Publications by authors named "Shuqi Dong"

14 Publications

  • Page 1 of 1

Methionine-Based pH and Oxidation Dual-Responsive Block Copolymer: Synthesis and Fabrication of Protein Nanogels.

Biomacromolecules 2020 10 24;21(10):4063-4075. Epub 2020 Sep 24.

Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

In this paper, we synthesized a block copolymer containing pendent thioether functionalities by reversible addition-fragmentation chain transfer polymerization of a -butyloxycarbonyl (Boc)-l-methionine-(2-methacryloylethyl)ester (Boc-METMA) monomer using a poly(ethylene glycol) (PEG)-based chain transfer agent. The deprotection of Boc groups resulted in an oxidation and pH dual-responsive cationic block copolymer PEG--P(METMA). The block copolymer PEG--P(METMA) possessing protonable amine groups was water-soluble at pH < 6.0 and self-assembled to form spherical micelles at pH > 6.0. In the presence of HO, the micelles first became highly swollen with time and completely disassembled at last, demonstrating the HO-responsive feature because of the oxidation of hydrophobic thioether to hydrophilic sulfoxide. The anticancer drug curcumin (Cur) was entrapped in the polymeric micelles and the Cur-loaded micelles displayed a HO-triggered release profile as well as a pH-dependent release behavior, making PEG--P(METMA) micelles promising nanocarriers for reactive oxygen species-responsive drug delivery. Taking advantage of the protonated amine groups, the cationic polyelectrolyte PEG--P(METMA) formed polyion complex micelles with glucose oxidase (GOx) through electrostatic interactions at pH 5.8. By cross-linking the cores of PIC micelles with glutaraldehyde, the PIC micelles were fixed to generate stable GOx nanogels under physiological conditions. The GOx nanogels were glucose-responsive and exhibited glucose-dependent HO-generation activity and improved storage and thermal stability of GOx. Cur can be encapsulated in the GOx nanogels, and the Cur-loaded GOx nanogels demonstrate the glucose-responsive release profile. The GOx nanogels displayed high cytotoxicity to 4T1 cells and were effectively internalized by the cells. Therefore, these GOx nanogels have potential applications in the areas of cancer starvation and oxidation therapy.
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http://dx.doi.org/10.1021/acs.biomac.0c00879DOI Listing
October 2020

An investigation into the beneficial effects and molecular mechanisms of humic acid on foxtail millet under drought conditions.

PLoS One 2020 2;15(6):e0234029. Epub 2020 Jun 2.

Department of Agronomy, College of Agriculture, Shanxi Agricultural University, Taigu County, Jinzhong, Shanxi, China.

The aim of this study was to determine the effects and underlying molecular mechanisms of humic acid (HA) on foxtail millet (Setaria italica Beauv.) under drought conditions. The rainless climate of the Shanxi Province (37°42'N, 112°58'E) in China provides a natural simulation of drought conditions. Two foxtail millet cultivars, Jingu21 and Zhangza10, were cultivated in Shanxi for two consecutive years (2017-2018) based on a split-plot design. Plant growth, grain quality, and mineral elements were analyzed in foxtail millet treated with HA (50, 100, 200, 300, and 400 mg L-1) and those treated with clear water. Transcriptome sequencing followed by bioinformatics analysis was performed on plants in the normal control (CK), drought treatment (D), and drought + HA treatment (DHA) groups. Results were verified using real-time quantitative PCR (RT-qPCR). HA at a concentration of 100-200 mg L-1 caused a significant increase in the yield of foxtail millet and had a positive effect on dry weight and root-shoot ratio. HA also significantly increased P, Fe, Cu, Zn, and Mg content in grains. Moreover, a total of 1098 and 409 differentially expressed genes (DEGs) were identified in group D vs. CK and D vs. DHA, respectively. A protein-protein interaction network and two modules were constructed based on DEGs (such as SETIT_016654mg) between groups D and DHA. These DEGs were mainly enriched in the metabolic pathway. In conclusion, HA (100 mg L-1) was found to promote the growth of foxtail millet under drought conditions. Furthermore, SETIT_016654mg may play a role in the effect of HA on foxtail millet via control of the metabolic pathway. This study lays the foundation for research into the molecular mechanisms that underlie the alleviating effects of HA on foxtail millet under drought conditions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234029PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266348PMC
August 2020

Identification and evolution of C photosynthetic pathway genes in plants.

