Publications by authors named "Shufeng Fan"

13 Publications

  • Page 1 of 1

A multi-class scoring system based on CT features for preoperative prediction in gastric gastrointestinal stromal tumors.

Am J Cancer Res 2020 1;10(11):3867-3881. Epub 2020 Nov 1.

Department of Radiology, Second Affiliated Hospital, School of Medicine, Zhejiang University 88 Jiefang Road, Hangzhou 310009, Zhejiang Province, China.

Our study aimed to establish and validate a multi-class scoring system for preoperative gastric gastrointestinal stromal tumors (GISTs) risk stratifications based on CT features. 150 gastric GIST patients who underwent contrast-enhanced CT examination and surgical resection from hospital 1 were retrospectively analyzed as the training cohort, and 61 patients from hospitals 2 and 3 were included as the validation cohort. A model was established by logistic regression analysis and weighted to be a scoring model. A calibration test, area under the receiver operating characteristic (ROC) curve (AUC), and cutoff points were determined for the score model. The model was also divided into three score ranges for convenient clinical evaluation. Five CT features were included in the score model, including tumor size (4 points), ill-defined margin (6 points), intratumoral enlarged vessels (5 points), heterogeneous enhancement pattern (4 points), and exophytic or mixed growth pattern (2 points). Then, based on the calibration results, performance was merely assessed as very low and high* risk. The AUCs of the score model for very low risk and high* risk were 0.973 and 0.977, and the cutoff points were 3 points (97.30%, 93.81%) and 7 points (92.19%, 94.19%), respectively. In the validation cohort, the AUCs were 0.912 and 0.972, and the cutoff values were 3 points (92.31%, 85.42%) and 5 points (100%, 87.88%), respectively. The model was stratified into 3 ranges: 0-3 points for very low risk, 4-8 points for low risk, and 9-21 points for high* risk. A concise and practical score system for gastric GISTs risk stratification was proposed.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716157PMC
November 2020

Multimodality magnetic resonance imaging for the diagnosis of high-flow priapism following a straddle injury: A case report.

Medicine (Baltimore) 2020 Oct;99(41):e22618

Department of Radiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hang Zhou, Zhejiang, China.

Rationale: Priapism is a common urologic emergency, but high-flow penile priapism (HFP) caused by trauma is very rare. Therefore, HFP diagnosis and treatment are still not standardized.

Patient Concerns: A 29-year-old man was admitted to the urology department of our hospital on August 01, 2019, due to "persistent penile erection caused by a straddle injury."

Diagnosis: On July 17, 2019, the patient underwent Doppler ultrasonography, which indicated swollen corpus cavernosum.

Interventions: The patient took over-the-counter anti-inflammatory drugs but the erectile state of the penis remained unchanged. A second perineal injury resulted in hospital admission. Multimodality magnetic resonance imaging (MRI) scan showed nodular abnormal signals at the right corpus cavernosum root. Subsequently, selective arterial interventional angiography confirmed the MRI findings. Spring coils were then inserted for embolization, and the pseudoaneurysm, fistula, and priapism disappeared.

Outcomes: Two months after surgery, sexual stimuli could normally cause penile erection, with normal hardness. The patient's sexual life returned to normal 3 months after surgery.

Conclusion: Multimodality MRI is very effective in detecting high blood flow priapism. Its application would improve the clinical management of this ailment.
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http://dx.doi.org/10.1097/MD.0000000000022618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544374PMC
October 2020

Submerged Vegetation and Water Quality Degeneration From Serious Flooding in Liangzi Lake, China.

Front Plant Sci 2019 25;10:1504. Epub 2019 Nov 25.

The National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Science, Wuhan University, Wuhan, China.

In shallow lake ecosystems, flooding is a key disturbance factor of aquatic vegetation. Aquatic plants, especially submerged plants, play key roles in water ecosystems. Liangzi Lake experienced severe flooding in July 2010, and the elevated water levels lasted for 3 months. In this study, 10 transects with 120 monitoring points were set up for monthly monitoring during the 3-year period, encompassing the period before and after the flooding (2009-2011). The numbers, biomass, and diversity of the submerged plants, as well as the physical and chemical characteristics of the lake water, were surveyed. There were 12 species belonging to 7 families and 7 genera in Liangzi Lake. Eleven of the submerged plant species were found in 2009, but, after the flood, that number decreased to five in 2011. The total biomass differed significantly over the three years ( < 0.05), with the largest biomass in 2009 and smallest in 2011. In 2009 and 2010, was the dominant species, but its dominant position weakened in 2011. After the flood, water transparency decreased, and the water depth, turbidity, total nitrogen, and total phosphorus increased. A redundancy analysis between the submerged plants and environmental factors found that the water transparency, turbidity, and water depth were the key environmental factors affecting the plants. These results suggest that the long-lasting severe flooding of Liangzi Lake in 2010 led to the degradation of both the submerged plant community and water quality.
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http://dx.doi.org/10.3389/fpls.2019.01504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886514PMC
November 2019

Effects of Eutrophication and Different Water Levels on Overwintering of at the Northern Margin of Its Distribution in China.

