Publications by authors named "Shuang Wu"

707 Publications

Treadmill training improves respiratory function in rats after spinal cord injury by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway.

Neurosci Lett 2022 May 17:136686. Epub 2022 May 17.

Guizhou Medical University, 9 Beijing Street, Yunyan District, Guiyang, Guizhou, China; Affiliated Hospital of Guizhou Medical University, 28 Guiyi Street, Yunyan District, Guiyang, Guizhou, China. Electronic address:

Objective: To investigate the effects of treadmill training on lung injury and HMGB1/TLR4/NF-κB after spinal cord injury (SCI) in rats.

Methods: A total of 108 female SD rats were randomly divided into three groups: sham operation group, SCI brake group, and SCI exercise group. The rats in the SCI exercise group began treadmill running training on the 3rd day after the operation. The rats in the SCI brake group underwent braking treatment. The lung tissues were obtained on the 3rd, 7th, and 14th days after exercise. Locomotor functional recovery was determined using the BBB scores and inclined plane test. Respiratory function was determined via abdominal aortic blood gas analysis. HE staining was used to detect pathological changes in rat lung tissue. RNA sequencing was used to identify differentially expressed genes at different phases in each group of lung tissues. HMGB1, TLR4, and NF-κB in lung tissue were detected using immunohistochemistry and immunofluorescence. Detection of HMGB1 levels in serum, spinal cord tissues and lung tissues by ELISA. HMGB1, TLR4, NF-κB, IL-1β, IL-6, TNF-α mRNA, and protein expression levels were detected via qRT PCR and western blot.

Results: Motor and respiratory functions significantly decreased after SCI (P<0.05). However, locomotion and respiratory functions were significantly improved after treadmill training intervention (P < 0.05). HE staining showed that interstitial thickening, inflammatory cells, and erythrocyte infiltration occurred in lung tissue of rats after SCI (P<0.05). Moreover, inflammatory reaction in lung tissue was significantly reduced after treadmill training intervention (P<0.05). A total of 428 differentially expressed mRNAs [(|log2(FC)| > 2, P < 0.05)] were identified in the intersection of the three groups. KEGG analysis identified five enriched signal pathways, including NF-kappa B. ELISA results showed that treadmill training could significantly reduce the levels of HMGB1 in serum, spinal cord tissue and lung tissue that were elevated after SCI (P < 0.05). Immunohistochemistry, immunofluorescence, qRT PCR, and Western blot showed that HMGB1, TLR4, IL-1β, IL-6, TNF-α, and NF-κB expressions were significantly up-regulated at the 3rd, 7th and 14th days after SCI, compared with the sham operation group. Besides, inflammatory cytokines were significantly lower in the SCI exercise group than in the SCI brake group at all time points after intervention (P < 0.05).

Conclusion: Treadmill training alleviates lung tissue inflammation and promotes recovery of motor and respiratory functions by inhibiting the HMGB1/TLR4/NF-κB signaling pathway after SCI in rats.
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http://dx.doi.org/10.1016/j.neulet.2022.136686DOI Listing
May 2022

Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery.

Plant Methods 2022 May 19;18(1):68. Epub 2022 May 19.

College of Resource Environment and Tourism, Capital Normal University, Beijing, 100048, China.

Background: Leaf Area Index (LAI) is half of the amount of leaf area per unit horizontal ground surface area. Consequently, accurate vegetation extraction in remote sensing imagery is critical for LAI estimation. However, most studies do not fully exploit the advantages of Unmanned Aerial Vehicle (UAV) imagery with high spatial resolution, such as not removing the background (soil and shadow, etc.). Furthermore, the advancement of multi-sensor synchronous observation and integration technology allows for the simultaneous collection of canopy spectral, structural, and thermal data, making it possible for data fusion.

Methods: To investigate the potential of high-resolution UAV imagery combined with multi-sensor data fusion in LAI estimation. High-resolution UAV imagery was obtained with a multi-sensor integrated MicaSense Altum camera to extract the wheat canopy's spectral, structural, and thermal features. After removing the soil background, all features were fused, and LAI was estimated using Random Forest and Support Vector Machine Regression.

Results: The results show that: (1) the soil background reduced the accuracy of the LAI prediction of wheat, and soil background could be effectively removed by taking advantage of high-resolution UAV imagery. After removing the soil background, the LAI prediction accuracy improved significantly, R raised by about 0.27, and RMSE fell by about 0.476. (2) The fusion of multi-sensor synchronous observation data could achieve better accuracy (R = 0.815 and RMSE = 1.023), compared with using only one data; (3) A simple LAI prediction method could be found, that is, after selecting a few features by machine learning, high prediction accuracy can be obtained only by simple multiple linear regression (R = 0.679 and RMSE = 1.231), providing inspiration for rapid and efficient LAI prediction of wheat.

Conclusions: The method of this study can be transferred to other sites with more extensive areas or similar agriculture structures, which will facilitate agricultural production and management.
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http://dx.doi.org/10.1186/s13007-022-00899-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118866PMC
May 2022

Identification and validation of the N6-methyladenosine RNA methylation regulator ZC3H13 as a novel prognostic marker and potential target for hepatocellular carcinoma.

Int J Med Sci 2022 21;19(4):618-630. Epub 2022 Mar 21.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, People's Republic of China.

N6-methyladenosine (m6A) RNA methylation has been implicated in various malignancies. This study aimed to identify prognostic signature based on m6A methylation regulators for hepatocellular carcinoma (HCC) and provide candidate targets for HCC treatment. In this study, the expression levels, prognostic values, correlation with tumor grades and genetic variations of m6A-related genes in HCC were evaluated using bioinformatics analyses. Interestingly, the results show that methyltransferase zinc finger CCCH-type containing 13 (ZC3H13) was expressed at a significantly low level in HCC. Survival outcome analysis suggested that significant correlations existed between ZC3H13 downregulation and poor overall survival (OS) and poor recurrence-free survival (RFS) in HCC patients. Therefore, ZC3H13 was chosen for further experimental validation. The expression of ZC3H13 in HCC cell lines was investigated by western blotting. Knockdown of ZC3H13 significantly enhanced the migration and invasion of HCC cells, as demonstrated by wound healing and transwell assays. Moreover, upregulating ZC3H13 repressed the growth of xenograft tumors . Functional and pathway enrichment analyses indicated that ZC3H13 might be involved in transcriptional dysregulation or the JAK-STAT signaling pathway in cancer. Additionally, ZC3H13 expression was significantly correlated with lymphocytes and immunomodulators. Therefore, ZC3H13 is a promising candidate as a novel biomarker and therapeutic target for HCC.
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http://dx.doi.org/10.7150/ijms.69645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108408PMC
May 2022

Sensory and autonomic innervation of the local tissues at traditional acupuncture point locations GB14, ST2 and ST6.