BMC Plant Biol 2020 Mar 30;20(1):132. Epub 2020 Mar 30.

College of Agronomy, Shanxi Agricultural University, Taigu, 030801, China.

Background: NADP-malic enzyme (NAPD-ME), and pyruvate orthophosphate dikinase (PPDK) are important enzymes that participate in C photosynthesis. However, the evolutionary history and forces driving evolution of these genes in C plants are not completely understood.

Results: We identified 162 NADP-ME and 35 PPDK genes in 25 species and constructed respective phylogenetic trees. We classified NADP-ME genes into four branches, A1, A2, B1 and B2, whereas PPDK was classified into two branches in which monocots were in branch I and dicots were in branch II. Analyses of selective pressure on the NAPD-ME and PPDK gene families identified four positively selected sites, including 94H and 196H in the a5 branch of NADP-ME, and 95A and 559E in the e branch of PPDK at posterior probability thresholds of 95%. The positively selected sites were located in the helix and sheet regions. Quantitative RT-PCR (qRT-PCR) analyses revealed that expression levels of 6 NADP-ME and 2 PPDK genes from foxtail millet were up-regulated after exposure to light.

Conclusion: This study revealed that positively selected sites of NADP-ME and PPDK evolution in C plants. It provides information on the classification and positive selection of plant NADP-ME and PPDK genes, and the results should be useful in further research on the evolutionary history of C plants.
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http://dx.doi.org/10.1186/s12870-020-02339-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106689PMC
March 2020

Comprehensive evaluation of fluroxypyr herbicide on physiological parameters of spring hybrid millet.

PeerJ 2019 26;7:e7794. Epub 2019 Sep 26.

Agronomy College, Shanxi Agricultural University, Taigu, China.

Foxtail millet ( L.) is an important food and fodder crop that is cultivated worldwide. Quantifying the effects of herbicides on foxtail millet is critical for safe herbicide application. In this study, we analyzed the effects of different fluroxypyr dosages on the growth parameters and physiological parametric of foxtail millet, that is, peroxidation characteristics, photosynthetic characteristics, and endogenous hormone production, by using multivariate statistical analysis. Indicators were screened via Fisher discriminant analysis, and the growth parameters, peroxidation characteristics, photosynthesis characteristics and endogenous hormones of foxtail millet at different fluroxypyr dosages were comprehensively evaluated by principal component analysis. On the basis of the results of principal component analysis, the cumulative contribution rate of the first two principal component factors was 93.72%. The first principal component, which explained 59.23% of total variance, was selected to represent the photosynthetic characteristics and endogenous hormones of foxtail millet. The second principal component, which explained 34.49% of total variance, represented the growth parameters of foxtail millet. According to the principal component analysis, the indexes were simplified into comprehensive index , and the mathematical model of comprehensive index was set as F = 0.592 + 0.345 . The results showed that the comprehensive evaluation score of fluroxypyr at moderate concentrations was higher than at high concentrations. Consequently, one L (active ingredient, ai) ha fluroxypyr exerted minimal effects on growth parameters, oxidase activity, photosynthetic activity, and endogenous hormones, and had highest value of comprehensive evaluation, which had efficient and safe benefits in foxtail millet field.
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http://dx.doi.org/10.7717/peerj.7794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766372PMC
September 2019

Comparative Genomics Analysis in Grass Species Reveals Two Distinct Evolutionary Strategies Adopted by R Genes.

Sci Rep 2019 07 24;9(1):10735. Epub 2019 Jul 24.

Agronomy College, Shanxi Agricultural University, Taigu, 030801, China.

