Publications by authors named "Shuo Wang"

2,499 Publications

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A cucurbit[6]uril based supramolecular assembly for the detection and removal of dyes and antibiotics from water.

Anal Methods 2022 Jun 24. Epub 2022 Jun 24.

Department of Applied Chemistry, Yuncheng University, Yuncheng 044000, PR China.

A cucurbit[6]uril (CB[6]) based supramolecular assembly CB[6]-[NDS] (1, NDS = 2,6-naphthalenedisulfonic acid anion) was used to detect dyes such as reactive blue 19 (RB19), rhodamine B (RB), methyl orange (MO), methyl red (MR), and methyl violet (MV), and isoquinoline antibiotics such as berberine (BER) and palmatine (PAL) with detection limits of 143, 128, 374, 193, 305, 27 and 34 ppb, respectively. Simultaneously, 1 also displayed high adsorption abilities towards these organic molecules. These results indicate that 1 is a favorable material for the simultaneous selective detection and removal of specific dyes and antibiotics from water, being potentially useful in monitoring water quality and treating wastewater. The possible mechanisms of the detection and adsorption are also proposed.
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http://dx.doi.org/10.1039/d2ay00658hDOI Listing
June 2022

Exploring the Core Genes of Schizophrenia Based on Bioinformatics Analysis.

Genes (Basel) 2022 May 27;13(6). Epub 2022 May 27.

Clinical Research Center and Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.

Schizophrenia is a clinical syndrome composed of a group of symptoms involving many obstacles such as perception, thinking, emotion, behavior, and the disharmony of mental activities. Schizophrenia is one of the top ten causes of disability globally, accounting for about 1% of the global population. Previous studies have shown that schizophrenia has solid genetic characteristics. However, the diagnosis of schizophrenia mainly depends on symptomatic manifestations, and no gene can be used as a clear diagnostic marker at present. This study explored the hub genes of schizophrenia by bioinformatics analysis. Three datasets were selected and downloaded from the GEO database (GSE53987, GSE21138, and GSE27383). GEO2R, NCBI's online analysis tool, is used to screen out significant gene expression differences. The genes were functionally enriched by GO and KEGG enrichment analysis. On this basis, the hub genes were explored through Cytoscape software, and the immune infiltration analysis and diagnostic value of the screened hub genes were judged. Finally, four hub genes (NFKBIA, CDKN1A, BTG2, GADD45B) were screened. There was a significant correlation between two hub genes (NFKBIA, BTG2) and resting memory CD4 T cells. The ROC curve results showed that all four hub genes had diagnostic value.
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http://dx.doi.org/10.3390/genes13060967DOI Listing
May 2022

A New Subgenus and Species of from Mid-Cretaceous Kachin Amber (Coleoptera: Staphylinidae: Osoriinae).

Insects 2022 May 30;13(6). Epub 2022 May 30.

College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

As one of the largest families of beetles (Coleoptera), the Staphylinidae (rove beetles and their relatives) are rich not only in extant species but also in a comparatively robust fossil record. Despite this preponderance of available fossil material, fossils of the diverse subfamily Osoriinae remain rare. Here, we describe a new ososriine species, sp. nov., from the mid-Cretaceous amber of Myanmar. The new specimen is similar to the only other definitive fossil of the genus, Yamamoto 2019, and both are placed in the extinct subgenus subgen. nov. The new species differs noticeably in a number of morphological details in relation to the submentum, gular sutures and protibial crenulae. The new fossil provides further evidence for understanding the radiation of staphylinoid beetles.
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http://dx.doi.org/10.3390/insects13060513DOI Listing
May 2022

Germline Mutations in Patients With Early-Onset Prostate Cancer.

Front Oncol 2022 6;12:826778. Epub 2022 Jun 6.

Department of Urology, Daping Hospital, Army Medical University, Chongqing, China.

Objective: To investigate the inherited mutations and their association with clinical features and treatment response in young-onset prostate cancer patients.

Method: Targeted gene sequencing on 139 tumor susceptibility genes was conducted with a total of 24 patients diagnosed with PCa under the age of 63 years old. Meanwhile, the related clinical information of those patients is collected and analyzed.

Results: Sixty-two germline mutations in 45 genes were verified in 22 patients. (20.8%) and (20.8%) were found to be the most frequently mutated, followed by , and (8.3% of each, 2/24). Of note, 58.3% (14/24) patients carry germline mutations in DNA repair genes (DRGs). Four families with HRR (homologous recombination repair)-related gene mutations were described and analyzed in detail. Two patients with BRCA2 mutation responded well to the combined treatment of androgen deprivation therapy (ADT) and radiotherapy/chemotherapy.

Conclusion: Mutations in DRGs are more prevalent in early-onset PCa with advanced clinical stages, and these patients had shorter progression-free survival. ADT Combined with either radiotherapy or chemotherapy may be effective in treating PCa caused by HRR-related gene mutations.
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http://dx.doi.org/10.3389/fonc.2022.826778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207501PMC
June 2022

A Bioink Derived From Human Placenta Supporting Angiogenesis.

Biomed Mater 2022 Jun 22. Epub 2022 Jun 22.

Institute of Zoology Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District District, Beijing, 100101, CHINA.

Bioprinting is an emerging approach for constructing sophisticated tissue analogues with detailed architectures such as vascular networks, which requires bioink fulfill the highly printable property and provide a cell-friendly microenvironment mimicking native extracellular matrix (ECM). Here, we developed a human placental ECM-derived bioink (hp-bioink) meeting the requirements of 3D printing for printability and bioactivity. We first decellularized the human placenta, followed by enzymatic digestion, dialysis, lyophilization, and re-solubilization to convert the extracts into hp-bioink. Then, we demonstrated that 3%-5% of hp-bioink can be printed with self-standing and 1%-2% of hp-bioink can be embedded with suspended hydrogels. Moreover, hp-bioink supports HUVEC assembly in vitro and angiogenesis in mice in vivo. Our research enriched the bank of human-derived bioink, and provided a new opportunity to further accelerate bioprinting research and application.
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http://dx.doi.org/10.1088/1748-605X/ac7b5bDOI Listing
June 2022

Face identity coding in the deep neural network and primate brain.

