Publications by authors named "Shun Wu"

58 Publications

LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.

Aging (Albany NY) 2021 08 10;13(15):19306-19316. Epub 2021 Aug 10.

Department of Breast Surgery, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province 430079, China.

Background: Triple negative breast cancer (TNBC) is a group of highly heterogeneous mixed breast cancer at the level of gene expression profile. Therefore, it is of great clinical significance to explore the molecular mechanism of TNBC and find a targeted therapeutic approach from the molecular level.

Methods: Long non-coding RNA (lncRNA) HAGLR expression level was measured by and qRT-PCR in TNBC tissues and cell lines. EdU, MTT, wound healing and Transwell assays were performed to explore the role of HAGLR on the malignancy of TNBC cells. Luciferase assay was used to clarify the binding between miR-335-3p with HAGLR and WNT2. The tumor formation experiment in nude mice was used to explore the function of HAGLR .

Results: HAGLR was increased in TNBC tissues and cell lines. Silencing of HAGLR inhibited viability, proliferation, migration, and invasion of BT549 cells. Furthermore, HAGLR acted as a sponge of miR-335-3p and inhibited its expression. And miR-335-3p directly targeted WNT2. Functionally, forced expression of miR-335-3p or knockdown of WNT2 removed the promoted effects of lncRNA HAGLR on TNBC development. tumorigenesis experiments indicated HAGLR accelerated tumor growth via miR-335-3p/WNT2 axis.

Conclusion: Our study revealed that HAGLR promoted the growth of TNBC, which was mediated by miR-335-3p/WNT2 axis.
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http://dx.doi.org/10.18632/aging.203272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386551PMC
August 2021

LncRNA BCAR4 expression and predicts the clinical response to neoadjuvant chemotherapy in patients with locally advanced breast cancer.

Cancer Biomark 2021 Jun 19. Epub 2021 Jun 19.

Department of Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Background: Neoadjuvant chemotherapy (NAC) is an important treatment for locally advanced breast cancer (LABC). However, there are no effective biomarkers to predict the efficacy. Therefore, there is an urgent need for new biomarkers to predict the response of LABC to NAC. LncRNA BCAR4 has been detected in a variety of malignant tumor tissues and used as a new biomarker for diagnosis and prognosis. However, LncRNA BCAR4 predicts the response of LABC to NAC is unclear.

Objective: Explore the predictive effect of LncRNA BCAR4 on the efficacy of NAC for LABC in three different evaluation systems.

Methods: First, the TCGA database was used to analyze the expression of LncRNA BCAR4 in 33 kinds of malignant tumors, and further explore its expression in breast cancer and its impact on the survival and prognosis of breast cancer. Furthermore, quantitative methods were used to measure the expression level of LncRNA BCAR4 in cancer tissues of 48 LABC patients, and the correlation between LncRNA BCAR4 and clinicopathological status and response to NAC under the evaluation system of 3, RECIST1.1, Miller-Payne (MP) score and whether it reaches pCR,was analyzed.

Results: TCGA data analysis found that LncRNA is highly expressed in a variety of malignant tumor tissues, including breast cancer. And relatively low expression, the shorter the overall survival time of high expression patients. The high expression of LncRNA BCAR4 is related to the size of the tumor, and there are differences in expression between stage I and other stages, but there is no obvious correlation with the positive lymph node and hormone receptor status. Among the three evaluation systems, only in the RECIST 1.1 evaluation system LncRNA BCAR4 has a predictive effect on NAC for LABC. The expression of LncRNA BCAR4 has no significant correlation with clinical stage, Ki-67% and hormone receptor status, and has no significant correlation with whether patients with locally advanced breast cancer obtain pCR during neoadjuvant chemotherapy.

Conclusion: LncRNA BCAR4 is highly expressed in LABC tissues and may be an effective marker for predicting the efficacy of NAC for LABC.
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http://dx.doi.org/10.3233/CBM-210048DOI Listing
June 2021

Nitrogen-carbon materials base on pyrolytic graphene hydrogel for oxygen reduction.

J Colloid Interface Sci 2021 Nov 8;602:274-281. Epub 2021 Jun 8.

Lab of Advanced Nano-structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China; Chemistry and Chemical Engineering Guangdong Laboratory, Shantou 515031 China. Electronic address:

Hypothesis: Oxygen reduction reaction (ORR) has played a significant role in the utilization of energy nowadays. Nitrogen-doped carbon materials are seen as promising catalysts for ORR, so it is of great significance in studying the functions of different nitrogen moieties.

Experiments: The graphene hydrogel-based nitrogen-arbon materials (GH N-C) were fabricated by first obtaining a gel through hydrothermal treatment using graphene oxide (GO) as precursor, and then calcined in an ammonia atmosphere at different temperatures to form N-doped graphitized materials with divers nitrogen configuration.

Findings: GH N-C materials with tunable nitrogen configuration were synthesized by a two-step method base on graphene hydrogel. Benefiting from the 3D hydrogel structure, rich defects and optimized chemical properties, GH N-C-900 prepared by NH pyrolysis at 900 °C exhibits an excellent electrocatalytic performance toward ORR, with the onset potential of 0.947 ± 0.013 V versus RHE, half-wave potential of 0.830 ± 0.010 V versus RHE, electron transfer number of 3.61-3.99, along as methanol tolerance and superior long-term stability. Comprehensive studies have shown that there is a positive correlation between the total amount of pyrrolic-N and quaternary-N and the catalytic performance of ORR.
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http://dx.doi.org/10.1016/j.jcis.2021.06.036DOI Listing
November 2021

Elucidating the relationship between nutrition indices and coronary artery calcification in patients undergoing maintenance hemodialysis.

Ther Apher Dial 2021 May 26. Epub 2021 May 26.

Department of Nephrology, the Affiliated Huaihai Hospital of Xuzhou Medical University, Xuzhou, China.

