Publications by authors named "Shuang Liu"

1,721 Publications

  • Page 1 of 1

Rare case of upper gastrointestinal hemorrhage due to accessory spleen: A case report.

Medicine (Baltimore) 2022 Aug;101(31):e29636

Department of Nephrology, Suining Central Hospital, Suining, Sichuan Province, China.

Rationale: Upper gastrointestinal hemorrhage (UGIH) is defined as hemorrhage originating from the gastrointestinal tract proximal to the ligament of Treitz. The causes of UGIH include esophagitis, gastritis, peptic ulcers, Mallory-Weiss syndrome, and cancer. However, a rare cause of UGIH, such as an accessory spleen, may lead to serious complications if left untreated and can sometimes be very difficult to diagnose preoperatively.

Patient Concerns: An 18-year-old man was admitted to the Department of Gastroenterology of our hospital due to "repeated black stool for 2 months with aggravation, accompanied by hematemesis for 9 days." He denied any history of hepatitis, trauma, or surgery.

Diagnosis: Laboratory evaluation revealed severe anemia (hemoglobin, 6.4 g/dL). Computed tomography revealed a mass measuring 127 mm in its largest dimension, located in the upper left abdomen, with varicose veins in the gastric fundus. Moreover, distended blue-purple tortuous veins were observed by gastroscopy in the gastric fundus. We believed the mass was likely an abnormally proliferating accessory spleen; however, the causes of severe anemia and gastrointestinal hemorrhage were unknown.

Interventions: After discussion in a multidisciplinary conference, the mass was completely resected laparoscopically, and the subserosal veins in the gastric fundus were sutured using absorbable threads.

Outcomes: After the surgery, the patient recovered uneventfully without any complications. Clinicopathological examination showed that the mass was chronic congestive splenomegaly. Gastrointestinal hemorrhage secondary to an abnormally proliferating accessory spleen was confirmed as the diagnosis. Laboratory evaluation revealed hemoglobin at 12.1 g/dL 2 months after surgery. At the 12-month follow-up, the patient showed no recurrence of gastrointestinal hemorrhage.

Lessons: UGIH caused by accessory spleen is extremely rare. This entity should be considered in differential diagnosis of gastrointestinal hemorrhage. Surgical intervention is necessary for timely diagnosis and treatment in case of gastrointestinal hemorrhage in critical clinical situations.
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http://dx.doi.org/10.1097/MD.0000000000029636DOI Listing
August 2022

Target nursing care on anxiety and depression in patients with gallbladder cancer during perioperative period.

Medicine (Baltimore) 2022 Aug;101(31):e29883

First Ward of General Surgery Department, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, China.

Background: This study retrospectively investigated the effects of target nursing care (TNC) on anxiety and depression in patients with gallbladder cancer (GBC) during the perioperative period.

Methods: This retrospective study analyzed the data of 80 patients with GBC during perioperative period. These records were divided into an intervention group (n = 40) or a control group (n = 40). All 80 patient records in both groups were administered routine nursing care (RNC). The patients in the intervention group also underwent TNC. The primary outcomes were depression (measured using the Hamilton Depression Scale, HAMD) and anxiety (assessed using the Hamilton Anxiety Scale, HAMA). The secondary outcomes were quality of life (assessed using the 36-Item Short Form Health Survey, SF-36) and adverse events. We collected and analyzed the outcome data before and after treatment.

Results: After treatment, patients in the intervention group showed more promising effects on depression (HAMD, P < .01) and anxiety (HAMA, P < .01) than those in the control group did. However, there were no significant differences in the quality of life before and after treatment. No TNC- or RNC-associated adverse events were reported in patient records.

Conclusion: This study found that TNC was more effective than RNC in relieving depression and anxiety. Future studies should be conducted to validate the present findings.
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http://dx.doi.org/10.1097/MD.0000000000029883DOI Listing
August 2022

c-Myc plays a critical role in the antileukemic activity of the Mcl-1-selective inhibitor AZD5991 in acute myeloid leukemia.

Apoptosis 2022 Aug 9. Epub 2022 Aug 9.

Department of Oncology, Wayne State University School of Medicine, 421 E. Canfield, 48201, Detroit, MI, USA.

Acute myeloid leukemia (AML) is an aggressive disease with a low 5-year overall survival rate of 29.5%. Thus, more effective therapies are in need to prolong survival of AML patients. Mcl-1 is overexpressed in AML and is associated with poor prognosis, representing a promising therapeutic target. The oncoprotein c-Myc is also overexpressed in AML and is a significant prognostic factor. In addition, Mcl-1 is required for c-Myc induced AML, indicating that c-Myc-driven AML harbors a Mcl-1 dependency and co-targeting of Mcl-1 and c-Myc represents a promising strategy to eradicate AML. In this study, we investigated the role of c-Myc in the antileukemic activity of Mcl-1 selective inhibitor AZD5991 and the antileukemic activity of co-targeting of Mcl-1 and c-Myc in preclinical models of AML. We found that c-Myc protein levels negatively correlated with AZD5991 ECs in AML cell lines and primary patient samples. AZD5991 combined with inhibition of c-Myc synergistically induced apoptosis in AML cell lines and primary patient samples, and cooperatively targeted leukemia progenitor cells. AML cells with acquired resistance to AZD5991 were resensitized to AZD5991 when c-Myc was inhibited. The combination also showed promising and synergistic antileukemic activity in vitro against AML cell lines with acquired resistance to the main chemotherapeutic drug AraC and primary AML cells derived from a patient at relapse post chemotherapy. The oncoprotein c-Myc represents a potential biomarker of AZD5991 sensitivity and inhibition of c-Myc synergistically enhances the antileukemic activity of AZD5991 against AML.
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http://dx.doi.org/10.1007/s10495-022-01756-7DOI Listing
August 2022

Tuning crystal-phase of bimetallic single-nanoparticle for catalytic hydrogenation.

