Publications by authors named "Shun Yang"

73 Publications

Intelligent Assistant Diagnosis System of Osteosarcoma MRI Image Based on Transformer and Convolution in Developing Countries.

IEEE J Biomed Health Inform 2022 Aug 3;PP. Epub 2022 Aug 3.

Osteosarcoma is a malignant bone tumor commonly found in adolescents or children, with high incidence and poor prognosis. Magnetic resonance imaging (MRI), which is the more common diagnostic method for osteosarcoma, has a very large number of output images with sparse valid data and may not be easily observed due to brightness and contrast problems, which in turn makes manual diagnosis of osteosarcoma MRI images difficult and increases the rate of misdiagnosis. Current image segmentation models for osteosarcoma mostly focus on convolution, whose segmentation performance is limited due to the neglect of global features. In this paper, we propose an intelligent assisted diagnosis system for osteosarcoma, which can reduce the burden of doctors in diagnosing osteosarcoma from three aspects. First, we construct a classification-image enhancement module consisting of resnet18 and DeepUPE to remove redundant images and improve image clarity, which can facilitate doctors' observation. Then, we experimentally compare the performance of serial, parallel, and hybrid fusion transformer and convolution, and propose a Double U-shaped visual transformer with convolution (DUconViT) for automatic segmentation of osteosarcoma to assist doctors' diagnosis. This experiment utilizes more than 80,000 osteosarcoma MRI images from three hospitals in China. The results show that DUconViT can better segment osteosarcoma with DSC 2.6% and 1.8% higher than Unet and Unet++, respectively. Finally, we propose the pixel point quantification method to calculate the area of osteosarcoma, which provides more reference basis for doctors' diagnosis. Code: https://github.com/lingziqiang/DUconViT.
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http://dx.doi.org/10.1109/JBHI.2022.3196043DOI Listing
August 2022

[Wrist arthroscopy assisted open reduction and volar ligament reconstruction through osseous internal fixation for the treatment of distal radius Fernandez type Ⅳ fractures].

Zhongguo Gu Shang 2022 Jun;35(6):566-71

Sichuan Province Orthopaedics Hospital, Chengdu 610041, Sichuan, China.

Objective: To explore clinical effect of treatment for distal radius Fernandez type Ⅳ fractures by wrist arthroscopy assisted open reduction and volar ligament reconstruction through osseous, internal fixation.

Methods: From January 2018 to December 2019, 11 patients distal radius Fernandez type Ⅳ fractures treated with wrist arthroscopy assisted open reduction and volar ligament reconstruction through osseous, internal fixation, including 8 males and 3 females, aged from 23 to 42 years old. The fracture were all calssified to type Ⅳ according to Fernandez classification. Postoperative fracture healing, activity of wrist joint and recovery of wrist joint were observed, postoperative visual analogue scale(VAS) at 12 months was used to evaluate pain of wrist joint, and Cooney wrist score scale was used to evaluate clinical effects.

Results: All the 11 patients were followed up from 12 to 13 months, and fractures were healed well for 4 to 6 months. Postoperative VAS at 12 months ranged from 0 to 3, palm tilt angle ranged from 0° to 15 °, ulnar drift angle ranged from 15° to 21°, wrist flexion and extension motion ranged from 110° to 162°, forearm rotation motion ranged from 165° to 178°. Cooney wrist joint comprehensive score ranged from 70 to 95 points, and 8 patients got excellent result, 2 good, and 1 fair. No osteoarthritis and complications occurred.

Conclusion: Wrist arthroscopy assisted open reduction and volar ligament reconstruction through osseous, internal fixation for the treatment of Fernandez Ⅳ distal radius fractures could recevie effective fixation, and could receive satisfied clinical effect with system function exercise postoperative.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2022.06.012DOI Listing
June 2022

15-Hydroxyprostaglandin Dehydrogenase Is a Predictor of Stroke-Associated Pneumonia.

Front Neurol 2022 26;13:893624. Epub 2022 May 26.

Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China.

Background And Purpose: Stroke is a serious fatal and disabling disease. Stroke-associated pneumonia (SAP) is the most common complication of stroke, which may further aggravate the stroke. The prevention and early prediction of SAP is a key clinical strategy. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is involved in pneumonia, while its relationship with SAP has yet to be determined. Therefore, we investigated the predictive value of 15-PGDH for SAP and visualized their relationship.

Methods: Stroke patients were recruited and divided into SAP group and Non-SAP group. Baseline demographic and clinical data were obtained from the medical record system, blood samples were collected to detect relevant variables and 15-PGDH levels. Patient characteristics were compared with a t-test. Binary logistic regression analysis was performed to determine the predictive value of 15-PGDH for SAP. Restricted cubic splines (RCS) were performed to visualize the relationship between 15-PGDH and SAP risk. Finally, the SAP patient characteristics between the severe group and mild group were compared.

Results: 50 patients were enrolled and divided into SAP group ( = 26) and Non-SAP group ( = 24). 15-PGDH in the SAP group was lower than that in the Non-SAP group (0.258 ± 0.275 vs. 0.784 ± 0.615, = 0.025). Binary logistic regression analysis revealed that the lower 15-PGDH, the higher the risk of SAP (OR = 0.04, 95%CI, 0.010-0.157, < 0.001). The RCS model showed the L-shaped relationship between 15-PGDH and SAP.

Conclusions: In stroke patients, serum 15-PGDH is a valuable biomarker for predicting SAP. There is an L-shaped relationship between the level of 15-PGDH and the risk of SAP.
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http://dx.doi.org/10.3389/fneur.2022.893624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202497PMC
May 2022

Rethinking U-Net from an Attention Perspective with Transformers for Osteosarcoma MRI Image Segmentation.