Cardiovascular diseases (CVD) are common in maintenance hemodialysis (MHD) patients, and vascular calcification is associated with the incidence of CVD. Malnourished MHD patients are particularly prone to CVD events. Thus far, there is no clear explanation for the relationship of nutrition status with vascular calcification; therefore, we investigated the relationship between malnutrition and vascular calcification. One hundred thirty-one patients underwent laboratory testing, assessment of vascular calcification, modified quantitative subjective global assessment (MQSGA), bioelectrical impedance analysis (BIA), and anthropometric measurements. The patients were divided into two groups based on the presence or absence of coronary artery calcification (CAC), and nutritional statuses were compared between the two groups. The MQSGA score was higher in the CAC group (mean 10.9 ± 1.81) than in the no-CAC group (mean 10.2 ± 1.51); in addition, the mean phase angle (PA) value was significantly lower in the CAC group than in the no-CAC group. Stratification according to CAC score showed that age, Kt/V, incidence of valve calcification, incidence of abdominal aortic calcification, MQSGA score, and blood cell mass were related to the severity of CAC. In addition, quartile analysis revealed that MQSGA score and PA value were related to the incidence and severity of vascular calcification. Binary regression analysis showed that MQSGA score, age, hemoglobin level, and high-density lipoprotein level were independent risk factors for dialysis-related CAC. Patients on MHD who exhibited malnutrition were more likely to have vascular calcification, especially CAC. Namely, the higher the MQSGA score, the lower the PA, and the more likely the occurrence of CAC.
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http://dx.doi.org/10.1111/1744-9987.13693DOI Listing
May 2021

Dissecting the Roles of LncRNAs in the Development of Periventricular White Matter Damage.

Front Genet 2021 30;12:641526. Epub 2021 Apr 30.

Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China.

Long non-coding RNA (LncRNA) has high expression in the brain. Animal studies have shown that lncRNA plays an important role in brain functions and mediates the development of many neurological diseases. However, data on the expression of lncRNAs and the clinical significance in prematurely born infants with diseases such as periventricular white matter damage (PWMD) remains scant. Here, we compared the expression of the lncRNAs in whole blood samples obtained from prematurely born infants with PWMD with samples from prematurely born infants without PWMD. Our data demonstrated differential expression of the lncRNAs between the two groups. Further, we showed that the lncRNAs play important roles in the development of PWMD. Our findings give insights into the functions of the lncRNAs in PWMD and provide evidence for the improvement of diagnostic and treatment strategies in infants with PWMD.
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http://dx.doi.org/10.3389/fgene.2021.641526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120246PMC
April 2021

Risk assessment of vertebral compressive fracture using bone mass index and strength predicted by computed tomography image based finite element analysis.

Clin Biomech (Bristol, Avon) 2021 May 23;85:105365. Epub 2021 Apr 23.

Inazawa Municipal Hospital, Department of Neurosurgery, Inazawa, Aichi 492-8510, Japan.

Background: A main purpose of osteoporosis diagnosis is to evaluate the bone fracture risk. Some bone mass indices evaluated using bone mineral density has been utilized clinically to assess the degree of osteoporosis. On the other hand, Computed tomography image based finite element analysis has been developed to evaluate bone strength of vertebral bodies. The strength of a vertebra is defined as the load at the onset of compressive fracture. The objective of this study was therefore to propose a new feasible method to combine the advantages of the two osteoporotic indices such as the bone mass index and the bone strength.

Methods: Three-dimensional finite element models of 246 vertebral bodies from 88 patients were constructed using the computed tomography images. Finite element analysis was then conducted to evaluate their strength values. The Pearson's correlation analysis was also conducted between the vertebral strength and bone mass indices.

Findings: It was found that relatively weak positive correlations existed between the strength and the bone mass indices. A new assessment method was then proposed by combining the strength and the bone mass index. "high risk zone" corresponding to low strength with normal bone mass was found from the assessment method.

Interpretation: Singe bone mass index cannot predict the fracture risk with high standard. The results of fracture risk assessment conducted by the new method clearly indicated the necessity and effectiveness to take both the strength and the bone mass index into account.
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http://dx.doi.org/10.1016/j.clinbiomech.2021.105365DOI Listing
May 2021

Temperature-independent ultra-sensitive refractive index sensor based on hollow-core silica tubes and tapers.

Opt Express 2021 Mar;29(7):10939-10948

We demonstrate a simple and ultra-sensitive refractive index (RI) sensor using a hollow-core silica tube (HCST) sandwiched between an up-taper and a down-taper in single mode fibers (SMF). According to our theoretical analysis, the interference spectrum comes from a combination of a three-beam multi-mode interference and anti-resonance effects. RI sensing will affect the mode interference. By demodulating the fringe contrast of the interference spectra, an ultrahigh sensitivity of -120.18 dB/RIU is achieved, implying a RI resolution of ∼ 8×10 in the RI range from 1.35 to 1.43. What's more, the sensor has great temperature insensitivity of -0.0085 dB/°C, indicating an extremely low cross sensitivity of 7×10 RIU/°C, which further benefits its practical application. The proposed configuration does not require special fiber or fabrication technique. In addition, the sensor's other merits such as simple and compact structure and ease offabrication offer the potential in biochemical sensing applications.
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http://dx.doi.org/10.1364/OE.422701DOI Listing
March 2021

Do medullary thyroid carcinoma patients with high calcitonin require bilateral neck lymph node clearance? A case report.

World J Clin Cases 2021 Feb;9(6):1343-1352

Department of Thyroid and Breast Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China.

Background: In clinical work, 85%-90% of malignant thyroid diseases are papillary thyroid cancer (PTC); thus, clinicians neglect other types of thyroid cancer, such as medullary thyroid carcinoma (MTC).

Case Summary: We report a 53-year-old female patient with a preoperative calcitonin level of 345 pg/mL. There was no definitive diagnosis of MTC by preoperative fine-needle aspiration cytology or intraoperative frozen pathology, but the presence of PTC and MTC was confirmed by postoperative paraffin pathology. The patient underwent total thyroidectomy and bilateral central lymph node dissection. Close follow-up at 1.5 years after surgery revealed no signs of recurrence or metastasis.

