Publications by authors named "Shuangling Li"

17 Publications

  • Page 1 of 1

Deglycosylated Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis via the TGF-β1 Signaling Pathway.

Molecules 2021 May 10;26(9). Epub 2021 May 10.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300000, China.

Idiopathic pulmonary fibrosis (IPF) is a progressive, life-threatening lung disease characterized by the proliferation of myofibroblasts and deposition of extracellular matrix that results in irreversible distortion of the lung structure and the formation of focal fibrosis. The molecular mechanism of IPF is not fully understood, and there is no satisfactory treatment. However, most studies suggest that abnormal activation of transforming growth factor-β1 (TGF-β1) can promote fibroblast activation and epithelial to mesenchymal transition (EMT) to induce pulmonary fibrosis. Deglycosylated azithromycin (Deg-AZM) is a compound we previously obtained by removing glycosyls from azithromycin; it was demonstrated to exert little or no antibacterial effects. Here, we discovered a new function of Deg-AZM in pulmonary fibrosis. experiments showed that Deg-AZM could significantly reduce bleomycin-induced pulmonary fibrosis and restore respiratory function. Further study revealed the anti-inflammatory and antioxidant effects of Deg-AZM . experiments showed that Deg-AZM inhibited TGF-β1 signaling, weakened the activation and differentiation of lung fibroblasts, and inhibited TGF-β1-induced EMT in alveolar epithelial cells. In conclusion, our findings show that Deg-AZM exerts antifibrotic effects by inhibiting TGF-β1-induced myofibroblast activation and EMT.
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http://dx.doi.org/10.3390/molecules26092820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126120PMC
May 2021

Novel approach of rigid bronchoscopy concurrent with cesarean section and bronchial arterial embolism for patients with massive hemoptysis during pregnancy: case reports and literature review.

Ann Transl Med 2021 Apr;9(7):582

Department of Critical Care Medicine, Peking University First Hospital, Beijing, China.

Massive hemoptysis can be life-threatening and is frequently encountered in clinical practice, but rare during pregnancy. There have been limited case reports of massive hemoptysis in pregnancy in patients with conditions such as Takayasu's arteritis, bronchiectasis, bronchial carcinoid tumor, and tuberculosis. The most important management is early control of the hemorrhage and airway protection. We report on 2 patients at 33 and 27 gestational weeks who were admitted to the emergency department because of massive hemoptysis. Therapeutic rigid bronchoscopy with the application of high-frequency jet ventilation was performed under general anesthesia during cesarean section to control potential bleeding and stabilize the airway; this was then followed by bronchial artery embolization (BAE) postsurgically. The lives of both mothers and infants were saved. At the 16- and 11-month follow-ups, the patients showed no symptoms. To our knowledge, this is the first report on the application of therapeutic rigid bronchoscopy concurrent with cesarean section in order to protect the airway and reduce the side effects of the subsequent treatment for both mother and fetus in hemoptysis cases. By reporting these cases and conducting a literature review, we present a novel treatment method for massive hemoptysis in pregnant patients that may improve patients' outcomes.
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http://dx.doi.org/10.21037/atm-20-2502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105789PMC
April 2021

The complete chloroplast genome of (With.) P. Beauv.

Mitochondrial DNA B Resour 2021 Mar 11;6(3):837-839. Epub 2021 Mar 11.

College of Life Sciences, Hebei Normal University, Shijiazhuang, PR China.

is the type genus of the family Mniaceae, the species' richest family of order Bryales (Bryophyte). is widely distributed worldwide. The complete chloroplast genome of was sequenced by high-throughput sequencing technology. The chloroplast genome is 124,935 bp in length with a quadripartite structure. The two inverted repeat regions are 9,910 bp long and separated by a large single-copy region of 86,572 bp and a small single-copy region of 18,543 bp. Based on the chloroplast genome data of 11 bryophytes downloaded from GenBank and one acquired in this study, phylogenetic trees were constructed.
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http://dx.doi.org/10.1080/23802359.2021.1884025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954419PMC
March 2021

EZH2 Dynamically Associates With Non-coding RNAs in Mouse Hearts After Acute Angiotensin II Treatment.