Comput Intell Neurosci 2022 6;2022:7973404. Epub 2022 Jun 6.

School of Computer Science and Engineering, Central South University, Changsha 410083, China.

Osteosarcoma is one of the most common primary malignancies of bone in the pediatric and adolescent populations. The morphology and size of osteosarcoma MRI images often show great variability and randomness with different patients. In developing countries, with large populations and lack of medical resources, it is difficult to effectively address the difficulties of early diagnosis of osteosarcoma with limited physician manpower alone. In addition, with the proposal of precision medicine, existing MRI image segmentation models for osteosarcoma face the challenges of insufficient segmentation accuracy and high resource consumption. Inspired by transformer's self-attention mechanism, this paper proposes a lightweight osteosarcoma image segmentation architecture, UATransNet, by adding a multilevel guided self-aware attention module (MGAM) to the encoder-decoder architecture of U-Net. We successively perform dataset classification optimization and remove MRI image irrelevant background. Then, UATransNet is designed with transformer self-attention component (TSAC) and global context aggregation component (GCAC) at the bottom of the encoder-decoder architecture to perform integration of local features and global dependencies and aggregation of contexts to learned features. In addition, we apply dense residual learning to the convolution module and combined with multiscale jump connections, to improve the feature extraction capability. In this paper, we experimentally evaluate more than 80,000 osteosarcoma MRI images and show that our UATransNet yields more accurate segmentation performance. The IOU and DSC values of osteosarcoma are 0.922 ± 0.03 and 0.921 ± 0.04, respectively, and provide intuitive and accurate efficient decision information support for physicians.
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http://dx.doi.org/10.1155/2022/7973404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192230PMC
June 2022

Synthesis and anti-inflammatory activity of paeonol derivatives with etherized aryl urea by regulating TLR4/MyD88 signaling pathway in RAW264.7 cell.

Bioorg Chem 2022 Oct 9;127:105939. Epub 2022 Jun 9.

College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, PR China. Electronic address:

Thirty-three novel paeonol etherized aryl urea derivatives (PEUs) were synthesized via a bromination-Williamson Ether Synthesis-deprotection-nucleophilic addition reaction sequence. The structures of PEUs were characterized by LC-MS, HRMS, H NMR and C NMR spectra. The levels of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages were initially employed to evaluate the anti-inflammatory effects of all compounds. Remarkably, b16 exhibited a good anti-inflammatory activity at 2.5 μm which is the same as the potency of paeonol at 20 μm. The results of mechanism research displayed that the anti-inflammatory effect of b16 was ascribed to the inhibition of the TLR4/MyD88 signaling pathway and inflammatory factors. Additionally, b16 distinctly reduced the generation of free radicals in macrophages and strikingly increased the mitochondrial membrane potential. According to the structure-activity relationships (SAR) of PEUs, the incorporation of halogens on the benzene ring and the hydrogen of phenol hydroxyl substituted by aryl urea, were beneficial to enhance the anti-inflammatory activities. Molecular docking results illustrated that the binding ability of b16 to TLR4 was stronger than that of paeonol. In summary, the novel aryl urea-derivied paeonol b16 could be a new promising candidate for the treatment of inflammation-related diseases.
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http://dx.doi.org/10.1016/j.bioorg.2022.105939DOI Listing
October 2022

Osteoimmune reaction caused by a novel silicocarnotite bioceramic promoting osteogenesis through the MAPK pathway.

Biomater Sci 2022 May 31;10(11):2877-2891. Epub 2022 May 31.

Department of Orthodontics, School and Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, Shanghai 200072, China.

The host immune response to an implant is a key factor in determining the fate of bone grafts, which is thought to be a regulator of tissue regeneration. Figuring out the effects of the osteoimmune microenvironment on the osteogenesis of bone grafts can be a valuable strategy for their design and can further enhance the healing of bone defects. Our previous study demonstrated that the silicocarnotite (Ca(PO)SiO, CPS) bioceramic can significantly promote osteogenesis. The aim of this study is to investigate the immune reaction of CPS, the effects of the immune microenvironment on osteogenesis, and the related molecular mechanisms. Compared to hydroxyapatite (Ca(PO)(OH), HA), the results showed that CPS could downregulate the pro-inflammatory phenotype and upregulate the anti-inflammatory phenotype, showing the lower levels of TNF-α and increased expression of IL-10. We further found that CPS could regulate the expression of NPPA, EDN1, and MMP9 in RAW 264.7 by RNA sequencing, which may be related to its superiority in osteogenesis. The osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) was subsequently studied in a macrophage-conditioned medium pretreated with CPS, and the medium caused a significant promotion of the osteogenic differentiation of rBMSCs, demonstrating that CPS can generate a favorable immune microenvironment to promote rBMSCs differentiation. In terms of mechanism, CPS in the macrophage-conditioned medium promoted osteogenic differentiation through the MAPK pathway, including ERK1/2, JNK and P38. Our study demonstrated that osteogenic differentiation was influenced by the immune microenvironment generated the implant, and also presented an effective tool for studying the mechanisms of macrophage polarization as well as functions.
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http://dx.doi.org/10.1039/d2bm00125jDOI Listing
May 2022

Co-exchanged montmorillonite: a potential antibacterial agent with good antibacterial activity and cytocompatibility.

J Mater Chem B 2022 05 18;10(19):3705-3715. Epub 2022 May 18.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.

