Publications by authors named "Shuang Wang"

1,597 Publications

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Ferritin-based targeted delivery of arsenic to diverse leukaemia types confers strong anti-leukaemia therapeutic effects.

Nat Nanotechnol 2021 Oct 25. Epub 2021 Oct 25.

Beijing National Laboratory for Molecular Engineering, College of Chemistry and Molecular Engineering and College of Engineering, and BIC-ESAT, Peking University, Beijing, P. R. China.

Trivalent arsenic (As) is an effective agent for treating patients with acute promyelocytic leukaemia, but its ionic nature leads to several major limitations like low effective concentrations in leukaemia cells and substantial off-target cytotoxicity, which limits its general application to other types of leukaemia. Here, building from our clinical discovery that cancerous cells from patients with different leukaemia forms featured stable and strong expression of CD71, we designed a ferritin-based As nanomedicine, [email protected], that bound to leukaemia cells with very high affinity, and efficiently delivered cytotoxic As into a large diversity of leukaemia cell lines and patient cells. Moreover, [email protected] exerted strong anti-leukaemia effects in diverse cell-line-derived xenograft models, as well as in a patient-derived xenograft model, in which it consistently outperformed the gold standard, showing its potential as a precision treatment for a variety of leukaemias.
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http://dx.doi.org/10.1038/s41565-021-00980-7DOI Listing
October 2021

ΔCT Value of Amplified Refractory Mutation System Predicts Efficacy of EGFR-TKIs in Advanced Non-Small-Cell Lung Cancer: A Multi-Center Retrospective Study.

Front Mol Biosci 2021 8;8:684661. Epub 2021 Oct 8.

Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

This multi-center retrospective study determines whether the ΔCT value of the Amplified Refractory Mutation System (ARMS) predicts the efficacy of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) in EGFR-mutant advanced non-small-cell lung cancer (NSCLC). Patients who harbored an exon 19 deletion (19Del) or L858R mutation detected by the ARMS and previously received treatment of EGFR-TKIs as a monotherapy were enrolled. A total of 108 NSCLC patients in four hospitals were enrolled. We divided the patients into a high ΔCT group (Group H) and a low ΔCT group (Group L) by the Martingale residuals analysis and log-rank test. The primary outcome was progression-free survival (PFS). Univariate analysis and multivariable regression were applied to compare the PFS between the groups. The Martingale residuals analysis and log-rank test were applied to find the cutoff ΔCT value (0.8). In the 108 patients we enrolled, 59 were in group L and 49 were in group H. Patients' demographics and clinical characteristics, including age, sex, smoking history, pathology, mutation sites, TNM stage, and line of TKIs therapy, were not significantly different between group L and group H. The median PFS was 11.1 months in group L and 6.9 months in group H, and the difference showed statistical significance ( < 0.001). Moreover, the objective response rates (ORRs) in group L was significantly higher than in group H (61.0 vs 34.7%, = 0.002). The median OS was 25.0 months in group L and 20.0 months in group H ( = 0.046). The ΔCT value of ARMS could be an efficacy predictor for EGFR-TKI treatment in advanced EGFR-mutant NSCLC.
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http://dx.doi.org/10.3389/fmolb.2021.684661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531541PMC
October 2021

Unveiling osteosarcoma responses to DAPT combined with cisplatin by using confocal Raman microscopy.

Biomed Opt Express 2021 Sep 11;12(9):5514-5528. Epub 2021 Aug 11.

Institute of Photonics and Photon-Technology, Northwest University, Xi'an, Shaanxi 710069, China.

The aim of this study was to clarify the dose- and time-dependent effect of the γ-secretase inhibitor (DAPT) combined with cisplatin on osteosarcoma (OS) cells, evaluated by confocal Raman microspectral imaging (CRMI) technology. The intracellular composition significantly changed after combined drug action compared with the sole cisplatin treatment, proving the synergistic effect of DAPT combined with cisplatin on OS cells. The principal component analysis-linear discriminant analysis revealed the main compositional variations by distinguishing spectral characteristics. K-means cluster and univariate imaging were used to visualize the changes in subcellular morphology and biochemical distribution. The results showed that the increase of the DAPT dose and cisplatin treatment time in the combination treatment induced the division of the nucleus in OS cells, and other organelles also showed significant physiological changes compared with the effect of sole cisplatin treatment. After understanding the cellular response to the combined drug treatment at a molecular level, the achieved results provide an experimental fact for developing suitable individualized tumor treatment protocols.
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http://dx.doi.org/10.1364/BOE.432933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515968PMC
September 2021

Networking retinomorphic sensor with memristive crossbar for brain-inspired visual perception.

Natl Sci Rev 2021 Feb 25;8(2):nwaa172. Epub 2020 Jul 25.

National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

Compared to human vision, conventional machine vision composed of an image sensor and processor suffers from high latency and large power consumption due to physically separated image sensing and processing. A neuromorphic vision system with brain-inspired visual perception provides a promising solution to the problem. Here we propose and demonstrate a prototype neuromorphic vision system by networking a retinomorphic sensor with a memristive crossbar. We fabricate the retinomorphic sensor by using WSe/h-BN/AlO van der Waals heterostructures with gate-tunable photoresponses, to closely mimic the human retinal capabilities in simultaneously sensing and processing images. We then network the sensor with a large-scale Pt/Ta/HfO/Ta one-transistor-one-resistor (1T1R) memristive crossbar, which plays a similar role to the visual cortex in the human brain. The realized neuromorphic vision system allows for fast letter recognition and object tracking, indicating the capabilities of image sensing, processing and recognition in the full analog regime. Our work suggests that such a neuromorphic vision system may open up unprecedented opportunities in future visual perception applications.
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http://dx.doi.org/10.1093/nsr/nwaa172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288371PMC
February 2021

Can resting-state fMRI help localize the seizure onset zone in focal epilepsies?

