Publications by authors named "Shuang Liang"

616 Publications

Iron Overload-Induced Ferroptosis Impairs Porcine Oocyte Maturation and Subsequent Embryonic Developmental Competence .

Front Cell Dev Biol 2021 28;9:673291. Epub 2021 May 28.

Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.

Accumulating evidence indicates that ferroptosis is an iron-dependent form of regulated cell death. This type of iron-dependent programmed cell death is different from traditional forms of regulated cell death, such as apoptosis and autophagy. However, the role of ferroptosis in porcine oocyte maturation and the associated mechanism remain unclear. In the present research, we investigated the effects of ferric ammonium citrate (FAC), a specific ferroptosis inducer, on porcine oocyte meiotic maturation and quality and subsequent embryonic developmental competence. FAC treatment caused obvious accumulation of intracellular ferrous ions in porcine oocytes. At the end of the maturation (IVM) period, there was a significant decrease in the polar body (PB) extrusion rate and an increase in the percentage of abnormal oocytes in the FAC treatment groups, indicating that iron overload-induced ferroptosis may suppress the meiotic process during porcine oocyte maturation. We also found that after FAC treatment, the subsequent two-cell rate, four-cell rate and blastocyst formation rate were significantly decreased in porcine parthenogenetic activation (PA) embryos, indicating that iron overload-induced ferroptosis decreased porcine oocyte quality. Further analysis revealed that FAC treatment not only enhanced intracellular reactive oxygen species (ROS) generation, decreased intracellular free thiol levels and induced mitochondrial dysfunction but also triggered autophagy in porcine oocytes. Taken together, these findings suggest that iron overload-induced ferroptosis impairs porcine oocyte meiosis and decreases porcine oocyte quality, possibly by increasing oxidative stress, inducing mitochondrial dysfunction and triggering autophagy.
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http://dx.doi.org/10.3389/fcell.2021.673291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194094PMC
May 2021

Optimization of nutrient removal performance of magnesia-containing constructed wetlands: a microcosm study.

Environ Sci Pollut Res Int 2021 Jun 12. Epub 2021 Jun 12.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, Shandong, China.

Recently, magnesia has drawn much attention for enhancing phosphorus (P) removal of constructed wetlands. However, the poor nitrogen (N) removal efficiency of magnesia-containing constructed wetlands (Mg-CWs) inherently caused by magnesia impedes its application. In this study, peat and intermittent aeration were applied to enhance N removal in a Mg-CW, identified as P-CW and A-CW, respectively. A high TP removal rate (around 90%) was achieved in all CW, and the TN removal rate in the P-CW was 91.05% higher than that in the Mg-CW, which was mainly because the carbon source provided by the peat directly promoted the growth and metabolism of microorganisms and plants. Higher fresh weight of plants was obtained in P-CW (64.94 ± 5.78 g), compared with A-CW (35.88 ± 15.25 g) and Mg-CW (46.25 ± 18.88 g), accomplished by stronger tolerance to high pH (>10). The microbial abundance (16S rRNA) in the P-CW was 15.6 and 8.12 times higher than that of Mg-CW and A-CW, respectively, resulting in lower global warming potential. Tanking all factors into consideration, addition of peat could be an effective method to optimize the nutrient removal performance of Mg-CW.
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http://dx.doi.org/10.1007/s11356-021-14785-7DOI Listing
June 2021

Non-degradable collagen increases liver fibrosis but not hepatocellular carcinoma in mice.

Am J Pathol 2021 Jun 10. Epub 2021 Jun 10.

Departments of Medicine, UC San Diego, San Diego, CA. Electronic address:

Although hepatocellular cancer (HCC) usually occurs in the setting of liver fibrosis, the causal relationship between liver fibrosis and HCC is unclear. By studying in vivo and in vitro models of HCC using Col mice (that produce a collagenase resistant Type I collagen) or WT mice, we aim to assess the relationship between Type I collagen, liver fibrosis, and experimental HCC. HCC was either chemically induced in WT and Col mice or Hepa 1-6 cells were engrafted into WT and Col livers. The effect of Hepatic Stellate Cells (HSCs) from WT and Col mice on the growth of Hepa 1-6 cells was studied by using multicellular tumor spheroids (MCTS) and by xenografts. Although Collagen Type I deposition and fibrosis was increased in Col mice, they developed less and smaller tumors. Hepa 1-6 cells had reduced tumor growth in the livers of Col mice. Although Col HSCs exhibited a more activated phenotype, Hepa 1-6 growth and malignancy were suppressed in MCTS and in xenografts containing Col HSCs. Treatment with vitronectin, which mimics the presence of degraded collagen fragments, converted the Col phenotype into WT phenotype. In conclusion, although Col mice have increased liver fibrosis, they show decreased HCC in several models. Thus, increased liver type I collagen does not produce increased experimental HCC.
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http://dx.doi.org/10.1016/j.ajpath.2021.05.019DOI Listing
June 2021

Recent Advances in Synthesis, Properties, and Applications of Metal Halide Perovskite Nanocrystals/Polymer Nanocomposites.

Adv Mater 2021 Jun 6:e2005888. Epub 2021 Jun 6.

School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.

Metal halide perovskite nanocrystals (PNCs) have recently garnered tremendous research interest due to their unique optoelectronic properties and promising applications in photovoltaics and optoelectronics. Metal halide PNCs can be combined with polymers to create nanocomposites that carry an array of advantageous characteristics. The polymer matrix can bestow stability, stretchability, and solution-processability while the PNCs maintain their size-, shape- and composition-dependent optoelectronic properties. As such, these nanocomposites possess great promise for next-generation displays, lighting, sensing, biomedical technologies, and energy conversion. The recent advances in metal halide PNC/polymer nanocomposites are summarized here. First, a variety of synthetic strategies for crafting PNC/polymer nanocomposites are discussed. Second, their array of intriguing properties is examined. Third, the broad range of applications of PNC/polymer nanocomposites is highlighted, including light-emitting diodes (LEDs), lasers, and scintillators. Finally, an outlook on future research directions and challenges in this rapidly evolving field are presented.
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http://dx.doi.org/10.1002/adma.202005888DOI Listing
June 2021

rs1344706 polymorphism of zinc finger protein 804a (ZNF804a) gene related to the integrity of white matter fiber bundle in schizophrenics.

