Publications by authors named "Shujun Zhang"

275 Publications

Carrier type induces anammox biofilm structure and the nitrogen removal pathway: Demonstration in a full-scale partial nitritation/anammox process.

Bioresour Technol 2021 May 4;334:125249. Epub 2021 May 4.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

In this study, two typical carrier types, microporous and macroporous carriers, were collected from a full-scale partial nitritation/anammox reactor for analysis and comparison of the biofilm structure characteristics, performance and removal nitrogen pathway. For microporous carriers, a thicker biofilm (>5 mm) was obtained with higher biomass and abundance of anammox bacteria as well as a higher nitrogen removal efficiency due to the integration of denitrifying and anammox bacteria. In addition, higher microbial community stability can be expected under varying environmental conditions. In comparison, macroporous carrier biofilm exhibited a lower thickness (0.4-2.3 mm) and lower microbial richness, with a strong network correlation among genera. Analysis showed that the mainly positive correlation between anammox bacteria and ammonium oxidizing bacteria, enhancing coupling partial nitritation and anammox. These findings help further our understanding of the mechanisms of anammox biofilm nitrogen removal and provide a baseline for optimization of the design of carrier structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.125249DOI Listing
May 2021

Influence of Estrus on the Milk Characteristics and Mid-Infrared Spectra of Dairy Cows.

Animals (Basel) 2021 Apr 22;11(5). Epub 2021 Apr 22.

Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

Milk produced by dairy cows is a complex combination of many components. However, at present, changes in only a few milk components (e.g., fat, protein, and lactose) during the estrus cycle in dairy cows have been documented. Mid-infrared (MIR) spectroscopy is a worldwide method routinely used for milk analysis, as MIR spectra reflect the global composition of milk. Therefore, this study aimed to investigate the changes in milk MIR spectra and milk production traits (fat, protein, lactose, urea, total solids (TS), and solid not fat (SnF)) due to estrus. Cows that were successfully inseminated, leading to conception, were included. Cows confirmed to be pregnant were considered to be in estrus at the day of insemination (day 0). A general linear mixed model, which included the random effect of cows, the fixed classification effects of parity number, days in relation to estrus, as well as the interaction between parity number and days in relation to estrus, was applied to investigate the changes in milk production traits and 1060 milk infrared wavenumbers, ranging from 925 to 5011 cm, of 371 records from 162 Holstein cows on the days before (day -3, day -2, and day -1) and on the day of estrus (day 0). The days in relation to estrus had a significant effect on fat, protein, urea, TS, and SnF, whose contents increased from day -3 to day 0. Lactose did not seem to be significantly influenced by the occurrence of estrus. The days in relation to estrus had significant effects on the majority of the wavenumbers. Besides, we found that some of the wavenumbers in the water absorption regions were significantly changed on the days before and on the day of estrus. This suggests that these wavenumbers may contain useful information. In conclusion, the changes in the milk composition due to estrus can be observed through the analysis of the milk MIR spectrum. Further analyses are warranted to more deeply explore the potential use of milk MIR spectra in the detection of estrus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani11051200DOI Listing
April 2021

Electroceramics for High-Energy Density Capacitors: Current Status and Future Perspectives.

Chem Rev 2021 Apr 28. Epub 2021 Apr 28.

Department of Materials Science and Engineering, University of Sheffield, Sheffield S1 3JD, U.K.

Materials exhibiting high energy/power density are currently needed to meet the growing demand of portable electronics, electric vehicles and large-scale energy storage devices. The highest energy densities are achieved for fuel cells, batteries, and supercapacitors, but conventional dielectric capacitors are receiving increased attention for pulsed power applications due to their high power density and their fast charge-discharge speed. The key to high energy density in dielectric capacitors is a large maximum but small remanent (zero in the case of linear dielectrics) polarization and a high electric breakdown strength. Polymer dielectric capacitors offer high power/energy density for applications at room temperature, but above 100 °C they are unreliable and suffer from dielectric breakdown. For high-temperature applications, therefore, dielectric ceramics are the only feasible alternative. Lead-based ceramics such as La-doped lead zirconate titanate exhibit good energy storage properties, but their toxicity raises concern over their use in consumer applications, where capacitors are exclusively lead free. Lead-free compositions with superior power density are thus required. In this paper, we introduce the fundamental principles of energy storage in dielectrics. We discuss key factors to improve energy storage properties such as the control of local structure, phase assemblage, dielectric layer thickness, microstructure, conductivity, and electrical homogeneity through the choice of base systems, dopants, and alloying additions, followed by a comprehensive review of the state-of-the-art. Finally, we comment on the future requirements for new materials in high power/energy density capacitor applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.chemrev.0c01264DOI Listing
April 2021

Comparison of the Ability of High and Low Virulence Strains of Non-cytopathic Bovine Viral Diarrhea Virus-1 to Modulate Expression of Interferon Tau Stimulated Genes in Bovine Endometrium.

Front Vet Sci 2021 9;8:659330. Epub 2021 Apr 9.

Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United Kingdom.

Bovine Viral Diarrhea virus (BVDV) is a pestivirus with a single-stranded, positive sense RNA genome. It is endemic in many cattle populations, causing major economic losses in part due to reduced fertility. BVDV exhibits great genetic diversity and is classified as type 1 or 2 (BVDV-1, BVDV-2) with either non-cytopathogenic (ncp) or cytopathogenic (cp) biotypes. Differing strains of ncpBVDV differ in virulence, affecting clinical outcome. BVDV replicates in the reproductive tract, affecting host immunity and embryo survival. This study used an model of primary bovine endometrial cell cultures to compare the effects of two BVDV ncp type 1a strains of differing virulence (termed HO and KY) on endometrial transcription of candidate interferon stimulated genes (ISG) using qPCR. Half the cultures were stimulated with interferon tau (IFNT, the conceptus produced pregnancy recognition factor) in the presence or absence of viral infection. Cultures were replicated on cells from 10 BVDV-free cows. IFNT treatment stimulated transcription of 10 candidate ISGs, whereas both ncpBVDV-1 strains alone inhibited transcription of 8/10 ISGs. In combined BVDV-1+IFNT cultures, the stimulatory effect of IFNT on expression of , and was significantly inhibited by HO, but only , and were decreased by KY. Inhibition by HO was generally greater. The IFNT-induced expression of was, however, increased by HO. These data show that HO, the more virulent ncpBVDV-1 strain, has a greater capacity to inhibit key antiviral pathways. These differences need confirmation at the protein level but may influence immune tolerance of the host. They could also reduce fertility by increasing uterine susceptibility to bacterial infection and disrupting IFNT-mediated pregnancy recognition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2021.659330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062762PMC
April 2021

Effects of parity and days in milk on milk composition in correlation with β-hydroxybutyrate in tropic dairy cows.

