Publications by authors named "Shuo Yang"

593 Publications

A nonsynonymous polymorphism (rs117179004, T392M) of hyaluronidase 1 (HYAL1) is associated with increased risk of idiopathic pulmonary fibrosis in Southern Han Chinese.

J Clin Lab Anal 2021 May 4:e23782. Epub 2021 May 4.

Department of Internal Medicine and Genetic Diagnosis Center, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, China.

Background: Idiopathic pulmonary fibrosis (IPF) is a genetic heterogeneous disease with high mortality and poor prognosis. Hyaluronidase 1 (HYAL1) was found to be upregulated in fibroblasts from IPF patients, and overexpression of HYAL1 could prevent human fetal lung fibroblast proliferation. However, the genetic correlation between the HYAL1 and IPF or connective tissue diseases related interstitial lung disease (CTD-ILD) has not been determined.

Methods: A two-stage study was conducted in Southern Han Chinese population. We sequenced the coding regions and flanking regulatory regions of HYAL1 in stage one (253 IPF cases and 125 controls). A statistically significant variant was further genotyped in stage two (162 IPF cases, 182 CTD-ILD cases, and 225 controls).

Results: We identified a nonsynonymous polymorphism (rs117179004, T392M) significantly associated with increased IPF risk (dominant model: OR = 2.239, 95% CI = 1.212-4.137, p = 0.010 in stage one; OR = 2.383, 95% CI = 1.376-4.128, p = 0.002 in stage two). However, we did not observe this association in CTD-ILD (OR = 1.401, 95% CI = 0.790-2.485, p = 0.248).

Conclusion: Our findings suggest that the nonsynonymous polymorphism (rs117179004, T392M) may confer susceptibility to IPF in Southern Han Chinese, but is not associated with susceptibility to CTD-ILD.
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http://dx.doi.org/10.1002/jcla.23782DOI Listing
May 2021

Boosting Multilabel Semantic Segmentation for Somata and Vessels in Mouse Brain.

Front Neurosci 2021 12;15:610122. Epub 2021 Apr 12.

Engineering Research Center of Hubei Province for Clothing Information, School of Mathematics and Computer Science, Wuhan Textile University, Wuhan, China.

Deep convolutional neural networks (DCNNs) are widely utilized for the semantic segmentation of dense nerve tissues from light and electron microscopy (EM) image data; the goal of this technique is to achieve efficient and accurate three-dimensional reconstruction of the vasculature and neural networks in the brain. The success of these tasks heavily depends on the amount, and especially the quality, of the human-annotated labels fed into DCNNs. However, it is often difficult to acquire the gold standard of human-annotated labels for dense nerve tissues; human annotations inevitably contain discrepancies or even errors, which substantially impact the performance of DCNNs. Thus, a novel boosting framework consisting of a DCNN for multilabel semantic segmentation with a customized Dice-logarithmic loss function, a fusion module combining the annotated labels and the corresponding predictions from the DCNN, and a boosting algorithm to sequentially update the sample weights during network training iterations was proposed to systematically improve the quality of the annotated labels; this framework eventually resulted in improved segmentation task performance. The microoptical sectioning tomography (MOST) dataset was then employed to assess the effectiveness of the proposed framework. The result indicated that the framework, even trained with a dataset including some poor-quality human-annotated labels, achieved state-of-the-art performance in the segmentation of somata and vessels in the mouse brain. Thus, the proposed technique of artificial intelligence could advance neuroscience research.
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http://dx.doi.org/10.3389/fnins.2021.610122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071950PMC
April 2021

High discrepancy in thrombotic events in non-small cell lung cancer patients with different genomic alterations.

Transl Lung Cancer Res 2021 Mar;10(3):1512-1524

Department of Medical Oncology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Background: Acute complications, such as venous thromboembolism (VTE), are common in patients with advanced severe lung cancers. However, current VTE risk scores cannot adequately identify high-risk patients with non-small cell lung cancer (NSCLC). The study proposed to elucidated the incidence of thromboembolism (TE) in patients with different oncogenic aberrations and the impact of these aberrations on the efficacy of targeted therapy in patients with NSCLC.

Methods: A systemic review was conducted in Web of Science, PubMed, Embase and the Cochrane Library to evaluate the incidence of TE in different molecular subtypes of NSCLC. Data from patients diagnosed of advanced NSCLC who harboring anaplastic lymphoma kinase (ALK) or ROS proto-oncogene 1 receptor tyrosine kinase (ROS1) rearrangements since 2016 to 2019 were also retrospectively collected. A meta-analysis with random-effects model, sensitivity analysis and publication bias were performed. The principal summary measure was incidence of thrombotic events in NSCLC patients. And the efficacy of tyrosine kinase inhibitor (TKI) therapy was compared between the two subgroups.

Results: A total of 5,767 cases from 20 studies were included in the analysis of the incidence of thrombosis in patients with different oncogenic alterations. The pooled analysis showed a higher risk of thrombosis in ROS1-fusion types (41%, 95% CI: 35-47%) and ALK-fusion types (30%, 95% CI: 24-37%) than in EGFR-mutation (12%, 95% CI: 8-17%), KRAS-mutation (25%, 95% CI: 13-50%), and wild-type (14%, 95% CI: 10-20%) cases. A high prevalence of thrombosis (ALK: 24.4%; ROS1: 32.6%) was observed in the Shanghai Pulmonary Hospital (SPH) cohort of 224 patients with ALK or ROS1 fusion. Furthermore, patients with embolism had significantly shorter progression-free survival (PFS) after TKI therapy than those without embolism, both in the ALK+ cohort (5.6 12.9 months, P<0.0001) and in the ROS1+ cohort (9.6 17.6 months, P=0.0481).

Conclusions: NSCLC patients with ALK/ROS1 rearrangements are more likely to develop thrombosis than patients with other oncogenic alterations. Thrombosis may also be associated with an inferior response and PFS after TKI therapy.
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http://dx.doi.org/10.21037/tlcr-20-1290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044490PMC
March 2021

Controlled Growth of Large-Sized and Phase-Selectivity 2D GaTe Crystals.

Small 2021 Apr 19:e2007909. Epub 2021 Apr 19.