Exp Ther Med 2021 Jul 18;22(1):778. Epub 2021 May 18.

Department of Psychiatry, Qiqihar Medical University, Qiqihar, Heilongjiang 161000, P.R. China.

Genetic factors play an important role in the pathogenesis of schizophrenia (SZ), and the zinc finger protein 804a (ZNF804a) gene has been considered to be a risk gene for schizophrenia. In the present study, the correlation between rs1344706 polymorphism of ZNF804a gene and the integrity of white matter in schizophrenic cases was explored. A total of 60 SZ patients and 100 healthy controls (HC) were included to undergo head MRI. According to the genotyping of rs1344706 in ZNF804a, the subjects in each group were divided into a normal allele and risk allele-carrying group. The imaging data were preprocessed by PANDA software, and thefractional anisotropy (FA) of each subject was calculated. With SPM8 software, age and years of education were considered as covariates, and diagnosis as well as genotype (AA, GG/AG) were considered as intergroup factors. Four groups of FA images were analyzed by two-factor analysis of variance. The FA value of the right posterior radiocrown in the patient group was lower than that in the control group, and the difference was statistically significant. The FA value of the right lower frontal occipital tract and the right upper radiocrown in the G allele carrier group was lower than that in the A allele homozygous group. There was detection of an interaction between the FA value of the splenium of corpus callosum, the body part of the corpus callosum and the right cingulate tract. In the present study, it was demonstrated that the rs1344706 GG/AG genotype of the ZNF804a gene locus in SZ patients suffered from abnormal structure in a specific region of the brain. This finding indicated that the rs1344706 single nucleotide polymorphism of the ZNF804a gene may affect the integrity of the white matter of the brain in SZ patients and may be involved in the pathophysiological mechanism of SZ.
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http://dx.doi.org/10.3892/etm.2021.10210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145689PMC
July 2021

Celecoxib reduces inflammation and angiogenesis in mice with adenomyosis.

Am J Transl Res 2021 15;13(4):2858-2866. Epub 2021 Apr 15.

Department of Gynecology, The Third Affiliated Hospital of Zhengzhou University China.

Objective: This study aimed to explore the effect of COX-2 selective inhibitor (celecoxib) on adenomyosis and its mechanism.

Methods: By establishing a mouse model of adenomyosis and using celecoxib to treat adenomyosis, newly born female mice were randomly divided into a control group, adenomyosis model group, and celecoxib group. Hematoxylin-eosin (H&E) staining was used to observe the depth of endometrial infiltration of mouse adenomyosis. RT-PCR (reverse transcription PCR) and western blot were used to detect the expression of Cyclooxygenase-2 (COX-2), Vascular growth factor (VEGF), Nerve growth factor (NGF), and Corticotropin-releasing hormone (CRH) mRNA and protein in mice before and after celecoxib treatment.

Results: After treatment with celecoxib, the depth of endometrial infiltration of mouse adenomyosis was reduced. COX-2 and VEGF decreased significantly after celecoxib inhibited expression of COX-2 (P<0.001), but there was no significant difference in the expression of NGF or CRH (P>0.05).

Conclusion: This study indicated that COX-2 may be an important factor related to the pathogenesis of adenomyosis, and it may become an important molecular target for the treatment of adenomyosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129283PMC
April 2021

Up-regulation of MARVEL domain-containing protein 1 (MARVELD1) accelerated the malignant phenotype of glioma cancer cells via mediating JAK/STAT signaling pathway.

Braz J Med Biol Res 2021 17;54(7):e10236. Epub 2021 May 17.

Department of Operating Room, Hongqi Hospital Affiliated to Mudanjiang Medical College, Mudanjiang, China.

This work aimed to research the function of MARVEL domain-containing protein 1 (MARVELD1) in glioma as well as its functioning mode. Bioinformatics analysis was utilized to assess the MARVELD1 expression in glioma tissues and its relationship with grade and prognosis, based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Chinese Glioma Genome Atlas (CGGA) databases. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays were carried out to determine the impact of MARVELD1 on malignant biological behavior of glioma, such as proliferation, invasion, and migration. qRT-PCR was carried out to test the mRNA level of MARVELD1. Western blot assay was performed to measure the protein expression of MARVELD1 and JAK/STAT pathway-related proteins. MARVELD1 was expressed at high levels in glioma tissues and cell lines. Kaplan-Meier survival analysis revealed that the higher MARVELD1 expression, the shorter the survival time of patients with glioma. Also, the MARVELD1 expression in WHO IV was significantly enhanced compared to that in WHO II and WHO III. Furthermore, the functional analysis of MARVELD1 in vitro revealed that knockdown of MARVELD1 in U251 cells restrained cell proliferation, migration, and invasion, while up-regulation of MARVELD1 in U87 cells presented opposite outcomes. Finally, we found that JAK/STAT signaling pathway mediated the function of MARVELD1 in glioma. MARVELD1 contributed to promoting the malignant progression of glioma, which is the key driver of activation of JAK/STAT signaling pathway in gliomas.
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http://dx.doi.org/10.1590/1414-431X2020e10236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130134PMC
May 2021

Enhanced phosphorus removal of constructed wetland through plant growth-promoting rhizobacteria (PGPR) addition.

Environ Sci Pollut Res Int 2021 May 17. Epub 2021 May 17.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, People's Republic of China.

Phosphorus (P) removal efficiency of constructed wetland (CW) was limited due to the adsorption saturation on substrate surface along with continuous operation of CW. This study attempted to improve the P removal of CW through introduction of plant growth-promoting rhizobacteria (PGPR). Compared with the control-CW (C-CW), the results of CW with bio-augmentation (B-CW) showed that the total phosphorus (TP) removal efficiency was increased by 36.7% due to the enhanced plant uptake of P. The physiology indicators (height and root activity) of plants in B-CW were significantly improved, and the average P content of plants in B-CW was 0.78 g/kg, which was 85.7% higher than that of C-CW (0.42 g/kg). This was because PGPR addition optimized the P forms adsorbed on substrate surface and increased the proportion of Ca/Mg-P which was bioavailable for plant growth, and then subsequently enhanced plant uptake of P. Through bio-augmentation, the proportion of P removal by plant uptake in B-CW (25.2%) was increased by 2.5 times compared with that of C-CW (7.1%). The outcomes of this study would shed light on intensifying the role of plant uptake in P removal of CWs through bio-augmentation.
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http://dx.doi.org/10.1007/s11356-021-14364-wDOI Listing
May 2021

Comparison of Postoperative Pain Management Outcomes in the United States and China.