Authors:
Hong Li Shuang Wang

Clin Neurophysiol 2021 Oct 8. Epub 2021 Oct 8.

Department of Neurology, Epilepsy Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, China. Electronic address:

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http://dx.doi.org/10.1016/j.clinph.2021.09.009DOI Listing
October 2021

Response to antiseizure medications in epileptic patients with malformation of cortical development.

Ther Adv Neurol Disord 2021 12;14:17562864211050027. Epub 2021 Oct 12.

Department of Neurology, Epilepsy Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.

Background: Malformation of cortical development (MCD) is one of the most common causes of pharmacoresistant epilepsy. Improving the knowledge of antiseizure medications (ASMs) treatment response in epileptic patients with MCD is crucial for optimal treatment options, either pharmacological therapy or non-pharmacological intervention.

Aim: To investigate the patterns of medical treatment outcome and the predictors for seizure freedom (SF) with ASM regimens in epilepsy caused by MCD.

Methods: The epileptic patients with MCD were consecutively enrolled from March 2013 to June 2019. SF was defined as no seizures for at least 12 months or three times the longest pretreatment inter-seizure interval, whichever was longer. Outcomes were classified into three patterns: pattern A: patients achieved SF at one point and remained so throughout follow-up; pattern B: patients' seizures fluctuated between periods of SF and relapse; pattern C: SF never attained. The terminal SF was defined if the patients remained SF at the last follow-up visit.

Results: A total of 164 epileptic patients with MCD were included. Pattern A was observed in 22, pattern B in 42, and pattern C in 100 patients. SF was ever achieved in 64 (pattern A and B) patients. Twenty-nine patients had terminal SF after a median follow-up time of 4.3 years. With continuing ASM treatment, seizure relapse risk was very low after a 5-year seizure-free period. The pretreatment seizure frequency was the only independent predictor for pattern A and seizure relapse. Sodium channel blockers monotherapy (33.8%) was more effective than levetiracetam (4.5%) in rendering SF in the initial ASM regimen.

Conclusion: Medical treatment can be successful in a minority of epileptic patients with MCD, and pretreatment seizure frequency helps to predict the treatment outcome. An unequal efficacy of ASMs in epilepsy caused by MCD suggests etiological evaluation is vital in the management of focal epilepsy.
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http://dx.doi.org/10.1177/17562864211050027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521419PMC
October 2021

Keratin 80 regulated by miR-206/ETS1 promotes tumor progression via the MEK/ERK pathway in ovarian cancer.

J Cancer 2021 24;12(22):6835-6850. Epub 2021 Sep 24.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Liaoning, China.

Keratin 80 (KRT80) is a type II epithelial keratin protein that plays an important role in cell differentiation and tumor progression. However, its role and mechanisms in ovarian cancer remain unclear. The effect of KRT80 on the survival and prognosis of patients with ovarian cancer was determined using immunohistochemistry. Cell lines overexpressing KRT80 and with KRT80 knockdown were established to study its effect on the malignant behavior of ovarian cancer cells. Western blotting was used to detect changes in related molecules, and in the MEK/ERK signal transduction pathway. ChIP assay was used to confirm that ETS1 regulates KRT80 at the transcriptional level. A double luciferase assay was used to confirm the target of miR-206. The expression levels of KRT80 were high in ovarian cancer tissue, and were related to survival and prognosis. KRT80 expression is an independent prognostic factor in patients with ovarian cancer. KRT80 overexpression promotes the proliferation of ovarian cancer cells, the transition from G1 phase to S phase, invasion, and migration. KRT80 overexpression increased the expression of BCL2/BAX, CyclinD1, MMP2, MMP9, and N-cadherin, decreased the expression of E-cadherin, and increased the phosphorylation of MEK and ERK. ETS1 binds to the upstream promoter sequence of KRT80 and regulates KRT80 expression at the transcriptional level. ETS1 is a direct target of miR-206 in ovarian cancer cells. KRT80 regulated by miR-206/ETS1 promotes tumor progression via the MEK/ERK pathway in ovarian cancer, and KRT80 may have applications as a screening biomarker and potential therapeutic target for ovarian cancer.
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http://dx.doi.org/10.7150/jca.64031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517993PMC
September 2021

Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment.

Sci Transl Med 2021 Oct 13;13(615):eabb6981. Epub 2021 Oct 13.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.

[Figure: see text].
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http://dx.doi.org/10.1126/scitranslmed.abb6981DOI Listing
October 2021

Recent advances of thermochemical conversion processes for biorefinery.

Bioresour Technol 2021 Oct 9;343:126109. Epub 2021 Oct 9.

School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, Dongdaemun-gu, Seoul, Republic of Korea. Electronic address:

Lignocellulosic biomass is one of the most promising renewable resources and can replace fossil fuels via various biorefinery processes. Through this study, we addressed and analyzed recent advances in the thermochemical conversion of various lignocellulosic biomasses. We summarized the operation conditions and results related to each thermochemical conversion processes such as pyrolysis (torrefaction), hydrothermal treatment, gasification and combustion. This review indicates that using thermochemical conversion processes in biorefineries is techno-economically feasible, easy, and effective compared with biological processes. The challenges experienced in thermochemical conversion processes are also presented in this study for better understanding the future of thermochemical conversion processes for biorefinery. With the aid of artificial intelligence and machine learning, we can reduce time-consumption and experimental work for bio-oil production and syngas production processes.
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http://dx.doi.org/10.1016/j.biortech.2021.126109DOI Listing
October 2021

Short- and medium-chain chlorinated paraffins in soil from an urban area of northern China: Levels, distribution, and homolog patterns.