Acupunct Med 2022 May 17:9645284221085579. Epub 2022 May 17.

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China.

Objective: To visualize and compare the sensory and autonomic innervation of the local tissues at the sites of different traditional acupuncture points in the rat forehead and face by histochemical examination.

Methods: GB14 (), ST2 () and ST6 () were selected as the representative traditional acupuncture points in this study, and the local tissues at these sites were dissected in rats after perfusion followed by double or triple fluorescent histochemical staining. Here, calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH) and vesicular acetylcholine transporter (VAChT) were used to label the sensory, sympathetic and parasympathetic nerve fibers, respectively.

Results: The CGRP sensory, TH sympathetic and VAChT parasympathetic nerve fibers were simultaneously demonstrated in the local tissues at GB14, ST2 and ST6. Although the three kinds of nerve fibers ran in parallel or intermingled with each other, by the analysis from the view of three-dimensional reconstruction, it was clear that each of them distributed in an independent pattern to their corresponding target tissues including the blood vessels, hair follicles, arrector pili and subcutaneous muscles, as well as sebaceous glands.

Conclusion: Our study demonstrated the sensory and autonomic innervation of the local tissues at GB14, ST2 and ST6, providing neurochemical evidence indicating that the CGRP sensory, TH sympathetic and VAChT parasympathetic nerve fibers form a neural network at these point locations that may respond to acupuncture stimulation.
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http://dx.doi.org/10.1177/09645284221085579DOI Listing
May 2022

Global methane and nitrous oxide emissions from inland waters and estuaries.

Glob Chang Biol 2022 May 12. Epub 2022 May 12.

Key Laboratory of Low-carbon and Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Inland waters (rivers, reservoirs, lakes, ponds, streams) and estuaries are significant emitters of methane (CH ) and nitrous oxide (N O) to the atmosphere, while global estimates of these emissions have been hampered due to the lack of a worldwide comprehensive data set of CH and N O flux components. Here, we synthesize 2997 in-situ flux or concentration measurements of CH and N O from 277 peer-reviewed publications to estimate global CH and N O emissions from inland waters and estuaries. Inland waters including rivers, reservoirs, lakes, and streams together release 95.18 Tg CH  year (ebullition plus diffusion) and 1.48 Tg N O year (diffusion) to the atmosphere, yielding an overall CO -equivalent emission total of 3.06 Pg CO  year . The estimate of CH and N O emissions represents roughly 60% of CO emissions (5.13 Pg CO  year ) from these four inland aquatic systems, among which lakes act as the largest emitter for both CH and N O. Ebullition showed as a dominant flux component of CH , contributing up to 62%-84% of total CH fluxes across all inland waters. Chamber-derived CH emission rates are significantly greater than those determined by diffusion model-based methods for commonly capturing of both diffusive and ebullitive fluxes. Water dissolved oxygen (DO) showed as a dominant factor among all variables to influence both CH (diffusive and ebullitive) and N O fluxes from inland waters. Our study reveals a major oversight in regional and global CH budgets from inland waters, caused by neglecting the dominant role of ebullition pathways in those emissions. The estimated indirect N O EF values suggest that a downward refinement is required in current IPCC default EF values for inland waters and estuaries. Our findings further indicate that a comprehensive understanding of the magnitude and patterns of CH and N O emissions from inland waters and estuaries is essential in defining the way of how these aquatic systems will shape our climate.
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http://dx.doi.org/10.1111/gcb.16233DOI Listing
May 2022

Determination of 52 hidden chemical pesticides in biopesticide products by GC-MS/MS and LC-MS/MS.

J Environ Sci Health B 2022 May 13:1-12. Epub 2022 May 13.

Chongqing Academy of Agricultural Science, Chongqing, P.R. China.

Addition of chemical pesticides in biopesticides are expected to be common, due to effect of pest control during plant protection. These hidden chemical pesticides may cause various food safety problems if consumed. The purpose of our study was to develop a method to determine 52 hidden chemical pesticides in three formulation biopesticide products. Optimizations of different parameters, such as the instrument analysis condition for target, the extraction, and the composition of clean-up materials were carried out. The developed method used acetonitrile as an extraction solvent for all biopesticide formulations. The composition of adsorbents was based on QuEChERS, called QuSEL which was a needle filter head, containing PSA, GCB, and MgSO. Fifty-two chemical pesticides residues were then analyzed by GC-MS/MS and LC-MS/MS. The present results showed good linearity by correlation coefficients of more than 0.99 for all analyses. The LOQ ranged from 5.0 to 20.0 μg kg. Recoveries of 32 chemical pesticides ranged from 71.9% to 118.4% at the spiked level of 10, 50, and 100 μg kg, and 20 chemical pesticides ranged from 75.3% to 119.8% at the spiked level of 5, 25, and 50 μg kg. The developed method was applied for biopesticide products, and 9 samples were positive in 20 samples.
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http://dx.doi.org/10.1080/03601234.2022.2072645DOI Listing
May 2022

A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration.

Regen Biomater 2022 25;9:rbac015. Epub 2022 Apr 25.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu 610000, China.

Cell-based tissue engineering is one of the optimistic approaches to replace current treatments for bone defects. Urine-derived stem cells (USCs) are obtained non-invasively and become one of the promising seed cells for bone regeneration. An injectable BMP2-releasing chitosan microspheres/type I collagen hydrogel (BMP2-CSM/Col I hydrogel) was fabricated. USCs proliferated in a time-dependent fashion, spread with good extension and interconnected with each other in different hydrogels both for 2D and 3D models. BMP2 was released in a sustained mode for more than 28 days. Sustained-released BMP2 increased the ALP activities and mineral depositions of USCs in 2D culture, and enhanced the expression of osteogenic genes and proteins in 3D culture. , the mixture of USCs and BMP2-CSM/Col I hydrogels effectively enhanced bone regeneration, and the ratio of new bone volume to total bone volume was 38% after 8 weeks of implantation. Our results suggested that BMP2-CSM/Col I hydrogels promoted osteogenic differentiation of USCs in 2D and 3D culture and USCs provided a promising cell source for bone tissue engineering . As such, USCs-seeded hydrogel scaffolds are regarded as an alternative approach in the repair of bone defects.
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http://dx.doi.org/10.1093/rb/rbac015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070791PMC
April 2022

Henry's Law Constants and Indoor Partitioning of Microbial Volatile Organic Compounds.