Front Cardiovasc Med 2021 25;8:585691. Epub 2021 Feb 25.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Enhancer of zeste 2 (EZH2) governs gene reprogramming during cardiac hypertrophy through epigenetic remodeling, a process regulated by numerous non-coding RNAs (ncRNAs). However, the dynamic interaction between EZH2 and ncRNAs upon hypertrophic stimulation remains elusive. Here we performed an unbiased profiling for EZH2-associated ncRNAs in mouse hearts treated with Angiotensin II (AngII) at different time points (0, 4, and 24 h). The interactions between EZH2 and long ncRNAs (lncRNAs), Chaer, Mirt1, Hotair, and H19, were validated by PCR. RIP-seq analysis identified a total of 126 ncRNAs to be significantly associated with EZH2. These ncRNAs covers all five categories including intergenic, antisense, intron-related, promoter-related and both antisense and promoter-related. According to their changing patterns after AngII treatment, these ncRNAs were clustered into four groups, constantly enhanced, transiently enhanced, constantly suppressed and transiently suppressed. Structural prediction showed that EZH2 bound to hairpin motifs in ncRNAs including snoRNAs. Interaction strength prediction and RNA pull-down assay confirmed the direct interaction between EZH2 and Snora33. Interestingly, two antisense lncRNAs of Malat1, Gm20417, and Gm37376, displayed different binding patterns from their host gene after AngII treatment, suggesting a crucial role of this genomic locus in modulating EZH2 behavior. Our findings reveal the profile of EZH2-associated ncRNAs upon hypertrophic stimulation, and imply a dynamic regulation of EZH2 function in cardiac hypertrophy.
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http://dx.doi.org/10.3389/fcvm.2021.585691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959742PMC
February 2021

Glutamine on critical-ill patients: a systematic review and meta-analysis.

Ann Palliat Med 2021 Feb 19;10(2):1503-1520. Epub 2020 Nov 19.

Department of Critical Care Medicine, First Hospital of Shanxi Medical University, Taiyuan, China.

Background: To conduct a meta-analysis of the effect of glutamine supplements on prognosis in adult critical-ill patients.

Methods: We searched the Web of Science, Cochrane library, PubMed, the Wanfang Database, and the China National Knowledge Infrastructure (CNKI)/CBMdisc database. The primary outcome was hospital mortality, or if not reported, 28-day/6-month/intensive care unit (ICU) mortality. The secondary outcomes were duration of mechanical ventilation (MV), length of stay (LOS) in the ICU, LOS in the hospital, and nosocomial infections.

Results: In 599 related articles, 47 randomized controlled trials, including 6,198 patients, met all the inclusion criteria. Hospital mortality was not significantly different between the glutamine group and the control group. Length of MV was significantly higher in the control group than that of the glutamine group. In a subgroup analysis of severely burned patients, hospital mortality had the same trend. In other subgroups, there was no significant difference between the two groups.

Conclusions: We suggest that supplemental glutamine need not be routinely added to the diet of criticalill patients to reduce hospital mortality, with the exception of the diet of severely burned patients.
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http://dx.doi.org/10.21037/apm-20-702DOI Listing
February 2021

Human amniotic epithelial cells ameliorate kidney damage in ischemia-reperfusion mouse model of acute kidney injury.

Stem Cell Res Ther 2020 09 23;11(1):410. Epub 2020 Sep 23.

Renal Division, Peking University First Hospital, Peking University Institute of Nephrology, Beijing, 100034, People's Republic of China.

Background: Acute kidney injury (AKI) is a common clinical disease with complex pathophysiology and limited therapeutic choices. This prompts the need for novel therapy targeting multiple aspects of this disease. Human amnion epithelial cell (hAEC) is an ideal stem cell source. Increasing evidence suggests that exosomes may act as critical cell-cell communicators. Accordingly, we assessed the therapeutic potential of hAECs and their derived exosomes (hAECs-EXO) in ischemia reperfusion mouse model of AKI and explored the underlying mechanisms.

Methods: The hAECs were primary cultured, and hAECs-EXO were isolated and characterized. An ischemic-reperfusion injury-induced AKI (IRI-AKI) mouse model was established to mimic clinical ischemic kidney injury with different disease severity. Mouse blood creatinine level was used to assess renal function, and kidney specimens were processed to detect cell proliferation, apoptosis, and capillary density. Macrophage infiltration was analyzed by flow cytometry. hAEC-derived exosomes (hAECs-EXO) were used to treat hypoxia-reoxygenation (H/R) injured HK-2 cells and mouse bone marrow-derived macrophages to evaluate their protective effect in vitro. Furthermore, hAECs-EXO were subjected to liquid chromatography-tandem mass spectrometry for proteomic profiling.