As a biocompatible material with rich resources and economic benefits, montmorillonite (MMT) has been widely used in the antibacterial field as a drug carrier and toxin adsorbent. In addition, the distinctive structure of MMT provides a possibility to tune its property in a wide range through ion-exchange. In this study, Co-montmorillonite (CoMMT) was prepared by the ion-exchanging method in a Co(NO) solution and its antibacterial activity and cytocompatibility were investigated. The results showed that Co was introduced into MMT successfully and led to an increase in the interlayer spacing of MMT. Also, CoMMT showed a morphology of irregular aggregates consisting of stacked and intertwined lamellae with a uniform cobalt distribution. Besides, CoMMT had better dispersity and higher specific surface area than unmodified MMT. The antibacterial test results showed that CoMMT had good antibacterial activity against and when the CoMMT concentration was higher than 0.2 mg mL and 0.4 mg mL, respectively. The possible antibacterial mechanism of CoMMT was speculated and verified by a combination of SEM and EDS results. In addition, CoMMT showed no obvious cytotoxicity to MC3TC-E1 at the observed antibacterial concentration. These findings demonstrated that CoMMT with good biocompatibility and antibacterial activity could be used as a novel antibacterial agent for tissue engineering.
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http://dx.doi.org/10.1039/d2tb00032fDOI Listing
May 2022

Intelligent Segmentation Medical Assistance System for MRI Images of Osteosarcoma in Developing Countries.

Comput Math Methods Med 2022 19;2022:7703583. Epub 2022 Jan 19.

Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China.

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. It has a high degree of malignancy and a poor prognosis in developing countries. The doctor manually explained that magnetic resonance imaging (MRI) suffers from subjectivity and fatigue limitations. In addition, the structure, shape, and position of osteosarcoma are complicated, and there is a lot of noise in MRI images. Directly inputting the original data set into the automatic segmentation system will bring noise and cause the model's segmentation accuracy to decrease. Therefore, this paper proposes an osteosarcoma MRI image segmentation system based on a deep convolution neural network, which solves the overfitting problem caused by noisy data and improves the generalization performance of the model. Firstly, we use Mean Teacher to optimize the data set. The noise data is put into the second round of training of the model to improve the robustness of the model. Then, we segment the image using a deep separable U-shaped network (SepUNet) and conditional random field (CRF). SepUnet can segment lesion regions of different sizes at multiple scales; CRF further optimizes the boundary. Finally, this article calculates the area of the tumor area, which provides a more intuitive reference for assisting doctors in diagnosis. More than 80000 MRI images of osteosarcoma from three hospitals in China were tested. The results show that the proposed method guarantees the balance of speed, accuracy, and cost under the premise of improving accuracy.
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http://dx.doi.org/10.1155/2022/7703583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791734PMC
March 2022

COX-2/PGE2 Pathway Inhibits the Ferroptosis Induced by Cerebral Ischemia Reperfusion.

Mol Neurobiol 2022 Mar 10;59(3):1619-1631. Epub 2022 Jan 10.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Cerebral ischemia reperfusion (I/R) injury easily develops in ischemic stroke, resulting in more serious injury. Ferroptosis is involved in cerebral I/R injury, but the mechanism remains unclear. Prostaglandin E2 (PGE2) is potential to regulate ferroptosis. This study mainly explored the regulation effects of PGE2 on ferroptosis induced by cerebral I/R. We first detected PGE2 levels and ferroptosis status in 11 human brain tissues. Then, we induced a cerebral I/R animal model to examine ferroptosis status in cerebral I/R. We further injected a ferroptosis inhibitor to define the response of the PGE2 pathway to ferroptosis. Finally, we injected PGE2 and pranoprofen to explore the regulation of the cyclooxygenases 2 (COX-2)/PGE2 pathway on ferroptosis in cerebral I/R. We found that PGE2 release was correlated with the levels of reactive oxygen species, malondialdehyde, glutathione peroxidase 4, COX-2, and Spermidine/spermine N1-acetyltransferase 1. Ferroptosis can be induced by cerebral I/R, while inhibition of ferroptosis induced by cerebral I/R can inactivate PGE2 synthases, degrade enzyme, and parts of PGE2 receptors, and reduce cerebral infarct volume. In turn, PGE2 inhibited ferroptosis through the reduction of Fe, glutathione oxidation, and lipid peroxidation, while pranoprofen, one of the COX inhibitors, played an opposite role. In conclusion, PGE2 was positively correlated with ferroptosis, inhibition of ferroptosis induced by cerebral I/R can inactivate COX-2/PGE2 pathway, and PGE2 inhibited ferroptosis induced by cerebral I/R, possibly via PGE2 receptor 3 and PGE2 receptor 4. Graphical abstract Inhibition of ferroptosis inactivates the COX-2/PGE2 pathway. Cerebral ischemia reperfusion injury induces the secretion of PGE2. After the inhibition of ferroptosis by Fer-1, the expression of cyclooxygenases (COX-1 and COX-2) decreased, and PGE2 synthases cPGES, mPGES-1, and mPGES-2 were also reduced. At the same time, the PGE2 degradation enzyme 15-PGDH was also reduced. Changes in these enzymes ultimately result in the declination of PGE2. Besides, the expression of PGE2 receptors EP3 and EP4 is also inhibited, indicating that the function they mediate is also impaired. In conclusion, after cerebral ischemia reperfusion injury, the inhibition of ferroptosis inactivates the COX-2/PGE2 pathway.
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http://dx.doi.org/10.1007/s12035-021-02706-1DOI Listing
March 2022

A smart enzyme reactor based on a photo-responsive hydrogel for purifying water from phenol contaminated sources.

Soft Matter 2022 Jan 26;18(4):826-831. Epub 2022 Jan 26.

School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.

