Publications by authors named "Shuo Li"

944 Publications

MVSGAN: Spatial-aware Multi-view CMR Fusion for accurate 3D Left Ventricular Myocardium Segmentation.

IEEE J Biomed Health Inform 2021 Oct 26;PP. Epub 2021 Oct 26.

The accurate 3D left ventricular (LV) myocardium segmentation in short-axis (SAX) view of cardiac magnetic resonance (CMR) is challenged by the sparse spatial structure of CMR. The strategy of multi-view CMR fusion can provide fine-grained spatial structure for accurate segmentation. However, the large information misalignment & lack of dense 3D CMR as fusion target in multi-view CMR fusion, and the different spatial resolution between the fused cardiac model and the ground truth of segmentation in segmentation limit the strategy. In this study, we propose a multi-view spatial-aware adversarial network (MVSGAN). It studies the perception of fine-grained cardiac structure for accurate segmentation by the spatially consistent fusion of multi-view CMR. It consists of three modules: (1) A residual adversarial fusion (RAF) module takes inter-slices deep correlation and anatomical prior of multi-view CMR to refine the spatial structures by residual supplement and adversarial optimization. (2) A structural perception-aggregation (SPA) module establishes the spatial correlation between the spatially dense cardiac model and sparse segmentation label for accurate 3D CMR LV myocardium segmentation. (3) A joint training strategy utilizes the spatial dense SAX volume as explicit and implicit goals to jointly optimize the framework. The experiments are applied on a public dataset and a clinical dataset to evaluate the performance of MVSGAN. The average Dice and Jaccard score of LV myocardium segmentation obtained by MVSGAN are highest among seven existing state-of-the-art methods, which are up to 0.92 and 0.75. It is concluded that the spatial-aware multiview CMR fusion can provide meaningful spatial correlation for accurate 3D SAX LV myocardium segmentation.
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http://dx.doi.org/10.1109/JBHI.2021.3122581DOI Listing
October 2021

Experimental and Theoretical Study of the Effects of Rare Earth Elements on Growth and Chlorophyll of Alfalfa ( L.) Seedling.

Front Plant Sci 2021 8;12:731838. Epub 2021 Oct 8.

College of Grassland Agriculture, Northwest A&F University, Yangling, China.

Rare earth elements (REEs) of low concentration are usually beneficial to plant growth, while they are toxic at high concentrations. The effects of treatment with lanthanum (La) (10 and 20 μM), cerium (Ce) (10 and 20 μM), and terbium (Tb) (10 and 20 μM) on seedling growth of alfalfa ( L.), which is one of the most important perennial leguminous forages in the world, were studied. The results showed that all three REE treatments quickened the germination of seeds. The length of shoot under La (20 μM) treatment was significantly shortened ( < 0.05). In addition, treatment with La, Ce, and Tb had a "hormesis effect" on root length. There was a significant decrease in chlorophyll content on treatment with the three REEs, and the degree of decline was in the order of La < Ce < Tb, under the same concentration. experiments and quantum chemical calculations were further performed to explain why the treatments with REEs reduced the chlorophyll content. experiments showed that La, Ce, and Tb treatments reduced the absorbance of chlorophyll, and the decrease followed in the order of La > Ce > Tb. Quantum chemical calculations predicted that the decrease in absorption intensity was caused by the reactions between La, Ce, Tb, and chlorophyll, which formed lanthanides-chlorophyll; and there were five types of stable lanthanides-chlorophyll. In conclusion, the decrease in chlorophyll content on treatment with REEs was caused by the change in chlorophyll structure.
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http://dx.doi.org/10.3389/fpls.2021.731838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531810PMC
October 2021

Perioperative Use of Glucocorticoids and Intraoperative Hypotension May Affect the Incidence of Postoperative Infection in Patients with Gastric Cancer: A Retrospective Cohort Study.

Cancer Manag Res 2021 8;13:7723-7734. Epub 2021 Oct 8.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), The First Department of Gastrointestinal Surgery, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China.

Background: In patients undergoing surgical resection for gastric cancer, postoperative complications-in particular, postoperative infections-remain an important problem and can result in delayed recovery and increased postoperative mortality.

Objective: To investigate the association between perioperative anesthesia management and postoperative infectious complications in patients undergoing resection for gastric cancer.

Design: Retrospective cohort study.

Setting: A single-center study performed from April 1, 2015, to June 30, 2018, at Peking University Cancer Hospital.

Patients: Patients who underwent resection for gastric cancer.

Main Outcome Measures: Demographic information, perioperative data (including anesthesia-related data, surgery-related data, and cancer diagnosis), and information on postoperative recovery were recorded. The primary outcome was incidence of postoperative infection; the secondary outcome was length of hospital stay. The associations between perioperative factors and postoperative infectious complications were analyzed using multivariable logistic regression models and the classification tree method.

Results: A total of 880 patients were included in the study; of these, 111 (12.6%) had postoperative infectious complications during hospitalization, including 78 surgical site infections and 62 remote infections. After correction for confounding factors on logistic multivariable analysis, perioperative use of glucocorticoids was associated with a lower incidence of postoperative infection (hazard ratio 0.968, 95% confidence interval 0.939 to 0.997, =0.029), and intraoperative systolic blood pressure <90 mmHg for >10 min was associated with a higher incidence of postoperative infection (hazard ratio 2.112, 95% confidence interval 1.174 to 3.801, =0.013). In addition, older age, preoperative hypoproteinemia, and total gastrectomy were identified as independent predictors of postoperative infection.

Conclusion: For patients with gastric cancer, perioperative use of glucocorticoids and avoiding intraoperative hypotension may decrease the incidence of postoperative infectious complications.
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http://dx.doi.org/10.2147/CMAR.S333414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517416PMC
October 2021

Differentiation of Acquired Immune Deficiency Syndrome-Related Primary Central Nervous System Lymphoma from Cerebral Toxoplasmosis Using Susceptibility-Weighted Imaging and Contrast-Enhanced 3D-T1WI.

Int J Infect Dis 2021 Oct 17. Epub 2021 Oct 17.