School of Materials Science and Engineering, Nanyang Technological University (NTU), Singapore, 639798.

GaTe has recently attracted significant interest due to its direct bandgap and unique phase structure, which makes it a good candidate for optoelectronics. However, the controllable growth of large-sized monolayer and few-layer GaTe with tunable phase structures remains a great challenge. Here the controlled growth of large-sized GaTe with high quality, chemical uniformity, and good reproducibility is achieved through liquid-metal-assisted chemical vapor deposition method. By using liquid Ga, the rapid growth of 2D GaTe flakes with high phase-selectivity can be obtained due to its reduced reaction temperature. In addition, the method is used to synthesize many Ga-based 2D materials and their alloys, showing good universality. Raman spectra suggest that the as-grown GaTe own a relatively weak van der Waals interaction, where monoclinic GaTe displays highly-anisotropic optical properties. Furthermore, a p-n junction photodetector is fabricated using GaTe as a p-type semiconductor and 2D MoSe as a typical n-type semiconductor. The GaTe/MoSe heterostructure photodetector exhibits large photoresponsivity of 671.52 A W and high photo-detectivity of 1.48 × 10 Jones under illumination, owing to the enhanced light absorption and good quality of as-grown GaTe. These results indicate that 2D GaTe is a promising candidate for electronic and photoelectronic devices.
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http://dx.doi.org/10.1002/smll.202007909DOI Listing
April 2021

Disruption of Circadian Transcriptome in Lung by Acute Sleep Deprivation.

Front Genet 2021 30;12:664334. Epub 2021 Mar 30.

Center for Brain Science, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Inadequate sleep prevails in modern society and it impairs the circadian transcriptome. However, to what extent acute sleep deprivation (SD) has impact on the circadian rhythms of peripheral tissues is not clear. Here, we show that in mouse lung, a 10-h acute sleep deprivation can alter the circadian expression of approximately 3,000 genes. We found that circadian rhythm disappears in genes related to metabolism and signaling pathways regulating protein phosphorylation after acute sleep deprivation, while the core circadian regulators do not change much in rhythmicity. Importantly, the strong positive correlation between mean expression and amplitude (E-A correlation) of cycling genes has been validated in both control and sleep deprivation conditions, supporting the energetic cost optimization model of circadian gene expression. Thus, we reveal that acute sleep deprivation leads to a profound change in the circadian gene transcription that influences the biological functions in lung.
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http://dx.doi.org/10.3389/fgene.2021.664334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042274PMC
March 2021

No causal effects of plasma homocysteine levels on the risk of coronary heart disease or acute myocardial infarction: A Mendelian randomization study.

Eur J Prev Cardiol 2021 04 16;28(2):227-234. Epub 2019 Dec 16.

Clinical Laboratory of The Affiliated Cancer Hospital, Guangxi Medical University, China.

Background: Although many observational studies have shown an association between plasma homocysteine levels and cardiovascular diseases, controversy remains. In this study, we estimated the role of increased plasma homocysteine levels on the etiology of coronary heart disease and acute myocardial infarction.

Methods: A two-sample Mendelian randomization study on disease was conducted, i.e. "coronary heart disease" (n = 184,305) and "acute myocardial infarction" (n = 181,875). Nine single nucleotide polymorphisms, which were genome-wide significantly associated with plasma homocysteine levels in 57,644 subjects from the Coronary ARtery DIsease Genome wide Replication and Meta-analysis (CARDIoGRAM) plus The Coronary Artery Disease (C4D) Genetics (CARDIoGRAMplusC4D) consortium genome-wide association study and were known to be associated at p < 5×10-8, were used as an instrumental variable.

Results: None of the nine single nucleotide polymorphisms were associated with coronary heart disease or acute myocardial infarction (p > 0.05 for all). Mendelian randomization analysis revealed no causal effects of plasma homocysteine levels, either on coronary heart disease (inverse variance weighted; odds ratio = 1.015, 95% confidence interval = 0.923-1.106, p = 0.752) or on acute myocardial infarction (inverse variance weighted; odds ratio = 1.037, 95% confidence interval = 0.932-1.142, p = 0.499). The results were consistent in sensitivity analyses using the weighted median and Mendelian randomization-Egger methods, and no directional pleiotropy (p = 0.213 for coronary heart disease and p = 0.343 for acute myocardial infarction) was observed. Sensitivity analyses confirmed that plasma homocysteine levels were not significantly associated with coronary heart disease or acute myocardial infarction.

Conclusions: The findings from this Mendelian randomization study indicate no causal relationship between plasma homocysteine levels and coronary heart disease or acute myocardial infarction. Conflicting findings from observational studies might have resulted from residual confounding or reverse causation.
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http://dx.doi.org/10.1177/2047487319894679DOI Listing
April 2021

An in situ microtomography apparatus with a laboratory x-ray source for elevated temperatures of up to 1000 °C.

Rev Sci Instrum 2021 Mar;92(3):033704

College of Engineering, Peking University, Beijing 100871, People's Republic of China.

An elevated-temperature in situ microtomography apparatus that can measure internal damage parameters under tensile loads at high temperatures up to 1000 °C is developed using a laboratory x-ray source. The maximum resolution of the apparatus can reach 3 µm by a reasonable design. A high-temperature environment is accomplished by means of a heating chamber based on a radiation technique using four halogen lamps with ellipsoidal reflectors. To obtain high resolution, the chamber is much smaller in the direction of the x-ray beam than in the other two directions. Two thin aluminum windows are chosen as the chamber walls perpendicular to and intersecting the x-ray beam. A material testing machine equipped with two synchronous rotating motors is specially designed for mechanical loading and 360° rotation of the specimen, and customized grips are developed to conduct tensile tests. A microfocus x-ray source and a high-resolution detector are used to produce and detect X rays, and the distances among the x-ray source, specimen, and high-resolution detector can be adjusted to obtain different resolutions. To show the main functions and usability of the apparatus, carbon-fiber-reinforced silicon-carbide matrix specimens are subjected to in situ x-ray microtomography tensile tests at 800 °C and 1000 °C, and the crack propagation behavior under thermomechanical coupling loads is studied.
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http://dx.doi.org/10.1063/5.0038026DOI Listing
March 2021

Down-regulation of miR-340-5p promoted osteogenic differentiation through regulation of runt-related transcription factor-2 (RUNX2) in MC3T3-E1 cells.

