Publications by authors named "Shuang Li"

1,864 Publications

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Study on the genetic damage caused by cadmium sulfide quantum dots in human lymphocytes.

Open Life Sci 2022 11;17(1):463-472. Epub 2022 May 11.

School of Public Health, North China University of Science and Technology, No. 21 Bohai Road, Caofeidian, Tangshan, Hebei, China.

Cadmium sulfide quantum dots (CdS QDs) are being developed for sensors, fluorescent probes, and other platforms and are attracting increasing attention. Given the growing demand for QDs, it is clear that there is a need to understand their potential toxicity to organisms. However, little is known regarding the genotoxicity of CdS QDs to humans. Therefore, this study used CdS QDs as the research object, cultured human peripheral blood lymphocytes, and randomly divided them into a control group, CdS I group (CdS QDs), and CdS II group (CdS QDs coated with thioglycolic acid). After cultivation, we measured the olive tail distance, tail length, tail DNA%, lymphocyte micronucleus rate, and aneuploid rate. The comet test results indicated that the indices of the QD group were significantly larger than those of the control group ( < 0.05). The results of the micronucleus and chromosome aberration tests showed that the lymphocyte micronucleus rate and chromosome aneuploid rate in the QD group were significantly increased ( < 0.05) compared with those in the control group. In conclusion, CdS QDs have certain genotoxicity to human peripheral blood lymphocytes, and the DNA damage caused by CdS QDs encapsulated with thioglycolic acid is less severe than that caused by nonencapsulated CdS QDs.
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http://dx.doi.org/10.1515/biol-2022-0054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096230PMC
May 2022

Piezo1 activation facilitates ovarian cancer metastasis via Hippo/YAP signaling axis.

Channels (Austin) 2022 12;16(1):159-166

Department of Pathology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei China.

Ovarian cancer (OC) is a highly malignant cancer with great metastatic potential. Here we aimed to investigate the role of Piezo1, a gene related to the mechanical environment of the tumor, in promoting the metastasis of OC. We performed Piezo1 knockdown in A-1847 cells using small hairpin RNAs, and the cells were inoculated subcutaneously in nude mice. Piezo1 knockdown decreased the tumor growth rate of OC tumor xenografts in mice and reduced cell migration . Metastasis in the lung was also attenuated after Piezo1 knockdown as revealed by HE staining of the lung tissues, which was concomitant with downregulation of E-Cadherin and vimentin and upregulation of N-Cadherin analyzed using western blot analysis, suggesting suppressed epithelial-to-mesenchymal transition. Migration of Piezo1-knockdown cells was also analyzed for their migratory capabilities using the scratch assay. We also analyzed the key proteins in the Hippo/YAP signaling pathway using western blot after treating A-1847 and 3AO cells with a Piezo1 inducer, Yoda1. Piezo1 inducer Yoda1 activated Hippo/YAP signal in OC cells. In conclusion, Piezo1 is overexpressed in OC tissues and contributes to OC tumor growth and metastasis. Suppression of Piezo1 is a potential therapeutic strategy for OC.
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http://dx.doi.org/10.1080/19336950.2022.2099381DOI Listing
December 2022

Relationship between Traditional Chinese Medicine Syndrome Elements and Prognosis of Patients with IgA Nephropathy.

Evid Based Complement Alternat Med 2022 30;2022:2270406. Epub 2022 Jul 30.

National Clinical Research Center for Kidney Diseases, State Key Laboratory of Kidney Diseases, Beijing Key Laboratory of Kidney Disease Research, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People's Liberation Army, Beijing 100853, China.

Objective: It is not clear which Traditional Chinese Medicine- (TCM-) related elements affect primary IgA nephropathy (IgAN) progression. Here, we explored the risk factors, based on TCM syndrome elements, related to the prognosis of primary IgAN patients.

Methods: We analyzed patients with newly diagnosed, biopsy-proven IgAN at a single institution from December 2013 to September 2021. Basic clinical and pathological characteristics were assessed at the time of renal biopsy. The study endpoint was end-stage renal disease (ESRD: eGFR <15 ml/min per 1.73 m, dialysis, or kidney transplantation) and/or eGFR decreased by >30% from baseline. Kaplan‒Meier survival analysis was used to explore the role of TCM syndrome elements in IgAN progression. Multivariate Cox regression analysis with adjustment for traditional risk factors was performed to explore TCM syndrome elements that may influence patient prognosis. The factors correlated with TCM syndrome elements in IgAN patients were further evaluated by logistic regression analysis.