In this paper, a smart enzyme reactor (SER) was synthesized using immobilized tyrosinase (Tyr) in a photo-responsive hydrogel a polydopamine-assisted self-assembly strategy for purifying water from phenol contaminated water. PDA was not only utilized as a binder between Tyr and the hydrogel to prevent the leakage of Tyr with relatively high enzymatic activity from the SER, but also acted as a light absorber to trigger the hydrophilic/hydrophobic switching of PNIPAm hydrogels to realize the efficient reclamation of clean water. Experimental results showed that the SER maintained a well-defined porous structure with excellent elasticity, which was beneficial for water transport and enzyme accessibility. And the stability and reusability of Tyr in the degradation of phenol were all improved. Furthermore, clean water could be reclaimed completely and facilely by light irradiation after enzymatic remediation in the SER.
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http://dx.doi.org/10.1039/d1sm01536bDOI Listing
January 2022

Two phenanthroimidazole turn-on probes for the rapid detection of selenocysteine and its application in living cells imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Feb 6;267(Pt 2):120585. Epub 2021 Nov 6.

Key Laboratory of Theoretical Organic Chemistry and Function Molecule of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China. Electronic address:

Detection of selenocysteine (Sec) content in cells by fluorescence probe is of great significance for the identification of human related diseases. To achieve fast and sensitive detection of Sec, two isomers A4 and B4 as turn-on fluorescent probes to detect Sec were designed and synthesized. Both A4 and B4 display fast turn-on response, high selectivity and sensitivity toward Sec, which can be applied for fluorescence imaging of Sec in living cells. Compared with B4, A4 has a larger Stokes shift (125 nm), wider pH range (5-10) and lower detection limit (65.4 nM) due to its ESIPT (excited state intramolecular proton transfer) effect. In view of the detection performance of these two probes, they can be used as effective tools for detecting Sec in biological systems.
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http://dx.doi.org/10.1016/j.saa.2021.120585DOI Listing
February 2022

High-resolution fast-tomography brain-imaging beamline at the Taiwan Photon Source.

J Synchrotron Radiat 2021 Sep 23;28(Pt 5):1662-1668. Epub 2021 Aug 23.

Institute of Physics, Academia Sinica, 128 Academia Road Sec. 2, Taipei 11529, Taiwan.

The new Brain Imaging Beamline (BIB) of the Taiwan Photon Source (TPS) has been commissioned and opened to users. The BIB and in particular its endstation are designed to take advantage of bright unmonochromatized synchrotron X-rays and target fast 3D imaging, ∼1 ms exposure time plus very high ∼0.3 µm spatial resolution. A critical step in achieving the planned performances was the solution to the X-ray induced damaging problems of the detection system. High-energy photons were identified as their principal cause and were solved by combining tailored filters/attenuators and a high-energy cut-off mirror. This enabled the tomography acquisition throughput to reach >1 mm min, a critical performance for large-animal brain mapping and a vital mission of the beamline.
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http://dx.doi.org/10.1107/S1600577521007633DOI Listing
September 2021

Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM.

EMBO Rep 2021 09 2;22(9):e52911. Epub 2021 Aug 2.

Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada.

Cilia are thin microtubule-based protrusions of eukaryotic cells. The swimming of ciliated protists and sperm cells is propelled by the beating of cilia. Cilia propagate the flow of mucus in the trachea and protect the human body from viral infections. The main force generators of ciliary beating are the outer dynein arms (ODAs) which attach to the doublet microtubules. The bending of cilia is driven by the ODAs' conformational changes caused by ATP hydrolysis. Here, we report the native ODA complex structure attaching to the doublet microtubule by cryo-electron microscopy. The structure reveals how the ODA complex is attached to the doublet microtubule via the docking complex in its native state. Combined with coarse-grained molecular dynamic simulations, we present a model of how the attachment of the ODA to the doublet microtubule induces remodeling and activation of the ODA complex.
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http://dx.doi.org/10.15252/embr.202152911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419702PMC
September 2021

Exploring the Pathogenesis of Psoriasis Complicated With Atherosclerosis Microarray Data Analysis.

Front Immunol 2021 27;12:667690. Epub 2021 May 27.

Department of Dermatology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Background: Although more and more evidence has supported psoriasis is prone to atherosclerosis, the common mechanism of its occurrence is still not fully elucidated. The purpose of this study is to further explore the molecular mechanism of the occurrence of this complication.

Methods: The gene expression profiles of psoriasis (GSE30999) and atherosclerosis (GSE28829) were downloaded from the Gene Expression Omnibus (GEO) database. After identifying the common differentially expressed genes (DEGs) of psoriasis and atherosclerosis, three kinds of analyses were performed, namely functional annotation, protein-protein interaction (PPI) network and module construction, and hub gene identification and co-expression analysis.

Results: A total of 94 common DEGs (24 downregulated genes and 70 upregulated genes) was selected for subsequent analyses. Functional analysis emphasizes the important role of chemokines and cytokines in these two diseases. In addition, lipopolysaccharide-mediated signaling pathway is closely related to both. Finally, 16 important hub genes were identified using cytoHubba, including LYN, CSF2RB, IL1RN, RAC2, CCL5, IRF8, C1QB, MMP9, PLEK, PTPRC, FYB, BCL2A1, LCP2, CD53, NCF2 and TLR2.

Conclusions: Our study reveals the common pathogenesis of psoriasis and atherosclerosis. These common pathways and hub genes may provide new ideas for further mechanism research.
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http://dx.doi.org/10.3389/fimmu.2021.667690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190392PMC
October 2021

An L314Q mutation in Map3k1 gene results in failure of eyelid fusion in the N-ethyl-N-nitrosourea-induced mutant line.

Exp Anim 2021 Nov 2;70(4):459-468. Epub 2021 Jun 2.

Institute of Comparative Medicine, Yangzhou University, 12 Wenhui East Road, Yangzhou, Jiangsu Province 225009, P.R.China.