Trop Anim Health Prod 2021 Apr 19;53(2):270. Epub 2021 Apr 19.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.

The current study was conducted to evaluate the effect of parity and days in milk on milk yield and milk production traits and their correlation with β-hydroxybutyrate (BHB) concentrations in milk of Chinese tropic Holstein dairy cows which are adapted to a humid subtropical climate in central China. About 3055 milking records of Holstein cows were obtained from three farms in the hot region in the center of China. The records were classified according to parity to 4 categories: first parity, second parity, third parity, and greater than third parity. According to days in milk, there were 4 groups, first group from (1-100 days), second group from (101-200 days), third group from (201-305 days), and fourth group (>305 days). Milk samples collected between April and November 2019 from the three farms were routinely checked for milk components including BHB using mid-infrared spectroscopy a MilkoScan FT+ (Foss, Hillerød, Denmark). Data were analyzed by multivariate analysis of variance (generalized linear model, GLM). Pearson's correlation coefficients were used to measure the correlation between SCC and BHB with milk yield and milk production traits. Results showed the significant effect of parity and days in milk on milk yield and milk production traits. There was a negative effect of parity and days in milk on milk quality, with increasing parity and days in milk being associated with higher somatic cell count (SCC) (P <0.001). Days in milk significantly affected (P=0.001) BHB. It was concluded that with increasing parity and prolonged days in milk, there was a negative effect on milk quality and udder health of the tropic dairy cows in central China. Based on the results of the current study, sampling milk for specific metabolites, somatic cell count, and quality are sufficient to asses herd health.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11250-021-02690-7DOI Listing
April 2021

Genetic Features of Reproductive Traits in Bovine and Buffalo: Lessons From Bovine to Buffalo.

Front Genet 2021 23;12:617128. Epub 2021 Mar 23.

Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

Bovine and buffalo are important livestock species that have contributed to human lives for more than 1000 years. Improving fertility is very important to reduce the cost of production. In the current review, we classified reproductive traits into three categories: ovulation, breeding, and calving related traits. We systematically summarized the heritability estimates, molecular markers, and genomic selection (GS) for reproductive traits of bovine and buffalo. This review aimed to compile the heritability and genome-wide association studies (GWASs) related to reproductive traits in both bovine and buffalos and tried to highlight the possible disciplines which should benefit buffalo breeding. The estimates of heritability of reproductive traits ranged were from 0 to 0.57 and there were wide differences between the populations. For some specific traits, such as age of puberty (AOP) and calving difficulty (CD), the majority beef population presents relatively higher heritability than dairy cattle. Compared to bovine, genetic studies for buffalo reproductive traits are limited for age at first calving and calving interval traits. Several quantitative trait loci (QTLs), candidate genes, and SNPs associated with bovine reproductive traits were screened and identified by candidate gene methods and/or GWASs. The IGF1 and LEP pathways in addition to non-coding RNAs are highlighted due to their crucial relevance with reproductive traits. The distribution of QTLs related to various traits showed a great differences. Few GWAS have been performed so far on buffalo age at first calving, calving interval, and days open traits. In addition, we summarized the GS studies on bovine and buffalo reproductive traits and compared the accuracy between different reports. Taken together, GWAS and candidate gene approaches can help to understand the molecular genetic mechanisms of complex traits. Recently, GS has been used extensively and can be performed on multiple traits to improve the accuracy of prediction even for traits with low heritability, and can be combined with multi-omics for further analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.617128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021858PMC
March 2021

Direct observation of nanoscale dynamics of ferroelectric degradation.

Nat Commun 2021 Apr 7;12(1):2095. Epub 2021 Apr 7.

School of Aerospace, Mechanical and Mechatronic Engineering, The University of Sydney, Sydney, NSW, Australia.

Failure of polarization reversal, i.e., ferroelectric degradation, induced by cyclic electric loadings in ferroelectric materials, has been a long-standing challenge that negatively impacts the application of ferroelectrics in devices where reliability is critical. It is generally believed that space charges or injected charges dominate the ferroelectric degradation. However, the physics behind the phenomenon remains unclear. Here, using in-situ biasing transmission electron microscopy, we discover change of charge distribution in thin ferroelectrics during cyclic electric loadings. Charge accumulation at domain walls is the main reason of the formation of c domains, which are less responsive to the applied electric field. The rapid growth of the frozen c domains leads to the ferroelectric degradation. This finding gives insights into the nature of ferroelectric degradation in nanodevices, and reveals the role of the injected charges in polarization reversal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22355-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027400PMC
April 2021

Role of microRNA-15a-5p/Sox9/NF-κB axis in inflammatory factors and apoptosis of murine nucleus pulposus cells in intervertebral disc degeneration.

Life Sci 2021 Mar 26;277:119408. Epub 2021 Mar 26.

Department of Spine Surgery, Wuxi 9th Affiliated Hospital of Soochow University, Wuxi 214000, Jiangsu, China. Electronic address:

Objective: MicroRNAs are well-established players in post-transcriptional gene modulation. We aim to explore the role of microRNA-15a-5p (miR-15a-5p)/sex determining region Y-box 9 (Sox9)/nuclear factor-κB (NF-κB) axis in inflammation and apoptosis of murine nucleus pulposus cells (NPCs) in intervertebral disc degeneration (IVDD).