Conclusion: The issue in clinical work-up regarding types of thyroid cancer provides a novel and challenging idea for the surgical treatment of MTC. In the absence of central lymph node metastasis, it is worth addressing whether patients with high calcitonin can undergo total thyroidectomy and bilateral central lymph node dissection without bilateral lateral neck lymph node dissection.
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http://dx.doi.org/10.12998/wjcc.v9.i6.1343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896685PMC
February 2021

Tumor necrosis factor superfamily 14 is critical for the development of renal fibrosis.

Aging (Albany NY) 2020 11 24;12(24):25469-25486. Epub 2020 Nov 24.

Department of Nephrology, The First Affiliated Hospital of Army Medical University (Third Military Medical University), Shapingba 400038, Chongqing, China.

Objective: Tumor necrosis factor superfamily protein 14 (TNFSF14) was recently identified as a risk factor in some fibrosis diseases. However, the role of TNFSF14 in renal fibrosis pathogenesis remains unknown.

Results: It was found that TNFSF14 levels were significantly increased both in UUO-induced renal fibrotic mice and in patients with fibrotic nephropathy, compared with those in controls. Accordingly, deficiency led to a marked reduction in renal fibrosis lesions and inflammatory cytokines expression in the UUO mice. Furthermore, the levels of Sphk1, a critical molecule that causes fibrotic nephropathy, were remarkably reduced in KO mice with UUO surgery. In vitro recombinant TNFSF14 administration markedly up-regulated the expression of Sphk1 of primary mouse renal tubular epithelial cells (mTECs).

Conclusion: TNFSF14 is a novel pro-fibrotic factor of renal fibrosis, for which TNFSF14 up-regulates Sphk1 expression, which may be the underlying mechanism of TNFSF14-mediated renal fibrosis.

Methods: We investigated the effect of TNFSF14 on renal fibrosis and the relationship between TNFSF14 and pro-fibrotic factor sphingosine kinase 1 (Sphk1) by using the unilateral urethral obstruction (UUO)-induced mice renal fibrosis as a model and the specimen of patients with fibrosis nephropathy, by Masson trichrome staining, immunohistochemistry, qRT-PCR, and western blot analysis.
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http://dx.doi.org/10.18632/aging.104151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803499PMC
November 2020

LIGHT deficiency aggravates cisplatin-induced acute kidney injury by upregulating mitochondrial apoptosis.

Int Immunopharmacol 2020 Dec 9;89(Pt A):106999. Epub 2020 Oct 9.

Department of Nephrology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. Electronic address:

Cisplatin is widely used as a chemotherapeutic agent for treating patients with solid tumors. The most common side effect of cisplatin treatment is nephrotoxicity. Recent studies have shown that mitochondrial apoptotic pathways are involved in cisplatin-induced acute kidney injury (Cis-AKI). LIGHT, the 14th member of the tumor necrosis factor superfamily (TNFSF14), was found to induce apoptosis of certain types of tumor cells. So far, a link between LIGHT and Cis-AKI has not been reported. In this study, we observed that expression of LIGHT and its receptors HVEM and LTβR was increased in kidney tissues of mice after cisplatin treatment. LIGHT deficiency aggravated kidney injury, as evidenced by more severe tubular injury; remarkably increased levels of serum creatinine (Scr), blood urea nitrogen (BUN), and both kidney injury molecule-1 (KIM-1) and inflammatory cytokine mRNAs in renal tissues. Moreover, in the renal tissues of LIGHT KO mice, cisplatin-induced mitochondrion injury and the levels of the pro-apoptotic molecules Bax, Cytochrome C (Cyt C), cleaved caspase-3, and cleaved caspase-9 were dramatically increased; in contrast, the expression of anti-apoptotic molecule Bcl-2 was markedly reduced, compared to those in WT mice, suggesting that LIGHT deficiency accelerated cisplatin-induced mitochondrial apoptosis of renal tubular cells in these mice. Accordingly, treatment with recombinant human LIGHT (rLIGHT) was shown to alleviate cisplatin-induced kidney injury in vivo. Similar results were observed after the human renal tubular epithelial cell line HK-2 cells exposure to rLIGHT stimulation, evidenced by the reduction in the mitochondrion dysfunction (as confirmed by the significant reduced oxidative stress and membrane potential changes) and in the percentage of cells apoptosis. While blocking LIGHT with the soluble fusion protein LTβR-Ig or HVEM-Ig accelerated the HK-2 cells apoptosis. In conclusion, LIGHT deficiency aggravates Cis-AKI by promoting mitochondrial apoptosis pathways.
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http://dx.doi.org/10.1016/j.intimp.2020.106999DOI Listing
December 2020

LIGHT aggravates sepsis-associated acute kidney injury via TLR4-MyD88-NF-κB pathway.

J Cell Mol Med 2020 10 3;24(20):11936-11948. Epub 2020 Sep 3.

Department of Nephrology, Southwest Hospital, Army Medical University, Chongqing, China.

