Publications by authors named "Shuo Chen"

910 Publications

Association of long-term exposure to PM in workplace with fasting plasma glucose among asymptomatic adults: A multicenter study in North China.

Environ Int 2022 Jun 16;166:107353. Epub 2022 Jun 16.

School of Medicine, Nankai University, Tianjin, China. Electronic address:

Background: The impacts of long-term high exposure to PM in workplace on glucose metabolism in asymptomatic working adults (AWAs) have rarely been explored.

Objectives: To assess the relationship between long-term exposure to workplace PM and glucose metabolism in asymptomatic general working adults in heavily polluted regions.

Methods: We used the baseline data of the asymptomatic working participants from the Beijing-Tianjin-Hebei Medical Examination Cohort, which recruited adults undergoing medical examinations. A machine learning-based spatial-temporal model was used to estimate daily average PM concentrations in the participants' workplaces. We assessed the association of long-term PM concentrations (three years prior to the interview) and fasting plasma glucose (FPG) using generalized linear mixed-effects models (GLMM) with inclusion of potential confounders. Stratified analyses by sex, age, BMI and smoking status, and two pollutant models were further performed.

Results: A total of 37,619 individuals were interviewed and 28,865 were included in the analyses. The mean FPG was 5.20 (0.96) mmol/L, and the estimated three-year average concentration of PM exposure was 69.51 (6.92) μg/m. We detected a significant association of long-term exposure to workplace PM and FPG, a 10 µg/m increase in the long-term workplace PM exposure was associated with 0.075 (95%CI: 0.050-0.100) mmol/L elevated FPG and 25% (OR = 1.25, 95%CI: 1.05-1.50) elevated odds of abnormal fasting glucose metabolism with control of the potential confounding. The detected association between workplace PM and FPG metabolism remained significant in males, individuals aged > 44 years, overweight and/or obese people, both smokers and non-smokers, and when NO, SO, O, and CO were included in the model.

Conclusions: Long-term exposure to workplace PM was associated with elevated FPG and/or odds of abnormal glucose metabolism among AWAs. Male, middle-aged, overweight and/or obese AWAs were more susceptible to workplace PM regardless of smoking status.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2022.107353DOI Listing
June 2022

Cu Release from Polylactic Acid Coating on Titanium Reduces Bone Implant-Related Infection.

J Funct Biomater 2022 Jun 10;13(2). Epub 2022 Jun 10.

Sichuan Provincial Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

Implant-related infection (IRI) is a major problem in orthopedics. Copper (Cu) is an essential trace element with strong bactericidal activity and, thus, presents potential for reducing IRI. The present study explored a straightforward strategy for releasing Cu from titanium (Ti) implants, and we conducted a preliminary study to assess the feasibility of this approach in clinical translation. Polylactic acid (PLA) coatings containing different concentrations of copper ions were prepared on Ti discs. The antibacterial activity and biocompatibility of the copper ion-incorporated Ti implants were evaluated using Staphylococcus aureus (), bone marrow mesenchymal stem cells (BMSCs) and animal models. In vitro, the coatings produced burst release of Cu in 12 h, and inhibited growth in a dose-dependent manner. The coatings prepared from PLA solutions containing 0.5 or 1.0 mg/mL reduced the viability and osteogenic differentiation of BMSCs, but these effects were negated after the coatings were immersed in culture medium for 6 h. Four weeks after implantation, the Cu-free K-wires challenged with had persistent infection and inferior fracture healing to the other three groups, while Cu-coated wires had no evidence of infection. Furthermore, the Cu-coated wires placed in rabbits without challenge showed superior fracture healing to the other three groups. These results suggest that PLA coatings containing Cu may be an effective design for reducing IRI without adversely affecting adjacent bone healing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jfb13020078DOI Listing
June 2022

Aberrant expression of GSTM5 in lung adenocarcinoma is associated with DNA hypermethylation and poor prognosis.

BMC Cancer 2022 Jun 21;22(1):685. Epub 2022 Jun 21.

Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University-Daqing, No. 39 Xinyang Street, High-tech Zone, Daqing, 163319, Heilongjiang Province, China.

Background: Glutathione-S transferases (GSTs) comprise a series of critical enzymes involved in detoxification of endogenous or xenobiotic compounds. Among several GSTs, Glutathione S-transferases mu (GSTM) has been implicated in a number of cancer types. However, the prognostic value and potential functions of the GSTM family genes have not been investigated in lung adenocarcinoma (LUAD).

Methods: We examined the expression of GSTM5 in LUAD and identified associations among GSTM5 expression, clinicopathological features, survival data from the Cancer Genome Atlas (TCGA). The correlation between GSTM5 DNA methylation and its expression was analyzed using the MEXPRESS tool and UCSC Xena browser. The methylation status of GSTM5 in the promoter region in lung cancer cells was measured by methylation-specific PCR (MSP). After 5-aza-2'-deoxycytidine treatment of lung cancer cells, expression of GSTM5, cell proliferation and migration were assessed by RT-PCR, CCK-8 and transwell assays, respectively.

Results: The results showed that GSTM5 was abnormally down-regulated in LUAD patients' tissues, and patients with low GSTM5 expression level had significantly shorter OS. Cox regression analyses revealed that GSTM5 was associated with overall survival (OS) of LUAD patients, which expression was an independent prognostic indicator in terms of OS (hazard ratio: 0.848; 95% CI: 0.762-0.945; P = 0.003). In addition, we found the promoter region of GSTM5 was hypermethylated in the tumor tissue compared with adjacent normal tissues, and the average methylation level of GSTM5 were moderately correlated with its expression. Moreover, methylation-specific PCR also showed that the GSTM5 gene promoter was hypermethylated in lung cancer cells, and treatment with 5-Aza-CdR can restore the gene expression and inhibit cell proliferation and migration. Finally, Gene Set Enrichment Analysis (GSEA) revealed that low GSTM5 expression was significantly related to DNA repair pathways.

Conclusion: Our data demonstrate that low GSTM5 expression and its high DNA methylation status may act as a novel putative molecular target gene for LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-022-09711-0DOI Listing
June 2022

Patterns of Sexually Transmitted Co-infections and Associated Factors Among Men Who Have Sex With Men: A Cross-Sectional Study in Shenyang, China.

