Publications by authors named "Shuo Wang"

2,000 Publications

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MUC1 regulates AKT signaling pathway by upregulating EGFR expression in ovarian cancer cells.

Pathol Res Pract 2021 Jun 4;224:153509. Epub 2021 Jun 4.

School of Basic Medical Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China. Electronic address:

MUC1, a type I transmembrane glycoprotein, mediates tumor growth and cellular differentiation in various types of cancers. However, the mechanism of MUCI in ovarian cancer has not been fully clarified. In our study, we have observed that MUC1 can play a crucial role in the development and progression of ovarian cancer and act as a predictive marker. We also found that MUC1 could increase the expression of EGFR, and MUC1-EGFR co-administration could promote the cellular growth via the AKT pathway. Taxol is an important drug for treating ovarian cancer, which can prevent cancer recurrence and reduce mortality. Our data have collectively reflected that Taxol can prevent ovarian cancer with abnormal expression of MUC1.
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http://dx.doi.org/10.1016/j.prp.2021.153509DOI Listing
June 2021

Amplified and label-free electrochemical detection of a protease biomarker by integrating proteolysis-triggered transcription.

Biosens Bioelectron 2021 Jun 5;190:113372. Epub 2021 Jun 5.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, PR China.

Cell-free synthetic biology provides a promising strategy for developing high-performance biosensors by integrating with advanced testing technologies. However, the combination of synthetic biology with electrochemical testing techniques is still underdeveloped. Here, we proposed an electrochemical biosensor for the label-free and ultrasensitive detection of target protease biomarker by coupling a protease-responsive RNA polymerase (PR) for signal amplification. Taking tumor biomarker matrix metalloprotease-2 (MMP-2) as a model protease, we employed PR to transduce each proteolysis reaction mediated by MMP-2 into multiple programmable RNA outputs that can be captured by the DNA probes immobilized on a gold electrode. Moreover, the captured RNAs are designed to contain a guanine-rich sequence that can form G-quadruplex and bind to hemin in the presence of potassium ions. In this scenario, the activity of MMP-2 is converted and amplified into the electrochemical signals of hemin. Under the optimal conditions, this PR-based electrochemical biosensor enabled the sensitive detection of MMP-2 in a wide linear dynamic range from 10 fM to 1.0 nM, with a limit of detection of 7.1 fM. Moreover, the proposed biosensor was further applied in evaluating MMP-2 activities in different cell cultures and human tissue samples, demonstrating its potential in the analysis of protease biomarkers in complex clinical samples.
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http://dx.doi.org/10.1016/j.bios.2021.113372DOI Listing
June 2021

The Relationship of Morphological-Hemodynamic Characteristics, Inflammation, and Remodeling of Aneurysm Wall in Unruptured Intracranial Aneurysms.

Transl Stroke Res 2021 Jun 9. Epub 2021 Jun 9.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, China.

Aneurysm wall remodeling (AWR) is an important pathological characteristic in aneurysm wall, which was characterized by abnormal histological structure and inflammation infiltration. In the present study, the aim is to determine the relationships of morphological-hemodynamic characteristics, inflammation, and AWR in intracranial aneurysms (IAs), as well as the pathological basis of morphological-hemodynamic predictors to achieve IA development. For this end, 113 unruptured IAs were prospectively collected from 110 cases. In addition, patient-specific computational fluid dynamics and geometry were adopted to determine hemodynamic and morphological parameters. Moreover, Hematoxylin-Eosin staining was performed to identify the AWR. By performing immunofluorescence, the inflammatory markers were detected. Masson staining was conducted to characterize the characteristics of atherosclerosis in aneurysm wall. To demonstrate the parameters regarding the AWR, a multivariate logistic analysis was conducted. Besides, correlation analyses were conducted to verify the relationship between morphological-hemodynamic and pathological characteristics. For 113 unruptured IAs, no difference was identified in baseline information. AWR was demonstrated in 92 (81.4%) IAs. To be specific, the aneurysm size (odds ratio (OR), 2.63; confidence interval (CI), 1.04-6.67; P = 0.041), size ratio (SR; OR, 1.95; CI, 1.38-2.76; P < 0.001), normalized wall shear stress average (NWSSA; OR, 0.05; CI, 0.01-0.15; P = 0.007), and relative resident time (RRT; OR, 1.28; CI, 1.07-1.53; P = 0.007) were proved as the factors of AWR. As revealed from the results of immunofluorescence, aneurysm size, SR, NWSSA, and RRT were significantly correlated with the level of inflammation in IA tissues. Furthermore, Masson staining revealed that atherosclerosis area in IA tissues and NWSSA was correlated with RRT. In this study, SR, NWSSA, and RRT were demonstrated as the risk factors of AWR. The mentioned parameters could also reflect the characteristics of inflammation and atherosclerosis in aneurysm wall as well. This study revealed that biomechanical stress and inflammation in aneurysm wall are correlated, which might suggest the pathological evidence of morphological-hemodynamic predictors for IA development.
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http://dx.doi.org/10.1007/s12975-021-00917-1DOI Listing
June 2021

Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.

Food Funct 2021 Jun;12(11):4960-4971

College of Basic Medicine, Qingdao University, Qingdao, Shandong, China.

The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS). Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect. In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota. Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation. The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding. Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters. These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
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http://dx.doi.org/10.1039/d0fo03113eDOI Listing
June 2021

Identification of a CD4-binding site-directed antibody with ADCC activity from a chronic HIV-1B'-infected Chinese donor.

Virus Res 2021 Jun 4:198470. Epub 2021 Jun 4.