Clin Nurs Res 2021 May 17:10547738211012832. Epub 2021 May 17.

Kiang Wu Nursing College of Macau, China.

To compare pain management outcomes in postoperative patients from an American hospital and a Chinese hospital. A convenience sample of 244 patients in the United States and 268 patients in China with similar surgical sites completed the American Pain Society Patient Outcome Questionnaire-Revised (APS-POQ-R) and the Pain Management Index (PMI) was calculated on their first postoperative day. Patients in the United States reported a higher score on the "perception of pain management" subscale of the APS-POQ-R and a higher proportion of adequate treatment as measured by the PMI (85.2% vs. 39.0%,  < .001). Patient education and degrees of pain relief predicted patient satisfaction with pain management (item in APS-POQ-R) in patients from both countries. A higher level of compliance with pain management guidelines has contributed to a higher level of perception in patients in the United States. Establishing procedure-specific protocols and clinical pathways may improve pain management outcomes for Chinese patients.
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http://dx.doi.org/10.1177/10547738211012832DOI Listing
May 2021

Gas-blasting nanocapsules to accelerate carboplatin lysosome release and nucleus delivery for prostate cancer treatment.

Asian J Pharm Sci 2021 Mar 20;16(2):192-202. Epub 2020 Jun 20.

Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.

To improve therapeutic effect and reduce severely side effects of carboplatin (CBP), the gas-generating nanocapsules were developed to accelerate CBP lysosome release and nucleus delivery. CBP/SB-NC was prepared by co-loading CBP and NaHCO (SB) in nanocapsules using w/o/w emulsification solvent evaporation. They exhibited vesicle-like spherical morphology, uniform particle size and negative zeta potential. Reaching the tumor site with a relatively high concentration is the first step for CBP delivery and the results showed that CBP/SB-NC could effectively increase drug accumulation at tumor site. After that, the drug delivery carriers need to be internalized into tumor cells and the cellular uptake ability results showed CBP/SB-NC could be internalized into RM-1 cells more efficient than CBP solution. After internalized by RM-1 cells, the gas-blasting release process was tested in acid environment. It was demonstrated that 5 mg/ml NaHCO was optimal to achieve pH-responsive gas-blasting release. release results showed that CBP significantly rapid release in acid environment (pH 5.0) compared to neutral pH (pH 7.4) ( < 0.05). Meanwhile, TEM and the change of the concentration of H results exhibited that the explosion of CBP/SB-NC was more easily happened in lysosome acid environment (pH 5.0). The blasting release can accelerate CBP lysosome release to cytoplasm. Furthermore, the nucleus delivery results showed CBP/SB-NC can promote pH-triggered rapid nucleus delivery. And the results of Pt-DNA adduct assay showed that the binding efficiency between CBP and DNA of CBP/SB-NC was higher than CBP solution. At last, and anti-tumor efficacy proved that CBP/SB-NC could enhance anti-tumor activity for prostate cancer therapy. CBP/SB-NC also showed superior safety and by hemolysis assay and histopathological study. All of the results demonstrate that CBP/SB-NC would be an efficient gas-blasting release formulation to enhance prostate cancer treatment.
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http://dx.doi.org/10.1016/j.ajps.2020.05.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105516PMC
March 2021

PM exposure exaggerates the risk of adverse birth outcomes in pregnant women with pre-existing hyperlipidemia: Modulation role of adipokines and lipidome.

Sci Total Environ 2021 Sep 8;787:147604. Epub 2021 May 8.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, People's Republic of China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, People's Republic of China. Electronic address:

The in-utero environmental exposure to fine particulate matter (PM) might lead to adverse birth outcomes, such as low birth weight (LBW) and preterm birth (PTB), thereby increasing susceptibility to diseases in later life. However, no studies have examined the underlying mechanism through cross-omics of lipidome and adipokines profiling, as well as the possible effect modification by maternal hyperlipidemia. In total, 203 mother-newborn pairs were recruited in the birth cohort study ongoing since February 2017 in Beijing, China. Individual-level of PM exposure was estimated using a satellite data based random forest model. Cord blood lipidome and adipokines were assessed through the lipidomic approaches and antibody-based array. Multivariable logistic/linear regression models and moderation analysis were employed in this study. We observed a significantly increased risk of PTB associated with PM exposure during the second trimester, especially in pregnant women with pre-existing hyperlipidemia. 9 lipid classes and 21 adipokines were associated with PM exposure independently or significantly influenced by the interaction of maternal PM exposure and hyperlipidemia. In addition, 4 adipokines (ANGPTL4, IGFBP-2, IL-12p40, and TNF-RII) and 3 lipid classes [phosphatidylcholines (PCs), phosphatidylinositols (PIs), and triglycerides (TGs)] were related to the increased risk of PTB, indicating that inflammation, IGF/IGFBP axis, and lipolysis induced lipid homeostasis disorder of PCs, TGs, and PIs might be the possible mediators for the PM-induced adverse birth outcomes. Our results substantiated the need for reducing exposure in susceptible populations.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147604DOI Listing
September 2021

Porphyromonas gingivalis infection exacerbates oesophageal cancer and promotes resistance to neoadjuvant chemotherapy.

Br J Cancer 2021 May 12. Epub 2021 May 12.

VCU Philips Institute for Oral Health Research, Department of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University School of Dentistry, Richmond, VA, USA.

Background: The effect of Porphyromonas gingivalis (Pg) infection on oesophageal squamous cell carcinoma (ESCC) prognosis, chemotherapeutic efficacy, and oesophageal cancer cell apoptosis resistance and proliferation remain poorly understood.

Methods: Clinicopathological data from 312 ESCC oesophagectomy patients, along with the computed tomography imaging results and longitudinal cancerous tissue samples from a patient subset (n = 85) who received neoadjuvant chemotherapy (NACT), were analysed. Comparison of overall survival and response rate to NACT between Pg-infected and Pg-uninfected patients was made by multivariate Cox analysis and Response Evaluation Criteria in Solid Tumours v.1.1 criteria. The influence of Pg on cell proliferation and drug-induced apoptosis was examined in ESCC patients and validated in vitro and in vivo.