Front Public Health 2022 31;10:842644. Epub 2022 May 31.

National Health Commission Key Laboratory of AIDS Immunology, National Clinical Research Center for Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.

Background: Men who have sex with men (MSM) are disproportionately affected by sexually transmitted infections (STIs). We sought to describe patterns of sexually transmitted co-infections and explore factors associated with increased acquisition of STIs among MSM.

Methods: We enrolled MSM in Shenyang, China, between July and December 2020 to test for four STIs, including human papillomavirus (HPV), (CT), (NG), and (TP). Data regarding demographic and behavioral characteristics of participants were collected through a self-administered digital questionnaire. We adopted the ordinal logistic regression model to identify factors associated with acquiring more STIs.

Results: Overall, 177 participants with completed test results for all four STIs were analyzed. These participants had a median age of 29.0 (interquartile range: 23.0-38.0) years. The prevalence of STI co-infections was 23.7% [42/177; 95% confidence interval (CI), 17.8%-30.8%], among which HPV/CT (47.1%) and HPV/CT/NG (50.0%) co-infection were the predominant types among participants with dual and multiple infections, respectively. Participants who had a higher educational background [adjusted odds ratio (aOR), 0.46; 95% CI, 0.24-0.85; = 0.014] and had a history of STIs (aOR, 2.53; 95% CI, 1.24-5.18; = 0.011) were positively associated with acquiring more STIs.

Conclusions: MSM in Shenyang suffer a substantial burden of sexually transmitted co-infections. An optimized multi-STI integration strategy targeting prevention, surveillance, screening, and treatment is warranted to reduce the prevalence of sexually transmitted co-infections, especially in less-educated MSM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.842644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193278PMC
June 2022

Remote clinical research operations during COVID-19: lessons learned and recommendations.

Sleep 2022 Jun 14. Epub 2022 Jun 14.

Sleep Disorders Center, Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/sleep/zsac124DOI Listing
June 2022

Frontal white matter association with sleep quality and the role of stress.

J Sleep Res 2022 Jun 14:e13669. Epub 2022 Jun 14.

Department of Psychiatry, Maryland Psychiatric Research Center, School of Medicine, University of Maryland, Baltimore, Maryland, USA.

An important measure of brain health is the integrity of white matter connectivity structures that link brain regions. Studies have found an association between poorer sleep quality and decreased white matter integrity. Stress is among the strongest predictors of sleep quality. This study aimed to evaluate the association between sleep quality and white matter and to test if the relationship persisted after accounting for stress. White matter microstructures were measured by diffusion tensor imaging in a population of Old Order Amish/Mennonite (N = 240). Sleep quality was determined by the Pittsburgh Sleep Quality Index. Current stress levels were measured by the perceived stress scale. Exposure to lifetime stress was measured by the lifetime stressor inventory. Microstructures of four white matter tracts: left and right anterior limbs of internal capsule, left anterior corona radiata, and genu of corpus callosum were significantly correlated with sleep quality (all p ≤ 0.001). The current stress level was a significant predictor of sleep quality (p ≤ 0.001) while lifetime stress was not. PSQI remained significantly associated with white matter integrity in these frontal tracts (all p < 0.01) after accounting for current stress and lifetime stress, while current and lifetime stress were not significant predictors of white matter in any of the four models. Sleep quality did not have any substantial mediation role between stress and white matter integrity. Sleep quality was significantly associated with several frontal white matter tracts that connect brain structures important for sleep regulation regardless of current or past stress levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jsr.13669DOI Listing
June 2022

Roles of the RhoA-ROCK Signaling Pathway in the Endothelial HS Production and Vasodilation in Rat Cerebral Arteries.

ACS Omega 2022 Jun 20;7(22):18498-18508. Epub 2022 May 20.

Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, Anhui, China.

Cerebral endothelial HS protects against cerebral ischemia-reperfusion injury through vasodilation, but its cerebral vasodilation mechanism and regulation of production are poorly understood. The RhoA-ROCK pathway plays important roles in vascular function. In this study, the roles of this pathway in the endothelial HS production and vasodilation in rat cerebral arteries were investigated. Acetylcholine significantly increased HS-generating enzyme cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) protein expressions and HS production in rat cerebrovascular endothelial cells (ECs), but the increases were markedly decreased by the M receptor blocker atropine or the CSE inhibitor dl-propargylglycine. Pretreatment with dl-propargylglycine or the 3-MST inhibitor l-aspartic acid markedly reduced the acetylcholine-increased HS; CSE protein expression and HS levels in the ECs were obviously attenuated by the RhoA agonist U but increased by the RhoA inhibitor C3 transferase. U also reduced 3-MST protein expression; Acetylcholine markedly inhibited RhoA protein expression and activity, but the inhibition was obviously reversed by atropine, dl-propargylglycine, and l-aspartic acid, respectively; Acetylcholine-induced endothelium-dependent vasodilation in rat cerebral basilar artery was significantly attenuated by pretreatment with dl-propargylglycine or l-aspartic acid or RhoA inhibitor CCG-1423 or ROCK inhibitor KD025, and was further decreased by co-pretreatment with dl-propargylglycine (or l-aspartic acid) and CCG-1423 (or KD025); NaHS significantly relaxed rat cerebral basilar artery vascular smooth muscle cells and inhibited ROCK activities, phosphorylated myosin light chain (MLC) protein expression, and KCl-increased [Ca], but these relaxation and inhibitions were markedly attenuated by pretreatment with C3 transferase or ROCK inhibitor Y27632. Our results demonstrated that endothelial HS production is promoted by activation of the M receptor but inhibited by the RhoA-ROCK pathway in rat cerebral arteries; the endothelial HS induces cerebral vasodilation by inhibiting this pathway to reduce phosphorylation of MLC and [Ca] in vascular smooth muscle cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.2c00996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178624PMC
June 2022

Fluvirucins B-B, new antifungal macrolactams from a marine-derived sp. MYH522.

RSC Adv 2022 May 20;12(24):15479-15485. Epub 2022 May 20.