Sepsis-associated acute kidney injury (SA-AKI) is a common clinical critical care syndrome. It has received increasing attention due to its high morbidity and mortality; however, its pathophysiological mechanisms remain elusive. LIGHT, the 14th member of the tumour necrosis factor (TNF) superfamily and a bidirectional immunoregulatory molecule that regulates inflammation, plays a pivotal role in disease pathogenesis. In this study, mice with an intraperitoneal injection of LPS and HK-2 cells challenged with LPS were employed as a model of SA-AKI in vivo and in vitro, respectively. LIGHT deficiency notably attenuated kidney injury in pathological damage and renal function and markedly mitigated the inflammatory reaction by decreasing inflammatory mediator production and inflammatory cell infiltration in vivo. The TLR4-Myd88-NF-κB signalling pathway in the kidney of LIGHT knockout mice was dramatically down-regulated compared to the controls. Recombinant human LIGHT aggravated LPS-treated HK-2 cell injury by up-regulating the expression of the TLR4-Myd88-NF-κB signalling pathway and inflammation levels. TAK 242 (a selective TLR4 inhibitor) reduced this trend to some extent. In addition, blocking LIGHT with soluble receptor fusion proteins HVEM-Fc or LTβR-Fc in mice attenuated renal dysfunction and pathological damage in SA-AKI. Our findings indicate that LIGHT aggravates inflammation and promotes kidney damage in LPS-induced SA-AKI via the TLR4-Myd88-NF-κB signalling pathway, which provide potential strategies for the treatment of SA-AKI.
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http://dx.doi.org/10.1111/jcmm.15815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579683PMC
October 2020

Fibrinogen-like protein 2 deficiency aggravates renal fibrosis by facilitating macrophage polarization.

Biomed Pharmacother 2020 Oct 11;130:110468. Epub 2020 Aug 11.

Department of Immunology, Basic Medicine College, Army Medical University (Third Military Medical University), Chongqing 400038, China. Electronic address:

Renal fibrosis has no effective target for its prevention or reversal. Fibinogen-like protein 2 (Fgl2) is a novel prothrombinase exhibiting coagulation activity and immunomodulatory effects. Although Fgl2 is known to play a vital role in the development of liver and interstitial fibrosis, its function in renal fibrosis remains unclear. In this study, Fgl2 expression was found to be markedly increased in kidney tissues from mice with unilateral ureteral obstruction (UUO)-induced renal fibrosis and patients with chronic kidney disease. However, Fgl2 deficiency aggravated UUO-induced renal fibrosis, as evidenced by the significantly increasing collagen I, fibronectin, and α-SMA expression, extracellular matrix deposition, and profibrotic factor (TGF-β1) secretion. Administration of rmFgl2 (recombinant mouse Fgl2) significantly alleviated UUO-induced renal fibrosis in mice, suggesting that the increased fibrosis can be reversed by supplementing rmFgl2. Although there was no difference in the percentages of total macrophages between Fgl2 and Fgl2 mice, Fgl2 deficiency remarkably facilitated M2 macrophage polarization and accelerated M1 macrophage polarization to a low degree, during UUO-induced renal fibrosis development in mice. Similar results were observed when Fgl2 and Fgl2 mice bone marrow-derived macrophages were treated for M1 or M2 polarization. Moreover, Fgl2 deficiency significantly increased the phosphorylation of STAT6, a critical mediator of M2 polarization, in both UUO-induced fibrotic kidney tissues and bone marrow-derived M2 macrophages. In conclusion, the aggravation of renal fibrosis by Fgl2 deficiency is facilitated by the p-STAT6-dependent upregulation of macrophage polarization, especially of M2.
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http://dx.doi.org/10.1016/j.biopha.2020.110468DOI Listing
October 2020

Reversible posterior leukoencephalopathy syndrome due to adrenal pheochromocytoma: A case report and literature review.

Medicine (Baltimore) 2020 Jul;99(28):e20918

Department of Nephrology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Rationale: Reversible posterior leukoencephalopathy syndrome (RPLS) is a rare neuropathic syndrome with typical clinical and radiological features. There are large amounts of risk factors resulting in RPLS, those including hypertension, eclampsia, neoplasia treatment, renal failure, systemic infections, chemotherapy, and immunosuppressive therapy after organ transplantation.

Patient Concerns: A 27-year-old male patient was admitted for a 2-week history of paroxysmal tic of limbs along with consciousness disorder. Blood pressure elevation was discovered for the first time on admission, and the highest record was 210/150 mmHg during hospitalization. Neurological examinations were positive among mental state, speech, reaction and pathological reflex. The computed tomography scan of the abdomen demonstrated a mass derived from right adrenal gland. The magnetic resonance imaging of the brain showed reversible lesions in the centrum ovale, paraventricular, area and corpus callosum.

Diagnoses: After control of blood pressure and rationally preoperative preparation, the mass was radically resected and verified to be pheochromocytoma by postoperative pathologic findings. He was diagnosed as having RPLS due to adrenal pheochromocytoma.

Interventions: The right adrenal gland mass was completely removed after 2 weeks of α-blockers and β-blockers to treat hypertension.

Outcomes: One week after surgery, the cerebral lesions of RPLS gradually faded and the blood pressure was easy to control well.

Lessons: A few case reports of RPLS related to pheochromocytomas had been documented in the literature. Therefore, we believe that pheochromocytomas may be a potential risk factor of RPLS. If patients receive timely diagnosis and treatment, it can often lead to a favorable prognosis.
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http://dx.doi.org/10.1097/MD.0000000000020918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360299PMC
July 2020

C5a/C5aR1 mediates IMQ-induced psoriasiform skin inflammation by promoting IL-17A production from γδ-T cells.

FASEB J 2020 08 18;34(8):10590-10604. Epub 2020 Jun 18.

Department of Rheumatism and Immunology, Daping Hospital, Army Medical University, Chongqing, China.