Nat Commun 2022 Aug 5;13(1):4559. Epub 2022 Aug 5.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Bimetallic nanoparticles afford geometric variation and electron redistribution via strong metal-metal interactions that substantially promote the activity and selectivity in catalysis. Quantitatively describing the atomic configuration of the catalytically active sites, however, is experimentally challenged by the averaging ensemble effect that is caused by the interplay between particle size and crystal-phase at elevated temperatures and under reactive gases. Here, we report that the intrinsic activity of the body-centered cubic PdCu nanoparticle, for acetylene hydrogenation, is one order of magnitude greater than that of the face-centered cubic one. This finding is based on precisely identifying the atomic structures of the active sites over the same-sized but crystal-phase-varied single-particles. The densely-populated Pd-Cu bond on the chemically ordered nanoparticle possesses isolated Pd site with a lower coordination number and a high-lying valence d-band center, and thus greatly expedites the dissociation of H over Pd atom and efficiently accommodates the activated H atoms on the particle top/subsurfaces.
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http://dx.doi.org/10.1038/s41467-022-32274-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355964PMC
August 2022

Nanogels as Novel Nanocarrier Systems for Efficient Delivery of CNS Therapeutics.

Front Bioeng Biotechnol 2022 19;10:954470. Epub 2022 Jul 19.

Department of Anatomy, College of Basic Medicine Sciences, Jilin University, Changchun, China.

Nanogels have come out as a great potential drug delivery platform due to its prominently high colloidal stability, high drug loading, core-shell structure, good permeation property and can be responsive to environmental stimuli. Such nanoscopic drug carriers have more excellent abilities over conventional nanomaterials for permeating to brain parenchyma and . Nanogel-based system can be nanoengineered to bypass physiological barriers via non-invasive treatment, rendering it a most suitable platform for the management of neurological conditions such as neurodegenerative disorders, brain tumors, epilepsy and ischemic stroke, etc. Therapeutics of central nervous system (CNS) diseases have shown marked limited site-specific delivery of CNS by the poor access of various drugs into the brain, due to the presences of the blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier (BCSFB). Hence, the availability of therapeutics delivery strategies is considered as one of the most major challenges facing the treatment of CNS diseases. The primary objective of this review is to elaborate the newer advances of nanogel for CNS drugs delivery, discuss the early preclinical success in the field of nanogel technology and highlight different insights on its potential neurotoxicity.
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http://dx.doi.org/10.3389/fbioe.2022.954470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343834PMC
July 2022

AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis.

Nat Commun 2022 Aug 3;13(1):4499. Epub 2022 Aug 3.

State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Centre, Institute of Artificial Intelligence Biomedicine, School of Life Sciences, Nanjing University, Nanjing, 210023, China.

Macrocyclization is an important process that affords morphed scaffold in biosynthesis of bioactive natural products. Nature has adapted diverse biosynthetic strategies to form macrocycles. In this work, we report the identification and characterization of a small enzyme AvmM that can catalyze the construction of a 16-membered macrocyclic ring in the biosynthesis of alchivemycin A (1). We show through in vivo gene deletion, in vitro biochemical assay and isotope labelling experiments that AvmM catalyzes tandem dehydration and Michael-type addition to generate the core scaffold of 1. Mechanistic studies by crystallography, DFT calculations and MD simulations of AvmM reveal that the reactions are achieved with assistance from the special tenuazonic acid like moiety of substrate. Our results thus uncover an uncharacterized macrocyclization strategy in natural product biosynthesis.
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http://dx.doi.org/10.1038/s41467-022-32088-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349299PMC
August 2022

Mast cells promote viral entry of SARS-CoV-2 via formation of chymase/spike protein complex.

Eur J Pharmacol 2022 Jul 31;930:175169. Epub 2022 Jul 31.

Department of Pharmacology, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 791-0295, Japan.

The pulmonary pathological findings associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) result from the release of multiple proinflammatory cytokines, which causes the subsequential damage of the lungs. The current study was undertaken to investigate the responses of mast cells to viral inoculation and their contribution to host defenses from the point of view of viral entry. Pseudovirions, in which the spike glycoprotein of SARS-CoV-2 was incorporated, triggered activation of mast cells, and a mast cell-derived chymase, MCP2, formed a complex with spike protein, which promoted protease-dependent viral entry. According to the quantification results of viral entry, 10 μM quercetin, a mast cell stabilizer, potentially potently inhibited 41.3% of viral entry, while 100 μM chymostatin, which served as a chymase inhibitor, suppressed 52.1% of viral entry, compared to non-treated cells. Study using mast cell-deficient mice showed that the absence of mast cells may influence early viral loading in the upper respiratory tract, which consequently increases the risk of viral invasion into the lower respiratory system. Furthermore, mast cell-deficient mice exhibited ongoing infection in the late phase post-viral inoculation, while clearance of virus-positive cells was observed in wild-type mice. In conclusion, mast cells act as a multifaceted immune modulator that is equipped with both protective effects and pathogenic influences on viral entry of SARS-CoV-2. The utility of mast cell stabilizers and chymase inhibitors in the treatment of SARS-CoV-2-induced acute respiratory syndrome should be optimized regarding the infection stage and the risk of cytokine storm.
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http://dx.doi.org/10.1016/j.ejphar.2022.175169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339018PMC
July 2022

Comprehensive characterization of enhancer RNA in hepatocellular carcinoma reveals three immune subtypes with implications for immunotherapy.

Mol Ther Oncolytics 2022 Sep 6;26:226-244. Epub 2022 Jul 6.

Department of Liver Surgery, Sun Yat-sen University Cancer Center, Guangzhou, China.

Hepatocellular carcinoma (HCC) is highly heterogeneous. Molecular subtyping for guiding immunotherapy is warranted. Previous studies have indicated that enhancer RNAs (eRNAs) are involved in tumor heterogeneity and immune infiltration. However, the eRNA landscape and its correlation with immune infiltration in HCC remain unknown. Here we first revealed the genome-wide eRNA landscape in two HCC cohorts. Then we divided individuals with HCC into three immune-related clusters (C1, C2, and C3) based on eRNA expression profiles. The prognosis, biological properties, immune infiltration, clinical features, genomic features, and drug response were analyzed. C1 was enriched in immune infiltration and potentially sensitive to immune checkpoint inhibitors (ICIs). C2 displayed features of immune depletion, high proliferation activity, malignant clinical features, and the worst prognosis. C2 may benefit from targeted therapy. C3 presented moderate immune infiltration, metabolism-related signatures, and the best prognosis. Transarterial chemoembolization (TACE) may be effective for C3. Finally, we constructed a 51-eRNA classifier for subtype prediction and validated its efficacy in The Cancer Genome Atlas (TCGA) cohort and Sun Yat-sen University Cancer Center (SYSUCC) cohort. Our results provide a novel method for immune classification of HCC, shed new light on tumor heterogeneity, and may aid in HCC immunotherapy.
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http://dx.doi.org/10.1016/j.omto.2022.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310078PMC
September 2022

Fresh Frozen Plasma in Cases of Acute Upper Gastrointestinal Bleeding Does Not Improve Outcomes.