School of Pharmacy, Naval Medical University 325 Guo-He Road Shanghai 200433 People's Republic of China

Marine rare actinomycetes are an important source of secondary metabolites. From a marine-derived actinomycete sp. MYH522, four new macrolactams, fluvirucins B-B, together with known fluvirucin B were isolated. Their structures were determined based on comprehensive analysis of HRESIMS and NMR spectroscopic data as well as by comparing C NMR resonances and optical rotation values with those for related congeners. Fluvirucins are characterized by a 14-membered macrolactam attached by an aminosugar moiety. The discovery of fluvirucins B-B enriched the -acetylated derivatives of fluvirucins. The diverse alkyl substituents at C-2 and C-6 implied substrate promiscuity in fluvirucin polyketide biosynthesis. These compounds didn't exhibit any antibacterial or antifungal activities when used alone, which suggested the importance of the free amino group in the antimicrobial activity of fluvirucins. However, fluvirucins B, B, and B showed synergistic antifungal activity with fluconazole against fluconazole-resistant isolates of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2ra01701fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121787PMC
May 2022

Inflow Angle Impacts Morphology, Hemodynamics, and Inflammation of Side-wall Intracranial Aneurysms.

J Magn Reson Imaging 2022 Jun 2. Epub 2022 Jun 2.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, Tsinghua University, Beijing, China.

Background: Aneurysm inflow angle has been shown to be associated with hemodynamic changes by computational fluid dynamics. However, these studies were based on single aneurysm model and were limited to side-wall aneurysms.

Purpose: To investigate the association between inflow angle and morphology, hemodynamic, and inflammation of intracranial side-wall and bifurcation aneurysms.

Study Type: Prospective.

Population: A total of 62 patients (aged 58.34 ± 12.39, 44 female) with 59 unruptured side-wall aneurysms and 17 unruptured bifurcation aneurysms were included.

Field Strength/sequence: A 3.0 T; 3D fast field echo sequence (TOF-MRA); free-breathing, 3D radio-frequency-spoiled, multi-shot turbo field echo sequence (4D-flow MRI); 3D black-blood T1-weighted volumetric turbo spin echo acquisition sequence (T -VISTA) ASSESSMENT: Two neuroradiologists assessed the inflow angle and size for intracranial aneurysms in 3D space with TOF-MRA images. The average and maximum inflow velocity (V , V ), blood flow (Flow , Flow ), and average wall shear stress (WSS ) for aneurysms were assessed from 4D-flow MRI in regions of interest drawn by two neuroradiologists. The aneurysmal wall enhancement (AWE) grades between precontrast and postcontrast T -VISTA images were evaluated by three neuroradiologists.

Statistical Tests: Kruskal-Wallis H test, χ test, Pearson's correlation coefficient, scatter plots and regression lines, multivariate logistic regression analysis (partial correlation r) were performed. A P < 0.05 was considered statistically significant.

Results: The WSS (0.52 ± 0.34 vs. 0.27 ± 0.22) and AWE grades (1.38 ± 1.04 vs. 2.02 ± 0.68) between the two inflow angle subgroups of side-wall aneurysms were significantly different. The aneurysm size (r  = 0.31), WSS (r  = -0.45), and AWE grades (r  = 0.45) were significantly correlated with inflow angle in side-wall aneurysms. While in bifurcation aneurysms, there were no significant associations between inflow angle and size (P = 0.901), V (P = 0.699), V (P = 0.482), Flow (P = 0.550), Flow (P = 0.689), WSS (P = 0.573), and AWE grades (P = 0.872).

Data Conclusion: A larger aneurysm size, a lower WSS and a higher AWE grade were correlated with a larger inflow angle in side-wall aneurysms.

Evidence Level: 3 TECHNICAL EFFICACY: Stage 2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmri.28234DOI Listing
June 2022

Blood flow and perfusion lateralization in border zone infarct using 4D flow and arterial spin labeling.

Neuroradiology 2022 May 30. Epub 2022 May 30.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

Purpose: Hemodynamics may play an important role in border zone infarct (BZI), but macroscopic and microscopic hemodynamics of BZI still remain unclear. This study aims to investigate arterial flow and tissue perfusion differences between BZI and non-BZI in patients with unilateral middle cerebral artery (MCA) territory infarcts.

Methods: Subacute ischemic stroke patients with unilateral infarcts at MCA territory were included. Imaging protocols included 4D flow, ASL (arterial spin labeling), and routine clinical brain MRI scan. A total of 56 patients (56.1 ± 11.9 years, 39 male) were included and divided as BZI (n = 26) and non-BZI (n = 30). BZI was further subdivided as cortical BZI (CBZI, n = 9), internal BZI (IBZI, n = 11), and mixed BZI (n = 6). Average blood flow (Flow), regional average cerebral blood flow (CBF) were compared between infarct and contralateral sides to test hemodynamic lateralization. Flow-index and CBF-index (infarct sides/contralateral sides) were compared between groups and subgroups.

Results: Flow and CBF showed significant lateralization in both BZI and non-BZI as well as CBZI and IBZI. Flow-index (0.51 ± 0.37 vs. 0.87 ± 0.36, p < 0.01) and CBF-index (0.70 ± 0.21 vs. 0.90 ± 0.19, p < 0.01) were significantly different between BZI and non-BZI but were not significantly different between CBZI and IBZI.

Conclusion: In summary, hemodynamic lateralization can occur in subacute stroke patients with BZI and non-BZI and the one that occurs in BZI tends to be more severe in view of arterial flow and tissue perfusion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00234-022-02967-0DOI Listing
May 2022

Canonical Wnt/β-catenin signaling has positive effects on osteogenesis, but can have negative effects on cementogenesis.

J Periodontol 2022 Jun 1. Epub 2022 Jun 1.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Background: To date, therapeutic approaches for cementum regeneration are limited and outcomes remain unpredictable. A significant barrier to improve therapies for cementum regeneration is that the cementocyte and its intracellular signal transduction mechanisms remain poorly understood. This study aims to elucidate the regulatory mechanism of Wnt pathway in cementogenesis.