Resistance genes play an important role in the defense of plants against the invasion of pathogens. In Setaria italica and closely related grass species, R genes have been identified through genetic mapping and genome-wide homologous/domain searching. However, there has been to date no systematic analysis of the evolutionary features of R genes across all sequenced grass genomes. Here, we determined and comprehensively compared R genes in all 12 assembled grass genomes and an outgroup species (Arabidopsis thaliana) through synteny and selection analyses of multiple genomes. We found that the two groups of nucleotide binding site (NBS) domains containing R genes-R tandem duplications (TD) and R singletons-adopted different strategies and showed different features in their evolution. Based on K/K analysis between syntenic R loci pairs of TDs or singletons, we conclude that R singletons are under stronger purifying selection to be conserved among different grass species than R TDs, while R genes located at TD arrays have evolved much faster through diversifying selection. Furthermore, using the variome datasets of S. italica populations, we scanned for selection signals on genes and observed that a part of R singleton genes have been under purifying selection in populations of S. italica, which is consistent with the pattern observed in syntenic R singletons among different grass species. Additionally, we checked the synteny relationships of reported R genes in grass species and found that the functionally mapped R genes for novel resistance traits are prone to appear in TDs and are heavily divergent from their syntenic orthologs in other grass species, such the black streak R gene Rxo1 in Z. mays and the blast R gene Pi37 in O. sativa. These findings indicate that the R genes from TDs adopted tandem duplications to evolve faster and accumulate more mutations to facilitate functional innovation to cope with variable threats from a fluctuating environment, while R singletons provide a way for R genes to maintain sequence stability and retain conservation of function.
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http://dx.doi.org/10.1038/s41598-019-47121-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656885PMC
July 2019

Macranthol attenuates lipopolysaccharide-induced depressive-like behaviors by inhibiting neuroinflammation in prefrontal cortex.

Physiol Behav 2019 05 10;204:33-40. Epub 2019 Feb 10.

Department of Chemical and Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, Fujian Province, PR China; Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, Fujian Province, PR China. Electronic address:

Macranthol is a lignans natural product isolated from Illicium dunnianum Tutch. Our previous studies have shown that BDNF dependent signaling pathway activation was involved in the antidepressant-like effects of macranthol. However, it is not clear whether neuro-inflammation suppression is involved in the effects of macranthol. Therefore, the aim of this present study was to determine whether macranthol affected the neuro-inflammation system in lipopolysaccharide (LPS)-induced mice by measuring pro-inflammatory cytokines and CD11b. Macranthol was orally administrated for successive seven days before a single LPS injection. The behavioral evaluation showed that macranthol prevented LPS-induced depressive-like deficits both in sucrose preference test and forced swimming test. The elevation of serum and prefrontal cortex pro-inflammatory cytokines including interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was decreased by macranthol pretreatment. In addition, LPS induced the elevation of CD11b in the prefrontal cortex, which was also inhibited by macranthol. Last but not the least, the immunofluorescence found that the number of positive iba-1 cells was also decreased by macranthol. These findings suggest that macranthol could alleviate depressive-like behaviors in mice induced by LPS that are mediated, at least by suppressing microglia-related neuro-inflammation in the prefrontal cortex.
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http://dx.doi.org/10.1016/j.physbeh.2019.02.010DOI Listing
May 2019

Polymer Brush Decorated MOF Nanoparticles Loaded with AIEgen, Anticancer Drug, and Supramolecular Glue for Regulating and In Situ Observing DOX Release.

Macromol Biosci 2018 12 17;18(12):e1800317. Epub 2018 Oct 17.

School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin, 300384, P. R. China.

UiO-66-based metal-organic framework nanoparticles functionalized with abundant polycationic segments as polymer brushes on surface are created to load negatively charged functional entities composed of anticancer drug, doxorubicin (DOX), and aggregation-induced emissive luminogen, a tetraphenylethene (TPE) derivative, followed by further modification with cucurbit[7]uril (CB[7]) as a supramolecular glue with the aim of pursuing a pH-responsive release function of the loaded functional components. The subsequent investigations verified the constructed formulation capable of promoting the stability and cellular uptake of the nanoparticles. The fluorescence resonance energy transfer between TPE donor and DOX acceptor enables in situ observation of pH-responsive DOX release. Significantly, CB modification appears to facilitate intracellular release of the DOX payload, eventually accounting for potent cytotoxicity to the treated cells.
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http://dx.doi.org/10.1002/mabi.201800317DOI Listing
December 2018

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet ( L.).

PeerJ 2018 8;6:e4752. Epub 2018 May 8.

College of Agronomy, Shanxi Agricultural University, Taigu, China.

Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet ( L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ∼20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contributed to the formation of a regulatory network involving multiple biological processes and pathways including photosynthesis, signal transduction, transcriptional regulation, redox regulation, hormonal signaling, and osmotic regulation. RNA-seq analysis also showed that some photosynthesis-related DEGs were highly expressed in M79 compared to its parental lines under drought stress. These results indicate that various molecular pathways, including photosynthesis, respond to drought stress in M79, and provide abundant molecular information for further analysis of the underlying mechanism responding to this stress.
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http://dx.doi.org/10.7717/peerj.4752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947103PMC
May 2018

Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice.