Front Plant Sci 2019 4;10:1261. Epub 2019 Oct 4.

The National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Ecology, Wuhan University, Wuhan, China.

When exotic species are introduced into new areas, establishment is a vital step in their invasion process. Therefore, overwintering strategies determine whether an exotic species from low latitudes can successfully invade middle- and high-latitude areas. In this study, we investigated the effects of nutrient and water drawdown on overwintering in an exotic aquatic plant from the tropical zone, , at the northern margin of its distribution in China. The population density, size of individuals, and the size and nitrogen concentration of overwintering organs (stem base) of that grew in high-nutrition water were greater than those that grew in low-nutrient water before winter. The overwinter survival rate of was significantly affected by the water level and nutrient. The thick and dense floating mat of can increase the temperature of water bodies; therefore, the overwinter survival rate of was higher in constant-water-level and high-nutrient treatment. In contrast, due to the loss of heat preservation provided by the floating mats and the low nitrogen concentration in the stem base, all individuals of died in constant-water-level and low-nutrient treatment. In the water-drawdown treatments, the stem base of was directly exposed to low-temperature air; therefore, the overwinter survival rate of was lower. Our results reveal that eutrophication can not only improve the competitiveness of but can also improve the survival rate of overwintering plants in temperate regions. Our study also suggests that removing nutrients from the water and regulating the water level can limit the invasion of in temperate and subtropical regions.
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http://dx.doi.org/10.3389/fpls.2019.01261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788430PMC
October 2019

Coverage of Native Plants Is Key Factor Influencing the Invasibility of Freshwater Ecosystems by Exotic Plants in China.

Front Plant Sci 2018 28;9:250. Epub 2018 Feb 28.

The National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Science, Wuhan University, Wuhan, China.

Understanding the biotic and abiotic factors that influence the susceptibility of a community to invasion is beneficial for the prediction and management of invasive species and the conservation of native biodiversity. However, the relationships between factors and invasibility of a community have not been fully confirmed, and the factors most associated with the susceptibility of a community to invasion have rarely been identified. In this study, we investigated the species richness patterns in aquatic exotic and native plants and the relationships of exotic species richness with habitat and water environment factors in 262 aquatic plant communities in China. A total of 11 exotic plant species were recorded in our field survey, and we found neither a negative nor a positive relationship between aquatic exotic and native plant species richness. The aquatic exotic plant species richness is negatively correlated with the relative coverage and biomass of native plants but positively correlated with the total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) concentrations in the water. The native plant species richness, native species' relative coverage, and native species' biomass were positively related to each other, whereas the TP, TN, and COD were also positively related to each other. The native plant species richness, native species' relative coverage, and native species biomass were each negatively correlated with the TP, TN, and COD. In addition, biotic rather than abiotic predictors accounted for most of the variation in exotic plant richness. Our results suggest that improving the vegetation coverage and the biodiversity of native plants is the most effective approach for preventing alien plant invasions and minimizing their impacts on freshwater ecosystems.
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http://dx.doi.org/10.3389/fpls.2018.00250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863531PMC
February 2018

Invasive plant Alternanthera philoxeroides suffers more severe herbivory pressure than native competitors in recipient communities.

Sci Rep 2016 11 9;6:36542. Epub 2016 Nov 9.

Department of Ecology, Wuhan University, Wuhan, 430072, P.R. China.