Results: The 5-year overall survival was lower in Pg-positive patients, and infection was associated with multiple clinicopathological factors and pathologic tumour, node, metastasis stage. Of the 85 patients who received NACT, Pg infection was associated with a lower response rate and 5-year overall survival. Infection with Pg resulted in apoptosis resistance in ESCC and promoted ESCC cell viability, which was confirmed in longitudinal cancerous tissue samples. Pg-induced apoptosis resistance was dependent on fimbriae and STAT3.

Conclusions: Pg infection is associated with a worse ESCC prognosis, reduced chemotherapy efficacy, and can potentiate the aggressive behaviour of ESCC cells.
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http://dx.doi.org/10.1038/s41416-021-01419-5DOI Listing
May 2021

Depletion of MRPL35 inhibits gastric carcinoma cell proliferation by regulating downstream signaling proteins.

World J Gastroenterol 2021 Apr;27(16):1785-1804

Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China.

Background: Gastric carcinoma (GC) is a digestive system disease with high morbidity and mortality. However, early clinical detection is difficult, and the therapeutic effect for advanced disease is not satisfactory. Thus, finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative. MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein. MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer, which promotes the exploration of the correlation between MRPL35 and GC. We proposed that the expression of MRPL35 might be critical in GC.

Aim: To study the effect of knockdown on GC cell proliferation.

Methods: The expression of in GC was evaluated based on data from the public tumor database UALCAN (www.ualcan.path.uab.edu). The effect of the expression of on the prognosis was evaluated with KMplot (www.kmplot.com). The expression of MRPL35 was assessed on the tissue microarray by immunohistochemistry and the level of mRNA in 25 pairs of clinical GC tissues and matched adjacent tissues was detected by quantitative reverse transcription-polymerase chain reaction. Celigo cell count assay, colony formation assay, and flow cytometry were used to assess the role of MRPL35 in GC cell proliferation and apoptosis . Additionally, tumor formation experiment in BALB/c nude mice was utilized to determine the effect of on GC cell proliferation. After knockdown of , related proteins were identified by isobaric tags for relative and absolute quantification analysis, and the expression of related proteins was detected by Western blot.

Results: The expression of MRPL35 was up-regulated in GC ( = 1.77 × 10). The Kaplan-Meier plots of the overall survival indicated that high expression of MRPL35 was associated with a poor survival in GC. Compared with adjacent tissues, the expression of MRPL35 in GC tissues was increased, which was related to age ( = 0.03), lymph node metastasis ( = 0.007), and pathological tumor-node-metastasis stage ( = 0.024). Knockdown of inhibited GC cell proliferation and colony formation and induced apoptosis. Animal experiment results showed that knockdown of inhibited tumor formation in BALB/c nude mice. Western blotting analysis showed that after knockdown of , the expression of PICK1 and BCL-XL proteins decreased, and that of AGR2 protein increased.

Conclusion: Collectively, our findings demonstrate that knockdown of inhibits GC cell proliferation through related proteins including PICK1, BCL-XL, and AGR2.
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http://dx.doi.org/10.3748/wjg.v27.i16.1785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072187PMC
April 2021

Lymph Node Delivery Strategy Enables the Activation of Cytotoxic T Lymphocytes and Natural Killer Cells to Augment Cancer Immunotherapy.

ACS Appl Mater Interfaces 2021 May 6;13(19):22213-22224. Epub 2021 May 6.

Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong 250012, China.

Lymph nodes are the main sites for immune activation and surveillance. Effective delivery of immunomodulators into lymph nodes to trigger antitumor immunity is essential for cancer treatment. Here, we propose a lymph node delivery strategy to modulate the immune response by activating cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells simultaneously. Novel pH/redox dual-sensitive micelles were prepared using poly(l-histidine)-poly(ethylene glycol) (PLH-PEG) as a skeleton, which can effectively deliver immunomodulators to the lymph nodes due to their suitable particle size. At 48 h after subcutaneous injection, the accumulation efficiency in lymph nodes increased 8.12-fold compared with the control group. Subsequently, Trp2/CpG-coloaded pH/redox dual-sensitive micelles (Trp2/CpG-NPs) acted on antigen-presenting cells, fully promoting CTL activation through dendritic cell antigen cross-presentation and macrophage repolarization. IL-15-loaded pH/redox dual-sensitive micelles (IL-15-NPs) were developed to activate the killing effect of NK cells by interacting with IL-15 receptors. In the tumor-bearing mice model, this lymph node delivery strategy showed significant antitumor efficiency and the tumor inhibition rate reached 93.76%. Meanwhile, the infiltration of CTLs and NK cells in tumor tissues increased, and the immunosuppressive microenvironment was relieved by the repolarization of macrophages from M2-type to M1-type. Overall, this study highlighted the potential of the lymph node delivery strategy for cancer immunotherapy.
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http://dx.doi.org/10.1021/acsami.1c03709DOI Listing
May 2021

MiR-939-5p suppresses PM-induced endothelial injury targeting HIF-1α in HAECs.

Nanotoxicology 2021 May 3:1-15. Epub 2021 May 3.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China.

Ambient air pollution is a leading cause of non-communicable disease in the world. PM has the potential to change the miRNAs profiles, which in turn causes cardiovascular effects. Hypoxia-inducible factor (HIF)-1 plays a critical role in the development of atherosclerosis. Yet, the possible role of miR-939-5p/HIF-1α in PM-induced endothelial injury remains elusive. Therefore, the study aims to investigate the effects of miR-939-5p and HIF-1α on PM-triggered endothelial injury. The results from immunofluorescence, qRT-PCR, LSCM, and western blot assays demonstrated that PM increased the levels of HIF-1α, inflammation and apoptosis in human aortic endothelial cells (HAECs). Yet, the inflammatory response and mitochondrial-mediated apoptosis pathway were effectively inhibited in HIF-1α knockdown HAECs lines. The expression of miR-939-5p was significantly down-regulated in HAECs after exposed to PM. The luciferase reporter, qRT-PCR and western blot results demonstrated that miR-939-5p could directly targeted HIF-1α. And the miR-939-5p overexpression restricted PM-triggered decreases in cell viability and increases in lactic dehydrogenase (LDH) activity, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and inflammation. In addition, miR-939-5p overexpression remarkably suppressed PM-triggered BcL-2/Bax ratio reduction and Cytochrome C, Cleaved Caspase-9 and Cleaved Caspase-3 expression increase, revealed that miR-939-5p hampered PM-induced endothelial apoptosis through mitochondrial-mediated apoptosis pathway. Our results demonstrated that PM increased the expression of HIF-1α followed by a pro-inflammatory and apoptotic response in HAECs. The protective effect of miR-939-5p on PM-triggered endothelial cell injury by negatively regulating HIF-1α. miR-939-5p might present a new therapeutic target for PM induced endothelial injury.
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http://dx.doi.org/10.1080/17435390.2021.1917716DOI Listing
May 2021

Drug Repurposing for COVID-19 Treatment by Integrating Network Pharmacology and Transcriptomics.