Int Immunopharmacol 2018 Jun 5;59:61-67. Epub 2018 Apr 5.

Department of Chemical and Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, Fujian Province, PR China.

Magnolol, the main constituent of Magnolia officinalis, has been shown to produce antidepressant-like effect in rodents. Growing evidence shows that neuroinflammation, oxidative stress and neuroendocrine contribute to the pathogenesis of major depression. Here, the aim of this present study was to determine whether magnolol affected these systems in mice exposed to chronic mild stress (CMS). The ameliorative effect of magnolol on depressive-like symptoms was investigated through behavioral tests, including the classical sucrose preference and forced swimming tests. The behavioral evaluation showed that magnolol reversed the depressive-like deficits both in sucrose preference test and forced swimming test. The elevation of prefrontal cortex pro-inflammatory cytokines such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was decreased by magnolol. Consistently, the microglia activation by CMS was also alleviated by magnolol. In addition, the hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis induced by CMS was attenuated by magnolol. Moreover, the increased lipid peroxidation such as malonaldehyde (MDA) and decreased antioxidant defense enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) induced by CMS were also reversed by magnolol. These findings suggest that administration of magnolol could alleviate depressive-like behaviors in CMS mice that are mediated by suppressing neuroinflammation and oxidative stress in the prefrontal cortex.
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http://dx.doi.org/10.1016/j.intimp.2018.03.031DOI Listing
June 2018

A dendritic catiomer with an MOF motif for the construction of safe and efficient gene delivery systems.

J Mater Chem B 2017 Nov 23;5(42):8322-8329. Epub 2017 Oct 23.

School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, P. R. China.

Ethanolamine (EA)-functionalized poly (glycidyl methacrylate) (PGMA-EA) has been validated by our pioneering studies as a promising material for the manufacture of gene delivery systems. Herein, PGMA-EA was schemed to attach at the terminus of MOF ligands for the construction of a nanoscaled dendritic catiomer. The resulting molecular structure was arranged to possess well-defined flanking secondary amine and hydroxyl groups, which consequently exerted drastic potency in DNA condensation (approximately 100 nm) and provided appreciable colloidal stabilities in the biological milieu. Note that as compared to the commercial gold standard of branched PEI (25 kDa), the proposed catiomer exhibited markedly higher transfection efficiency because of the appreciable cationic dendritic structure and lower cytotoxicity because of rational arrangement of hydroxyl groups. Therefore, the present study encouraged the utility of an MOF-motif in engineering spatial functional and biological functional nanoscaled structures for biomedical applications.
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http://dx.doi.org/10.1039/c7tb01966aDOI Listing
November 2017

Grain Yield and Quality of Foxtail Millet (Setaria italica L.) in Response to Tribenuron-Methyl.

PLoS One 2015 13;10(11):e0142557. Epub 2015 Nov 13.

Laboratory of Crop Chemical Regulation and Chemical Weed Control, College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi, the People's Republic of China.

Foxtail millet (Setaria italica L.) is cultivated around the world for human and animal consumption. There is no suitable herbicide available for weed control in foxtail millet fields during the post-emergence stage. In this study, we investigated the effect and safety of the post-emergence herbicide tribenuron-methyl (TBM) on foxtail millet in terms of grain yield and quality using a split-plot field design. Field experiments were conducted using two varieties in 2013 and 2014, i.e., high-yielding hybrid Zhangzagu 10 and high-quality conventional Jingu 21. TBM treatments at 11.25 to 90 g ai ha(-1) reduced root and shoot biomass and grain yield to varying degrees. In each of the two years, grain yield declined by 50.2% in Zhangzagu 10 with a herbicide dosage of 45 g ai ha(-1) and by 45.2% in Jingu 21 with a herbicide dosage of 22.5 g ai ha(-1) (recommended dosage). Yield reduction was due to lower grains per panicle, 1000-grain weight, panicle length, and panicle diameter. Grain yield was positively correlated with grains per panicle and 1000-grain weight, but not with panicles ha(-1). With respect to grain protein content at 22.5 g ai ha(-1,) Zhangzagu 10 was similar to the control, whereas Jingu 21 was markedly lower. An increase in TBM dosage led to a decrease in grain Mn, Cu, Fe, and Zn concentrations. In conclusion, the recommended dosage of TBM was relatively safe for Zhangzagu 10, but not for Jingu 21. Additionally, the hybrid variety Zhangzagu 10 had a greater tolerance to TBM than the conventional variety Jingu 21.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0142557PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643917PMC
July 2016

Photosynthetic physiological response of Radix Isatidis (Isatis indigotica Fort.) seedlings to nicosulfuron.