Results: We found that systematically administered hAECs could improve mortality and renal function in IRI-AKI mice, decrease the number of apoptotic cells, prevent peritubular capillary loss, and modulate kidney local immune response. However, hAECs showed very low kidney tissue integration. Exosomes isolated from hAECs recapitulated the renal protective effects of their source cells. In vitro, hAECs-EXO protected HK-2 cells from H/R injury-induced apoptosis and promoted bone marrow-derived macrophage polarization toward M2 phenotype. Proteomic analysis on hAECs-EXO revealed proteins involved in extracellular matrix organization, growth factor signaling pathways, cytokine production, and immunomodulation. These findings demonstrated that paracrine of exosomes might be the key mechanism of hAECs in alleviating renal ischemia reperfusion injury.

Conclusions: We reported hAECs could improve survival and ameliorate renal injury in mice with IRI-AKI. The anti-apoptotic, pro-angiogenetic, and immunomodulatory capabilities of hAECs are at least partially, through paracrine pathways. hAECs-EXO might be a promising clinical therapeutic tool, overcoming the weaknesses and risks associated with the use of native stem cells, for patients with AKI.
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http://dx.doi.org/10.1186/s13287-020-01917-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510147PMC
September 2020

Genome-wide identification of meiotic recombination hot spots detected by SLAF in peanut (Arachis hypogaea L.).

Sci Rep 2020 08 14;10(1):13792. Epub 2020 Aug 14.

Key Laboratory of Peanut Biology and Genetic Improvement, Ministry of Agriculture, Shandong Peanut Research Institute, No.126, Wannianquan Road, Licang District, Qingdao, 266100, China.

Recombination hot spots (RHP), caused by meiosis, are considered to play crucial roles in improvement and domestication of crop. Cultivated peanut is one of the most important rich-source of oil and protein crops. However, no direct scale of recombination events and RHP have been estimated for peanut. To examine the scale of recombination events and RHP in peanut, a RIL population with 200 lines and a natural population with 49 cultivars were evaluated. The precise integrated map comprises 4837 SLAF markers with genetic length of 2915.46 cM and density of 1.66 markers per cM in whole genome. An average of 30.0 crossover (2.06 cMMb) events was detected per RIL plant. The crossover events (CE) showed uneven distribution among B sub-genome (2.32) and A sub-genome (1.85). There were 4.34% and 7.86% of the genome contained large numbers of CE (> 50 cMMb) along chromosomes in F and natural population, respectively. High density of CE regions called RHP, showed negative relationship to marker haplotypes conservative region but positive to heatmap of recombination. The genes located within the RHP regions by GO categories showed the responding of environmental stimuli, which suggested that recombination plays a crucial role in peanut adaptation to changing environments.
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http://dx.doi.org/10.1038/s41598-020-70354-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429841PMC
August 2020

[Application of convalescent plasma for the treatment of adult patients with coronavirus disease 2019].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Jun;32(6):646-651

Department of Critical Care Medicine, Peking Friendship Hospital, Beijing 100050, China. Corresponding author: Zhao Hong, Email:

The high incidence of coronavirus disease 2019 (COVID-19) and high mortality of critical patients have posed a great challenge to global public health resources. Currently there are no specific antiviral drugs and vaccines available for COVID-19, which has drawn the attention to the usefulness of convalescent plasma (CP) again, so the application of CP in the adult patients with COVID-19 is reviewed. The main contents include the possible mechanism of CP, the evidence of CP in the treatment of COVID-19 patients, the safety of clinical application of CP and the main factors affecting the clinical effect of CP, which may provide some basis for clinicians to choose CP for the treatment of adult patients with COVID-19.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200601-00479DOI Listing
June 2020

Myricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β.

