Publications by authors named "Shuo Yang"

759 Publications

Double-Layered Conductive Network Design of Flexible Strain Sensors for High Sensitivity and Wide Working Range.

ACS Appl Mater Interfaces 2022 Aug 4. Epub 2022 Aug 4.

Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science & Technology, Tianjin 300457, China.

For flexible strain sensors, the optimization between sensitivity and working range is a significant challenge due to the fact that high sensitivity and high working range are usually difficult to obtain at the same time. Herein, a breathable flexible strain sensor with a double-layered conductive network structure was designed and developed, which consists of a thermoplastic polyurethane (TPU)/carbon nanotube (CNT) layer (as a substrate layer) and a Ag nanowire (AgNW) layer. The TPU/CNT layer is made of electrospinning TPU with CNTs deposited onto the surface of TPU fibers, and the flexible TPU/CNT mat guarantees the integrity of the conductive path under a large strain. The AgNW layer was prepared by depositing different amounts of AgNWs on the surface of the TPU/CNT layer, and the high-conductivity AgNWs offer a low initial resistance. Benefitting from the synergistic two-layer structure, the as-obtained flexible strain sensor exhibits a very high sensitivity (up to 1477.7) and a very wide working range (up to 150%). Besides, the fabricated sensor exhibits fast response (88 ms), excellent dynamical stability (7000 cycles), and excellent breathability. The working mechanism of the strain sensor was further investigated using various techniques (microscopy, equivalent circuit, and thermal effects of current). Furthermore, the as-fabricated flexible strain sensors accurately detect the omnidirectional human motions, including subtle and large human motions. This work provides an efficient approach to achieve the optimization between high sensitivity and large working range of strain sensors, which may have great potential applications in health monitoring, body motion detection, and human-machine interactions.
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http://dx.doi.org/10.1021/acsami.2c08285DOI Listing
August 2022

Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3.

Front Vet Sci 2022 8;9:895190. Epub 2022 Jul 8.

College of Animal Sciences and Technology, Northeast Agricultural University, Harbin, China.

Chimeric RNA is a crucial target for tumor diagnosis and drug therapy, also having its unique biological role in normal tissues. (), a chimeric RNA discovered by our laboratory in porcine muscle tissue, can inhibit the proliferation of Porcine Skeletal Muscle Satellite Cells (PSCs). The regulatory mechanism of in PSCs remains unclear, but we speculate that and may be the target genes of . In this study, we explored the effects of and on cell viability and cell proliferation by CCK-8 assay, EdU staining and flow cytometry. Furthermore, the regulatory pathway of proliferation in PSCs mediated by through or was elucidated by co-transfection and co-immunoprecipitation (Co-IP). The results revealed that overexpression of significantly increased cell viability and the expression level of , and also promotes cell proliferation by changing cells from the G1 phase to the S phase. In addition, inhibiting the expression of substantially reduced cell viability and inhibited cell proliferation. Overexpression of and had no significant effect on cell viability and proliferation. Co-transfection experiments showed that could rescue the proliferation inhibition of PSCs caused by . Co-IP assay indicated that directly interacts with . Our study explores the hypothesis that directly regulates , indirectly regulating , thereby regulating PSCs proliferation. We provide new putative mechanisms of porcine skeletal muscle growth and lay the foundation for the study of chimeric RNA in normal tissues.
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http://dx.doi.org/10.3389/fvets.2022.895190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309209PMC
July 2022

Role of Glycolysis/Gluconeogenesis and HIF-1 Signaling Pathways in Rats with Dental Fluorosis Integrated Proteomics and Metabolomics Analysis.

Int J Mol Sci 2022 Jul 27;23(15). Epub 2022 Jul 27.

Department of Environmental Health, School of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China.

Fluoride is widely distributed, and excessive intake will lead to dental fluorosis. In this study, six offspring rats administrated 100 mg/L sodium fluoride were defined as the dental fluorosis group, and eight offspring rats who received pure water were defined as the control group. Differentially expressed proteins and metabolites extracted from peripheral blood were identified using the liquid chromatography tandem mass spectrometry and gas chromatography mass spectrometry, with the judgment criteria of fold change >1.2 or <0.83 and < 0.05. A coexpression enrichment analysis using OmicsBean was conducted on the identified proteins and metabolites, and a false discovery rate (FDR) < 0.05 was considered significant. Human Protein Atlas was used to determine the subcellular distribution of hub proteins. The Gene Cards was used to verify results. A total of 123 up-regulated and 46 down-regulated proteins, and 12 up-regulated and 2 down-regulated metabolites were identified. The significant coexpression pathways were the HIF-1 (FDR = 1.86 × 10) and glycolysis/gluconeogenesis (FDR = 1.14 × 10). The results of validation analysis showed the proteins related to fluorine were mainly enriched in the cytoplasm and extrinsic component of the cytoplasmic side of the plasma membrane. The HIF-1 pathway (FDR = 1.01 × 10) was also identified. Therefore, the HIF-1 and glycolysis/gluconeogenesis pathways were significantly correlated with dental fluorosis.
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http://dx.doi.org/10.3390/ijms23158266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332816PMC
July 2022

Pregnancy-related complications in systemic lupus erythematosus.

J Autoimmun 2022 Jul 21:102864. Epub 2022 Jul 21.

Department of Laboratory Medicine, Peking University Third Hospital, Beijing, 100191, China; Core Unit of National Clinical Research Center for Laboratory Medicine, Peking University Third Hospital, Beijing, 100191, China. Electronic address:

Systemic lupus erythematosus (SLE) is a systemic autoimmune inflammatory disease that predominantly affects women of childbearing age and results in various adverse pregnancy outcomes (APOs). Pregnancy was formerly discouraged in patients with SLE because of unstable disease activity during the gestation period, increased thrombosis risk, severe organ damage, and inevitable side effects of immunosuppressive agents. Currently, most patients with SLE have successful pregnancies due to preconception counselling, strict monitoring, and improved therapy with minimised complications for both the mother and foetus. Hydroxychloroquine (HCQ) is extensively used and is beneficial for improving pregnancy outcomes. However, pregnant women with SLE have a high-risk of APOs, such as disease flare, preterm birth, intrauterine growth restriction (IUGR), preeclampsia, and pregnancy loss. Better understanding of the changes in maternal immunity and serum biomarkers, as well as their relationships with SLE-related APOs progression, would facilitate the investigation of molecular mechanisms for triggering and ameliorating APOs. Furthermore, it would enable us to explore and develop novel and effective therapeutic strategies to prevent disease activation. Therefore, this review briefly introduces the interaction between pregnancy outcomes and SLE, elucidates pathophysiological and immunological changes during SLE pregnancy. Furthermore, this review systematically expounds on the effective predictors of APOs and the molecular mechanisms underlying the SLE-related APOs to provide a solid foundation for the advanced management of lupus pregnancy.
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http://dx.doi.org/10.1016/j.jaut.2022.102864DOI Listing
July 2022

Engineered chimeric insecticidal crystalline protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.).