Department of Radiology, Beijing Ditan Hospital, Capital Medical University. Electronic address:

Background: We aimed to investigate whether susceptibility-weighted imaging (SWI) and contrast-enhanced 3D-T1WI can differentiate Acquired Immune Deficiency Syndrome-Related Primary Central Nervous System Lymphoma(AR-PCNSL) from cerebral toxoplasmosis.

Methods: This was a prospective cohort study. 20 AIDS patients were divided into AR-PCNSL group (13 cases) and cerebral toxoplasmosis group (7 cases) based on pathology results. We analyzed the appearance of lesions on SWI and enhanced 3D T1WI and ROC curves in the diagnosis of AR-PCNSL and cerebral toxoplasmosis.

Results: Cerebral toxoplasmosis was more likely to show annular enhancement (p = 0.002), and complete smooth ring enhancement (p = 0.002). It was also more likely to present a complete, smooth low signal intensity rim (LSIR) (p = 0.002) and an incomplete, smooth LSIR (p = 0.019) on SWI. AR-PCNSL was more likely to present an incomplete, irregular LSIR (p = 0.000) and irregular central low signal intensity (CLSI) (p = 0.000) on SWI. The areas under the ROC curve of the SWI-ILSS grade and enhanced volume on 3D-T1WI were 0.872 and 0.862, respectively.

Conclusion: A higher SWI-ILSS grade and larger 3D-T1WI volume enhancement were diagnostic for AR-PCNSL. SWI and CE 3D-T1WI were useful in the differential diagnosis of AR-PCNSL and cerebral toxoplasmosis.
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http://dx.doi.org/10.1016/j.ijid.2021.10.023DOI Listing
October 2021

High microwave responsivity Co-BiFeO in synergistic activation of peroxydisulfate for high efficiency pollutants degradation and disinfection: Mechanism of enhanced electron transfer.

Chemosphere 2021 Oct 16:132558. Epub 2021 Oct 16.

Urban Water Resources Development and Northern National Engineering Research Center, Harbin, 150090, China; School of Environment, Harbin Institute of Technology, Harbin, 150090, China.

Cobalt doped BiFeO was used as a heterogeneous catalyst in microwave (MW) co-activation of peroxydisulfate (PDS) system for organic contaminant purification and disinfection simultaneously. Due to low charge-transfer resistance and fast electron migration, Co-BiFeO showed superior catalytic efficiencies for activation PDS to degrade over 92.0% of bisphenol A (BPA) with the initial concentrations ranging from 40 mg/L to 120 mg/L in 5.0 min. The non-radical oxidation pathway via electron transfer regime on the surface of Co-BiFeO was the dominant reactive species in the reaction system. Benefit from the energy transfer and cross-coupling reactions of microwave, the Co-BiFeO/MW/PDS system can generate abundant reactive sites to facilitate the formation of more surface-bonding complexes. Microwave energy can be absorbed by Co-BiFeO catalysts to promote activation of PDS and production of nanobubbles. The generated nanobubbles increase the temperature of the local solution to promote the reaction. The Co-BiFeO/MW/PDS system also exhibited excellent bactericidal capability for Escherichia coli (E.coli). The catalysts, oxidants and microwaves acted on E. coli to form physical, and oxidative pressure simultaneously, causing cell damaged and made bacterial death. This work provides prospects toward high-efficiency integration of contaminant purification and pathogenic microorganisms inactivation.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132558DOI Listing
October 2021

A simplified RT-PCR assay for the simultaneous detection of tomato chlorosis virus and tomato yellow leaf curl virus in tomato.

J Virol Methods 2021 Oct 11;299:114282. Epub 2021 Oct 11.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Lab of Food Quality and Safety of Jiangsu Province- State Key Laboratory Breeding Base, Nanjing, 210014, China. Electronic address:

Tomato chlorosis virus (ToCV), a species of single-stranded RNA virus belonging to the Crinivirus genus, and Tomato yellow leaf curl virus (TYLCV), a species of single-stranded circular DNA virus belonging to the Begomovirus genus, are two major emerging viruses transmitted by whiteflies and are causing huge losses to tomato production worldwide. To facilitate the simultaneous detection of both viruses in co-infected plants for disease control, a duplex reverse-transcription PCR assay was developed. The assay used three primers, a degenerate reverse primer targeting a conserved region of TYLCV and the RNA2 of ToCV, and two virus-specific forward primers targeting the minor coat protein gene of ToCV and the C3 gene of TYLCV, respectively, to amplify a 762-bp and a 338-bp fragment from ToCV and TYLCV, respectively, in a single reaction. The concentration of the primers, annealing temperature and amplification cycles used in the assay were optimized, and the sensitivity of the assay was assessed. Using this assay, 150 tomato leaf samples collected from the field during 2018 were tested. The results showed that both viruses could be detected simultaneously in co-infected field samples. The assay should benefit the rapid detection of these two viruses in tomato crops and would facilitate early warning of infections for the control of the two virus diseases.
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http://dx.doi.org/10.1016/j.jviromet.2021.114282DOI Listing
October 2021

Polymorphonuclear neutrophils promote endothelial apoptosis by enhancing adhesion upon stimulation by intermittent hypoxia.

Sleep Breath 2021 Oct 11. Epub 2021 Oct 11.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.

Purpose: This study explored the interactive effects between polymorphonuclear neutrophils (PMNs) and vascular endothelial cells under intermittent hypoxia (IH) and investigated the mechanisms underlying these effects.

Methods: Endothelial cells were co-cultured with PMNs isolated from rats exposed to normoxia or IH. The PMN apoptotic rate was determined using flow cytometry. Expression of apoptosis-related proteins in the endothelial cells were evaluated using Western blotting, and the levels of intercellular adhesion molecules in the co-culture supernatants were measured using enzyme-linked immunosorbent assay.

Results: The PMN apoptotic rate in the IH-exposed rat group was significantly lower than that of the normoxia control group. There was a positive relationship between the PMN apoptotic rate and IH exposure time. In endothelial cells co-cultured with PMNs isolated from IH-exposed rats, a significant increase in the protein expression levels of Bax, Bcl-2, and caspase-3 and a significant decrease in the Bcl-2/Bax ratio were observed. Furthermore, the intercellular cell adhesion molecule-1(ICAM-1) and E-select element (E-S) levels were elevated significantly in the co-cultured supernatants of endothelial cells and PMNs from IH-exposed rats compared to that from controls. The above IH-induced alterations were partially restored by tempol pretreatment.