Sci Total Environ 2021 Oct 8;807(Pt 2):150833. Epub 2021 Oct 8.

Jianghan University, Wuhan 430056, China. Electronic address:

Short-chain chlorinated paraffins (SCCPs) are persistent organic pollutants that are present in relatively high concentrations in various environmental media in China. Many studies have focused on chlorinated paraffins in soil from agricultural land and contaminated areas. There are limited data on the levels of chlorinated paraffins in soil from urban areas. In this study, to investigate the levels, distribution, and homolog patterns of chlorinated paraffins (CPs) in soil from a typical urban area, 130 soil samples were collected and combined to form 26 pooled samples. The samples were analyzed for 50 CP congener groups (CCl). The concentration ranges for SCCPs, medium-chain CPs (MCCP), and chlorinated nonane paraffin (C-CP) were 19-1456 ng/g (average: 234 ng/g), <10-385 ng/g (average: 54 ng/g), and 1-39 ng/g (average: 11 ng/g), respectively. The CP concentrations were not significantly correlated with the total organic carbon content (P > 0.05). Compared with other areas worldwide, the SCCP and C-CP concentrations in soil in this area were at the medium level, and the concentrations of MCCPs were at a low level. The CP concentrations were higher in soil samples collected near factories and domestic garbage disposal sites. CCl were the main SCCP homologs and CCl were the main MCCP homologs. Principal component analysis showed that the sources of C-CPs, SCCPs, and MCCPs in the soils were similar. Risk assessment showed that the concentrations of SCCPs and MCCPs in soil in this area did not pose a significant risk to soil organisms or human health.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150833DOI Listing
October 2021

Hemorrhagic pericardial effusion following treatment with infliximab: A case report and literature review.

World J Clin Cases 2021 Sep;9(25):7593-7599

Department of Gastroenterology, The 2 Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China.

Background: Infliximab (IFX) is an anti-tumor necrosis factor alpha (TNF-α) agent that is widely used for the management of a variety of autoimmune and inflammatory diseases, including Crohn's disease (CD). As a result of its increasing administration, new complications have emerged. Hemorrhagic pericardial effusion, secondary to IFX therapy, is a rare but life-threatening complication.

Case Summary: A 27-year-old man was diagnosed with CD (Montreal A2L3B1) 6 years prior. After failing to respond to mesalazine and methylprednisolone, he took the first dose of IFX 300 mg based on his weight (60 kg, dose 5 mg/kg) on December 3, 2018. He responded well to this therapy. However, on January 21, 2019, 1 wk after the third injection, he suddenly developed dyspnea, fever, and worsening weakness and was admitted to our hospital. On admission, computed tomography scan of the chest revealed a large pericardial effusion and a small right-side pleural effusion. An echocardiogram showed a large pericardial effusion and normal left ventricular function. Then successful ultrasound-guided pericardiocentesis was performed and 600 mL hemorrhagic fluid was drained. There was no evidence of infection and the concentrations of TNF-α, IFX, and anti-IFX antibody were 7.09 pg/mL (reference range < 8.1 pg/mL), < 0.4 μg/mL (> 1.0 μg/mL), and 373 ng/mL (< 30 ng/mL), respectively. As the IFX instruction manual for injection does mention pericardial effusion as a rare adverse reaction (≥ 1/10000, < 1/1000), so we discontinued the IFX. Monitoring of the patient's echocardiogram for 2 mo without IFX therapy showed no recurrence of hemorrhagic pericardial effusion. Follow-up visits and examinations every 3 to 6 mo until April 2021 showed no recurrence of CD or pericardial effusion.

Conclusion: This is a case of hemorrhagic pericardial effusion following treatment with IFX. It is a rare but life-threatening complication of IFX. Early recognition helps prevent the occurrence of hemorrhagic pericardial effusion and minimize the impact on the natural evolution of the disease.
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http://dx.doi.org/10.12998/wjcc.v9.i25.7593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464448PMC
September 2021

Heterogeneous-frequency-dual-pulse chain and weak FBG array for quasi-distributed acoustic sensing with improved response bandwidth.

Appl Opt 2021 Sep;60(25):7740-7744

By continuously injecting four groups of heterogeneous frequency dual pulses into the sensing fiber with weak fiber Bragg gratings (WFBGs), a quasi-distributed acoustic sensing method based on frequency-division multiplexing is proposed. Each group of pulses generates interference signals with different carrier frequencies after being reflected by the WFBGs. Through the discrete Fourier transform phase demodulation method, each carrier frequency interference signal is demodulated and then the phase is spliced. The feasibility of this method is theoretically analyzed, and a detection with a bandwidth of 2 kHz is realized on a 70 km sensing fiber with a spatial resolution of 10 m.
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http://dx.doi.org/10.1364/AO.432727DOI Listing
September 2021

Nano-selenium attenuates mitochondrial-associated apoptosis via the PI3K/AKT pathway in nickel-induced hepatotoxicity in vivo and in vitro.

Environ Toxicol 2021 Oct 6. Epub 2021 Oct 6.

Key Laboratory of Materials Physics and Anhui Key Laboratory of Nanomaterials and Nanotechnology, Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.