Sci Rep 2022 Jul 22;12(1):12529. Epub 2022 Jul 22.

Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, China.

The insecticidal crystalline proteins (Crys) are a family of insect endotoxin functioning in crop protection. As insects keep evolving into tolerance to the existing Crys, it is necessary to discover new Cry proteins to overcome potential threatens. Crys possess three functional domains at their N-termini, and the most active region throughout evolution was found at the domain-III. We swapped domain-IIIs from various Cry proteins and generated seven chimeric proteins. All recombinants were expressed in Escherichia coli and their toxicity was assessed by dietary exposure assays. Three of the seven Crys exhibited a high toxicity to Asian corn borer over the controls. One of them, Cry1Ab-Gc, a chimeric Cry1Ab being replaced with the domain-III of Cry1Gc, showed the highest toxicity to rice stem borer when it was over-expressed in Oryza sativa. Furthermore, it was also transformed into maize, backcrossed into commercial maize inbred lines and then produced hybrid to evaluate their commercial value. Transgenic maize performed significant resistance to the Asian corn borer without affecting the yield. We further showed that this new protein did not have adverse effects on the environment. Our results indicated that domain III swapped of Crys could be used as an efficient method for developing new engineered insecticidal protein.
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http://dx.doi.org/10.1038/s41598-022-16426-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307649PMC
July 2022

Mesenchymal/stromal stem cells: necessary factors in tumour progression.

Cell Death Discov 2022 Jul 22;8(1):333. Epub 2022 Jul 22.

Department of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China.

Mesenchymal/stromal stem cells (MSCs) are a crucial component of the tumour microenvironment (TME). They can be recruited from normal tissues into the TME and educated by tumour cells to transform into tumour-associated MSCs, which are oncogenic cells that promote tumour development and progression by impacting or transforming into various kinds of cells, such as immune cells and endothelial cells. Targeting MSCs in the TME is a novel strategy to prevent malignant processes. Exosomes, as communicators, carry various RNAs and proteins and thus link MSCs and the TME, which provides options for improving outcomes and developing targeted treatment.
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http://dx.doi.org/10.1038/s41420-022-01107-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307857PMC
July 2022

Tumor-derived exosomes in the cancer immune microenvironment and cancer immunotherapy.

Cancer Lett 2022 Jul 11:215823. Epub 2022 Jul 11.

Department of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China; Shenyang Clinical Medical Research Center for Diagnosis, Treatment and Health Management of Early Digestive Cancer, Shenyang, 110032, China. Electronic address:

Tumor-derived exosomes (TDEs) are key immune regulators in the tumor microenvironment. They have been shown to reshape the immune microenvironment and prevent antitumor immune responses via their immunosuppressive cargo, thereby determining responsiveness to cancer therapy. By delivering suppressive cargo to the immune cells, TDEs directly or indirectly influence the functions and antitumor activities of immune cells. TDE-based therapy is emerging as a cutting-edge and promising strategy for inhibiting tumor progression or enhancing antitumor immunity. Therefore, in this study, we reviewed the mechanism by which TDEs regulate immune cells and their applications in immunotherapy.
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http://dx.doi.org/10.1016/j.canlet.2022.215823DOI Listing
July 2022

Analysis of the subcellular location of lncRNA SLC16A1-AS1 and its interaction with premature miR-5088-5p in oral squamous cell carcinoma.

Odontology 2022 Jul 12. Epub 2022 Jul 12.

Department of Geriatrics, Daqing Oilfiled General Hospital, No. 9, Sartu District, Daqing, 163000, Heilongjiang, People's Republic of China.

SLC16A1-AS1 is a newly identified lncRNA with different roles in different cancers. MiR-5088-5p is an oncogenic miRNA in breast cancer. However, their participation in oral squamous cell carcinoma (OSCC) is unknown. We predicted the interaction between SLC16A1-AS1 and miR-5088-5p, and this study was carried out to explore the crosstalk between them in OSCC. A total of 56 OSCC patients donated OSCC and paired non-tumor tissues, which were used to detect the differential expression of SLC16A1-AS1 and miR-5088-5p (mature and premature). Analysis of the subcellular location of SLC16A1-AS1 in OSCC cells and its direct interaction with premature miR-5088-5p was performed with cellular fractionation assay and RNA pull-down assay, respectively. The involvement of SLC16A1-AS1 in miR-5088-5p maturation was studied with overexpression assay. BrdU assay was performed to detect cell proliferation after transfection. OSCC tissue samples exhibited decreased expression levels of SLC16A1-AS1 and premature miR-5088-5p, but increased the expression levels of mature miR-5088-5p. SLC16A1-AS1 was detected in both nucleus and cytoplasm samples of OSCC cells and its direct interaction with premature miR-5088-5p was confirmed. Overexpression of SLC16A1-AS1 in OSCC cells resulted in inhibited maturation of miR-5088-5p. SLC16A1-AS1 suppressed the enhancing effects of miR-5088-5p on cell proliferation. SLC16A1-AS1 was downregulated in OSCC and it may inhibit cell proliferation by suppressing maturation of miR-5088-5p.
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http://dx.doi.org/10.1007/s10266-022-00712-wDOI Listing
July 2022

Augmented reality calibration using feature triangulation iteration-based registration for surgical navigation.

Comput Biol Med 2022 Jul 6;148:105826. Epub 2022 Jul 6.

Beijing Engineering Research Center of Mixed Reality and Advanced Display, School of Optics and Photonics, Beijing Institute of Technology, Beijing, 100081, China.

Background: Marker-based augmented reality (AR) calibration methods for surgical navigation often require a second computed tomography scan of the patient, and their clinical application is limited due to high manufacturing costs and low accuracy.

Methods: This work introduces a novel type of AR calibration framework that combines a Microsoft HoloLens device with a single camera registration module for surgical navigation. A camera is used to gather multi-view images of a patient for reconstruction in this framework. A shape feature matching-based search method is proposed to adjust the size of the reconstructed model. The double clustering-based 3D point cloud segmentation method and 3D line segment detection method are also proposed to extract the corner points of the image marker. The corner points are the registration data of the image marker. A feature triangulation iteration-based registration method is proposed to quickly and accurately calibrate the pose relationship between the image marker and the patient in the virtual and real space. The patient model after registration is wirelessly transmitted to the HoloLens device to display the AR scene.