Biochem Pharmacol 2020 08 11;178:114097. Epub 2020 Jun 11.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China; Tianjin Key Laboratory of Molecular Drug Research, Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, People's Republic of China. Electronic address:

Idiopathic pulmonary fibrosis is a progressive-fibrosing lung disease with high mortality and limited therapy, which characterized by myofibroblasts proliferation and extracellular matrix deposition. Myricetin, a natural flavonoid, has been shown to possess a variety of biological characteristics including anti-inflammatory and anti-tumor. In this study we explored the potential effect and mechanisms of myricetin on pulmonary fibrosis in vivo and vitro. The in vivo studies showed that myricetin effectively alleviated bleomycin (BLM)-induced pulmonary fibrosis. KEGG analysis of RNA-seq data indicated that myricetin could regulate the transforming growth factor (TGF)-β signaling pathway. In vitro studies indicated that myricetin could dose-dependently suppress TGF-β1/Smad signaling and attenuate TGF-β1-induced fibroblast activation and epithelial-mesenchymal transition (EMT). Molecular docking indicated that heat shock protein (HSP) 90β may be a potential target of myricetin, and MST assay demonstrated that the dissociation constant (Kd) of myricetin and HSP90β was 331.59 nM. We demonstrated that myricetin interfered with the binding of HSP90β and TGF-β receptor II and impeded fibroblast activation and EMT. In conclusion, myricetin impedes TGF-β1-induced lung fibroblast activation and EMT via targeting HSP90β and attenuates BLM-induced pulmonary fibrosis in mice.
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http://dx.doi.org/10.1016/j.bcp.2020.114097DOI Listing
August 2020

Thymidine phosphorylase promotes angiogenesis and tumour growth in intrahepatic cholangiocarcinoma.

Cell Biochem Funct 2020 Aug 31;38(6):743-752. Epub 2020 May 31.

Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical University, Jinan, People's Republic of China.

Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer, and thymidine phosphorylase (TP) is a regulator of angiogenesis. To investigate the biological activities of TP in ICC, we established human cholangiocarcinoma RBE cell lines overexpressing TP or silencing TP. Overexpression of TP enhanced viability, suppressed apoptosis and increased tube formation in human umbilical vein endothelial cells, while downregulation of TP reversed these effects. Moreover, an orthotopic xenograft mouse model of ICC was built to further explore TP's function in ICC in vivo. Histological analysis using H&E, TUNEL and Ki67 staining showed that TP promoted tumour growth and inhibited cell apoptosis. Immunostaining for CD31 revealed an elevation in microvessel density in the presence of TP. Besides, upregulation of TP increased the expression of vascular endothelial growth factor, basic fibroblast growth factor, interleukin-8 and tumour necrosis factor alpha. In contrast, TP knockdown inhibited tumour growth, suppressed microvessel formation and decreased the expression of angiogenesis-related proteins. Therefore, we suggest that TP promotes angiogenesis and tumour growth in ICC, which can be a potent therapeutic target for ICC treatment.
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http://dx.doi.org/10.1002/cbf.3541DOI Listing
August 2020

Predictors of 1-year mortality in patients on prolonged mechanical ventilation after surgery in intensive care unit: a multicenter, retrospective cohort study.

BMC Anesthesiol 2020 02 21;20(1):44. Epub 2020 Feb 21.

Department of Critical Care Medicine, Peking University First Hospital, Beijing, 100034, China.

Objectives: The requirement of prolonged mechanical ventilation (PMV) is associated with increased medical care demand and expenses, high early and long-term mortality, and worse life quality. However, no study has assessed the prognostic factors associated with 1-year mortality among PMV patients, not less than 21 days after surgery. This study analyzed the predictors of 1-year mortality in patients requiring PMV in intensive care units (ICUs) after surgery.

Methods: In this multicenter, respective cohort study, 124 patients who required PMV after surgery in the ICUs of five tertiary hospitals in Beijing between January 2007 and June 2016 were enrolled. The primary outcome was the duration of survival within 1 year. Predictors of 1-year mortality were identified with a multivariable Cox proportional hazard model. The predictive effect of the ProVent score was also validated.

Results: Of the 124 patients enrolled, the cumulative 1-year mortality was 74.2% (92/124). From the multivariable Cox proportional hazard analysis, cancer diagnosis (hazard ratio [HR] 2.14, 95% confidence interval [CI] 1.37-3.35; P < 0.01), no tracheostomy (HR 2.01, 95% CI 1.22-3.30; P < 0.01), enteral nutrition intolerance (HR 1.88, 95% CI 1.19-2.97; P = 0.01), blood platelet count ≤150 × 10/L (HR 1.77, 95% CI 1.14-2.75; P = 0.01), requirement of vasopressors (HR 1.78, 95% CI 1.13-2.80; P = 0.02), and renal replacement therapy (HR 1.71, 95% CI 1.01-2.91; P = 0.047) on the 21st day of mechanical ventilation (MV) were associated with shortened 1-year survival.