The aim of this study was to investigate the protective effects of Nano-Se against nickel (Ni)-induced hepatotoxicity and the potential mechanism. Hence, we constructed in vivo and in vitro models of Ni-induced hepatotoxicity. Sprague-Dawley (SD) rats were exposed to nickel sulfate (NiSO , 5.0 mg/kg, i.p.) with or without Nano-Se (0.5, 1, and 2 mg/kg, oral gavage) co-administration for 14 days, and HepG2 cells were exposed to NiSO (1500 μM) with or without Nano-Se (20 μM) for 24 h. Nano-Se obviously prevented Ni-induced hepatotoxicity indicated by ameliorating pathological change and decreasing Ni accumulation in rat livers. Ni induced a significant increase in hepatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH-Px), and malondialdehyde (MDA) level, decreased the glutathione (GSH) content while compared to those in the control group. Nano-Se administration improved the hepatic antioxidant capacity through increase hepatic GSH contents and GSH-Px activity, decrease the activities of SOD, CAT, and MDA level. Nano-Se improved the cell viability, decreased active oxygen (ROS) generation and ameliorated morphological changes of nuclear structures in Ni-treated HepG2 cells. In addition, Nano-Se inhibited the Ni-induced increases of cytochrome c, caspase-9, cleaved caspase-3, increased PI3K and AKT phosphorylation both in vivo and in vitro. Besides, the PI3K inhibitor Y294002 could inhibit the protective effects of Nano-Se on apoptosis. Thus, Nano-Se significantly activates PI3K/AKT signaling to ameliorate apoptosis in Ni-induced hepatotoxicity.
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http://dx.doi.org/10.1002/tox.23381DOI Listing
October 2021

Skin-conformal MXene-doped wearable sensors with self-adhesive, dual-mode sensing, and high sensitivity for human motions and wireless monitoring.

J Mater Chem B 2021 Oct 5. Epub 2021 Oct 5.

College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, P. R. China.

Flexible sensors have attracted extensive attention due to their excellent flexibility, biocompatibility, and information acquisition accuracy. Therefore, it is desired to fabricate a flexible sensor with high toughness and sensitivity based on conductive hydrogels to monitor human movement. In this work, MXene-(TiCT-)WPU/PAM dual-network hydrogels (PPM hydrogels) were successfully prepared. As the first network, waterborne polyurethane (WPU) plays the role of energy dissipation and enhancement. Polyacrylamide (PAM) and WPU polymer chains form interpenetrating networks (IPNs). MXene acts as a conductive material to enhance the conductivity and for nano enhancement. The PPM hydrogels exhibited excellent mechanical characteristics (tensile ratio >600%, tensile strength 639 kPa, 1000 stretching cycles, and self-recovery rate 93.7%). Moreover, based on these hydrogels, we fabricated flexible sensors. These sensors had high sensitivity and sensing durability, and could be assembled into a human body wireless monitoring device, which possesses great potential in facial micro-expression monitoring, all-around human motion detection, and wearable electronic products. In addition, these resulting hydrogels possessed outstanding reversible adhesion to various materials (human skin, wood, PDMS, ) and the maximum adhesion strength can reach 305.1 N m when exposed to a PDMS substrate. Therefore, PPM hydrogels could provide new inspiration for the development of wearable flexible sensors in the domain of human movements and personalized physiological health monitoring.
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http://dx.doi.org/10.1039/d1tb01769aDOI Listing
October 2021

Maternal Complications and Prescription Opioid Exposure During Pregnancy: Using Marginal Structural Models.

Drug Saf 2021 Oct 5. Epub 2021 Oct 5.

Department of Neurology, Stanford University, Palo Alto, CA, USA.

Introduction: Prescription opioids are frequently used for pain management in pregnancy. Studies examining perinatal complications in mothers who received prescription opioids during pregnancy are still limited.

Objectives: The aim of this study was to assess the association of prescription opioid use and maternal pregnancy and obstetric complications.

Methods: This retrospective cohort study with the Rhode Island (RI) Medicaid claims data linked to vital statistics throughout 2008-2015 included pregnant women aged 12-55 years with one or multiple live births. Women were excluded if they had cancer, opioid use disorder, or opioid dispensing prior to but not during pregnancy. Main outcomes included adverse pregnancy and obstetric complications. Marginal Structural Cox Models with time-varying exposure and covariates were applied to control for baseline and time-varying covariates. Analyses were conducted for outcomes that occurred 1 week after opioid exposure (primary) or within the same week as exposure (secondary). Sensitivity studies were conducted to assess the effects of different doses and individual opioids.

Results: Of 9823 eligible mothers, 545 (5.5%) filled one or more prescription opioid during pregnancy. Compared with those unexposed, no significant risk was observed in primary analyses, while in secondary analyses opioid-exposed mothers were associated with an increased risk of cesarean antepartum depression (HR 3.19; 95% CI 1.22-8.33), and cardiac events (HR 9.44; 95% CI 1.19-74.83). In sensitivity analyses, results are more prominent in high dose exposure and are consistent for individual opioids.

Conclusions: Prescription opioid use during pregnancy is associated with an increased risk of maternal complications.
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http://dx.doi.org/10.1007/s40264-021-01115-6DOI Listing
October 2021

Random walks on complex networks with multiple resetting nodes: A renewal approach.

Chaos 2021 Sep;31(9):093135

Key Laboratory of Advanced Electronic Materials and Devices & School of Mathematics and Physics, Anhui Jianzhu University, Hefei 230601, China.