Pharmaceutics 2021 Apr 14;13(4). Epub 2021 Apr 14.

Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

Since coronavirus disease 2019 (COVID-19) is a serious new worldwide public health crisis with significant morbidity and mortality, effective therapeutic treatments are urgently needed. Drug repurposing is an efficient and cost-effective strategy with minimum risk for identifying novel potential treatment options by repositioning therapies that were previously approved for other clinical outcomes. Here, we used an integrated network-based pharmacologic and transcriptomic approach to screen drug candidates novel for COVID-19 treatment. Network-based proximity scores were calculated to identify the drug-disease pharmacological effect between drug-target relationship modules and COVID-19 related genes. Gene set enrichment analysis (GSEA) was then performed to determine whether drug candidates influence the expression of COVID-19 related genes and examine the sensitivity of the repurposing drug treatment to peripheral immune cell types. Moreover, we used the complementary exposure model to recommend potential synergistic drug combinations. We identified 18 individual drug candidates including nicardipine, orantinib, tipifarnib and promethazine which have not previously been proposed as possible treatments for COVID-19. Additionally, 30 synergistic drug pairs were ultimately recommended including fostamatinib plus tretinoin and orantinib plus valproic acid. Differential expression genes of most repurposing drugs were enriched significantly in B cells. The findings may potentially accelerate the discovery and establishment of an effective therapeutic treatment plan for COVID-19 patients.
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http://dx.doi.org/10.3390/pharmaceutics13040545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069812PMC
April 2021

Richer fusion network for breast cancer classification based on multimodal data.

BMC Med Inform Decis Mak 2021 04 22;21(Suppl 1):134. Epub 2021 Apr 22.

Department of Oncology, Peking University International Hospital, Beijing, China.

Background: Deep learning algorithms significantly improve the accuracy of pathological image classification, but the accuracy of breast cancer classification using only single-mode pathological images still cannot meet the needs of clinical practice. Inspired by the real scenario of pathologists reading pathological images for diagnosis, we integrate pathological images and structured data extracted from clinical electronic medical record (EMR) to further improve the accuracy of breast cancer classification.

Methods: In this paper, we propose a new richer fusion network for the classification of benign and malignant breast cancer based on multimodal data. To make pathological image can be integrated more sufficient with structured EMR data, we proposed a method to extract richer multilevel feature representation of the pathological image from multiple convolutional layers. Meanwhile, to minimize the information loss for each modality before data fusion, we use the denoising autoencoder as a way to increase the low-dimensional structured EMR data to high-dimensional, instead of reducing the high-dimensional image data to low-dimensional before data fusion. In addition, denoising autoencoder naturally generalizes our method to make the accurate prediction with partially missing structured EMR data.

Results: The experimental results show that the proposed method is superior to the most advanced method in terms of the average classification accuracy (92.9%). In addition, we have released a dataset containing structured data from 185 patients that were extracted from EMR and 3764 paired pathological images of breast cancer, which can be publicly downloaded from http://ear.ict.ac.cn/?page_id=1663 .

Conclusions: We utilized a new richer fusion network to integrate highly heterogeneous data to leverage the structured EMR data to improve the accuracy of pathological image classification. Therefore, the application of automatic breast cancer classification algorithms in clinical practice becomes possible. Due to the generality of the proposed fusion method, it can be straightforwardly extended to the fusion of other structured data and unstructured data.
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http://dx.doi.org/10.1186/s12911-020-01340-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061018PMC
April 2021

Downregulation of miR-588 is associated with tumor progression and unfavorable prognosis in patients with osteosarcoma.

Exp Ther Med 2021 Jun 8;21(6):592. Epub 2021 Apr 8.

Department of Orthopedics, The Second Affiliated Hospital of Mudanjiang University, Mudanjiang, Heilongjiang 157009, P.R China.

Osteosarcoma (OS) is a primary malignant tumor characterized by a high metastatic potential and poor prognosis. The dysregulation of miR-588 has been demonstrated to serve crucial roles in the progression of numerous types of cancer. The present study aimed to investigate the expression and function of miR-588 in the development of OS. To do so, clinical samples were collected and analyzed, and in vitro experiments were conducted. A total of 104 patients with OS were recruited between 2012 and 2014. The expression of miR-588 was analyzed by reverse transcription quantitative PCR. The association between miR-588 expression and the clinicopathological characteristics and survival rate of patients with OS was evaluated. Furthermore, Cell Counting Kit-8 and Transwell assays were used to evaluate the effect of miR-588 on the proliferation and the migratory and invasive abilities of various OS cell lines. The results demonstrated that miR-588 expression in OS tissues and cells was significantly lower compared with normal tissues and cells. In addition, miR-588 expression was closely associated with the Musculoskeletal Tumor Society (MSTS) staging of patients with OS. miR-588 expression and MSTS staging were therefore considered as independent indicators for the prognosis of patients with OS. In addition, miR-588 downregulation significantly stimulated the proliferation and migratory and invasive abilities of OS cells. Taken together, these findings indicated that miR-588 may serve as an independent prognostic factor and tumor suppressor in OS.
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http://dx.doi.org/10.3892/etm.2021.10024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056108PMC
June 2021

A highly stable and flexible zeolite electrolyte solid-state Li-air battery.

Nature 2021 Apr 21;592(7855):551-557. Epub 2021 Apr 21.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, P. R. China.