Conclusions: The apoptotic rate was low in PMNs from IH-exposed rats, which consequently increased the apoptotic signals in endothelial cells in vitro. This may be associated with the increased levels of intercellular adhesion molecules. Further, tempol partially attenuates the PMN-mediated pro-apoptotic effects on endothelial cells under IH.
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http://dx.doi.org/10.1007/s11325-021-02503-zDOI Listing
October 2021

Advances in the study of emodin: an update on pharmacological properties and mechanistic basis.

Chin Med 2021 Oct 10;16(1):102. Epub 2021 Oct 10.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, Beijing, 100029, China.

Rhei Radix et Rhizoma, also known as rhubarb or Da Huang, has been widely used as a spice and as traditional herbal medicine for centuries, and is currently marketed in China as the principal herbs in various prescriptions, such as Da-Huang-Zhe-Chong pills and Da-Huang-Qing-Wei pills. Emodin, a major bioactive anthraquinone derivative extracted from rhubarb, represents multiple health benefits in the treatment of a host of diseases, such as immune-inflammatory abnormality, tumor progression, bacterial or viral infections, and metabolic syndrome. Emerging evidence has made great strides in clarifying the multi-targeting therapeutic mechanisms underlying the efficacious therapeutic potential of emodin, including anti-inflammatory, immunomodulatory, anti-fibrosis, anti-tumor, anti-viral, anti-bacterial, and anti-diabetic properties. This comprehensive review aims to provide an updated summary of recent developments on these pharmacological efficacies and molecular mechanisms of emodin, with a focus on the underlying molecular targets and signaling networks. We also reviewed recent attempts to improve the pharmacokinetic properties and biological activities of emodin by structural modification and novel material-based targeted delivery. In conclusion, emodin still has great potential to become promising therapeutic options to immune and inflammation abnormality, organ fibrosis, common malignancy, pathogenic bacteria or virus infections, and endocrine disease or disorder. Scientifically addressing concerns regarding the poor bioavailability and vague molecular targets would significantly contribute to the widespread acceptance of rhubarb not only as a dietary supplement in food flavorings and colorings but also as a health-promoting TCM in the coming years.
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http://dx.doi.org/10.1186/s13020-021-00509-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504117PMC
October 2021

New application of multimodal ultrasound imaging for identification of myofascial trigger points in the trapezius muscle.

Ann Palliat Med 2021 Sep;10(9):9784-9791

Department of Ultrasound Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: The study aimed to quantify the characteristics of trapezius myofascial trigger points (MTrPs) using shear wave elastography (SWE) and contrast-enhanced ultrasound (CEUS) and explore the application value of the new ultrasound techniques in identifying MTrPs.

Methods: Forty patients participated in this study. MTrPs in the trapezius muscle were determined by palpation, and SWE and CEUS were used to quantify the focal and adjacent areas. The elastic modulus values and CEUS parameters between the focal area of MTrPs and adjacent areas were evaluated and compared. Pathological biopsy was performed according to the above two methods, and the pathological tissues were observed by Masson staining, immunohistochemistry and electron microscope.

Results: The elastic modulus values were significantly higher for the focal area of MTrPs compared to those for adjacent areas (P<0.05). There were statistically significant differences in MTrP parameters, including peak intensity, mean transit time, and area between the focal and adjacent areas (P<0.05). Masson staining showed that there were inflammatory cell infiltration dominated by lymphocytes in the vascular wall. Electron microscopy showed a large number of fibroblast proliferation, lamellar collagen proliferation and lysosomal deposition; immunohistochemical results: the expression of CD3+, CD4+, CD8+, CD68+, mhc-1+, dys+, CD8 was more than that of CD20 (F=4.385, P=0.036).

Conclusions: Combined use of SWE and CEUS provides a new detection approach for quantitative identification of MTrPs in the trapezius muscle, which has high application value and is a method worthy of wider use in clinical practice.
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http://dx.doi.org/10.21037/apm-21-2103DOI Listing
September 2021

Interfacial Manipulation via In Situ Grown ZnSe Cultivator toward Highly Reversible Zn Metal Anodes.

Adv Mater 2021 Oct 7:e2105951. Epub 2021 Oct 7.

College of Energy, Soochow Institute for Energy and Materials Innovations (SIEMIS), Jiangsu Provincial Key Laboratory for Advanced Carbon Materials and Wearable Energy Technologies, Soochow University, Suzhou, 215006, P. R. China.

Zn metal anode has garnered growing scientific and industrial interest owing to its appropriate redox potential, low cost, and high safety. Nevertheless, the instability of Zn anode caused by dendrite formation, hydrogen evolution, and side reactions has greatly hampered its commercialization. Herein, an in situ grown ZnSe overlayer is crafted over one side of commercial Zn foil via chemical vapor deposition in a scalable manner, aiming to achieve optimized electrolyte/Zn interfaces with large-scale viability. Impressively, thus-derived ZnSe coating functions as a cultivator to guide oriented growth of Zn (002) plane at the infancy stage of stripping/plating cycles, thereby inhibiting the formation of Zn dendrites and the occurrence of side reactions. As a result, high cyclic stability (1530 h at 1.0 mA cm /1.0 mAh cm ; 172 h at 30.0 mA cm /10.0 mAh cm ) in symmetric cells is harvested. Meanwhile, when paired with V O based cathode, assembled full cell achieves an outstanding capacity (194.5 mAh g ) and elongated lifespan (a capacity retention of 84% after 1000 cycles) at 5.0 A g . The reversible Zn anode enabled by the interfacial manipulation strategy via ZnSe cultivator is anticipated to satisfy the demand of commercial use.
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http://dx.doi.org/10.1002/adma.202105951DOI Listing
October 2021

Do Engineered Nanomaterials Affect Immune Responses by Interacting With Gut Microbiota?

Front Immunol 2021 14;12:684605. Epub 2021 Sep 14.

Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Engineered nanomaterials (ENMs) have been widely exploited in several industrial domains as well as our daily life, raising concern over their potential adverse effects. While in general ENMs do not seem to have detrimental effects on immunity or induce severe inflammation, their indirect effects on immunity are less known. In particular, since the gut microbiota has been tightly associated with human health and immunity, it is possible that ingested ENMs could affect intestinal immunity indirectly by modulating the microbial community composition and functions. In this perspective, we provide a few pieces of evidence and discuss a possible link connecting ENM exposure, gut microbiota and host immune response. Some experimental works suggest that excessive exposure to ENMs could reshape the gut microbiota, thereby modulating the epithelium integrity and the inflammatory state in the intestine. Within such microenvironment, numerous microbiota-derived components, including but not limited to SCFAs and LPS, may serve as important effectors responsible of the ENM effect on intestinal immunity. Therefore, the gut microbiota is implicated as a crucial regulator of the intestinal immunity upon ENM exposure. This calls for including gut microbiota analysis within future work to assess ENM biocompatibility and immunosafety. This also calls for refinement of future studies that should be designed more elaborately and realistically to mimic the human exposure situation.
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http://dx.doi.org/10.3389/fimmu.2021.684605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476765PMC
October 2021

Regulation of fruit ripening by the brassinosteroid biosynthetic gene SlCYP90B3 via an ethylene-dependent pathway in tomato.

Hortic Res 2020 Oct 1;7(1):163. Epub 2020 Oct 1.

State Agricultural Ministry Laboratory of Horticultural Crop Growth and Development, Department of Horticulture, Zhejiang University, Hangzhou, 310058, China.

The essential role of ethylene in fruit ripening has been thoroughly studied. However, the involvement of brassinosteroids (BRs) in the regulation of fruit ripening and their relationship with the ethylene pathway are poorly understood. In the current study, we found that BRs were actively synthesized during tomato fruit ripening. We then generated transgenic lines overexpressing or silencing SlCYP90B3, which encodes a cytochrome P450 monooxygenase that catalyzes the rate-limiting step of BR synthesis. The expression level of SlCYP90B3 was positively related to the contents of bioactive BRs as well as the ripening process in tomato fruit, including enhanced softening and increased soluble sugar and flavor volatile contents. Both carotenoid accumulation and ethylene production were strongly correlated with the expression level of SlCYP90B3, corroborated by the altered expression of carotenoid biosynthetic genes as well as ethylene pathway genes in transgenic tomato fruits. However, the application of the ethylene perception inhibitor 1-methycyclopropene (1-MCP) abolished the promotion effect of SlCYP90B3 overexpression on carotenoid accumulation. Taken together, these results increase our understanding of the involvement of SlCYP90B3 in bioactive BR biosynthesis as well as fruit ripening in tomato, thus making SlCYP90B3 a target gene for improvement of visual, nutritional and flavor qualities of tomato fruits with no yield penalty.
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http://dx.doi.org/10.1038/s41438-020-00383-0DOI Listing
October 2020

Removal of F and organic matter from coking wastewater by coupling dosing FeCl and AlCl.

J Environ Sci (China) 2021 Dec 26;110:2-11. Epub 2021 Mar 26.

Key Laboratory of Drinking Water Science and Technology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100086, China. Electronic address:

Coagulation and precipitation is a widely applied method to remove F from wastewater. In this work, the effect of coagulation on the removal of F and organic matter from coking wastewater was studied using AlCl and FeCl as compound coagulants. The removal rates of F and organic matter under different coagulant doses and pH conditions were investigated. The results show that the highest removal rates of F by AlCl and FeCl are 94.4% and 25.4%, respectively; when the dosage is 10 mmol/L, the TOC removal rates of FeCl and AlCl reach 20.4% and 34.7%, respectively. Therefore, the removal rate of F by AlCl is higher than that of FeCl, but the removal rate of organic matter by FeCl is relatively higher. The addition of Ca can promote the removal of F, but the removal rate of organic matter decreases. In addition, by investigating the effects of different pH and Fe-Al ratio on the removal rate, the removal effect of adding FeCl and AlCl at the same time was discussed. The results show that the most suitable working condition for the removal of organic matter and F is that the pH is 6.5 and the molar ratio of Al/Fe is 8:2. Overall, the removal mechanism of F and organic matter in coking wastewater by FeCl and AlCl was explored in this study. The experimental results can provide reference for the advanced treatment of coking wastewater.
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http://dx.doi.org/10.1016/j.jes.2021.03.009DOI Listing
December 2021

Plastic response of L. root system traits and cold resistance to simulated rainfall events.

PeerJ 2021 9;9:e11962. Epub 2021 Sep 9.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Climate change (rainfall events and global warming) affects the survival of alfalfa ( L.) in winter. Appropriate water management can quickly reduce the mortality of alfalfa during winter. To determine how changes in water affect the cold resistance of alfalfa, we explored the root system traits under different rainfall events and the effects on cold resistance in three alfalfa cultivars. These were exposed to three simulated rainfall events (SRE) × two phases in a randomized complete block design with six replications. The three cultivars were WL168, WL353 and WL440, and the three SRE were irrigation once every second day (D), every four days (D) and every eight days (D). There were two phases: before cold acclimation and after cold acclimation. Our results demonstrated that a period of exposure to low temperature was required for alfalfa to achieve maximum cold resistance. The root system tended toward herringbone branching under D, compared with D and D, and demonstrated greater root biomass, crown diameter, root volume, average link length and topological index. Nevertheless, D had less lateral root length, root surface area, specific root length, root forks and fractal dimensions. Greater root biomass and topological index were beneficial to cold resistance in alfalfa, while more lateral roots and root forks inhibited its ability to survive winter. Alfalfa roots had higher proline, soluble sugar and starch content in D than in D and D. In contrast, there was lower malondialdehyde in D, indicating that alfalfa had better cold resistance following a longer irrigation interval before winter. After examining root biomass, root system traits and physiological indexes we concluded that WL168 exhibited stronger cold resistance. Our results contribute to greater understanding of root and cold stress, consequently providing references for selection of cultivars and field water management to improve cold resistance of alfalfa in the context of changes in rainfall patterns.
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http://dx.doi.org/10.7717/peerj.11962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435203PMC
September 2021

Dissected aorta segmentation using convolutional neural networks.