Bioengineered 2021 Dec;12(1):1126-1137

Department of Endocrinology, The People's Hospital of China Medical University, The People's Hospital of Liaoning Province, Shenyang, Liaoning P.R. China.

Diabetic osteoporosis (DOP) is a chronic complication of diabetes in the skeletal system. High level of miR-340-5p may be harmful to the bone formation. In this study, the DOP model of rats was successfully established via streptozotocin (STZ) and ovariectomy (OVX) treatment. It was manifested by reduced body weight, insulin level, alkaline phosphatase (ALP) activity, and osteocalcin (OCN) and collagen-I expressions, as well as increased concentration of fasting blood glucose. Moreover, we found that miR-340-5p expression was increased while runt-related transcription factor-2 (RUNX2) was decreased in femurs. Furthermore, the effects of miR-340-5p on osteogenic differentiation (OD) in high glucose (HG)-treated MC3T3-E1 cells were explored. Exposure to OD and HG contributed to elevated miR-340-5p level. Inhibition of miR-340-5p enhanced ALP level, calcium deposition, and OCN, collagen-I and RUNX2 levels. On the contrary, miR-340-5p overexpression reversed these promotional effects. Luciferase assay indicated that RUNX2 may be a target gene of miR-340-5p. Moreover, RUNX2 deficiency decreased miR-340-5p inhibition-induced ALP activity, calcium accumulation and OCN, collagen-I, RUNX2 levels. In short, the above findings revealed that inhibition of miR-340-5p facilitated osteogenic differentiation through regulating RUNX2 in MC3TC-E1 cells, which provided targeted therapeutic strategies for the treatment of DOP.
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http://dx.doi.org/10.1080/21655979.2021.1905259DOI Listing
December 2021

Stronger secondary pollution processes despite decrease in gaseous precursors: A comparative analysis of summer 2020 and 2019 in Beijing.

Environ Pollut 2021 Jun 10;279:116923. Epub 2021 Mar 10.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex, Beijing Key Laboratory of Indoor Air Quality Evaluation and Control, Tsinghua University, Beijing, 100084, China.

To control the spread of COVID-19, China implemented a series of lockdowns, limiting various offline interactions. This provided an opportunity to study the response of air quality to emissions control. By comparing the characteristics of pollution in the summers of 2019 and 2020, we found a significant decrease in gaseous pollutants in 2020. However, particle pollution in the summer of 2020 was more severe; PM levels increased from 35.8 to 44.7 μg m, and PM increased from 51.4 to 69.0 μg m from 2019 to 2020. The higher PM was caused by two sandstorm events on May 11 and June 3, 2020, while the higher PM was the result of enhanced secondary formation processes indicated by the higher sulfate oxidation rate (SOR) and nitrate oxidation rate (NOR) in 2020. Higher SOR and NOR were attributed mainly to higher relative humidity and stronger oxidizing capacity. Analysis of PM distribution showed that severe haze occurred when particles within Bin2 (size ranging 1-2.5 μm) dominated. SO and SO remained stable under different periods at 0.5 and 0.8, respectively, indicating that SO existed mainly in smaller particles. Decreases in NO and increases in NO from clean to polluted conditions, similar to the variations in PM distribution, suggest that NO played a role in the worsening of pollution. O concentrations were higher in 2020 (108.6 μg m) than in 2019 (96.8 μg m). Marked decreases in fresh NO alleviated the titration of O. Furthermore, the oxidation reaction of NO that produces NO was dominant over the photochemical reaction of NO that produces O, making NO less important for O pollution. In comparison, a lower VOC/NO ratio (less than 10) meant that Beijing is a VOC-limited area; this indicates that in order to alleviate O pollution in Beijing, emissions of VOCs should be controlled.
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http://dx.doi.org/10.1016/j.envpol.2021.116923DOI Listing
June 2021

Exon 20 YVMA insertion is associated with high incidence of brain metastasis and inferior outcome of chemotherapy in advanced non-small cell lung cancer patients with HER2 kinase domain mutations.

Transl Lung Cancer Res 2021 Feb;10(2):753-765

Department of Medical Oncology, Shanghai Pulmonary Hospital and Thoracic Cancer Institute, Tongji University School of Medicine, Shanghai, China.

Background: Chemotherapy remains the standard care for mutated advanced non-small cell lung cancer (NSCLC) even though several targeted drugs showed promising results in preliminary stages. This study aimed to investigate the association of mutation variants with clinical features and the efficacy of chemotherapy in patients with mutated advanced NSCLC.

Methods: ARMS-PCR was used to identify HER2 mutation in patients without common oncogenic alterations. Patients with detailed information were further enrolled for analysis of clinical features and efficacy of first line chemotherapy. Survival data was analyzed by Kaplan-Meier method and compared by log-rank test. Brain metastasis incidence was analyzed and compared by Gray's test.

Results: YVMA insertion accounted for the majority (68.4%, 67/98) of HER2 mutation, and associated with significantly higher incidence of baseline extrathoracic metastasis (P=0.009), notably brain metastasis (P=0.004). Among 82 patients those received first line chemotherapy, YVMA insertion remarkably associated with inferior treatment outcomes, namely, a significantly shorter median progression free survival (PFS) and lower objective response rate (ORR) both in total patients (PFS: 5.2 7.7 m, P=0.038; ORR: 30.9% 51.9%, P=0.09) and pemetrexed subgroup (PFS: 5.2 6.5 m, P=0.022; ORR: 31.8% 60.0%, P=0.054). Multivariate analysis further established YVMA insertion as prognostic factor of worse PFS both for total patients (HR =1.578, 95% CI, 0.956-2.606) and patients received pemetrexed-based chemotherapy (HR =1.789, 95% CI, 1.013-3.160). In addition, YVMA insertion associated with higher incidence of lifetime brain metastasis (P=0.002) compared by Gray's test, with estimated 12-month brain metastasis incidence as 40.2% compared with 3.6% in the non-YVMA group.