Environ Sci Technol 2022 May 6. Epub 2022 May 6.

Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.

Microbial volatile organic compounds (MVOCs) play an essential role in many environmental fields, such as indoor air quality. Long-term exposure to odorous and toxic MVOCs can negatively affect the health of occupants. Recently, the involvement of surface reservoirs in indoor chemistry has been realized, which signifies the importance of the phase partitioning of volatile organic pollutants. However, reliable partition coefficients of many MVOCs are currently lacking. Equilibrium partition coefficients, such as Henry's law constant, , are crucial for understanding the environmental behavior of chemicals. This study aims to experimentally determine the values and their temperature dependence for key MVOCs under temperature relevant to the indoor environment. The values were determined with the inert gas-stripping (IGS) method and variable phase ratio headspace (VPR-HS) technique. A two-dimensional partitioning model was applied to predict the indoor phase distribution of MVOCs and potential exposure pathways to the residences. The findings show that the MVOCs are likely distributed between the gas and weakly polar (e.g., organic-rich) reservoirs indoors. Temperature and the volume of reservoirs can sensitively affect indoor partitioning. Our results give a more comprehensive view of indoor chemical partitioning and exposure.
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http://dx.doi.org/10.1021/acs.est.1c07882DOI Listing
May 2022

Theoretical and experimental study of the microstructure of a metallic melt in an InBi alloy based on the Wulff cluster model.

Phys Chem Chem Phys 2022 May 11;24(18):10798-10806. Epub 2022 May 11.

Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, Shandong University, Jinan 250061, People's Republic of China.

In this paper, the Wulff cluster model which has been proved to successfully describe the melt structure of pure metals, homogenous alloys and eutectic alloys has been extended to an alloy with intermetallic compounds (InBi). According to the cohesive energy and the solid-state XRD patterns, the most possible types of clusters in the melt are Bi and InBi. At relatively high temperatures, the superimposed XRD (simulated) patterns of Bi and InBi clusters are in good agreement with the experimental HTXRD patterns in terms of the position and intensity of the peaks. With the decrease of temperature, there is an obvious deviation in the simulated XRD value at the second peak caused by the nucleation process of Bi clusters, which would be modified by adding simulated XRD patterns of the Bi bulk. The proportion of the superimposed Bi bulk XRD pattern increases with the decrease of temperature suggesting that the nucleation process of the Bi cluster begins at 160 °C.
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http://dx.doi.org/10.1039/d2cp00674jDOI Listing
May 2022

Hook plate fixation with versus without coracoclavicular reconstruction for distal clavicular fractures.

J Orthop Surg (Hong Kong) 2022 Jan-Apr;30(1):10225536221088630

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, 34753Sichuan University, Chengdu, Sichuan, People's Republic of China.

Purpose: Hook plate fixation is one of the most frequently used methods for unstable distal clavicular fractures, but it is still unknown if there is a need for coracoclavicular (CC) reconstruction. This study aimed to compare the efficacy of hook plate fixation with versus without CC reconstruction for distal clavicular fractures.

Methods: Eighty-one patients who underwent hook plate fixation (HP group, = 45) or hook plate fixation plus suture anchor reconstruction (HPA group, = 36) for Neer type II or V clavicular fractures were enrolled. Demographics, fracture characteristics, and surgical data were recorded. Union time, coracoclavicular distance (CCD), post-operative complications, Constant score, and Disabilities of the Arm, Shoulder, and Hand (DASH) score were compared between HPA and HP groups.

Results: Constant score in the HPA group was higher than that in the HP group (91.8 ± 3.6 vs 88.8 ± 6.0, P = 0.007). However, there were no significant differences in union time, DASH score, CCD, and post-operative complications between the two groups (P > 0.05). Hook plate fixation combined with CC reconstruction costed more (3023.7 ± 202.6 vs 2416.2 ± 167.6 EUR, P < 0.001) and prolonged operative duration (78.2 ± 9.2 vs 73.7 ± 8.3 min, P = 0.023) compared with hook plate fixation alone.

Conclusion: Hook plate fixation with or without suture anchor reconstruction achieved satisfactory outcomes for Neer type II or V clavicular fractures. However, hook plate fixation plus CC reconstruction showed better functional outcomes compared with hook plate fixation alone.
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http://dx.doi.org/10.1177/10225536221088630DOI Listing
April 2022

Acute glycemic variability and risk of mortality in patients with sepsis: a meta-analysis.

Diabetol Metab Syndr 2022 Apr 23;14(1):59. Epub 2022 Apr 23.

Department of Infectious Diseases, Yiwu Central Hospital, No. 519 Nanmen Street, Yiwu, 322000, Zhejiang, China.

Background: Acute glycemic variability (GV) has been correlated with the severity of sepsis. The aim of the study was to evaluate the potential association between acute GV and mortality risk in patients with sepsis.

Methods: Cohort studies comparing the risk of death within 3 months between septic patients with higher versus lower acute GV were retrieved by systematic search of Medline, Embase, Web of Science, Wanfang and CNKI databases. We used a random-effect model to pool the data by incorporating the between-study heterogeneity. Sensitivity analyses were performed to evaluate the stability of the findings.

Results: Ten studies including 4296 patients were available for the meta-analysis. Pooled results showed that septic patients with higher acute GV had significantly increased mortality risk compared to those with lower acute GV, as evidenced by results using different parameters including standard deviation of blood glucose (SDBG, risk ratio [RR]: 1.74, 95% confidence interval [CI] 1.36-2.24, p < 0.001; I = 0%), coefficient of variation of blood glucose (RR: 1.91, 95% CI 1.57-2.31, p < 0.001; I = 0%), mean amplitude of glycemic excursion (RR: 1.81. 95% CI 1.36-2.40, p < 0.001; I = 0%), and glycemic lability index (RR: 2.52, 95% CI 1.72-3.68, p < 0.001; I = 0%). Sensitivity analyses by excluding one study at a time did not significantly affect the results (p all < 0.05).

Conclusions: Higher acute GV may be a predictor of mortality risk in patients with sepsis.
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http://dx.doi.org/10.1186/s13098-022-00819-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034073PMC
April 2022

Cancer and obstructive sleep apnea: An updated meta-analysis.

Medicine (Baltimore) 2022 Mar 11;101(10):e28930. Epub 2022 Mar 11.

Clinical Laboratory, The Affiliated Children Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Objective: The relationship between cancers and obstructive sleep apnea (OSA) has been discussed for decades. However, the previous meta-analysis led to opposite conclusions. To further investigate this controversial issue, we performed this systematic review and meta-analysis update.