In this study, we describe an N-ethyl-N-nitrosourea-induced mouse model with a corneal opacity phenotype that was associated with "eye open at birth" (EOB). Histological and immunohistochemistry staining analysis showed abnormal differentiation of the corneal epithelial cells in the mutant mice. The EOB phenotype was dominantly inherited on a C57BL/6 (B6) background. This allele carries a T941A substitution in exon 4 that leads to an L314Q amino acid change in the open reading frame of MAP3K1 (MEEK1). We named this novel Map3k1 allele Map3k1. Phalloidin staining of F-actin was reduced in the mutant epithelial leading edge cells, which is indicative of abnormality in epithelial cell migration. Interestingly enough, not only p-c-Jun and p-JNK but also c-Jun levels were decreased in the mutant epithelial leading edge cells. This study identifies a novel mouse Map3k1 allele causing EOB phenotype and the EOB phenotype in Map3k1 mouse may be associated with the reduced level of p-JNK and c-Jun.
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http://dx.doi.org/10.1538/expanim.21-0005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614015PMC
November 2021

Effects of reductive inorganics and NOM on the formation of chlorite in the chlorine dioxide disinfection of drinking water.

J Environ Sci (China) 2021 Jun 22;104:225-232. Epub 2020 Dec 22.

Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science & Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Chlorine dioxide (ClO) disinfection usually does not produce halogenated disinfection by-products, but the formation of the inorganic by-product chlorite (ClO) is a serious consideration. In this study, the ClO formation rule in the ClO disinfection of drinking water was investigated in the presence of three representative reductive inorganics and four natural organic matters (NOMs), respectively. Fe and S mainly reduced ClO to ClO at low concentrations. When ClO was consumed, the ClO would be further reduced by Fe and S, leading to the decrease of ClO. The reaction efficiency of Mn with ClO was lower than that of Fe and S. It might be the case that MnO generated by the reaction between Mn and ClO had adsorption and catalytic oxidation on Mn. However, Mn would not reduce ClO. Among the four NOMs, humic acid and fulvic acid reacted with ClO actively, followed by bovine serum albumin, while sodium alginate had almost no reaction with ClO. The maximum ClO yields of reductive inorganics (70%) was higher than that of NOM (around 60%). The lower the concentration of reductive substances, the more ClO could be produced by per unit concentration of reductive substances. The results of the actual water samples showed that both reductive inorganics and NOM played an important role in the formation of ClO in disinfection.
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http://dx.doi.org/10.1016/j.jes.2020.11.033DOI Listing
June 2021

C-type lectin with a QPN motif from swimming crab Portunus trituberculatus displays broad nonself-recognition ability and functions as an opsonin.

Dev Comp Immunol 2021 07 9;120:104066. Epub 2021 Mar 9.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedcine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.

In the immune system, C-type lectins, as pattern recognition receptors, have an important function. Carbohydrate-recognition domains (CRDs) endow C-type lectins with the function of recognizing and scavenging non-self factors. In the present study, a new C-type lectin (designated as PtCTL-9 according to the order of discovery) from swimming crab (Portunus trituberculatus) was characterized. QPN (Gln-Pro-Asn) and FHS (Phe-His-Ser) were identified as the key motifs that determine carbohydrate binding. Motif QPN was mutated to QPD (Gln-Pro-Asp) (M1) and EPN (Glu-Pro-Asn) (M2) to study its immune function and for comparative analysis. The results showed that PtCTL-9 displayed broad non-self immunity. PtCTL-9 could also function as an opsonin to promote phagocytosis and the in vitro encapsulation of hemocytes. These results indicated that PtCTL-9 has an extensive nonself-recognition ability, regulates pathogen clearance, and its QPN motif is important in PtCTL-9's immune function.
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http://dx.doi.org/10.1016/j.dci.2021.104066DOI Listing
July 2021

Rational design to enhance the catalytic activity of 2-deoxy-D-ribose-5-phosphate aldolase from Pseudomonas syringae pv. syringae B728a.

Protein Expr Purif 2021 07 5;183:105863. Epub 2021 Mar 5.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, 310018, China; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China. Electronic address:

The 2-Deoxy-d-ribose-5-phosphate aldolase (DERA) enzyme in psychrophilic bacteria has gradually attracted the attention of researchers. A novel gene, deoC (681 bp), encoding DERA, was identified in Pseudomonas syringae pv. syringae B728a, recombinantly expressed in E. coli BL21 and purified via affinity chromatography, which yielded a homodimeric enzyme of 23 kDa. The specific activity of DERA toward 2-deoxy-d-ribose-5-phosphate (DR5P) was 7.37 ± 0.03 U/mg, and 61.32% of its initial activity remained after incubation in 300 mM acetaldehyde at 25 °C for 2 h. Based on the calculation results (dock binding free energy) with the ligand chloroacetaldehyde (CAH), five target substitutions (T16L, F69R, V66K, S188V, and G189R) were identified, in which the DERA mutant (G189R) exhibited higher catalytic activity toward DR5P than DERA. Only the DERA mutant (V66K) exhibited 12% higher activity toward chloroacetaldehyde and acetaldehyde condensation reactions than DERA. Fortunately, the aldehyde tolerance of these mutants exhibited no significant decline compared with the wild type. These results indicate an effective strategy for enhancing DERA activity.
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http://dx.doi.org/10.1016/j.pep.2021.105863DOI Listing
July 2021

Molecular cloning and binding analysis of polymeric immunoglobulin receptor in largemouth bass (Micropterus salmoides).

Mol Immunol 2021 05 18;133:14-22. Epub 2021 Feb 18.