Conclusions: YVMA insertion is associated with a higher incidence of brain metastasis, and inferior outcomes to chemotherapy than non-YVMA variants in patients with advanced NSCLC and HER2 kinase domain mutations, which emphasized the unmet need of more potent anti-cancer therapies with high blood-brain barrier (BBB) penetration capacity for patients with YVMA insertion.
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http://dx.doi.org/10.21037/tlcr-20-559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947396PMC
February 2021

AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitis.

J Exp Med 2021 May;218(5)

Department of Immunology, Key Laboratory of Immunological Environment and Disease, State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing, China.

The role of the PYHIN family member absent in melanoma 2 (AIM2), another important inflammasome sensor, in EAE remains unclear. In this study, we found that AIM2 negatively regulates the pathogenesis of EAE independent of inflammasome activation. AIM2 deficiency enhanced microglia activation and infiltration of peripheral immune cells into the CNS, thereby promoting neuroinflammation and demyelination during EAE. Mechanistically, AIM2 negatively regulates the DNA-PK-AKT3 in microglia to control neuroinflammation synergistically induced by cGAS and DNA-PK. Administration of a DNA-PK inhibitor reduced the severity of the EAE. Collectively, these findings identify a new role for AIM2 in controlling the onset of EAE. Furthermore, delineation of the underlying inflammasome-independent mechanism highlights cGAS and DNA-PK signaling as potential targets for the treatment of heterogeneous MS.
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http://dx.doi.org/10.1084/jem.20201796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961553PMC
May 2021

Metabolomic Signature Between Metabolically Healthy Overweight/Obese and Metabolically Unhealthy Overweight/Obese: A Systematic Review.

Diabetes Metab Syndr Obes 2021 4;14:991-1010. Epub 2021 Mar 4.

Department of Endocrinology and Metabolism, The First Hospital of Jilin University, Changchun, 130021, People's Republic of China.

The clinical manifestations of overweight/obesity are heterogeneous and complex. In contrast to metabolically unhealthy overweight/obese (MUO), a particular sub-group of obese patients who are considered as metabolically healthy overweight/obese (MHO), display favorable metabolic profiles characterized by high levels of insulin sensitivity, normal blood pressure, as well as favorable lipid, inflammation, hormone, liver enzyme, and immune profiles. While only a few available studies focused on the metabolic files underlying the obese phenotypes, the current review aimed to perform a systematic review of available studies focusing on describing the metabolomic signature between MUO and MHO. We did the systematic search for literature on MEDLINE (PubMed), the Cochrane Library, EMBASE, and searched for the references of relevant manuscripts from inception to 29 May 2020. After critical selection, 20 studies were eligible for this systematic review and evaluated by using QUADOMICS for quality assessment. Eventually, 12 of 20 studies were classified as "high quality". Branched-chain amino acids (isoleucine, leucine, and valine), aromatic amino acids (phenylalanine and tyrosine), lipids (palmitic acid, palmitoleic acid, oleic acid, eicosapentaenoic acid, and docosahexaenoic acid), and acylcarnitines (propionyl carnitine) levels might be elevated in MUO. The current results suggested that MHO showed a favorable trend in the overall metabolic signature. More longitudinal studies are needed to elaborate deeply on the metabolic pathway and the relationship between metabolic patterns and the occurrence of the disease.
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http://dx.doi.org/10.2147/DMSO.S294894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939496PMC
March 2021

Chemical Fueling Enables Molecular Complexification of Self-Replicators*.

Angew Chem Int Ed Engl 2021 05 8;60(20):11344-11349. Epub 2021 Apr 8.

Centre for Systems Chemistry, Stratingh Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

Unravelling how the complexity of living systems can (have) emerge(d) from simple chemical reactions is one of the grand challenges in contemporary science. Evolving systems of self-replicating molecules may hold the key to this question. Here we show that, when a system of replicators is subjected to a regime where replication competes with replicator destruction, simple and fast replicators can give way to more complex and slower ones. The structurally more complex replicator was found to be functionally more proficient in the catalysis of a model reaction. These results show that chemical fueling can maintain systems of replicators out of equilibrium, populating more complex replicators that are otherwise not readily accessible. Such complexification represents an important requirement for achieving open-ended evolution as it should allow improved and ultimately also new functions to emerge.
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http://dx.doi.org/10.1002/anie.202016196DOI Listing
May 2021

Curcumin protects BEAS‑2B cells from PM‑induced oxidative stress and inflammation by activating NRF2/antioxidant response element pathways.

Int J Mol Med 2021 04 8;47(4). Epub 2021 Feb 8.

Department of Pulmonary and Critical Care Medicine, Wuhan No. 1 Hospital, Wuhan, Hubei 430000, P.R. China.

Fine particulate matter (PM) with an average aerodynamic diameter of <2.5 µm can cause severe lung injury. Oxidative stress and inflammation are considered the main outcomes of PM exposure. Curcumin is a well‑known antioxidant; however, its effect on PM‑induced oxidative injury in airway epithelial cells remains unclear. In the present study, it was demonstrated that pre‑treatment with curcumin significantly reduced the PM‑induced apoptosis of BEAS‑2B human bronchial epithelial cells by decreasing the level of intercellular reactive oxygen species. Western blot analysis revealed that curcumin increased the expression of nuclear factor erythroid 2‑related factor 2 (NRF2) and regulated the transcription of downstream genes, particularly those encoding antioxidant enzymes. Moreover, curcumin reduced the PM‑induced expression and production of inflammatory factors, and induced the expression of the anti‑inflammatory factors, interleukin (IL)‑5 and IL‑13. Taken together, the present study demonstrates that curcumin protects BEAS‑2B cells against PM‑induced oxidative damage and inflammation, and prevents cell apoptosis by increasing the activation of NRF2‑related pathways. It is thus suggested that curcumin may be a potential compound for use in the prevention of PM‑induced tissue injury.
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http://dx.doi.org/10.3892/ijmm.2021.4878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895536PMC
April 2021

Oxygen doping in antimony sulfide nanosheets to facilitate catalytic conversion of polysulfides for lithium-sulfur batteries.