Conclusions: For patients who required PMV after surgery, cancer diagnosis, no tracheostomy, enteral nutrition intolerance, blood platelet count ≤150 × 10/L, vasopressor requirement, and renal replacement therapy on the 21st day of MV were associated with shortened 1-year survival. The prognosis in PMV patients in ICUs can facilitate the decision-making process of physicians and patients' family members on treatment schedule.
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http://dx.doi.org/10.1186/s12871-020-0942-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033944PMC
February 2020

Development of a Population Pharmacokinetic Model of Vancomycin and its Application in Chinese Geriatric Patients with Pulmonary Infections.

Eur J Drug Metab Pharmacokinet 2019 Jun;44(3):361-370

Department of Nephrology, Peking University First Hospital, No.1 Dahongluochang Street, Xicheng District, Beijing, China.

Background: The optimal use of vancomycin in the elderly requires information about the drug's pharmacokinetics and the influence of various factors on the drug's disposition. However, because of sampling restrictions, it is often difficult to perform traditional pharmacokinetic studies in elderly patients.

Objective: This study was conducted to estimate the population pharmacokinetics of vancomycin in Chinese geriatric patients (age ≥ 65 years) with pulmonary infections and to explore the clinical application of this information for vancomycin dose individualization.

Methods: The steady-state trough concentrations were retrospectively collected from January 2011 to December 2016 and were analyzed using the nonlinear mixed-effect model software. The final model was evaluated using the bootstrap method, goodness-of-fit plots and the normalized prediction distribution error method.

Main Outcome Measure: Model parameters and prediction error.

Results: A total of 125 steady-state trough concentrations from 70 patients were retrospectively collected. A one-compartment model was established. The final model was depicted as clearance (CL) [L/h] = 2.45 × (CL/56.28) × 0.542; volume of distribution (V) [L] = 154. The creatinine clearance (CL) was identified as the most significant covariate in the final model. The typical values of CL and V in the final model were 2.45 L/h and 154 L, respectively. Model validation outcomes showed that the final model was stable and had satisfactory prediction performance.

Conclusion: A population pharmacokinetic model was established to estimate the pharmacokinetics characteristics of Chinese geriatric patients with pulmonary infections, and this model can be used to develop an initial vancomycin dosing regimen for geriatric patients.
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http://dx.doi.org/10.1007/s13318-018-0534-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520475PMC
June 2019

Genomic and Transcriptomic Analysis Identified Gene Clusters and Candidate Genes for Oil Content in Peanut (Arachis hypogaea L.).

Plant Mol Biol Report 2018 23;36(3):518-529. Epub 2018 Jun 23.

1Key Laboratory for Peanut Biology, Genetics and Breeding, Ministry of Agriculture, Shandong Peanut Research Institute, Qingdao, 266100 China.

Peanut (), a major source of vegetable oil in many Asian countries, has become an integral part of human diet globally due to its high nutritional properties and option to consume in different forms. In order to meet the demand of vegetable oil, many peanut breeding programs of China have intensified their efforts in increasing oil content in newly bred varieties for reducing the import of edible oils in China. In this context, transcriptome sequencing data generated on 49 peanut cultivars were analyzed to identify candidate genes and develop molecular markers for seed oil content across multiple environments. Transcriptome analysis identified 5458 differentially expressed genes (DEGs) including 2243 positive DEGs and 3215 negative DEGs involved in oil synthesis process. Genome-wide association study identified 48 significant insertion/deletion (InDel) markers associated with seed oil content across five environments. A comparative genomics and transcriptomics analysis detected a total of 147 common gene clusters located in 17 chromosomes. Interestingly, an InDel cluster associated with seed oil content on A03 chromosome was detected in three different environments. Candidate genes identified on A03 form a haplotype, in which variable alleles were found to be different in oil content in an independent population. This locus is important for understanding the genetic control of peanut oil content and may be useful for marker-assisted selection in peanut breeding programs.
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http://dx.doi.org/10.1007/s11105-018-1088-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061501PMC
June 2018

Pregnancy-Related ICU Admissions From 2008 to 2016 in China: A First Multicenter Report.