Commun Biol 2022 Jun 20;5(1):611. Epub 2022 Jun 20.

Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, WV, 26506, USA.

A central challenge in face perception research is to understand how neurons encode face identities. This challenge has not been met largely due to the lack of simultaneous access to the entire face processing neural network and the lack of a comprehensive multifaceted model capable of characterizing a large number of facial features. Here, we addressed this challenge by conducting in silico experiments using a pre-trained face recognition deep neural network (DNN) with a diverse array of stimuli. We identified a subset of DNN units selective to face identities, and these identity-selective units demonstrated generalized discriminability to novel faces. Visualization and manipulation of the network revealed the importance of identity-selective units in face recognition. Importantly, using our monkey and human single-neuron recordings, we directly compared the response of artificial units with real primate neurons to the same stimuli and found that artificial units shared a similar representation of facial features as primate neurons. We also observed a region-based feature coding mechanism in DNN units as in human neurons. Together, by directly linking between artificial and primate neural systems, our results shed light on how the primate brain performs face recognition tasks.
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http://dx.doi.org/10.1038/s42003-022-03557-9DOI Listing
June 2022

Investigating binding mechanism of thymoquinone to human transferrin, targeting Alzheimer's disease therapy.

J Cell Biochem 2022 Jun 20. Epub 2022 Jun 20.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.

Iron deposition in the central nervous system (CNS) is one of the causes of neurodegenerative diseases. Human transferrin (hTf) acts as an iron carrier present in the blood plasma, preventing it from contributing to redox reactions. Plant compounds and their derivatives are frequently being used in preventing or delaying Alzheimer's disease (AD). Thymoquinone (TQ), a natural product has gained popularity because of its broad therapeutic applications. TQ is one of the significant phytoconstituent of Nigella sativa. The binding of TQ to hTf was determined by spectroscopic methods and isothermal titration calorimetry. We have observed that TQ strongly binds to hTf with a binding constant (K) of 0.22 × 10 M and forming a stable complex. In addition, isothermal titration calorimetry revealed the spontaneous binding of TQ with hTf. Molecular docking analysis showed key residues of the hTf that were involved in the binding to TQ. We further performed a 250 ns molecular dynamics simulation which deciphered the dynamics and stability of the hTf-TQ complex. Structure analysis suggested that the binding of TQ doesn't cause any significant alterations in the hTf structure during the course of simulation and a stable complex is formed. Altogether, we have elucidated the mechanism of binding of TQ with hTf, which can be further implicated in the development of a novel strategy for AD therapy.
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http://dx.doi.org/10.1002/jcb.30299DOI Listing
June 2022

The Relationship Between Unexplained Chest Pain in Children and Head-Up Tilt Test.

Front Pediatr 2022 2;10:901919. Epub 2022 Jun 2.

Department of Pediatric Cardiovasology, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: To explore the relationship between unexplained chest pain in children and head-up tilt test (HUTT).

Materials And Methods: A total of 825 children with the main symptom of unexplained chest pain were admitted to the Specialist Outpatient Clinic of Children's Cardiovascular Disease from October 2000 to November 2021 at The Second Xiangya Hospital, Central South University. Among them, 473 were male and 352 were female, with a mean age of 10.61 ± 2.21 years. The control group included 58 cases, comprising 35 males and 23 females, with a mean age of 10.26 ± 2.66 years. The detailed history, physical examinations, conventional 12-lead electrocardiogram, chest X-ray, echocardiography, myocardial enzymes, electroencephalogram, and blood series were all examined. Disorders of the chest wall, lung, heart, mediastinum, and esophageal reflux, as well as drug effects, were ruled out. All the children underwent HUTT. Demographic description, univariate analysis, and multivariate logistic regression analysis were used to explore the possible linear or non-linear relationships between the children's unexplained chest pain and HUTT.

Results: Among the 825 chest pain cases, 301 (36.48%) were HUTT positive and 524 (63.52%) were HUTT negative. HUTT-positive patients were older than HUTT-negative patients (11.04 ± 2.03 vs. 10.34 ± 2.31 years, < 0.001). The logistic regression results showed that each year of age increased the probability of being HUTT positive by 17.90% ( < 0.000), and females were 91.30% more likely to be HUTT positive than males ( < 0.000).

Conclusion: Clinically unexplained chest pain in children is mainly caused by unbalanced autonomic nervous function. HUTT can help clear the cause of unexplained chest pain.
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http://dx.doi.org/10.3389/fped.2022.901919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203148PMC
June 2022

Integrative Analysis of Metabolome and Transcriptome Reveals the Mechanism of Flavonoid Biosynthesis in Rehd.

ACS Omega 2022 Jun 31;7(23):19437-19453. Epub 2022 May 31.

College of Life Sciences, North China University of Science and Technology, Tangshan 063210, China.

Rehd has received great attention because of its pharmacological activities, such as inhibiting oxidation and lowering blood glucose and blood pressure, and flavonoids are one of its main pharmacodynamic components. It is important to understand the mechanisms of the flavonoid biosynthetic pathway of , but the regulation of flavonoid biosynthesis is still unclear. In this study, differentially expressed genes and differentially accumulated metabolites in were studied by integrating transcriptomics and metabolomics technologies. We confirmed the key genes involved in the flavonoid biosynthesis of , including , , , , and , which had consistent expression patterns with their upstream and downstream metabolites, and there is a significantly positive correlation between them. Compared to mature leaves, stems and young leaves are higher in the expression levels of key structural genes. We deduced that the MYB and bHLH transcription factors regulated the biosynthesis of different flavonoid metabolites and their regulatory patterns. Among them, , , , , and positively regulated the biosynthesis of flavones and flavanones. This discovery preliminarily revealed the pathways and key genes of flavonoid biosynthesis in , which provided a reference for further study on flavonoid biosynthesis.
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http://dx.doi.org/10.1021/acsomega.2c01125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202069PMC
June 2022

Impact of Persistent Poor Sleep Quality on Post-Stroke Anxiety and Depression: A National Prospective Clinical Registry Study.