Due to wide applications in diverse fields, random walks subject to stochastic resetting have attracted considerable attention in the last decade. In this paper, we study discrete-time random walks on complex networks with multiple resetting nodes. Using a renewal approach, we derive exact expressions of the occupation probability of the walker in each node and mean first-passage time between arbitrary two nodes. All the results can be expressed in terms of the spectral properties of the transition matrix in the absence of resetting. We demonstrate our results on circular networks, stochastic block models, and Barabási-Albert scale-free networks and find the advantage of the resetting processes to multiple resetting nodes in a global search on such networks. Finally, the distribution of resetting probabilities is optimized via a simulated annealing algorithm, so as to minimize the mean first-passage time averaged over arbitrary two distinct nodes.
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http://dx.doi.org/10.1063/5.0064791DOI Listing
September 2021

A novel biochar electrode for efficient electroreduction of nitrate: Selective and regulation of halogen.

Chemosphere 2021 Sep 28;288(Pt 2):132400. Epub 2021 Sep 28.

Shenyang Monitoring Station of National Municipal Water Supply Quality Monitoring Network, Shenyang, 110301, PR China.

A novel biochar electrode Bio-FeO/CF used for electroreduction of nitrate was prepared by the hydrothermal synthesis method. The results showed that the growth of spherical FeO on the surface of smooth biochar can significantly increase the nitrate reduction rate. Besides, the presence of Cl and Br in the solution could promote the conversion of NH to N, thereby regulating the element nitrogen in the solution. Mechanistic analysis showed that the interconversion of Fe (II) and Fe (III) facilitates the transfer of electrons to nitrate. This study not only provides a biochar electrode material for the efficient removal of nitrate but also simply reveals regulation of halogen in solution, which provides a particular theoretical and data basis for nitrate removal.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132400DOI Listing
September 2021

Effects of Bicarbonate Stress on Serum Ions and Gill Transporters in Alkali and Freshwater Forms of Amur Ide ().

Front Physiol 2021 14;12:676096. Epub 2021 Sep 14.

National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.

The Amur ide () is a fish in the Cyprinidae family. Compared with other Amur ide living in freshwater ecosystems, the Amur ide population in Lake Dali Nor of China is famous for its high tolerance to the alkaline conditions of 54 mM (pH 9.6). Yet, surprisingly, the ionoregulatory mechanism responsible for this remarkable alkaline adaptation remains unclear. Therefore, this study sought to investigate how bicarbonate affects the acid-base balancing and ionoregulatory responses of this animal. Here, using a comparative approach, the alkali form of Amur ide and its ancestral freshwater form living in other freshwater basins were each exposed to 50 mM (pH 9.59 ± 0.09), a level close to the alkalinity of Lake Dali Nor, and their physiological (AE1) adjustment of ions and acid-base regulation were investigated. This study highlighted differences in blood pH and serum ions (e.g., Na, K, Cl, and Ca), Na/K ATPase (NKA) activity and its mRNA level, and mRNA expression of gill transporters (Na/H exchanger member 2 and/or 3, Na/ cotransporter (NBC1), Cl/ exchanger, Na/Cl cotransporter (NCC), Na/K/2Cl (NKCC1), SLC26A5, and SLC26A6) for alkalinity adaptation between the two forms of Amur ide differing in alkalinity tolerance. Specifically, close relationships among the serum Na and mRNA levels of NCC, NKCC1, and NHE, and also NKA and NBC1, in addition to serum Cl and bicarbonate transporters (e.g., SLC26A5 and SLC26A6), characterized the alkali form of Amur ide. We propose that this ecotype can ensure its transepithelial Cl and Na uptake/base secretions are highly functional, by its basolateral NKA with NBC1 and apical ionic transporters, and especially NCC incorporated with other transporters (e.g., SLC26). This suggests an evolved strong ability to maintain an ion osmotic and acid-base balance for more effectively facilitating its adaptability to the high alkaline environment. This study provides new insights into the physiological responses of the alkaline form of the Amur ide fish for adapting to extreme alkaline conditions. This information could be used as a reference to cultivating alkaline-tolerant fish species in abandoned alkaline waters.
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http://dx.doi.org/10.3389/fphys.2021.676096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476968PMC
September 2021

Prevalence and transmission of mobilized colistin resistance (mcr-1) gene positive Escherichia coli in healthy rural residents in Shandong province, China.

Microbiol Res 2021 Sep 25;253:126881. Epub 2021 Sep 25.

Bacterial Infection Disease Control of Institute, Shandong Center for Disease Control and Prevention, Jinan, 250014, China. Electronic address:

This study was conducted to explore the prevalence and transmission of mcr-1 Escherichia coli among healthy rural residents in Shandong, China, and to provide theoretical basis for the prevention and control of spread and treatment of multi-drug resistant Escherichia coli. A total of 218 healthy residents from 3 villages in Guan County, Shandong Province, China were included in this study, and their fecal samples were collected. Colistin-resistant Escherichia coli were selected, and their drug sensitivity and plasmids' transferability were measured. After analysis, some conclusions can be drawn. The colistin-resistant Escherichia coli, most strains of which are MDROs, is common and highly transmissible in healthy residents in rural areas in China. Interventions should be implemented to prevent the spread of colistin-resistant Escherichia coli through health education and tighter regulation of antibiotics.
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http://dx.doi.org/10.1016/j.micres.2021.126881DOI Listing
September 2021

Humidity-Driven Switch in the Transparency of a Nanofiber Film for a Smart Window.

J Phys Chem Lett 2021 Oct 29;12(39):9636-9643. Epub 2021 Sep 29.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.