Methods: The effects of canonical Wnt signaling were compared in vitro using immortalized murine cementocyte cell line IDG-CM6 and osteocyte cell line IDG-SW3 by qRT-PCR, Western blot, confocal microscopy, alkaline phosphatase (ALP) assay and Alizarin red S staining. In vivo, histological changes of cementum and bone formation were examined in transgenic mice in which constitutive activation of β-catenin is driven by Dmp1 promoter.

Results: Expression of components of the Wnt/β-catenin pathway were much greater in the IDG-SW3 cells compared to the IDG-CM6 cells resulting in much lower expression of Sost/sclerostin in the IDG-SW3 cells. In the IDG-CM6 cells, low dose Wnt3a (20 ng/ml) had a modest effect while high dose (200 ng/ml) inhibited runt-related transcription factor 2 (Runx2), osterix (Osx), ALP and osteopontin (OPN) in contrast to the IDG-SW3 cells where high dose Wnt3a dramatically increased mRNA expression of these same markers. However, high Wnt3a significantly increased mRNA for components of Wnt/β-catenin signaling pathway in both IDG-CM6 and IDG-SW3 cells. In vivo, constitutive activation of β-catenin in the Dmp1-lineage cells in mice leads to bone hyperplasia and cementum hypoplasia.

Conclusion(s): These findings indicate that Wnt signaling has distinct and different effects on the regulation of long bone as compared to cementum. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/JPER.21-0599DOI Listing
June 2022

Pharmacological effects of polydatin in the treatment of metabolic diseases: A review.

Phytomedicine 2022 Jul 13;102:154161. Epub 2022 May 13.

Institution of Shenzhen Hospital, Guangzhou University of Chinese Medicine (Futian), Shenzhen, 518000, China. Electronic address:

Background: Metabolic diseases (MDs), a series of chronic disorders, severely decreases the quality of life for patients but also cause a heavy economic burden. Emerging evidence suggests that Polydatin (PD), an important glucoside of resveratrol, is widely distributed in many plants and has shown good therapeutic potential in metabolic diseases.

Purpose: To review the PD discovered before 2021 and their potential to treat metabolic diseases. The activities against diabetes, Obesity, atherosclerosis, NAFLD, NASH, hyperlipidemia, and gout with special emphasis on pharmacology, pharmacokinetics, mechanisms of action, possible roles in current medicine, and future perspectives are discussed.

Methods: A comprehensive search of published literature was conducted to locate original publications pertaining to polydatin and MDs through the end of 2021 using MEDLINE, Elsevier, Springer, PubMed, Scholar, and CNKI databases. The main inquiry used was for the presence of the following keywords in various combinations in the abstracts: 'Polydatin', 'Metabolic diseases', 'Pharmacology', 'Toxicology', 'Pharmacokinetics', 'Diabetes', 'Obesity', 'Atherosclerosis', 'Non-alcoholic fatty liver disease', 'Non-alcoholic steatohepatitis', 'Hyperlipidemia', and 'Gout'.

Results: The search yielded 987 articles, of which 33 articles were included in this review. Studies have revealed that PD can promote insulin secretion, alleviate insulin resistance, regulate glucose and lipid metabolism, reduce liver lipid deposition, inhibit inflammation, oxidative stress, and decrease uric acid deposition in preclinical experiments. The underlying mechanisms of PD in treatment MDs may be attributed to the regulation of multiple signaling pathways, including. NF-κB, AGEs/RAGE, MAPK/ERK, AMPK/LDLR, IRS1/PI3K/AKT, LKB1/AMPK, PPARβ-NO, SIRT1-PGC-1α-SOD2, PKC, etc., The pharmacokinetic profiles of PD provide valuable information on therapeutic efficacy in treating metabolic diseases.

Conclusion: This review summarizes the available reports and evidence which support the use of PD as a potential candidate in the treatment of MDs and provides an overview of the modulatory effects of PD in metabolic diseases and cell signaling pathways, which may have important implications in its future clinical use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2022.154161DOI Listing
July 2022

Advancements in Skin Delivery of Natural Bioactive Products for Wound Management: A Brief Review of Two Decades.

Pharmaceutics 2022 May 17;14(5). Epub 2022 May 17.

School of Pharmacy, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1010, New Zealand.

Application of modern delivery techniques to natural bioactive products improves their permeability, bioavailability, and therapeutic efficacy. Many natural products have desirable biological properties applicable to wound healing but are limited by their inability to cross the stratum corneum to access the wound. Over the past two decades, modern systems such as microneedles, lipid-based vesicles, hydrogels, composite dressings, and responsive formulations have been applied to natural products such as curcumin or aloe vera to improve their delivery and efficacy. This article reviews which natural products and techniques have been formulated together in the past two decades and the success of these applications for wound healing. Many cultures prefer natural-product-based traditional therapies which are often cheaper and more available than their synthetic counterparts. Improving natural products' effect can provide novel wound-healing therapies for those who trust traditional compounds over synthetic drugs to reduce medical inequalities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics14051072DOI Listing
May 2022

Visible light driven antibiotics degradation using S-scheme BiWO/CoInS heterojunction: Mechanism, degradation pathways and toxicity assessment.

Chemosphere 2022 May 24;303(Pt 1):135113. Epub 2022 May 24.

School of Resources, Environment and Materials, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, MOE Key Laboratory of New Processing Technology for Non-ferrous Metals and Materials, Nanning, 530004, China. Electronic address:

S-scheme heterojunction photocatalysts with strong redox ability and excellent photocatalytic activity are highly desired for photocatalytic degradation of pollutants. Herein, S-scheme BiWO/CoInS heterojunctions were synthesized using hydrothermal method. The photo-induced carriers transfer mechanism of the S-scheme BiWO/CoInS heterojunction was clarified by band structure analysis, ultraviolet photoelectron spectrometer (UPS), electron spin resonance (ESR) and radical trapping experiments. Significant enhance of light absortion, and more efficient carriers separation were observed from the BiWO/CoInS with CoInS nanoclusters growing on the surface of petal-like BiWO nanosheets. TC degradation efficiency of 90% was achieved by BiWO/CoInS (15:1) within 3 h of irradiation, and ·Oand ·OH radicals were dominated contributors. Possible decomposition pathways of TC were proposed, and ECOSAR analysis showed that most of the intermediates exhibited lower ecotoxicity than TC. This work provides reference on the constructing ternary-metal-sulfides-based S-scheme heterojunctions for improving photocatalytic performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2022.135113DOI Listing
May 2022

Ultrasound Radiomics-Guided Iliac Fascia Block on Postoperative Cognitive Dysfunction in Elderly Patients Undergoing Hip Surgery.