Nat Sci Sleep 2022 13;14:1125-1135. Epub 2022 Jun 13.

Department of Neurology, Capital Medical University, Beijing, People's Republic of China.

Purpose: The impact of poor sleep quality after stroke, especially persistent poor sleep quality, on poststroke anxiety and depression is unclear. We seek to investigate the impact of baseline and persistent poor sleep quality on short-term poststroke anxiety and depression.

Patients And Methods: Data were analyzed for 1619 patients with acute ischemic stroke from the Impairment of Cognition and Sleep after Acute Ischemic Stroke or Transient Ischemic Attack in Chinese Patients study (ICONS). The sleep quality was assessed at 2 weeks and 3 months using the Pittsburgh Sleep Quality Index scale (PSQI). Poor sleep quality was defined as a PSQI score of >5, and persistent poor sleep quality was defined as a PSQI score of >5 at each time point. Patients were divided into three groups according to the quality of sleep: good sleep quality, baseline poor sleep quality and persistent poor sleep quality. Patient Health Questionnaire-9 (PHQ-9), General Anxiety Disorder-7 scale (GAD-7), and Modified Rankin Scale (mRS) at 3 months after stroke were taken as the study outcomes.

Results: Persistent poor sleep quality was present in 70.2% of patients after stroke. Compared to those with good sleep quality, patients with baseline poor sleep quality did not show significant differences in disability, anxiety and depression. However, patients with persistent poor sleep were at increased risk of depression (odds ratio, OR 3.04, 95% confidence interval, CI 1.66-5.57, P < 0.01) and anxiety (OR 3.20, 95% CI 1.42-7.19, P < 0.01) at 3 months after stroke. Persistent poor sleep quality was not identified as a risk factor for functional disability at 3 months.

Conclusion: Patients with persistent poor sleep quality are at added risks for depression and anxiety after stroke.
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http://dx.doi.org/10.2147/NSS.S357536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205438PMC
June 2022

The Values of Systemic Immune-Inflammation Index and Neutrophil-Lymphocyte Ratio in Predicting Biochemical Recurrence in Patients With Localized Prostate Cancer After Radical Prostatectomy.

Front Oncol 2022 2;12:907625. Epub 2022 Jun 2.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Urological Department, Peking University Cancer Hospital & Institute, Beijing, China.

Purpose: To investigate the association between preoperative systemic immune-inflammation index (SII) and neutrophil-lymphocyte ratio (NLR) and oncological outcomes in localized prostate cancer (PCa) patients after radical prostatectomy (RP).

Methods: Between January 2014 and December 2019, 291 patients with pathologically confirmed localized PCa who underwent RP were included in this study. The threshold values of SII and NLR for biochemical recurrence (BCR) were calculated according to Youden's index based on the receiver operating characteristic (ROC) curve, then the patients were divided into two groups by the threshold values of SII and NLR, and the clinicopathological outcomes were analyzed and compared between groups, respectively. The binary logistic regression model was used to evaluate the association between SII, NLR, and pathological outcomes including Gleason score (GS) and pathological T (pT) stage. Kaplan-Meier curves and univariable and multivariable Cox regression models were used to determine the association between high SII, high NLR, and BCR-free survival, respectively.

Results: The median follow-up time was 48 months (IQR 36-62), and 114 (39.18%) patients developed BCR. The AUC of SII for BCR was 0.813 ( < 0.001), with a threshold value of 528.54, a sensitivity of 72.9%, and a specificity of 76.3%; the AUC of NLR for BCR was 0.824 ( < 0.001), with a threshold value of 2.62, a sensitivity of 71.2%, and a specificity of 81.6%. Patients were divided into two groups according to the threshold values of SII and NLR, respectively. Patients in the high SII group had higher tPSA, GS, pT stage, and BCR rate than patients in the low SII group ( = 0.004, 0.04, 0.007, and <0.001, respectively), and patients in the high NLR group had higher tPSA, GS, pT stage, and BCR rate than patients in the low NLR group ( = 0.04, 0.02, 0.006, and <0.001, respectively). Multivariable logistic regression analysis revealed that high SII was significantly correlated with adverse pathological outcomes of GS (HR, 1.656; 95% CI, 1.00-2.742, = 0.042) and pT stage (HR, 1.478; 95% CI, 0.972-3.64, = 0.028); there was no association between high NLR and pathological events. Kaplan-Meier analysis showed significantly poorer BCR-free survival in patients with high SII or high NLR ( < 0.001 and <0.001, respectively). By using the multivariable Cox regression model, high SII (HR, 4.521; 95% CI, 2.262-9.037, < 0.001) and high NLR (HR, 4.787; 95% CI, 2.339-9.798, < 0.001) were both significant predictors of BCR after RP.

Conclusion: High SII was significantly related to unfavorable clinicopathological outcomes. High preoperative SII and NLR were related to higher BCR rate in localized PCa after RP, and they were all independent risk factors associated with shorter BCR-free survival. These two factors might provide promising and inexpensive methods for predicting clinical outcomes in patients with RP.
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http://dx.doi.org/10.3389/fonc.2022.907625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200963PMC
June 2022

Impact of Heating Temperature and Fatty Acid Type on the Formation of Lipid Oxidation Products During Thermal Processing.

Front Nutr 2022 2;9:913297. Epub 2022 Jun 2.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, China.

Thermal treatment of lipids rich in fatty acids contributes to the formation of lipid oxidation products (LOPs), which have potentially harmful effects on human health. This study included soybean oil (SO), palm oil (PO), olive oil (OO), and lard oil (LO) as the research objects, with an aim to investigate the impact of heating temperature and fatty acid type on the generation of LOPs (α-dicarbonyl compounds, malondialdehyde (MDA), α,β-unsaturated aldehydes, and 16 volatile aldehydes). Results showed that LOPs increased significantly ( < 0.05) with the increase in temperature (100 ~ 200°C). Furthermore, the amount of 2,3-butanedione (159.53 μg/g), MDA (3.15 μg/g), 4-hydroxy-hexenal (3.03 μg/g), 2-butenal (292.18%), 2-pentenal (102.26%), hexanal (898.72%), and 2,4-heptadienal (E, E) (2182.05%) were more at 200°C in SO rich in polyunsaturated fatty acids (PUFAs) than other oils. Results from heat map analysis indicated that the 2, 4-heptadienal, and glyoxal related to the myristic acid of oil. Moreover, the MDA was in close association with PUFAs. Based on the effect of temperature and fatty acid type on the generation of LOPs, this study could serve as a control method to reduce harmful LOPs.
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http://dx.doi.org/10.3389/fnut.2022.913297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201814PMC
June 2022

Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axis.