Chem Commun (Camb) 2021 Apr 1;57(26):3255-3258. Epub 2021 Mar 1.

Key Laboratory of Carbon Materials of Zhejiang Province, Wenzhou University, Wenzhou 325035, P. R. China.

A high-performance catalyst, O-doped SbS nanosheets (SS-O NSs), is synthesized and introduced into lithium-sulfur batteries. Owing to their good conductivity, strong adsorbability/catalytic effect to polysulfides and fast Li diffusion, the SS-O NSs-modified cathodes can effectively mitigate the shuttle effect, thus achieving outstanding electrochemical performance.
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http://dx.doi.org/10.1039/d0cc08377aDOI Listing
April 2021

[Effect of Gancao Fuzi Decoction on osteoarthritis and proteomics of articular cartilage in rats].

Zhongguo Zhong Yao Za Zhi 2021 Feb;46(3):661-669

School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.

The rat osteoarthritis model was replicated by injection of sodium iodoacetate into the knee joint cavity, and the effects of Gancao Fuzi Decoction on rat osteoarthritis and the proteome of articular cartilage were investigated. Sixty SD rats weighing 230-250 g were randomly divided into normal group, model group, glucosamine sulfate group, and Gancao Fuzi Decoction high, medium and low dose groups. Osteoarthritis model was induced by intra-articular injection of sodium iodoacetate(3 mg on each leg) in all groups except the normal group. After modeling, each administration group was given intragastric administration for 1 month. During the administration period, joint pain test and joint width measurement were performed every week to observe the autonomous behavior of rats. Enzyme linked immunosorbent assay(ELISA) method was used to detect the contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), matrix metalloproteinase-3(MMP-3), and matrix metalloproteinase tissue inhibitor(TIMP-1) in rat joint lavage fluid. Hematoxylin-eosin(HE) staining was used to observe bone and joint morphology. Nano-LC-LTQ-Orbitrap system was used to detect arti-cular cartilage proteins. The results showed that, compared with the model group, Gancao Fuzi Decoction could significantly improve joint pain and joint swelling in osteoarthritis rats, significantly reduce the contents of TNF-α, IL-1β and MMP-3 in the joint cavity la-vage fluid, increase the content of TIMP-1, and relieve inflammatory diseases such as enlarged joint space, rough cartilage edge, different thickness of cartilage layer, and disordered arrangement of chondrocytes. After comparing the proteins between the groups, 273 differential proteins were screened out. KEGG analysis found that the above differential proteins involved 43 signaling pathways such as systemic lupus erythematosus, among which 11 signaling pathways were related to osteoarthritis. The above results indicated that Gancao Fuzi Decoction had a preventive effect on osteoarthritis, and its mechanism of action may be accomplished by regulating the protein expression of osteoarthritis-related signal pathways.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201210.401DOI Listing
February 2021

Suppression of Calcineurin Enhances the Toxicity of Cry1Ac to .

Front Microbiol 2021 11;12:634619. Epub 2021 Feb 11.

State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

Insect resistance to (Bt) insecticidal proteins has rapidly evolved with the expansion of the planting area of transgenic Bt crops. Pyramiding RNA interference (RNAi) and Bt in crops is urgently needed to counter the rapid increase in pest resistance. The ideal "pyramid" strategy simultaneously targets different action pathways that exert synergetic effects on each other. Here, we identified a dephosphatase, namely, calcineurin (HaCAN), which might enhance the insecticidal activity of Cry1Ac against by regulating immune gene expression via dephosphatase activity, but not by acting as a receptor. Notably, blocking enzyme activity or knocking down endogenous HaCAN significantly promoted the enhancement in Cry1Ac toxicity to insect larvae and cells. Correspondingly, the increase in HaCAN activity reduced the cytotoxicity of Cry1Ac as shown by the heterologous expression of HaCAN. Our results provide a probable that HaCAN is an important candidate gene for pyramiding RNAi and Cry1Ac crops to control cotton bollworm.
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http://dx.doi.org/10.3389/fmicb.2021.634619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904703PMC
February 2021

Vibrational Signature of Dynamic Coupling of a Strong Hydrogen Bond.

J Phys Chem Lett 2021 Mar 26;12(9):2259-2265. Epub 2021 Feb 26.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Elucidating the dynamic couplings of hydrogen bonds remains an important and challenging goal for spectroscopic studies of bulk systems, because their vibrational signatures are masked by the collective effects of the fluctuation of many hydrogen bonds. Here we utilize size-selected infrared spectroscopy based on a tunable vacuum ultraviolet free electron laser to unmask the vibrational signatures for the dynamic couplings in neutral trimethylamine-water and trimethylamine-methanol complexes, as microscopic models with only one single hydrogen bond holding two molecules. Surprisingly broad progression of OH stretching peaks with distinct intensity modulation over ∼700 cm is observed for trimethylamine-water, while the dramatic reduction of this progression in the trimethylamine-methanol spectrum offers direct experimental evidence for the dynamic couplings. State-of-the-art quantum mechanical calculations reveal that such dynamic couplings are originated from strong Fermi resonance between the stretches of hydrogen-bonded OH and several motions of the solvent water/methanol, such as translation, rocking, and bending, which are significant in various solvated complexes commonly found in atmospheric and biological systems.
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http://dx.doi.org/10.1021/acs.jpclett.1c00168DOI Listing
March 2021

ECT2 Increases the stability of EGFR and Tumorigenicity by Inhibiting Grb2 Ubiquitination in Pancreatic Cancer.

Front Oncol 2020 8;10:589241. Epub 2021 Feb 8.

Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

The poor prognosis of patients with pancreatic ductal adenocarcinoma (PDAC) is associated with the invasion and metastasis of tumor cells. Epithelial cell transforming 2 (ECT2) is a guanine nucleotide exchange factor (GEF) of the Rho family of GTPases. It has also been reported that upregulation of ECT2 in pancreatic cancer, but the role and mechanism of ECT2 have not been previously determined. We found that ECT2 was significantly elevated in PDAC tissues and cells, correlated with more advanced AJCC stage, distant metastases, and overall survival of patients with PDAC. Inhibition and overexpression tests showed that ECT2 promoted proliferation, migration and invasion , and promoted tumor growth and metastasis . We determined that ECT2 was involved in the post-translational regulation of Grb2. ECT2 inhibited the degradation of Grb2 through deubiquitination. Furthermore, knockdown of ECT2 downregulated EGFR levels by accelerating EGFR degradation. EGF stimulation facilitated the formation of ECT2-Grb2 complex. Overall, our findings indicated that ECT2 could be used as a promising new therapeutic candidate for PDAC.
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http://dx.doi.org/10.3389/fonc.2020.589241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901901PMC
February 2021

Preferred Film Orientation to Achieve Stable and Efficient Sn-Pb Binary Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Mar 25;13(9):10822-10836. Epub 2021 Feb 25.

Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun 130103, P.R. China.

The preferred orientation of crystalline films in hybrid perovskite materials is known to influence the performance of perovskite solar cells (PSCs). Although the preferred growth along the (112) directions has been reported to promote charge transport within the Pb-based polycrystalline perovskite films, the preferred orientation growth of this facet is still difficult to be achieved due to the higher formation energy compared with the (110) plane. Herein, Sn-Pb binary perovskite films with a well-controlled orientation along the (224) plane were achieved by introducing a simple ultrasonic treatment (UST) into the additive engineering fabricated method. UST is used to process the perovskite precursor solutions of tartaric acid (TA) modified Sn-Pb binary polycrystalline perovskite films to regulate the interactions between PbI/SnI and TA in the intermediate phases. Meanwhile, TA-modulated MACsPbSnI-based perovskite films with a preferred orientation of (224) crystal plane were obtained by precisely controlling the UST time to 15 min. The highest power conversion efficiency (PCE) of 15.59% with less hysteresis and improved stability was achieved, while realizing 8.64 and 25.32% enhancements of PCE compared with that of TA-based and control counterparts with (110) preferred orientation, respectively. Our work provides a promising route to obtain preferred orientation growth of polycrystalline perovskite films. In particular, we have shown that this approach improves the performance of Sn-Pb binary PSCs, while such methodology is quite flexible and could also be applied to other low-/non-toxic PSCs.
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http://dx.doi.org/10.1021/acsami.0c19014DOI Listing
March 2021

Emerging mechanisms and applications of ferroptosis in the treatment of resistant cancers.

Biomed Pharmacother 2020 Oct 17;130:110710. Epub 2020 Sep 17.

Department of General Surgery, the Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, Liaoning, China. Electronic address:

The development of chemotherapy drugs has promoted anticancer treatment, but the effect on tumours is not clear because of treatment resistance; thus, it is necessary to further understand the mechanism of cell death to explore new therapeutic targets. As a new type of programmed cell death, ferroptosis is increasingly being targeted in the treatment of many cancers with clinical drugs and experimental compounds. Ferroptosis is stimulated in tumours with inherently high levels of ferrous ions by a reaction with abundant polyunsaturated fatty acids and the inhibition of antioxidant enzymes, which can overcome treatment resistance in cancers mainly through GPX4. In this review, we focus on the intrinsic cellular regulators against ferroptosis in cancer resistance, such as GPX4, NRF2 and the thioredoxin system. We summarize the application of novel compounds and drugs to circumvent treatment resistance. We also introduce the application of nanoparticles for the treatment of resistant cancers. In conclusion, targeting ferroptosis represents a considerable strategy for resistant cancer treatment.
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http://dx.doi.org/10.1016/j.biopha.2020.110710DOI Listing
October 2020

Hydrogen-substituted graphdiyne/graphene as an sp/sp hybridized carbon interlayer for lithium-sulfur batteries.

Nanoscale 2021 Feb;13(6):3817-3826

Key Laboratory of Carbon Materials of Zhejiang Province, Wenzhou University, Wenzhou 325035, China.

To overcome the shuttle effect in lithium-sulfur (Li-S) batteries, an sp/sp2 hybridized all-carbon interlayer by coating graphene (Gra) and hydrogen-substituted graphdiyne (HsGDY) with a specific surface area as high as 2184 m2 g-1 on a cathode is designed and prepared. The two-dimensional network and rich pore structure of HsGDY can enable the fast physical adsorption of lithium polysulfides (LiPSs). In situ Raman spectroscopy and ex situ X-ray photoelectron spectroscopy (XPS) combined with density functional theory (DFT) computations confirm that the acetylenic bonds in HsGDY can trap the Li+ of LiPSs owing to the strong adsorption of Li+ by acetylenic active sites. The strong physical adsorption and chemical anchoring of LiPSs by the HsGDY materials promote the conversion reaction of LiPSs to further mitigate the shuttling problem. As a result, Li-S batteries integrated with the all-carbon interlayers exhibit excellent cycling stability during long-term cycling with an attenuation rate of 0.089% per cycle at 1 C over 500 cycles.
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http://dx.doi.org/10.1039/d0nr07878fDOI Listing
February 2021

Effects of Artemisia argyi flavonoids on growth performance and immune function in broilers challenged with lipopolysaccharide.

Anim Biosci 2021 Jan 1. Epub 2021 Jan 1.

College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

Objective: This research aimed to study the effects of Artemisia argyi flavonoids (AAF) supplemented in diets on the growth performance and immune function of broiler chickens challenged with lipopolysaccharide (LPS).

Methods: A total of one hundred and ninety-two 1-d-old broiler chicks were assigned into 4 treatment groups, which were, respectively, fed a basal diet(control), fed a diet with 750 mg/kg AAF, fed a basal diet, and challenged with LPS, fed a diet with 750 mg/kg AAF, and challenged with LPS. Each treatment had six pens with 8 chicks per pen. On days 14, 16, 18, 20 (stress phase I) and 28, 30, 32, 34 (stress phase II), broilers were injected with LPS (500 μg/kg BW) or an equivalent amount of saline.