Methods: PubMed, Embase, and the Cochrane Library were systematically searched and studies on "cancer and OSA" were all included. Two reviewers independently searched articles, extracted data, and assessed the quality of included studies. Moreover, the overall incidence of cancer and OSA in corresponding populations was calculated.

Results: Of the 1434 titles identified, 22 articles involving more than 32.1 million patients were included in this meta-analysis. An overall incidence of OSA positive individuals in cancer was 46 (95%CI, 27-67)%, and the prevalence of cancers in OSA patients reached 1.53 (95%CI, 1.01-2.31) times higher than non-OSA individuals.

Conclusion: This meta-analysis indicated that there was a high prevalence of OSA in cancer patients, and individuals with OSA were more likely to develop tumors, and the incidence was related to the severity of OSA.
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http://dx.doi.org/10.1097/MD.0000000000028930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913079PMC
March 2022

Transcriptome analysis of the growth-promoting effect of volatile organic compounds produced by Microbacterium aurantiacum GX14001 on tobacco (Nicotiana benthamiana).

BMC Plant Biol 2022 Apr 22;22(1):208. Epub 2022 Apr 22.

Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530008, China.

Background: Plant growth-promoting rhizobacteria (PGPR) release volatile organic compounds (VOCs), which promote plant growth.

Results: A potential PGPR strain GX14001 was isolated from marine samples, and the VOCs produced by GX14001 significantly promoted tobacco (Nicotiana benthamiana) growth in a plate experiment. Based on 16S rRNA sequence alignment and physiological and biochemical characterization, GX14001 was identified as Microbacterium aurantiacum. Comparative transcriptome analysis was conducted between GX14001 VOCs-treated tobacco and the control; it was found that 1286 genes were upregulated and 1088 genes were downregulated. Gene ontology (GO) analysis showed that upregulated genes were involved in three biological processes: polysaccharide metabolic, polysaccharide catabolic and carbohydrate metabolic. The downregulated genes were involved in six biological processes, namely cell redox homeostasis, cellular homeostasis, carbohydrate metabolic process, homeostatic process, obsolete electron transport, and regulation of biological quality. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that 190 upregulated differentially expressed genes were mainly involved in plant hormone signal transduction, phenylpropyl biosynthesis, plant-pathogen interaction, and flavonoid biosynthesis. The 148 downregulated differentially expressed genes were mainly involved in plant hormone signal transduction and the metabolism of ascorbic, aldehyde, and pyruvate acids. Further analysis revealed that many genes were differentially expressed in the metabolic pathways of plant hormone signals, which were speculated to be the main reason why GX14001 VOCs promoted tobacco growth. To further study its regulatory mechanism, we found that GX14001 promoted plant growth through auxin, salicylic acid, and gibberellin in Arabidopsis mutant experiments.

Conclusion: The VOCs produced by Microbacterium aurantiacum GX14001 may promote the growth of tobacco through the auxin, salicylic acid and gibberellin pathways.
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http://dx.doi.org/10.1186/s12870-022-03591-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028074PMC
April 2022

[Genetic analysis of a Chinese pedigree affected with branchiootic syndrome due to a nonsense variant of EYA1 gene].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2022 Apr;39(4):374-377

Department of Prenatal Diagnosis, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.

Objective: To analyze the clinical phenotype and genetic basis for a Chinese pedigree suspected for branchiootic syndrome (BOS).

Methods: The proband was subjected to target-capture high-throughput sequencing to detect potential variant of deafness-associated genes. Candidate variants were verified by Sanger sequencing of the family members.

Results: The proband was found to harbor a c.1627C>T (p.Gln543Ter) nonsense variant of the EYA1 gene. Sanger sequencing confirmed that all of the 4 patients with the BOS phenotype from the pedigree have harbored the same heterozygous variant. Based on the guidelines of the American College of Medical Genetics and Genomics, the variant was predicted to be pathogenic (PVS1+PS+PP3+PP4).

Conclusion: The c.1627C>T (p.Gln543Ter) variant of the EYA1 gene probably underlay the BOS phenotype in this pedigree. Above finding has provided a basis for its clinical diagnosis.
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http://dx.doi.org/10.3760/cma.j.cn511374-20201210-00866DOI Listing
April 2022

Potential Matrix Effects in Iodometry Determination of Peroxides Induced by Olefins.

J Phys Chem A 2022 May 20;126(17):2632-2644. Epub 2022 Apr 20.

Department of Chemistry, University of Alberta, Edmonton T6G 2G2, Alberta, Canada.

Peroxides (HO, ROOR, and ROOH) are an important reaction intermediate involved in a number of natural processes, including atmospheric autoxidation and lipid peroxidation in oils and animal tissues. Iodometry is an established spectroscopic technique that has been widely used to quantify total peroxide concentration in food, indoor, and outdoor samples. Iodometry provides selectivity toward peroxides through a quantitative reaction between I and peroxides to form I via a molecular iodine (I) intermediate. However, equilibrium changes caused by a potential interaction between olefinic species and I can suppress I formation, thereby underestimating peroxide concentration. For the first time in the current study, this unrecognized interference posed by olefins (OEs) is systematically investigated to gauge its effects on the accuracy of iodometry. A number of model molecules were investigated. The interference was observed to be unique to OEs, but universally affecting different peroxide species such as HO, -butyl hydroperoxide, and aerosol-bound peroxides. A simple kinetic box model was built to explain this chemistry. The measured rate constant for 3-octenoic acid was found to be 0.84 ± 0.02 M s. Overall, our results show matrix effects induced by OEs can underestimate peroxide concentration determined by iodometry for edible oils, indoor environments, and animal fat, but absent in most of the atmospheric samples. Nonetheless, our results point out the importance of this interfering chemistry in matrices enriched with OEs.
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http://dx.doi.org/10.1021/acs.jpca.1c10717DOI Listing
May 2022

Ball pen writing-without-ink: a truly simple and accessible method for sensitivity enhancement in lateral flow assays.

RSC Adv 2022 Jan 13;12(4):2068-2073. Epub 2022 Jan 13.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University Xi'an 710049 P. R. China

Lateral flow assays (LFAs), a popular point-of-care testing platform, have found widespread applications from laboratory to clinics. However, LFA-based testing is still subject to limited detection sensitivity, especially for classical gold nanoparticle-based LFAs. Inspired by traditional pen-based writing technologies, we developed a ball pen writing-without-ink method to amplify the detection signal of LFAs through controlling fluid flow rate. An enhancement of detection sensitivity by two times was obtained. Since the underlying mechanism of this method to improve detection sensitivity is to control the flow rate of the liquid on paper, it may be suitable for most paper-based platforms.
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http://dx.doi.org/10.1039/d1ra07684aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979190PMC
January 2022

Mediator complex subunit 8 is a prognostic biomarker in hepatocellular carcinoma.