Comput Math Methods Med 2022 17;2022:2492667. Epub 2022 May 17.

Faculty of Anesthesiology, Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University, Suzhou, Jiangsu 215153, China.

Objective: Elderly patients with hip surgery are prone to postoperative cognitive dysfunction (POCD), leading to health management difficulties. This study is aimed at investigating the effect of ultrasound radiomics-guided iliac fascia block on POCD.

Methods: A total of 67 cases of patients who had undergone hip joint surgery were divided into a training set ( = 47) and a validation set (radiomics-guided group, = 20). The patients were intervened with ultrasound radiomics-guided iliac fascia block, and the maximum relevance minimum redundancy sifts out the image omics features obtained from 2D ultrasound images of patients. Another 20 patients undergone general anesthesia served as control. The incidence of POCD, the total amount of fentanyl, the visual analogue score (VAS) at different time points, and the levels of CRP and NSE in plasma were compared between the two groups.

Results: The AUC on the training and validation sets were higher than 0.940. The incidence of POCD in the radiomics-guided and general anesthesia group was 5% and 30%, respectively ( = 0.037). Compared with the general anesthesia group, the dosage of fentanyl in the radiomics-guided was lower, the VAS score at 6 h, 1 d, and 2 d after operation was smaller, and the levels of CRP and NSE were lower (all < 0.05).

Conclusions: For elderly patients with hip surgery, the ultrasound radiomics-guided iliac fascia block can reduce the incidence of POCD and improve the effect of nerve block.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/2492667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129949PMC
May 2022

Association Between Habitual Night Sleep Duration and Predicted 10-Year Cardiovascular Risk by Sex Among Young and Middle-Aged Adults.

Nat Sci Sleep 2022 11;14:911-926. Epub 2022 May 11.

School of Medicine, Nankai University, Tianjin, People's Republic of China.

Purpose: We hypothesize the association between sleep duration and cardiovascular disease (CVD) risk varies with age category; however, evidence for the relationship between sleep duration and CVD risk among young and middle-aged adults remains scarce. This research aims to assess the association between night sleep duration and cardiovascular risk by sex among young and middle-aged Chinese adults.

Patients And Methods: We used the baseline data of a cohort of adults for physical examination by stratified cluster sampling. The Framingham risk score and the Pittsburgh Sleep Quality Index were used to measure CVD risk and sleep duration, respectively. Demographic characteristics, lifestyle factors, height, weight, total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) were collected. We performed multiple logistic regressions to examine the association between night sleep duration and the predicted cardiovascular risk.

Results: We included 27,547 participants aged 18-64 years free of CVD, cerebral stroke, and not taking lipid-lowering agents. Overall, 12.7%, and 20.4% were at medium and high predicted CVD risk, respectively; 11.9% and 12.3% reported short and long sleep, respectively. Short sleep was independently associated with 23% (95% CI: 1.08-1.40) increased odds of medium-to-high CVD risk and 26% (95% CI: 1.11-1.45) increased odds of high CVD risk among females. Whereas long sleep was independently associated with 17% (95% CI: 0.71-0.98) decreased odds of medium-to-high CVD risk among males.

Conclusion: Among young and middle-aged adults, long sleep was associated with decreased odds of CVD risk in males, whereas short sleep was associated with increased odds of cardiovascular risk in females.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/NSS.S359611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109730PMC
May 2022

Glycated Hemoglobin and Risk of Arterial Stiffness in a Chinese Han Population: A Longitudinal Study.

Front Endocrinol (Lausanne) 2022 29;13:854875. Epub 2022 Apr 29.

School of Public Health, Capital Medical University, Beijing, China.

Background And Aims: Glycated hemoglobin (HbA1c) associates with the risk of arterial stiffness, and such association can be found between fasting blood glucose (FBG), postprandial blood glucose (PBG), triglyceride-glucose index (TyG index), and arterial stiffness. However, the results were inconsistent, longitudinal studies were sparse, and comparison of these glycemic parameters was less conducted. We aimed to explore the longitudinal relationship between HbA1c and arterial stiffness and compare the effect of the parameters.

Methods: Data were collected from 2011 to 2019 in Beijing Health Management Cohort (BHMC) study. Cox proportional hazard models were fitted to investigate the association between the parameters and arterial stiffness. A generalized estimation equation (GEE) analysis was conducted to investigate the effect of repeated measurements of glycemic parameters. A receiver operating characteristic (ROC) analysis was performed to compare the predictive value of glycemic parameters for arterial stiffness.

Results: Among 3,048 subjects, 591 were diagnosed as arterial stiffness during the follow-up. The adjusted hazard ratio (HR) [95% confidence interval (CI)] for arterial stiffness of the highest quartile group of HbA1c was 1.63 (1.22-2.18), which was higher than those of FBG, PBG, and TyG index. The nonlinear association of arterial stiffness with HbA1c and PBG was proved. The robust results of the sensitivity analysis were obtained.

Conclusions: HbA1c is an important risk factor of arterial stiffness compared with PBG, FBG, and TyG index, and has a strong predictive ability for arterial stiffness among non-diabetics and the general population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.854875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098962PMC
May 2022

Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells.

Pflugers Arch 2022 Jul 14;474(7):665-680. Epub 2022 May 14.

Department of Biopharmaceutical Sciences, School of Pharmacy, Harbin Medical University at Daqing, No. 39 Xinyang Rd, High-tech District, Daqing, 163319, Heilongjiang Province, China.