Host-enemy interactions are vital mechanisms that explain the success or failure of invasive plants in new ranges. We surveyed the defoliation of invasive Alternanthera philoxeroides and co-occurring native plants on two islands during different seasons over three consecutive years and measured the leaf nitrogen content and the C/N ratio of each plant species. To evaluate the effects of herbivory on A. philoxeroides, an herbivore exclosure experiment was conducted. We found that the mean defoliation of A. philoxeroides was higher than that of native plants, regardless of whether the dominant species was A. philoxeroides or native plants. A. philoxeroides defoliation increased significantly as the months progressed, whereas the defoliation of the total population of native plants was constant. The leaf nitrogen content was positively correlated with defoliation, and it was highest in A. philoxeroides. Additionally, A. philoxeroides in the herbivore exclusion treatment showed an increase in shoot biomass and total shoot length. Our study revealed that native generalist herbivores prefer the invasive plant to the natives because of the higher leaf nitrogen content. These results support the biotic resistance hypothesis, suggesting that native herbivore species can limit the population spread of invasive plants.
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http://dx.doi.org/10.1038/srep36542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101519PMC
November 2016

[Value of magnetic resonance imaging for early diagnosis of prostatic carcinoma in central zone].

Zhonghua Yi Xue Za Zhi 2014 Apr;94(14):1051-4

Department of Urinary Surgery, Taizhou Hospital, Linhai 317000, China. Email:

Objective: To explore the magnetic resonance imaging (MRI) manifestations of early prostatic carcinoma (PCa) in central zone (CZ) .

Methods: A total of 284 cases of PCa were scanned by MRI while 24 cases of carcinoma in 28 PCa focus were in central zone. Their T1WI, T2WI, DWI and ADC value characteristics were analyzed respectively and the completeness of annular low signal between central and peripheral zones were observed. At the same time, 45 cases of PCa were located in peripheral zone (PZ) . Twenty cases of benign prostatic hyperplasia (BPH) were comparatively analyzed. All cases received a histological diagnosis.

Results: Most cases of early PCa in CZ demonstrated ill-defined margin, amorphous shape and equal signal intensity on T1WI, low signal intensity on T2WI and high signal intensity on DWI. The mean ADC value for PCa focus was (0.867 ± 0.074)×10(-3) mm(2)/s and PCa foci were enhanced slightly. In these cases, most annular low signals between CZ and PZ were destroyed. The difference of ADC values between PCa in CZ and PZ groups ((0.914 ± 0.066) ×10(-3) mm(2)/s ) had no statistic significance (P > 0.05) , but the ADC values between PCa and BPH in CZ groups ((1.517 ± 0.315) ×10(-3) mm(2)/s ) had statistically significant difference (P < 0.05).

Conclusion: Some reliable diagnostic characteristics on MRI may be used for an early diagnosis of PCa in CZ.
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April 2014

An invasive clonal plant benefits from clonal integration more than a co-occurring native plant in nutrient-patchy and competitive environments.

PLoS One 2014 9;9(5):e97246. Epub 2014 May 9.

The National Field Station of Lake Ecosystem of Liangzi Lake, College of Life Science, Wuhan University, Wuhan, P.R. China.

Many notorious invasive plants are clonal, however, little is known about the different roles of clonal integration effects between invasive and native plants. Here, we hypothesize that clonal integration affect growth, photosynthetic performance, biomass allocation and thus competitive ability of invasive and native clonal plants, and invasive clonal plants benefit from clonal integration more than co-occurring native plants in heterogeneous habitats. To test these hypotheses, two stoloniferous clonal plants, Alternanthera philoxeroides (invasive), Jussiaea repens (native) were studied in China. The apical parts of both species were grown either with or without neighboring vegetation and the basal parts without competitors were in nutrient- rich or -poor habitats, with stolon connections were either severed or kept intact. Competition significantly reduced growth and photosynthetic performance of the apical ramets in both species, but not the biomass of neighboring vegetation. Without competition, clonal integration greatly improved the growth and photosynthetic performance of both species, especially when the basal parts were in nutrient-rich habitats. When grown with neighboring vegetation, growth of J. repens and photosynthetic performance of both species were significantly enhanced by clonal integration with the basal parts in both nutrient-rich and -poor habitats, while growth and relative neighbor effect (RNE) of A. philoxeroides were greatly improved by clonal integration only when the basal parts were in nutrient-rich habitats. Moreover, clonal integration increased A. philoxeroides's biomass allocation to roots without competition, but decreased it with competition, especially when the basal ramets were in nutrient-rich sections. Effects of clonal integration on biomass allocation of J. repens was similar to that of A. philoxeroides but with less significance. These results supported our hypothesis that invasive clonal plants A. philoxeroides benefits from clonal integration more than co-occurring native J. repens, suggesting that the invasiveness of A. philoxeroides may be closely related to clonal integration in heterogeneous environments.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097246PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016286PMC
June 2015

The invasive plant Alternanthera philoxeroides was suppressed more intensively than its native congener by a native generalist: implications for the biotic resistance hypothesis.

