Publications by authors named "Shuanghong Yang"

8 Publications

  • Page 1 of 1

Lysozyme-like Protein Produced by Regulates Human Gut Microbiota Using In Vitro Models.

Molecules 2021 Oct 27;26(21). Epub 2021 Oct 27.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

The extracellular secreted protein of () plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial peptide. In this study, the amino acid sequence of a lysozyme-like protein of based on whole-genome data of an isolate from human gut feces was found. We further predicted functional domains from the amino acid sequence, purified the protein, and verified its bioactivity. The growth of some bacteria were significantly delayed by the 020402_LYZ M1 protein. In addition, the gut microbiota was analyzed via high-throughput sequencing of 16S rRNA genes and an in vitro fermentation model, and the fluctuations in the gut microbiota under the treatment of 020402_LYZ M1 protein were characterized. The 020402_LYZ M1 protein affected the composition of human gut microbiota significantly, implying that the protein is able to communicate with intestinal microbes as a regulatory factor.
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http://dx.doi.org/10.3390/molecules26216480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587964PMC
October 2021

Isolation and Characterization of a Novel Phage vB_SalP_TR2.

Front Microbiol 2021 21;12:664810. Epub 2021 Jun 21.

Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.

is a widely distributed foodborne pathogen. The use of phages as biocontrol agents has recently gained significant interest. Because the genus has high diversity, efforts are necessary to identify lytic phages focusing on different serovars. Here, five phages were isolated from soil samples, and vB_SalP_TR2 was selected as a novel phage with high lytic potential against the host serovar Albany, as well as other tested serovars, including Corvallis, Newport, Kottbus, and Istanbul. Morphological analyses demonstrated that phage vB_SalP_TR2 belongs to the family, with an icosahedral head (62 ± 0.5 nm in diameter and 60 ± 1 nm in length) and a short tail (35 ± 1 nm in length). The latent period and burst size of phage vB_SalP_TR2 was 15 min and 211 PFU/cell, respectively. It contained a linear dsDNA of 71,453 bp, and G + C content was 40.64%. Among 96 putative open reading frames detected, only 35 gene products were found in database searches, with no virulence or antibiotic resistance genes being identified. As a biological control agent, phage vB_SalP_TR2 exhibited a high temperature and pH tolerance. , it lysed most Albany after 24 h at 37°C with multiplicities of infection of 0.0001, 0.001, 0.01, 0.1, 1, 10, and 100. In food matrices (milk and chicken meat), treatment with phage vB_SalP_TR2 also reduced the number of Albany compared with that in controls. These findings highlighted phage vB_SalP_TR2 as a potential antibacterial agent for the control of in food samples.
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http://dx.doi.org/10.3389/fmicb.2021.664810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256156PMC
June 2021

Evaluation of the Cholesterol-Lowering Mechanism of Strain 132 and Strain 201 in Hypercholesterolemia Rats.

Nutrients 2021 Jun 9;13(6). Epub 2021 Jun 9.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

Hypercholesterolemia can cause many diseases, but it can effectively regulated by . This study aimed to evaluate the cholesterol-lowering mechanism of strain 132 and strain 201. These results showed that both the strains decreased serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), liver TC and TG and increased fecal TC, TG and total bile acid (TBA) levels. Additionally, both strains also reduced glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST) and levels of tissue inflammation levels to improve the lipid profile, and they reduced fat accumulation partially by alleviating inflammatory responses. Furthermore, both strains regulated the expression of the , , and gene to promote cholesterol metabolism and reduce TG accumulation. Interventions with both strains also altered the gut microbiota, and decreasing the abundance of Veillonellaceae, Erysipelotrichaceae and . Furthermore, fecal acetic acid and propionic acid were increased by this intervention. Overall, the results suggested that strain 132 and strain 201 can alleviate hypercholesterolemia in rats and might be applied as a new type of hypercholesterolemia agent in functional foods.
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http://dx.doi.org/10.3390/nu13061982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228983PMC
June 2021

Selective Isolation of From Human Faeces Using Pangenomics, Metagenomics, and Enzymology.

Front Microbiol 2021 21;12:649698. Epub 2021 Apr 21.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.

, an important genus for human health, is difficult to isolate. We applied metagenomics, pangenomics, and enzymology to determine the dominant glycoside hydrolase (GH) families of Bifidobacterium and designed selective medium for isolation. Pangenomics results showed that the GH13, GH3, GH42, and GH43 families were highly conserved in . Metagenomic analysis of GH families in human faecal samples was performed. The results indicated that contains core GHs for utilizing raffinose, D-trehalose anhydrous, D(+)-cellobiose, melibiose, lactulose, lactose, D(+)-sucrose, resistant starch, pullulan, xylan, and glucan. These carbohydrates as the main carbon sources were applied for selective media, which were more conducive to the growth of bifidobacteria. In the medium with lactose, raffinose and xylan as the main carbon sources, the ratio of cultivable bifidobacteria to cultivable microorganisms were 89.39% ± 2.50%, 71.45% ± 0.99%, and 53.95% ± 1.22%, respectively, whereas the ratio in the ordinary Gifu anaerobic medium was only 17.90% ± 0.58%. Furthermore, the species significantly ( < 0.05) varied among samples from different individuals. Results suggested that xylan might be a prebiotic that benefits host health, and it is feasible to screen and isolate bifidobacteria using the oligosaccharides corresponding to the specific GHs of bifidobacteria as the carbon sources of the selective media.
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http://dx.doi.org/10.3389/fmicb.2021.649698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096985PMC
April 2021

Significant decreases in the volatile organic compound concentration, atmospheric oxidation capacity and photochemical reactivity during the National Day holiday over a suburban site in the North China Plain.