Mammalian expression systems, particularly the human embryonic kidney (HEK-293) cells, combined with electrophysiological studies, have greatly benefited our understanding of the function, characteristic, and regulation of various ion channels. It was previously assumed that the existence of endogenous ion channels in native HEK-293 cells could be negligible. Still, more and more ion channels are gradually reported in native HEK-293 cells, which should draw our attention. In this regard, we summarize the different ion channels that are endogenously expressed in HEK-293 cells, including voltage-gated Na channels, Ca channels, K channels, Cl channels, nonselective cation channels, TRP channels, acid-sensitive ion channels, and Piezo channels, which may complicate the recording of the heterogeneously expressed ion channels to a certain degree. We noted that the expression patterns and channel profiles varied with different studies, which may be due to the distinct originality of the cells, cell culture conditions, passage numbers, and different recording protocols. Therefore, a better knowledge of endogenous ion channels may help minimize potential problems in characterizing heterologously expressed ion channels. Based on this, it is recommended that HEK-293 cells from unknown sources should be examined before transfection for the characterization of their functional profile, especially when the expression level of exogenous ion channels does not overwhelm the endogenous ion channels largely, or the current amplitude is not significantly higher than the native currents.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00424-022-02700-zDOI Listing
July 2022

Ultrafast water permeation through nanochannels with a densely fluorous interior surface.

Science 2022 05 12;376(6594):738-743. Epub 2022 May 12.

Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Ultrafast water permeation in aquaporins is promoted by their hydrophobic interior surface. Polytetrafluoroethylene has a dense fluorine surface, leading to its strong water repellence. We report a series of fluorous oligoamide nanorings with interior diameters ranging from 0.9 to 1.9 nanometers. These nanorings undergo supramolecular polymerization in phospholipid bilayer membranes to form fluorous nanochannels, the interior walls of which are densely covered with fluorine atoms. The nanochannel with the smallest diameter exhibits a water permeation flux that is two orders of magnitude greater than those of aquaporins and carbon nanotubes. The proposed nanochannel exhibits negligible chloride ion (Cl) permeability caused by a powerful electrostatic barrier provided by the electrostatically negative fluorous interior surface. Thus, this nanochannel is expected to show nearly perfect salt reflectance for desalination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.abd0966DOI Listing
May 2022

Early assessment of chemotherapeutic response in hepatocellular carcinoma based on serum surface-enhanced Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 29;278:121314. Epub 2022 Apr 29.

College of Medicine and Biological Information Engineering, Northeastern University, Shenyang 110167, China; Key Laboratory of Intelligent Computing in Medical Image, Ministry of Education, China. Electronic address:

In clinical practice, the transcatheter arterial chemoembolization (TACE) has been widely accepted as the first option for non-surgical hepatocellular carcinoma (HCC) treatment. However, patients with HCC often suffer from poor response to TACE therapy. This can be prevented if the chemotherapeutic response can be early and accurately assessed, which is essential to guide timely and rational management. In this study, the serum SERS technique was for the first time investigated as a potential prognostic tool for early assessment of HCC chemotherapeutic response. According to the SERS spectral analysis results, it is newly found that not only the absolute circulating nucleic acids and collagen levels in pre-therapeutic serum but also the changes in circulating nucleic acids and amino acids between pre-therapeutic and post-therapeutic serum are expected to be potential serum markers for HCC prognosis. By further applying chemometrics methods to establish prognostic models, excellent prognostic accuracies were achieved within only 3 days after TACE therapy. Thus, the proposed method is expected to provide guidance on timely and rational management of HCC to improve its survival rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2022.121314DOI Listing
October 2022

Ginsenoside Rb1 Improves Metabolic Disorder in High-Fat Diet-Induced Obese Mice Associated With Modulation of Gut Microbiota.

Front Microbiol 2022 19;13:826487. Epub 2022 Apr 19.

State Key Laboratory of Genetic Engineering, Department of Microbiology and Immunology, School of Life Sciences, Fudan University, Shanghai, China.

Gut microbiota plays an important role in metabolic homeostasis. Previous studies demonstrated that ginsenoside Rb1 might improve obesity-induced metabolic disorders through regulating glucose and lipid metabolism in the liver and adipose tissues. Due to low bioavailability and enrichment in the intestinal tract of Rb1, we hypothesized that modulation of the gut microbiota might account for its pharmacological effects as well. Here, we show that oral administration of Rb1 significantly decreased serum LDL-c, TG, insulin, and insulin resistance index (HOMA-IR) in mice with a high-fat diet (HFD). Dynamic profiling of the gut microbiota showed that this metabolic improvement was accompanied by restoring of relative abundance of some key bacterial genera. In addition, the free fatty acids profiles in feces were significantly different between the HFD-fed mice with or without Rb1. The content of eight long-chain fatty acids (LCFAs) was significantly increased in mice with Rb1, which was positively correlated with the increase of and , and negatively correlated with the decrease of and . Among these eight increased LCFAs, eicosapentaenoic acid (EPA), octadecenoic acids, and myristic acid were positively correlated with metabolic improvement. Furthermore, the colonic expression of the () gene was significantly upregulated after Rb1 treatment, in response to a notable increase of LCFA in feces. These findings suggested that Rb1 likely modulated the gut microbiota and intestinal free fatty acids profiles, which should be beneficial for the improvement of metabolic disorders in HFD-fed mice. This study provides a novel mechanism of Rb1 for the treatment of metabolic disorders induced by obesity, which may provide a therapeutic avenue for the development of new nutraceutical-based remedies for treating metabolic diseases, such as hyperlipidemia, insulin resistance, and type 2 diabetes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2022.826487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062662PMC
April 2022

Gut-derived lipopolysaccharide promotes alcoholic hepatosteatosis and subsequent hepatocellular carcinoma by stimulating neutrophil extracellular traps through TLR4.

Clin Mol Hepatol 2022 May 4. Epub 2022 May 4.

Department of General Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.

Background: Binge drinking leads to many disorders, including alcoholic hepatosteatosis, which is characterized by intrahepatic neutrophil infiltration and which increases the risk of hepatocellular carcinoma (HCC). Molecular mechanisms may involve the migration of bacterial metabolites from the gut to the liver and activation of neutrophil extracellular traps (NETs).

Methods: Serum from both binge drinking and alcohol-avoiding patients was analyzed. Mouse models of chronical plus binge alcohol induced hepatosteatosis and HCC models were used.