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, 310018, China; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

The polymeric immunoglobulin receptor (pIgR) is an important molecule in the mucosal immunity of teleosts. Previous studies have shown that pIgR can bind and transport polymeric immunoglobulins (pIgs), but few studies have focused on the binding of teleost pIgR to bacteria. In this study, we identified a gene encoding pIgR in largemouth bass (Micropterus salmoides). The pIgR gene contained two Ig-like domains (ILDs), which were homologous to ILD1 and ILD5 of mammalian pIgR. Our results showed that largemouth bass pIgR-ILD could combine with IgM. Moreover, we also found that largemouth bass pIgR-ILD could bind to Aeromonas hydrophila and Micrococcus luteus. Further analysis showed that largemouth bass pIgR-ILD could also combine with lipopolysaccharide (LPS), peptidoglycan (PGN) and various saccharides, and reduced binding to bacteria was observed with LPS and PGN treatment, indicating that largemouth bass pIgR could bind to bacteria to prevent infection and that saccharide binding is an important interaction mechanism between pIgR and bacteria. These results collectively demonstrated that largemouth bass pIgR not only combines with IgM but also binds to bacteria by various saccharides.
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http://dx.doi.org/10.1016/j.molimm.2021.02.001DOI Listing
May 2021

Progress in the role of high resolution magnetic resonance imaging technology in the risk assessment of intracranial aneurysm rupture.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Dec;45(12):1476-1482

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China.

The traditional classification, diagnosis, and treatment of intracranial aneurysms are based on the characteristics of their vascular lumen. However, in the past few years, some advances in MRI technology with high-resolution imaging can assess the pathology of intracranial vascular walls. Compared with traditional methods of computed tomography angiography, magnetic resonance angiograhpy, and digital subtraction angiography, high resolution magnetic resonance imaging technology can help us to newly understand the disease by directly evaluating the characteristics of vascular wall, such as aneurysm wall thickness, inflammation, enhancement, permeability and hemodynamics. At present, high-resolution magnetic resonance imaging is increasingly used in clinic to assess the rupture risk of intracranial aneurysms, which is of great significance for guiding the diagnosis and treatment of intracranial aneurysms.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.190676DOI Listing
December 2020

Dendrite-free Zn anodes enabled by functional nitrogen-doped carbon protective layers for aqueous zinc-ion batteries.

Dalton Trans 2020 Dec;49(48):17629-17634

School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, P.R. China.

Rechargeable aqueous zinc-ion batteries possess the merits of good environmental benignity, high operational safety and high energy density. Nevertheless, the practical application of zinc-ion batteries is severely obstructed by the inhomogeneous deposition of metallic Zn on the anode, which results in serious capacity fading, poor coulombic efficiency, and electrolyte consumption. Herein, we propose a simple strategy of constructing a functional nitrogen-doped carbon network coating layer on zinc foil for dendrite-free Zn stripping/plating. On one hand, the good conductivity of the artificial Zn/electrolyte interface can quickly balance the electric field and lower the nucleation overpotential. On the other hand, the porosity feature and functional groups of the protective layer can provide a fast Zn2+ transportation pathway and generate well-dispersed nucleation seeds. Therefore, the protective layer can effectively hamper the growth of metallic Zn dendrites and resist side reactions. The as-prepared N-C/Zn anode displays superior cycling stability (800 h at 2 mA cm-2 with the capacity of 2 mA h cm-2) and a satisfactory coulombic efficiency of 98.76% during the Zn stripping/plating process. A long cycle life and high specific capacity (162.10 mA h g-1 after 500 cycles at 2.0 A g-1) are also obtained for N-C/Zn||ZnSO4||V2O5 full cells. The strategy provides a facile and effective opportunity for constructing high-performance rechargeable aqueous zinc-ion batteries.
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http://dx.doi.org/10.1039/d0dt03459bDOI Listing
December 2020

Unveiling the Hidden Diversity of Rock-Inhabiting Fungi: Chaetothyriales from China.

J Fungi (Basel) 2020 Sep 24;6(4). Epub 2020 Sep 24.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, China.

Rock-inhabiting fungi (RIF) are nonlichenized fungi that naturally colonize rock surfaces and subsurfaces. The extremely slow growth rate and lack of distinguishing morphological characteristics of RIF resulted in a poor understanding on their biodiversity. In this study, we surveyed RIF colonizing historical stone monuments and natural rock formations from throughout China. Among over 1000 isolates, after preliminary delimitation using the internal transcribed spacer region (ITS) sequences, representative isolates belonging to Trichomeriaceae and Herpotrichiellaceae were selected for a combined analysis of ITS and the nuclear ribosomal large subunit (nucLSU) to determine the generic placements. Eight clades representing seven known genera and one new genus herein named as were placed in Trichomeriaceae. While, for Herpotrichiellaceae, two clades corresponded to two genera: and . Fine-scale phylogenetic analyses using combined sequences of the partial actin gene (ACT), ITS, mitochondrial small subunit ribosomal DNA (mtSSU), nucLSU, the largest subunit of RNA polymerase II (RPB1), small subunit of nuclear ribosomal RNA gene (SSU), translation elongation factor (TEF), and β-tubulin gene (TUB) revealed that these strains represented 11 and 6 new species, respectively, in Trichomeriaceae and Herpotrichiellaceae. The 17 new species were described, illustrated for their morphologies and compared with similar taxa. Our study demonstrated that the diversity of RIF is surprisingly high and still poorly understood. In addition, a rapid strategy for classifying RIF was proposed to determine the generic and familial placements through preliminary ITS and nucLSU analyses, followed by combined analyses of five loci selected from ACT, ITS, mtSSU, nucLSU, RPB1, and/or the second subunit of RNA polymerase II gene (RPB2), SSU, TEF, and TUB regions to classify RIF to the species level.
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http://dx.doi.org/10.3390/jof6040187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711927PMC
September 2020

Photo-responsive degradable hollow mesoporous organosilica nanoplatforms for drug delivery.