Microbiome 2022 Jun 16;10(1):94. Epub 2022 Jun 16.

Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.

Background: The gut microbiota is reportedly involved in the progression and chemoresistance of various human malignancies. However, the underlying mechanisms behind how it exerts some effect on prostate cancer, as an extra-intestinal tumor, in a contact-independent way remain elusive and deserve exploration. Antibiotic exposure, one of the various factors affecting the gut microbiota community and capable of causing gut dysbiosis, is associated with multiple disorders. This study aims to preliminarily clarify the link between gut dysbiosis and prostate cancer.

Results: First, we discovered that perturbing the gut microbiota by consuming broad-spectrum antibiotics in water promoted the growth of subcutaneous and orthotopic tumors in mice. Fecal microbiota transplantation could transmit the effect of antibiotic exposure on tumor growth. Then, 16S rRNA sequencing for mouse feces indicated that the relative abundance of Proteobacteria was significantly higher after antibiotic exposure. Meanwhile, intratumoral lipopolysaccharide (LPS) profoundly increased under the elevation of gut permeability. Both in vivo and in vitro experiments revealed that the NF-κB-IL6-STAT3 axis activated by intratumoral LPS facilitated prostate cancer proliferation and docetaxel chemoresistance. Finally, 16S rRNA sequencing of patients' fecal samples revealed that Proteobacteria was enriched in patients with metastatic prostate cancer and was positively correlated with plasma IL6 level, regional lymph node metastasis status, and distant metastasis status. The receiver operating characteristic (ROC) curves showed that the relative abundance of Proteobacteria had better performance than the prostate-specific antigen (PSA) level in predicting the probability of distant metastasis in prostate cancer (area under the ROC curve, 0.860; p < 0.001).

Conclusion: Collectively, this research demonstrated that gut dysbiosis, characterized by the enrichment of Proteobacteria due to antibiotic exposure, resulted in the elevation of gut permeability and intratumoral LPS, promoting the development of prostate cancer via the NF-κB-IL6-STAT3 axis in mice. Considering findings from human patients, Proteobacteria might act as an intestinal biomarker for progressive prostate cancer. Video Abstract.
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http://dx.doi.org/10.1186/s40168-022-01289-wDOI Listing
June 2022

VN and SeS embedded porous carbon-nanofiber film as a free-standing electrode for improved Li-SeS batteries.

Chem Commun (Camb) 2022 Jun 16. Epub 2022 Jun 16.

CAS Key Laboratory of Materials for Energy Conversions, Department of Materials Science and Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, University of Science and Technology of China, Anhui Hefei, 230026, China.

We design a vanadium nitride (VN) modified porous carbon nanofiber film as the host to load SeS as the cathode ([email protected]/CNFs) for improving Li storage capacity. The conductive porous carbon nanofibers can accommodate active SeS and release the volume change. The introduced VN nanoparticles can chemically anchor the intermediate species and improve the utilization of SeS. As a result, the [email protected]/CNFs cathode displays a superior electrochemical performance including a high reversible capacity of 806 mAh g at 0.2 C and good long-term cycling stability in Li-SeS batteries.
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http://dx.doi.org/10.1039/d2cc02218dDOI Listing
June 2022

Construction of a piezoelectric immunosensor for ultra-sensitive and highly selective detection of ribavirin in animal-derived foods.

Anal Methods 2022 Jun 15. Epub 2022 Jun 15.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.

In this work, a suppressive piezoelectric immunosensor based on the ultra-sensitive quartz crystal microbalance and highly specific immunosorbent assay was constructed for the detection of ribavirin for the first time. The carboxyl group was modified on the surface of the gold electrode by electropolymerization, and then the carboxyl group was combined with the amino-modified antigen, and then the corresponding antibody was able to bind to it according to the principle of immune recognition, which interlocked to cause the frequency change of the piezoelectric sensor. The preparation conditions of the sensor and the antibody concentration were optimized, and the performance of the sensor was evaluated. Under the optimal conditions, a wide linear range of 1-750 μg L, a low detection limit (IC) of 2.64 μg L, and a good sensitivity (IC) of 31.49 μg L were obtained. The sensor was used for the detection of ribavirin in chicken and milk samples, and the recovery rate ranged from 88.01 to 94.42%, which shows satisfactory consistency with the detection results of high performance liquid chromatography. It was suggested that the proposed piezoelectric immunosensor has good sensitivity and selectivity for ribavirin, and can be applied to the detection of actual food samples.
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http://dx.doi.org/10.1039/d2ay00771aDOI Listing
June 2022

Identification of the target protein of the metastatic colorectal cancer-specific aptamer W3 as a biomarker by aptamer-based target cells sorting and functional characterization.

Biosens Bioelectron 2022 Jun 3;213:114451. Epub 2022 Jun 3.

Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110122, PR China. Electronic address:

Metastasis is a leading cause of cancer-related deaths. Hence, the discovery of more reliable metastasis-related biomarkers is crucial to improve the survival rate of cancer patients. W3 is an aptamer previously produced by the subtractive cell-SELEX using metastatic colorectal cancer cells as target cells and non-metastatic cells as negative cells. In this study, we aimed to evaluate whether the target molecule of W3 can potentially act as a metastatic biomarker. First, we obtained two cell subpopulations with different expression levels of the target molecule by W3-based cell sorting. Subsequently, we demonstrated that W3 cells have a higher metastatic potential than W3 cells both in vitro and in vivo. Further, immunohistochemical analysis revealed that W3 target expression is positively associated with metastasis and poor prognosis of CRC patients. Using mass spectrometry (MS) combined with pull-down, we identified that Ephrin type-A receptor 2 (EphA2) is the target of W3. EphA2's potential as a metastatic predictor was demonstrated by capturing W3-positive circulating tumor cells from CRC patients using a W3 probe. Based on these results, we put forward a stratagem for cell-SELEX-based biomarker discovery: selecting an aptamer through subtractive cell-SELEX towards the phenotype of interest; evaluating the functional phenotype of the target molecule by aptamer-based target cell sorting and analysis of clinical samples; and identifying the aptamer's target molecule using MS and aptamer-based target enrichment. This stratagem not only shortens the time for the clinical application of aptamers but also enables a more targeted and efficient discovery of biomarkers.
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http://dx.doi.org/10.1016/j.bios.2022.114451DOI Listing
June 2022

Study on repair of abdominal wall defect rats with hernia mesh coated with chitosan-based photosensitive hydrogel.