Results: A marker of NETs formation, lipopolysaccharide, was significantly higher in alcoholic hepatosteatosis and HCC patients and mice than in controls. Intrahepatic inflammation markers and HCC-related cytokines were decreased in mice with reduced NET formation due to neutrophil elastase (NE) deletion, and liver-related symptoms of alcohol were also alleviated in NE KO mice. Removal of intestinal bacteria with antibiotics led to decreases in markers of NETs formation and inflammatory cytokines upon chronic alcohol consumption, and genesis of alcoholic hepatosteatosis and HCC was also attenuated. These functions were restored upon supplementation with the bacterial product lipopolysaccharide (LPS). When mice lacking TLR4 received chronic alcohol feeding, intrahepatic markers of NETs formation decreased, and hepatosteatosis and HCC were alleviated.

Conclusion: Formation of NETs following LPS stimulation of TLR4 upon chronic alcohol usage leads to increased alcoholic steatosis and subsequent HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3350/cmh.2022.0039DOI Listing
May 2022

Saturated multi-delay renal arterial spin labeling technique for simultaneous perfusion and T quantification in kidneys.

Magn Reson Med 2022 May 4. Epub 2022 May 4.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing, China.

Purpose: To propose a free-breathing simultaneous multi-delay arterial spin labeling (ASL) and T mapping technique with a stepwise kinetic model for renal assessment in a single 4-min scan at 3 T.

Methods: The proposed saturated multi-delay renal arterial spin labeling (SAMURAI) sequence used flow-sensitive alternating inversion recovery (FAIR) preparation, followed by acquisition of 9 images with Look-Locker spoiled gradient recalled echo (SPGR). Pre-saturation at the imaging slice was used to achieve saturation-based T mapping. A 4-step 2-compartment kinetic model was proposed to characterize water transition through artery- and tissue-compartment. The impact of the Look-Locker sampling scheme on the ASL signal was corrected in this model. T estimation with dictionary searching method and perfusion quantification based on the proposed kinetic model fitting were conducted after groupwise registration of the acquired images. The feasibility and repeatability of SAMURAI were validated in healthy subjects (n = 11) and patients with different renal diseases (n = 4).

Results: The proposed SAMURAI technique can provide accurate T map with strong correlation (R  = 0.98) with inversion recovery spin echo (IR-SE) on phantom. SAMURAI provided equally reliable whole kidney and cortical ASL and T quantification results compared with multi-TI FAIR (intraclass correlation coefficient [ICC], 0.880-0.958) and IR-SPGR (ICC, 0.875-0.912), respectively. Low renal blood flow and increased T were detected by SAMURAI in the affected kidneys of the patients. SAMURAI had excellent scan-rescan repeatability (ICC, 0.905-0.992) and significantly reduced scan time (4 min 6 s vs. 45 min for 9 TIs) compared to multi-TI FAIR.

Conclusion: The proposed SAMURAI technique is feasible and repeatable for simultaneously quantifying T and perfusion of kidneys with high time-efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mrm.29268DOI Listing
May 2022

Integrated regulation of chondrogenic differentiation in mesenchymal stem cells and differentiation of cancer cells.

Cancer Cell Int 2022 Apr 29;22(1):169. Epub 2022 Apr 29.

Department of Pathology, Tianjin Union Medical Center, Tianjin, 300071, People's Republic of China.

Chondrogenesis is the formation of chondrocytes and cartilage tissues and starts with mesenchymal stem cell (MSC) recruitment and migration, condensation of progenitors, chondrocyte differentiation, and maturation. The chondrogenic differentiation of MSCs depends on co-regulation of many exogenous and endogenous factors including specific microenvironmental signals, non-coding RNAs, physical factors existed in culture condition, etc. Cancer stem cells (CSCs) exhibit self-renewal capacity, pluripotency and cellular plasticity, which have the potential to differentiate into post-mitotic and benign cells. Accumulating evidence has shown that CSCs can be induced to differentiate into various benign cells including adipocytes, fibrocytes, osteoblast, and so on. Retinoic acid has been widely used in the treatment of acute promyelocytic leukemia. Previous study confirmed that polyploid giant cancer cells, a type of cancer stem-like cells, could differentiate into adipocytes, osteocytes, and chondrocytes. In this review, we will summarize signaling pathways and cytokines in chondrogenic differentiation of MSCs. Understanding the molecular mechanism of chondrogenic differentiation of CSCs and cancer cells may provide new strategies for cancer treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-022-02598-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052535PMC
April 2022

SAturation-recovery and Variable-flip-Angle-based three-dimensional free-breathing cardiovascular magnetic resonance T mapping at 3 T.

NMR Biomed 2022 Apr 29:e4755. Epub 2022 Apr 29.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.

The purpose of the current study was to develop and validate a three-dimensional (3D) free-breathing cardiac T -mapping sequence using SAturation-recovery and Variable-flip-Angle (SAVA). SAVA sequentially acquires multiple electrocardiogram-triggered volumes using a multishot spoiled gradient-echo sequence. The first volume samples the equilibrium signal of the longitudinal magnetization, where a flip angle of 2° is used to reduce the time for the magnetization to return to equilibrium. The succeeding three volumes are saturation prepared with variable delays, and are acquired using a 15° flip angle to maintain the signal-to-noise ratio. A diaphragmatic navigator is used to compensate the respiratory motion. T is calculated using a saturation-recovery model that accounts for the flip angle. We validated SAVA by simulations, phantom, and human subject experiments at 3 T. SAVA was compared with modified Look-Locker inversion recovery (MOLLI) and saturation-recovery single-shot acquisition (SASHA) in vivo. In phantoms, T by SAVA had good agreement with the reference (R  = 0.99). In vivo 3D T mapping by SAVA could achieve an imaging resolution of 1.25 × 1.25 × 8 mm . Both global and septal T values by SAVA (1347 ± 37 and 1332 ± 42 ms) were in between those by SASHA (1612 ± 63 and 1618 ± 51 ms) and MOLLI (1143 ± 59 and 1188 ± 65 ms). According to the standard deviation (SD) and coefficient of variation (CV), T precision measured by SAVA (SD: 99 ± 14 and 60 ± 8 ms; CV: 7.4% ± 0.9% and 4.5% ± 0.6%) was comparable with MOLLI (SD: 99 ± 25 and 46 ± 12 ms; CV: 8.8% ± 2.5% and 3.9% ± 1.1%) and superior to SASHA (SD: 222 ± 89 and 132 ± 33 ms; CV: 13.8% ± 5.5% and 8.1% ± 2.0%). It was concluded that the proposed free-breathing SAVA sequence enables more efficient 3D whole-heart T estimation with good accuracy and precision.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/nbm.4755DOI Listing
April 2022

Determines Nuclear Morphology During Syncytialization of Human Trophoblast Stem Cells.