Solid-state lithium (Li)-air batteries are recognized as a next-generation solution for energy storage to address the safety and electrochemical stability issues that are encountered in liquid battery systems. However, conventional solid electrolytes are unsuitable for use in solid-state Li-air systems owing to their instability towards lithium metal and/or air, as well as the difficulty in constructing low-resistance interfaces. Here we present an integrated solid-state Li-air battery that contains an ultrathin, high-ion-conductive lithium-ion-exchanged zeolite X (LiX) membrane as the sole solid electrolyte. This electrolyte is integrated with cast lithium as the anode and carbon nanotubes as the cathode using an in situ assembly strategy. Owing to the intrinsic chemical stability of the zeolite, degeneration of the electrolyte from the effects of lithium or air is effectively suppressed. The battery has a capacity of 12,020 milliamp hours per gram of carbon nanotubes, and has a cycle life of 149 cycles at a current density of 500 milliamps per gram and at a capacity of 1,000 milliamp hours per gram. This cycle life is greater than those of batteries based on lithium aluminium germanium phosphate (12 cycles) and organic electrolytes (102 cycles) under the same conditions. The electrochemical performance, flexibility and stability of zeolite-based Li-air batteries confer practical applicability that could extend to other energy-storage systems, such as Li-ion, Na-air and Na-ion batteries.
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http://dx.doi.org/10.1038/s41586-021-03410-9DOI Listing
April 2021

Ibandronate sodium and zoledronate sodium in the treatment of senile osteoporosis: efficacy, impact on quality of life and cost-effectiveness analysis.

Am J Transl Res 2021 15;13(3):1764-1771. Epub 2021 Mar 15.

Department of Pharmacy, The Third Hospital of Hebei Medical University Shijiazhuang, Hebei Province, China.

Objective: To investigate the efficacy of ibandronate sodium and zoledronate sodium in the treatment of senile osteoporosis and their impact on quality of life, and to analyze the cost-effectiveness.

Methods: A retrospective study was conducted on 215 patients with senile osteoporosis, who were admitted to our hospital from January 2017 to June 2019. Among them, 115 cases treated with ibandronate sodium were set to group A and 100 cases treated with zoledronate sodium were set to group B. The clinical efficacy, bone mineral density (BMD) before and after treatment, bone metabolic markers (alkaline phosphatase (ALP), blood phosphorus (P), blood calcium ion (Ca)), quality of life, adverse reactions, cost-effectiveness indicators (length of hospitalization, cost) and complications were compared between the two groups.

Results: Total therapeutic response rate in group A was 96.52% (111/115), which was not statistically different from that of 93.00% (93/100) in group B (P=0.242). After treatment, the BMD, ALP, BGP and Ca levels of the lumbar spine L1-L4, left femoral neck and right femoral neck, as well as quality of life scores in the two groups increased (P < 0.05), while serum ALP levels decreased (P < 0.05), however, there was no statistically significant difference between the two groups (P > 0.05). The incidence of adverse reactions in group A was 3.48% (4/115), which showed no statistical significance with that of 5.00% (5/100) in group B (P=0.830). The length of hospitalization, annual treatment expense, medical insurance expense and out-of-pocket payments in group A were all lower than those in group B (P < 0.05).

Conclusion: In the treatment of senile osteoporosis, the efficacy and adverse reactions of ibandronate sodium and zoledronate sodium are similar, both of them can effectively improve the quality of life. However, the cost-effectiveness of ibandronate sodium is better than that of zoledronate sodium.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014411PMC
March 2021

Conferring Ti-Based MOFs with Defects for Enhanced Sonodynamic Cancer Therapy.

Adv Mater 2021 May 1;33(18):e2100333. Epub 2021 Apr 1.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

The development of highly efficient, multifunctional, and biocompatible sonosensitizer is still a priority for current sonodynamic therapy (SDT). Herein, a defect-rich Ti-based metal-organic framework (MOF) (D-MOF(Ti)) with greatly improved sonosensitizing effect is simply constructed and used for enhanced SDT. Compared with the commonly used sonosensitizer TiO , D-MOF(Ti) results in a superior reactive oxygen species (ROS) yield under ultrasound (US) irradiation due to its narrow bandgap, which principally improves the US-triggered electron-hole separation. Meanwhile, due to the existence of Ti ions, D-MOF(Ti) also exhibits a high level of Fenton-like activity to enable chemodynamic therapy. Particularly, US as the excitation source of SDT can simultaneously enhance the Fenton-like reaction to achieve remarkably synergistic outcomes for oncotherapy. More importantly, D-MOF(Ti) can be degraded and metabolized out of the body after completion of its therapeutic functions without off-target toxicity. Overall, this work identifies a novel Ti-familial sonosensitizer harboring great potential for synergistic sonodynamic and chemodynamic cancer therapy.
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http://dx.doi.org/10.1002/adma.202100333DOI Listing
May 2021

Comparison of Postnatal Growth Charts of Singleton Preterm and Term Infants Using World Health Organization Standards at 40-160 Weeks Postmenstrual Age: A Chinese Single-Center Retrospective Cohort Study.

Front Pediatr 2021 15;9:595882. Epub 2021 Mar 15.

Department of Developmental Pediatrics and Child Health Care, The First Affiliated Hospital of Shandong First Medical University, Shandong Provincial Qianfoshan Hospital, Shandong Engineering and Technology Research Center for Pediatric Drug Development, Jinan, China.

There remains controversy regarding whether the growth charts constructed from data of term infants, such as those produced by the World Health Organization (WHO) standards, can appropriately evaluate the postnatal growth of preterm infants. This retrospective cohort study, conducted in the First Affiliated Hospital of Shandong First Medical University in Jinan China, aimed to compare the postnatal growth charts of singleton preterm and term infants using WHO standards at 40-160 weeks postmenstrual age (PMA). A total of 5,459 and 15,185 sets of longitudinal measurements [length/height, weight, head circumference (HC), and body mass index (BMI)] from birth to 160 weeks PMA were used to construct growth charts for 559 singleton preterm (mean PMA at birth, 33.84 weeks) and 1,596 singleton term infants (born at 40 weeks PMA), respectively, using the Generalized Additive Models for Location, Scale, and Shape (GAMLSS) method. Z-scores (prematurity corrected) were calculated using WHO Anthro software. Compared to WHO standards, all parameters of preterm infants were increased, especially in terms of length/height and weight; the gap between the two almost spanned two adjacent centile curves. Compared to term controls, the length/height, weight, and BMI of preterm infants were higher at 40 weeks PMA, surpassed by term infants at 52-64 weeks PMA, and quite consistent thereafter. The HC of preterm infants at 40-160 weeks PMA was quite consistent with both term controls and the WHO standards. The Z-scores for length/height, weight, and BMI of preterm infants relative to the WHO standards gradually decreased from 1.20, 1.13, and 0.74 at 40-44 weeks PMA to 0.67, 0.42, and 0.03 at 132-160 weeks PMA, respectively; Z-scores for HC of preterm infants rapidly decreased from 0.73 to 0.29 at 40-50 weeks PMA, and then fluctuated in the range of 0.08-0.23 at 50-160 weeks PMA. Preterm infants had higher growth trajectories than the WHO standards and similar but not identical trajectories to term infants during the first 2 years of life. These findings reemphasize the necessity of constructing local growth charts for Chinese singleton preterm infants.
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http://dx.doi.org/10.3389/fped.2021.595882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005644PMC
March 2021

Anti-rheumatoid arthritis potential of diterpenoid fraction derived from Rhododendron molle fruits.