Environ Pollut 2020 Aug 25;263(Pt A):114657. Epub 2020 Apr 25.

Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China; University of the Chinese Academy of Sciences, Beijing, 100049, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. Electronic address:

To what extent anthropogenic emissions could influence volatile organic compound (VOCs) concentrations and related atmospheric reactivity is still poorly understood. China's 70th National Day holidays, during which anthropogenic emissions were significantly reduced to ensure good air quality on Anniversary Day, provides a unique opportunity to investigate these processes. Atmospheric oxidation capacity (AOC), OH reactivity, secondary transformation, O formation and VOCs-PM sensitivity are evaluated based on parameterization methods and simultaneous measurements of VOCs, O, NO, CO, SO, PM, JOD, JNO, JNO carried out at a suburban site between Beijing and Tianjin before, during, and after the National Day holiday 2019. During the National Day holidays, the AOC, OH reactivity, O formation potential (OFP) and secondary organic aerosol formation potential (SOAP) were 1.6 × 10 molecules cm s, 41.8 s, 299.2 μg cm and 1471.8 μg cm, respectively, which were 42%, 29%, 47% and 42% lower than pre-National Day values and -12%, 42%, 36% and 42% lower than post-National Day values, respectively. Reactions involving OH radicals dominated the AOC during the day, but OH radicals and O reactions at night. Alkanes (the degree of unsaturation = 0, (D, Equation (1)) accounted for the largest contributions to the total VOCs concentration, oxygenated VOCs (OVOCs; D ≤ 1) to OH reactivity and OFP, and aromatics (D = 4) to the SOAP. O production was identified as VOCs-limited by VOCs (ppbC)/NOx (ppbv) ratios during the sampling campaign, with greater VOCs limitation during post- National Day and more-aged air masses during the National Day. The VOCs-sensitivity coefficient (VOCs-S) suggested that VOCs were more sensitive to PM in low-pollution domains and during the National Day holiday. This study emphasizes the importance of not only the abundance, reactivity, and secondary transformation of VOCs but also the effects of VOCs on PM for the development of effective control strategies to minimize O and PM pollution.
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http://dx.doi.org/10.1016/j.envpol.2020.114657DOI Listing
August 2020

Insights into the chemistry of aerosol growth in Beijing: Implication of fine particle episode formation during wintertime.

Chemosphere 2021 Jul 24;274:129776. Epub 2021 Jan 24.

State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry (LAPC), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Science, Xiamen, 361021, China; University of Chinese Academy of Science, Beijing, 100049, China.

Nucleation particle growth plays a major role in the occurrence of fine particles, yet the mechanism of new particle formation (NPF) remains ambiguous in the complex atmosphere of megacities and hinders the development of measures to mitigate PM pollution. In this study, the chemistry of ultrafine particles during the growth phase of nucleation events was investigated in urban Beijing from Nov. 15, 2018 to Jan. 15, 2019, using two scanning mobility particle spectrometers (SMPS) systems and an Aerodyne high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS). During this intense campaign, 11 NPF events were observed and the growth rate (GR) of nanoparticles ranged from 12.5 to 24.5 nm h. Four periodic cycles of PM episodes that included aerosol particle growth to particulate matter pollution were identified. Based on the Q - Q theoretical frame that exploring the balance between the source rate of condensable vapors and the observed growth rate of nanoparticles, we clearly showed the physical and chemical evolution of nano-particle during the growth processes to ambient-atmosphere sizes (>100 nm). Generally, the modal diameter of aerosol particles grew by more than 100 nm (7 out of 11 NPF events) when the nitrate concentration and less-oxidized oxygenated organic aerosol (LO-OOA) were high; however, another class of aerosol particle growth was limited to 50-100 nm (3 out of 11 NPF events) when sulfate was high. Note that the remaining one NPF event could not be identified if it can grow up to 100 nm or not due to the unavailable of observation data during the late growth stage. By linking the aerosol growth with chemical compositions, sulfate and organics were found to be the main contributors during the initial stage of the aerosol growth, while cooking-related OA (COA) enhanced the transition stage, and nitrate and more-oxidized OOA (MO-OOA) dominated the subsequent growth of aerosol to ambient-atmosphere sizes. An important portion of aerosol growth in PM was controlled by semi-volatile organic vapors, which can partition into the externally condensed phase of the accumulation mode and coarse mode via the physical process of adsorption. Through quantifying the physical and chemical properties of aerosol particle growth, the detail processes of nucleation initiated PM pollution episodes were evaluated and provided observational evidence on the formation mechanism of winter haze pollution in the megacity of Beijing.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129776DOI Listing
July 2021

Probiotics supplementation improves hyperglycemia, hypercholesterolemia, and hypertension in type 2 diabetes mellitus: An update of meta-analysis.