PLoS One 2013 26;8(12):e83619. Epub 2013 Dec 26.

The National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Science, Wuhan University, Wuhan, P.R. China.

Prior studies on preferences of native herbivores for native or exotic plants have tested both the enemy release hypothesis and the biotic resistance hypothesis and have reported inconsistent results. The different levels of resistance of native and exotic plants to native herbivores could resolve this controversy, but little attention has been paid to this issue. In this study, we investigated population performance, photosynthesis, leaf nitrogen concentration, and the constitutive and induced resistances of the successful invasive plant, Alternanthera philoxeroides, and its native congener, Alternanthera sessilis, in the presence of three population densities of the grasshopper, Atractomorpha sinensis. When the grasshopper was absent, leaf biomass, total biomass, photosynthesis, and leaf nitrogen concentration of A. philoxeroides were higher than those of A. sessilis. However, the morphological and physiological performances of A. philoxeroides were all decreased more intensively than A. sessilis after herbivory by grasshoppers. Especially as the concentrations of constitutive lignin and cellulose in leaf of A. philoxeroides were higher than A. sessilis, A. philoxeroides exhibited increased leaf lignin concentration to reduce its palatability only at severe herbivore load, whereas, leaf lignin, cellulose, and polyphenolic concentrations of A. sessilis all increased with increasing herbivory pressure, and cellulose and polyphenolic concentrations were higher in A. sessilis than in A. philoxeroides after herbivory. Our study indicated that the capability of the invasive plant to respond to native insect damage was lower than the native plant, and the invasive plant was suppressed more intensively than its native congener by the native insect. Our results support the biotic resistance hypothesis and suggest that native herbivores can constrain the abundance and reduce the adverse effects of invasive species.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083619PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873342PMC
September 2014

Screening and identification of six serum microRNAs as novel potential combination biomarkers for pulmonary tuberculosis diagnosis.

PLoS One 2013 5;8(12):e81076. Epub 2013 Dec 5.

Institute of Cell Biology, Zhejiang University, Hangzhou, China.

Background: It is very difficult to prevent pulmonary tuberculosis (TB) due to the lack of specific and diagnostic markers, which could lead to a high incidence of pulmonary TB. We screened the differentially expressed serum microRNAs (miRNAs) as potential biomarkers for the diagnosis of pulmonary TB.

Methods: In this study, serum miRNAs were screened using the Solexa sequencing method as the potential biomarkers for the diagnosis of pulmonary TB. The stem-loop quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assay was used to verify differentially expressed serum miRNAs. The receiver operating characteristic (ROC) curve and logistic regression model were used to analyze the sensitivity and specificity of the single miRNA and a combination of miRNAs for diagnosis, respectively. Using the predicted target genes, we constructed the regulatory networks of miRNAs and genes that were related to pulmonary TB.

Results: The Solexa sequencing data showed that 91 serum miRNAs were differentially expressed in pulmonary TB patients, compared to healthy controls. Following qRT-PCR confirmation, six serum miRNAs (hsa-miR-378, hsa-miR-483-5p, hsa-miR-22, hsa-miR-29c, hsa-miR-101 and hsa-miR-320b) showed significant difference among pulmonary TB patients, healthy controls (P<0.001) and differential diagnosis groups (including patients with pneumonia, lung cancer and chronic obstructive pulmonary disease) (P<0.05). The logistic regression analysis of a combination of six serum miRNAs revealed that the sensitivity and the specificity of TB diagnosis were 95.0% and 91.8% respectively. The miRNAs-gene regulatory networks revealed that several miRNAs may regulate some target genes involved in immune pathways and participate in the pathogenesis of pulmonary TB.

Conclusion: Our study suggests that a combination of six serum miRNAs have great potential to serve as non-invasive biomarkers of pulmonary TB.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081076PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857778PMC
September 2014

In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.

PLoS One 2012 17;7(1):e30262. Epub 2012 Jan 17.

Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, China.

Objectives: Thrombus and secondary thrombosis plays a key role in stroke. Recent molecular imaging provides in vivo imaging of activated factor XIII (FXIIIa), an important mediator of thrombosis or fibrinolytic resistance. The present study was to investigate the fibrin deposition in a thromboembolic stroke mice model by FXIIIa-targeted near-infrared fluorescence (NIRF) imaging.