Comput Methods Programs Biomed 2021 Sep 15;211:106417. Epub 2021 Sep 15.

Laboratory of Imaging Science and Technology, Southeast University, Nanjing, China; School of Cyber Science and Engineering, Southeast University, Nanjing, China; Key Laboratory of Computer Network and Information Integration (Southeast University), Ministry of Education, Nanjing, China. Electronic address:

Background And Objective: Aortic dissection is a severe cardiovascular pathology in which an injury of the intimal layer of the aorta allows blood flowing into the aortic wall, forcing the wall layers apart. Such situation presents a high mortality rate and requires an in-depth understanding of the 3-D morphology of the dissected aorta to plan the right treatment. An accurate automatic segmentation algorithm is therefore needed.

Method: In this paper, we propose a deep-learning-based algorithm to segment dissected aorta on computed tomography angiography (CTA) images. The algorithm consists of two steps. Firstly, a 3-D convolutional neural network (CNN) is applied to divide the 3-D volume into two anatomical portions. Secondly, two 2-D CNNs based on pyramid scene parsing network (PSPnet) segment each specific portion separately. An edge extraction branch was added to the 2-D model to get higher segmentation accuracy on intimal flap area.

Results: The experiments conducted and the comparisons made show that the proposed solution performs well with an average dice index over 92%. The combination of 3-D and 2-D models improves the aorta segmentation accuracy compared to 3-D only models and the segmentation robustness compared to 2-D only models. The edge extraction branch improves the DICE index near aorta boundaries from 73.41% to 81.39%.

Conclusions: The proposed algorithm has satisfying performance for capturing the aorta structure while avoiding false positives on the intimal flaps.
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http://dx.doi.org/10.1016/j.cmpb.2021.106417DOI Listing
September 2021

Crystalline tetra-aniline with chloride interactions towards a biocompatible supercapacitor.

Mater Horiz 2021 Sep 29. Epub 2021 Sep 29.

College of Energy, Soochow Institute for Energy and Materials Innovations (SIEMIS), Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province, SUDA-BGI Collaborative Innovation Center, Soochow University, Suzhou 215006, P. R. China.

Recent advances in wearable and implantable electronics have increased the demand for biocompatible integrated energy storage systems. Conducting polymers, such as polyaniline (PANi), have been suggested as promising electrode materials for flexible biocompatible energy storage systems, based on their intrinsic structural flexibility and potential polymer chain compatibility with biological interfaces. However, due to structural disorder triggering insufficient electronic conductivity and moderate electrochemical stability, PANi still cannot fully satisfy the requirements for flexible and biocompatible energy storage systems. Herein, we report a biocompatible physiological electrolyte activated flexible supercapacitor encompassing crystalline tetra-aniline (c-TANi) as the active electrode material, which significantly enhances the specific capacitance and electrochemical cycling stability with chloride electrochemical interactions. The crystallization of TANi endows it with sufficient electronic conductivity (8.37 S cm) and a unique Cl dominated redox charge storage mechanism. Notably, a fully self-healable and biocompatible supercapacitor has been assembled by incorporating polyethylene glycol (PEG) with c-TANi as a self-healable electrode and a ferric-ion cross-linked sodium polyacrylate (Fe-PANa)/0.9 wt% NaCl as a gel electrolyte. The as-prepared device exhibits a remarkable capacitance retention even after multiple cut/healing cycles. With these attractive features, the c-TANi electrode presents a promising approach to meeting the power requirements for wearable or implantable electronics.
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http://dx.doi.org/10.1039/d1mh01081fDOI Listing
September 2021

[Reasearch on lipid metabolism of Plasmodium and antimalarial mechanism of artemisinin].

Zhongguo Zhong Yao Za Zhi 2021 Sep;46(18):4849-4864

Research Center of Artemisinin, China Academy of Chinese Medical Sciences Beijing 100700, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

As a unicellular organism, Plasmodium displays a panoply of lipid metabolism pathways that are seldom found together in a unicellular organism. These pathways mostly involve the Plasmodium-encoded enzymatic machinery and meet the requirements of membrane synthesis during the rapid cell growth and division throughout the life cycle. Different lipids have varied synthesis and meta-bolism pathways. For example, the major phospholipids are synthesized via CDP-diacylglycerol-dependent pathway in prokaryotes and de novo pathway in eukaryotes, and fatty acids are synthesized mainly via type Ⅱ fatty acid synthesis pathway. The available studies have demonstrated the impacts of artemisinin and its derivatives, the front-line compounds against malaria, on the lipid metabolism of Plasmodium. Therefore, this article reviewed the known lipid metabolism pathways and the effects of artemisinin and its derivatives on these pathways, aiming to deepen the understanding of lipid synthesis and metabolism in Plasmodium and provide a theoretical basis for the research on the mechanisms and drug resistance of artemisinin and other anti-malarial drugs.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210610.703DOI Listing
September 2021

Evaluation of Rabbits Liver Fibrosis Using Gd-DTPA-BMA of Dynamic Contrast-Enhanced Magnetic Resonance Imaging.

Evid Based Complement Alternat Med 2021 17;2021:2791142. Epub 2021 Sep 17.

Department of Radiology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning, China.

Objective: To evaluate the different pharmacokinetic parameters of the DCE-MRI method on diagnosing and staging of rabbits' liver fibrosis.

Methods: We had performed DCE-MRI for rabbits that had been divided into the experiment group and the control group. Then, rabbits' images were transferred to a work station to get three parameters such as , , and , which had been measured to calculate. After data were analyzed, ROC analyses were performed to assess the diagnostic performance of , , and to judge liver fibrosis.

Results: The distribution of the different liver fibrosis group was as follows: F1,  = 8; F2,  = 9; F3,  = 6; F4,  = 5. No fibrosis was deemed as F0,  = 6. is statistically significant ( < 0.05) for F0 and mild liver fibrosis stage, and the shows AUC of 0.814. Three parameters are statistically significant for F0 and advanced liver fibrosis stage ( and , < 0.01; , < 0.05), and the shows AUC of 0.924; the shows AUC of 0.909; the shows AUC of 0.848; and are statistically significant for mild and advanced liver fibrosis stages ( , < 0.01; , < 0.05), and the shows AUC of 0.840; the shows AUC of 0.765. Both and are negatively correlated with the liver fibrosis stage. is positively correlated with the liver fibrosis stage.