Carbohydr Polym 2022 Sep 7;291:119577. Epub 2022 May 7.

Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology, Qingdao 266235, PR China. Electronic address:

Herein, hydroxypropyl chitosan azide (AZ-HPCTS) was synthesized and prepared as a hydrogel coating applied to a polypropylene mesh (PPM) through UV irradiation. This study confirmed the hypothesis that hydrogels with porous three-dimensional network structures exhibited excellent biocompatibility and biodegradability and adhered well to PPM. During the 180-day follow-up period, the AZ-HPCTS-coated PPM (AH-PPM) promoted wound healing by promoting the secretion of transforming growth factor-beta1 (TGF-beta1) in the acute reaction stage, which was reduced to a lower level at 30 d. The PPM exhibited a lower fibrin lysozyme activity based on the expression of tissue plasminogen activator (tPA) compared with that of AH-PPM (P < 0.05). The intraperitoneal adhesion score of AH-PPM decreased to 2.4 at 180 d in contrast with PPM (P < 0.01), which remained at a high level throughout the study. In conclusion, the AZ-HPCTS hydrogel is a potential coating for hernia patches that deserves further study in the biomaterial field.
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http://dx.doi.org/10.1016/j.carbpol.2022.119577DOI Listing
September 2022

Perspective on recent developments of nanomaterial based fluorescent sensors: Applications in safety and quality control of food and beverages.

J Food Drug Anal 2020 Dec 15;28(4):486-507. Epub 2020 Dec 15.

State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China.

As the highly toxic pollutants will seriously harm human health, it is particularly important to establish the analysis and detection technology of food pollutants. Compared with the traditional detection methods, fluorescent detection techniques based on nanomaterials trigger wide interesting because of reduced detection time, simple operation, high sensitivity and selectivity, and economic. In this review, the application of fluorescent sensors in food pollutants detection is presented. Firstly, conventional fluorescent nanomaterials including metal-based quantum dots, carbon dots, graphene quantum dots and metal nanoclusters were summarized, with emphasis on the photoluminescence mechanism. Then, the fluorescence sensors based on these nanomaterials for food pollutants detection were discussed, involving in the established methods, sensor mechanisms, sensitivity, selectivity, and practicability of fluorescence sensors. The selected analytes focus on five types of higher toxic food pollutants, including mycotoxins, foodborne pathogens, pesticide residues, antibiotic residues, and heavy metal ions. Finally, outlook on the future and potential development of fluorescence detection technology in the field of food science were proposed, including green synthesis and reusability of fluorescence probes, large-scale industrialization of sensors, nondestructive testing of samples and degradation of harmful substances.
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http://dx.doi.org/10.38212/2224-6614.1270DOI Listing
December 2020

Ni Nanocrystals Supported on Graphene Oxide: Antibacterial Agents for Synergistic Treatment of Bacterial Infections.

ACS Omega 2022 Jun 24;7(22):18339-18349. Epub 2022 May 24.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, PR China.

The effects of antibiotics on bacterial infections are gradually weakened, leading to the wide development of nanoparticle-based antibacterial agents with unique physical and chemical properties and antibacterial mechanisms different from antibiotics. In this study, we fabricated the uniform and stable graphene oxide (GO)/Ni colloidal nanocrystal cluster (NCNC) nanocomposite by electrostatic self-assembly and investigated its synergistic antibacterial activity against () and () in vitro. The GO/NCNC nanocomposite was shown to possess higher inhibition efficiency than a pure NCNC or GO suspension, with 99.5 and 100% inhibition against and at a 125 μg/mL concentration, respectively. Antibacterial mechanism analysis revealed that (i) NCNCs decorated on GO can further enhance the antibacterial properties of GO by binding and capturing bacteria, (ii) the leaching of Ni was detected during the interaction of GO/NCNCs and bacteria, resulting in a decrease in the number of bacteria, and (iii) the GO/NCNC nanocomposite can synergistically destroy the bacterial membrane through physical action and induce the reactive oxygen species generation, so as to further damage the cell membrane and affect ATPase, leakage of intercellular contents, and ultimately bacterial growth inhibition. Meanwhile, cell culture experiments demonstrated no adverse effect of GO/NCNCs on cell growth. These preliminary results indicate the high antibacterial efficiency of the GO/NCNC nanocomposite, suggesting the possibility to develop it into an effective antibacterial agent in the future against bacterial infections.
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http://dx.doi.org/10.1021/acsomega.2c00508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178720PMC
June 2022

Extrahepatic organs in the development of non-alcoholic fatty liver disease in liver transplant patients.

Hepatobiliary Surg Nutr 2022 Jun;11(3):400-411

Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background And Objective: Non-alcoholic fatty liver disease (NAFLD) is highly prevalent in patients who undergo liver transplantation (LT). Whereas there is huge data on NAFLD, little is known about NAFLD in LT. In this review, we aim to explore extrahepatic organs and their potential mechanisms in the development of NAFLD in LT patients and discuss current limitations in preclinical and clinical scenarios with suggestions for future study.

Methods: The following keywords, such as NAFLD, NASH, liver transplant, therapy, pathogenesis and biomarkers, were set for literature retrieval. The articles which were published articles in English till 25th June 2020 in PubMed database were included, and there is no limit for the study design type.