Results: The results demonstrated that dietary AAF significantly improved the body weight (d 21) and alleviated the decrease of average daily gain in broilers challenged with LPS on d 21 and d 35 (p<0.05). Dietary AAF increased bursa fabricius index, and dramatically attenuated the elevation of spleen index caused by LPS on d 35 (p<0.05). Furthermore, serum interleukin-6 (IL-6) concentration decreased with AAF supplementation on d 21 (p<0.05). Diet treatment and LPS challenge exhibited a significant interaction for the concentration of IL-1β (d 21) and IL-6 (d 35) in serum (p<0.05). Additionally, AAF supplementation mitigated the increase of IL-1β, IL-6 in liver and spleen induced by LPS on d 21 and 35 (p<0.05). This study also showed that AAF supplementation significantly reduced the expression of IL-1β (d 21) and NF-κBp65 (d 21 and 35) in liver (p<0.05), and dietary AAF and LPS treatment exhibited significant interaction for the gene expression of IL-6 (d 21), TLR4 (d 35) and MyD88 (d 35) in spleen (p<0.05).

Conclusion: In conclusion, AAF could be used as a potential natural immunomodulator to improve growth performance and alleviate immune stress in broilers challenged with LPS.
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http://dx.doi.org/10.5713/ab.20.0656DOI Listing
January 2021

In-situ surface-enhanced Raman scattering based on MTi nanoflowers: Monitoring and degradation of contaminants.

J Hazard Mater 2021 Jun 22;412:125209. Epub 2021 Jan 22.

School of pharmaceutical sciences, Jilin University, Changchun 130012, China.

Real-time and in-situ monitoring of chemical reactions has attracted great attention in many fields. In this work, we in-situ monitored the photodegradation reaction process of methylene blue (MB) by Surface enhanced Raman scattering (SERS) technique. An effective and versatile SERS platform assembled from MoS nanoflowers (NFs) and TiO nanoparticles (NPs) was prepared successfully. The optimized MoS/TiO substrate (MTi) exhibits not only an ultra-high SERS response but also the excellent catalytic degradation performance to the contaminant MB, which provided a new material for real-time and in-situ monitoring the photodegradation process. Experiments prove that the detection limit is as low as 10 M, and degradation rate is as high as 97.2% in 180 s, respectively. And the activity of the substrate kept in the air for 90 days is almost unchanged. Furthermore, as a practical SERS substrate, MTi can also detect trace amounts of other harmful substances including malachite green (MG), bisphenol A (BPA) and endosulfan. Thus, this study come up with a new orientation at the real-time and in-situ monitoring of photocatalytic reaction and may be applied in environmental monitoring and food security fields in the future.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125209DOI Listing
June 2021

Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes.

J Hematol Oncol 2021 01 29;14(1):21. Epub 2021 Jan 29.

Cancer Centre, Faculty of Health Sciences, University of Macau, Taipa, Macau SPR, China.

Background: B7-H3, an immune-checkpoint molecule and a transmembrane protein, is overexpressed in non-small cell lung cancer (NSCLC), making it an attractive therapeutic target. Here, we aimed to systematically evaluate the value of B7-H3 as a target in NSCLC via T cells expressing B7-H3-specific chimeric antigen receptors (CARs) and bispecific killer cell engager (BiKE)-redirected natural killer (NK) cells.

Methods: We generated B7-H3 CAR and B7-H3/CD16 BiKE derived from an anti-B7-H3 antibody omburtamab that has been shown to preferentially bind tumor tissues and has been safely used in humans in early-phase clinical trials. Antitumor efficacy and induced-immune response of CAR and BiKE were evaluated in vitro and in vivo. The effects of B7-H3 on aerobic glycolysis in NSCLC cells were further investigated.

Results: B7-H3 CAR-T cells effectively inhibited NSCLC tumorigenesis in vitro and in vivo. B7-H3 redirection promoted highly specific T-cell infiltration into tumors. Additionally, NK cell activity could be specially triggered by B7-H3/CD16 BiKE through direct CD16 signaling, resulting in significant increase in NK cell activation and target cell death. BiKE improved antitumor efficacy mediated by NK cells in vitro and in vivo, regardless of the cell surface target antigen density on tumor tissues. Furthermore, we found that anti-B7-H3 blockade might alter tumor glucose metabolism via the reactive oxygen species-mediated pathway.

Conclusions: Together, our results suggest that B7-H3 may serve as a target for NSCLC therapy and support the further development of two therapeutic agents in the preclinical and clinical studies.
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http://dx.doi.org/10.1186/s13045-020-01024-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844995PMC
January 2021

Treatment patterns, clinical outcomes, and healthcare resource use associated with advanced/metastatic lung cancer in China: protocol for a retrospective observational study.

Transl Lung Cancer Res 2020 Dec;9(6):2460-2468

Cancer Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: Lung cancer (LC) is the most common cancer worldwide. The prevalence of LC and rate of associated mortality are high and increasing faster in China than in Western countries. Non-small cell lung cancer (NSCLC) accounts for most LCs. This study aims to be the first large, multi-center, non-interventional retrospective study of treatment patterns (type/duration, number of lines, completion rate), real-world outcomes, and medical costs among Chinese patients with advanced/metastatic NSCLC (IIIb/IV) or extensive-stage small cell LC (ES-SCLC).

Methods: This study will enroll 8,800 patients (≥18 years, with a diagnosis of advanced/metastatic NSCLC made between 1 December 2013 to 30 November 2014) from 35 to 50 Chinese sites. Hospital information systems (HIS) and electronic medical records will be retrospectively reviewed, in adherence with regulatory and ethical requirements. Early-stage treatment (starting from 1 December 2010) of patients with recurrent disease or early disease progression will be examined. Data will be collected at baseline (diagnosis) and 6 and 12 months after this. Observation will end after 3 years or death. Data will be stratified by histology, staging, age, region, health insurance, and epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK) mutation status. Treatment duration and overall survival will be estimated using Kaplan-Meier curves. Descriptive statistics will be used for disease characteristics and patient demographics. Cox-proportional hazards models will be used to examine the impact of demographics/treatment on survival. Treatment patterns and outcome predictors will be explored using multivariate logistic regression.

Discussion: This protocol describes the methodology for collecting real-world data to guide evidence-based clinical practice and inform unmet needs in NSCLC treatment, with potential to identify gaps between guidelines and current practice.