Traditional smart windows use electrical signals to transform transparency. However, this electric transmission mode greatly limits their uses. Here, we have prepared a transparent PVA--PE/CA composite film, which can realize the reversible transformation of transparency under the stimulation of humidity. The preparation method of the composite film included simple immersion and a thermal curing process, showing high optical transparency (96.61%) and an excellent tensile strain at break of 536.34%. Once exposed to moisture stimulation, the rapid hygroscopic swelling of the composite film led to the increase in the difference in the refractive index between the citric acid filling phase and the nanofibers, which directly led to the sharp decrease in the composite film's transparency. Moreover, the composite film can be arbitrarily attached to the surface of the transparent substrate and designed as some special visualization devices or smart windows, which have a promising future in information encryption and intelligent homes.
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http://dx.doi.org/10.1021/acs.jpclett.1c02772DOI Listing
October 2021

Graphene Oxide/Nanofiber-Based Actuation Films with Moisture and Photothermal Stimulation Response for Remote Intelligent Control Applications.

ACS Appl Mater Interfaces 2021 Oct 29;13(40):48179-48188. Epub 2021 Sep 29.

Key Laboratory of Textile Fiber and Products (Wuhan Textile University), Ministry of Education, Wuhan 430200, China.

The rapid development of intelligent technology and industry has induced higher requirements for multifunctional materials, especially intelligent materials with stimulus-responsive self-actuation behavior. In this study, a [email protected]/GO composite actuation film, with an asymmetric sandwich structure, was prepared by attaching graphene oxide (GO) to the surface of a polyvinyl alcohol ethylene copolymer (PVA-co-PE) nanofiber composite film containing copper nanoparticles (Cu) through layer-on-layer adsorption. This unique structural design endowed the composite film with not only excellent structural stability but also different bending directions (in response to moisture and infrared light). The actuation performance shows that when the adsorption time was 4 h, the maximum bending angle of the [email protected]/GO composite film was up to 90° within 5.99 s. Furthermore, the actuation behavior was stable after 100 cycles of reversible moisture stimulation. Additionally, the maximum actuation strain of the composite film was up to 1.35 MPa during the illumination time of 6.8 s and maintained an excellent stability for 400 s under continuous infrared stimulation of 0.53 W/cm. The rapid and sensitive stimulus response of the [email protected]/GO composite film exhibited self-actuation behavior under the remote control of moisture and infrared light. This, in turn, suggests prospects for wide applications in emerging technologies, such as intelligent switches, artificial muscles, intelligent medical treatment, and flexible robots.
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http://dx.doi.org/10.1021/acsami.1c11117DOI Listing
October 2021

Ga Based Particles, Alloys and Composites: Fabrication and Applications.

Nanomaterials (Basel) 2021 Aug 30;11(9). Epub 2021 Aug 30.

Institute of Marine Biomedicine, Shenzhen Polytechnic, Shenzhen 518055, China.

Liquid metal (LM) materials, including pure gallium (Ga) LM, eutectic alloys and their composites with organic polymers and inorganic nanoparticles, are cutting-edge functional materials owing to their outstanding electrical conductivity, thermal conductivity, extraordinary mechanical compliance, deformability and excellent biocompatibility. The unique properties of LM-based materials at room temperatures can overcome the drawbacks of the conventional electronic devices, particularly high thermal, electrical conductivities and their fluidic property, which would open tremendous opportunities for the fundamental research and practical applications of stretchable and wearable electronic devices. Therefore, research interest has been increasingly devoted to the fabrication methodologies of LM nanoparticles and their functional composites. In this review, we intend to present an overview of the state-of-art protocols for the synthesis of Ga-based materials, to introduce their potential applications in the fields ranging from wearable electronics, energy storage batteries and energy harvesting devices to bio-applications, and to discuss challenges and opportunities in future studies.
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http://dx.doi.org/10.3390/nano11092246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471900PMC
August 2021

SLC25A21 Suppresses Cell Growth in Bladder Cancer an Oxidative Stress-Mediated Mechanism.

Front Oncol 2021 9;11:682710. Epub 2021 Sep 9.

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Bladder cancer (BCa) is a commonly diagnosed malignancy worldwide that has poor survival depending on its intrinsic biologic aggressiveness and a peculiar radio- and chemoresistance features. Gaining a better understanding of tumorigenesis and developing new diagnosis and treatment strategies for BCa is important for improving BCa clinical outcome. SLC25 family member 21 (SLC25A21), a carrier transporting C5-C7 oxodicarboxylates, has been reported to contribute to oxoadipate acidemia. However, the potential role of SLC25A21 in cancer remains absolutely unknown.

Methods: The expression levels of SLC25A21 in BCa and normal tissues were examined by real-time PCR and immunohistochemistry. Gain-of- and loss-of-function experiments were performed to detect the biological functions of SLC25A21 and by CCK-8 assay, plate colony formation assay, cell migration, invasion assay and experimental animal models. The subcellular distribution of substrate mediated by SLC25A21, mitochondrial membrane potential and ROS production were assessed to explore the potential mechanism of SLC25A21 in BCa.

Results: We found that the expression of SLC25A21 was downregulated in BCa tissues compared to normal tissues. A significant positive correlation between decreased SLC25A21 expression and poor prognosis was observed in BCa patients. Overexpression of SLC25A21 significantly inhibited cell proliferation, migration and invasion and induced apoptosis . Moreover, the enhanced SLC25A21 expression significantly suppressed tumor growth in a xenograft mouse model. Furthermore, we revealed that SLC25A21 suppressed BCa growth by inducing the efflux of mitochondrial α-KG to the cytosol, decreasing to against oxidative stress, and activating the ROS-mediated mitochondrion-dependent apoptosis pathway.