J Nanobiotechnology 2020 Jun 15;18(1):91. Epub 2020 Jun 15.

School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, China.

Background: Stimulus-responsive degradable mesoporous organosilica nanoparticles (MONs) have shown great promise as drug carriers via enhancing the efficiency of drug delivery and accelerating the degradation of nanocarriers. However, it remains a great challenge to develop novel light-enabled spatial and temporal degradable MONs with both superior responsiveness for efficient anti-cancer drug delivery and safe exocytosis.

Results: We report a novel photo-responsive degradable hollow mesoporous organosilica nanoplatform ([email protected]). The platform is based on organosilica nanoparticles (HMONs) containing singlet oxygen (O)-responsive bridged organoalkoxysilanes and wrapped graphene oxide quantum dots (GOQDs). The unique hollow mesoporous structure of the HMONs guarantees an excellent drug loading and release profile. During light irradiation, O produced by the GOQDs leads to the degradation of the organosilica nanoparticles, resulting in enhanced local drug release.

Conclusions: We carried out in vitro and in vivo experiments using DOX as a model drug; [email protected] exhibited high biocompatibility, accelerated degradation, and superior therapeutic efficacy during light irradiation, indicating a promising platform for clinical cancer therapy.
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http://dx.doi.org/10.1186/s12951-020-00642-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296706PMC
June 2020

CircRNA 001372 Reduces Inflammation in Propofol-Induced Neuroinflammation and Neural Apoptosis through PIK3CA/Akt/NF-κB by miRNA-148b-3p.

J Invest Surg 2021 Nov 7;34(11):1167-1177. Epub 2020 Jun 7.

Department of Anesthesia, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning, China.

Objectives: To investigate effects of circular RNA (circRNA) 001372 and its antagonist miRNAs-148b-3p on propofol-induced neurotoxicity and neuroinflammation in rat brain and pheochromocytoma cells.

Methods: Sprague Dawley rats in propofol model group (n = 6) were intraperitoneal injected with propofol (50 mg/kg) and in sham control group (n = 6) without any treatment. Twenty-four h later, brain tissues were acquired during pentobarbital anesthesia. PC-12 cells were transfected with or without circRNA001372 mimics, circRNA001372 inhibitor, negative mimics or miRNA-148b-3p for 48 h and then treated with propofol (100 μM) for 48 h. Quantitative reverse transcription PCR and gene chips were used for detecting levels of circRNA001372, Haemotoxylin and Eosin staining for cell morphology, MTT for cell viability, flow cytometry for apoptosis, enzyme-linked immunosorbent assay for lactate dehydrogenase (LDH), interleukin-1β (IL-1β), IL-6, IL17 and IL-18, and Western blots for phosphoinositide 3-kinase (PI3K), Akt, phosphorylated Akt, and nuclear factor (NF) κB, dual-light luminescent reporter gene assay for luciferase reporter.

Results: The propofol anesthesia in rats decreases levels of circRNA001372 and increases levels of cytokines including IL-1β, IL-6, IL17 and IL-18, resulting in the neurocyte damage in brain. In propofol-treated PC-12 cells, the inhibition of circRNA001372 increases apoptosis and cell damage makers, including LDH, IL-1β, IL-6, IL17, IL-18, resulting in the reduction of cell viability, which have been revised after over-expression of circRNA001372. MiRNA-148b-3p reduces circRNA001372-incresed PI3K and pAKt levels but enhances the circRNA001372-decreased NFκB level.

Conclusions: CircRNA001372 suppresses propofol-induced neurotoxicity and neuroinflammation through PI3K/Akt/NF-κB signaling pathway in rat brain and neurocytes. MiRNA-148b-3p antagonizes the effects of circRNA001372.
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http://dx.doi.org/10.1080/08941939.2020.1771639DOI Listing
November 2021

[Open pulling reduction and bone graft by overstretched wrist traction and internal fixation for the treatment of distal radius type Fernandez Ⅲ fractures].

Zhongguo Gu Shang 2020 Apr;33(4):367-70

Department of Orthopaedic Trauma and Hand Surgery, the Orthopaedic Hospital of Sichuan Province, Chengdu 610041, Sichuan, China.

Objective: To explore the clinical effect of the treatment of Fernandez type Ⅲ fracture of the distal radius with hyperextension traction prying, volar reduction, bone grafting and internal fixation.

Methods: From February 2017 to March 2018, 11 cases of Fernandez type Ⅲ fracture of the distal radius were treated with intraoperative hyperextension traction and volar prying reduction and bone grafting and internal fixation, including 6 males and 5 females, aged 55 to 67 years. Preoperative X-ray and CT evaluated the distal radius fracture dorsal angulation with articular surface compression, collapse. According to Fernandez, all of them were type Ⅲ. After operation, the reduction of articular surface and fracture healing were evaluated. VAS score and Cooney wrist score were used to evaluate the curative effect.

Results: All the patients were followed up for 12 to 14 months. All the fractures healed. Cooney wrist score scale was used to evaluate the curative effect, 9 cases were excellent, 1 case was good and 1 case was fair.

Conclusion: In the operation of Fernandez type Ⅲ fracture of the distal radius, hyperextension traction was used to enlarge the angle, and through the volar fracture end prying reduction and internal fixation with bone graft, the collapsed articular surface could be effectively reduced and fixed. The early functional exercise after the operation had satisfactory clinical effect.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2020.04.015DOI Listing
April 2020

An Ultrastrong and Highly Stretchable Polyurethane Elastomer Enabled by a Zipper-Like Ring-Sliding Effect.