Methods: Expression levels of miR-15a-5p and Sox9 in disc tissues from IVDD patients were determined. The IVDD mouse models were established by disc puncture, and the modeled mice were accordingly injected with miR-15a-5p antagomir and/or overexpressed Sox9 plasmid, or their negative controls. Then, the expression of miR-15a-5p, Sox9 and p-p65, pathological changes and the apoptosis of NPCs in IVDD mouse intervertebral disc tissues were measured. The NPCs were isolated and cultured, which were then transfected with miR-15a-5p inhibitor, overexpressed or silenced Sox9 plasmids, or the NCs. Next, the expression of miR-15a-5p and Sox9, apoptosis, proliferation and cell cycle distribution of NPCs, and the contents of inflammatory factors in the NPCs were evaluated.

Results: MiR-15a-5p expression was increased while Sox9 expression was reduced in intervertebral disc tissues from IVDD patients and mice. Mouse NPCs were successfully isolated. The down-regulated miR-15a-5p could elevate Sox9 to activate p-p65 expression, suppress NPC apoptosis and inflammatory factor contents, promote proliferation of NPCs, and arrest the NPCs at S and G2/M phases. However, these effects could be reversed by silencing Sox9.

Conclusion: Reduction of miR-15a-5p elevated Sox9 to inhibit the inflammatory response and apoptosis of NPCs in IVDD mice through the NF-κB pathway. This study may be helpful for IVDD treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119408DOI Listing
March 2021

Large-scale functional network connectivity mediate the associations of gut microbiota with sleep quality and executive functions.

Hum Brain Mapp 2021 Mar 19. Epub 2021 Mar 19.

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Network neuroscience has broadly conceptualized the functions of the brain as complex communication within and between large-scale neural networks. Nevertheless, whether and how the gut microbiota influence functional network connectivity that in turn impact human behaviors has yet to be determined. We collected fecal samples from 157 healthy young adults and used 16S sequencing to assess gut microbial diversity and enterotypes. Large-scale inter- and intranetwork functional connectivity was measured using a combination of resting-state functional MRI data and independent component analysis. Sleep quality and core executive functions were also evaluated. Then, we tested for potential associations between gut microbiota, functional network connectivity and behaviors. We found significant associations of gut microbial diversity with internetwork functional connectivity between the executive control, default mode and sensorimotor systems, and intranetwork connectivity of the executive control system. Moreover, some internetwork functional connectivity mediated the relations of microbial diversity with sleep quality, working memory, and attention. In addition, there was a significant effect of enterotypes on intranetwork connectivity of the executive control system, which could mediate the link between enterotypes and executive function. Our findings not only may expand existing biological knowledge of the gut microbiota-brain-behavior relationships from the perspective of large-scale functional network organization, but also may ultimately inform a translational conceptualization of how to improve sleep quality and executive functions through the regulation of gut microbiota.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/hbm.25419DOI Listing
March 2021

Associations of Serum Liver Function Markers With Brain Structure, Function, and Perfusion in Healthy Young Adults.

Front Neurol 2021 25;12:606094. Epub 2021 Feb 25.

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Previous neuroimaging studies have demonstrated brain abnormalities in patients with hepatic diseases. However, the identified liver-brain associations are largely limited to disease-affected populations, and the nature and extent of such relations in healthy subjects remain unclear. We hypothesized that serum liver function markers within a normal level would affect brain properties. One hundred fifty-seven healthy young adults underwent structural, resting-state functional, and arterial spin labeling MRI scans. Gray matter volume (GMV), regional homogeneity (ReHo), and cerebral blood flow (CBF) analyses were performed to assess brain structure, function, and perfusion, respectively. Peripheral venous blood samples were collected to measure serum liver function markers. Correlation analyses were conducted to test potential associations between liver function markers and brain imaging parameters. First, serum proteins showed relations to brain structure characterized by higher albumin associated with increased GMV in the parahippocampal gyrus and amygdala and lower globulin and a higher albumin/globulin ratio with increased GMV in the olfactory cortex and parahippocampal gyrus. Second, serum bilirubin was linked to brain function characterized by higher bilirubin associated with increased ReHo in the precuneus, middle cingulate gyrus, inferior parietal lobule, and supramarginal gyrus and decreased ReHo in the caudate nucleus. Third, serum alanine transaminase (ALT) was related to brain perfusion characterized by higher ALT associated with increased CBF in the superior frontal gyrus and decreased CBF in the middle occipital gyrus, angular gyrus, precuneus, and middle temporal gyrus. More importantly, we found that CBF in the superior frontal gyrus was a significant mediator of the association between serum ALT level and working memory performance. These findings may not only expand existing knowledge about the relationship between the liver and the brain but also have clinical implications for studying brain impairments secondary to liver diseases as well as providing potential neural targets for their diagnosis and treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.606094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947675PMC
February 2021

Toroidal polar topology in strained ferroelectric polymer.

Science 2021 03;371(6533):1050-1056

State Key Lab of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Polar topological texture has become an emerging research field for exotic phenomena and potential applications in reconfigurable electronic devices. We report toroidal topological texture self-organized in a ferroelectric polymer, poly(vinylidene fluoride--trifluoroethylene) [P(VDF-TrFE)], that exhibits concentric topology with anticoupled chiral domains. The interplay among the elastic, electric, and gradient energies results in continuous rotation and toroidal assembly of the polarization perpendicular to polymer chains, whereas relaxor behavior is induced along polymer chains. Such toroidal polar topology gives rise to periodic absorption of polarized far-infrared (FIR) waves, enabling the manipulation of the terahertz wave on a mesoscopic scale. Our observations should inform design principles for flexible ferroic materials toward complex topologies and provide opportunities for multistimuli conversions in flexible electronics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.abc4727DOI Listing
March 2021

Textured ferroelectric ceramics with high electromechanical coupling factors over a broad temperature range.

Nat Commun 2021 Mar 3;12(1):1414. Epub 2021 Mar 3.

Electronic Materials Research Laboratory (Key Lab of Education Ministry), State Key Laboratory for Mechanical Behavior of Materials and School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an, China.