State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China.; Division of Research of Virology and Immunology, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, China.. Electronic address:

Antibody-dependent cell-mediated cytotoxicity (ADCC) plays an important role in controlling HIV-1 invasion and replication in vivo. Isolation and identification of monoclonal antibodies (mAbs) with ADCC activity help design effective vaccines and develop novel treatment strategies. In this study, we first identified a broad neutralizer who had been infected with an HIV-1B' strain for over 10 years. Next, through probe-specific single-B-cell sorting and PCR amplification, we obtained genes for variable regions of the heavy chain (VHs) and light chain (VLs) of six antibodies and ligated them into an expression vector. After antibody expression and ELISA screening, we obtained a CD4-binding site-directed antibody (451-B4), whose VH and VL originated from the IGHV1-24 and IGLV1-40 germlines, respectively. Although 451-B4 neutralized only the SF162 tier 1 pseudovirus and 398F1 tier 2 pseudovirus, it could mediate comparable ADCC activity to a broadly neutralizing antibody, VRC01. The 451-B4 antibody will be a useful candidate for developing an ADCC-based treatment strategy against HIV-1 replication or latent infection in vivo.
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http://dx.doi.org/10.1016/j.virusres.2021.198470DOI Listing
June 2021

MeCP2 promotes colorectal cancer metastasis by regulating m A methylation via METTL14.

Cancer Sci 2021 Jun 7. Epub 2021 Jun 7.

Department of Pathology, Key Laboratory of Disease Proteomics of Zhejiang Province, Research unit of intelligence classification of tumor pathology and precision therapy Chinese Academy of Medical Sciences (2019RU042), Zhejiang University School of Medicine, Hangzhou, 310058, PR China.

RNA N -methyladenosine (m A) is an emerging regulatory mechanism for tumor progression in several types of cancer. However, the underlying regulation mechanisms of m A methylation in colorectal cancer (CRC) remain unknown. Although the oncogenic function of methyl CpG binding protein 2 (MeCP2) have been reported, it is still unclear whether MeCP2 could alter RNA m A methylation state. Here, we systematically identified MeCP2 as a pro-metastasis gene to regulate m A methylation in colorectal cancer. Interestingly, MeCP2 could bind to methyltransferase-like 14 (METTL14) to co-regulate tumor suppressor Kruppel like factor 4 (KLF4) expression through changing m A methylation modification. Furthermore, Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) recognized the unique modified m A methylation sites to enhance KLF4 mRNA stability. Taken together, these findings highlight the novel function of MeCP2 for regulating m A methylation and demonstrated the underlying molecular mechanism for the interaction between MeCP2 and METTL14, which offers a better understanding of CRC progression and metastasis.
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http://dx.doi.org/10.1111/cas.15011DOI Listing
June 2021

Androgen Receptor, Although Not a Specific Marker For, Is a Novel Target to Suppress Glioma Stem Cells as a Therapeutic Strategy for Glioblastoma.

Front Oncol 2021 21;11:616625. Epub 2021 May 21.

Department of Radiation Oncology, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, United States.

Targeting androgen receptor (AR) has been shown to be promising in treating glioblastoma (GBM) in cell culture and flank implant models but the mechanisms remain unclear. AR antagonists including enzalutamide are available for treating prostate cancer patients in clinic and can pass the blood-brain barrier, thus are potentially good candidates for GBM treatment but have not been tested in GBM orthotopically. Our current studies confirmed that in patients, a majority of GBM tumors overexpress AR in both genders. Enzalutamide inhibited the proliferation of GBM cells both and . Although confocal microscopy demonstrated that AR is expressed but not specifically in glioma cancer stem cells (CSCs) (CD133+), enzalutamide treatment significantly decreased CSC population in cultured monolayer cells and spheroids, suppressed tumor sphere-forming capacity of GBM cells, and downregulated CSC gene expression at mRNA and protein levels in a dose- and time-dependent manner. We have, for the first time, demonstrated that enzalutamide treatment decreased the density of CSCs and improved survival in an orthotopic GBM mouse model. We conclude that AR antagonists potently target glioma CSCs in addition to suppressing the overall proliferation of GBM cells as a mechanism supporting their repurposing for clinical applications treating GBM.
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http://dx.doi.org/10.3389/fonc.2021.616625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175980PMC
May 2021

Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult.

Front Immunol 2021 20;12:649285. Epub 2021 May 20.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Heterotopic ossification (HO) is one of the most intractable disorders following musculoskeletal injury and is characterized by the ectopic presence of bone tissue in the soft tissue leading to severe loss of function in the extremities. Recent studies have indicated that immune cell infiltration and inflammation are involved in aberrant bone formation. In this study, we found increased monocyte/macrophage and mast cell accumulation during early HO progression. Macrophage depletion by clodronate liposomes and mast cell stabilization by cromolyn sodium significantly impeded HO formation. Therefore, we proposed that the dietary phytochemical quercetin could also suppress immune cell recruitment and related inflammatory responses to prevent HO. As expected, quercetin inhibited the monocyte-to-macrophage transition, macrophage polarization, and mast cell activation in a dose-dependent manner. Using a murine burn/tenotomy model, we also demonstrated that quercetin attenuated inflammatory responses and HO . Furthermore, elevated SIRT1 and decreased acetylated NFκB p65 expression were responsible for the mechanism of quercetin, and the beneficial effects of quercetin were reversed by the SIRT1 antagonist EX527 and mimicked by the SIRT agonist SRT1720. The findings in this study suggest that targeting monocyte/macrophage and mast cell activities may represent an attractive approach for therapeutic intervention of HO and that quercetin may serve as a promising therapeutic candidate for the treatment of trauma-induced HO by modulating SIRT1/NFκB signaling.
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http://dx.doi.org/10.3389/fimmu.2021.649285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173182PMC
May 2021

Improvement in Midline Shift Is a Positive Prognostic Predictor for Malignant Middle Cerebral Artery Infarction Patients Undergoing Decompressive Craniectomy.