Front Med (Lausanne) 2022 14;9:934024. Epub 2022 Jul 14.

Emergency Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Background: Blood products are commonly transfused in patients with acute upper gastrointestinal bleeding (UGIB). There exists considerable practice variation and less evidence to guide fresh frozen plasma transfusion in patients with UGIB. The aim of this study was to explore any association between fresh frozen plasma transfusion following acute UGIB and clinical outcomes.

Methods: This was a prospective, observational, multicenter study conducted at 20 tertiary hospitals in China. Patients with acute UGIB with an international normalized ratio ≤ 2.0 at emergency department admission were included. Multivariate logistic regression models were used to examine and quantify any clinical associations.

Results: A total of 976 patients (61.57 ± 15.79 years old, 73.05% male) were included, of whom 17.42% received fresh frozen plasma transfusion. The overall 90-day mortality and rebleeding rates were 10.20 and 12.19%, respectively. After adjusting for confounding factors, transfusion of fresh frozen plasma during hospitalization was associated with higher 90-day mortality [odd ratio (OR), 2.36; 95% confidence interval (CI), 1.36-4.09; = 0.002] but not rebleeding (OR, 1.5; 95% CI; 0.94-2.54; = 0.085). In a subgroup analysis, patients with an international normalized ratio <1.5 who were treated with fresh frozen plasma were associated with both significantly higher 90-day mortality (OR, 2.78; 95% CI, 1.49-5.21; = 0.001) and rebleeding (OR, 2.02; 95% CI, 1.16-3.52; = 0.013), whereas in patients with an international normalized ratio between 1.5 and 2, we did not find any significant correlation.

Conclusion: This study found an association between fresh frozen plasma transfusion following acute UGIB and elevated 90-day mortality. Both 90-day mortality and rebleeding risk were significantly higher in patients with an international normalized ratio < 1.5. Fresh frozen plasma transfusion in acute UGIB does not improve the poor outcomes (Chinese Clinical Trial registry, Number ChiCTR1900028676).
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http://dx.doi.org/10.3389/fmed.2022.934024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330331PMC
July 2022

Fabrication and Characterization of Degradable Crop-Straw-Fiber Composite Film Using In Situ Polymerization with Melamine-Urea-Formaldehyde Prepolymer for Agricultural Film Mulching.

Materials (Basel) 2022 Jul 26;15(15). Epub 2022 Jul 26.

Faculty of Engineering, Northeast Agricultural University, Haerbin 150030, China.

Soil mulch composite films composed of biodegradable materials are being increasingly used in agriculture. In this study, mulch films based on wheat straw fiber and an environmentally friendly modifier were prepared via in situ polymerization and tested as the ridge mulch for crops. The mechanical properties of the straw fiber film were significantly enhanced by the modification. In particular, the films exhibited a noticeable increase in dry and wet tensile strength from 2.35 to 4.15 and 0.41 to 1.51 kN/m, respectively, with increasing filler content from 0% to 25%. The contact angle of the straw also showed an improvement based on its hydrophilicity. The crystallinity of the modified film was higher than that of the unmodified film and increased with modifier content. The changes in chemical interaction of the straw fiber film were determined by Fourier transform infrared spectroscopy, and the thermal stability of the unmodified film was improved by in situ polymerization. Scanning electron microscopy images indicated that the modifier was uniformly dispersed in the fiber film, resulting in an improvement in its mechanical properties. The modified straw fiber films could be degraded after mulching for approximately 50 days. Overall, the superior properties of the modified straw fiber film lend it great potential for agricultural application.
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http://dx.doi.org/10.3390/ma15155170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331358PMC
July 2022

Lactate promotes metastasis of normoxic colorectal cancer stem cells through PGC-1α-mediated oxidative phosphorylation.

Cell Death Dis 2022 Jul 27;13(7):651. Epub 2022 Jul 27.

Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Uneven oxygen supply in solid tumors leads to hypoxic and normoxic regions. Hypoxic cells exhibit increased secretion of lactate, which creates an acidic tumor microenvironment (TME). This acidic TME is positively associated with tumor metastasis. Despite the increased metastatic capacity of hypoxic cells, they are located relatively further away from the blood vessels and have limited access to the circulatory system. Studies have shown that cancer stem cells (CSCs) are enriched for tumor metastasis-initiating cells and generally undergo aerobic respiration, which could be enhanced by lactate. We therefore hypothesized that TME-derived lactate may promote the metastasis of normoxic CSCs. In the present study, the abundance of hypoxic and normoxic CSCs was analyzed in primary CRC tumors. It was found that the proportion of normoxic CSCs was positively associated with tumor stage. Using two human CRC cell lines, LoVo and SW480, and a patient-derived xenograft (XhCRC), it was found that treatment with lactate promoted normoxic CSC metastasis. Metabolism analysis indicated that, upon treatment with lactate, oxidative phosphorylation (OXPHOS) activity in normoxic CSCs was enhanced, whereas hypoxic CSCs were rarely altered. At the molecular level, the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), a master regulator of lactate oxidation, was found to be elevated in normoxic CSCs. Furthermore, PGC-1α knockdown markedly reduced the metastatic potential of normoxic CSCs. Notably, both the PGC-1α-mediated OXPHOS activity and metastatic potential were impaired when hypoxia-inducible factor-1α (HIF-1α) was activated in normoxic CSCs. Together, these findings provide a therapeutic strategy against tumor metastasis through the targeting of PGC-1α and, thus, the suppression of lactate-feeding OXPHOS in normoxic CSCs may improve the therapeutic benefit of patients with cancer, particularly CRC.
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http://dx.doi.org/10.1038/s41419-022-05111-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329320PMC
July 2022

Conformational Transition-Driven Self-Folding Hydrogel Based on Silk Fibroin and Gelatin for Tissue Engineering Applications.