Psoriasis is a chronic relapsing inflammatory skin disease, affecting up to 3% of the global population. Accumulating evidence suggests that the complement system is involved in its pathogenesis. Our previous study revealed that the C5a/C5aR1 pathway is crucial for disease development. However, the underlying mechanisms remain largely unknown. To explore potential mechanisms, psoriatic skin lesions and histological changes were assessed following imiquimod (IMQ) cream treatment. Inflammatory cytokine expression was tested by real-time RT-PCR. Immunohistochemistry and flow cytometry were used to identify inflammatory cell infiltration and interleukin (IL-17A) IL-17A expression. A C5aR1 antagonist (C5aR1a) and PI3K inhibitor (wortmannin) were used for blocking experiments (both in vivo and in vitro) to explore the mechanism. C5a/C5aR1-pathway inhibition significantly attenuated psoriasis-like skin lesions with decreased epidermal hyperplasia, downregulated type 17-related inflammatory gene expression, and reduced IL-17A-producing γδ-T cell responses. Mechanistically, C5a/C5aR1 promoted the latter phenotype via PI3K-Akt signaling. Consistently, C5aR1 deficiency clearly ameliorated IMQ-induced chronic psoriasiform dermatitis, with a significant decrease in IL-17A expression. Finally, blocking C5aR1 signaling further decreased psoriasiform skin inflammation in IL-17-deficient mice. Results suggest that C5a/C5aR1 mediates experimental psoriasis and skin inflammation by upregulating IL-17A expression from γδ-T cells. Blocking C5a/C5aR1/IL-17A axis is expected to be a promising strategy for psoriasis treatment.
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http://dx.doi.org/10.1096/fj.202000384RDOI Listing
August 2020

Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice.

Int Immunopharmacol 2020 Aug 11;85:106692. Epub 2020 Jun 11.

Department of Immunology, Army Medical University, Chongqing 400038, China. Electronic address:

Complement component 3 (C3), a pivotal molecule in the complement system, is an essential immune mediator in various diseases, including psoriasis. However, the mechanistic role of C3 in psoriasis pathology and development remains elusive. Here, we showed that C3 deficiency dramatically augmented imiquimod-induced psoriasis-like skin inflammation, characterized by greater epidermal hyperplasia, inflammatory cell infiltration, and inflammatory gene expression than those in wild-type counterparts. In addition, C3 deficiency promoted imiquimod-induced skin cell apoptosis and supported greater proportions of IFN-γ T cells in the inflamed tissues. Accordingly, C3 supplement in the C3 deficient mice reduced skin inflammation and cells apoptosis. Moreover, blocking apoptosis with Z-VAD-FMK, a broad caspase inhibitor, markedly attenuated imiquimod-induced psoriasis-like skin inflammation and IFN-γ T cell responses in C3-deficient mice. Collectively, our results suggest that C3 prevents imiquimod-induced psoriasis-like skin inflammation by inhibiting apoptosis.
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http://dx.doi.org/10.1016/j.intimp.2020.106692DOI Listing
August 2020

Circular RNA expression alteration in whole blood of premature infants with periventricular white matter damage.

Genomics 2020 07 1;112(4):2875-2885. Epub 2020 Apr 1.

Department of Pediatrics, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu, China. Electronic address:

Circular RNAs (circRNAs) are evolutionarily conserved and tissue-specific types of non-coding RNA and can serve as potential diagnostic biomarkers for disease. However, the clinical significance and levels of expression of circRNAs for whole blood samples of prematurely born infants afflicted by diseases such as periventricular white matter damage (PWMD) are largely unknown. Therefore, we sought to identify measures of expression of circRNAs in whole blood samples obtained from prematurely born infants afflicted by PWMD and comparatively in samples from prematurely born infants without PWMD. We found the expression levels of circRNAs which from premature with PWMD has changed. Further analysis suggests that these circRNAs have important roles in PWMD. This study can improve the understanding for the potential of the circRNAs to serve as biomarkers in PWMD. Moreover, these circRNAs may provide evidence for improving diagnosis and treatment for infants afflicted by PWMD, and merits continued research.
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http://dx.doi.org/10.1016/j.ygeno.2020.03.027DOI Listing
July 2020

An electrochemical strategy for tetracycline detection coupled triple helix aptamer probe with catalyzed hairpin assembly signal amplification.

Biosens Bioelectron 2019 Oct 21;143:111613. Epub 2019 Aug 21.

Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China. Electronic address:

Incorporating elements of triple-helix aptamer probes (TAP), catalyzed hairpin assembly (CHA) signal amplification and host-guest recognition, a novel "signal-on" sensing strategy for sensitive electrochemical quantification of tetracycline (TC) was reported unprecedentedly. TAP was formed involving an aptamer loop, two-segment stems and a triplex oligonucleotide serving as trigger probe. Then, the trigger probe would be released from TAP once the target presented due to the conformational variation of TAP induced by aptamer binding event, sparking off the upcoming CHA amplification reaction, in which two coexisting DNA hairpins (H1 and H2 both modified with the electroactive molecules) would hybridize into plentiful H1-H2 double helices. Afterwards, the Exonuclease III was added, demolishing double helices and simultaneously releasing plentiful electroactive molecules which were capable of diffusing onto the electrode surface under the assistance of β-cyclodextrin due to host-guest recognition, where appreciable signals were enriched and generated. As thus, considerably slight amounts of targets though, emitted trigger probes, yet efficiently engining spectacular CHA cycles of reactions through which amplified signals were yielded, and in turn progressively enabling the sensitive target detection done. Under optimal conditions, the growing signal stayed a linear relation along with the logarithm of the target concentrations ranging from 0.2 nM to 100 nM, the detection limit reaching as low as 0.13 nM. This approach was desirable regarding to sensitivity, detection limit and range, prospectively rendering a service for diverse targets detection by easily replacing the matched aptamer loop of TAP.
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http://dx.doi.org/10.1016/j.bios.2019.111613DOI Listing
October 2019

C5a/C5aR1 Pathway Is Critical for the Pathogenesis of Psoriasis.

Front Immunol 2019 7;10:1866. Epub 2019 Aug 7.

Department of Immunology, Army Medical University (Third Military Medical University), Chongqing, China.