Front Neurol 2021 20;12:652827. Epub 2021 May 20.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

The aim of this retrospective study is to evaluate the risk factors of malignant middle cerebral artery infarction (MMCAI) patients and explore an applicable prognostic predictor for MMCAI patients undergoing decompressive craniectomy (DC). Clinical data from the period 2012-2017 were retrospectively evaluated. Forty-three consecutive MMCAI patients undergoing DC were enrolled in this study. The 30-day mortality was assessed, and age, location, hypertension, pupil dilation, onset to operation duration, midline shift, and Glasgow Coma Scale (GCS) score were identified by univariate analysis and binary logistic regression. In this retrospective study for DC patients, the 30-day mortality was 44.2%. In the univariate analysis, advanced age (≥60 years), right hemispheric location, hypertension, pupil dilation, shorter onset to operation duration (<48 h), improved midline shift ( = 4.214, < 0.01), and lower pre-operation GCS score were significant predictors of death within 30 days. In binary logistic regression analysis, age [odds ratio (OR) = 1.141, 95% CI 1.011-1.287], the improvement of the midline shift (OR = 0.764, 95% CI 0.59-0.988), and pupillary dilation (OR = 15.10, 95% CI 1.374-165.954) were independent influencing factors. For the receiver operating characteristic (ROC) analysis of the relationship between post-operation outcomes and midline shift improvement, the area under the curve (AUC) was 0.844, and the cutoff point of midline shift improvement was 0.83 cm. Improved midline shift was a significant predictor of 30-day mortality. The improved midline shift of >0.83 cm indicated survival at 30 days.
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http://dx.doi.org/10.3389/fneur.2021.652827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176305PMC
May 2021

Circular RNA circDUS2 Is a Potential Biomarker for Intracranial Aneurysm.

Front Aging Neurosci 2021 19;13:632448. Epub 2021 May 19.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

CircRNAs have been found to play a crucial role in the pathological process of various kinds of diseases. However, the role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown. Differentially expressed circRNAs profiles between superficial temporal arteries ( = 5) and intracranial aneurysms ( = 5) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was utilized to validate the differential expression of circDUS2. Fluorescence hybridization (FISH) was meant for the location of circDUS2 in human brain vascular smooth muscle cell (HBVSMC). Structural analysis was used to speculate on the function of circDUS2. Five hundred forty-three upregulated and 397 downregulated significantly in intracranial aneurysm as compared to superficial temporal arteries. Quantitative real-time PCR verified the elevated expression of the upregulated circDUS2. The FISH test revealed that circDUS2 is located in the cytoplasm of brain vascular smooth muscle cells. This study showed differential expression data of circRNAs between superficial temporal artery and intracranial aneurysm and revealed that circDUS2 is a potential molecular marker for intracranial aneurysm.
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http://dx.doi.org/10.3389/fnagi.2021.632448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171118PMC
May 2021

Maillard reaction products and Guaiacol as production process and raw material markers for the authenticity identification of sesame oil.

J Sci Food Agric 2021 Jun 6. Epub 2021 Jun 6.

The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, 430074, PR China.

Background: Sesame oil has excellent flavor and is widely loved by people. Sesame oil has a higher price than other vegetable oils because of its high raw material price, and different processing techniques also result in different quality and price. In the market, driven by economic interests, the problem of adulteration of sesame oil is endless. Therefore, screening of volatile markers used to distinguish the authenticity of sesame oil raw materials and processes is of great significance.

Results: In this work, 6 markers related to the production processes and raw materials of sesame oil were screened by GC-MS/MS combined with chemometric, including 3-methyl-2-butanone, 2-ethyl-5-methyl-pyrazine, guaiacol, 2,6-dimethyl-pyrazine, 5-methyl furfural, and ethyl-pyrazine. The concentration of these markers in sesame oil is 10-1000 times that of other vegetable oils. However, only 3-methyl-2-butanone and 2-ethyl-5-methyl-pyrazine were significant differences during different production processes. Except for guaiacol, mainly derived from raw materials, the other five compounds are all from the Maillard reaction during thermal processing. Combined with discriminatory analysis results, just these six compounds can accurately distinguish sesame oil raw materials and process fraud and accurately identify 30% adulteration concentration.

Conclusion: In this study, the classification markers can accurately identify the raw material and process adulteration of sesame oil. Therefore, these six compounds are of great significance to the authenticity of sesame oil and provide a theoretical basis for the rapid and accurate identification of the authenticity of sesame oil. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11353DOI Listing
June 2021

A comprehensive exploration on pollution characteristics and health risks of potentially toxic elements in indoor dust from a large Cu smelting area, Central China.

Environ Sci Pollut Res Int 2021 Jun 5. Epub 2021 Jun 5.

State Environmental Protection Key Laboratory of Urban Environment and Ecology, Ministry of Ecology and Environment, South China Institute of Environmental Sciences, Guangzhou, 510655, China.

Large-scale smelting activities release large amounts of potentially toxic elements (PTEs) in fine particles. These particles floating in the air eventually settle on leaves, roads, and even indoors. In smelting areas, indoor environments are generally considered relatively safe. However, these areas are not taken seriously and need to be assessed. This paper systematically studied pollution characteristics, main sources and health risks of ten potentially toxic elements, PTEs (Mn, Ni, Cu, Zn, Hg, Cd, As, Cr, Pb, and Tl), of dust samples from different indoor environments in smelting areas using various methods. Therefore, this study analyzed dust samples from 35 indoor environments. The enrichment factors showed that the indoor dust samples were extremely enriched by Cd and Cu and significantly enriched by Hg, Pb, As, and Zn. The result of the spatial distribution showed that the high-value PTEs were mainly distributed near the Cu smeltery. Three sources were quantitatively assigned for these PTEs, and they were industrial smelting and traffic activities (44.40%), coal-fired activities (18.11%), and natural existence (37.49%). Based on the calculation of health risk, the value of THI for children was 7.57, indicating a significant non-carcinogenic risk. For carcinogenic risk, the values of TCR for children and adults were 2.91×10 and 2.97×10, respectively, which were much higher than the acceptable risk value 1×10. Combining health risk assessment with source discrimination, we found that the industrial discharges and traffic activities were the most main source of non-cancer and cancer risks. Therefore, smelting activities should be more strictly monitored, and traffic emission management should be strengthened.
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http://dx.doi.org/10.1007/s11356-021-14724-6DOI Listing
June 2021

Intermittent administration of a fasting-mimicking diet reduces intestinal inflammation and promotes repair to ameliorate inflammatory bowel disease in mice.