Results: The proposed approach was used to conduct accuracy verification experiments on the phantoms and volunteers, which were compared with six advanced AR calibration methods. The proposed method obtained average fusion errors of 0.70 ± 0.16 and 0.91 ± 0.13 mm in phantom and volunteer experiments, respectively. The fusion accuracy of the proposed method is the highest among all comparison methods. A volunteer liver puncture clinical simulation experiment was also conducted to show the clinical feasibility.

Conclusions: Our experiments proved the effectiveness of the proposed AR calibration method, and revealed a considerable potential for improving surgical performance.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105826DOI Listing
July 2022

Quercitrin protects human bronchial epithelial cells from oxidative damage.

Open Med (Wars) 2022 22;17(1):375-383. Epub 2022 Feb 22.

Department of Traditional Chinese Medicine, Wuhan No. 1 Hospital, Wuhan 43022, Hubei, China.

Chronic obstructive pulmonary disease (COPD) is mainly caused by cigarette smoking (CS), with oxidative stress being one key component during its pathogenesis. This study aimed to investigate the effects of quercitrin (QE) on cigarette smoke extract (CSE)-induced cell apoptosis and oxidative stress in human bronchial epithelial cells (HBECs) and its underlying mechanism. HBECs were treated with 2% CSE for 24 h to establish COPD cellular models. CCK-8 assay and flow cytometry analysis were performed to evaluate cell viability and apoptosis, respectively. Western blotting was applied to examine protein levels and ELISA kits were used to examine contents of the indicated oxidant/antioxidant markers. The results demonstrated that CSE promoted apoptosis and suppressed viability of HBECs and QE reversed these effects. CSE caused increase in T-AOC, superoxide dismutase, and glutathione (GSH) peroxidase contents and decrease in MDA, reactive oxygen species , and GSH contents in HBECs, which were rescued by QE treatment. The CSE-induced Nrf2 nuclear translocation and elevation of NAD(P)H: quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) expression were also reversed by QE in HBECs. The mitogen-activated protein kinase (MAPK) signaling was activated by CSE and further suppressed by QE in HBECs. Collectively, QE exerts a protective role in HBECs against cell apoptosis and oxidative damage via inactivation of the Nrf2/HO-1/NQO1 pathway and the MAPK/ERK pathway.
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http://dx.doi.org/10.1515/med-2022-0416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864058PMC
February 2022

Economic Policy Uncertainty and Family Firm Innovation: Evidence From Listed Companies in China.

Front Psychol 2022 14;13:901051. Epub 2022 Jun 14.

School of Business Administration, Northeastern University, Shenyang, China.

With the advancement of China's economic transformation, the impact of economic policy uncertainty on family firms has become increasingly significant. The "familism" of family firms makes them more motivated to maintain family harmony, pursue innovative activities, and the long-term development of enterprises when faced with economic policy uncertainty. In this paper, we employed the data of listed Chinese family firms from 2010 to 2018 to analyze the impact of economic policy uncertainty on family business innovation activities, analyze the inherent characteristics of family firm innovation, and find the path that enables the innovative activities of family firms and provides a valuable experience for the innovation of private enterprises in economic policy uncertainty. We provide evidence that economic policy uncertainty positively relates to family firm innovation. Moreover, the relationship is affected by factors such as directors' executive background and access to state-owned equity. Further analysis indicates that economic policy uncertainty can promote family firms' innovation activities by improving their risk-taking, internal capital market circulation, and reducing political connections.
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http://dx.doi.org/10.3389/fpsyg.2022.901051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237622PMC
June 2022

Integrative mRNA and Long Noncoding RNA Analysis Reveals the Regulatory Network of Floral Bud Induction in Longan ( Lour.).

Front Plant Sci 2022 14;13:923183. Epub 2022 Jun 14.

Insititute of Genetics and Breeding in Horticultural Plants, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China.

Longan ( Lour.) is a tropical/subtropical fruit tree of significant economic importance. Floral induction is an essential process for longan flowering and plays decisive effects on the longan yield. Due to the instability of flowering, it is necessary to understand the molecular mechanisms of floral induction in longan. In this study, mRNA and long noncoding RNA (lncRNA) transcriptome sequencing were performed using the apical buds of fruiting branches as materials. A total of 7,221 differential expressions of mRNAs (DEmRNAs) and 3,238 differential expressions of lncRNAs (DElncRNAs) were identified, respectively. KEGG enrichment analysis of DEmRNAs highlighted the importance of starch and sucrose metabolic, circadian rhythms, and plant hormone signal transduction pathways during floral induction. Combining the analysis of weighted gene co-expression network (WGCNA) and expression pattern of DEmRNAs in the three pathways, specific transcriptional characteristics at each stage during floral induction and regulatory network involving co-expressed genes were investigated. The results showed that sucrose metabolism and auxin signal transduction may be crucial for the growth and maturity of autumn shoots in September and October (B1-B2 stage); starch and sucrose metabolic, circadian rhythms, and plant hormone signal transduction pathways participated in the regulation of floral bud physiological differentiation together in November and December (B3-B4 stage) and the crosstalk among three pathways was also found. Hub genes in the co-expression network and key DEmRNAs in three pathways were identified. The circadian rhythm genes and were found to activate gene through the photoperiod core factor genes, and they were co-expressed with auxin, gibberellin, abscisic acid, ethylene signaling genes, and sucrose biosynthesis genes at B4 stage. A total of 12 hub-DElncRNAs had potential for positively affecting their distant target genes in three putative key pathways, predominantly in a -transcriptional manner. A hypothetical model of regulatory pathways and key genes and lncRNAs during floral bud induction in longan was proposed finally. Our studies will provide valuable clues and information to help elucidate the potential molecular mechanisms of floral initiation in longan and woody fruit trees.
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http://dx.doi.org/10.3389/fpls.2022.923183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237614PMC
June 2022

Early pregnancy human decidua gamma/delta T cells exhibit tissue resident and specific functional characteristics.

Mol Hum Reprod 2022 Jul;28(8)

Center of Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

A successful pregnancy is a complicated process that builds upon two aspects of the maternal immune system that need to be balanced. As one of the indispensable groups of immune cell at the maternal-fetal interface, the decidual gamma/delta (γδ) T cells have attracted research attention in normal pregnancy and miscarriage. However, the role of γδ T cells in fetal growth remains poorly understood. Here, we found that the γδ T-cell population resident in decidua during early pregnancy was enriched and secreted growth factors including growth differentiation factor 15 and bone morphogenetic protein 1. A diminution in such growth factors may impair fetal development and result in fetal growth restriction. We also observed that early decidual γδ T cells exhibited stronger cytokine-secretion characteristics, but that their cytotoxic actions against A549 cells were weaker, compared with γδ T cells in peripheral blood mononuclear cells (PBMCs). In addition, the functional abilities of early decidual γδ T cells in promoting trophoblast cell proliferation, migration, invasion and tube formation were also significantly more robust than in γδ T cells of PBMCs. These findings highlight the importance of γδ T cells in fetal growth and maternal immunotolerance during pregnancy and show that they differ from γδ T cells in PBMCs. We thus recommend additional investigation in this research area to further elucidate a role for γδ T cells in pregnancy.
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http://dx.doi.org/10.1093/molehr/gaac023DOI Listing
July 2022

School Psychological Environment and Learning Burnout in Medical Students: Mediating Roles of School Identity and Collective Self-Esteem.