Psoriasis is one of the most common chronic inflammatory skin diseases, affecting ~2% of the population. The lack of characterization of the pathogenesis of psoriasis has hindered efficient clinical treatment of the disease. In our study, we observed that expression of complement component 5a receptor 1(C5aR1) was significantly increased in skin lesions of both imiquimod (IMQ) and IL23-induced psoriatic mice and patients with psoriasis. C5aR1 deficiency or treatment with C5a receptor 1 antagonist (C5aR1a) in mice significantly attenuated psoriasis-like skin lesions and expression of inflammatory cytokines and chemokines. Moreover, C5aR1 deficiency significantly decreased IMQ-induced infiltration of plasmacytoid dendritic cells (pDCs), monocytes and neutrophils in psoriatic skin lesions and functions of pDCs, evidenced by the remarkable reduction in the IMQ-induced production of interferon-α (IFN-α) and tumor necrosis factor α (TNF-α), and FMS-like tyrosine kinase 3 ligand (FLT3L)-dependent pDCs differentiation. Accordingly, treatment with recombinant C5a accelerated pDCs migration and the differentiation of bone marrow cells into pDCs. Furthermore, biopsies of psoriatic patients showed a dramatic increase of C5aR1 pDCs infiltration in psoriatic skin lesions, compared to healthy subjects. Our results provide direct evidence that C5a/C5aR1 signaling plays a critical role in the pathogenesis of psoriasis. Inhibition of C5a/C5aR1 pathway is expected to be beneficial in the treatment of patients with psoriasis.
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http://dx.doi.org/10.3389/fimmu.2019.01866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692472PMC
October 2020

The impact of advice distance on advice taking: Evidence from an ERP study.

Neuropsychologia 2019 06 1;129:56-64. Epub 2019 Mar 1.

School of Psychology, Shandong Normal University, China.

To use ERPs to investigate the impact of advice distance on advice taking in the Judge-Advisor System (JAS) paradigm, behavioural and ERP data were recorded from 20 subjects. The behavioural discrepancies and EEG characteristics of advice taking, as affected by the advice distances, were compared. The results showed that the relationship between the modification rate of a decision maker's initial estimation and the advice distance exhibited an inverse U-shaped curve. During the advice evaluation stage, different advice distances induced the feedback-related negativity (FRN) and subsequent P300 components. The amplitude of the FRN increased as the advice distance increased, while the amplitude of P300 in the zero distance condition was significantly larger than those in the intermediate and far distance conditions. These findings indicate that the advice evaluation process of a decision maker is related to conflict perception, prediction error and motivational or affective significance.
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http://dx.doi.org/10.1016/j.neuropsychologia.2019.02.019DOI Listing
June 2019

Facile combination of beta-cyclodextrin host-guest recognition with exonuclease-assistant signal amplification for sensitive electrochemical assay of ochratoxin A.

Biosens Bioelectron 2019 Jan 11;124-125:82-88. Epub 2018 Oct 11.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, China.

Smartly coupling exonuclease-induced target recycling signal amplifications with β-cyclodextrin host-guest recognition, a novel "signal-on" aptamer sensor for sensitive determination of ochratoxin A (OTA) was proposed for the first time. Firstly, the formation of double-strand DNA (dsDNA) was occurred by hybridizing OTA aptamer with its complementary DNA (cDNA) and as the probe DNA the cDNA at its 3' terminal was labeled with methylene blue (MB). Next, when OTA was present, the aptamer tended to form aptamer-OTA complex with conformation of G-quadruplex instead of aptamer-cDNA duplex, leading to thus the probe DNA separating from dsDNA complex. Then the RecJf exonuclease was added, demolishing partially G-quadruplex structure and releasing a certain number of OTA. Sequentially, those released OTA would continue to react with the rest of aptamer in dsDNA, drawn into development of a new round of G-quadruplex complex, where the target cycling was realized. Meanwhile, as a signal molecule, MB modified on cDNA was liberated along with the cDNA being digested into monoucleotides by RecJf exonuclease, capable of diffusing onto the electrode surface due to host-guest recognition with β-cyclodextrin, whereupon the signal was enriched and yielded. In this way, cycles of target with continuous output of signal indicators were undergone, in which the detection of target was in return fulfilled with signal amplification owing to the joint endeavor of exonuclease and β-cyclodextrin. Under the optimal conditions, the raising signal maintained a linear relation with the logarithm of the target concentrations ranging from 10 pg/mL to 10.0 ng/mL and the detection limit reached as low as 3 pg/mL. This brand-new strategy was simple and low-cost but satisfactory in terms of detection limit, range and sensitivity, in all possibility to be applied extensively for diverse targets detection by easily alternating the corresponding aptamers.
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http://dx.doi.org/10.1016/j.bios.2018.10.007DOI Listing
January 2019

Body Wall Reconstruction for Conjoined Twins: Our Experience and Lessons Learned.

Ann Plast Surg 2018 12;81(6S Suppl 1):S66-S70

From the Department of Plastic Surgery, Union Hospital, and.

Background: Conjoined twinning is a rare congenital malformation, and the resultant huge body wall defects after separation of conjoined twins represent a real challenge to surgeons.

Methods: From 2004 to 2009, authors performed body wall reconstruction for 2 pairs of thoraco-omphalopagus twins and 1 pair of ischiopagus tetrapus twins. Before separation, the techniques of tissue expansion and tractive training were adopted to acquire sufficient skin for final coverage. After separation, the defects of thoracic cage and abdominal myofascial system were repaired with synthetic materials. The closure of the wounds was performed with artificial skin temporarily or by the use of local flaps in 1 stage.

Results: The first pair of thoraco-omphalopagus twins died of circulatory and respiratory failure after emergency surgery, and the other 2 pairs of conjoined twins survived. The second pair of thoraco-omphalopagus twins had wound dehiscence and partial flap necrosis after surgery. The expanded polytetrafluoroethylene mesh in 1 sister of the ischiopagus twins was removed because of local infection 4 years after surgery.

Conclusions: Compressive anatomical understandings and advanced skills in plastic surgery are required for body wall reconstruction in the separation of conjoined twins. In addition, the multidisciplinary team approach has an important role for obtaining satisfactory final surgical outcome.
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http://dx.doi.org/10.1097/SAP.0000000000001609DOI Listing
December 2018

Spinal Muscular Atrophy With Respiratory Distress Type 1-A Child With Atypical Presentation.

Child Neurol Open 2018 19;5:2329048X18769811. Epub 2018 Apr 19.