The figure-of-merits of ferroelectrics for transducer applications are their electromechanical coupling factor and the operable temperature range. Relaxor-PbTiO ferroelectric crystals show a much improved electromechanical coupling factor k (88~93%) compared to their ceramic counterparts (65~78%) by taking advantage of the strong anisotropy of crystals. However, only a few relaxor-PbTiO systems, for example Pb(InNb)O-Pb(MgNb)O-PbTiO, can be grown into single crystals, whose operable temperature range is limited by their rhombohedral-tetragonal phase transition temperatures (T: 60~120 °C). Here, we develop a templated grain-growth approach to fabricate <001>-textured Pb(InNb)O-Pb(ScNb)O-PbTiO (PIN-PSN-PT) ceramics that contain a large amount of the refractory component ScO, which has the ability to increase the T of the system. The high k of 85~89% and the greatly increased T of 160~200 °C are simultaneously achieved in the textured PIN-PSN-PT ceramics. The above merits will make textured PIN-PSN-PT ceramics an alternative to single crystals, benefiting the development of numerous advanced piezoelectric devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21673-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977148PMC
March 2021

Hydrogen Generation and Degradation of Organic Dyes by New Piezocatalytic 0.7BiFeO-0.3BaTiO Nanoparticles with Proper Band Alignment.

ACS Appl Mater Interfaces 2021 Mar 26;13(9):11050-11057. Epub 2021 Feb 26.

Institute for Superconducting and Electronic Materials, Australian Institute for Innovative Materials, University of Wollongong, North Wollongong, New South Wales 2500, Australia.

Piezoelectric materials have recently demonstrated their potential applications in clean energy exploration and environmental remediation through triggering a number of catalytic reactions by harvesting waste vibrational energy in the environment. In this work, unique lead-free 0.7BiFeO-0.3BaTiO (BF-BT) nanoparticles with tuned band structure were synthesized by the hydrothermal method for use as piezoelectric catalysts to generate hydrogen by splitting water; a high production rate of 1.322 mmol/g was achieved in 1 h, which is 10 times higher than the production rate of pure BiFeO. Of particular interest, BF-BT particles attached to nickel mesh have the ability to degrade rhodamine B in flowing water, demonstrating their potential to treat polluted water by anchoring BF-BT in drains. Finally, we propose novel insight on the piezocatalytic mechanism, which is based on the internal electric field (the sum of the depolarization field and the screening charge field) that drives electron/hole separation and movement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c01407DOI Listing
March 2021

Ginsenoside Rg1 alleviates acute liver injury through the induction of autophagy and suppressing NF-κB/NLRP3 inflammasome signaling pathway.

Int J Med Sci 2021 23;18(6):1382-1389. Epub 2021 Jan 23.

Clinical Molecular Medicine Testing Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Severe hepatitis is a common cause of chronic or acute liver disease and autophagy might play an important role in cellular response to inflammation and injury. It has been reported that Ginsenoside-Rg1 (G-Rg1) has strong hepatoprotective effects for acute liver injury, but its protective mechanisms have not yet been elucidated. This study aims to explore the detailed molecular mechanisms of G-Rg1 on acute liver injury via autophagy. The role of G-Rg1 by autophagic induction was studied in the mouse model of acute liver injury which induced by carbon tetrachloride (CCl4). Liver function, inflammatory reaction and apoptosis were detected when autophagy has been inhibited by 3-MA or stimulated by RPA. MCC950 and ATP were applied to investigate the role of NLRP3 inflammasome in acute liver injury. The differential expression of NF-κB, NLRP3 inflammasome, caspase 1, caspase 3, IL-1β, IL-18, LC3-I, LC3-II, Beclin-1, PINK1 and Parkin have been detected by the quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. G-Rg1 could decrease ALT, AST, TNF-α, IL-1β and IL-6 in mice with CCl4-induced acute liver injury. The change of autophagy and apoptosis after the treatment of 3-MA or RPA demonstrated that the autophagy played a key role in the protective effect of G-Rg1 in acute liver injury. The enhancement of G-Rg1 promoted-autophagy resulted in the significant decrease in NF-κB, NLRP3 inflammasome, caspase 1, caspase 3, IL-1β and IL-18, which suggesting that NF-κB/NLRP3 inflammasome signaling pathway was associated with the autophagy induced by G-Rg1 in acute liver injury. G-Rg1 ameliorated acute liver injury via the autophagy, which may be related to NF-κB/NLRP3 inflammasome signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijms.50919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893561PMC
January 2021

Postpartum Uterine Involution and Embryonic Development Pattern in Chinese Holstein Dairy Cows.

Front Vet Sci 2020 22;7:604729. Epub 2021 Jan 22.

Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China.

Understanding the postpartum uterine involution pattern and embryonic development could facilitate bovine reproduction management, improve reproductive efficiency, and diagnosis of the reproductive disorder, which would contribute to the success of the dairy business. This study aimed to investigate postpartum uterine involution and embryonic developmental patterns or postconceptional marks of embryonic fetal development in Chinese Holstein dairy cows using B-mode ultrasonography. The results revealed a significant decline in the involution period with an increase of parity and age. The uterine involution period was shorter in multiparous cows when compared with cows with lower parities. Consistently, cows over 4 years old recovered faster than younger cows (2 or 3 years). Besides, the elder cows (over 4 years) had a relatively larger size of resumed cervix uteri and horns. Postpartum uterine involution pattern analysis revealed that the reproductive tract recovered very fast during the first 16 days postpartum for all the parity. Results of postconceptional marks of embryo development revealed a slow increase in diameter of the gravid uterine horn and crown-rump length (CRL) before day 60. In contrast, this increase was dramatic and rapid after the 60th day. We also established two models to estimate gestational age based on gravid uterine horn diameter or CRL. A formula was established to determine the gravid uterine horn size during postconceptional on day 30th-day 90th ( = 0.8714, < 0.01). In addition, a significant positive correlation between CRL and gestational age ( = 0.98151, < 0.01) was built. In conclusion, these results illustrated that parity and calving age had significant effects on uterine involution in Chinese Holstein cows. Crown-rump length and gravid uterine horn diameter are both efficient for evaluating the embryo growth. These current findings broaden the understanding of basic reproductive pattern in Chinese Holstein cows and could benefit bovine reproductive management primarily in postpartum and early pregnant cows to reduce the calving interval and avoid periparturient metabolic diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2020.604729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874089PMC
January 2021

The mechanism for the enhanced piezoelectricity in multi-elements doped (K,Na)NbO ceramics.