Macromol Biosci 2022 Jul 27:e2200189. Epub 2022 Jul 27.

Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing, 401331, P. R. China.

Self-folding is a rapidly evolving method for converting flat objects into three-dimensional (3D) structures. However, because there are few materials with suitable properties, the application of self-folding in tissue engineering has been hindered greatly. Herein, a novel self-folding hydrogel using  conformational transition mechanism is developed by employing  photocrosslinkable silk fibroin and gelatin composite hydrogel. It is hypothesized that differences in the amount of beta-sheet (β-sheet) formation between the upper and lower layers will supply additional folding stress and drive the self-folding behavior of a bilayer patch, which can improve the mechanical properties and long-term stability of the self-folded structure. In this study, the impact of various proportions of β-sheets in composite hydrogels on their swelling, mechanics, and internal microstructures are investigated. Subsequently, the folding process parameters are optimized, and diffusion through the folded tubular structure is studied with a perfusion test. Finally, it is proven that the self-folding hydrogel system is cytocompatible and can be utilized to build a 3D coculture system of "endothelial cells-smooth muscle cells". These findings suggest that the self-folding hydrogel can be a promising candidate for applications in blood vessel tissue engineering and regenerative medicine.
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http://dx.doi.org/10.1002/mabi.202200189DOI Listing
July 2022

Development of a Novel RT-qPCR Detecting Method of Covert Mortality Nodavirus (CMNV) for the National Proficiency Test in Molecular Detection.

Viruses 2022 07 5;14(7). Epub 2022 Jul 5.

College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

Covert mortality nodavirus (CMNV), the pathogen of viral covert mortality disease (VCMD), has caused serious economic losses of shrimp aquaculture in Southeast Asian countries and China in the past decade. In view of that the rapid and accurate laboratory detection of CMNV plays a major role in the effective control of the spread of VCMD. The national proficiency test (NPT) for the detection of covert mortality nodavirus (CMNV) started in China from 2021. In this study, a novel TaqMan real-time reverse transcription quantitative PCR (RT-qPCR) detection method for CMNV with higher sensitivity than previous reports was established based on specific primers and probe designing from the conserved regions of the CMNV coat protein gene for using molecular detection of CMNV in NPT. The optimized RT-qPCR reaction program was determined as reverse transcription at 54.9 °C for 15 min and denaturation at 95 °C for 1 min, followed by 40 cycles including denaturation at 95 °C for 10 s, and annealing and extension at 54.9 °C for 25 s. The detection limit of the newly developed RT-qPCR method was determined to be as low as 2.15 copies of CMNV plasmids template per reaction, with the correlation coefficient (R) at above 0.99. The new method showed no cross reaction with the six common aquatic animal pathogens and could be finished in one hour, which represents a rapid detection method that can save 50% detection time versus the previously reported assay. The CMNV TaqMan probe based RT-qPCR method developed in present study supplies a novel sensitive and specific tool for both the rapid diagnosing and quantitating of CMNV in NPT activities and in the farmed crustaceans, and will help practitioners in the aquaculture industry to prevent and control VCMD effectively.
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http://dx.doi.org/10.3390/v14071475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319686PMC
July 2022

Transplacental Transfer of Maternal Antibody against SARS-CoV-2 and Its Influencing Factors: A Review.

Vaccines (Basel) 2022 Jul 6;10(7). Epub 2022 Jul 6.

Department of Epidemiology, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.

Since the beginning of the coronavirus disease 2019 (COVID-19) outbreak, the disease has rapidly become a global threat. The constant emergence of new variants has increased the difficulty of controlling this disease. Vaccination is still considered the most effective method to prevent COVID-19. Vaccination has expanded to include children aged 3-17 years old, and some countries have lowered the age of vaccination to 6 months (for example, the United States). However, children under 3 years old are still not able to be vaccinated in most countries. In this study, we summarize the COVID-19 vaccination status in pregnant women, comprehensively elaborate on the status of maternal immune response and maternal antibody transfer after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and vaccination, and further analyze the possible influencing factors of maternal antibody transfer according to the currently available evidence on the topic. It was concluded that pregnant women develop an immune response and produce antibodies that can be transmitted through the placenta after vaccination, but more data are needed to determine the transfer rate and duration of these maternal antibodies and potential factors. The results provide a scientific basis for studying the protective effect of maternal antibodies on infants, formulating a vaccination strategy for pregnant women, and preventing SARS-CoV-2 infection in infants.
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http://dx.doi.org/10.3390/vaccines10071083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318929PMC
July 2022

Molecular Dynamic Simulation of Defective Graphene Nanoribbons for Tension and Vibration.

Nanomaterials (Basel) 2022 Jul 14;12(14). Epub 2022 Jul 14.

Beijing Key Laboratory of Nonlinear Vibrations and Strength of Mechanical Structures, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing 100124, China.

As deformation and defects are inevitable during the manufacture and service of graphene resonators, comprehensive molecular dynamic (MD) simulations are performed to investigate the vibrational properties of the defective single-layer graphene sheets (SLGSs) during tension. Perfect SLGSs, SLGSs with single vacancy, SLGSs with low-concentration vacancies, and SLGSs with high-concentration vacancies are considered, respectively. The frequencies of the perfect and defective SLGSs at different stretching stages are investigated in detail. The effects of different external forces are also taken into account to study the vibration properties of the defective SLGSs. Results show that the perfect and defective SLGSs both successively perform four stages, i.e., the elastic stage, the yield stage, the hardening stage, and the fracture stage during stretching, and the elastic properties of the SLGSs are insensitive to the vacancy defects, while the ultimate strain is noticeably reduced by the vacancies. The single vacancy has no effect on the vibration properties of SLGS, while the frequency decreases with the increasing vacancy concentration for SLGS at the elastic stage. The frequency of yielded SLGS with a certain vacancy concentration is almost constant even with a varying external force.
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http://dx.doi.org/10.3390/nano12142407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315857PMC
July 2022

Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo.

Int J Mol Sci 2022 Jul 12;23(14). Epub 2022 Jul 12.

The Sericultural and Silk Research Institute, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.