Crit Care Med 2018 10;46(10):e1002-e1009

Department of Intensive Care Unit, Capital Medical University Affiliated Beijing Chao-Yang Hospital, Beijing, China.

Objectives: To identify the key points for improving severe maternal morbidity by analyzing pregnancy-related ICU admissions in Beijing.

Design: This was a retrospective, multicenter cohort study.

Setting: Three ICUs in tertiary hospitals in Beijing.

Patients: A total of 491 severe maternal cases in any trimester of pregnancy or within 42 days of delivery were reviewed between January 1, 2008, and December 31, 2016.

Interventions: None.

Measurements And Main Results: Among 491 obstetric ICU admissions (median Sequential Organ Failure Assessment score, 2) out of 87,850 hospital deliveries (a frequency of 5.6 admissions per 1,000 deliveries), the leading diagnoses were postpartum hemorrhage (170; 34.62%), hypertensive disorders of pregnancy (156; 31.77%), and cardio-cerebrovascular diseases (78; 15.9%). Comparing 2008-2011 to 2012-2016, the rates of maternal mortality (2.5% vs 1.9%; p = 0.991) and fetal loss (8.5% vs 8.6%; p = 0.977) did not decrease significantly, whereas the rates of ICU admission (3.05% vs 7.85%; p trends < 0.001) and postpartum hemorrhage (23% vs 38.5%; p = 0.002) increased. Hypertensive disorder (150/156; 96.2% transferred to the ICU postpartum, 24/28 women with fetal loss transferred from lower-level hospitals) was an independent maternal factor associated with fetal loss, and infections were the leading cause of maternal death (6/10) in the ICU.

Conclusions: Our study highlights the increasing rate of intensive care admissions for postpartum hemorrhage. Improving prenatal care quality for pregnancy-induced hypertension and sepsis at lower-level hospitals may improve maternal and fetal outcomes. Specifically, providing more effective regional cooperation before transfer and shifting patients who require continuous surveillance but not necessarily intensive care to a transitional ward in a tertiary hospital would provide more ICU beds for more prenatal intensive care for the most complex medical conditions.
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http://dx.doi.org/10.1097/CCM.0000000000003355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147102PMC
October 2018

[The progress of light sedation for critically ill adult patients in intensive care unit].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2016 Jan;28(1):89-93

Department of Critical Care Medicine, Peking University First Hospital, Beijing 100034, China (Li SL, Wang DX); Department of Anesthesiology , Peking University People's Hospital, Beijing 100044, China (Yang BX). Corresponding author: Li Shuangling, Email:

Objective: The latest advance of sedation for critically ill adult patients in intensive care unit (ICU) was reviewed in order to provide certain clinical information for the ICU physicians about sedation. Guidelines, clinical research, Meta-analysis, and reviews in recent years were collected using electronic data base. Discussions included: (1) the definition of light sedation, and its effects on clinical outcome, stress, sleep and delirium; (2) light sedation strategies included: the target population, the target sedation strategy and daily sedation interruption, clinical assessment and monitoring of sedation, selection of sedative drugs, light sedation extenuation; (3) light sedation strategies and pain, agitation, delirium control bundles; (4) the problems and prospects of light sedation. Light sedation is the main principle of currently ICU sedation strategy in critically ill adult patients. Goal-directed light sedation should be considered as a routine therapy in most clinical situation, and its goal should be achieved as early as possible in the early stage of sedation. Routine use of benzodiazepines should be avoided, especially in patients with or at a risk of delirium. Prevention and treatment of agitation with a combination of non-pharmacologic or pharmacologic methods; ICU specification rules for pain, agitation and delirium prevention and treatment should be made. Light sedation is the main ICU sedation strategy in adult patients now, but must be individualized for each patient.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2016.01.019DOI Listing
January 2016

Insulin-like growth factor 1 promotes growth of gastric cancer by inhibiting foxo1 nuclear retention.

Tumour Biol 2015 Jun 18;36(6):4519-23. Epub 2015 Jan 18.

Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan, 250014, China.