Nat Commun 2021 Feb 9;12(1):881. Epub 2021 Feb 9.

Institute for Superconducting and Electronic Materials, Australian Institute for Innovative Materials, University of Wollongong, Wollongong, NSW, Australia.

(K,Na)NbO based ceramics are considered to be one of the most promising lead-free ferroelectrics replacing Pb(Zr,Ti)O. Despite extensive studies over the last two decades, the mechanism for the enhanced piezoelectricity in multi-elements doped (K,Na)NbO ceramics has not been fully understood. Here, we combine temperature-dependent synchrotron x-ray diffraction and property measurements, atomic-scale scanning transmission electron microscopy, and first-principle and phase-field calculations to establish the dopant-structure-property relationship for multi-elements doped (K,Na)NbO ceramics. Our results indicate that the dopants induced tetragonal phase and the accompanying high-density nanoscale heterostructures with low-angle polar vectors are responsible for the high dielectric and piezoelectric properties. This work explains the mechanism of the high piezoelectricity recently achieved in (K,Na)NbO ceramics and provides guidance for the design of high-performance ferroelectric ceramics, which is expected to benefit numerous functional materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-21202-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873261PMC
February 2021

Hyperosmolarity Deserves More Attention in Critically Ill COVID-19 Patients with Diabetes: A Cohort-Based Study.

Diabetes Metab Syndr Obes 2021 7;14:47-58. Epub 2021 Jan 7.

Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Purpose: Recently, a cluster of pneumonia caused by SARS-CoV-2 were identified in Wuhan and spread throughout the world. More information about risk factors for mortality of critically ill patients infected with SARS-CoV-2 remain to be evaluated.

Methods: We included adult patients confirmed with SARS-CoV-2 infection who were critically ill and admitted to the intensive care unit (ICU) of Tongji Hospital in Wuhan from Feb 4, 2020 to Feb 20, 2020. Data were collected and compared between patients who died and improved. Logistic regression was used to explore the risk factors for death of SARS-CoV-2-infected critically ill patients.

Results: A total of 160 critically ill patients with SARS-CoV-2 infection were included, of which 146 patients with appeared outcomes were included into the final analysis. The random blood glucose, serum sodium and effective plasma osmolarity were higher in deceased patients, especially in patients with diabetes. There were 7 patients with diabetes with hyperosmolar status and all of them were deceased. Multivariable regression revealed that older age (odds ratio 4.28, 95% CI 1.01-18.20; p = 0.049), higher C-reactive protein (odds ratio 1.01, 1.00-1.03; p = 0.024), higher interleukin-6 (odds ratio 1.01, 1.00-1.03; p = 0.0323), and d-dimer greater than 1 μg/mL (odds ratio 1.10, 1.01-1.20; p = 0.032) at admission were associated with increased odds of death.

Conclusion: In conclusion, hyperosmolarity needs more attention and may contribute to mortality in critically ill patients with COVID-19, especially in those with diabetes. Older age, inflammatory response, and thrombosis may be risk factors for death of critically ill patients with SARS-CoV-2 infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S284148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800461PMC
January 2021

Rational Design and Synthesis of Ultra-Thin β-Ni(OH) Nanoplates for High Performance All-Solid-State Flexible Supercapacitors.

Front Chem 2020 1;8:602322. Epub 2020 Dec 1.

Hubei Key Laboratory of Ferro- and Piezoelectric Materials and Devices, Faculty of Physics and Electronic Science, Hubei University, Wuhan, China.

The all-solid-state flexible supercapacitor (AFSC), one of the most flourishing energy storage devices for portable and wearable electronics, attracts substantial attentions due to their high flexibility, compact size, improved safety, and environmental friendliness. Nevertheless, the current AFSCs usually show low energy density, which extremely hinders their practical applications. Herein, ultra-thin β-Ni(OH) nanoplates with thickness of 2.4 ± 0.2 nm are grown uniformly on Ni foam by one step hydrothermal treatment. Thanks to the ultra-thin nanostructure, β-Ni(OH) nanoplates shows a specific capacitance of 1,452 F g at the scan rate of 3 mV s. In addition, the assembled asymmetric AFSC [Ni(OH)//Activated carbon] shows a specific capacitance of 198 F g. It is worth noting that the energy density of the AFSC can reach 62 Wh kg while keeping a high power density of 1.5 kW kg. Furthermore, the fabricated AFSCs exhibit satisfied fatigue behavior and excellent flexibility, and about 82 and 86% of the capacities were retained after 5,000 cycles and folding over 1,500 times, respectively. Two AFSC in series connection can drive the electronic watch and to run stably for 10 min under the bending conditions, showing a great potential for powering portable and wearable electronic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2020.602322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733587PMC
December 2020

Changes in oxidation-antioxidation function on the thymus of chickens infected with reticuloendotheliosis virus.

BMC Vet Res 2020 Dec 11;16(1):483. Epub 2020 Dec 11.

College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China.

Background: Reticuloendotheliosis virus (REV) is a retrovirus that causes severe immunosuppression in poultry. Animals grow slowly under conditions of oxidative stress. In addition, long-term oxidative stress can impair immune function, as well as accelerate aging and death. This study aimed to elucidate the pathogenesis of REV from the perspective of changes in oxidative-antioxidative function following REV infection.

Methods: A total of 80 one-day-old specific pathogen free (SPF) chickens were randomly divided into a control group (Group C) and an REV-infected group (Group I). The chickens in Group I received intraperitoneal injections of REV with 10/0.1 mL TCID. Thymus was collected on day 1, 3, 7, 14, 21, 28, 35, and 49 for histopathology and assessed the status of oxidative stress.

Results: In chickens infected with REV, the levels of HO and MDA in the thymus increased, the levels of TAC, SOD, CAT, and GPx1 decreased, and there was a reduction in CAT and Gpx1 mRNA expression compared with the control group. The thymus index was also significantly reduced. Morphological analysis showed that REV infection caused an increase in the thymic reticular endothelial cells, inflammatory cell infiltration, mitochondrial swelling, and nuclear damage.