Mulberry ( L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food.
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http://dx.doi.org/10.3390/ijms23147694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318041PMC
July 2022

Hub Genes, Diagnostic Model, and Predicted Drugs Related to Iron Metabolism in Alzheimer's Disease.

Front Aging Neurosci 2022 7;14:949083. Epub 2022 Jul 7.

Shanghai Key Laboratory of Molecular Imaging, Zhoupu Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, China.

Alzheimer's disease (AD), the most common neurodegenerative disease, remains unclear in terms of its underlying causative genes and effective therapeutic approaches. Meanwhile, abnormalities in iron metabolism have been demonstrated in patients and mouse models with AD. Therefore, this study sought to find hub genes based on iron metabolism that can influence the diagnosis and treatment of AD. First, gene expression profiles were downloaded from the GEO database, including non-demented (ND) controls and AD samples. Fourteen iron metabolism-related gene sets were downloaded from the MSigDB database, yielding 520 iron metabolism-related genes. The final nine hub genes associated with iron metabolism and AD were obtained by differential analysis and WGCNA in brain tissue samples from GSE132903. GO analysis revealed that these genes were mainly involved in two major biological processes, autophagy and iron metabolism. Through stepwise regression and logistic regression analyses, we selected four of these genes to construct a diagnostic model of AD. The model was validated in blood samples from GSE63061 and GSE85426, and the AUC values showed that the model had a relatively good diagnostic performance. In addition, the immune cell infiltration of the samples and the correlation of different immune factors with these hub genes were further explored. The results suggested that these genes may also play an important role in immunity to AD. Finally, eight drugs targeting these nine hub genes were retrieved from the DrugBank database, some of which were shown to be useful for the treatment of AD or other concomitant conditions, such as insomnia and agitation. In conclusion, this model is expected to guide the diagnosis of patients with AD by detecting the expression of several genes in the blood. These hub genes may also assist in understanding the development and drug treatment of AD.
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http://dx.doi.org/10.3389/fnagi.2022.949083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300955PMC
July 2022

Evaluating the effects of mitochondrial autophagy flux on ginsenoside Rg2 for delaying D-galactose induced brain aging in mice.

Phytomedicine 2022 Sep 16;104:154341. Epub 2022 Jul 16.

College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; National & Local Joint Engineering Research Center for Ginseng Breeding and Development, Changchun 130118, China. Electronic address:

Background: Aging is an inevitable gradual process of the body, which can cause dysfunction or degeneration of the nervous or immune system, thus becoming a critical pathogenic factor inducing neurodegenerative diseases. Previous reports have confirmed that saponins (ginsenosides) derived from Panax ginseng. C.A. Meyer exerted obvious memory-enhancing and anti-aging effects, and the simpler the structure of ginsenosides, the better the biological activity. Ginsenoside Rg2 (Rg2) is a prominent and representative panaxatriol-type ginsenoside produced during ginseng processing, which has been reported to have pretty good neuroprotective activity.

Purpose: The work was aimed at exploring the therapeutic effects and possible molecular mechanisms of Rg2 by establishing the subacute brain aging model induced by D-galactose (D-gal) in mice.

Methods: The anti-aging activity of G-Rg2 (10, 20 mg/kg for 4 weeks) was assessed using the D-gal induced brain aging model (800 mg/kg for 8 weeks). The Morris water maze (MWM) and histopathological analysis were used to evaluate the cognitive function and pathological changes of the brain in mice, respectively. The protein expression levels of p53, p21, p16ink4α, IL-6, CDK4, ATG3, ATG5, ATG7, LC3, p62, LAMP2, and TFEB were quantified through western blot analysis. The degree of mitochondrial damage and the number of mitochondrial autophagolysosomes in hippocampal neurons were monitored using TEM analysis.

Results: The results showed that Rg2 could significantly restore D-gal-induced impaired memory function, choline dysfunction, and redox system imbalance in mice. Rg2 treatment also considerably decreased the over-expression of aging-related proteins such as p53/p21/p16ink4α induced by D-galactose, which demonstrated that Rg2 possessed good anti-aging activity. Meanwhile, Rg2 could evidently reduce the pathological changes caused by D-gal exposure. Moreover, the results from transmission electron microscopy and western blot analysis indicated that Rg2 could delay the brain aging induced by D-gal in mice via promoting the degradation of the autophagy substrate p62 while increasing the protein expression level of LAMP2/TFEB to maintain mitochondrial function.

Conclusion: These results indicate that Rg2 could postpone brain aging by increasing mitochondrial autophagy flux to maintain mitochondrial function, which greatly enriched the research on the pharmacological activity of ginsenosides for delaying brain aging.
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http://dx.doi.org/10.1016/j.phymed.2022.154341DOI Listing
September 2022

Perception of strong social norms during the COVID-19 pandemic is linked to positive psychological outcomes.

BMC Public Health 2022 07 22;22(1):1403. Epub 2022 Jul 22.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, 16 Lincui Road, Chaoyang District, Beijing, 100101, China.

Social norms can coordinate individuals and groups during collective threats. Pandemic-related social norms (e.g., wearing masks, social distancing) emerged to curb the spread of COVID-19. However, little is known about the psychological consequences of the emerging norms. We conducted three experiments cross-culturally, during the early period of the COVID-19 pandemic in China (Study 1), the recovery period in China (Study 2), and the severe period in the United States and Canada (Study 3). Across the three studies, we first distinguished the opposite effects of social norms and risk perception on individuals' psychological characteristics during the COVID-19 pandemic and further revealed that individuals who perceived stronger pandemic norms reported a lower level of COVID-19 risk perception, which in turn would be associated with fewer negative emotions, lower pressure, more positive emotions, higher levels of trusts, and more confidence in fighting against COVID-19. Our findings show that perceived tighter social norms are linked to beneficial psychological outcomes. This research helps governments, institutions, and individuals understand the mechanism and benefits of social norms during the pandemic, thereby facilitating policy formulation and better responses to social crises.
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http://dx.doi.org/10.1186/s12889-022-13744-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305059PMC
July 2022

Development and evaluation of an Ultra-Wide Bandwidth based electronic hand hygiene monitoring system.

Am J Infect Control 2022 Jul 19. Epub 2022 Jul 19.