Conclusion: is shown to be the best DCE parameter to distinguish the fibrotic liver from the normal liver and mild and advanced fibrosis. On the contrary, is moderate and is worst. And is a good DCE parameter to differentiate mild fibrosis from the normal liver.
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http://dx.doi.org/10.1155/2021/2791142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463207PMC
September 2021

Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation.

Front Pharmacol 2021 8;12:754976. Epub 2021 Sep 8.

School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.

Chronic inflammation in response to persistent exogenous stimuli or damage results in liver fibrosis, which subsequently progresses into malignant liver diseases with high morbidity and mortality. Ferulic acid (FA) is a phenolic acid widely isolated from abundant plants and exhibits multiple biological activities including anti-oxidant, anti-inflammation and enhancement of immune responses. Adenosine monophosphate-activated protein kinase (AMPK) functions as a critical energy sensor and is regulated through the phosphorylation of liver kinases like LKB1 or dephosphorylation by protein tyrosine phosphatases (PTPs). However, the role of FA in carbon tetrachloride (CCl)-induced chronic inflammation and liver fibrosis and AMPK activation has not been elucidated. Here we reported that FA ameliorated CCl-induced inflammation and fibrotic liver damage in mice as indicated by reduced levels of serum liver function enzyme activities and decreased expression of genes and proteins associated with fibrogenesis. Additionally, FA inhibited hepatic oxidative stress, macrophage activation and HSC activation via AMPK phosphorylation in different liver cells. Mechanically, without the participation of LKB1, FA-induced anti-inflammatory and anti-fibrotic effects were abrogated by a specific AMPK inhibitor, compound C. Combining with the results of molecular docking, surface plasmon resonance and co-immunoprecipitation assays, we further demonstrated that FA directly bound to and inhibited PTP1B, an enzyme responsible for dephosphorylating key protein kinases, and eventually leading to the phosphorylation of AMPK. In summary, our results indicated that FA alleviated oxidative stress, hepatic inflammation and fibrotic response in livers through PTP1B-AMPK signaling pathways. Taken together, we provide novel insights into the potential of FA as a natural product-derived therapeutic agent for the treatment of fibrotic liver injury.
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http://dx.doi.org/10.3389/fphar.2021.754976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455917PMC
September 2021

Identification of autophagy-related genes in idiopathic pulmonary fibrosis using bioinformatics methods.

Chin Med J (Engl) 2021 Sep 22. Epub 2021 Sep 22.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.

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http://dx.doi.org/10.1097/CM9.0000000000001633DOI Listing
September 2021

Atypical asthma in children who present with isolated chest tightness: risk factors and clinical features.

J Asthma 2021 Sep 24:1-9. Epub 2021 Sep 24.

Allergy Department, Capital Institute of Pediatrics, Beijing, China.

Objective: Asthmatic children presenting with chest tightness as the only symptom have not been widely recognized. This study attempted to find risk factors, summarize clinical features and offer some suggestions for the diagnosis of this atypical asthma. We studied 94 children, aged 6 to 14 years, who complained only of chest tightness. Data from clinical manifestations and laboratory tests were analyzed. The atypical asthma group ( = 58) showed positive bronchial challenge tests, and symptoms either improved or resolved in response to the bronchodilator. The control group ( = 36) had negative results on the bronchial challenge, diurnal PEF, and BDR tests, and no response to asthma treatment with bronchodilator. Pollution, weather, recent house renovation, and air-conditioning use may be risk factors for children with atypical asthma. These children had more accompanying symptoms of rhinitis and rhinitis family history ( < 0.05), and a higher positive detection rate of inhaled allergens and multiple sensitizations. Parameters of the pulmonary function test were lower in the atypical asthma group than in the control group, and they also had higher FeNO values. If a cutoff value of improvement in FEV1 of BDR were set at 8.9%, sensitivity would be 48.2%, which is higher than a 12% cutoff. Environmental factors appeared to cause development of the isolated chest tightness symptom. Clinical history and laboratory tests could provide partial values for this diagnosis. In the absence of a bronchial challenge test, a margin of improvement in FEV1 of BDR set at 8.9% may be helpful.
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http://dx.doi.org/10.1080/02770903.2021.1980583DOI Listing
September 2021

Intramuscular Near-Infrared Spectroscopy for Muscle Flap Monitoring in a Porcine Model.

J Reconstr Microsurg 2021 Sep 22. Epub 2021 Sep 22.

Division of Plastic and Reconstructive Surgery, Department of Surgery, School of Medicine, Washington University, St. Louis, Missouri.

Background:  Current near-infrared spectroscopy (NIRS)-based systems for continuous flap monitoring are limited to flaps which carry a cutaneous paddle. As such, this useful and reliable technology has not previously been applicable to muscle-only free flaps where other modalities with substantial limitations continue to be utilized.

Methods:  We present the first NIRS probe which allows continuous monitoring of local tissue oxygen saturation (StO) directly within the substance of muscle tissue. This probe is flexible, subcentimeter in scale, waterproof, biocompatible, and is fitted with resorbable barbs which facilitate temporary autostabilization followed by easy atraumatic removal. This novel device was compared with a ViOptix T.Ox monitor in a porcine rectus abdominus myocutaneous flap model of arterial and venous occlusions. During these experiments, the T.Ox device was affixed to the skin paddle, while the novel probe was within the muscle component of the same flap.

Results:  The intramuscular NIRS device and skin-mounted ViOptix T.Ox devices produced very similar StO tracings throughout the vascular clamping events, with obvious and parallel changes occurring upon vascular clamping and release. The normalized cross-correlation at zero lag describing correspondence between the novel intramuscular NIRS and T.Ox devices was >0.99.