Key Content And Findings: Following LT, there are significant shifts in the microbiota and farnesoid X receptor may be a potential therapeutic target for NAFLD in LT settings. The roles of probiotics and diet on NALFD remain inconclusive in LT background. Nevertheless, the adipokines and cytokines disorder and local insulin resistance of adipose tissue may contribute to NAFLD process. Bariatric surgeries are promising in controlling de novo and recurrent NAFLD with significant reduction in abdominal adipose tissue, despite the optimal timing is inconclusive in LT cases. Furthermore, circumstantial evidence indicates that miRNA-33a may function as a mediator bridging sarcopenia and NAFLD of post-LT. β-Hydroxy-β-Methyl-Butyrate treatment could improve muscle status in graft recipients and shows protective potential for NAFLD in LT settings.

Conclusions: Gut, adipose tissue and muscle are intricately intertwined in promoting NAFLD in LT cases. Further animal studies are needed to deepen our understanding of mechanisms in multi-organ crosstalk. High quality clinical trials are warrant for making guidelines and developing management strategies on NAFLD after LT.
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http://dx.doi.org/10.21037/hbsn-20-568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186206PMC
June 2022

Expansion of CD3CD8PD1 T lymphocytes and TCR repertoire diversity predict clinical responses to adoptive cell therapy in advanced gastric cancer.

Am J Cancer Res 2022 15;12(5):2203-2215. Epub 2022 May 15.

Department of Medical Oncology, Beijing Key Laboratory for Therapeutic Cancer Vaccines, Capital Medical University Cancer Center, Beijing Shijitan Hospital, Capital Medical University Beijing 100038, China.

The adoptive cell therapy (ACT) and delivery of ex vivo activated cellular products, such as dendritic cells (DCs), NK cells, and T cells, have shown promise for the treatment of gastric cancer (GC). However, it is unknown which cells can improve patient survival. This study was focused on the antitumour activity of a subset of these cellular products and their relationships with clinical outcomes. Nineteen patients were enrolled at the Capital Medical University Cancer Center, Beijing Shijitan Hospital, from June 1, 2013, to May 30, 2016. CD8PD1 T-cell sorting was carried out using flow cytometry, and the T-cell receptor (TCR) repertoire during ex vivo expansion for 15 days was analyzed by next-generation sequencing. After 15 days of culture, the number of CD8 T cells had increased significantly, and the number of CD4 T cells had increased correspondingly. After ex vivo expansion, CD8 T cells exhibited significantly enhanced expression of PD-1, LAG-3, and TIM-3 but not 4-1BB. Survival analysis showed that patients with a pro/pre value of CD8PD-1 T cells >2.4 had significantly favorable overall survival (OS) (median OS time, 248 days versus 96 days, P=0.02) and progression-free survival (PFS) (median PFS time, 183 days vs. 77 days, P=0.002). The sorted CD8PD-1 T cells displayed enhanced antitumor activity and increased IFN-γ secretion after coculture with autologous tumor cell lines. TCR repertoire diversity was decreased after ex vivo expansion, which decreased the Shannon index and increased the clonality value. The prognosis of patients was significantly improved and was associated with the extent of CD8PD-1 T-cell expansion. In summary, this study showed that after ex vivo expansion for 15 days, CD8PD-1 T cells could be identified as tumor-reactive cells in patients treated for GC. Changing TCR species can predict the extent of CD3CD8PD1 T-cell growth and the effect of ACT treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185603PMC
May 2022

Distinct oxygen isotope fractionations driven by different electron donors during microbial nitrate reduction in lake sediments.

Environ Microbiol Rep 2022 Jun 12. Epub 2022 Jun 12.

Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing, China.

Microbial nitrate reduction can be driven by organic carbon oxidation, as well as by inorganic electron donors, such as reduced forms of sulfur and iron. An apparent inverse oxygen isotope fractionation effect was observed during nitrate reduction in sediment incubations from five sampling sites of a freshwater lake, Hongze Lake, China. Incubations with organic and inorganic electron donor additions were performed. Especially, the inverse oxygen isotope effect was intensified after glucose addition, whereas the incubations with sulfide and Fe showed normal fractionation factors. Nitrate reductase encoding genes, napA and narG, were analysed with metagenomics. Higher napA/narG ratios were associated with higher oxygen fractionation factors. The most abundant clade (59%) of NapA in the incubation with glucose was affiliated with Rhodocyclales. In contrast, it only accounted for 8%-9% of NapA in the incubations with sulfide and Fe . Differences in nitrate reductases might explain different oxygen isotope effects. Our findings also suggested that large variance of O-nitrate isotope fractionations might have to be considered in the interpretation of natural isotope records.
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http://dx.doi.org/10.1111/1758-2229.13101DOI Listing
June 2022

Fluorescence sensor based on molecularly imprinted polymers and core-shell upconversion [email protected] frameworks for detection of bovine serum albumin.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 6;279:121460. Epub 2022 Jun 6.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Medical College, Nankai University, Tianjin 300457, PR China. Electronic address:

A novel strategy for sensing protein was proposed through combining the high selectivity of molecular imprinting technology with the excellent upconversion fluorescence of upconversion nanoparticles (UCNPs) and high specific surface area of metal-organic frameworks (MOFs). Herein, the UCNPs acted as signal reporter and MOFs were introduced to increase the rate of mass transfer. The [email protected] as support material was prepared via a step-by-step method. The imprinted material-coated [email protected] ([email protected]@MIPs) were obtained by sol-gel technique. The results showed that as the increase of the template protein concentration, the fluorescence intensity of [email protected]@MIPs quenched gradually, and the imprinting factor was 2.90. The linear in the range of 1.00 to 8.00 μM, and the detection limit was 0.59 μM. Therefore, the novel optosensing material is very promising for future applications.
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http://dx.doi.org/10.1016/j.saa.2022.121460DOI Listing
October 2022

3,6'-Disinapoylsucrose alleviates the amyloid precursor protein and lipopolysaccharide induced cognitive dysfunction through upregulation of the TrkB/BDNF pathway.

J Asian Nat Prod Res 2022 Jun 7:1-16. Epub 2022 Jun 7.