J Nutr Biochem 2021 Jun 1:108785. Epub 2021 Jun 1.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China, 200031; School of Life Science and Technology, ShanghaiTech University, Shanghai, China, 201210. Electronic address:

Recent studies have revealed that calorie restriction is able to modulate immune system and aid in intervention of immune disorders. Inflammatory bowel disease (IBD) is an immune disease in the intestine caused by interplay between genetic susceptibility and environmental factors such as diets. Here we analyzed the therapeutic effect of intermittent calorie restriction with a fasting-mimicking diet (FMD) on dextran sodium sulfate (DSS)-induced chronic IBD model in mice. Two cycles of FMD was administered after IBD symptoms occurred in the mice. FMD administration significantly reduced the score of disease activity index. FMD reversed DSS-mediated shortening of colon length, infiltration of lymphocytes in the crypt of colon, and accumulation of CD4 cells in the colon and small intestine. The expression of an inflammation marker NLRP3 was also reduced by FMD administration. The percentage of CD4 T cells in both peripheral blood and spleen was also reduced by FMD. In addition, FMD application reversed DSS-mediated reduction in intestinal stem cell marker Lgr5, while the cell proliferation markers Ki67 and PCNA were increased by FMD. Taken together, these results indicate that in the mouse model of IBD, application of the FMD can effectively ameliorate the symptoms and pathogenesis of IBD through reducing the inflammation of intestine and promoting the regeneration and repair of the damaged intestinal epithelium.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108785DOI Listing
June 2021

The biomechanical influence of facet joint parameters on corresponding segment in the lumbar spine: A new visualization method.

Spine J 2021 May 30. Epub 2021 May 30.

Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, the People's Republic of China; Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases, Liaoning Province, the People's Republic of China. Electronic address:

Background Context: Facet joints have been discussed as influential factors in the development of lumbar degeneration, which includes disc herniation and degenerative lumbar spondylolisthesis. Facet orientation (FO) and facet tropism (FT) are two important structural parameters of the lumbar facet joints. Many previous studies have focused on single parameter analysis of the lumbar spine. Owing to the correlation between independent variables, single-factor analysis cannot reflect the interaction between variables; however, there has been no corresponding biomechanical method developed to address this problem.

Purpose: To investigate the complex biomechanical influences on the lumbar spine when vertebral FO and FT are varied using finite element analysis (FEA) and contour maps visualization, and analyze the biomechanical role of facet joint structural parameters in the process of lumbar degenerative diseases.

Study Design: A biomechanical modelling, analysis, and verification study was performed.

Methods: A three-dimensional non-linear FEA model of 3 denucleated intervertebral discs (L2-3, L3-4, L4-5) with adjacent vertebral bodies (L2-L5) was created. Previously performed in vitro experiments provided experimental data for the range of motion in each load direction that was used for calibration. For 12 lumbar models, different facet joint angles relative to the sagittal plane at both L3-4 facet joints were simulated for 35°≤FO≤50° and 0°≤FT≤15°. By modifying different values of FO and FT, FEA simulation of different lumbar spine models was performed. Contour maps were used to visualize the FO- and FT-relevant data.

Results: Under flexion, extension, and torsion moments, facet joint contact force and intradiscal stress increased with increasing FT. In the condition where FT remained 0° and increasing FO values, facet joint contact force and intradiscal stress remained low with no apparent increasing or decreasing trend when the model was under flexion, extension, and torsion moments. In the condition where FO and the FT values were varied at the same time, the highest force and stress regions in the contour maps were observed when all three types of moments were applied. Stress distributions of the L3-4 disc with different FT and FO values showed disc stress increased significantly with increases of FT and was concentrated on the ipsilateral region of the facet joint with the more sagittal orientation.

Conclusions: The combination of FO and FT has an important impact on the corresponding disc and facet joints, but FT played a more significant role. Moreover, disc stress was concentrated on the ipsilateral region of facet joint with greater sagittal orientation when FT existed. FT with high sagittal orientation may increase risk of recurrent LDH due to increase ipsilateral disc pressure.

Clinical Significance: These biomechanical findings may help clinicians to understand the prognosis of some lumbar degenerative conditions.
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http://dx.doi.org/10.1016/j.spinee.2021.05.024DOI Listing
May 2021

Mix Contrast for COVID-19 Mild-to-critical Prediction.

IEEE Trans Biomed Eng 2021 Jun 1;PP. Epub 2021 Jun 1.

Objective: In a few patients with mild COVID-19, there is a possibility of the infection becoming severe or critical in the future. This work aims to identify high-risk patients who have a high probability of changing from mild to critical COVID-19 (only account for 5% of cases). Methods: Using traditional convolutional neural networks for classification may not be suitable to identify this 5% of high-risk patients from an entire dataset due to the highly imbalanced label distribution. To address this problem, we propose a Mix Contrast (MixCo) model, which matches original features with mixed features for contrastive learning. Three modules are proposed for training MixCo: 1) a cumulative learning strategy for synthesizing the mixed feature; 2) a commutative feature combination module for learning the commutative law of feature concatenation; 3) a united pairwise loss assigning adaptive weights for sample pairs with different class anchors based on their current optimization status. Results: We collect a multi-center computed tomography dataset including 918 confirmed COVID-19 patients from four hospitals and evaluate the proposed method on both the COVID-19 mild-to-critical prediction and COVID-19 diagnosis tasks. For mild-to-critical prediction, the experimental results show a recall of 0.80 and a specificity of 0.815. For diagnosis, the model shows comparable results with deep neural networks using a large dataset. Our method demonstrates improvements when the amount of training data is small or imbalanced. Significance: Identifying mild-to-critical COVID-19 patients is important for early prevention and personalized treatment planning.
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http://dx.doi.org/10.1109/TBME.2021.3085576DOI Listing
June 2021

Evidence accumulation relates to perceptual consciousness and monitoring.