Adv Mater 2020 Jun 30;32(23):e2000345. Epub 2020 Apr 30.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Elastomers with excellent mechanical properties are in substantial demand for various applications, but there is always a tradeoff between their mechanical strength and stretchability. For example, partially replacing strong covalent crosslinking by weak sacrificial bonds can enhance the stretchability but also usually decreases the mechanical strength. To surmount this inherent tradeoff, a supramolecular strategy of introducing a zipper-like sliding-ring mechanism in a hydrogen-bond-crosslinked polyurethane network is proposed. A very small amount (0.5 mol%) of an external additive (pseudo[2]rotaxane crosslinker) can dramatically increase both the mechanical strength and elongation of this polyurethane network by nearly one order of magnitude. Based on the investigation of the relationship between molecular structure and mechanical properties, this enhancement is attributable to a unique molecular-level zipper-like ring-sliding motion, which efficiently dissipates mechanical work in the solvent-free network. This research not only provides a distinct and general strategy for the construction of high-performance elastomers but also paves the way for the practical application of artificial molecular machines toward solvent-free polyurethane networks.
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http://dx.doi.org/10.1002/adma.202000345DOI Listing
June 2020

[Wrist arthroscopic treatment of Diepunch fracture of distal radius].

Zhongguo Gu Shang 2020 Jan;33(1):59-63

Department of Wrist, Sichuan Orthopaedic Hospital, Chengdu 610041, Sichuan, China.

Objective: To evaluate the effect of open reduction assisted by wrist arthroscopy in the treatment of Diepunch fracture of the distal radius.

Methods: The clinical data of 50 patients with die punch fracture of distal radius from December 2015 to May 2017 were analyzed retrospectively, including 31 males and 19 females, aged 20 to 45 (34.12±2.56) years. All patients were treated with open reduction and internal fixation of volar plate through volar approach under the assistance of wrist arthroscope. The range of wrist movement and Cooney wrist function score before and after treatment were compared.

Results: All patients were followed up with an average of 18 months. DR scan showed that all fractures healed and no shortening of radial axis. Three cases of incision infection occurred and disappeared after treatment. At 18 months after operation, the range of wrist movement was significantly larger than that before operation (<0.05) . At 18 months after operation, Cooney wrist function score was higher than that before operation (<0.05) , excellent in 33 cases, good in 13 cases, fair in 3 cases and poor in 1 case.

Conclusion: The treatment of die punch fracture of the distal radius with open reduction assisted by arthroscopy can restore the flatness of the joint surface, promote the recovery of the function of the wrist joint quickly, and has high safety, which is worth popularizing.
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http://dx.doi.org/10.3969/j.issn.1003-0034.2020.01.011DOI Listing
January 2020

The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications.

Elife 2020 01 17;9. Epub 2020 Jan 17.

Department of Anatomy and Cell Biology, McGill University, Québec, Canada.

Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule. Microtubule also exists as highly stable doublet microtubules in the cilia where stability is needed for ciliary beating and function. The doublet microtubule maintains its stability through interactions at its inner and outer junctions where its A- and B-tubules meet. Here, using cryo-electron microscopy, bioinformatics and mass spectrometry of the doublets of and , we identified two new inner junction proteins, FAP276 and FAP106, and an inner junction-associated protein, FAP126, thus presenting the complete answer to the inner junction identity and localization. Our structural study of the doublets shows that the inner junction serves as an interaction hub that involves tubulin post-translational modifications. These interactions contribute to the stability of the doublet and hence, normal ciliary motility.
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http://dx.doi.org/10.7554/eLife.52760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994238PMC
January 2020

Involvement of Phosphatase and Tensin Homolog in Cyclin-Dependent Kinase 4/6 Inhibitor-Induced Blockade of Glioblastoma.

Front Pharmacol 2019 7;10:1316. Epub 2019 Nov 7.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.

Dysregulation of retinoblastoma (Rb) signaling pathway have been established as a requirement for glioblastoma (GBM) initiation and progression, which suggests that blockade of CDK4/6-Rb signaling axis for GBM treatment. Palbociclib, a selective inhibitor of the cyclin-dependent kinases CDK4/6, has been applied for breast cancer treatment. However, its efficacy against glioblastoma has not been well clarified. Here, effects of CDK4/6 inhibitors on various kinds of GBM cell lines are investigated and the functional mechanisms are identified. Data showed that cells with diverse PTEN status respond to palbociclib differently. Gain-of-function and loss-of-function studies indicated that PTEN enhanced the sensitivity of GBM cells to palbociclib and , which was associated with suppressions of Akt and ERK signaling and independent of Rb signaling inhibition. Hence, our findings support that palbociclib selectively.
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http://dx.doi.org/10.3389/fphar.2019.01316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854038PMC
November 2019

Gold nano-mesh synthesis by continuous-flow X-ray irradiation.

J Synchrotron Radiat 2019 Nov 23;26(Pt 6):1929-1935. Epub 2019 Sep 23.

Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.

X-ray irradiation has been extensively used in recent years as a fabrication step for nanoparticles and nanoparticle systems. A variant of this technique, continuous-flow X-ray irradiation, has recently been developed, and offers three important advantages: precise control of the irradiation dose, elimination of convection effects in the precursor solution, and suitability for large-scale production. Here, the use of this method to fabricate Au nano-meshes of interest as transparent and flexible electrodes for optoelectronics is reported. The study includes extensive characterization of the synthesis parameters and of the product properties, with rather encouraging results.
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http://dx.doi.org/10.1107/S1600577519011834DOI Listing
November 2019
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