Beijing Key Laboratory of Bioactive Substances and Functional Foods, Institute of Functional Food Science and Technology, Beijing Union University, Beijing 100023, China.

The aim of this study is to explore the effect and mechanism of 3,6'-disinapoylsucrose (DISS) on an Alzheimer's disease (AD) mice model induced by APPswe695 lentivirus (LV) and intraperitoneal injection of lipopolysaccharide (LPS). The results show that DISS improves cognitive ability, decreases the levels of IL-2, IL-6, IL-1β, and TNF-α, reduces the expression of NF-κB p65, and alleviates Aβ deposition and nerve cell damage. DISS can regulate tyrosine kinase B (TrkB)/brain-derived neurotrophic factor (BDNF) signaling in the hippocampus. In summary, DISS can significantly alleviate neuroinflammation, spatial learning and memory disorders in AD model mice.
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http://dx.doi.org/10.1080/10286020.2022.2069565DOI Listing
June 2022

A neuronal social trait space for first impressions in the human amygdala and hippocampus.

Mol Psychiatry 2022 Jun 8. Epub 2022 Jun 8.

Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, WV, 26506, USA.

People instantaneously evaluate faces with significant agreement on evaluations of social traits. However, the neural basis for such rapid spontaneous face evaluation remains largely unknown. Here, we recorded from 490 neurons in the human amygdala and hippocampus and found that the neuronal activity was associated with the geometry of a social trait space. We further investigated the temporal evolution and modulation on the social trait representation, and we employed encoding and decoding models to reveal the critical social traits for the trait space. We also recorded from another 938 neurons and replicated our findings using different social traits. Together, our results suggest that there exists a neuronal population code for a comprehensive social trait space in the human amygdala and hippocampus that underlies spontaneous first impressions. Changes in such neuronal social trait space may have implications for the abnormal processing of social information observed in some neurological and psychiatric disorders.
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http://dx.doi.org/10.1038/s41380-022-01583-xDOI Listing
June 2022

Expansion and Diversification of the 14-3-3 Gene Family in Camellia sinensis.

J Mol Evol 2022 Jun 4. Epub 2022 Jun 4.

School of Science, Qiongtai Normal University, Hainan, China.

14-3-3 proteins are signal moderators in sensing various stresses and play essential functions in plant growth and development. Although, 14-3-3 gene families have been identified and characterized in many plant species, its evolution has not been studied systematically. In this study, the plant 14-3-3 family was comprehensively analyzed from green algae to angiosperm. Our result indicated that plant 14-3-3 originated during the early evolutionary history of green algae and expanded in terricolous plants. Twenty-six 14-3-3 genes were identified in the tea genome. RNA-seq analysis showed that tea 14-3-3 genes display different expression patterns in different organs. Moreover, the expression of most tea 14-3-3 genes displayed variable expression patterns under different abiotic and biotic stresses. In conclusion, our results elucidate the evolutionary origin of plant 14-3-3 genes, and beneficial for understanding their biological functions and improving tea agricultural traits in the future.
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http://dx.doi.org/10.1007/s00239-022-10060-6DOI Listing
June 2022

Biomarker-based risk model to predict cardiovascular events in patients with acute coronary syndromes - Results from BIPass registry.

Lancet Reg Health West Pac 2022 Aug 30;25:100479. Epub 2022 May 30.

Department of Emergency and Chest Pain Center, Qilu Hospital, Shandong University, Jinan 250012, China.

Background: Risk models integrating new biomarkers to predict cardiovascular events in acute coronary syndromes (ACS) are lacking. Therefore, we evaluated the prognostic value of biomarkers in addition to clinical predictors and developed a biomarker-based risk model for major adverse cardiovascular events (MACE) within 12 months after hospital admission with ACS.

Methods: Patients ( = 4407) consecutively enrolled from November, 2017 to October, 2019 in three hospitals of a prospective Chinese registry (BIomarker-based Prognostic Assessment for Patients with Stable Angina and Acute Coronary Syndromes, BIPass) were designated as the risk model development cohort. Validation was performed in 1409 patients enrolled in two independent hospitals. Cox proportional hazards regression analysis was used to generate a risk prediction model and evaluate the incremental prognostic value of each biomarker.

Findings: Over 12 months, 196 patients experienced MACE (5.1%/year). Among twelve candidate biomarkers, N-terminal pro-B-type natriuretic peptide (NT-proBNP) measured at baseline showed the most prognostic capability independent of clinical predictors. The developed BIPass risk model included age, hypertension, previous myocardial infarction, stroke, Killip class, heart rate, and NT-proBNP. It displayed improved discrimination (C-statistic 0.79, 95% CI 0.73-0.85), calibration (GOF = 9.82,  = 0.28) and clinical decision curve in the validation cohort, outperforming the GRACE and TIMI risk scores. Cumulative rates for MACE demonstrated good separation in the BIPass predicted low, intermediate, and high-risk groups.

Interpretation: The BIPass risk model, integrating clinical variables and NT-proBNP, is useful for predicting 12-month MACE in ACS. It effectively identifies a gradient risk of cardiovascular events to aid personalized care.

Funding: National Key R&D Program of China (2017YFC0908700, 2020YFC0846600), National S&T Fundamental Resources Investigation Project (2018FY100600, 2018FY100602), Taishan Pandeng Scholar Program of Shandong Province (tspd20181220), Taishan Young Scholar Program of Shandong Province (tsqn20161065, tsqn201812129), Youth Top-Talent Project of National Ten Thousand Talents Plan and Qilu Young Scholar Program.
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http://dx.doi.org/10.1016/j.lanwpc.2022.100479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160492PMC
August 2022

Amelioration of TPA-induced skin inflammation by the leaf extract of Vernonia amygdalina involves ERK/STAT3 (Ser727) signaling inhibition.

Phytomedicine 2022 Jul 23;102:154194. Epub 2022 May 23.

Research and Development Centre for Natural Health Products, HKBU Institute for Research and Continuing Education, Shenzhen, China; Consun Chinese Medicines Research Centre for Renal Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. Electronic address:

Background: Uncontrolled inflammation causes health problems. Extracellular signal-regulated kinase (ERK) phosphorylates signal transducer and activator of transcription 3 (STAT3) at Ser727, resulting in inflammation. The leaf of Vernonia amygdalina (VA) is a medicinal herb for managing inflammation-associated diseases. Oral administration or topical application of VA leaf extract exerts anti-inflammatory effects in rat models. However, the anti-inflammatory mechanisms of the herb are not fully understood.