Front Psychol 2022 2;13:851912. Epub 2022 Jun 2.

School of Biomedical Engineering, Anhui Medical University, Hefei, China.

Learning burnout is an important indicator that reflects an individual's learning state. Understanding the influencing factors and mechanism of learning burnout of medical students has practical significance for improving their mental health. This study aimed to explore the mediating roles of school identity and collective self-esteem between school psychological environment and learning burnout in medical students. A total of 2,031 medical students (942 men and 1,089 women, age range: 17-23 years) were surveyed using the School Psychological Environment Questionnaire (SPEQ), School Identity Questionnaire (SIQ), Collective Self-esteem Scale (CSES), and Learning Burnout Scale (LBS). The results showed the following: (1) school psychological environment had a negative effect on learning burnout among medical students (β = -0.19, < 0.001), and (2) school identity and collective self-esteem played significant mediating roles between school psychological environment and learning burnout [95% CI = (-0.43, -0.31)]. Specifically, there were three paths that school psychological environment and learning burnout: first, through the independent mediating role of school identity; second, through the independent mediating role of collective self-esteem; and third, through the chain mediating roles of school identity and collective self-esteem. The findings reveal that school psychological environment not only directly influences the learning burnout of medical students but also indirectly influences it through school identity and collective self-esteem. Thus, this study has some important implications for prevention and intervention of learning burnout among medical students.
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http://dx.doi.org/10.3389/fpsyg.2022.851912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200954PMC
June 2022

[Systematic review and Meta-analysis of efficacy and safety of Binghuang Fule Ointment in treatment of eczema].

Zhongguo Zhong Yao Za Zhi 2022 May;47(10):2802-2810

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China.

To expeditiously assess the efficacy and safety of Binghuang Fule Ointment in the treatment of eczema, we screened out the papers with randomized controlled trials(RCTs) for studying the efficacy of Binghuang Fule Ointment in the treatment of eczema from CNKI, VIP, Wanfang, SinoMed, PubMed, Web of Science, Cochrane Library, and EMbase and then performed Meta-analysis of the included studies via RevMan 5.4. A total of 19 studies were included, involving 1 919 cases(973 cases in the experimental group and 946 cases in the control group). Meta-analysis results showed that Binghuang Fule Ointment combined with conventional western medicine had better efficacy score index(clinical effectiveness ≥60%)(RR=1.32, 95%CI[1.13, 1.55], P=0.000 4) and lower recurrence rate(RR=0.37, 95%CI[0.20, 0.65], P=0.000 7) than conventional western medicine alone. The adverse reactions(RR=1.05, 95%CI[0.52, 2.15], P=0.88) did not show significant difference between the two groups. The application of Binghuang Fule Ointment alone had better efficacy score index(clinical effectiveness≥60%)(RR=1.20, 95%CI[1.09, 1.33], P=0.000 3) than conventional western medicine alone and the adverse reactions(RR=0.92, 95%CI[0.45, 1.89], P=0.82) insignificantly different from conventional western medicine alone. Binghuang Fule Ointment alone or combined with conventional western medicine demonstrated better effective in remission of symptoms and signs(clinical effectiveness)(RR=1.41, 95%CI[1.07, 1.85], P=0.01) than conventional western medicine alone. Compared with the single application of western medicine, Binghuang Fule Ointment alone or combined with conventional western medicine has better curative effect, low recurrence rate, and equivalent safety in the treatment of eczema. Nevertheless, owing to the low quality of the included papers, randomized controlled trials with large sample size, multiple centers, high methodological quality are needed to further verify the efficacy and safety of Binghuang Fule Ointment in the treatment of eczema.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220215.501DOI Listing
May 2022

Effects of sodium-glucose cotransporter-2 inhibitors and aldosterone antagonists, in addition to renin-angiotensin system antagonists, on major adverse kidney outcomes in patients with type 2 diabetes and chronic kidney disease: A systematic review and network meta-analysis.

Diabetes Obes Metab 2022 Jun 16. Epub 2022 Jun 16.

Department of Endocrinology and Metabolism, The People's Hospital of China Medical University, The People's Hospital of Liaoning Province, Shenyang, P. R. China.

Aims: To compare the efficacy of sodium-glucose cotransporter-2 (SGLT2) inhibitors, nonsteroidal mineralocorticoid receptor antagonists (MRAs), selective aldosterone antagonists and nonselective aldosterone antagonists, on top of renin-angiotensin-aldosterone system (RAAS) blockade, in reducing kidney-specific composite events, cardiovascular outcomes, and other events of special interest in participants with type 2 diabetes (T2D) and chronic kidney disease (CKD).

Methods: PubMed, EMBASE and CENTRAL were searched for studies published up to January 20, 2022. Randomized clinical trials enrolling participants with T2D and CKD were included, in which SGLT2 inhibitors, nonsteroidal MRAs, selective aldosterone antagonists and nonselective aldosterone antagonists were compared with either each other, or with placebo or no treatment. A network meta-analysis using a Bayesian approach was performed. The primary outcome was a kidney-specific composite event. Secondary outcomes included death from cardiovascular causes, nonfatal myocardial infarction, nonfatal stroke, hospitalization for heart failure, and all-cause mortality. We also examined blood pressure and safety outcomes of interest, including acute kidney injury, hyperkalaemia, hyponatraemia, and volume reduction events. All research was conducted according to a protocol registered in the PROSPERO database (CRD42022307113).