Trial Registration: NCT03505515; data registered on ClinicalTrials.gov: 12h Apr., 2018.
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http://dx.doi.org/10.21037/tlcr-20-1269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815359PMC
December 2020

A composite chitosan derivative nanoparticle to stabilize a W/O/W emulsion: Preparation and characterization.

Carbohydr Polym 2021 Mar 24;256:117533. Epub 2020 Dec 24.

Food Science and Engineering College, Qingdao Agricultural University, No. 700, Changcheng Road, Qingdao 266109, PR China.

For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important. In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied. CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2. CHC-CMC formation was verified by FT-IR when a new peak appeared at 1580 cm; W contained 2 wt % CHC-CMC and W contained 1 wt % sodium alginate, the creaming index (81.6 %) was higher for the emulsions than Tween 80 (67.4 %) after 30 d. Confocal laser scanning microscopy confirmed the double microstructures, in contrast to the collapse with Tween 80, because the CHC-CMC nanoparticles were densely adsorbing on the oil-water interface. This indicates that CHC-CMC has a stronger ability to stabilize W/O/W emulsion than Tween 80.
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http://dx.doi.org/10.1016/j.carbpol.2020.117533DOI Listing
March 2021

Inhibitory effect of PI3Kδ inhibitor idelalisib on proliferation of human myeloid leukemia cells and the reversal effect on drug resistance to adriamycin.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Dec;45(12):1389-1397

Department of Geriatrics/Department of Gastroenterology and Urology Ward II, Hunan Cancer Hospital; Cancer Hospital Affiliated to Xiangya School of Medicine, Central South University, Changsha 410013, China.

Objectives: To investigate the effect of adriamycin (ADM), idelalisib or ADM and their combination on cell proliferation and intracellular concentration of ADM, and to explore the reversal effect of idelalisib on drug resistance to ADM.

Methods: The K562 and K562/ADM cells were respectively treated with ADM and idelalisib at different concentrations. The 50% inhibitory concentration (IC) and drug resistance index (RI) of ADM to the 2 kinds of cells were measured by methyl thiazolyl tetrazolium (MTT) assay. Non-cytotoxic dose (cell inhibition rate <5%) of idelalisib in the 2 kinds of cells was determined. Then the K562 and k562/ADM cells were divided into the following groups: a K562 cells + ADM group, a K562 cells + ADM + idelalisib group, a K562/ADM cells + ADM group, and a K562/ADM cells + ADM + idelalisib group. The survival rates, the intracellular ATP levels, and the relative concentration of intracellular ADM were detected by MTT method, ATP bioluminescence assay (ATP-BLA) and flow cytometry (FCM), respectively.

Results: The cell survival rates were significantly decreased in a dose-dependent manner when the cells were treated with different doses of ADM (0.001-10.000 mg/L ). The IC value of ADM in the K562 and K562/ADM cells were 0.2 mg/L and 1.0 mg/L, respectively. The RI value was 5. The cell survival rates were also significantly decreased in a dose-dependent manner when the cells were treated with different doses of idelalisib (1-50 μmol/L). The non-cytotoxic dose of idelalisib in the K562 and K562/ADM cells were 25 μmol/L and 15 μmol/L, respectively. The cell survival rates in the ADM+ idelalisib group was less than that in the ADM group (<0.05);while there was no statistical difference between the ADM+ idelalisib group and the ADM group in the K562 cells (>0.05). The intracellular ATP level in the K562 cells was about (91.502±0.479) mmol/L, and that in the K562/ADM cells was about (24.311±0.349) mmol/L. The intracellular ATP level in the ADM+ idelalisib group in the K562/ADM cells was less than that in the ADM group (<0.05); but there was no statistical difference between the ADM + idelalisib group and the ADM group in the K562 cells (>0.05). The absorption of intracellular ADM in the ADM + idelalisib group in the K562/ADM cells was more than that in the ADM group (<0.05); but there was no statistical difference in the K562 cells between the 2 groups (>0.05). The exclusion of intracellular ADM in the ADM + idelalisib group in the K562/ADM cells was less than that in the ADM group (<0.05 or <0.01);but there was no statistical difference in the K562 cells between the 2 groups (>0.05).

Conclusions: Idelalisib exerts effect on inhibition of the proliferation in myeloid leukemia K562 and K562/ADM cells, which may partially reverse the drug resistance of K562/ADM cells to ADM. The mechanisms for the effect of idelalisib may be related to increasing the accumulation of ADM and inducing the cell apoptosis in the K562 and K562/ADM cells.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.190728DOI Listing
December 2020

Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration.

ACS Biomater Sci Eng 2020 03 18;6(3):1590-1602. Epub 2020 Feb 18.

Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China.

The treatment of bone defects has plagued clinicians. Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy. In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro. Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites. Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo. The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration. Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01363DOI Listing
March 2020

Impacts of biochar on anaerobic digestion of swine manure: Methanogenesis and antibiotic resistance genes dissemination.

Bioresour Technol 2021 Mar 8;324:124679. Epub 2021 Jan 8.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, PR China; School of Environment, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address:

This work aimed to study the effects of biochar on methanogenesis and antibiotic resistance genes (ARGs) fate during anaerobic digestion (AD) of swine manure (SM) was investigated. Under the optimal biochar dosage of 5-10%, methane yield was significantly improved by 25%, ascribing to the enhancement of direct interspecies electron transfer (DIET). Biochar addition alleviated the need of cytochrome-c as interspecies electron connection components and enriched the microbes involved in DIET. Defluviitoga, Thermovirga and Cloacibacillus were dominant with biochar addition and might participate in DIET together with Methanothrix. The abundance of parC, tetX, bla, bla, aac(6')-Ib-cr, ermB and tetW were significantly reduced in biochar treatments and intI1 removal increased by 15% in B-5% than the control. Structural equation models demonstrated that intI1 (λ = 0.59, P < 0.001) had the most standardized direct effects on ARGs, while biochar indirectly affected ARGs by changing intI1 (λ = 0.41, P < 0.01) and microbial structure (λ = -0.24, P < 0.05).
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http://dx.doi.org/10.1016/j.biortech.2021.124679DOI Listing
March 2021