PLoS One 2014 28;9(8):e105310. Epub 2014 Aug 28.

Laboratory of Crop Chemical Regulation and Chemical Weed Control, Agronomy College, Shanxi Agricultural University, Taigu, the People's Republic of China.

Radix Isatidis (Isatis indigotica Fort.) is one of the most important traditional Chinese medicine plants. However, there is no suitable herbicide used for weed control in Radix Isatidis field during postemergence stage. To explore the safety of sulfonylurea herbicide nicosulfuron on Radix Isatidis (Isatis indigotica Fort.) seedlings and the photosynthetic physiological response of the plant to the herbicide, biological mass, leaf area, photosynthetic pigment content, photosynthetic rate, chlorophyll fluorescence characteristics, and P700 parameters of Radix Isatidis seedlings were analyzed 10 d after nicosulfuron treatment at 5th leaf stage in this greenhouse research. The results showed that biological mass, total chlorophyll, chlorophyll a, and carotenoids content, photosynthetic rate, stomatal conductance, PS II maximum quantum yield, PS II effective quantum yield, PS II electron transport rate, photochemical quenching, maximal P700 change, photochemical quantum yield of PS I, and PS I electron transport rate decreased with increasing herbicide concentrations, whereas initial fluorescence, quantum yield of non-regulated energy dissipation in PS II and quantum yield of non-photochemical energy dissipation due to acceptor side limitation in PS I increased. It suggests that nicosulfuron ≥1 mg L-1 causes the damage of chloroplast, PS II and PS I structure. Electron transport limitations in PS I receptor side, and blocked dark reaction process may be the main cause of the significantly inhibited growth and decreased photosynthetic rate of Radix Isatidis seedlings.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0105310PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148306PMC
October 2015

A case report of concurrent gastric and pulmonary mucosa-associated lymphoid tissue lymphomas and review of the literature on clonality analysis.

Ann Hematol 2013 Dec 24;92(12):1707-9. Epub 2013 Apr 24.

Department of Gastroenterology, Sir Run Run Shaw Affiliated Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang Province, China.

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http://dx.doi.org/10.1007/s00277-013-1749-6DOI Listing
December 2013

Induction of seed germination in Orobanche spp. by extracts of traditional Chinese medicinal herbs.

Sci China Life Sci 2012 Mar 14;55(3):250-60. Epub 2012 Apr 14.

College of Resources and Environment, Northwest A & F University, Yangling 712100, China.

The co-evolution of Orobanche spp. and their hosts within the same environment has resulted in a high degree of adaptation and effective parasitism whereby the host releases parasite germination stimulants, which are likely to be unstable in the soil. Our objective was to investigate whether extracts from non-host plants, specifically, Chinese medicinal plants, could stimulate germination of Orobanche spp. Samples of 606 Chinese medicinal herb species were extracted with deionized water and methanol. The extracts were used to induce germination of three Orobanche species; Orobanche minor, Orobanche cumana, and Orobanche aegyptiaca. O. minor exhibited a wide range of germination responses to the various herbal extracts. O. cumana and O. aegyptiaca exhibited an intermediate germination response to the herbal extracts. O. minor, which has a narrow host spectrum, showed higher germination rates in response to different herbal extracts compared with those of O. cumana and O. aegyptiaca, which have a broader host spectrum. Methanolic extracts of many Chinese herbal species effectively stimulated seed germination among the Orobanche spp., even though they were not the typical hosts. The effective herbs represent interesting examples of potential trap crops. Different countries can also screen extracts from indigenous herbaceous plants for their ability to induce germination of Orobanche spp. seeds. The use of such species as trap plants could diminish the global soil seed bank of Orobanche.
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http://dx.doi.org/10.1007/s11427-012-4302-2DOI Listing
March 2012
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