Conclusions: Our findings provide the first link between SLC25A21 expression and BCa and demonstrate that SLC25A21 acts as a crucial suppressor in BCa progression, which may help to provide new targets for BCa intervention.
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http://dx.doi.org/10.3389/fonc.2021.682710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458862PMC
September 2021

Ketamine Induces Ferroptosis of Liver Cancer Cells by Targeting lncRNA PVT1/miR-214-3p/GPX4.

Drug Des Devel Ther 2021 18;15:3965-3978. Epub 2021 Sep 18.

Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, 110001, Liaoning, People's Republic of China.

Background: Liver cancer ranks the top four malignant cancer type worldwide, which needs effective and safe treatment. Ferroptosis is a novel form of regulated cell death driven by iron-dependent lipid peroxidation and has been regarded as a promising therapeutic target for cancers. In this work, we aimed to study the effects of anesthetic ketamine on proliferation and ferroptosis of liver cancer.

Methods: Cell viability and proliferation were detected by cell counting kit 8 (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) assay. Ferroptosis was determined by levels of Fe, lipid reactive oxygen species (ROS), and malondialdehyde (MDA). RNA levels of lncPVT1, miR-214-3p, and glutathione peroxidase 4 (GPX4) were checked by real-time PCR assay. Clinical liver tumor samples were collected to detect the levels of long noncoding RNA lncPVT1, miR-214-3p, and GPX4, and their correlation was evaluated by Pearson comparison test. Luciferase reporter gene assay and RNA pulldown were conducted to determine the binding between lncPVT1, miR-214-3p, and GPX4 3'UTR.

Results: Ketamine significantly suppressed viability and proliferation of liver cancer cells both in vitro and in vivo, as well as stimulated ferroptosis, along with decreased expression of lncPVT1 and GPX4. LncPVT1 directly interacted with miR-214-3p to impede its role as a sponge of GPX4. Depletion of lncPVT1 accelerated the ferroptosis of live cancer cells, whereas miR-214-3p inhibition and GPX4 overexpression reversed this effect. Ketamine-induced cell growth suppression and ferroptosis were also suppressed by miR-214-3p inhibition and GPX4 overexpression.

Conclusion: In this work, we determined that ketamine suppressed viability of liver cancer cells and induced ferroptosis and identified the possible regulatory mechanism of lncPVT1/miR-214-3p/GPX4 axis.
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http://dx.doi.org/10.2147/DDDT.S332847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458041PMC
September 2021

Identification of the prognostic value of a 2-gene signature of the WNT gene family in UCEC using bioinformatics and real-world data.

Cancer Cell Int 2021 Sep 26;21(1):516. Epub 2021 Sep 26.

Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The WNT gene family plays an important role in the occurrence and development of malignant tumors, but its involvement has not been systematically analyzed in uterine corpus endometrial carcinoma (UCEC). This study aimed to evaluate the prognostic value of the WNT gene family in UCEC.

Methods: Pan-cancer transcriptome data of the UCSC Xena database and Genotype-Tissue Expression (GTEx) normal tissue data were downloaded to analyze the expression and prognosis of 19 WNT family genes in UCEC. A cohort from The Cancer Genome Atlas-Uterine Corpus Endometrial Carcinoma (TCGA-UCEC) was used to analyze the expression of the WNT gene family in different immune subtypes and clinical subgroups. The STRING database was used to analyze the interaction of the WNT gene family and its biological function. Univariate Cox regression analysis and Lasso cox analysis were used to identify the genes associated with significant prognosis and to construct multi signature prognosis model. An immunohistochemical assay was used to verify the predictive ability of the model. Risk score and the related clinical features were used to construct a nomogram.

Results: The expression levels of WNT2, WNT3, WNT3A, WNT5A, WNT7A, and WNT10A were significantly different among different immune subtypes and correlated with TP53 mutation. According to the WNT family genes related to the prognosis of UCEC, UCEC was classified into two subtypes (C1, C2). The prognosis of subtype C1 was significantly better than that of subtype C2. A 2-gene signature (WNT2 and WNT10A) was constructed and the two significantly prognostic groups can be divided based on median Risk score. These results were verified using real-world data, and the nomogram constructed using clinical features and Risk score had good prognostic ability.

Conclusions: The 2-gene signature including WNT2 and WNT10A can be used to predict the prognosis of patients with UCEC, which is important for clinical decision-making and individualized therapy for patients with UCEC.
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http://dx.doi.org/10.1186/s12935-021-02215-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474865PMC
September 2021

Differentiated expression of long non-coding RNA-small nucleolar RNA host gene 8 in atherosclerosis and its molecular mechanism.

Bioengineered 2021 12;12(1):7167-7176

Department of Emergency, Yidu Central Hospital of Weifang, Weifang, Shandong China.

Atherosclerosis (AS) is one of the most common cardiovascular diseases, and the incidence is increasing year by year. Many studies have shown that long non-coding RNA plays a vital role in the pathogenesis of AS. This study aimed to explore the role and mechanism of lncRNA-small nucleolar RNA host gene 8 (SNHG8) in AS. The expressions of serum lncSNHG8 and miR-224-3p were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The diagnostic meaning of lncSNHG8 in AS was estimated by Receiver operating characteristic (ROC) curve. The correlation between lncSNHG8 and various clinical indicators, as well as miR-244-3p was evaluated by Pearson correlation coefficient analysis. Cell proliferation and migration were estimated by cell counting kit-8 (CCK-8) and Transwell assay. The interaction between lncSNHG8 and miR-224-3p was proved by luciferase reporter gene assay. The expression level of lncSNHG8 was increased in AS patients, while miR-224-3p expression was decreased. The ROC curve indicated that lncSNHG8 with high serum expression had the ability to distinguish AS. Pearson correlation coefficient exhibited that the level of miR-224-3p was negatively correlated with the level of lncSNHG8. The results of cell experiments indicated that inhibition of the expression of lncSNHG8 significantly inhibited the proliferation and migration of vascular smooth muscle cells (VSMCs). Luciferase reporter gene experiments confirmed that there was a target relationship between lncSNHG8 and miR-224-3p. In conclusion, lncSNHG8 had high diagnostic value for AS. It promoted the proliferation and migration of VSMCs by adsorption and inhibition of miR-224-3p.
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http://dx.doi.org/10.1080/21655979.2021.1979441DOI Listing
December 2021

Application of artificial intelligence in preoperative imaging of hepatocellular carcinoma: Current status and future perspectives.