Nat Commun 2021 05 31;12(1):3261. Epub 2021 May 31.

Laboratory of Cognitive Neuroscience, Center for Neuroprosthetics and Brain Mind Institute, Faculty of Life Sciences, Swiss Federal Institute of Technology (EPFL), Geneva, Switzerland.

A fundamental scientific question concerns the neural basis of perceptual consciousness and perceptual monitoring resulting from the processing of sensory events. Although recent studies identified neurons reflecting stimulus visibility, their functional role remains unknown. Here, we show that perceptual consciousness and monitoring involve evidence accumulation. We recorded single-neuron activity in a participant with a microelectrode in the posterior parietal cortex, while they detected vibrotactile stimuli around detection threshold and provided confidence estimates. We find that detected stimuli elicited neuronal responses resembling evidence accumulation during decision-making, irrespective of motor confounds or task demands. We generalize these findings in healthy volunteers using electroencephalography. Behavioral and neural responses are reproduced with a computational model considering a stimulus as detected if accumulated evidence reaches a bound, and confidence as the distance between maximal evidence and that bound. We conclude that gradual changes in neuronal dynamics during evidence accumulation relates to perceptual consciousness and perceptual monitoring in humans.
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http://dx.doi.org/10.1038/s41467-021-23540-yDOI Listing
May 2021

Diagnostic Value of Diurnal Variability of Orthostatic Heart Rate Increment in Children and Adolescents With POTS.

Front Pediatr 2021 13;9:644461. Epub 2021 May 13.

Department of Pediatric Cardiovasology, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.

This study aims to investigate the diurnal variability of heart rate (HR) increment after standing (ΔHR) in pediatric postural tachycardia syndrome (POTS) and explore appropriate cutoff values of ΔHR at different times for the POTS diagnosis. Seventy-eight patients (9-14 years) who presented with orthostatic intolerance symptoms were enrolled. Forty-three patients were diagnosed as POTS (ΔHR ≥40 bpm), and 35 patients were assigned to the non-POTS group (ΔHR <40 bpm). Twenty-six healthy children served as the control group. All subjects completed three standing tests in the morning, afternoon, and evening. Orthostatic HR parameters were analyzed to predict the diagnosis of POTS. Additionally, 41 patients were recruited as an external validation group. Orthostatic HR increments in both the POTS and non-POTS groups exhibited diurnal variability, which was markedly larger in the morning ( < 0.05), whereas it did not differ with the time of day in the control group. Among the POTS patients, 100% met the diagnostic criteria for POTS in the morning, 44.2% in the afternoon, and 27.9% in the evening. Almost half of the POTS patients (51.2%) displayed a positive result only in the morning standing test. However, in the three standing tests at different times, ΔHR from 1 to 10 min after standing and ΔHR were the highest in the POTS group compared with in the non-POTS and control groups ( < 0.05). Furthermore, the maximum ΔHR (ΔHR) and ΔHR at 5 and 10 min in the afternoon and evening standing tests yielded moderate predictive values for the POTS diagnosis. The external validation test showed that the afternoon ΔHR ≥30 bpm to diagnose POTS yielded sensitivity, specificity, and accuracy of 85, 71.4, and 78%, respectively, and the evening ΔHR ≥25 bpm yielded sensitivity, specificity, and accuracy of 85, 76.2, and 80.5%, respectively. The orthostatic HR increment exhibits diurnal variability in children and adolescents with POTS that may affect the diagnosis of POTS. Supplementary criteria are proposed for the POTS diagnosis based on diurnal variability.
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http://dx.doi.org/10.3389/fped.2021.644461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157922PMC
May 2021

Viral Gene Therapy for Glioblastoma Multiforme: A Promising Hope for the Current Dilemma.

Front Oncol 2021 13;11:678226. Epub 2021 May 13.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Glioblastoma multiforme (GBM), as one of the most common malignant brain tumors, was limited in its treatment effectiveness with current options. Its invasive and infiltrative features led to tumor recurrence and poor prognosis. Effective treatment and survival improvement have always been a challenge. With the exploration of genetic mutations and molecular pathways in neuro-oncology, gene therapy is becoming a promising therapeutic approach. Therapeutic genes are delivered into target cells with viral vectors to act specific antitumor effects, which can be used in gene delivery, play an oncolysis effect, and induce host immune response. The application of engineering technology makes the virus vector used in genetics a more prospective future. Recent advances in viral gene therapy offer hope for treating brain tumors. In this review, we discuss the types and designs of viruses as well as their study progress and potential applications in the treatment of GBM. Although still under research, viral gene therapy is promising to be a new therapeutic approach for GBM treatment in the future.
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http://dx.doi.org/10.3389/fonc.2021.678226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155537PMC
May 2021

LncRNA PCA3 promotes antimony-induced lipid metabolic disorder in prostate cancer by targeting MIR-132-3 P/SREBP1 signaling.

Toxicol Lett 2021 May 28;348:50-58. Epub 2021 May 28.

Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China; The School of Medicine, Nankai University, Tianjin, China. Electronic address:

Antimony is a common environmental contaminant that causes biological toxicity in exposed populations worldwide. Previous studies have revealed that antimony promotes prostate cancer growth by stabilizing the c-Myc protein and mimicking androgen activity. However, the role of lncRNAs in the regulation of antimony-induced carcinogenesis remains unknown, and the precise mechanisms need to be explored. In the present study, we found that chronic exposure to antimony promoted cell growth and lipid metabolic disequilibrium in prostate cancer. Mechanistically, we identified a long noncoding RNA molecule, PCA3, that was substantially upregulated in LNCaP cells in response to long-term antimony exposure. Functional studies indicated that abnormal PCA3 expression modulated antimony-induced proliferation and cellular triglyceride and cholesterol levels. In addition, PCA3 levels were found to be inversely correlated with MIR-132-3 P levels by acting as a decoy for MIR-132-3P. Besides, SREBP1 directly interacted with MIR-132-3 P to increase cell growth and disrupt lipid metabolism by targeting its 3'UTR regions. Taken together, our results revealed that lncRNA PCA3 promotes antimony-induced lipid metabolic disorder in prostate cancer by targeting MIR-132-3 P/SREBP1 signaling.
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http://dx.doi.org/10.1016/j.toxlet.2021.05.006DOI Listing
May 2021

LncRNA OIP5-AS1 inhibits ferroptosis in prostate cancer with long-term cadmium exposure through miR-128-3p/SLC7A11 signaling.