Chin J Nat Med 2021 Mar;19(3):181-187

Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Rhododendron molle G. Don is first recorded in Shengnong's Herbal Classic, and its fruits, which are termed as Liuzhouzi, are often used to treat rheumatoid arthritis in Chinese folk. During our ongoing investigation to develop a safer and potential new arthritis therapy, a process for the preparation of diterpenoid fraction from Rhododendron mollefruits was established. In order to evaluate the main components and the anti-rheumatoid arthritis effect of the diterpenoid fraction, phytochemical and pharmacological experiments were used. As the result, the main components of diterpenoid fraction were identified as rhodojaponin III (1), rhodojaponin VI (2), 2-O-methylrhodojaponin (3), and 5'-β-D-glucopyranosy-loxyjasmonic acid (4). These four components constitute greater than 95% of diterpenoid fraction using area normalization method of HPLC-ELSD. The results of CIA rat experiment showed that high dose of diterpenoid fraction (0.6 mg·kg·d) significantly alleviated the symptoms of rheumatoid arthritis, similar to tripterygium polyglycosides, an effective RA therapy. Preliminary mechanism studies indicated that diterpenoid fraction significantly inhibited the abnormal proliferation of T and B lymphocytes, and remarkably reduced the levels of pro-inflammatory cytokines IL-6, IL-1β and TNF-α. Overall, our findings may provide a more effective and safe alternative treatment for RA using common clinical Chinese medicines like tripterygium polyglycosides.
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http://dx.doi.org/10.1016/S1875-5364(21)60019-5DOI Listing
March 2021

A deep convolutional neural network to simultaneously localize and recognize waste types in images.

Authors:
Shuang Liang Yu Gu

Waste Manag 2021 May 26;126:247-257. Epub 2021 Mar 26.

School of Automation, Guangdong University of Petrochemical Technology, Maoming 525000, Guangdong, China; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Department of Chemistry, Institute of Inorganic and Analytical Chemistry, Goethe-University, 60438 Frankfurt, Germany. Electronic address:

Accurate waste classification is key to successful waste management. However, most current studies have focused exclusively on single-label waste classification from images, which goes against common sense. In this paper, we move beyond single-label waste classification and propose a benchmark for evaluating the multi-label waste classification and localization tasks to advance waste management via deep learning-based methods. We propose a multi-task learning architecture (MTLA) based on a convolutional neural network, which can be used to simultaneously identify and locate wastes in images. The MTLA comprises a backbone network with proposed attention modules, a novel multi-level feature pyramid network, and a group of joint learning multi-task subnets. To achieve joint optimization of waste identification and location, we designed the loss functions according to the concepts of focusing and joint. The proposed MTLA achieved performance similar to that of experts and had high scores for multiple tasks related to waste management. Its F1 score exceeded 95.50% (95.12% to 95.88%, with a 95% confidence interval) on the multi-label waste classification task, and the average precision score was over 81.50% (@IoU = 0.5) on the waste localization task. To improve interpretation, heatmaps were used to visualize the salient features extracted by the MTLA. The proposed MTLA is a promising auxiliary tool that can improve the automation of waste management systems.
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http://dx.doi.org/10.1016/j.wasman.2021.03.017DOI Listing
May 2021

Melatonin ameliorates cypermethrin-induced impairments by regulating oxidative stress, DNA damage and apoptosis in porcine Sertoli cells.

Theriogenology 2021 Jun 19;167:67-76. Epub 2021 Mar 19.

Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China. Electronic address:

Cypermethrin (CYP) is a widely used insecticide that may be harmful to nontarget species. However, the toxicity of CYP to porcine Sertoli cells (SCs) and its associated mechanism is not known. We investigated the toxicity of CYP and showed that CYP induced cytotoxicity in porcine SCs in a dose-dependent manner. Mechanistic investigations revealed that CYP induced oxidative stress and DNA damage in porcine SCs, which provoked mitochondria-associated apoptosis. CYP also stimulated the phosphorylation of c-Jun N-terminal kinase (JNK) to induce porcine SC apoptosis and inhibited cell proliferation via the inhibition of nuclear factor kappa B (NFκB) expression. The natural antioxidant melatonin had an obvious protective effect against CYP-induced porcine SC toxicity. Overall, our results reveal that the mechanism underlying CYP-induced toxicity in porcine SCs involves oxidative stress, DNA damage, and apoptosis and suggest that melatonin may be used as a highly effective protective agent against oxidative stress.
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http://dx.doi.org/10.1016/j.theriogenology.2021.03.011DOI Listing
June 2021

A review on the role of plant in pharmaceuticals and personal care products (PPCPs) removal in constructed wetlands.

Sci Total Environ 2021 Aug 21;780:146637. Epub 2021 Mar 21.

Institute of Blue and Green Development, Weihai Institute of Interdisciplinary Research, Shandong University, Weihai 264209, China.

Pharmaceuticals and personal care products (PPCPs) cause ongoing water pollution and consequently have attracted wide attention. Constructed wetlands (CWs) show good PPCP removal performance through combined processes of substrates, plants, and microorganisms; however, most published research focuses on the role of substrates and microorganisms. This review summarizes the direct and indirect roles of wetland plants in PPCP removal, respectively. These direct effects include PPCP precipitation on root surface iron plaque, and direct absorption and degradation by plants. Indirect effects, which appear more significant than direct effects, include enhancement of PPCP removal through improved rhizosphere microbial activities (more than twice as much as bulk soil) stimulated by radial oxygen loss and exudate secretions, and the formation of supramolecular ensembles from PPCPs and humic acids from decaying plant materials which improving PPCPs removal efficiency by up to four times. To clarify the internal mechanisms of PPCP removal by plants in CWs, factors affecting wetland plant performance were reviewed. Based on this review, future research needs have been identified.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146637DOI Listing
August 2021

Antifouling Silicone Hydrogel Contact Lenses with a Bioinspired 2-Methacryloyloxyethyl Phosphorylcholine Polymer Surface.