Results: During a median follow-up of 22.0 months, 53 (12.5%) of the 423 included IgAN patients reached the study endpoint. The main IgAN disease location elements were the kidney, liver, and spleen. The main IgAN disease nature elements were Yin-deficiency and Qi-deficiency, dampness, Yang-deficiency, phlegm, and Blood-deficiency. Kaplan‒Meier analysis identified three disease locations (liver, spleen, and kidney) and four disease natures (Qi-deficiency, Yang-deficiency, phlegm, and dampness) as elements associated with poor renal survival in IgAN patients. In multivariate Cox regression analysis, baseline Yang-deficiency was an independent risk predictor of poor prognosis in primary IgAN patients (hazard ratio 2.338; 95% confidence interval [CI]: 1.208-4.525; =0.012) after adjustment for traditional risk factors. Furthermore, logistic regression analysis identified being female (odds ratio [OR] 2.518; 95% CI: 1.538-4.122; < 0.001), older age (OR 1.043; 95% CI: 1.022-1.065; < 0.001), low hemoglobin levels (OR 0.984; 95% CI: 0.971-0.996; =0.013), and cellular/fibrocellular crescents (OR 1.706; 95% CI: 1.068-2.728; =0.026) as factors affecting Yang-deficiency in IgAN patients.

Conclusions: Yang-deficiency independently predicts the risk of poor prognosis in primary IgAN patients. Being female, older age, low hemoglobin levels, and cellular/fibrocellular crescents were independently associated with Yang-deficiency in IgAN patients.
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http://dx.doi.org/10.1155/2022/2270406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356779PMC
July 2022

Dual-strategy biosensing of glucose based on multifunctional CuWO nanoparticles.

Analyst 2022 Aug 8. Epub 2022 Aug 8.

College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.

In this research, dual-strategy biosensing of glucose was proposed based on multifunctional CuWO nanoparticles (CuWO NPs), which were prepared for the application of electrochemical and colorimetric sensing of glucose. CuWO NPs show large specific surface area and good conductivity as well as excellent peroxidase-like activity. A sensitive and selective electrochemical glucose biosensor was fabricated with the immobilization of glucose oxidase (GOx) on a CuWO NP modified electrode for enhancing the direct electron transfer behavior. A wide linear range of 0.005-1.8 mM with a low detection limit of 1.5 μM and a high sensitivity of 28.02 mA M cm were achieved by using the electrochemical biosensor. Meanwhile, a colorimetric and visual glucose biosensor was constructed based on the GOx/CuWO cascade nanozyme, which shows a linear range of 0.05-1.0 mM with excellent selectivity. CuWO NPs as a promising matrix open up a dual-strategy biosensor for sensitive and selective detection of glucose.
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http://dx.doi.org/10.1039/d2an01003hDOI Listing
August 2022

"Three-in-one" nanohybrids as synergistic nanozymes assisted with exonuclease I amplification to enhance colorimetric aptasensor for ultrasensitive detection of kanamycin.

Anal Chim Acta 2022 Aug 19;1222:340178. Epub 2022 Jul 19.

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, China. Electronic address:

Kanamycin (KAN) residues in animal-derived food can cause serious threats to human health. Herein, a colorimetric aptasensor was described using "three-in-one" nanohybrids ([email protected]/PtNP) as synergistic nanozymes assisted with the exonuclease I (Exo I) signal amplification for the ultrasensitive detection of KAN. In the presence of KAN and Exo I, the KAN aptamer (APT) was specifically bound to KAN, and the remaining APT complementary strand (cDNA) captured [email protected]/PtNP labeled with the cDNA complementary strand (cDNA). Due to the synergistic effect of hemin, Fe-MIL-88NH and platinum nanoparticles (PtNPs), [email protected]/PtNP exhibited superior catalytic performance and could efficiently catalyze the 3,3',5,5'-tetramethylbenzidine (TMB)-HO system for signal development. Moreover, Exo I could digest APT and release KAN into a new cycle for signal amplification. Based on multiple signal amplification effects, our proposed aptasensor achieved a wide detection range from 0.01 to 100 ng mL with a low detection limit of 2 pg mL. This assay also successfully detected KAN-added milk and shrimp samples with added recovery ranges of 93.58%-106.08% and relative standard deviations (RSDs) of 2.20%-5.50%. The aptasensor enabled ultrasensitive, specific, and fast detection of KAN, and exhibited promising applications in the efficient detection of food pollutants.
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http://dx.doi.org/10.1016/j.aca.2022.340178DOI Listing
August 2022

Sample-multiplexing approaches for single-cell sequencing.