Results: This meta-analysis of 17 trials randomizing 22 981 participants found SGLT2 inhibitors (odds ratio [OR] 0.62, 95% confidence interval [CI] 0.52 to 0.73) and nonsteroidal MRAs (OR 0.76, 95% CI 0.66 to 0.88) were associated with significantly lower kidney-specific composite events than the control groups. Nonsteroidal MRAs (OR 0.78, 95% CI 0.66 to 0.92) and SGLT2 inhibitors (OR 0.57, 95% CI 0.45 to 0.72) were associated with greater reductions in hospitalization for heart failure than the control groups. SGLT2 inhibitors were associated with a lower risk of hospitalization for heart failure events compared with nonsteroidal MRAs (OR 0.73, 95% CI 0.55-0.97). SGLT2 inhibitors were associated with a reduction in cardiovascular death (OR 0.80, 95% CI 0.65 to 0.98) and all-cause mortality (OR 0.79, 95% CI 0.66 to 0.93) compared with the control groups. When compared to the control groups, both nonsteroidal MRAs (weighted mean difference [WMD] -10.96, 95% CI -20.49 to -1.46) and SGLT2 inhibitors (WMD -3.50, 95% CI -6.01 to -1.013) were linked with lower systolic blood pressure, nonsteroidal MRAs (OR 2.27, 95% CI 2.02 to 2.56) and nonselective aldosterone antagonists (OR 3.22, 95% CI 1.43 to 7.66) were associated with an increased risk of hyperkalaemia, nonsteroidal MRAs were linked with an increased risk of hyponatraemia (OR 16.56, 95% CI 2.78 to 455.19), and SGLT2 inhibitors were associated with an increased risk of volume reduction events (OR 1.28, 95% CI 1.06 to 1.56). SGLT2 inhibitors were ranked the best for our primary and secondary outcomes. Confidence in the evidence was often high or moderate.

Conclusions: In this network meta-analysis, the use of SGLT2 inhibitors or nonsteroidal MRAs, combined with RAAS blockade, was associated with a reduction in kidney-specific composite events and hospitalization for heart failure events in patients with T2D and CKD compared to placebo or no treatment. SGLT2 inhibitors were associated with a lower risk of hospitalization for heart failure events compared with nonsteroidal MRAs. Use of SGLT2 inhibitors was associated with lower mortality than placebo or no treatment.
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http://dx.doi.org/10.1111/dom.14801DOI Listing
June 2022

Novel STAT3 Inhibitors Targeting STAT3 Dimerization by Binding to the STAT3 SH2 Domain.

Front Pharmacol 2022 27;13:836724. Epub 2022 May 27.

Centre for Cancer Biomarkers (CCBIO), Department of Clinical Science, University of Bergen, Bergen, Norway.

Our drug discovery model has identified two novel STAT3 SH2 domain inhibitors 323-1 and 323-2 (delavatine A stereoisomers) in a series of experiments. computational modeling, drug affinity responsive target stability (DARTS), and fluorescence polarization (FP) assays altogether determined that 323-1 and 323-2 directly target the STAT3 SH2 domain and inhibited both phosphorylated and non-phosphorylated STAT3 dimerization. Computational docking predicted that compound 323s bind to three subpockets of the STAT3 SH2 domain. The 323s inhibition of STAT3 dimerization was more potent than the commercial STAT3 SH2 domain inhibitor S3I-201 in the co-immunoprecipitation assay, correlating with computational docking data. The fluorescence polarization assay further confirmed that the compound 323s target the STAT3 SH2 domain by competitively abrogating the interaction between STAT3 and the SH2-binding peptide GpYLPQTV. Compared with S3I-201, the 323 compounds exhibited stronger inhibition of STAT3 and reduced the level of IL-6-stimulated phosphorylation of STAT3 (Tyr705) in LNCaP cells over the phosphorylation of STAT1 (Tyr701) induced by IFN-ɣ in PC3 cells or the phosphorylation of STAT1 (Ser727) in DU145 cells. Both compounds downregulated STAT3 target genes MCL1 and cyclin D1. Thus, the two compounds are promising lead compounds for the treatment of cancers with hyper-activated STAT3.
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http://dx.doi.org/10.3389/fphar.2022.836724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196127PMC
May 2022

Study on the Effect of Salt Stress on Yield and Grain Quality Among Different Rice Varieties.

Front Plant Sci 2022 31;13:918460. Epub 2022 May 31.

Yibang Agriculture Technology Development Co., Ltd., Dongying, China.

Salt is one of the main factors limiting the use of mudflats. In this study, the yield, quality, and mineral content of rice seeds under salt stress were investigated. A pot experiment was conducted with Yangyugeng2, Xudao9, and Huageng5 under 0, 17.1, 25.6, and 34.2 mM NaCl of salt concentration treatments. The results showed that salt stress can significantly decrease panicle number, grain number per panicle, 1000-grain weight and yield of rice, and the panicle number was among other things the main cause of yield loss under saline conditions. When the salt concentration is less than 34.2 mM NaCl, the salt stress increases the brown rice rate and milled rice rate, thus significant increasing head milled rice rate of salt-sensitive varieties but decreasing in salt-tolerant varieties. In addition, the grain length is more sensitive than grain width to salt stress. This study also indicates that different varieties of rice exhibit different salt tolerance under salt stress, the three rice varieties in this study, in order of salt tolerance, are Xudao9, Huageng5, and Yangyugeng2. Salt stress will increase the appearance, viscosity, degree of balance, and taste value, and decrease the hardness of rice when salt concentration is less than 17.1 mM NaCl in Yangyugeng2 and Huageng5 or 25.6 mM NaCl in Xudao9. The differences in starch pasting properties among rice varieties in this study are larger than those caused by salt stress. The uptake capacity of K, Mg, P, S, and Cu ions in the seeds of different rice varieties significantly vary, and salt stress causes significant differences in the uptake capacity of K, Na, and Cu ions in rice seeds. Rice varieties with high salt tolerance can be selected for the development and utilization of mudflats, and low concentration of salt stress will increase the rice quality, all of which are meaningful to agricultural production.
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http://dx.doi.org/10.3389/fpls.2022.918460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194819PMC
May 2022

Infrared Spectroscopy of Stepwise Hydration Motifs of Sulfur Dioxide.

J Phys Chem Lett 2022 Jun 16;13(24):5654-5659. Epub 2022 Jun 16.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Experimental characterization of microscopic events and behaviors of SO-HO interactions is crucial to understanding SO atmospheric chemistry but has been proven to be very challenging due to the difficulty in size selection. Here, size-dependent development of SO hydrate structure and cluster growth in the SO(HO) ( = 1-16) complexes was probed by infrared spectroscopy based on threshold photoionization using a tunable vacuum ultraviolet free electron laser. Spectral changes with cluster size demonstrate that the sandwich structure initially formed at = 1 develops into cycle structures with the sulfur and oxygen atoms in a two-dimensional plane ( = 2 and 3) and then into three-dimensional cage structures ( ≥ 4). SO is favorably bound to the surface of larger water clusters. These stepwise features of SO hydration on various sized water clusters contribute to understanding the reactive sites and electrophilicity of SO on cloud droplets, which may have important atmospheric implications for studying the SO-containing aerosol systems.
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http://dx.doi.org/10.1021/acs.jpclett.2c01472DOI Listing
June 2022

Ice nucleation imaged with X-ray spectro-microscopy.

Environ Sci Atmos 2022 May 7;2(3):335-351. Epub 2022 Feb 7.