Conclusions: These results indicate that an increase in oxidative stress enhanced lipid peroxidation, markedly decreased antioxidant function, caused thymus atrophy, and immunosuppression in REV-infected chickens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12917-020-02708-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731740PMC
December 2020

Evaluation of Serum Exosomal lncRNAs as Diagnostic and Prognostic Biomarkers for Esophageal Squamous Cell Carcinoma.

Cancer Manag Res 2020 7;12:9753-9763. Epub 2020 Oct 7.

Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong Province, People's Republic of China.

Background: Exosomal long non-coding RNAs (lncRNAs) have been recognised as promising stable biomarkers in cancers. The aim of this study was to identify an exosomal lncRNA panel for diagnosis and prognosis of esophageal squamous cell carcinoma (ESCC).

Materials And Methods: Exosomes were isolated from serum by ExoQuick Solution. To validate the exosomes, exosomal markers and characterization of nanoparticle were performed. Quantitative real-time PCR was used to measure the levels of lncRNAs in exosomes from ESCC patients and healthy subjects. In the training set, exosomal lncRNA profiles from 404 samples were conducted and established new models by multivariate logistic regression. In the validation set, the diagnostic performance of the panel was further validated in 222 additional individuals with a receiver operating characteristic curve (ROC). Kaplan-Meier and multivariate Cox proportional hazards analysis were applied to assess the correlation between lncRNAs and survival rate of ESCC patients.

Results: A 4-lncRNA panel (UCA1, POU3F3, ESCCAL-1 and PEG10) in exosomes for ESCC diagnosis was developed by logistic regression model. The diagnostic accuracy of panel was evaluated with AUC value of 0.844 and 0.853 for training and validation stage, respectively. The corresponding AUCs for patients with TNM stage I-II and III were 0.820 and 0.935, significantly higher than squamous cell carcinoma antigen (<0.001), which were 0.652 and 0.642, respectively. Kaplan-Meier analysis indicated that patients with higher level of UCA1 and POU3F3 had lower survival rate (<0.001). Additionally, POU3F3 might be as an independent prognostic factor for ESCC patients (=0.004).

Conclusion: These findings suggested that serum exosomal 4-lncRNA panel has considerable value for ESCC diagnosis, and POU3F3 may serve as a novel and independent prognostic predictor in clinical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S250971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548224PMC
October 2020

Regulatory role and mechanism of the inhibition of the Mcl-1 pathway during apoptosis and polarization of H37Rv-infected macrophages.

Medicine (Baltimore) 2020 Oct;99(42):e22438

Department of Pathophysiology, the Key Laboratories for Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University.

Background: Myeloid cell leukemia-1 (Mcl-1) plays an important role in the clearance of Mycobacterium tuberculosis (MTB) infection. It has the effect of anti-apoptosis, protecting macrophages that have engulfed pathogens and preventing pathogen clearance. Meanwhile, the MAPK signaling pathway plays a significant role in regulating Mcl-1 expression during tuberculosis infection. In the case of latent infection and active infection, the apoptosis and polarization of macrophages have a great influence during MTB infection, so we discussed the effect of Mcl-1 on apoptosis and polarization. Then, further discussed its mechanism.

Methods: An infected RAW264.7 macrophage model was established to investigate the regulatory role and mechanism of the Mcl-1 pathway inhibition during apoptosis and polarization of H37Rv infection. First, Mcl-1 protein and mRNA was identified by western blotting and Real-Time Polymerase Chain Reaction (RT-PCR). RAW264.7 macrophage apoptosis was detected by flow cytometry. RT-PCR was utilized to detect Bax, Caspase-3, Cyt-c and Bcl-2 mRNA expression. Next, Then the expression levels of inflammation factors CD86, CD206, iNOS, Fizz1, IL-6, IL-10, TNF-α, and TGF-β was detected by ELISA. SEM was used to observe macrophages phenotype. Finally, Bax, Bcl-2 and Bcl-xl the expression was detected by western blotting. Confocal microscopy was used to analyze mitochondrial membrane potential using the JC-10 kit.

Results: In this study, we found that inhibiting the Mcl-1 expression signaling pathway led to infection by different virulence Mycobacterium tuberculosis, as well as changes in Mcl-1 protein and mRNA expression. Concomitantly macrophage apoptosis rate also changed, While, two phenotypic states of M1 and M2 appeared in the infected cells. We also found that the mitochondrial pathway was activated, the expression of its related genes Bax, casepase3, and Cyt-c, increased, whereas that of Bcl-2 decreased, and the mitochondrial membrane depolarization function was changed.

Conclusions: We found that Mcl-1 affected the apoptosis and polarization of macrophages infected by Mycobacterium tuberculosis, mainly M1 in the early stage and M2 in the later stage. In addition, mitochondria played a crucial role in this process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000022438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572003PMC
October 2020

Giant tuning of ferroelectricity in single crystals by thickness engineering.

Sci Adv 2020 Oct 14;6(42). Epub 2020 Oct 14.

School of Aerospace, Mechanical and Mechatronic Engineering, The University of Sydney, Sydney, NSW 2006, Australia.

Thickness effect and mechanical tuning behavior such as strain engineering in thin-film ferroelectrics have been extensively studied and widely used to tailor the ferroelectric properties. However, this is never the case in freestanding single crystals, and conclusions from thin films cannot be duplicated because of the differences in the nature and boundary conditions of the thin-film and freestanding single-crystal ferroelectrics. Here, using in situ biasing transmission electron microscopy, we studied the thickness-dependent domain switching behavior and predicted the trend of ferroelectricity in nanoscale materials induced by surface strain. We discovered that sample thickness plays a critical role in tailoring the domain switching behavior and ferroelectric properties of single-crystal ferroelectrics, arising from the huge surface strain and the resulting surface reconstruction. Our results provide important insights in tuning polarization/domain of single-crystal ferroelectric via sample thickness engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abc7156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556833PMC
October 2020

Differences in small noncoding RNAs profile between bull X and Y sperm.