Department of Hospital-Acquired Infection Control, Beijing Jishuitan Hospital, The Forth Medical College of Peking University, Beijing, China; Department of Burns and Plastic Surgery, Beijing Jishuitan Hospital, The Forth Medical College of Peking University, Beijing, China. Electronic address:

Background: A large variety of electronic hand hygiene monitoring systems (EHHMS) are being developed and applied in health care settings. Monitoring hand hygiene (HH) opportunity at bed-level has been the key technical challenge. Accuracy evaluation needs more attention as the prerequisite upon widespread acceptance and adoption.

Methods: For the first time, we explored, debugged and upgraded an EHHMS based on ultra-wide bandwidth (UWB) which can obtain HH opportunities at bed-level. The real-time positioning and electronic fence of UWB technology was applied for EHHMS. The accuracy of EHHMS was compared with the simultaneous manual direct observations in real-world clinical setting. Sensitivity and specificity were calculated for EHHMS capturing HH action and opportunity.

Results: Two generations of EHHMS were constructed. For the first generation, the system properly recorded 84.00% and 78% of the pre-identified HH actions and opportunities performed by experimenters. For the second generation, Sensitivity and specificity of the system capturing HH action were 89.00% (84.83-92.36) and 100.00% (98.26-100.00), respectively. For capturing HH opportunity, the system showed the sensitivity and specificity of 86.52% (82.52-89.89) and 88.10% (84.14-91.36)), respectively.

Conclusion: The EHHMS based on UWB could accurately identify HH action and opportunity with equivalent accuracy compared with simultaneous direct observation.
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http://dx.doi.org/10.1016/j.ajic.2022.07.008DOI Listing
July 2022

Risk Factors for and Outcomes of Postoperative Entrapped Temporal Horn in Trigone Meningiomas.

Neurol India 2022 May-Jun;70(3):965-971

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, People's Republic of China.

Background: Meningioma in the ventricle triangle area is a benign tumor that can be cured by surgery, but postoperative entrapped temporal horn may seriously affect the patient's quality of life and even require a second operation. Currently, there are few reports on this complication.

Objective: The risk factors and prognosis of postoperative ETH in trigone meningiomas were investigated.

Material And Methods: A retrospective analysis of the clinical data of 87 patients with trigone meningioma from 2010 to 2018 was performed, and univariate and multivariate analyses were used to assess the risk factors associated with postoperative ETH. The degree of ETH was evaluated using the modified ventriculocranial ratio.

Results: The incidence of postoperative ETH in trigone meningioma was 29.9% (26/87). Preoperative ETH [odds ratio (OR): 4.826, 95% confidence interval (CI): 1.820-12.796, P = 0.002] and postoperative meningitis (OR: 12.811, 95%CI: 1.615-101.605, P = 0.016) are independent risk factors for postoperative ETH. Of the 18 patients with ETH syndrome, 12 improved after medical treatment, and finally, a total of 6 patients received ETH surgery. The mean duration from tumor resection to the appearance of ETH syndrome was 3.1 ± 1.9 months (range: 9 days-7 months). Patients with ETH grade II and III are more prone to clinical symptoms.

Conclusions: : The incidence of postoperative ETH for trigone meningiomas is high, and clinical symptoms generally appear delayed after surgery. Reducing postoperative infections can reduce the occurrence of postoperative ETH. Patients with symptoms of ETH who have failed medical treatment have clear indications for surgery.
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http://dx.doi.org/10.4103/0028-3886.349637DOI Listing
July 2022

Dexamethasone Sensitizes Acute Monocytic Leukemia Cells to Ara-C by Upregulating FKBP51.

Front Oncol 2022 4;12:888695. Epub 2022 Jul 4.

Department of Central Laboratory, Shandong Provincial Hospital, Shandong University, Jinan, China.

In this study, we demonstrated that the expression of FK506 binding protein 51 (FKBP51) is upregulated in acute monocytic leukemia (AML-M5) cells by dexamethasone and aimed to investigate the possible effects of FKBP51 on the growth and cytarabine sensitivity of AML-M5 cells. THP-1 and U937cells were used to establish AML-M5 cell models with FKBP51 overexpression and knockdown, respectively. Cell proliferation, apoptosis and response to cytarabine were investigated by cell cycle, CCK-8 and Flow cytometry analyses. The mice experiment was conducted to detect the role of FKBP51 on AML-M5 cells proliferation and antileukemia effect of Ara-C/Dexamethasone co-therapy . Western blots were employed to determine protein expression levels. FKBP51 upregulation significantly attenuated THP-1 cell proliferation and sensitized the cells to cytarabine treatment which was further enhanced by dexamethasone. These effects were indicated by decreases in cell viability, S-G2/M phase cell cycle distribution, cytarabine 50% inhibitory concentration (IC50) values and increases in apoptosis and were supported by decreased phosphorylation levels of AKT, GSK3β and FOXO1A and decreased levels of BCL-2 and increased levels of P21 and P27. In contrast, FKBP51 knockdown led to excessive U937 cell proliferation and cytarabine resistance, as indicated by increased cell viability and S-G2/M phase cell cycle distribution, decreased apoptosis, increased phosphorylation levels of AKT, GSK3β and FOXO1A, and increased BCL-2 and decreased P21 and P27 expression. In addition, an AKT inhibitor blocked cell cycle progression and reduced cell viability in all groups of cells. Furthermore, SAFit2, a specific FKBP51 inhibitor, increased U937 cell viability and cytarabine resistance as well as AKT phosphorylation. In conclusion, FKBP51 decelerates proliferation and improves the cytarabine sensitivity of AML-M5 cells by inhibiting AKT pathways, and dexamethasone in combination with Ara-C improves the chemosensitivity of AML-M5.
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http://dx.doi.org/10.3389/fonc.2022.888695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290766PMC
July 2022

[Rationality evaluation of Shuanghuanglian Injection combined with Ampicillin Sodium for Injection based on sequential analysis].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(12):3380-3385

Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University Beijing 100050, China Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use Beijing 100038, China.