Materials And Methods: The experimental protocol was approved by our institutional animal use committee. Seventy-six C57B/6J mice were subjected to thromboembolic middle cerebral artery occlusion or sham operation. Mice were either intravenously injected with the FXIIIa-targeted probe or control probe. In vivo and ex vivo NIRF imaging were performed thereafter. Probe distribution was assessed with fluorescence microscopy by spectral imaging and quantification system. MR scans were performed to measure lesion volumes in vivo, which were correlated with histology after animal euthanasia.

Results: In vivo significant higher fluorescence intensity over the ischemia-affected hemisphere, compared to the contralateral side, was detected in mice that received FXIIIa-targeted probe, but not in the controlled mice. Significantly NIRF signals showed time-dependent processes from 8 to 96 hours after injection of FXIIIa-targeted probes. Ex vivo NIRF image showed an intense fluorescence within the ischemic territory only in mice injected with FXIIIa-targeted probe. The fluorescence microscopy demonstrated distribution of FXIIIa-targeted probe in the ischemic region and nearby micro-vessels, and FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images. There was a significant correlation between total targeted signal from in vivo or ex vivo NIRF images and lesion volume.

Conclusion: Non-invasive detection of fibrin deposition in ischemic mouse brain using NIRF imaging is feasible and this technique may provide an in vivo experimental tool in studying the role of fibrin in stroke.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030262PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260250PMC
June 2012

[Analysis of result and influence factors of operative treatment of acetabular fractures].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2011 Jan;25(1):21-5

Department of Pelvis and Acetabulum of Shenyang Orthopaedic Hospital, Shenyang Liaoning, 110044, P.R. China.

Objective: To evaluate the results of operative treatment of acetabular fractures and to investigate its influence factors.

Methods: The clinical data were analyzed retrospectively from 82 patients with acetabular fractures treated between September 2004 and June 2009. Of 82 patients, 65 were male and 17 were female, aged 26-72 years (mean, 38 years). Fractures were caused by traffic accident in 62 cases, by crush in 13 cases, and by falling from height in 7 cases. The time from injury to admission was 30 minutes to 12 days (median, 7.6 hours) in 70 cases, 12 cases were transferred because poor result after 34-67 days of conservative treatment. According to Judet classification, there were 24 cases of posterior wall fracture, 3 cases of posterior column fracture, 1 case of anterior wall fracture, 2 cases of anterior column fracture, 6 cases of transverse fracture, 16 cases of transverse and posterior wall fracture, 4 cases of posterior column and posterior wall fracture, 5 cases of T-type fracture, 3 cases of anterior and posterior hemitransverse fracture, and 18 cases of complete both-column fracture; 24 cases combined with dislocation of the hip. During operation, Kocher-Langenbeck approach was used in 49 cases, anterior ilioinguinal approach in 19 cases, and the combination of anterior and posterior approaches in 14 cases. Reconstructive plate (74 cases) and hollow lag screw (8 cases) internal fixation were used. The function of the hip was evaluated according to the modified Merled'Aubigne-Postel hip score system postoperatively. According to fracture type, age, lower extremity fracture before operation, quality of reduction, timing of surgery, hip dislocation and time of reduction, operative approach, deep vein thrombosis (DVT), and heterotopic ossification (HO), the patients were divided into the groups and the results were compared.

Results: Accordance with the Matta X-ray evaluation criteria, anatomic reduction was achieved in 21 cases, good reduction in 37 cases, fair reduction in 16 cases, and poor reduction in 8 cases, and the excellent and good rate was 71%. All the cases were followed up 12-52 months (mean, 34 months). Iatrogenic sciatic nerve injury occurred in 8 cases, infection in 3 cases, HO in 16 cases, DVT in 3 cases, hip posttraumatic arthritis in 12 cases, and avascular necrosis of the femoral head in 9 cases. X-ray examination showed that 80 cases achieved fracture union at 10-24 weeks after operation (mean, 14 weeks) and 2 cases had fracture delayed union at 10 months and 12 months after operation. According to the modified Merled'Aubigne-Postel hip score system, the function of the hip was rated as excellent in 26 cases, good in 32, fair in 20, and poor in 4 at 6 months after operation; the excellent and good rate was 71%. The affecting factors of clinical results of acetabular fractures were fracture type, age, lower extremity fracture before operation, quality of reduction, timing of surgery, hip dislocation and time of reduction (P < 0.05). However the operative approach, DVT, and HO were not affecting factor of the acetabular fractures (P > 0.05).

Conclusion: Operative treatment of acetabular fractures has a satisfying therapeutic effect. Fracture type, age, lower extremity fracture before operation, quality of reduction, timing of surgery, hip dislocation, and time of reduction are risk factors affecting postoperative results.
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January 2011