Am J Transl Res 2022 15;14(3):1765-1777. Epub 2022 Mar 15.

Department of Anesthesiology, Rizhao International Heart Hospital Rizhao, Shandong, China.

Background: Mediator complex subunit 8 () is known for its role in encoding a subunit of the mediator complex (), that is critical for transcription. MED8 is significantly expressed in various tumors and has been correlated with an unfavorable prognosis. Nevertheless, no relationships have been found between MED8 and the clinical characteristics of hepatocellular carcinoma (HCC).

Methods: To conduct an evaluation of correlations between clinicopathologic characteristics and MED8 expression, the logistic regression, Wilcoxon signed-rank test, and Kruskal-Wallis test were used. To perform analysis of factors contributing to prognosis, the Kaplan-Meier approach and the Cox regression analyses were used. A nomogram on the basis of a Cox multivariate analysis was employed to anticipate the influence of MED8 on patient prognosis. The receiver operating characteristic (ROC) curves were plotted and the areas under the curve (AUC) were calculated to assess the prognostic value of MED8. Both immune infiltration analysis and Gene Set Enrichment Analysis (GSEA) were applied to reveal significant enrichment differences among TCGA data. Quantitative RT-PCR (qRT-PCR) and western blotting were used to verify the difference in the expression of MED8 in normal and hepatocellular carcinoma cells. The immunohistochemical method was used to validate the MED8 expression in tumor and adjoining tissues of HCC patients.

Results: A univariate analysis showed that high MED8 expression predicts poor disease-specific survival (DSS) (HR: 2.57; 95% confidence interval (CI) 1.62, 4.07; P<0.001). Multivariate regression analysis showed that high MED8 (adjusted HR: 3.032 (1.817, 5.060); P<0.001) expression and M stage (adjusted HR=4.075 (1.179-14.091) for M1 vs. M0, P=0.026) served as prognostic indicators of unfavorable overall survival in an independent manner in patients with HCC. The C-index for the nomogram was 0.732 (95% CI: 0.698, 0.766) and the AUC of MED8 was 0.817 (95% CI: 0.778, 0.857). Functional analysis showed that the cell cycle checkpoints, p53 dependent G1-DNA damage response, mitotic G1-G1-S phases, and mitotic G2-G2-M phases, were significantly enriched in DEGs associated with MED8 expression. Th2 cells were positively correlated with MED8 expression.

Conclusions: MED8 predicts poor prognosis in HCC, possibly through modulating the cell cycle and Th2 cells.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991165PMC
March 2022

Discovery, optimization and evaluation of 1-(indolin-1-yl)ethan-1-ones as novel selective TRIM24/BRPF1 bromodomain inhibitors.

Eur J Med Chem 2022 Jun 28;236:114311. Epub 2022 Mar 28.

Guangdong Provincial Key Laboratory of Biocomputing, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, 510530, China; State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; China-New Zealand Joint Laboratory on Biomedicine and Health, Guangzhou, 510530, China. Electronic address:

TRIM24 (tripartite motif-containing protein 24) and BRPF1 (bromodomain and PHD finger containing protein 1) are epigenetics "readers" and potential therapeutic targets for cancer and other diseases. Here we describe the structure-guided design of 1-(indolin-1-yl)ethan-1-ones as novel TRIM24/BRPF1 bromodomain inhibitors. The representative compound 20l (Y08624) is a new TRIM24/BRPF1 dual inhibitor, with IC values of 0.98 and 1.16 μM, respectively. Cellular activity of 20l was validated by viability assay in prostate cancer (PC) cell lines. In PC xenograft models, 20l suppressed tumor growth (50 mg/kg/day, TGI = 53%) without exhibiting noticeable toxicity. Compound 20l represents a versatile starting point for the development of more potent TRIM24/BRPF1 inhibitors.
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http://dx.doi.org/10.1016/j.ejmech.2022.114311DOI Listing
June 2022

Can transrectal ultrasonography distinguish anorectal malignant melanoma from low rectal adenocarcinoma? A retrospective paired study for ten years.

BMC Gastroenterol 2022 Apr 5;22(1):165. Epub 2022 Apr 5.

Department of Diagnostic Ultrasound, West China Hospital, Sichuan University, No. 37 Guoxue Road, Wuhou District, Chengdu, 610041, Sichuan Province, China.

Background: Anorectal malignant melanoma (ARMM) and low rectal adenocarcinoma (LRAC) have some similarities in clinical behaviors, histopathological characteristics and ultrasonographic findings, diagnostic errors are common. By comparing the transrectally ultrasonographic features between the two tumors, we propose to provide more possibilities in differentiating them.

Methods: The data of 9 ARMMs and 27 age- and gender-matched LRACs (the lower margin below the peritoneal reflection) in West China Hospital Sichuan University between April 2008 and July 2019 were retrospectively reviewed. The ultrasonic features between the two groups were compared.

Results: Transrectal ultrasonography (TRUS) showed that the length of ARMM was shorter than that of LRAC (28.22 ± 12.29 mm vs. 40.22 ± 15.16 mm), and ARMM had a lower position than that of LRAC (the distance to anal verge was 50.78 ± 11.70 vs. 63.81 ± 18.73 mm). Unlike LRAC, the majority of ARMM in our study was confined to the intestinal mucosa/submucosa (66.67/25.93%) (P < 0.05).

Conclusions: Based on the data of our study, several ultrasonographic findings (length, invasion depth, and position) of ARMM were significantly different from LRAC. Accordingly, more attention should be paid to masses at anorectal junction with lower position, shorter length, and shallower infiltration depth. Instead of the most common tumor, LRAC, ARMM should be taken into account to avoid a misdiagnosis, which will result in a poorer prognosis.
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http://dx.doi.org/10.1186/s12876-022-02237-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985288PMC
April 2022

Correlation between different equations to calculate free testosterone for improved detection of hypogonadism in people living with HIV.

Int J STD AIDS 2022 May 4;33(6):613-617. Epub 2022 Apr 4.

4615Department of Infectious Diseases, Imperial College London, London, UK.