Cell Mol Life Sci 2022 Aug 5;79(8):466. Epub 2022 Aug 5.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, China.

Single-cell sequencing is widely used in biological and medical studies. However, its application with multiple samples is hindered by inefficient sample processing, high experimental costs, ambiguous identification of true single cells, and technical batch effects. Here, we introduce sample-multiplexing approaches for single-cell sequencing in transcriptomics, epigenomics, genomics, and multiomics. In single-cell transcriptomics, sample multiplexing uses variants of native or artificial features as sample markers, enabling sample pooling and decoding. Such features include: (1) natural genetic variation, (2) nucleotide-barcode anchoring on cellular or nuclear membranes, (3) nucleotide-barcode internalization to the cytoplasm or nucleus, (4) vector-based barcode expression in cells, and (5) nucleotide-barcode incorporation during library construction. Other single-cell omics methods are based on similar concepts, particularly single-cell combinatorial indexing. These methods overcome current challenges, while enabling super-loading of single cells. Finally, selection guidelines are presented that can accelerate technological application.
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http://dx.doi.org/10.1007/s00018-022-04482-0DOI Listing
August 2022

Enhanced Ion Rejection in Carbon Nanotubes by a Lateral Electric Field.

Langmuir 2022 Aug 3. Epub 2022 Aug 3.

Reverse osmosis membranes hold great promise for dealing with global water scarcity. However, the trade-off between ion selectivity and water permeability is a serious obstacle to desalination. Herein, we introduce an effective strategy to enhance the desalination performance of the membrane. A series of molecular dynamics simulations manifest that an additional lateral electric field significantly promotes ion rejection in carbon nanotubes (CNTs) under the drive of longitudinal pressure. Specifically, with the increase in the electric field, the ion flux shows a deep linear decay, while the water flux decreases only slightly, resulting in a linear increase in ion rejection. The energy barriers of ions around the CNT inlet are obtained by calculating the potentials of mean force to explain enhanced ion rejection. The lateral electric field uniformly raises the energy barriers of ions by pushing them away from the CNT inlet, corresponding to the enhanced ion velocity in the field direction. Furthermore, with the increase in CNT diameter, there is a significant increase in the flux of both ions and water; however, the lateral electric field can also obviously enhance the ion rejection in wider CNTs. Consequently, the enhancement of ion rejection by lateral electric fields should be universal for different CNT diameters, which opens a new avenue for selective permeation and may have broad implications for desalination devices with large pore sizes.
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http://dx.doi.org/10.1021/acs.langmuir.2c01780DOI Listing
August 2022

Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of auricularia auricular-judae polysaccharide.

Int J Occup Med Environ Health 2022 Jul 26. Epub 2022 Jul 26.

North China University of Science and Technology, Tangshan, People's Republic of China (School of Psychology and Mental Health).

Objectives: The aim of the paper is to explore the role of lung microbiome disorder in lung tissue injury induced by exposure to particulate matter with a maximum diameter of 2.5 μm (PM2.5) and the alleviation effect of auricularia auricular-judae polysaccharide (AAP).

Material And Methods: Sprague Dawley rats were given PM suspension at a dose of 20 mg/l twice a week for 8 weeks. Then, 100 mg/kg or 200 mg/kg of AAP was administered to the rats after PM exposure. The bronchoalveolar lavage fluid (BALF) and lung tissue samples were collected at the end of the experiment. The BALF was meant to detect changes in lung microbiome by 16S sequences and cluster analysis, with the application of the principal component analysis and the partial least squares discriminant analysis. The levels of interferon-γ (IFN-γ), and interleukin (IL)-4, IL-8, and IL-10 in lung tissue were detected by the enzyme-linked immunosorbent assay method. The pathological changes in lung tissue were observed by hematoxylin and eosin staining.