Conclusion:  This novel intramuscular NIRS probe offers continuous monitoring of oxygen saturation within muscle flaps. This experiment demonstrates the potential suitability of this intramuscular NIRS probe for the task of muscle-only free flap monitoring, where NIRS has not previously been applicable. Testing in the clinical environment is necessary to assess durability and reliability.
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http://dx.doi.org/10.1055/s-0041-1732361DOI Listing
September 2021

Sustainable Silk-Derived Multimode Carbon Dots.

Small 2021 Sep 21:e2103623. Epub 2021 Sep 21.

Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

Carbon dots (CDs) are widely studied for years due to their unique luminescent properties and potential applications in many fields. However, aggregation-caused quenching, monotonous emission modes, and unsustainable preparation impose restrictions on their performance and practical applications. Here, this work reports the facile synthesis of sustainable silk-derived multimode emitting CDs with dispersed-state fluorescence (DSF), aggregation-induced fluorescence (AIF), and aggregation-induced room temperature phosphorescence (AIRTP) through radiating sericin proteins in a household microwave oven (800 W, 2.5 min). The structure, luminescent properties, and the mechanism are investigated and discussed. The sericin-derived CDs have graphitized cores and heteroatom-cluster-rich surfaces. The DSF corresponds to the graphitized cores and the AIF origins from the aggregation-induced abundant orbital energy levels on the heteroatom-cluster-rich surfaces. The presence of abundant hydrogen bonds and small gap between the lowest singlet and triplet excited states induces AIRTP. Finally, based on the unique multimode emission of the prepared CDs, their applications in high-performance white-light-emitting diode, information encryption, anti-counterfeiting, and visual humidity sensors are demonstrated.
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http://dx.doi.org/10.1002/smll.202103623DOI Listing
September 2021

Liquid Alloying Na-K for Sodium Metal Anodes.

J Phys Chem Lett 2021 Sep 20;12(38):9321-9327. Epub 2021 Sep 20.

State Key Laboratory of Powder Metallurgy College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

The prospects of sodium (Na) metal batteries have been fatally plagued by interfacial Na dendrites, mainly affected by preferred nucleation on the metal anode and the steep gradient of Na ions in the electrolyte, leading to limited Coulombic efficiency and short lifespans. Herein, an electrochemically inert potassium-based Na-K alloy demonstrates a liquid alloying diffusion mechanism that enables dendrite-free Na anodes. The extremely small Na fluctuation and flexible Na-K bonds in the liquid alloy phase bring isotropic nucleation of Na upon electroplating/stripping, which is directly observed by optical imaging. Spontaneously, serving as (de)sodiation buffer with faster electron/mass transportation, the liquid inertia also provides attenuated concentration distribution of Na. Significantly, a record capacity retention of approximately 100% is rendered when coupled with NaV(PO) cathodes (. 2 mg cm) over 500 cycles at 10, advancing the possibility of using liquid alloy for stable metal anodes beyond Na storage systems.
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http://dx.doi.org/10.1021/acs.jpclett.1c02248DOI Listing
September 2021

Arachis hypogaea L. from Acid Soils of Nanyang (China) Is Frequently Associated with Bradyrhizobium guangdongense and Occasionally with Bradyrhizobium ottawaense or Three Bradyrhizobium Genospecies.

Microb Ecol 2021 Sep 15. Epub 2021 Sep 15.

Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647, New Zealand.

Henan Province is a major area of peanut production in China but the rhizobia nodulating the crop in this region have not been described. A collection of 217 strains of peanut rhizobia was obtained from six field sites across four soil types in Henan Province, North China, by using peanut as a trap host under glasshouse conditions. The 217 strains separated into 8 distinct types on PCR-RFLP analysis of their IGS sequences. Phylogenetic analysis of the 16S rRNA, recA, atpD, and glnII genes of 11 representative strains of the 8 IGS types identified Bradyrhizobium guangdongense, B. ottawaense and three novel Bradyrhizobium genospecies. Bradyrhizobium guangdongense was dominant, accounting for 75.0% of the total isolates across the field sites while B. ottawaense covered 5.1% and the three novel Bradyrhizobium genospecies 4.1 to 8.8% of the total. The symbiosis-related nodA and nifH gene sequences were not congruent with the core genes on phylogenetic analysis and separated into three groups, two of which were similar to sequences of Bradyrhizobium spp. isolated from peanut in south-east China and the third identical to that of B. yuanmingense isolated from Lespedeza cuneata in northern China. A canonical correlation analysis between the distribution of IGS genotypes and soil physicochemical characteristics and climatic factors indicated that the occurrence of IGS types/species was mainly associated with soil pH and available phosphorus.
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http://dx.doi.org/10.1007/s00248-021-01852-2DOI Listing
September 2021

Endovascular Treatment With and Without Intravenous Thrombolysis in Large Vessel Occlusions Stroke: A Systematic Review and Meta-Analysis.

Front Neurol 2021 30;12:697478. Epub 2021 Aug 30.

Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, Nanjing, China.

Previous studies have shown conflicting results about the benefits of pretreatment with intravenous thrombolysis before endovascular treatment (EVT) in patients with acute ischemic stroke (AIS) with large vessel occlusions (LVOs). This study aimed to investigate the clinical efficacy and safety of EVT alone vs. bridging therapy (BT) in patients with AIS with LVOs. A systematic review with meta-analysis of all available studies comparing clinical outcomes between BT and EVT alone was conducted by searching the National Center for Biotechnology Information/National Library of Medicine PubMed and Web of Science databases for relevant literature from database inception to October 20, 2020. A total of 93 studies enrolling 45,190 patients were included in the present analysis. In both unadjusted and adjusted analyses, BT was associated with a higher likelihood of 90-day good outcome (crude odds ratio [cOR] 1.361, 95% confidence interval [CI] 1.234-1.502 and adjusted OR [aOR] 1.369, 95% CI 1.217-1.540) and successful reperfusion (cOR 1.271, 95% CI 1.149-1.406 and aOR 1.267, 95% CI 1.095-1.465) and lower odds of 90-day mortality (cOR 0.619, 95% CI 0.560-0.684 and aOR 0.718, 95% CI 0.594-0.868) than EVT alone. The two groups did not differ in the occurrence of symptomatic intracranial hemorrhage (sICH) (cOR 1.062, 95% CI 0.915-1.232 and aOR 1.20, 95% CI 0.95-1.47), 24-h early recovery (cOR 1.306, 95% CI 0.906-1.881 and aOR 1.46, 95% CI 0.46-2.19), and number of thrombectomy device passes ≤ 2 (aOR 1.466, 95% CI 0.983-2.185) after sensitivity analyses and adjustment for publication bias. BT provides more benefits than EVT alone in terms of clinical functional outcomes without compromising safety in AIS patients with LVOs.
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http://dx.doi.org/10.3389/fneur.2021.697478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437100PMC
August 2021

Mesenchymal stem cell conditioned medium attenuates oxidative stress injury in hepatocytes partly by regulating the miR-486-5p/PIM1 axis and the TGF-β/Smad pathway.