Ecotoxicol Environ Saf 2021 May 26;220:112376. Epub 2021 May 26.

Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, PR China; The School of Medicine, Nankai University, Tianjin 300071, PR China. Electronic address:

Previous studies suggest that cadmium (Cd) is one of the causative factors of prostate cancer (PCa), but the effect of chronic Cd exposure on PCa progression remains unclear. Besides, whether long noncoding RNAs (lncRNAs) are involved in the regulation of prolonged exposure to Cd in PCa needs to be elucidated. In the present study, we found that the serum concentration of Cd in PCa patients was positively correlated with the Gleason score and tumor-node-metastasis (TNM) classification. To simulate chronic Cd exposure in PCa, we subjected PC3 and DU145 cells to long-term, low-dose Cd exposure and further examined tumor behavior. Functional studies identified that chronic Cd exposure promoted cell growth and ferroptosis resistance in vitro and in vivo. Furthermore, we found that lncRNA OIP5-AS1 expression was greatly elevated in PC3 and DU145 cells upon chronic Cd exposure. Dysregulation of OIP5-AS1 expression mediated cell growth and Cd-induced ferroptosis. Mechanistically, we demonstrated that OIP5-AS1 served as an endogenous sponge of miR-128-3p to regulate the expression of SLC7A11, a surrogate marker of ferroptosis. Moreover, miR-128-3p decreased cell viability by enhancing ferroptosis. Taken together, our data indicate that lncRNA OIP5-AS1 promotes PCa progression and ferroptosis resistance through miR-128-3p/SLC7A11 signaling.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112376DOI Listing
May 2021

Physical activity and sleep duration during pregnancy have interactive effects on caesarean delivery: a population-based cohort study in Tianjin, China.

BMC Pregnancy Childbirth 2021 May 28;21(1):406. Epub 2021 May 28.

Department of Epidemiology and Biostatistics, School of Public Health, Tianjin Medical University, P.O. Box 154, 22 Qixiangtai Road, Heping District, Tianjin 300070, China.

Background: There were inconsistent findings in the literature regarding the associations of physical activity and sleep duration during pregnancy with caesarean delivery for different reasons. It was also unknown whether physical activity and sleep duration during pregnancy had interactive effects on the risks of different types of caesarean delivery. The study aimed to investigate the effects of physical activity, sleep duration and their interactions on the risk of caesarean delivery for medical reasons and non-medical reasons.

Methods: From October 2010 to August 2012, a prospective population-based cohort of 13,015 pregnant women was established in six central urban districts of Tianjin, China. Pregnancy outcomes were retrieved from an electronic database and caesarean delivery was divided into caesarean delivery for medical reasons and caesarean delivery for non-medical reasons. Physical activity and sleep status were collected at 24-28 weeks of gestation using self-reported questionnaires. Logistic regression and additive interaction were used to examine physical activity, sleep duration and their interactive effects on risk of caesarean delivery.

Results: In the cohort, 5692 (43.7%) and 2641 (20.3%) of women had caesarean delivery for medical reasons and non-medical reasons, respectively. Low physical activity increased the risk of caesarean delivery for medical reasons (adjusted OR: 1.13, 95%CI 1.04-1.23) but not caesarean delivery for non-medical reasons. Sleep duration < 7 h/day and poor sleep quality were not associated with caesarean delivery. Sleep duration ≥9 h/day increased the risk of caesarean delivery for medical reasons (1.12, 1.02-1.22) and caesarean delivery for non-medical reasons (1.16, 1.05-1.29). Co-presence of low physical activity and sleep duration ≥9 h/day increased risk of caesarean delivery (1.25, 1.12-1.41), and their additive interaction was statistically significant for caesarean delivery for medical reasons but not for caesarean delivery for non-medical reasons.

Conclusions: Low physical activity and excessive sleep duration during pregnancy each increased the risk of caesarean delivery, and they had an interactive effect on the risk of caesarean delivery for medical reasons but not on the risk of caesarean delivery for non-medical reasons. Increasing physical activity and maintaining recommended sleep duration during pregnancy may have benefits for perinatal health.
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http://dx.doi.org/10.1186/s12884-021-03788-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161996PMC
May 2021

The effect and pharmacology of Yizhi Sheng Hui decoction on Alzheimer's disease.

Ann Palliat Med 2021 May 14;10(5):5244-5251. Epub 2021 May 14.

The Fourth Department of Healthcare, China-Japan Friendship Hospital, Beijing, China.

Background: Alzheimer's disease (AD) has gradually increased as society has aged and is now a serious social problem. In the clinical treatment of AD, patients show improvement in cognitive function after treatment with the traditional Chinese medicine compound, Yizhi Sheng Hui (YZSH) decoction. This study systematically investigates the effect and pharmacology of the YZSH decoction on AD.

Methods: In this study, 24 SAMP8 AD model mice were randomly divided into three groups: an untreated control group, a group treated with the YZSH decoction, and a positive control group treated with donepezil hydrochloride. Eight SAMR1 mice were placed in the normal control group. A Morris water maze test and a step-down test were conducted at 8 and 13 weeks after continuous intragastric administration of the two drugs. After 13 weeks of administration, the hippocampal expression of Aβ1-42 and tau protein were measured.