Results: After PM exposure, the alveolar septum was widened, and the structures of alveolar walls were destroyed. There was inflammatory cells infiltration in the alveolar space and the interstitial space. Alpha diversity in BALF showed that the Chao1, ACE, Simpson, and Shannon values were increased, and the lung microbiome analysis revealed that the relative abundance of and increased, while the relative abundance of and decreased. The contents of IFN-γ and IL-8 in lung tissue increased while the content of IL-10 decreased. After the administration of AAP, the alveolar structure damage was alleviated, and the interstitial hemorrhage, edema, and inflammatory cells infiltration were reduced. The Chao1 and ACE values decreased, and the taxonomic abundance values of were much higher. Simultaneously, the contents of IFN-γ, IL-4, and IL-8 decreased, and the content of IL-10 increased.

Conclusions: It was found that PM resulted in lung microbiome disorder, which might lead to the inflammation of lung tissue. It was also revealed that AAP could alleviate the inflammatory damage of lung tissue induced by PM.
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http://dx.doi.org/10.13075/ijomeh.1896.01742DOI Listing
July 2022

Noninvasively Assessed Portal Hypertension Grade Predicts Post-Hepatectomy Liver Failure in Patients With HepatocellCarcinoma: A Multicenter Study.

Front Oncol 2022 14;12:934870. Epub 2022 Jul 14.

Center of Portal Hypertension, Department of Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

Purpose: To determine the predictive value of portal hypertension (PH) for the development of post-hepatectomy liver failure (PHLF) in patients with hepatocellular carcinoma (HCC).

Patients And Methods: This study enrolled a total of 659 patients with HCC that received hepatectomy as a first-line therapy. PH was classified as grade 0, 1, and 2 according to whether the indirect criteria for PH were met: 1) patients had obvious varicose veins and 2) splenomegaly was present and platelet count < 100 × 10/L. The effects of each variable on the occurrence of PHLF were assessed using univariate and multivariate analyses.

Results: PH grade 2 (odds ratio [OR] = 2.222, p = 0.011), higher age (OR = 1.031, p = 0.003), hepatitis C infection (OR = 3.711, p = 0.012), open surgery (OR = 2.336, p < 0.001), portal flow blockage (OR = 1.626, p = 0.023), major hepatectomy (OR = 2.919, p = 0.001), hyperbilirubinemia (≥ 17.2 μmol/L, OR = 2.113, p = 0.002), and high levels of alpha-fetoprotein (> 400n g/ml, OR = 1.799, p = 0.008) were significantly associated with PHLF occurrence. We performed a subgroup analysis of liver resection and found that the extent of liver resection and PH grade were good at distinguishing patients at high risk for PHLF, and we developed an easy-to-view roadmap.

Conclusion: PH is significantly related to the occurrence of PHLF in patients who underwent hepatectomy. Noninvasively assessing PH grade can predict PHLF risk.
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http://dx.doi.org/10.3389/fonc.2022.934870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333899PMC
July 2022

Main Factors Influencing the Gut Microbiota of Datong Yaks in Mixed Group.

Animals (Basel) 2022 Jul 11;12(14). Epub 2022 Jul 11.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Coastal Ecosystems Research Station of the Yangtze River Estuary, School of Life Sciences, Fudan University, Shanghai 200433, China.

The Datong yak () is the first artificial breed of yaks in the world and has played an important role in the improvement of domestic yak quality on the Qinghai-Tibet Plateau. The Datong yak breeding farm in the Qinghai province of China is the main place for the breeding and feeding of Datong yaks. It hosts domestic Datong yaks and wild male yaks, mainly in mixed groups. Different managements have different effects on livestock. The gut microbiota is closely related to the health and immunity of Datong yaks, and mixed grouping can affect the composition and diversity of the gut microbiota of Datong yaks. To reveal the effects of mixed grouping on the gut microbiota of Datong yaks and wild yaks and identify the main dominant factors, we compared the gut microbial diversities of domestic males and females and wild males based on 16S rRNA V3-V4 regions using fresh fecal samples. The data showed significant differences in the gut microbial diversity of these three groups, and the α-diversity was the highest in wild males. Different factors influence the gut microbiota, and the main influencing factors were different in different groups, including sex differences, host genetics, and physical interactions. We also compared ecological assembly processes in the three groups. The results showed that mixed grouping contributed to the improvement of gut microbial diversity in domestic females. Our study provides effective and feasible suggestions for the feeding and management of the Datong yaks.
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http://dx.doi.org/10.3390/ani12141777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312300PMC
July 2022

Carbodefluorination of fluoroalkyl ketones via a carbene-initiated rearrangement strategy.