Department of Paediatrics and Adolescent Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong Kong SAR.

The authors report a child with spinal muscular atrophy with respiratory distress type 1 (SMARD1). She presented atypically with hypothyroidism and heart failure due to septal defects that required early heart surgery and microcephaly in association with cerebral atrophy and thin corpus collosum. The subsequent asymmetrical onset of diaphragmatic paralysis, persistent hypotonia, and generalized muscle weakness led to the suspicion of spinal muscular atrophy with respiratory distress type 1. Sanger sequencing confirmed a compound heterozygous mutation in the Immunoglobulin Mu Binding Protein 2 gene, with a known mutation c.2362C > T (p.Arg788*) and a novel frameshift mutation c.2048delG (p.Gly683A1afs*50). Serial nerve conduction study and electromyography confirmed progressive sensorimotor polyneuropathy and neuronopathy. In summary, this case report describes a child with spinal muscular atrophy with respiratory distress type 1 also with congenital cardiac disease and endocrine dysfunction, expanding the phenotypic spectrum of this condition. A high index of suspicion is needed in diagnosing this rare condition to guide the management and genetic counseling.
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http://dx.doi.org/10.1177/2329048X18769811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946598PMC
April 2018

A novel method for vanadium slag comprehensive utilization to synthesize Zn-Mn ferrite and Fe-V-Cr alloy.

J Hazard Mater 2018 Jul 25;354:99-106. Epub 2018 Apr 25.

Collaborative Innovation Center of Steel Technology, University of Science and Technology Beijing, Beijing 100083, China; State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing 100083, China.

Vanadium slag is a by-product from steelmaking process of vanadium-titanium magnetite, which mainly contains FeO, MnO, VO, and CrO, The elements Fe and Mn are major components of Mn-Zn ferrite. The elements V and Cr are major components of V-Cr alloy. In view of the potential application in these study, a MnZnFeO of high saturation magnetization (Ms = 68.6 emu/g) and low coercivity (Hc = 3.3 Oe) was successfully synthesized from the leaching solutions of vanadium slag by adding appropriate chemical reagents, ZnCl and MnCl·4HO, via roasting at 1300 °C for 1 h. The minor components (CaO and SiO) in the leaching solution of vanadium slag segregated to the grain boundaries resulting in increasing the resistivity of ferrite. The value of DC resistivity of MnZnFeO at 25 °C reached 1230.7Ω m. The residue containing Fe, V and Cr was chlorinated by AlCl and the Fe, V, and Cr ions were released into the NaCl-KCl eutectic. The current-time curve for the electrolysis of molten salt was investigated. Alloy (Fe, V, and Cr) of granular shape was obtained. The residue can be used to produce the mulite. This process provided a new approach to utilize slag from steelmaking.
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http://dx.doi.org/10.1016/j.jhazmat.2018.04.061DOI Listing
July 2018

Modifications of Z-Epicanthoplasty Combined with Double-Eyelid Blepharoplasty in Asians.

Aesthetic Plast Surg 2018 Feb 3;42(1):226-233. Epub 2017 Nov 3.

Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, China.

Background: Epicanthoplasty combined with double-eyelid blepharoplasty is one of the most popular cosmetic procedures in Asians. However, the postoperative medial canthal scar is a concerned issue. Here the authors adopted some modifications of Z-epicanthoplasty to minimize the canthal scar.

Methods: Modifications of Z-epicanthoplasty were mainly in three aspects. First, supratarsal creases were created before epicanthoplasty to prevent the impact of tension variation on epicanthal incisions, caused by elevating and tightening of the upper eyelid skin. Second, one triangle flap was determined according to the transposition of another one in Z-plasty, and hence, it can be guaranteed that the incisions were closed free of tension with more accuracy and less injury to surrounding tissues. Third, linear scar contracture was avoided by adopting discontinuous incisions, and the resulting skin bulge was eliminated by making an additional stitch to anchor the pretarsal skin on the tarsal plate. The follow-up interval was 1 month to 2 years, with a median of 6.5 months.

Results: From January 2012 to December 2015, 237 female and 4 male patients received the surgery. Except five (2.07%) patients, who were not satisfied with the color of incision scars in medial canthal area, none complained about scars 6 months after surgery. The appearance of the supratarsal crease was natural, and the medial canthal scar was inconspicuous in close observation at 2 years.

Conclusion: The modifications of Z-epicanthoplasty are safe and effective. It is potentially helpful to minimize postoperative medial canthal scars.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-017-1001-1DOI Listing
February 2018

LDT classification and therapeutic strategy of congenital body wall defects.

J Plast Reconstr Aesthet Surg 2018 03 19;71(3):384-393. Epub 2017 Sep 19.

Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, PR China. Electronic address:

Background: Repairing body wall defects is a critical step in the treatment of some congenital deformities, and this procedure may need the help from plastic surgeons. Although there are many articles about congenital deformities, body wall defects of these malformations are rarely studied as independent targets.

Methods: In this article, the authors present an LDT classification for congenital body wall defects according to the position of the defects, the tissue layers involved, and the surgical urgency, each of which is represented by letters L, D, and T, respectively. That is, the defects in different areas (L), full-thickness (D), or partial (D, D) defects, defects needing instant repair (T), semi-elective repair (T), or elective repair (T). Based on this classification system, the authors have performed body wall reconstruction on two pairs of thoraco-omphalopagus twins, one pair of ischiopagus tetrapus twins, and an infant and an adult, both of whom were diagnosed with pentalogy of Cantrell associated with ectopia cordis.

Results: Except for one pair of thoraco-omphalopagus twins who died after emergency separation, all the other patients survived. Another pair of thoraco-omphalopagus twins suffered from wound dehiscence and partial flap necrosis, respectively, after surgery. An expanded polytetrafluoroethylene mesh in one sister of the ischiopagus twins was removed because of infection.