The diagnosis of hypogonadism in people living with HIV (PLWH) remains challenging by the lack of a standardised diagnostic algorithm. Since sexual hormone-binding globulin levels are commonly increased in PLWH, guidelines recommend assessing free testosterone (FT) along with total testosterone levels. We compared different online equations available to estimate FT levels and found a good correlation amongst all algorithms. Estimating FT levels increased diagnostic accuracy of hypogonadism and therefore should be encouraged in clinical practice in PLWH with clinical symptoms of hypogonadism, even when total testosterone levels are normal.
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http://dx.doi.org/10.1177/09564624221083760DOI Listing
May 2022

Evolving Antithrombotic Treatment Patterns for Patients With Nonvalvular Atrial Fibrillation and Acute Coronary Syndrome or Underwent Percutaneous Coronary Intervention in China: A Cross-Sectional Study.

Front Cardiovasc Med 2022 18;9:846803. Epub 2022 Mar 18.

The Emergency and Intensive Care Center, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: Antithrombotic therapy in patients with nonvalvular atrial fibrillation (NVAF) concomitant with the acute coronary syndrome (ACS) or underwent percutaneous coronary intervention (PCI) is challenging and has evolved in recent years. However, real-world data on this issue about antithrombotic regimens at discharge and its evolving trend were relatively scarce, especially in China.

Methods: A total of 2,182 patients with NVAF and ACS/PCI were enrolled from 2017 to 2019. A total of 1,979 patients were finally analyzed and divided in three sequential cohorts: cohort 1 (2017), = 674; cohort 2 (2018), = 793; and cohort 3 (2019), = 512. Baseline characteristics and antithrombotic therapy at discharge were analyzed by cohort.

Results: In our cross-sectional study, the majority of patients (59.6%) received dual antiplatelet therapy (DAPT). Over the 3 years, DAPT prescription reduced from nearly 70% to <50% ( trend < 0.001), while triple therapy (TT)/double therapy (DT) increased from 27.2 to 50.0% ( trend < 0.001). This trend was also seen in different subgroups stratified by CHA2DS2-VASc score, HAS-BLED score, coronary artery disease type, or management type, and was validated after multivariate adjustment. Persistent atrial fibrillation and history of congestive heart failure, hypertension, diabetes mellitus, and stroke/transient ischemic attack/systemic embolism were the independent predictors of TT/DT use, while ACS, PCI, or advanced chronic kidney disease was related with more DAPT prescription.

Conclusion: There is a shift of antithrombotic regime at discharge for patients with NVAF with recent ACS/PCI with reducing DAPT prescription and increasing TT/DT prescription. While the appropriate antithrombotic regimen for patients with NVAF having ACS/PCI is still underused in China.
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http://dx.doi.org/10.3389/fcvm.2022.846803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8971844PMC
March 2022

Impact of renin-angiotensin-aldosterone-system inhibitor drugs on mortality in patients with atrial fibrillation and hypertension.

BMC Cardiovasc Disord 2022 04 1;22(1):141. Epub 2022 Apr 1.

Emergency Center, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167 Beilishi Road, Xicheng District, Beijing, People's Republic of China.

Background: Renin-angiotensin-aldosterone-system inhibitors markedly play an active role in the primary prevention of atrial fibrillation (AF), but the impact of angiotensin-converting enzyme inhibitors (ACEIs) or angiotensin receptor blockers (ARBs) on the mortality of patients with AF remains unclear. This study aimed to examine the relationship between treatment with ACEIs or ARBs and mortality in emergency department (ED) patients with AF and hypertension.

Methods: This multicenter study enrolled 2016 ED patients from September 2008 to April 2011; 1110 patients with AF and hypertension were analyzed. Patients were grouped according to whether they were treated with ACEI/ARB or not and completed a 1-year follow-up to evaluate outcomes including all-cause death, cardiovascular death, stroke, and major adverse events (MAEs).

Results: Among the 1110 patients with AF and hypertension, 574 (51.7%) received ACEI/ARB treatment. During the 1-year follow-up, 169 all-cause deaths (15.2%) and 100 cardiovascular deaths (9.0%) occurred, while 98 strokes (8.8%) and 255 MAEs (23.0%) occurred. According to the multivariate Cox regression analysis, ACEI/ARB therapy was significantly associated with a reduced risk of all-cause death (HR, 0.605; 95% CI 0.431-0.849; P = 0.004). Moreover, ACEI/ARB therapy was independently associated with a reduced risk of cardiovascular death (HR 0.585; 95% CI 0.372-0.921; P = 0.020) and MAEs (HR 0.651, 95% CI 0.496-0.855, P = 0.002) after adjusting for other risk factors.

Conclusions: Our results revealed that ACEI/ARB therapy was independently associated with a reduced risk of all-cause death, cardiovascular death, and MAEs in ED patients with AF and hypertension. These results provide evidence for a tertiary preventive treatment for patients with AF and hypertension.
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http://dx.doi.org/10.1186/s12872-022-02580-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973677PMC
April 2022

The complete chloroplast genome of halophyte (Pall.) Moq., a dominant species of desert grassland.

Mitochondrial DNA B Resour 2022 25;7(3):544-545. Epub 2022 Mar 25.

School of Environmental Engineering, Xuzhou University of Technology, Xuzhou, China.

(Pall.) Moq. is a dominant halophyte species of the desert ecosystem mainly distributed in Southeast Europe and Northwest Asia, and used as a major forage grass. Here, we report its complete chloroplast genome, assembled from the whole-genome resequencing data. The circular genome of is 153,773 bp in length, including a typical quadripartite structure consisting of a pair of inverted repeats (IRs; 24,991 bp) separated by large single-copy (LSC; 84,781 bp) and small single-copy (SSC; 19,010 bp) regions. The total GC content is 36.3%, and a total of 129 genes are annotated, including 84 protein-coding genes, 37 tRNAs, and eight rRNAs. The phylogenetic analysis has shown that is positioned as a sister taxon to the two species, all belonging to the same tribe, Salicornieae.
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http://dx.doi.org/10.1080/23802359.2022.2054379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959530PMC
March 2022

Predictive value of the stress hyperglycemia ratio in patients with acute ST-segment elevation myocardial infarction: insights from a multi-center observational study.

Cardiovasc Diabetol 2022 Mar 29;21(1):48. Epub 2022 Mar 29.

Emergency Center, National Center for Cardiovascular Disease, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167 Beilishi Road, Xicheng District, Beijing, People's Republic of China.

Background: Stress hyperglycemia is a strong predictor of adverse outcomes in patients with acute myocardial infarction (AMI). Recently, the stress hyperglycemia ratio (SHR) has been designed as an index to identify acute hyperglycemia with true risk; however, data regarding the impact of SHR on the prognosis of ST-segment elevation myocardial infarction (STEMI) remains limited. This study aimed to evaluate the predictive value of the SHR in patients with acute STEMI and to assess whether it can improve the predictive efficiency of the Thrombolysis in Myocardial Infarction (TIMI) risk score.