Gastric cancer (GC) is the fourth most common malignant human cancer. So far, the molecular mechanisms underlying the tumorigenesis of GC are not completely understood. Here, we reported significantly higher levels of serum insulin-like growth factor (IGF)-1 in GC patients and significantly higher levels of phosphorylated IGF-1 receptor (IGF-1R) in the GC specimen. Moreover, IGF-1 induced phosphorylation of IGF-1R and then phosphorylation of its downstream factor Akt in the GC cells. Further, IGF-1/Akt-induced forkhead box protein O1 (FoxO1) nuclear exclusion, but not IGF-1/Akt-induced mTOR phosphorylation, was essential for the augment in GC cell growth. Together, IGF-1/Akt/FoxO1 regulatory machinery appears to be a previously unappreciated signaling axis involved in the carcinogenesis of GC.
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http://dx.doi.org/10.1007/s13277-015-3096-9DOI Listing
June 2015

[The clinical monitoring of invasive aspergillosis in surgical intensive care unit during 10 years].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2014 Sep;26(9):634-8

Department of Surgical Intensive Care Unit, Peking University First Hospital, Beijing 100034, China, Corresponding author: Li Shuangling, Email:

Objective: To investigate the invasive aspergillosis (IA) status in a surgical intensive care unit (SICU).

Methods: The clinical data including general state, operation, pathogenic microorganisms, infection position, clinical situation, treatment and prognosis of patients with IA admitted to the SICU of Peking University First Hospital from January 2004 to December 2013 were retrospectively analyzed.

Results: 8 220 patients admitted to the SICU of Peking University First Hospital from January 2004 to December 2013 were enrolled. Of 8 220 patients, there were 29 cases experienced IA, with an incidence of 0.35%, and the incidence of hospital onset of IA infection was 0.27% (22/8 220). The incidence of hospital onset of IA infection was accounted for 6.98% (22/315) of the incidence of hospital onset of infection in SICU in the same period. Compared with 2004-2008, in 2009-2013, the incidence of hospital onset of infection was significantly decreased [3.19% (137/4 293) vs. 4.53% (178/3 927), χ² = 10.020, P=0.002], while the incidence of IA [0.56% (24/4 293) vs. 0.13% (5/3 927), χ² = 10.874, P=0.001], the incidence of hospital onset of IA infection [0.40% (17/4 293) vs. 0.13% (5/3 927), χ² = 5.556, P=0.019], and the percentage of incidence of hospital onset of IA infection according to the incidence of hospital onset of infection were increased by 5 years [12.40% (17/137) vs. 2.81% (5/178), χ² = 10.982, P=0.001]. Of 29 patients with IA, 25 cases had occurred after operation, and the majority of them were from the Department of General Surgery (13 cases), and followed by post-renal transplantation (6 cases) and post-thoracic surgery (3 cases). In the total submission of 155 specimens from 29 patients with IA, there were 17 strains isolated aspergillosis, among which included 2 strains of Aspergillus fumigatus, and 15 other un-subgrouped strains. The most common infection site was lower respiratory tract (23 cases, 79.31%). Sixteen patients were found with positive galactomannan (GM) test. In all the risk factors contributing to IA, the ratio of the long-term usage of broad-spectrum antibiotics over 4 days was the highest [36.25% (29/80)], which followed by the long-term use of hormone [18.75% (15/80)], complicated with acute kidney injury [18.75% (15/80)], liver injury [13.75% (11/80)], the long-term use of immunosuppressive orally [7.50% (6/18)], and complicated with neutropenia [5.00% (4/80)]. In 29 patients with IA, there were 28 patients received anti-fungal treatment except for 1 patient without treatment, and those were single use of itraconazole in 1 case, single use of echinocandins in 3 cases, single use of liposomal amphotericin B in 5 cases, 8 cases with voriconazole, single use of liposomal amphotericin B or echinocandins then replaced by voriconazole in 8 cases, and 3 cases of echinocandins therapy combined with voriconazole. Seventeen of 29 patients died, with a mortality rate of 58.62%.

Conclusions: IA is an uncommon but increasing postoperative complication of patients in SICU in recent 5 years. The most common sites of IA were lower respiratory tract. The mortality of IA is very high. So clinicians should pay more attention to the careful monitor for IA.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2014.09.006DOI Listing
September 2014