Bioengineered 2021 12;12(1):6434-6447

Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

This study investigated the role of microRNA (miRNA) miR-486-5p in oxidative stress injury in hepatocytes under the treatment of mesenchymal stem cell conditioned medium (MSC-CM). The oxidative stress injury in hepatocytes (L02) was induced by HO. Human umbilical cord blood MSC-CM (UCB-MSC-CM) was prepared. The effects of UCB-MSC-CM on the proliferation, apoptosis, and inflammatory response in L02 cells were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry analysis, and enzyme-linked immunosorbent assay (ELISA). Subsequently, the target of miR-486-5p was predicted using bioinformatics analysis, and the possible signaling pathway addressed by miR-486-5p was explored using western blot. We found that miR-486-5p expression was elevated following oxidative stress injury and was reduced after UCB-MSC-CM treatment. UCB-MSC-CM protected L02 cells against HO-induced injury by downregulation of miR-486-5p. Proviral integration site for Moloney murine leukemia virus 1 (PIM1) was verified to be targeted by miR-486-5p. UCB-MSC-CM upregulated the expression of PIM1 reduced by HO in L02 cells. Additionally, silencing PIM1 attenuated the protective effects of miR-486-5p downregulation against oxidative stress injury. We further demonstrated that UCB-MSC-CM inhibited the TGF-β/Smad signaling in HOtreated L02 cells by the miR-486-5p/PIM1 axis. Overall, UCB-MSC-CM attenuates oxidative stress injury in hepatocytes by downregulating miR-486-5p and upregulating PIM1, which may be related to the inhibition of TGF-β/Smad pathway.
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http://dx.doi.org/10.1080/21655979.2021.1972196DOI Listing
December 2021

Hepsin regulates TGFβ signaling via fibronectin proteolysis.

EMBO Rep 2021 Sep 13:e52532. Epub 2021 Sep 13.

Research Programs Unit/Translational Cancer Medicine Research Program and Medicum, Faculty of Medicine, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.

Transforming growth factor-beta (TGFβ) is a multifunctional cytokine with a well-established role in mammary gland development and both oncogenic and tumor-suppressive functions. The extracellular matrix (ECM) indirectly regulates TGFβ activity by acting as a storage compartment of latent-TGFβ, but how TGFβ is released from the ECM via proteolytic mechanisms remains largely unknown. In this study, we demonstrate that hepsin, a type II transmembrane protease overexpressed in 70% of breast tumors, promotes canonical TGFβ signaling through the release of latent-TGFβ from the ECM storage compartment. Mammary glands in hepsin CRISPR knockout mice showed reduced TGFβ signaling and increased epithelial branching, accompanied by increased levels of fibronectin and latent-TGFβ1, while overexpression of hepsin in mammary tumors increased TGFβ signaling. Cell-free and cell-based experiments showed that hepsin is capable of direct proteolytic cleavage of fibronectin but not latent-TGFβ and, importantly, that the ability of hepsin to activate TGFβ signaling is dependent on fibronectin. Altogether, this study demonstrates a role for hepsin as a regulator of the TGFβ pathway in the mammary gland via a novel mechanism involving proteolytic downmodulation of fibronectin.
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http://dx.doi.org/10.15252/embr.202152532DOI Listing
September 2021

Inflammatory Factors as Potential Markers of Early Neurological Deterioration in Acute Ischemic Stroke Patients Receiving Endovascular Therapy - The AISRNA Study.

J Inflamm Res 2021 2;14:4399-4407. Epub 2021 Sep 2.

Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, People's Republic of China.

Background And Purpose: This study aimed to explore several peripheral blood-based markers related to the inflammatory response in a total of 210 patients with acute ischemic stroke (AIS) caused by large artery occlusion in the anterior circulation who received endovascular therapy (EVT) from an observational study of clinical significance of circulating non-coding RNA in acute ischemic stroke (AISRNA).

Methods: We collected baseline characteristics of 210 AIS patients participating in an observational acute stroke cohort: the AISRNA study. The following inflammatory factors were measured in these participants: interleukin-2 [IL-2], IL-4, IL-6, IL-10, tumor necrosis factor-α [TNF-α], and interferon-γ [IFN-γ]. The National Institute of Health Stroke Scale score increase of ≥4 within 24 hours after EVT defined as early neurological deterioration (END).

Results: Compared with patients without END, patients with END had a higher incidence of atrial fibrillation (=0.012), and also had higher levels of IL-6 and IL-10 (<0.01). Furthermore, we found that the area under the curves (AUCs) of IL-6 and IL-10 for predicting END were 0.768 (0.697-0.829), and 0.647 (0.570-0.719), respectively. Adjusting for age, sex, and atrial fibrillation, the odds ratios (ORs; 95% confidence interval) for incident END for IL-6 and IL-10 were 1.98 (1.05-6.69) and 1.18 (1.04-1.33), respectively. Additionally, we found significant changes over time in the expression levels of IL-4, IL-6, and IL-10 in patients with END compared with patients without END (<0.05).

Conclusion: IL-6 and IL-10 levels at admission may be potential markers of END after EVT, and the time course of IL-4, IL-6, and IL-10 is correlated with stroke progression. Further larger studies are needed to confirm the current findings.

Trial Registration: ClinicalTrials.gov NCT04175691. Registered November 21, 2019, https://www.clinicaltrials.gov/ct2/show/NCT04175691.
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http://dx.doi.org/10.2147/JIR.S317147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421252PMC
September 2021
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