Methods: This study included 7476 consecutive patients diagnosed with acute STEMI across 274 emergency centers. After excluding 2052 patients due to incomplete data, 5417 patients were included in the final analysis. Patients were divided into three groups according to SHR tertiles (SHR1, SHR2, and SHR3) and were further categorized based on diabetes status. All patients were followed up for major cardiovascular adverse events (MACEs) and all-cause mortality.

Results: After 30 days of follow-up, 1547 MACEs (28.6%) and 789 all-cause deaths (14.6%) occurred. The incidence of MACEs was highest among patients in the SHR3 group with diabetes mellitus (DM) (42.6%). Kaplan-Meier curves demonstrated that patients with SHR3 and DM also had the highest risk for MACEs when compared with other groups (p < 0.001). Moreover, C-statistics improved significantly when SHR3 was added into the original model: the ΔC-statistics (95% confidence interval) were 0.008 (0.000-0.013) in the total population, 0.010 (0.003-0.017) in the DM group, and 0.007 (0.002-0.013) in the non-DM group (all p < 0.05). In the receiver operating characteristic analysis, the area under the curve (AUC) for the original TIMI risk score for all-cause death was 0.760. When an SHR3 value of 1 point was used to replace the history of DM, hypertension, or angina in the original TIMI risk score, the Delong test revealed significant improvements in the AUC value (∆AUC of 0.009, p < 0.05), especially in the DM group (∆AUC of 0.010, p < 0.05).

Conclusion: The current results suggest that SHR is independently related to the risks of MACEs and mortality in patients with STEMI. Furthermore, SHR may aid in improving the predictive efficiency of the TIMI risk score in patients with STEMI, especially those with DM.
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http://dx.doi.org/10.1186/s12933-022-01479-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962934PMC
March 2022

Plasma Big Endothelin-1 Levels and Long-Term Outcomes in Patients With Atrial Fibrillation and Acute Coronary Syndrome or Undergoing Percutaneous Coronary Intervention.

Front Cardiovasc Med 2022 3;9:756082. Epub 2022 Mar 3.

Emergency and Critical Care Center, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: This study aimed to evaluate the association between plasma big ET-1 levels and long-term outcomes in patients with atrial fibrillation (AF) and acute coronary syndrome (ACS) or undergoing percutaneous coronary intervention (PCI).

Methods: A total of 930 patients were enrolled and followed up for a median duration of 2.3 years. According to the optimal cutoff of big ET-1 for predicting all-cause death, these patients were divided into two groups. The primary endpoints were all-cause death and net adverse clinical events (NACE). The secondary endpoints included cardiovascular death, major adverse cardiovascular events (MACE), BARC class ≥ 3 bleeding, and BARC class ≥ 2 bleeding. Cox regressions were performed to evaluate the association between big ET-1 and outcomes.

Results: Based on the optimal cutoff of 0.54 pmol/l, 309 patients (33.2%) had high big ET-1 levels at baseline. Compared to the low big ET-1 group, patients in the high big ET-1 group tended to have more comorbidities, impaired cardiac function, elevated inflammatory levels, and worse prognosis. Univariable and multivariable Cox regressions indicated that big ET-1 ≥ 0.54 pmol/l was associated with increased incidences of all-cause death [HR (95%CI):1.73 (1.10-2.71), = 0.018], NACE [HR (95%CI):1.63 (1.23-2.16), = 0.001], cardiovascular death [HR (95%CI):1.72 (1.01-2.92), = 0.046], MACE [HR (95%CI):1.60 (1.19-2.16), = 0.002], BARC class ≥ 3 [HR (95%CI):2.21 (1.16-4.22), = 0.016], and BARC class ≥ 2 bleeding [HR (95%CI):1.91 (1.36-2.70), < 0.001]. Subgroup analysis indicated consistent relationships between the big ET-1 ≥ 0.54 pmol/l and the primary endpoints.

Conclusion: Elevated plasma big ET-1 levels were independently associated with increased risk of all-cause death, NACE, cardiovascular death, MACE, BARC class ≥ 3 bleeding, and BARC class ≥ 2 bleeding in patients with AF and ACS or undergoing PCI.
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http://dx.doi.org/10.3389/fcvm.2022.756082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927675PMC
March 2022

Spatiotemporal dynamics of brain function during the natural course in a dental pulp injury model.

Eur J Nucl Med Mol Imaging 2022 Mar 19. Epub 2022 Mar 19.

Department of Nuclear Medicine and PET Center, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China.

Purpose: Toothache, a common disorder afflicting most people, shows distinct features at different clinical stages. This study aimed to depict metabolic changes in brain and investigate the potential mechanism involved in the aberrant affective behaviors during the natural process of toothache.

Methods: We investigated the spatiotemporal patterns of brain function during the natural course of toothache in a rat model of dental pulp injury (DPI) by using positron emission tomography (PET).

Results: Glucose metabolism peaked on the 3rd day and gradually decreased in several brain regions after DPI, which was in line with the behavioral and histological results. PET imaging showed that visual pathway was involved in the regulation of toothache. Meanwhile, the process of emotional regulation underlying toothache was mediated by N-methyl-D-aspartic receptor subunit 2B (NR2B) in the caudal anterior cingulate cortex (cACC).

Conclusion: Our results revealed the spatiotemporal neurofunctional patterns during toothache process and preliminarily elucidated the role of NR2B in cACC in the regulation of toothache-related affective behaviors.
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http://dx.doi.org/10.1007/s00259-022-05764-2DOI Listing
March 2022

Bmi1 signaling maintains the plasticity of airway epithelial progenitors in response to persistent silica exposures.

Toxicology 2022 03 12;470:153152. Epub 2022 Mar 12.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China; Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA. Electronic address:

Silicosis is caused by the continuous inhalation of environmental silica dust. The repetitive exposure of silica induces airway epithelial cell injury, leads proliferative exhaustion of epithelial stem cells, ultimately results in the lung remodeling and the development of silicosis. The B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi1) is a pivotal transcription factor in stem cell self-renewal and proliferation of many tissues including the lung, but its role in the airway basal cell proliferation and differentiation during the pathogenesis of silicosis in lung has yet been investigated. In this study, the function of Bmi1 in airway basal cell proliferation and differentiation in response to silica challenge was investigated in lungs of silicosis mice and primary human bronchial epithelia cells (HBECs) exposed to silica dioxide (SiO). Results showed a decreased expression of Bmi1 protein, epithelial basal cell markers Krt14 and Krt5, club cell marker Clara cell secretory protein, and ciliated cell marker acetyl-α-tubulin in silicosis lungs, compared to healthy mice. In consistence, a persistent exposure of SiO reduced the capacity of cell proliferation and differentiation in HBECs, as ascertained by the reduction of differentiated epithelial cell markers and BMI1 expression, while an increased P21-positive senescent cell fraction. Moreover, an overexpression of BMI1 in HBECs reduced the SiO-senescent cells, enhanced the potency of cell proliferation and differentiation, and increased capacity of airway epithelial regeneration in response to the persistent exposure of SiO. These data suggest that Bmi1 is a key transcription factor engaging in maintaining the self-renewal, proliferation and differentiation of epithelial stem cells in lung during the development of silicosis disease.
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http://dx.doi.org/10.1016/j.tox.2022.153152DOI Listing
March 2022

Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.