The lack of rationality evaluation method for drug combination has long restricted its clinical application. In view of this, this study took Shuanghuanglian Injection as model drug and established a "physical-chemical-biological" sequential analysis method, which is expected to provide clues for improving the safety and effectiveness of clinical drug combination. With the methods of insoluble particle testing, isothermal titration calorimetry(ITC), and real time cellular analysis(RTCA), the rationality of Shuanghuanglian Injection combined with Ampicillin Sodium for Injection was assessed. The results showed that the number of insoluble particles>10 μm in the solution of the combination met the standard of Chinese Pharmacopoeia, while the number of insoluble particles>25 μm did not meet the standard. ITC detection demonstrated that the change of Gibbs free energy(ΔG) was less than 0 during the fusion process, indicating that the process was spontaneous and enthalpy-driven reaction. Therefore, the interaction between the two was mainly chemical reaction, and the internal substances may change. RTCA found that Shuanghuanglian Injection alone and Ampicillin Sodium for Injection alone basically had no inhibitory effect on the growth of HEK293 T cells, while the combination of the two suppressed the growth of HEK293 T cells, suggesting that the combination was toxic to HEK293 T cells. This study showed that Shuanghuanglian Injection and Ampicillin Sodium for Injection reacted, yielding toxicity. This suggested that the two should not be combined for application. With the "physical-chemical-biological" sequential analysis, the molecular interaction of drugs was clarified. The method can be further applied for evaluating the rationality of other Chinese and western medicine injections.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211201.701DOI Listing
June 2022

Glycine ameliorates MBP-induced meiotic abnormalities and apoptosis by regulating mitochondrial-endoplasmic reticulum interactions in porcine oocytes.

Environ Pollut 2022 Jul 12;309:119756. Epub 2022 Jul 12.

Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, China. Electronic address:

Monobutyl phthalate (MBP) is the main metabolite of dibutyl phthalate (DBP) in vivo. MBP has a stable structure, can continuously accumulate in living organisms, and has the potentially to harm animal and human reproductive function. In the ovarian follicle microenvironment, MBP may lead to defects in follicular development and steroid production, abnormal meiotic maturation, impaired ovarian function and other reproductive deficits. In this study, SMART-seq was used to investigate the effects of MBP exposure on the in vitro maturation (IVM) and development of porcine oocytes. The results showed that differentially expressed genes after MBP exposure were enriched in the biological processes cytoskeleton, cell apoptosis, endoplasmic reticulum (ER) and mitochondria. Glycine (Gly) improved the developmental potential of porcine oocytes by regulating mitochondrial and ER function. The effect of Gly in protecting oocytes against MBP-induced damage was studied. The results showed that the addition of Gly significantly decreased the rate of MBP-induced spindle abnormalities, decreased the frequency of MBP-induced mitochondria-associated ER membrane (MAM) interactions, and downregulated the protein and gene expression of the linkage molecules Mitofusin 1 (MFN1) and Mitofusin 2 (MFN2) in the MAM. Additionally, treatment with Gly restored the distribution of the 1,4,5-triphosphate receptor 1 (IPR1) and voltage-dependent anion channel 1 (VDAC1), further decreasing the intracellular free calcium concentration ([Ca]) levels and mitochondrial Ca ([Ca]) , increasing the ER Ca ([Ca]) levels, and thus significantly increasing the ER levels and mitochondrial membrane potential (ΔΨ m). Gly also decreased the levels of reactive oxygen species (ROS) and increased the levels of Glutathione (GSH), oocyte apoptosis-related indicators (Caspase-3 activity and Annexin V) and oocyte apoptosis-related genes (BAX, Caspase 3 and AIFM1). Our results suggest that Gly can ameliorate microtubule cytoskeleton abnormalities and improve oocyte maturation by reducing the defective mitochondrial-ER interactions caused by MBP exposure in vitro.
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http://dx.doi.org/10.1016/j.envpol.2022.119756DOI Listing
July 2022

An event-related potential study of self-positivity bias in native and foreign language contexts.

Psychophysiology 2022 Jul 14:e14145. Epub 2022 Jul 14.

Research Center of Brain and Cognitive Neuroscience, Liaoning Normal University, Dalian, China.

Self-positivity bias is a common psychological phenomenon in which individuals often associate positive information with themselves. However, little is known about how self-positivity bias is modulated by different language contexts (e.g., a first vs. second language). To this end, we analyzed behavioral and electrophysiological data to examine whether first or second languages play differential roles in the self-positivity bias effect. We used a modified self-positivity bias task which required Chinese-English bilinguals to judge strings of letters or characters as realwords or not and match associations between identity (self, other) and a geometric shape (circle, triangle). The target words in the experiment consisted of positive, negative, and neutral emotional words. The results showed that in the L2 context, the self-positivity condition elicited a smaller N400 effect relative to the self-negativity condition and a larger late positive component effect relative to the self-negativity and self-neutrality conditions. Furthermore, the other-positivity condition elicited a stronger N400 effect than the other-neutrality condition. These patterns did not emerge in the L1 context. We discuss the implications and contributions of these findings to better understand the interaction between emotion and self-concept in different language contexts.
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http://dx.doi.org/10.1111/psyp.14145DOI Listing
July 2022

Template-independent genome editing in the Pcdh15 mouse, a model of human DFNB23 nonsyndromic deafness.

Cell Rep 2022 Jul;40(2):111061

School of Life Sciences, Tsinghua University, Beijing 100084, China; IDG/McGovern Institute for Brain Research at Tsinghua University, Tsinghua University, Beijing 100084, China. Electronic address:

Although frameshift mutations lead to 22% of inherited Mendelian disorders in humans, there is no efficient in vivo gene therapy strategy available to date, particularly in nondividing cells. Here, we show that nonhomologous end-joining (NHEJ)-mediated nonrandom editing profiles compensate the frameshift mutation in the Pcdh15 gene and restore the lost mechanotransduction function in postmitotic hair cells of Pcdh15 mice, an animal model of human nonsyndromic deafness DFNB23. Identified by an ex vivo evaluation system in cultured cochlear explants, the selected guide RNA restores reading frame in approximately 50% of indel products and recovers mechanotransduction in more than 70% of targeted hair cells. In vivo treatment shows that half of the animals gain improvements in auditory responses, and balance function is restored in the majority of injected mutant mice. These results demonstrate that NHEJ-mediated reading-frame restoration is a simple and efficient strategy in postmitotic systems.
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http://dx.doi.org/10.1016/j.celrep.2022.111061DOI Listing
July 2022

Mutation update of SERPING1 related to hereditary angioedema in the Chinese population.

