in the affected individuals seriously. SHFM is commonly inherited as an autosomal dominant disease with incomplete penetrance. Chromosomal aberrations such as copy number variations and translocations have been linked to SHFM. This study aimed to identify the genetic cause for three patients with bilateral hand and foot malformation in a Chinese family.

Methods: Karyotyping, single-nucleotide polymorphism (SNP) array, whole exome sequencing, whole genome sequencing, and Sanger sequencing were applied to identify the pathogenic variant.

Results: Karyotyping revealed that the three patients had balanced reciprocal translocation, 46, XX, t(3;15) (q29;q22). SNP array identified no pathogenic copy number variation in the proband. Trio-WES (fetus-mother-father) sequencing results revealed no pathogenic variants in the genes related to SHFM. Whole-genome low-coverage mate-pair sequencing (WGL-MPS), breakpoint PCR, and Sanger sequencing identified the breakpoints disrupting TP63 in the patients, but not in healthy family members.

Conclusion: This study firstly reports that a translocation breakpoint disrupting TP63 contributes to the SHFM in a Chinese family, which expands our knowledge of genetic risk and counseling underlying SHFM. It provides a basis for genetic counseling and prenatal diagnosis (preimplantation genetic diagnosis) for this family.
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http://dx.doi.org/10.1002/mgg3.1604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104154PMC
March 2021

Core-shell [email protected] nanoparticles with a thin and uniform carbon coating as a promising anode material for rechargeable lithium-ion batteries.

Dalton Trans 2021 Feb;50(5):1703-1711

School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453003, P. R. China.

Transition metal phosphides are used as anode materials for lithium-ion batteries because of their high theoretical capacity and low polarization. In this work, a core-shell [email protected] nanocomposite was successfully synthesized by a simple chemical vapor deposition (CVD) method, utilizing commercial GaP as the raw material and xylene as the carbon source. The uniform thin carbon shell could alleviate the volumetric variation and improve the conductivity of the inner GaP. When used as an anode in lithium-ion batteries, the [email protected] nanocomposite has a capacity of 812 mA h g-1 at a current density of 0.5 A g-1 after 100 cycles. At a high current density of 2 A g-1, the [email protected] anode delivers a good capacity value of 1087 mA h g-1.
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http://dx.doi.org/10.1039/d0dt04166aDOI Listing
February 2021

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Cell Metab 2020 12 12;32(6):1052-1062.e8. Epub 2020 Nov 12.

The Key Laboratory of Infection and Immunity of Shandong Province, Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan 250012, China. Electronic address:

Lipid accumulation in podocytes is a major determinant of diabetic kidney disease (DKD) and identification of potential therapeutic targets by mediating podocyte lipid metabolism has clinical importance. This study was to elucidate the role of JAML (junctional adhesion molecule-like protein) in the pathogenesis of DKD. We first confirmed the expression of JAML in podocytes and found that podocyte-specific deletion of Jaml ameliorated podocyte injury and proteinuria in two different models of diabetic mice. We further demonstrated a novel role of JAML in regulating podocyte lipid metabolism through SIRT1-mediated SREBP1 signaling. Similar results were also found in mice with adriamycin-induced nephropathy. Importantly, we observed a higher expression of JAML in glomeruli from subjects with DKD and other types of proteinuric kidney diseases, and the level of JAML was correlated with lipid accumulation and glomerular filtration rate, suggesting that JAML may be an attractive therapeutic target for proteinuric kidney disease.
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http://dx.doi.org/10.1016/j.cmet.2020.10.019DOI Listing
December 2020

Response to Letter to the Editor from Knobler et al: "Validation of the Swedish Diabetes Regrouping Scheme in Adult-Onset Diabetes in China".

J Clin Endocrinol Metab 2021 01;106(2):e1068-e1069

Department of Metabolism & Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, China.

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http://dx.doi.org/10.1210/clinem/dgaa828DOI Listing
January 2021

A novel strategy for improving performance of lithium-oxygen batteries.

J Colloid Interface Sci 2021 Feb 6;584:246-252. Epub 2020 Oct 6.

School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang City, Henan Province, 453007, PR China; National & Local Engineering Laboratory for Motive Power and Key Materials, Xinxiang 453000, PR China; Collaborative Innovation Center of Henan Province for Motive Power and Key Materials, Xinxiang 453000, PR China. Electronic address:

Although the theoretical energy density of lithium-oxygen batteries is extremely high, pulverization of lithium metal anode obviously influences batteries cycling performance. In this work, the cathode was coated with a membrane to protect the lithium anode from moisture attacking and avoid the pulverization. The membrane is composed of polyethylene oxide and poly tetra fluoroethylene, which improves the cycle life of the lithium-oxygen batteries cycles to 230 times, with a limited specific capacity of 1000 mAh·g, at a current density of 100 mA·g. Furthermore, the batteries perform stable charge and discharge cycles for 55 times in the air atmosphere, with the relative humidity greater than 50%. It demonstrates this strategy provides a new direction for the development of high-performance lithium-oxygen batteries.
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http://dx.doi.org/10.1016/j.jcis.2020.09.096DOI Listing
February 2021

Natural Frequency Response Evaluation for RC Beams Affected by Steel Corrosion Using Acceleration Sensors.