ACS Omega 2021 Mar 26;6(10):7058-7067. Epub 2021 Feb 26.

Alcon Vision LLC, Fort Worth, Texas 76134, United States.

Inspired by the cell membrane surface as well as the ocular tissue, a novel and clinically applicable antifouling silicone hydrogel contact lens material was developed. The unique chemical and biological features on the surface on a silicone hydrogel base substrate were achieved by a cross-linked polymer layer composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), which was considered important for optimal on-eye performance. The effects of the polymer layer on adsorption of biomolecules, such as lipid and proteins, and adhesion of cells and bacteria were evaluated and compared with several conventional silicone hydrogel contact lens materials. The MPC polymer layer provided significant resistance to lipid deposition as visually demonstrated by the three-dimensional confocal images of whole contact lenses. Also, fibroblast cell adhesion was decreased to a 1% level compared with that on the conventional silicone hydrogel contact lenses. The movement of the cells on the surface of the MPC polymer-modified lens material was greater compared with other silicone hydrogel contact lenses indicating that lubrication of the contact lenses on ocular tissue might be improved. The superior hydrophilic nature of the MPC polymer layer provides improved surface properties compared to the underlying silicone hydrogel base substrate.
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http://dx.doi.org/10.1021/acsomega.0c06327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970573PMC
March 2021

Glycine alleviates fluoride-induced oxidative stress, apoptosis and senescence in a porcine testicular Sertoli cell line.

Reprod Domest Anim 2021 Jun 25;56(6):884-896. Epub 2021 Mar 25.

Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.

Glycine is a well-known free radical scavenger in the cellular antioxidant system that prevents oxidative damage and apoptosis. Excessive fluoride exposure is associated with multiple types of cellular damage in humans and animals. The objective of the present study was to investigate the protective effects of glycine on sodium fluoride (NaF) exposure and the possible underlying mechanisms in a porcine testicular Sertoli cell line model. Cellular viability and proliferation were examined following NaF exposure and glycine supplementation, and glycine dramatically ameliorated the decreases in NaF-induced porcine testicular Sertoli cell viability and proliferation. Further investigations revealed that glycine decreased NaF-induced intracellular reactive oxygen species production, DNA fragment accumulation and the apoptosis incidence in the porcine testicular Sertoli cell line; in addition, glycine improved mitochondrial function and ATP production. Notably, results of the SPiDER-β-Gal analysis suggested that glycine alleviated NaF-induced cellular senescence and downregulated P53, P21, HMGA2 and P16INK4a gene expression in the porcine testicular Sertoli cell line. Collectively, the beneficial effects of glycine alleviate NaF-induced oxidative stress, apoptosis and senescence, and together with our previous findings, support the hypothesis that glycine plays an important role in protecting against NaF exposure-induced impairments in the porcine testicular Sertoli cell line.
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http://dx.doi.org/10.1111/rda.13930DOI Listing
June 2021

Mn oxides changed nitrogen removal process in constructed wetlands with a microbial electrolysis cell.

Sci Total Environ 2021 May 23;770:144761. Epub 2021 Jan 23.

Institute of Blue and Green Development, Weihai Institute of Interdisciplinary Research, Shandong University, Weihai 264209, China.

Intensified Mn redox cycling could enhance nutrient removal in constructed wetlands (CWs). In this study, Mn oxides (birnessite-coated sand) were used as the matrix in horizontal flow CWs (HFCWs) with a microbial electrolysis cell (MEC) (E-B-CW) or without an MEC (B-CW). The model CWs were developed to investigate the capacities and mechanisms of nitrogen removal with increased Mn redox cycling. The results showed that E-B-CW had the highest average removal efficiencies for NH-N, NO-N and TN, followed by B-CW and control HFCW (C-CW). The Mn(III) oxides (MnOOH or MnO) and the Mn(IV) oxide (MnO) were all detected in E-B-CW and B-CW, while the matrix in E-B-CW had much more Mn(IV) oxides than B-CW. Interestingly, clustering heat map showed that ammonification and nitrate reduction were related to Mn-oxidizing bacteria and the relative abundance of Mn-oxidizing bacteria in E-B-CW was highest due to the re-oxidation of Mn(II) by the MEC.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144761DOI Listing
May 2021

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids facilitates the repair of cardiotoxin-induced skeletal muscle injury in mice.

Aging (Albany NY) 2021 03 10;13(6):8454-8466. Epub 2021 Mar 10.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, PR China.

In this study, we investigated the beneficial effects of high endogenous levels of n-3 polyunsaturated fatty acids (PUFAs) on skeletal muscle repair and regeneration using a mouse cardiotoxin (CTX, 20 μM/200 μL) -induced gastrocnemius muscle injury model. Transgenic mice expressing the gene, encoding n-3 fatty acid desaturase, showed higher n-3 PUFA levels and lower n-6/n-3 PUFA ratios in gastrocnemius muscle tissues. Hematoxylin and eosin and Masson's trichrome staining of gastrocnemius sections revealed increased muscle fiber size and reduced fibrosis in mice on days 7 and 14 after CTX injections. Gastrocnemius muscle tissues from mice showed reduced inflammatory responses and increased muscle fiber regeneration reflecting enhanced activation of satellite cells on day 3 after cardiotoxin injections. Gastrocnemius muscle tissues from cardiotoxin-treated mice showed reduced levels of pro-apoptotic proteins (Caspase 3 and Bax) and increased levels of anti-apoptotic proteins (Bcl-2 and Survivin). Moreover, eicosapentaenoic acid (EPA) reduced the incidence of apoptosis among cardiotoxin-treated C2C12 mouse myoblasts. These findings demonstrate that higher endogenous n-3 PUFA levels in mice enhances skeletal muscle repair and regeneration following cardiotoxin-induced injury.
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http://dx.doi.org/10.18632/aging.202655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034919PMC
March 2021