Front Cell Dev Biol 2022 11;10:836390. Epub 2022 Apr 11.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Upon implantation, the trophectoderm differentiates into the multi-nucleated primitive syncytiotrophoblast (pSTB) through a process called primary syncytialization to facilitate maternal-fetal interactions and to establish a pregnancy. However, ethical issues and limited access to human embryos around the time of embryo implantation hinder the investigation of the detailed molecular mechanisms underpinning this event in humans. Here we established human trophoblast stem cells (hTSCs) from human blastocysts. We characterized nuclear enlargement in STB differentiated from hTSCs, which recapitulate morphological nuclear features of pSTB in human embryos. Specifically, we revealed that CRISPR/Cas9-mediated disruption perturbated nuclear volume during hTSCs syncytialization. Overall, our results not only provide an interesting insight into mechanisms underlying nuclear enlargement during primary syncytialization but highlight the hTSCs as an indispensable model in understanding human trophoblast differentiation during implantation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2022.836390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035786PMC
April 2022

Step-doped disulfide vacancies and functional groups synergistically enhance photocatalytic activity of S-scheme CuSnS/L-BiOBr towards ciprofloxacin degradation.

Chemosphere 2022 Aug 23;301:134684. Epub 2022 Apr 23.

School of Resources, Environment and Materials, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, MOE Key Laboratory of New Processing Technology for Non-ferrous Metals and Materials, Nanning, 530004, China. Electronic address:

Development of efficient photocatalysts for efficient recalcitrant organic pollutants degradation is of great significance. Herein, the step-doped disulfide vacancies S-scheme CuSnS/L-BiOBr (CTS/L-BiOBr) heterojunction photocatalyst was prepared for ciprofloxacin (CIP) degradation. X-ray photoelectron spectroscopy (XPS) analysis, ultraviolet photo-electron spectroscopy (UPS) analysis, band structure and dominant radicals' identification together verified that the transfer of photogenerated carriers conformed to the S-scheme mechanism. Benefited from the interfacial electric field (IEF) of the S-scheme heterojunction and incorporation of L-cysteine with introducing S-vacancies and surface functional groups (-NH, -COO), photogenerated charges generation and separation of the CTS/L-BiOBr were greatly improved. With ·OH and h as dominant reactive species, CIP removal reached 93% using CTS/L-BiOBr within 180 min of visible light irradiation, which was 3.5 times and 2.6 times of pristine CuSnS and L-BiOBr, respectively. Moreover, possible CIP degradation pathways were proposed and the degradation intermediates ecotoxicity were evaluated. This study could provide reference for designing efficient S-scheme photocatalysts for recalcitrant wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2022.134684DOI Listing
August 2022

Niosomal Nanocarriers for Enhanced Dermal Delivery of Epigallocatechin Gallate for Protection against Oxidative Stress of the Skin.

Pharmaceutics 2022 Mar 28;14(4). Epub 2022 Mar 28.

School of Pharmacy, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1023, New Zealand.

Among green tea catechins, epigallocatechin gallate (EGCG) is the most abundant and has the highest biological activities. This study aims to develop and statistically optimise an EGCG-loaded niosomal system to overcome the cutaneous barriers and provide an antioxidant effect. EGCG-niosomes were prepared by thin film hydration method and statistically optimised. The niosomes were characterised for size, zeta potential, morphology and entrapment efficiency. Ex vivo permeation and deposition studies were conducted using full-thickness human skin. Cell viability, lipid peroxidation, antioxidant enzyme activities after UVA-irradiation and cellular uptake were determined. The optimised niosomes were spherical and had a relatively uniform size of 235.4 ± 15.64 nm, with a zeta potential of -45.2 ± 0.03 mV and an EE of 53.05 ± 4.46%. The niosomes effectively prolonged drug release and demonstrated much greater skin penetration and deposition than free EGCG. They also increased cell survival after UVA-irradiation, reduced lipid peroxidation, and increased the antioxidant enzymes' activities in human dermal fibroblasts (Fbs) compared to free EGCG. Finally, the uptake of niosomes was via energy-dependent endocytosis. The optimised niosomes have the potential to be used as a dermal carrier for antioxidants and other therapeutic compounds in the pharmaceutical and cosmetic industries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics14040726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029719PMC
March 2022

Strategies of engineering nanomedicines for tumor retention.

J Control Release 2022 Jun 23;346:193-211. Epub 2022 Apr 23.

State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Pharmacy, Fudan University, Shanghai 201203, China.; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The retention of therapeutic agents in solid tumors at sufficient concentration and duration is crucial for their antitumor effects. Given the important contribution of nanomedicines to oncology, we herein summarized two major strategies of nanomedicines for tumor retention, such as transformation- and interactions-mediated strategies. The transformation-mediated retention strategy was achieved by enlarging particle size of nanomedicines or modulating the morphology into fibrous structures, while the interactions-mediated retention strategy was accomplished by modulating nanomedicines to promote their interactions with versatile cells or components in tumors. Moreover, we provide some considerations and perspectives of tumor-retaining nanomedicines for effective cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2022.04.006DOI Listing
June 2022

Erratum: PD-L1 expression in colon cancer and its relationship with clinical prognosis.

Int J Clin Exp Pathol 2022 15;15(3):155-156. Epub 2022 Mar 15.

Department of General Surgery, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University Xi'an 710004, Shaanxi, China.

[This corrects the article on p. 1764 in vol. 12, PMID: 31933995.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8986471PMC
March 2022
-->