PeerJ 2020 18;8:e9822. Epub 2020 Sep 18.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

The differences in small noncoding RNAs (sncRNAs), including miRNAs, piRNAs, and tRNA-derived fragments (tsRNAs), between X and Y sperm of mammals remain unclear. Here, we employed high-throughput sequencing to systematically compare the sncRNA profiles of X and Y sperm from bulls ( = 3), which may have a wider implication for the whole mammalian class. For the comparison of miRNA profiles, we found that the abundance of bta-miR-652 and bta-miR-378 were significantly higher in X sperm, while nine miRNAs, including bta-miR-204 and bta-miR-3432a, had greater abundance in Y sperm ( < 0.05). qPCR was then used to further validate their abundances. Subsequent functional analysis revealed that their targeted genes in sperm were significantly involved in nucleosome binding and nucleosomal DNA binding. In contrast, their targeted genes in mature oocyte were significantly enriched in 11 catabolic processes, indicating that these differentially abundant miRNAs may trigger a series of catabolic processes for the catabolization of different X and Y sperm components during fertilization. Furthermore, we found that X and Y sperm showed differences in piRNA clusters distributed in the genome as well as piRNA and tsRNA abundance, two tsRNAs (tRNA-Ser-AGA and tRNA-Ser-TGA) had lower abundance in X sperm than Y sperm ( < 0.05). Overall, our work describes the different sncRNA profiles of X and Y sperm in cattle and enhances our understanding of their potential roles in the regulation of sex differences in sperm and early embryonic development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.9822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505075PMC
September 2020

Constructing phase boundary in AgNbO antiferroelectrics: pathway simultaneously achieving high energy density and efficiency.

Nat Commun 2020 Sep 24;11(1):4824. Epub 2020 Sep 24.

Guangxi Key Laboratory of Processing for Non-ferrous Metallic and Featured Materials, School of Resources, Environment and Materials, Guangxi University, 530004, Nanning, China.

Dielectric capacitors with high energy storage density (W) and efficiency (η) are in great demand for high/pulsed power electronic systems, but the state-of-the-art lead-free dielectric materials are facing the challenge of increasing one parameter at the cost of the other. Herein, we report that high W of 6.3 J cm with η of 90% can be simultaneously achieved by constructing a room temperature M2-M3 phase boundary in (1-x)AgNbO-xAgTaO solid solution system. The designed material exhibits high energy storage stability over a wide temperature range of 20-150 °C and excellent cycling reliability up to 10 cycles. All these merits achieved in the studied solid solution are attributed to the unique relaxor antiferroelectric features relevant to the local structure heterogeneity and antiferroelectric ordering, being confirmed by scanning transmission electron microscopy and synchrotron X-ray diffraction. This work provides a good paradigm for developing new lead-free dielectrics for high-power energy storage applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-18665-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515927PMC
September 2020

Atomic-resolution electron microscopy of nanoscale local structure in lead-based relaxor ferroelectrics.

Nat Mater 2021 Jan 7;20(1):62-67. Epub 2020 Sep 7.

Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Relaxor ferroelectrics, which can exhibit exceptional electromechanical coupling, are some of the most important functional materials, with applications ranging from ultrasound imaging to actuators. Since their discovery, their complex nanoscale chemical and structural heterogeneity has made the origins of their electromechanical properties extremely difficult to understand. Here, we employ aberration-corrected scanning transmission electron microscopy to quantify various types of nanoscale heterogeneities and their connection to local polarization in the prototypical relaxor ferroelectric system Pb(MgNb)O-PbTiO. We identify three main contributions that each depend on Ti content: chemical order, oxygen octahedral tilt and oxygen octahedral distortion. These heterogeneities are found to be spatially correlated with low-angle polar domain walls, indicating their role in disrupting long-range polarization and leading to nanoscale domain formation and the relaxor response. We further locate nanoscale regions of monoclinic-like distortion that correlate directly with Ti content and electromechanical performance. Through this approach, the connections between chemical heterogeneity, structural heterogeneity and local polarization are revealed, validating models that are needed to develop the next generation of relaxor ferroelectrics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41563-020-0794-5DOI Listing
January 2021

Predictive value of S100A9 for lymph node metastasis in cervical cancer.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Jun;45(6):701-708

Department of Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.

Objectives: Lymph node metastasis affects the initial treatment strategy for cervical cancer and is hard to be diagnosed in clinical practice.This paper aims to explore the relationship between calcium-binding A9 (S100A9) and lymph node metastasis (LNM) in cervical cancer, and to determine the predictive value of S100A9 for LNM in cervical cancer.

Methods: We performed a retrospective cohort study and collected the pathological data, follow-up data, and paraffin tissue samples of 99 patients with cervical cancer who underwent modified extensive or extensive hysterectomy plus pelvic lymphadenectomy at the Department of Gynecology, Xiangya Hospital, Central South University from January 2013 to December 2018. Immunohistochemistry was used to detect the expression of S100A9 in cervical cancer tissues, and the correlation between S100A9 expression and LNM of cervical cancer, or clinicopathological characteristics were analyzed. The receiver operating characteristic (ROC) curve was used to establish a predictive model for LNM of cervical cancer, and Chi-square test of four-grid table was used to evaluate the diagnostic value of S100A9 for LNM in cervical cancer.

Results: The expression of S100A9 was significantly correlated with LNM. The S100A9 immunohistochemical semi-quantitative score of the LNM group was significantly higher than that in the non-lymph node metastasis group (<0.001). Moreover, the expression of S100A9 was significantly correlated with histological type, stromal invasion, lymphatic vessel invasion, or LNM (<0.05). The cut-off of the ROC curve for predicting LNM was 5, with the Youden index of 0.649 and the area under the ROC curve of 0.863. The disease-free survival and overall survival in the S100A9 positive group were significantly shorter than those in the negative group (<0.05). S100A9 alone had a sensitivity of 71.4%, a specificity of 91.5%, and an accuracy of 85.1% for diagnosing LNM. Imaging had a sensitivity of 32.1%, a specificity of 74.6%, and an accuracy of 60.9%. Combination of S100A9 with image examination in parallel test had a sensitivity of 85.7%, a specificity of 71.2%, and an accuracy of 75.9%, while combination of S100A9 and image examination in serial test had a sensitivity of 17.9%, a specificity of 98.3%, and an accuracy of 72.4%.