Hereditas 2022 Jul 11;159(1):28. Epub 2022 Jul 11.

Department of Allergy & Clinical Immunology, National Clinical Research Center for Immunologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, #1 Shuaifuyuan, Wangfujing, Beijing, 100730, P.R. China.

Background: Hereditary angioedema (HAE) is a rare disease characterized by recurrent attacks of severe swellings of the skin and submucosa. More than 900 variants of the SERPING1 gene associated with HAE have been identified. However, only approximately 50 variants have been identified in the Chinese population. This study aimed to update the mutational spectrum in Chinese HAE patients and provide evidence for the accurate diagnosis of HAE.

Methods: A total of 97 unrelated HAE patients were enrolled in the study. Sanger sequencing and multiple ligation-dependent probe amplification analysis were used to identify the variants in the SERPING1 gene. The variants were reviewed in a number of databases, including the Human Gene Mutation Database (HGMD) ( http://www.hgmd.cf.ac.uk/ ) and the Leiden Open Variation Database (LOVD, https://databases.lovd.nl/shared/variants/SERPING1 ). The American College of Medical Genetics and Genomics-Association for Molecular Pathology (ACMG-AMP) criteria was used to determine the pathogenicity of the variants.

Results: Of the 97 patients, 76 different variants were identified in 90 of them and no disease-causing variants were identified in the remaining 7 patients. Among the 76 variants, 35 variants were novel and submitted to ClinVar. Missense and in-frame variants were the most common variants (36.8%), followed by frameshift (28.9%), nonsense (14.5%), splice site (13.2%) variants, and gross deletions/duplications (6.6%).

Conclusions: Our findings broaden the mutational spectrum of SERPING1 and provide evidence for accurate diagnosis and predictive genetic counseling.
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http://dx.doi.org/10.1186/s41065-022-00242-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277798PMC
July 2022

ddRAD-Seq reveals evolutionary insights into population differentiation and the cryptic phylogeography of in Mainland China.

Ecol Evol 2022 Jul 4;12(7):e9053. Epub 2022 Jul 4.

Guangdong Province Key Laboratory for Aquatic Economic Animals State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) Guangzhou China.

Species differentiation and local adaptation in heterogeneous environments have attracted much attention, although little is known about the mechanisms involved. is an anadromous, brackish-water halfbeak that is widely distributed in coastal areas and hyperdiverse freshwater systems in China, making it an interesting model for research on phylogeography and local adaptation. Here, 156 individuals were sampled at eight sites from heterogeneous aquatic habitats to examine environmental and genetic contributions to phenotypic divergence. Using double-digest restriction-site-associated DNA sequencing (ddRAD-Seq) in the specimens from the different watersheds, 5498 single nucleotide polymorphisms (SNPs) were found among populations, with obvious population differentiation. We find that present-day Mainland China populations are structured into distinct genetic clusters stretching from southern and northern ancestries, mirroring geography. Following a transplant event in Plateau Lakes, there were virtually no variations of genetic diversity occurred in two populations, despite the fact two main splits were unveiled in the demographic history. Additionally, dorsal, and anal fin traits varied widely between the southern group and the others, which highlighted previously unrecognized lineages. We then explore genotype-phenotype-environment associations and predict candidate loci. Subgroup ranges appeared to correspond to geographic regions with heterogeneous hydrological factors, indicating that these features are likely important drivers of diversification. Accordingly, we conclude that genetic and phenotypic polymorphism and a moderate amount of genetic differentiation occurred, which might be ascribed to population subdivision, and the impact of abiotic factors.
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http://dx.doi.org/10.1002/ece3.9053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251877PMC
July 2022

Transcranial Direct Current Stimulation Over the Left Dorsolateral Prefrontal Cortex Reduced Attention Bias Toward Negative Facial Expression: A Pilot Study in Healthy Subjects.

Front Neurosci 2022 20;16:894798. Epub 2022 Jun 20.

Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China.

Research in the cognitive neuroscience field has shown that individuals with a stronger attention bias for negative information had higher depression risk, which may be the underlying pathogenesis of depression. This dysfunction of affect-biased attention also represents a decline in emotion regulation ability. Clinical studies have suggested that transcranial direct current stimulation (tDCS) treatment can improve the symptoms of depression, yet the neural mechanism behind this improvement is still veiled. This study aims to investigate the effects of tDCS on affect-biased attention. A sample of healthy participants received 20 min active ( = 22) or sham tDCS ( = 19) over the left dorsolateral prefrontal cortex (DLPFC) for 7 consecutive days. Electroencephalographic (EEG) signals were recorded while performing the rest task and emotional oddball task. The oddball task required response to pictures of the target (positive or negative) emotional facial stimuli and neglecting distracter (negative or positive) or standard (neutral) stimuli. Welch power spectrum estimation algorithm was applied to calculate frontal alpha asymmetry (FAA) in the rest task, and the overlapping averaging method was used to extract event-related potentials (ERP) components in the oddball task. Compared to sham tDCS, active tDCS caused an obvious increment in FAA in connection with emotion regulation ( < 0.05). Also, participants in the active tDCS group show greater P3 amplitudes following positive targets ( < 0.05) and greater N2 amplitudes following negative distracters ( < 0.05), reflecting emotion-related attention biases. These results offer valuable insights into the relationship between affect-biased attention and the effects of tDCS, which may be of assistance in exploring the neuropathological mechanism of depression and anxiety and new treatment strategies for tDCS.
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http://dx.doi.org/10.3389/fnins.2022.894798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256464PMC
June 2022

[SAPHO Syndrome Complicated with Ulcerative Colitis:Report of One Case].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2022 Jun;44(3):535-539

Department of Gastroenterology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.

Synovitis,acne,pustulosis,hyperostosis,and osteitis (SAPHO) syndrome is a rare disease. The previous literature demonstrated that about 10% of the patients with SAPHO syndrome were complicated with inflammatory bowel disease.So far,few cases of SAPHO syndrome complicated with inflammatory bowel disease have been reported in China.Herein,we reported a SAPHO syndrome case complicated with ulcerative colitis. The patient suffered from recurrent attacks of colitis following treatment of SAPHO syndrome with etanercept.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13913DOI Listing
June 2022
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