Purpose: In this study, we aimed to investigate the involvement of ERK/STAT3 (Ser727) signaling in the anti-inflammatory effects of an ethanolic extract of VA leaves.

Study Design And Methods: Extracts of VA leaves were prepared with different concentrations of ethanol. A LPS-stimulated RAW264.7 cell model was used for in vitro assays, and a TPA (12-O-tetradecanoylphorbol-13-acetate)-induced ear edema mouse model was employed for in vivo assays. The 95% ethanol extract of VA leaves (VAE) exerted the strongest inhibitory effect on nitric oxide (NO) production in LPS-stimulated macrophages; thus it was selected for use in this study. Hematoxylin and eosin (H&E) staining was used to examine pathological conditions of mouse ear tissues. Griess reagent was employed to examine NO generation in cell cultures. Immunoblotting and ELISA were used to examine protein levels, and RT-qPCR was employed to examine mRNA levels.

Results: Topical application of VAE ameliorated mouse ear edema induced by TPA. VAE suppressed the phosphorylation of ERK (Thr202/Tyr204) and STAT3 (Ser727); and decreased protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α) in the mouse ear tissues and in LPS-stimulated RAW 264.7 cells. VAE also inhibited NO production, and lowered mRNA levels of IL-6, IL-1β and TNF-α in the macrophages.

Conclusions: VAE ameliorates TPA-induced mouse ear edema. Suppression of ERK/STAT3 (Ser727) signaling is involved in VAE's anti-inflammatory effects. These novel data provide further pharmacological justifications for the medicinal use of VA in treating inflammation-associated diseases, and lay the groundwork for developing VAE into a new anti-inflammatory agent.
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http://dx.doi.org/10.1016/j.phymed.2022.154194DOI Listing
July 2022

RNA sequencing analysis between ruptured and un-ruptured brain AVM.

Chin Neurosurg J 2022 Jun 2;8(1):13. Epub 2022 Jun 2.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 119 South Fourth Ring Road West, Fengtai District, Beijing, 100071, China.

Background: A brain arteriovenous malformation (BAVM) is a tangle of abnormal blood vessels connecting the arteries and veins in the brain and is associated with a higher risk for intracerebral hemorrhage (ICH). RNA sequencing technology has been recently used to investigate the mechanism of diseases owing to its ability to identify the gene changes on a transcriptome-wide level. This study aims to gain insights into the potential mechanism involved in BAVM rupture.

Methods: Sixty-five BAVM nidus samples were collected, among which 28 were ruptured and 37 were un-ruptured. Then, next-generation RNA sequencing was performed on all of them to obtain differential expressed genes (DEGs) between the two groups. In addition, bioinformatics analysis was performed to evaluate the involved biological processes and pathways by GO and KEGG analysis. Finally, we performed a univariate Cox regression analysis to obtain the early rupture-prone DEGs.

Results: A total of 951 genes were differentially expressed between the ruptured and un-ruptured BAVM groups, of which 740 genes were upregulated and 211 genes were downregulated in ruptured BAVMs. Then, bioinformatics analysis showed the biological processes and pathways related to the inflammatory processes and extracellular matrix organization were significantly enriched. Meanwhile, some downregulated genes are involved in cell adhesion and genes participating in response to muscle activity and the terms of nervous system development. Finally, one hundred twenty-five genes, many were involved in inflammation, were correlated with the early rupture of BAVMs.

Conclusions: The upregulated genes in the ruptured BAVM group were involved in inflammatory processes and extracellular matrix organization. Some of the downregulated genes participated in cell adhesion and myofibril assembly, indicating the role of enhanced inflammation and reduced inflammation vessel strength in BAVMs rupture.
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http://dx.doi.org/10.1186/s41016-022-00282-4DOI Listing
June 2022

Efficacy and safety of Xuebijing injection for radiation pneumonitis: A meta-analysis.

PLoS One 2022 1;17(6):e0268819. Epub 2022 Jun 1.

Department of Oncology, Guang'an men Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Background: Currently, the treatment of radiation pneumonitis (RP) remains a clinical challenge. Although glucocorticoids are used for RP treatment, they have associated side effects. Xuebijing injection (XBJ) has been widely used for RP treatment in China, but so far no meta-analysis has evaluated its efficacy and safety.

Methods: PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure, WANFANG database, SinoMED, and China Science and Technology J Database were searched for randomized controlled trials related to XBJ in RP treatment. Two researchers independently conducted literature screening, data extraction, and risk of bias assessment. The outcomes were synthesized and analyzed using the Cochrane Review Manager (RevMan 5.3) software, and a forest plot generated.

Result: Eight articles met the eligibility criteria for further data extraction and meta-analysis. A total of 578 patients with RP participated in these studies, including 296 in the experimental group (XBJ+BT), and 282 in the control group (BT). The results of the meta-analysis revealed that compared to the BT group, XBJ+BT significantly increased the total effective rate (n = 578; RR = 1.45, 95% CI: 1.30 to 1.61, p<0.0001), and IL-10 expression (n = 296; MD = 17.62, 95% CI:13.95 to 21.29, p<0.00001), decreased interleukin-6 (IL-6) expression (n = 296; MD = -21.56, 95% CI:-27.37 to -15.76, p<0.00001), that of tumor necrosis factor alpha (n = 246; MD = -25.63, 95% CI:-30.77 to -20.50, p<0.00001), and that of C-reactive protein (n = 296; MD = -48.61, 95% CI:-56.49-40.73, p< 0.00001).

Conclusion: Based on our results, we do not recommend XBJ as an adjuvant treatment for RP. Further randomized controlled trials with rigorous design, strict implementation, and standard reporting are needed to further evaluate the efficacy and safety of XBJ for RP treatment.

Systematic Review Registration: INPLASY registration number: INPLASY2020120037.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268819PLOS
June 2022
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