Sensors (Basel) 2020 Sep 17;20(18). Epub 2020 Sep 17.

College of Transportation, Jilin University, Changchun 130025, China.

This paper presented a laboratory investigation for analyzing the natural frequency response of reinforced concrete (RC) beams affected by steel corrosion. The electrochemical acceleration technique induced the corroded RC beams until the predetermined value of the steel corrosion ratio was achieved. Then, the natural frequency responses of the corroded beams were tested utilizing piezoelectric acceleration sensors. The damage states of the corroded beams were assessed through the measurement of crack parameters and the equivalent elastic modulus of the beams, which aims to clarify the fundamental characteristics of the dynamic response for the corroded RC beam with the increased steel corrosion ratio. The results revealed that steel corrosion reduces the bending stiffness of the RC beams and, thus, reduces the modal frequency. The variation of natural frequency can identify the corrosion damage even if no surface cracking of the RC beam, and the second-order frequency should be more indicative of the damage scenario. The degradations of stiffness and the natural frequency were estimated in this study by the free vibration equation of a simply supported beam, and a prediction method for the RC beam's residual service life was established. This study supports the use of variations in natural frequency as one diagnostic indicator to evaluate the health of RC bridge structures.
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http://dx.doi.org/10.3390/s20185335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570730PMC
September 2020

RGS12 is required for the maintenance of mitochondrial function during skeletal development.

Cell Discov 2020 1;6:59. Epub 2020 Sep 1.

Department of Basic and Translational Sciences, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA USA.

Mitochondrial morphology and function are crucial for tissue homeostasis, such as for skeletal development, but the cellular and molecular mechanisms remain unclear. Here, we provide evidence that regulator of G-protein signaling 12 (RGS12) is present in the mitochondria of primary chondrocytes and cartilage tissues. Deletion of RGS12 in type II collagen-positive cells led to a significant decrease in mitochondrial number, membrane potential, and oxidative phosphorylation function. Mechanistically, RGS12 promoted the function of ATP5A as an enhancer of tyrosine phosphorylation. Mice with RGS12 deficiency in the chondrocyte lineage showed serious body retardation, decreased bone mass, and chondrocyte apoptosis due to the defective activity of ATP synthase. To our knowledge, this is the first report that RGS12 is required for maintaining the function of mitochondria, which may allow it to orchestrate responses to cellular homeostasis.
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http://dx.doi.org/10.1038/s41421-020-00190-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459111PMC
September 2020

β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.

Am J Transl Res 2020 15;12(8):4757-4771. Epub 2020 Aug 15.

School of Medicine, Guangxi University Nanning, People's Republic of China.

Ischemia reperfusion (I/R) injury, an inevitable event accompanying heart transplantation, is the primary factor leading to organ failure and graft rejection. In order to prevent I/R injury, we established murine heart transplantation model with I/R and cell culture system to determine whether β-catenin is a mediate factor in preventing I/R injury in heart transplantation. After successfully established heterotopic heart transplantation mice model, the I/R injury was induced, and two dynamic temporal were studied during different I/R phases. With the increase of ischemia and reperfusion time, heart damage was more severe. In the initial study, we observed that β-catenin was significantly decreased, while ROCK1 and PTEN increased during the perfusion phase from day 0 to day 1, and remain the same level until 3 days later. The similar pattern that β-catenin was down-regulated while ROCK1 and PTEN were up-regulated was also observed in the dynamic temporal ischemia study. To further investigate the role of β-catenin signaling in I/R injury , β-catenin over-expressing plasmid was transfected into HL-1 cells, a cardiac cell line. We noted that β-catenin over-expressing cardiomyocytes showed decreased ROCK1/PTEN expression both at mRNA and protein levels. In addition, cobalt dichloride (CoCl) -induced oxidative stress model was further established to mimic cardiac I/R injury. We observed that CoCl-induced activation of ROCK1/PTEN signaling pathway were attenuated by transient transfection of a β-catenin over-expressing plasmid. Taken together, our results suggest that cardiac transplant induced IR injury is closely associated with the down-regulation of β-catenin and up-regulation of ROCK1 and PTEN expression.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476114PMC
August 2020
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