J Neuroinflammation 2022 Mar 14;19(1):67. Epub 2022 Mar 14.

Department of Neurosurgery, Xijing Hospital, Air Force Military Medical University, No. 127, Changle West Road, Xincheng District, Xi'an, 710032, Shaanxi, China.

Background: Inflammation induced by intracerebral hemorrhage (ICH) is one of the main causes of the high mortality and poor prognosis of patients with ICH. A1 astrocytes are closely associated with neuroinflammation and neurotoxicity, whereas A2 astrocytes are neuroprotective. Homer scaffolding protein 1 (Homer1) plays a protective role in ischemic encephalopathy and neurodegenerative diseases. However, the role of Homer1 in ICH-induced inflammation and the effect of Homer1 on the phenotypic conversion of astrocytes remain unknown.

Methods: Femoral artery autologous blood from C57BL/6 mice was used to create an ICH model. We use the A1 phenotype marker C3 and A2 phenotype marker S100A10 to detect astrocyte conversion after ICH. Homer1 overexpression/knock-down mice were constructed by adeno-associated virus (AAV) infection to explore the role of Homer1 and its mechanism of action after ICH. Finally, Homer1 protein and selumetinib were injected into in situ hemorrhage sites in the brains of Homer1/Nestin-Cre mice to study the efficacy of Homer1 in the treatment of ICH by using a mouse cytokine array to explore the potential mechanism.

Results: The expression of Homer1 peaked on the third day after ICH and colocalized with astrocytes. Homer1 promotes A1 phenotypic conversion in astrocytes in vivo and in vitro. Overexpression of Homer1 inhibits the activation of MAPK signaling, whereas Homer1 knock-down increases the expression of pathway-related proteins. The Homer1 protein and selumetinib, a non-ATP competitive MEK1/2 inhibitor, improved the outcome in ICH in Homer1/Nestin-Cre mice. The efficacy of Homer1 in the treatment of ICH is associated with reduced expression of the inflammatory factor TNFSF10 and increased expression of the anti-inflammatory factors activin A, persephin, and TWEAK.

Conclusions: Homer1 plays an important role in inhibiting inflammation after ICH by suppressing the A1 phenotype conversion in astrocytes. In situ injection of Homer1 protein may be a novel and effective method for the treatment of inflammation after ICH.
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http://dx.doi.org/10.1186/s12974-022-02428-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922810PMC
March 2022

Downregulation of ZC3H13 by miR-362-3p/miR-425-5p is associated with a poor prognosis and adverse outcomes in hepatocellular carcinoma.

Aging (Albany NY) 2022 03 12;14(5):2304-2319. Epub 2022 Mar 12.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, People's Republic of China.

Hepatocellular carcinoma (HCC) is notorious for its poor prognosis. Previous studies identified several N6-methyladenosine (m6A)-related genes that play key roles in the initiation and progression of HCC patients. In particular, the N6-methyladenosine RNA methylation regulator ZC3H13 could be a candidate as a novel biomarker and therapeutic target for hepatocellular carcinoma. In HCC, low expression of ZC3H13 was reported, but the molecular reason is unclear. In this study, we performed pan cancer analysis for ZC3H13 expression and prognosis using The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data and found that ZC3H13 might be a potential tumor suppressor gene in HCC. Subsequently, miRNAs contributing to ZC3H13 downregulation were identified by a series of analyses, including expression analysis, correlation analysis, and survival analysis. Finally, the miR-362-3p/miR-425-5p-ZC3H13 axis was identified as the most likely upstream miRNA-related pathway of ZC3H13 in HCC. Additionally, miR-362-3p/miR-425-5p mimic and inhibitor results were detected by quantitative real-time PCR (qPCR) analysis and western blotting. We identified an upstream regulatory mechanism of ZC3H13 in HCC, namely, the miR-362-3p/miR-425-5p-ZC3H13 axis. Moreover, the ZC3H13 level was significantly positively associated with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression. Collectively, our findings elucidated that ncRNA-mediated downregulation of ZC3H13 was correlated with a poor prognosis and tumor immune infiltration in HCC. In conclusion, this study demonstrates that ZC3H13 is a direct target of miR-362-3p/miR-425-5p in liver hepatocellular carcinoma (LIHC) that regulates immune modulation in the microenvironment of LIHC.
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http://dx.doi.org/10.18632/aging.203939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954979PMC
March 2022

Cooperative P-Wave Velocity Measurement with Full Waveform Moment Tensor Inversion in Transversely Anisotropic Media.

Sensors (Basel) 2022 Mar 1;22(5). Epub 2022 Mar 1.

School of Resources and Safety Engineering, Central South University, Changsha 410083, China.

Precise stochastic approaches to quantitatively calculate the source uncertainties offers the opportunity to eliminate the influence of anisotropy on moment tensor inversion. The effects of ignoring anisotropy were tested by using homogeneous Green's functions. Results indicate the influence of anisotropy and noise on fault plane rotation is very small for a pure shear source whether it is restricted to double couple solution or full moment tensor solution. Green's functions with different prior rough anisotropy information were tested, indicating that the complex source is more sensitive to velocity models than the pure shear source and the fault plane rotation caused by full moment tensor solution is larger than the pure double couple solution. Collaborative P-wave velocity inversion with active measurements and passive acoustic emission data using the fast-marching method were conducted, and new Green's functions established based on the tomography results. The resolved fault plane solution rotated only 3.5° when using the new Green's functions, but the presence of spurious isotropic and compensated linear vector dipole components was not completely eliminated. It is concluded that the cooperative inversion is capable of greatly improving the accuracy of the fault plane solutions and reducing the spurious components in the full moment tensor solution.
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http://dx.doi.org/10.3390/s22051935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914914PMC
March 2022
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