Swiss Light Source, Paul Scherrer Institute 5232 Villigen PSI Switzerland.

Ice nucleation is one of the most uncertain microphysical processes, as it occurs in various ways and on many types of particles. To overcome this challenge, we present a heterogeneous ice nucleation study on deposition ice nucleation and immersion freezing in a novel cryogenic X-ray experiment with the capability to spectroscopically probe individual ice nucleating and non-ice nucleating particles. Mineral dust type particles composed of either ferrihydrite or feldspar were used and mixed with organic matter of either citric acid or xanthan gum. We observed ice nucleation using scanning transmission X-ray microscopy (STXM) and identified unique organic carbon functionalities and iron oxidation state using near-edge X-ray absorption fine structure (NEXAFS) spectroscopy in the new environmental ice cell, termed the ice nucleation X-ray cell (INXCell). Deposition ice nucleation of ferrihydrite occurred at a relative humidity with respect to ice, , between ∼120-138% and temperatures, ∼ 232 K. However, we also observed water uptake on ferrihydrite at the same when deposition ice nucleation did not occur. Although, immersion freezing of ferrihydrite both in pure water droplets and in aqueous citric acid occurred at or slightly below conditions for homogeneous freezing, the effect of ferrihydrite particles acting as a heterogeneous ice nucleus for immersion freezing was small. Microcline K-rich feldspar mixed with xanthan gum was also used in INXCell experiments. Deposition ice nucleation occurred at conditions when xanthan gum was expected to be highly viscous (glassy). At less viscous conditions, immersion freezing was observed. We extended a model for heterogeneous and homogeneous ice nucleation, named the stochastic freezing model (SFM). It was used to quantify heterogeneous ice nucleation rate coefficients, mimic the competition between homogeneous ice nucleation; water uptake; deposition ice nucleation and immersion freezing, and predict the and at which ice was observed. The importance of ferrihydrite to act as a heterogeneous ice nucleating particle in the atmosphere using the SFM is discussed.
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http://dx.doi.org/10.1039/d1ea00077bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119033PMC
May 2022

Epigenetics-mediated pathological alternations and their potential in antiphospholipid syndrome diagnosis and therapy.

Autoimmun Rev 2022 Aug 9;21(8):103130. Epub 2022 Jun 9.

Department of Laboratory Medicine, Peking University Third Hospital, Beijing, 100191, China; Core Unit of National Clinical Research Center for Laboratory Medicine, Peking University Third Hospital, Beijing 100191, China. Electronic address:

APS (antiphospholipid syndrome) is a systematic autoimmune disease accompanied with venous or arterial thrombosis and poor pregnant manifestations, partly attributing to the successive elevated aPL (antiphospholipid antibodies) and provoked prothrombotic and proinflammatory molecules production. Nowadays, most researches focus on the laboratory detection and clinic features of APS, but its precise etiology remains to be deeply explored. As we all know, the dysfunction of ECs (endothelial cells), monocytes, platelets, trophoblasts and neutrophils are key contributors to APS progression. Especially, their epigenetic variations, mainly including the promoter CpGs methylation, histone PTMs (post-translational modifications) and ncRNAs (noncoding RNAs), result in genes expression or silence engaged in inflammation initiation, thrombosis formation, autoimmune activation and APOs (adverse pregnancy outcomes) in APS. Given the potential of epigenetic markers serving as diagnostic biomarkers or therapeutic targets of APS, and the encouraging advancements in epigenetic drugs are being made. In this review, we would systematically introduce the epigenetic underlying mechanisms for APS progression, comprehensively elucidate the functional mechanisms of epigenetics in boosting ECs, monocytes, platelets, trophoblasts and neutrophils. Lastly, the application of epigenetic alterations for probing novel diagnostic, specific therapeutic and prognostic strategies would be proposed.
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http://dx.doi.org/10.1016/j.autrev.2022.103130DOI Listing
August 2022

Rapid and sustained restoration of astrocytic functions by ketamine in depression model mice.

Biochem Biophys Res Commun 2022 08 14;616:89-94. Epub 2022 Apr 14.

State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen, China. Electronic address:

Molecules with fast-acting antidepressant effects have potentials to become new antidepressants. Here we report the fast-acting (1hr) antidepressant effects of ketamine (10 mg/kg, i.p.) in chronic adreno-cortico-tropic-hormone (ACTH)-induced and chronic unpredictable mild stress (CUMS)-induced depression mouse models. These behavioral anti-depressant effects are associated with normalized expression of glutamate transporter-1(GLT-1), glial fibrillary acidic protein (GFAP), brain-derived neurotrophic factor (BDNF) and eukaryotic elongation factor 2 phosphorylation (p-eEF2) in the prelimbic prefrontal cortex (PrL-PFC). Excitatory neurons in PrL also showed reduced ambient glutamate responses to synaptic stimulation, and reduced ambient NMDA receptor responses after ketamine injection. Interestingly, ketamine induced biochemical and electrophysiological changes still occurred with GLT-1 knockdown in PrL, suggesting that elevated GLT-1 level is not required for ketamine to exert its antidepressant effect. At the same time, ketamine did not elevate GLT-1 level in the isolated astrocytes, suggesting distinct contributions from neurons and astrocytes to ketamine-induced changes.
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http://dx.doi.org/10.1016/j.bbrc.2022.03.068DOI Listing
August 2022

Interaction of fluoride exposure and CREB1 gene polymorphisms on thyroid function in school-age children.

Chemosphere 2022 Sep 28;303(Pt 2):135156. Epub 2022 May 28.

Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address:

Objectives: To evaluate the effects of CREB1 gene polymorphisms and long-term exposure to fluoride on thyroid function of children.

Study Design: A total of 424 children (including 226 boys and 198 girls) aged 7-12 years old were enrolled in Kaifeng, China by cross-sectional study in 2017. The concentrations of urinary fluoride (UF) and creatinine (UCr) were measured using fluoride ion-selective electrode assay and creatinine assay kit (picric acid method), respectively. The concentration of UCr-adjusted UF (CUF) was calculated. Children were divided into high fluoride exposure group (HFG, CUF >1.41 mg/L) and low fluoride exposure group (LFG, CUF ≤1.41 mg/L) according to the median concentration of CUF (1.41 mg/L). The serum thyroid-stimulating hormone (TSH), total triiodothyronine (TT3) and total thyronine (TT4) levels were detected by the radiation immunoassay. The B-mode ultrasound was performed to test the thyroid volume (Tvol). Genotyping of CREB1 gene was conducted by a custom-by-design 48-plex SNPscan™ Kit. Associations between CUF concentration, CREB1 single nucleotide polymorphisms (SNPs) and thyroid function were assessed by multiple linear regression models.