Crit Rev Food Sci Nutr 2021 21;61(10):1670-1688. Epub 2020 May 21.

School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi an, China.

Although many studies have shown that consumption of probiotics is relevant to diabetes, the effects of probiotics improves clinical outcomes in type 2 diabetes have yielded conflicting results. The aim of this meta-analysis was conducted to assess the effects of probiotics supplementation on glycemic, blood lipids, pressure and inflammatory control in type 2 diabetes. PubMed, Web of science, Embase and the Cochrane Library databases were searched for relevant studies from February 2015 up to Janurary 2020, with no language restrictions. The pooled results were calculated with the use of a random-effects model to assess the impact of supplemental probiotics on glycemic, blood lipids, pressure and inflammatory control in type 2 diabetes. Additionally, subgroup analysis was conducted based on patients age, body mass index (BMI), country and duration of the probiotics supplement, respectively. 13 studies were included in this meta-analysis, involving a total of 818 participants in 8 countries. Overall, compared with control groups, the subjects who received multiple species of probiotics had a statistically significant reduction in fasting blood sugar (FBS), homeostasis model assessment of insulin resistance (HOMA-IR), total cholesterol (TC), triglycerides (TG), systolic blood pressure (SBP), diastolic blood pressure (DBP) and tumor necrosis factor (TNF) -α [standardized mean difference (SMD): -0.89 mg/Dl, 95% CI: -1.66, -0.12 mg/dL; SMD: -0.43, 95% CI: -0.63, -0.23; SMD: -0.19 mg/dL, 95% CI: -0.36, -0.01 mg/dL; SMD: -0.23 mg/dL, 95% CI: -0.40, -0.05 mg/dL; SMD: -5.61 mmHg, 95% CI: -9.78, -1.45 mmHg; SMD: -3.41 mmHg, 95% CI: -6.12, -0.69 mmHg; and SMD: 6.92 pg/ml, 95% CI: 5.95, 7.89 pg/ml, respectively]. However, the subjects who received single-species of probiotic or probiotic with co-supplements in food only changed FBS, HOMA-IR, DBP and TG levels. Moreover, subgroup analyses revealed that the effects of probiotics supplementation on FBS, HMOA-IR, SBP and DBP are significantly influenced by patients age, body mass index (BMI), country and duration of the probiotics supplement. Our analysis revealed that glycemic, lipids, blood pressure and inflammation indicators are significantly improved by probiotic supplementation, particularly the subjects who ages ≤ 55, baseline BMI< 30 kg/m, duration of intervention more than 8 weeks, and received multiple species probiotic.
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http://dx.doi.org/10.1080/10408398.2020.1764488DOI Listing
April 2021

Characteristics of fine particle explosive growth events in Beijing, China: Seasonal variation, chemical evolution pattern and formation mechanism.

Sci Total Environ 2019 Oct 6;687:1073-1086. Epub 2019 Jun 6.

State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100191, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Fine particle explosive growth (FPEG) events are frequently observed in heavy haze episodes in Beijing, the characteristics and formation mechanism of which remain not fully understood. In this study, a five year (2013-2017) online observation was conducted in Beijing and the chemical evolution pattern of FPEG events was analyzed to understand its formation mechanism. A total of 132 FPEG events were identified, and steadily decreased from 39 events in 2013 to 19 events in 2017. More than 70% of the FPEG events occurred in winter and autumn, which coincides with adverse weather conditions and enhanced primary emissions. Organic matter (OM) was the dominated components (~30%) in PM, but it only accounted for 10% of total FPEG events as a driven factor, because its contribution usually decreased when the FPEG events developed. In contrast, the secondary inorganic species were the dominated driven factors, and sulfate-driven events accounted >50%. During the period of 2013-2017, the contribution from regional sources decreased significantly mainly due to the reduction of emissions from regional sources, while the contribution from local sources remained largely unchanged, indicating that the local secondary transformation played a leading role in promoting the FPEG events. The low nitrogen oxidation rates (NOR, 0.12 ± 0.07) and the weak increase trend of NOR with elevated RH were observed, indicating the formation of which might be promoted by the homogenous reaction between HNO and NH. In contrast, a significant increase in sulfur oxidation rate (SOR, 0.50 ± 0.19) was observed when RH > 50%, suggesting enhanced heterogeneous oxidation of SO in FPEG events. In addition, our analysis suggest the S (IV) heterogeneous oxidation rates in FPEG events depend mainly on the aerosol liquid water content (ALWC) in addition to the aerosol acidity. This study provides observational evidence for understanding the formation mechanism of FPEG events in Beijing.
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http://dx.doi.org/10.1016/j.scitotenv.2019.06.068DOI Listing
October 2019
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