Conclusions: S100A9 may be associated with LNM in cervical cancer. S100A9 shows a promising perspective in predicting LNM in cervical cancer. Combination of S100A9 and image examination in serial test has a high specificity for LNM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11817/j.issn.1672-7347.2020.200059DOI Listing
June 2020

[Comparative study of three methods in the diagnosis of laryngopharyngeal reflux in children].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2020 Apr;34(4):343-345;350

Department of Otorhinolaryngology,Affiliated Hospital of Chengde Medical College,Chengde,067000,China.

To compare the consistency of RSI and RFS, pepsin detection and 24 h Dx-pH monitoring in the diagnosis of laryngopharyngeal reflux (LPR) in children. A retrospective analysis was made of 76 children with suspicious LPR. A total of 65 children with suspicious LPR were finally diagnosed. RSI and RFS scales, pepsin detection and Dx-pH monitoring were performed simultaneously. The diagnostic consistency of the three methods was tested, and the sensitivity and specificity were calculated. The sensitivity of pepsin detection, 24 h Dx-pH monitoring, RSI and RFS in the diagnosis of LPR in 76 suspected LPR patients were 95.31%, 66.15% and 84.62%, and the specificity were 100%, 81.82% and 72.73%. The pepsin detection was well consistent with the scale(Kappa>0.75), pepsin detection and 24 h Dx-pH monitoring were in medium consistency(Kappa=0.467), and 24 h Dx-pH monitoring and scale were in medium consistency(Kappa=0.446). Pepsin detection can be used as a reliable index for the diagnosis of LPR in children. It is objective, economical, non-invasive, comfortable and easy to spread.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13201/j.issn.2096-7993.2020.04.014DOI Listing
April 2020

Unveiling the ferrielectric nature of PbZrO-based antiferroelectric materials.

Nat Commun 2020 Jul 30;11(1):3809. Epub 2020 Jul 30.

State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 200050, Shanghai, China.

Benefitting from the reversible phase transition between antiferroelectric and ferroelectric states, antiferroelectric materials have recently received widespread attentions for energy storage applications. Antiferroelectric configuration with specific antiparallel dipoles has been used to establish antiferroelectric theories and understand its characteristic behaviors. Here, we report that the so-called antiferroelectric (Pb,La)(Zr,Sn,Ti)O system is actually ferrielectric in nature. We demonstrate different ferrielectric configurations, which consists of ferroelectric ordering segments with either magnitude or angle modulation of dipoles. The ferrielectric configurations are mainly contributed from the coupling between A-cations and O-anions, and their displacement behavior is dependent largely on the chemical doping. Of particular significance is that the width and net polarization of ferroelectric ordering segments can be tailored by composition, which is linearly related to the key electrical characteristics, including switching field, remanent polarization and dielectric constant. These findings provide opportunities for comprehending structure-property correlation, developing antiferroelectric/ferrielectric theories and designing novel ferroic materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-17664-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392892PMC
July 2020

Common and distinct functional stability abnormalities across three major psychiatric disorders.

Neuroimage Clin 2020 17;27:102352. Epub 2020 Jul 17.

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. Electronic address:

Delineating the neuropathological characteristics across psychiatric disorders is critical for understanding their pathophysiology. The purpose of this study was to investigate common and distinct brain functional abnormalities across psychiatric disorders by using functional stability, a recently developed dynamic functional connectivity approach. Resting-state functional magnetic resonance imaging data were collected from a transdisease sample of healthy controls (n = 115) and individuals with schizophrenia (SZ) (n = 47), bipolar disorder (BD) (n = 44), and attention deficit/hyperactivity disorder (ADHD) (n = 40). Functional stability of each voxel was calculated by measuring the concordance of dynamic functional connectivity over time. Differences in functional stability among the four groups were assessed voxel-wisely. Compared to healthy controls, individuals with SZ demonstrated a distributed pattern of higher functional stability in the bilateral inferior temporal gyrus yet lower stability in the bilateral calcarine sulcus and left insula; individuals with BD only manifested local higher stability in the left inferior temporal gyrus; no differences were found between ADHD and healthy individuals. Notably, individuals with SZ and BD had common higher functional stability in the left inferior temporal gyrus, whereas higher functional stability in the right inferior temporal gyrus and lower stability in the bilateral calcarine sulcus and left insula were unique abnormalities in individuals with SZ. Additionally, direct comparisons between disorders revealed that individuals with SZ showed lower functional stability in the right calcarine sulcus compared to those with BD and higher stability in the left inferior temporal gyrus compared to those with ADHD. However, no significant associations between functional stability and clinical symptoms were observed. Our findings suggest that the functional stability approach has the potential to be extended to the domain of psychiatry and encourage further investigations of shared and unique neuropathology of psychiatric disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nicl.2020.102352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393318PMC
July 2020

Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.

Biosci Biotechnol Biochem 2020 Nov 11;84(11):2228-2240. Epub 2020 Jul 11.

Chongqing Key Laboratory of Infectious Diseases and Parasitic Diseases, Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University , Chongqing, China.

Ginsenoside Rg1 (G-Rg1) is a bioactive phytochemical that has been found to be beneficial for the treatment of several diseases including nonalcoholic fatty liver disease (NAFLD). But there is a lack of literature reporting the effect of G-Rg1 on lipid metabolism balance in NAFLD. We investigated the effect and mechanism of G-Rg1 on lipid metabolism . We found that G-Rg1 decreased the levels of TG, TC, and MDA, and increased activity of SOD. Results of RT-PCR and western blotting showed that supplementation with G-Rg1 downregulated the expression of PPAR γ, FABP1, FATP2/5, CD36, SREBP1 c, and FASN, while the expression of PPAR ɑ, CPT1, ACOX1, MTTP, and ApoB100 was upregulated, after induction by a free fatty acid. Taken together, we conclude that G-Rg1 inhibits lipid synthesis and lipid uptake, and enhances lipid oxidation and lipid export to reduce hepatic steatosis of HepG2 cells by regulating PPAR ɑ and PPAR γ expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09168451.2020.1793293DOI Listing
November 2020