Results: Negative and positive associations between serum TT4 level (β = -0.721, 95%CI: -1.209, -0.234) and Tvol (β = 0.031, 95%CI: 0.011, 0.050) and CUF concentration were observed respectively. Children carrying CREB1 rs11904814 TG and rs2254137 AC genotypes had lower TT3 levels (P < 0.05). Children in HFG carrying rs11904814 TT, rs2253206 GG genotypes and rs6740584 C allele easily manifested lower serum TT4 levels (P < 0.05). Moreover, interactions between excessive fluoride exposure and CREB1 SNPs on Tvol were observed, and the interaction among different loci of CREB1 gene could modify serum TT3 level (P < 0.05, respectively).

Conclusions: Fluoride could alter children's serum TT4 levels and Tvol. Interactions between fluoride exposure and CREB1 polymorphisms may modify thyroid volume of children.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135156DOI Listing
September 2022

Anticardiolipin IgA as a Potential Risk Factor for Pregnancy Morbidity in Patients with Antiphospholipid Syndrome.

Lab Med 2022 May 29. Epub 2022 May 29.

Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China.

Background: Antiphospholipid syndrome (APS) is an autoimmune disorder that is characterized by venous or arterial thrombosis and/or obstetric morbidity in the constant presence of persistent antiphospholipid antibodies (aPLs). In patients with APS, the relationship between production of immunoglobulin (Ig)A antiphospholipid antibodies and adverse events in pregnancy is still unclear. As a result of massive trials, the clinical efficiency of IgA-aPLs is used to evaluate pregnancy outcomes in patients with APS.

Methods: We enrolled 381 female patients with APS and 93 healthy pregnant women. Silica clotting time ratio, dilute Russell viper venom time (dRVVT) ratio, and 6 aPLs, including IgA/IgG/IgM isotypes aβ2GPI and IgA/IgG/IgM isotypes anticardiolipin (aCL), were detected using commercial kits.

Results: We found no significant differences in laboratory parameters between patients with APS and the control group. The total prevalence of aCL IgA was 2.9%; the prevalence of aβ2GPI IgA was 3.4%. Only 1.3% of the individuals who tested aCL-positive (5/381) had isolated aCL IgA. Similarly, isolated aβ2GPI IgA was present in only 0.8% (3/381) of the aβ2GPI-positive subjects. Meanwhile, aCL IgA showed the maximum area under the curve (AUC) of 0.666 (95% CI, 0.60-0.73; P < .001), followed by dRVVT ratio (AUC = 0.649; 0.58-0.72; P < .001).

Conclusion: Positive aCL IgA and aβ2GPI IgA ratios were extremely low for each isolated isotype of aPLs. For patients with APS who experienced fetal loss, aCL IgA may be utilized as a risk factor for pregnancy loss among patients with APS. Establishing a standardized diagnosis of IgA aPLs is also important for these patients.
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http://dx.doi.org/10.1093/labmed/lmac028DOI Listing
May 2022

Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure.

Environ Res 2022 Sep 21;212(Pt D):113530. Epub 2022 May 21.

State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, PR China; School of Civil Engineering, Lanzhou University of Technology, Lanzhou, 730070, PR China. Electronic address:

Distribution of intracellular (iARGs) and extracellular ARGs (eARGs) in manure anaerobic digestion (AD) process coupled with two types of biochar (BC and BP) were investigated. And the effects of biochar on the conjugation transfer of ARGs were explored by deciphering the interaction of biochar with bacterial stress responses, physiological metabolism and antibiotic resistances. Results showed that AD process could effectively remove all the detected eARGs with efficiency of 47.4-98.2%. The modified biochar (BP) with larger specific surface area (SSA) was propitious to decrease the absolute copy number of extracellular resistance genes. AD process could effectively remove iARGs by inhibiting the growth of host bacteria. The results of structural equation models (SEM) indicated that biochar put indirect influences on the fate of ARGs (λ = -0.23, P > 0.05). Analysis on oxidative stress levels, antioxidant capacity, DNA damage-induced response (SOS) response and energy generation process demonstrated that biochar induced the oxidative stress response of microorganisms and enhanced the antioxidant capacity of bacteria. The elevated antioxidant capacity negatively affected SOS response, amplified cell membrane damage and further weakened the energy generation process, resulted in the inhibition of horizontal transfer of ARGs.
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http://dx.doi.org/10.1016/j.envres.2022.113530DOI Listing
September 2022

An Altered Microbiota in the Lower and Upper Female Reproductive Tract of Women with Recurrent Spontaneous Abortion.

Microbiol Spectr 2022 06 23;10(3):e0046222. Epub 2022 May 23.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospitalgrid.411642.4, Beijing, China.

Recurrent spontaneous abortion (RSA) is a complex multifactorial disease. Recently, the microbiota of the female reproductive tract, as an emerging factor in RSA, has gradually attracted the attention of many clinical researchers. Here, we reported that the microbiota of the lower and upper female reproductive tracts from patients with RSA showed no significant differences in alpha diversity compared to that of controls. Beta diversity was significantly higher in the RSA group than in the control group in the vaginal microbiota ( = 0.036), cervical microbiota ( = 0.010) and microbiota from uterine lavage fluid ( = 0.001). In addition, dramatic decreases in gamma interferon and interleukin-6 cytokine levels were observed in the RSA group. In conclusion, our data suggested altered microbial biodiversity in the vagina, cervix and uterine lavage fluid in the RSA group. Alterations in the microbiota in the uterine cavity could be associated with altered cytokine levels, which might be a risk factor for RSA pathogenesis. Moreover, the microbiota composition differed markedly from the lower genital tract to the uterine cavity, and the microbiota in the uterine cavity also distinctly varied between endometrial tissue and uterine lavage fluid in the RSA group. Hence, sampling with these two methods simultaneously allowed a more comprehensive perspective of microbial colonization in the uterine cavity. As an obstacle to pregnancy, recurrent spontaneous abortion (RSA) can be caused by a variety of factors, and a current understanding of the etiology of RSA is still lacking; half of cases have an unknown cause. A substantial fraction of patients show no improvement after treatment. Since the microbiota of the female reproductive tract has been proposed as an emerging factor in RSA patients, further investigation is needed to provide guidance for clinical therapy. In general, this is the first report describing the distinct alterations of the vaginal, cervical, and uterine microbiota in RSA, not just that in the vagina. Furthermore, another major strength of this study derived from the further in-depth investigation and analysis of the characteristics of the microbiota colonizing the upper female genital tract in RSA, which provided a more comprehensive view for investigating the uterine microbiota.
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http://dx.doi.org/10.1128/spectrum.00462-22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241707PMC
June 2022

Development and critical evaluation of a novel fluorescent nanosensor based on a molecularly imprinted polymer for the rapid detection of procymidone in ginseng.