World J Gastroenterol 2021 Aug;27(32):5341-5350

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor in China. Preoperative diagnosis of HCC is challenging because of atypical imaging manifestations and the diversity of focal liver lesions. Artificial intelligence (AI), such as machine learning (ML) and deep learning, has recently gained attention for its capability to reveal quantitative information on images. Currently, AI is used throughout the entire radiomics process and plays a critical role in multiple fields of medicine. This review summarizes the applications of AI in various aspects of preoperative imaging of HCC, including segmentation, differential diagnosis, prediction of histopathology, early detection of recurrence after curative treatment, and evaluation of treatment response. We also review the limitations of previous studies and discuss future directions for diagnostic imaging of HCC.
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http://dx.doi.org/10.3748/wjg.v27.i32.5341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409162PMC
August 2021

Improved Whole-Genome Sequence of Fusarium meridionale, the Fungal Pathogen Causing Fusarium Head Blight in Rice.

Mol Plant Microbe Interact 2021 Sep 17. Epub 2021 Sep 17.

Institute of Food Safey and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China;

Members of the Fusarium graminearum species complex (FGSC) cause extensive yield losses in cereal production worldwide and food safety concerns due to the accumulation of Fusarium toxins in infected grains. Among these pathogens, F. meridionale is responsible for Fusarium head blight of wheat and rice, ear and stalk rot of maize, and pod blight of soybean. Here, we present an improved genome assembly of F. meridionale strain SR5 isolated from rice in China based on PacBio long-read sequencing and Illumina short-read sequencing technology. The assembled genome of SR5 has a total size of 36.82 Mb, an N50 scaffold length of 7.82 Mb, 9 scaffolds and encodes 12,409 predicted genes. These high-quality data expand FGSC genomic resources and provide a valuable resource for better understanding their genetic diversity and the molecular basis of pathogenesis, which will facilitate the development of an effective control strategy.
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http://dx.doi.org/10.1094/MPMI-07-21-0182-ADOI Listing
September 2021

A Hybrid SSA and SMA with Mutation Opposition-Based Learning for Constrained Engineering Problems.

Comput Intell Neurosci 2021 7;2021:6379469. Epub 2021 Sep 7.

School of Education and Music, Sanming University, Sanming 365004, China.

Based on Salp Swarm Algorithm (SSA) and Slime Mould Algorithm (SMA), a novel hybrid optimization algorithm, named Hybrid Slime Mould Salp Swarm Algorithm (HSMSSA), is proposed to solve constrained engineering problems. SSA can obtain good results in solving some optimization problems. However, it is easy to suffer from local minima and lower density of population. SMA specializes in global exploration and good robustness, but its convergence rate is too slow to find satisfactory solutions efficiently. Thus, in this paper, considering the characteristics and advantages of both the above optimization algorithms, SMA is integrated into the leader position updating equations of SSA, which can share helpful information so that the proposed algorithm can utilize these two algorithms' advantages to enhance global optimization performance. Furthermore, Levy flight is utilized to enhance the exploration ability. It is worth noting that a novel strategy called mutation opposition-based learning is proposed to enhance the performance of the hybrid optimization algorithm on premature convergence avoidance, balance between exploration and exploitation phases, and finding satisfactory global optimum. To evaluate the efficiency of the proposed algorithm, HSMSSA is applied to 23 different benchmark functions of the unimodal and multimodal types. Additionally, five classical constrained engineering problems are utilized to evaluate the proposed technique's practicable abilities. The simulation results show that the HSMSSA method is more competitive and presents more engineering effectiveness for real-world constrained problems than SMA, SSA, and other comparative algorithms. In the end, we also provide some potential areas for future studies such as feature selection and multilevel threshold image segmentation.
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http://dx.doi.org/10.1155/2021/6379469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440113PMC
September 2021

Preparation of a Water-Soluble Zn Cd S Quantum Dot Photocatalyst at Room Temperature Assisted by 3-Mercaptopropionic Acid.

Chem Asian J 2021 Sep 15. Epub 2021 Sep 15.

School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, P. R. China.

An effective path to synthesize Zn Cd S quantum dots (ZCS QDs) in aqueous phase at room temperature has remained relatively unexplored. Here, we developed a room-temperature, aqueous-phase approach to ZCS QDs, using 3-mercaptopropionic acid (MPA) to adjust the pH of the reaction precursor solution to regulate the competition between sulfur source and hydroxyl group, and realize the large-scale preparation of water-soluble ZCS QDs photocatalyst at room temperature. Without recombination with other materials, and only by regulating the ratio of pH, excess sulfur sources and Zn/Cd, the photocatalytic degradation of rhodamine B (RhB) can reach 98% within 1 min, showing high photocatalytic activity. ZCS QDs show high stability and recoverability, and are expected to be able to deal with organic pollutants on a large scale. This study provides a new idea for the preparation of other QDs at room temperature.
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http://dx.doi.org/10.1002/asia.202100904DOI Listing
September 2021
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