Results: There was no change in the latent period duration and the number of platform crossings in each group after 8 weeks of administration, but after 13 weeks of administration, the latent period of the treatment group and the positive control group were significantly shorter than the untreated control group. Initially, the SAMP8 mice showed a lower spatial exploration ability than the SAMR1 mice. However, after 13 weeks of administration, the treatment group and the control group exhibited a better exploration ability. Compared with the SAMR1 mice, the on-stage evasion time and step-down errors significantly increased in the untreated group. Compared with the untreated group, mice in the treatment group and the positive control group showed a shorter latent period after 8 weeks of administration, and the on-stage evasion time for both groups was significantly reduced after 13 weeks of administration. The treatment group showed fewer instances of electric shock. Hippocampal expression of Aβ1-42 was high in the untreated group, was much lower in the positive control group, and no Aβ1-42 expression was observed in the treatment group.

Conclusions: The YZSH decoction improved the learning and memory of mice with AD, related to the inhibition of Aβ1-42 expression.
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http://dx.doi.org/10.21037/apm-20-1629DOI Listing
May 2021

Hollow-Sphere-Structured NaFe(PO)(PO)/C as a Cathode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):25972-25980. Epub 2021 May 26.

CAS Key Laboratory of Materials for Energy Conversions, Department of Materials Science and Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, University of Science and Technology of China, Hefei, Anhui 230026, China.

The mixed polyanionic material NaFe(PO)(PO) combines the advantages of NaFePO and NaFePO in capacity, stability, and cost. Herein, we synthesized carbon-coated hollow-sphere-structured NaFe(PO)(PO) powders by a scalable spray drying route. The optimal sample can deliver a high discharge capacity of 107.7 mA h g at 0.2C. It also delivers a capacity of 88 mA h g at 10C and a capacity of retention of 92% after 1500 cycles. Ex situ X-ray diffraction analysis indicates a slight volume change (less than 3%) in the NaFe(PO)(PO) lattice cell. Therefore, such a spraying-derived carbon-coated NaFe(PO)(PO) powder is a very attractive cathode electrode for sodium-ion batteries.
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http://dx.doi.org/10.1021/acsami.1c04035DOI Listing
June 2021

A facile aptasensor based on polydopamine nanospheres for high-sensitivity sensing of T-2 toxin.

Anal Methods 2021 May 26. Epub 2021 May 26.

Ministry of Education, College of Food Science, Southwest University, Chongqing 400715, P. R. China. and Medical College, Nankai University, Tianjin 300457, P. R. China.

A facile fluorescent aptasensor based on polydopamine nanospheres (PDANSs) has been proposed for the rapid and high sensitive sensing of T-2 toxin. PDANSs are dopamine-derived synthetic eumelanin polymers with excellent fluorescence quenching ability, dispersibility and biocompatibility. In the assay, 6-carboxyfluorescein (FAM)-labeled aptamers (FAM-aptamers) were adsorbed onto PDANSs via noncovalent bonding, resulting in quenching fluorescence. In the presence of T-2, the binding of T-2 to the aptamers could promote the formation of the A-form duplex hairpin structure, which was used as a sensing platform to detect T-2 on the basis of fluorescence recovery. The results showed that the aptasensor was rapid and sensitive for the detection of T-2 toxin with a linear detection range of 10-180 μg L-1 and a detection limit of 7.23 μg L-1. The performance of the proposed method was comparable with that of the liquid chromatography-mass spectrometry method (LC-MS). Thus, the aptasensor could be used for the determination of real samples. The design method proposed in this study provides a strategy for the development of PDANS-based toxin biosensors.
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http://dx.doi.org/10.1039/d1ay00642hDOI Listing
May 2021

Thermal conductivity and mechanical performance of hexagonal boron nitride nanosheets-based epoxy adhesives.

Nanotechnology 2021 Jun 11;32(35). Epub 2021 Jun 11.

College of Aerospace Engineering, Shenyang Aerospace University, Shenyang 110136, People's Republic of China.

Thermosets possess diverse physical and chemical properties and thus they are widely used in various applications such as electronic packaging, construction, and automotive industries. However, their poor thermal conductivity and weak mechanical performance jeopardize their continual spread in modern industry. In this study, boron nitride nanosheets (BNNSs) were employed to promote both mechanical and thermal properties of epoxy nanocomposites. BNNSs and their epoxy nanocomposites were fabricated usingsolvent ultrasonication andpolymerization, respectively. Thermal conductivity was enhanced by 153% increment in epoxy/BNNS nanocomposite at 7 wt% in comparison with neat epoxy. In parallel, Young's modulus, lap shear strength, fracture toughness () and energy release rate () increased by 69%, 31%, 122% and 118%, respectively at 1 wt% BNNSs. Moreover, fatigue life and strength of lap shear joints were significantly improved upon adding BNNSs. A numerical model of the single lap shear joint was developed to validate the accuracy of the material constants obtained. Epoxy/BNNS nanocomposites exhibited an outstanding mechanical performance as well as high thermal conductivity giving them merits to widen their applications in electronic and automotive industry.
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http://dx.doi.org/10.1088/1361-6528/ac0470DOI Listing
June 2021

Novel AgPO/boron-carbon-nitrogen photocatalyst for highly efficient degradation of organic pollutants under visible-light irradiation.

J Environ Manage 2021 Aug 19;292:112763. Epub 2021 May 19.

Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44, Leuven, 3001, Belgium. Electronic address:

AgPO is an indirect bandgap semiconductor with excellent photocatalytic activity. However, it has not been widely used so far for the treatment of polluted wastewaters. This scarce use in wastewater treatment can be mainly attributed to its large crystallite size, which would be due to rapid agglomeration during the synthesis process, as well as to the photo-corrosion problem affecting this material. Hence, it would be crucial to develop a photocatalytic system involving AgPO nanoparticles with enhanced properties, such as higher specific surface area and excellent photocatalytic stability. To meet this demand, a novel AgPO/boron carbon nitrogen (AgPO/BCN) composite photocatalyst was successfully prepared in the present study via electrostatically driven self-assembly and ion exchange processes. After characterization and assessment, it was shown that the as-prepared AgPO/BCN nanocomposite photocatalyst not only contains smaller AgPO nanoparticles, but also exhibits an enhanced visible-light photocatalytic activity for Rhodamine B (RhB) Methyl Orange (MO) and Tetracycline (TC) and improved stability, without decrease after 5 cycles, compared with pure AgPO nanoparticles. Positive synergy between AgPO nanoparticles and BCN nanosheets, including the increase in the number of active adsorption sites, and the restriction of the formation of Ag due to the recombination of photogenerated electron-hole pairs in AgPO nanoparticles, are mainly responsible for the enhanced properties of the prepared catalyst. This study shows that AgPO/BCN composite photocatalyst would be promising for wastewater treatment, which would be of clearly environmental and public health relevance.
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http://dx.doi.org/10.1016/j.jenvman.2021.112763DOI Listing
August 2021

Newly Constructed NiCoO Derived from ZIF-67 with Dual Mimic Enzyme Properties for Colorimetric Detection of Biomolecules and Metal Ions.

ACS Appl Mater Interfaces 2021 Jun 21;13(21):25044-25052. Epub 2021 May 21.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, No.94 Weijin Road, Tianjin300071, China.

Integration of novel bio-/nanostructures as effective sensing platforms is still of great significance for robust and rapid analysis. Herein, a novel metal-organic framework-derived NiCoO was synthesized via a feasible templating method. Significantly, redox couples of both Ni/Ni and Co/Co provided richer oxidation-reduction reactions, thereby leading to an enhanced catalytic activity. Furthermore, NiCoO as an enzyme mimic with peroxidase-like activity and oxidase-like activity could oxidize colorless thylbenzidine (TMB) to blue oxTMB in the absence of HO. Thus, a sensitive chromogenic sensing platform for detecting Fe, thiourea, cysteine (Cys), and epigallocatechin-3-gallate (EGCG) was proposed. The colorimetric detection methods exhibited great features of low limit of detection (LOD) and broad linear range. Owing to the complexation reaction, the chromogenic sensing system of TMB + NiCoO + Cys achieved effective detection of Cu and Mn with the LODs of 0.0022 and 0.0181 mM, respectively. Developed detection methods with wide linear ranges of 0.008-0.1 mM for Cu and 0.08-1 mM for Mn had excellent practical potential. Similarly, the reaction system of TMB + NiCoO + EGCG could achieve the colorimetric detection of Cu and Fe. The great chromogenic sensing performance for detecting Cu and Fe with a broad linear range and a low LOD could be also realized.
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http://dx.doi.org/10.1021/acsami.1c06705DOI Listing
June 2021

Chlorogenic acid supplementation ameliorates hyperuricemia, relieves renal inflammation, and modulates intestinal homeostasis.

Food Funct 2021 May 21. Epub 2021 May 21.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China. and Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.

Hyperuricemia (HUA) is induced by abnormal purine metabolism and elevated serum uric acid (UA) concentrations, and it is often accompanied by inflammatory responses and intestinal disorders. This study aims to assess the protective effects of chlorogenic acid (CGA) on HUA in mice. CGA or allopurinol was given to mice with HUA induced by hypoxanthine and potassium oxonate. CGA lowered the levels of UA, blood urea nitrogen (BUN), creatinine (CR), AST, and ALT; inhibited xanthine oxidase (XOD) activity; and downregulated the mRNA expression of UA secretory proteins in HUA mice. Moreover, CGA significantly reduced serum lipopolysaccharides (LPS) levels and the mRNA expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, NOD-like receptor superfamily pyrin domain containing 3 (NLRP3), and caspase-1, and it inhibited the activation of the toll-like receptor 4/myeloid differentiation factor 88/nuclear factor kappa B (TLR4/MyD88/NF-κB) signaling pathway in the kidney, resulting in inflammation relief in HUA mice. In addition, CGA treatment increased the production of fecal short-chain fatty acids (SCFAs) in HUA mice. Additional investigations showed that CGA significantly lowered the mRNA expression of ileal IL-1β and IL-6, and it increased the mRNA expression of intestinal tight junction proteins (zonula occludens-1 (ZO-1) and occludin). Also, CGA increased the relative abundance of SCFA-producing bacteria, including Bacteroides, Prevotellaceae UGC-001, and Butyricimonas, and it reversed the purine metabolism and glutamate metabolism functions of gut microbiota. In conclusion, CGA may be a potential candidate for relieving the symptoms of HUA and regulating its associated inflammatory responses and intestinal homeostasis.
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http://dx.doi.org/10.1039/d0fo03199bDOI Listing
May 2021

Electrical Loss Management by Molecularly Manipulating Dopant-free Poly(3-hexylthiophene) towards 16.93% CsPbI2Br Solar Cells.

Angew Chem Int Ed Engl 2021 May 20. Epub 2021 May 20.

Institute of Chemistry, Chinese Academy of Sciences, CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, 2 North 1st St, Zhongguancun, 100190, Beijing, CHINA.

Inorganic cesium lead halide perovskites offer a pathway towards thermally stable photovoltaics. However, moisture-induced phase degradation restricts the application of hole transport layers (HTLs) with hygroscopic dopants. Dopant-free HTLs fail to realize efficient photovoltaics due to severe electrical loss. Herein, we developed an electrical loss management strategy by manipulating poly(3-hexylthiophene) with a small molecule, i.e., SMe-TATPyr. The developed P3HT/SMe-TATPyr HTL shows a three-time increase of carrier mobility owing to breaking the long-range ordering of "edge-on" P3HT and inducing the formation of "face-on" clusters, over 50% decrease of the perovskite surface defect density, and a reduced voltage loss at the perovskite/HTL interface because of favorable energy level alignment. The CsPbI 2 Br perovskite solar cell demonstrates a record-high efficiency of 16.93% for dopant-free HTL, and superior moisture and thermal stability by maintaining 96% efficiency at low-humidity condition (10%-25% R. H.) for 1500 hours and over 95% efficiency after annealing at 85 o C for 1000 hours.
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http://dx.doi.org/10.1002/anie.202105176DOI Listing
May 2021