Analyst 2022 Jun 13;147(12):2718-2730. Epub 2022 Jun 13.

Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, School of Life Science, Jilin University, Changchun, People's Republic of China.

Effective methods are required to quantify the organochlorine pesticide procymidone due to its potentially harmful effects toward human health and the environment. Here, hydrophilic hollow imprinted microspheres were prepared a simple method as fluorescent sensors (@MIH-prm) for the sensitive and selective detection of PRM in ginseng. A new method of adsorption efficiency evaluation for @MIH-prm was subsequently introduced (EBS%), the effective binding site, which provided a comprehensive evaluation of the performance compared with conventional methods. The results showed that @MIH-prm could detect PRM in filtered and diluted ginseng juice with high sensitivity (LOD, 0.569 nM) and a rapid detection rate (quantitative detection of PRM within 18 min). Good selectivity was observed in the presence of combinations of different pesticides, and the adsorption of PRM could be described by the pseudo-second-order kinetic model. PRM concentrations exhibited good linearity over 1-40 nM, and the accuracy (recovery rates, 99.2 to 103.1%) and precision (RSD at 1.0 × 10 M, 3.14%) indicated that @MIH-prm could be used for the quantitative analysis of PRM in complex matrices. Hence, @MIH-prm has good application potential in pollution control monitoring and enforcement.
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http://dx.doi.org/10.1039/d1an02186aDOI Listing
June 2022

A pathogenic mechanism associated with myopathies and structural birth defects involves TPM2-directed myogenesis.

JCI Insight 2022 06 22;7(12). Epub 2022 Jun 22.

Department of Developmental Biology, Washington University in St. Louis, St. Louis, Missouri, USA.

Nemaline myopathy (NM) is the most common congenital myopathy, characterized by extreme weakness of the respiratory, limb, and facial muscles. Pathogenic variants in Tropomyosin 2 (TPM2), which encodes a skeletal muscle-specific actin binding protein essential for sarcomere function, cause a spectrum of musculoskeletal disorders that include NM as well as cap myopathy, congenital fiber type disproportion, and distal arthrogryposis (DA). The in vivo pathomechanisms underlying TPM2-related disorders are unknown, so we expressed a series of dominant, pathogenic TPM2 variants in Drosophila embryos and found 4 variants significantly affected muscle development and muscle function. Transient overexpression of the 4 variants also disrupted the morphogenesis of mouse myotubes in vitro and negatively affected zebrafish muscle development in vivo. We used transient overexpression assays in zebrafish to characterize 2 potentially novel TPM2 variants and 1 recurring variant that we identified in patients with DA (V129A, E139K, A155T, respectively) and found these variants caused musculoskeletal defects similar to those of known pathogenic variants. The consistency of musculoskeletal phenotypes in our assays correlated with the severity of clinical phenotypes observed in our patients with DA, suggesting disrupted myogenesis is a potentially novel pathomechanism of TPM2 disorders and that our myogenic assays can predict the clinical severity of TPM2 variants.
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http://dx.doi.org/10.1172/jci.insight.152466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309062PMC
June 2022

A Synthetic Protocell-Based Heparin Scavenger.

Small 2022 May 15:e2201790. Epub 2022 May 15.

Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, Espoo, 02150, Finland.

Heparin is a commonly applied blood anticoagulant agent in clinical use. After treatment, excess heparin needs to be removed to circumvent side effects and recover the blood-clotting cascade. Most existing heparin antidotes rely on direct heparin binding and complexation, yet selective compartmentalization and sequestration of heparin would be beneficial for safety and efficiency. However, such systems have remained elusive. Herein, a semipermeable protein-based microcompartment (proteinosome) is loaded with a highly positively charged chitosan derivative, which can induce electrostatics-driven internalization of anionic guest molecules inside the compartment. Chitosan-loaded proteinosomes are subsequently employed to capture heparin, and an excellent heparin-scavenging performance is demonstrated under physiologically relevant conditions. Both the highly positive scavenger and the polyelectrolyte complex are confined and shielded by the protein compartment in a time-dependent manner. Moreover, selective heparin-scavenging behavior over serum albumin is realized through adjusting the localized scavenger or surrounding salt concentrations at application-relevant circumstances. In vitro studies reveal that the cytotoxicity of the cationic scavenger and the produced polyelectrolyte complex is reduced by protocell shielding. Therefore, the proteinosome-based systems may present a novel polyelectrolyte-scavenging method for biomedical applications.
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http://dx.doi.org/10.1002/smll.202201790DOI Listing
May 2022

Analytical and clinical performance evaluation of a new high-sensitivity cardiac troponin I assay.

Clin Chem Lab Med 2022 07 16;60(8):1299-1307. Epub 2022 May 16.

Department of Laboratory Medicine, Peking University Third Hospital, Beijing, P.R. China.

Objectives: To validate the analytical performance and diagnostic accuracy for non-ST-segment elevation myocardial infarction (NSTEMI) with a new high-sensitivity cardiac troponin I (hs-cTnI) assay on the automated light-initiated chemiluminescent assay (LiCA) platform.

Methods: Comprehensive analytical validations were performed, and the 99th percentile upper reference limit (URL) from apparently healthy individuals were established. We evaluated the diagnostic performance of the assay for NSTEMI.

Results: The limit of quantitation (LoQ) were 1.9 ng/L (20% CV) and 5.1 ng/L (10% CV). The sex-specific 99th percentile URLs were 17.6 ng/L (4.2% CV) for men (age 20-79y) and 14.2 ng/L (4.9% CV) for women (age 19-89y) in serum, 14.4 ng/L (4.9% CV) for men (age 19-88y) and 12.9 ng/L (5.2% CV) for women (age 19-87y) in plasma, respectively. Detection rates in healthy individuals were from 98.7 to 99.1%. The correlation coefficient and median bias between LiCA and Architect were 0.985 and 0.1% (-2.0-2.9%) in full analytical range of serum specimens. In lower range (<100 ng/L), LiCA had an overall positive bias 6.7% (-1.6-13.3%), R=0.949. At the specific medical decision levels (15.2, 26.2 and 64.0 ng/L), assay difference was estimated to be <10%. No significant differences on AUC, sensitivity and specificity, NPV and PPV were found between LiCA and Architect for the diagnosis of NSTEMI.

Conclusions: LiCA hs-cTnI is a precise, highly sensitive and specific assay that meets the requirement of a 3rd generation (level 4) high-sensitivity method. The diagnostic accuracy of LiCA assay for NSTEMI is comparable to the established Architect hs-cTnI assay.
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http://dx.doi.org/10.1515/cclm-2021-1136DOI Listing
July 2022
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