Conclusions: LDT classification not only can help doctors categorise different congenital body wall defects rapidly and easily, but can also guide the reconstruction of these defects. It may have clinical value to plastic surgeons to some extent.
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http://dx.doi.org/10.1016/j.bjps.2017.09.002DOI Listing
March 2018

Ultrabroadband tunable OPA design using a spectrally broadened pump source.

Opt Lett 2017 Sep;42(17):3367-3370

A robust optical parametric amplifier (OPA) scheme is proposed in which we have experimentally achieved broadband bandwidth for a collinear OPA design that exhibits good beam quality and spatial distribution using a type-I β barium borate crystal. The applied pump pulses are simultaneously spectrally broadened and temporally stretched in a multi-plate system before being used to amplify the temporally stretched white light. In this case, the phase matching can be obtained for a broad range of wavelengths, and we have managed to achieve bandwidths three times broader than a conventional narrowband pumped collinear OPA. With a bandwidth as broad as 400 nm centered at 1400 nm, as well as a broadband angular dispersion-free idler, the signal bandwidth supports transform-limited pulses as short as 7.5 fs which correspond to sub-two optical cycles for this center wavelength. Furthermore, the system is easily tunable over a 400 nm range of bandwidths starting from 1100 to 1500 nm. The proposed scheme provides a versatile near-infrared/middle-infrared source with a broad bandwidth and fine tuning capability which paves the way for ultrafast spectroscopy and strong field applications.
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http://dx.doi.org/10.1364/OL.42.003367DOI Listing
September 2017

Dual-wavelength highly-sensitive refractive index sensor.

Opt Express 2017 Jun;25(13):14389-14396

We report and demonstrate a highly-sensitive refractive index (RI) sensor based on a linear-cavity dual-wavelength erbium-doped fiber laser (DWEDFL). The optical spectrum of the laser varies as the external environmental RI changes from 1.3 to 1.335. The DWEDFL has a linear-cavity configuration with two fiber Bragg gratings (FBGs) with central wavelengths < 1 nm apart. Since both FBGs share the same EDF gain medium, gain competition occurs in the cavity. Optical loss of one wavelength can be introduced by immersing the sensing component, a 15 mm micro-fiber (MF), in a solution under test. Experimental results demonstrate a high sensitivity of -231.1 dB/RIU (refractive index unit) and 42.6 dB/RIU in the range from 1.300 to 1.335. The relative power change at the two FBG wavelengths reveals a higher sensitivity of -273.7 dB/RIU with better stability due to reduced light source jitter and external perturbation. Due to its high sensitivity and simple structure, the dual wavelengths gain competition RI sensor has potential applications in chemical and biochemical sensing fields.
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http://dx.doi.org/10.1364/OE.25.014389DOI Listing
June 2017

Carrier-envelope offset frequency linewidth narrowing in a Cr:forsterite laser-based frequency comb.

Appl Opt 2016 Dec;55(34):9810-9817

Cr:forsterite laser-based frequency combs are useful for spectroscopic purposes in the near-IR wavelength region. However, self-referenced Cr:forsterite combs tend to exhibit wide carrier-envelope offset frequency (f0) linewidths, which result in broad comb teeth. This can be attributed to significant frequency noise across the comb's spectral bandwidth. We have stabilized a prism-based Cr:forsterite laser comb and observed narrowing of the f0 linewidth from ∼1.4  MHz down to ∼100  kHz by changing only the prism insertion, and to 23 kHz by inserting a knife edge into the intracavity beam while keeping the same prism insertion. The noise dynamics of the Cr:forsterite laser frequency comb are investigated with the goal of explaining this f0 narrowing phenomenon.
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http://dx.doi.org/10.1364/AO.55.009810DOI Listing
December 2016

Electrochemical detection of glutathione by using thymine-rich DNA-gated switch functionalized mesoporous silica nanoparticles.

Biosens Bioelectron 2017 Jan 30;87:459-465. Epub 2016 Aug 30.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.

A novel electrochemical sensor for highly sensitive and selective detection of glutathione (GSH) was developed coupled thymine-Hg-thymine (T-Hg-T) coordination with mesoporous silica nanoparticles (MSN). Hg ions could specifically interact with thymine bases to form strong and stable T-Hg-T complexes. And the electroactive molecules-methylene blue (MB) was sealed in the inner pores of MSN with a unique sequential thymine (T)-rich DNA in the presence of Hg. After the target was added, the formed duplex DNA T-Hg-T complex could deform into single-stranded DNA by a competitive displacement reaction, leading to the release of MB entrapped in the MSN. Thus the target-dependent amount of released MB could be conveniently monitored with an n-dodecanethiol modified screen-printed gold electrode. Using GSH as a model target, the proposed assay showed a wide detection ranging from 1.0nM to 1.0μM with a detection limit of 0.6nM. This strategy was simple and universal for various analytes with different competitive displacement ligands, possessing a great potential for convenient biotools testing and commercial application.
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http://dx.doi.org/10.1016/j.bios.2016.08.102DOI Listing
January 2017

Assessment of the key aroma compounds in rose-based products.

J Food Drug Anal 2016 07 27;24(3):471-476. Epub 2016 Apr 27.

China National Research Institute of Food and Fermentation Industries, Beijing, China.

In this study, headspace solid phase microextraction-gas chromatography-mass spectrometry and GC-olfactometry were used to analyze the key aroma compounds in three types of rose-based products, including low-temperature extracts (LTEs), high-temperature extracts (HTEs), and rose drinks (RDs). In combination with the Guadagni theory, it was confirmed that the key aroma components of LTE were β-phenyl ethyl alcohol, citronellol, geraniol, and eugenol. The main aroma compounds in HTE were β-phenyl ethyl alcohol, citronellol, geraniol, eugenol, linalool, and rose oxide. The four key aroma compounds in RDs were β-phenyl ethyl alcohol, eugenol, geraniol, and linalool.
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http://dx.doi.org/10.1016/j.jfda.2016.02.013DOI Listing
July 2016
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