Nat Commun 2022 Jul 25;13(1):4280. Epub 2022 Jul 25.

Department of Chemistry, Northeast Normal University, Changchun, 130024, China.

The C-F bond cleavage and C-C bond formation (i.e., carbodefluorination) of readily accessible (per)fluoroalkyl groups constitutes an atom-economical and efficient route to partially fluorinated compounds. However, the selective mono-carbodefluorination of trifluoromethyl (CF) groups remains a challenge, due to the notorious inertness of C-F bond and the risk of over-defluorination arising from C-F bond strength decrease as the defluorination proceeds. Herein, we report a carbene-initiated rearrangement strategy for the carbodefluorination of fluoroalkyl ketones with β,γ-unsaturated alcohols to provide skeletally and functionally diverse α-mono- and α,α-difluoro-γ,δ-unsaturated ketones. The reaction starts with the formation of silver carbenes from fluoroalkyl N-triftosylhydrazones, followed by nucleophilic attack of a β,γ-unsaturated alcohol to form key silver-coordinated oxonium ylide intermediates, which triggers selective C-F bond cleavage by HF elimination and C-C bond formation through Claisen rearrangement of in situ generated difluorovinyl ether. The origin of chemoselectivity and the reaction mechanism are determined by experimental and DFT calculations. Collectively, this strategy by an intramolecular cascade process offers significant advances over existing stepwise strategies in terms of selectivity, efficiency, functional group tolerance, etc.
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http://dx.doi.org/10.1038/s41467-022-31976-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314321PMC
July 2022

Tailoring Bond Microenvironments and Reaction Pathways of Single-Atom Catalysts for Efficient Water Electrolysis.

Angew Chem Int Ed Engl 2022 Jul 25. Epub 2022 Jul 25.

Sichuan University, Department of polymer science, No. 24, Yihuan Road, 610065, Chengdu, CHINA.

Single-Atom Sites (SASs) are commonly stabilized and influenced by neighboring atoms in the host; disclosing the structure-reactivity relationships of SASs in water electrolysis are the grand challenges originating from the enormous support materials with complex structures. Through a multidisciplinary view of the design principles, synthesis strategies, characterization techniques, and theoretical analysis of structure-performance correlations, this timely review is dedicated to summarizing the most recent progress in tailoring bond microenvironments on different supports and discussing the reaction pathways and performance advantages of different SAS structures for water electrolysis . The essences and mechanisms of how SAS structures influence their electrocatalysis and the critical needs for their future developments are discussed. Finally, the challenges and perspectives are also provided to stimulate their practically widespread utilization in water-splitting electrolyzers.
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http://dx.doi.org/10.1002/anie.202208667DOI Listing
July 2022

The Effects of Resistin Gene Polymorphism on Pain Thresholds and Postoperative Sufentanil Consumption in Gastric Cancer Patients.

J Pain Res 2022 17;15:1995-2004. Epub 2022 Jul 17.

Department of Anesthesiology, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China.

Purpose: As an adipocyte-secreted hormone, resistin is linked to inflammation, insulin resistance and atherosclerosis. Currently, resistin is proposed as a novel biomarker for postoperative pain intensity. However, due to the various types of surgery and limited numbers of studies, previous conclusions should be validated. This study aimed to explore the effect of resistin polymorphism (rs3745367) on pain thresholds and sufentanil consumption in gastric cancer patients.

Patients And Methods: A total of 148 gastric cancer patients enrolled in this study had their pain thresholds measured before surgery. After the exclusion of 16 patients, the characteristics of demography and clinic, numerical rating scale (NRS) and sufentanil consumption of 132 patients were recorded. Rs3745367 of resistin was identified by Sanger sequencing. Multivariate linear regression analysis was performed for sufentanil consumption and mechanical pain threshold.

Results: The distributions of the GG, AG, and AA genotypes of rs3745367 among the participants were 54 (40.9%), 65 (49.2%), and 13 (9.9%), respectively. The mechanical pain threshold (P=0.04) and postoperative sufentanil consumption in the 1st 24 h (P=0.03) were significantly different among GG, AG, and AA genotype carriers. There was no significant difference among the three genotypes for the heat pain threshold and cold pain threshold. Regarding the NRS, no statistically significant difference among the three different genotypes was found 24 h postoperatively.

Conclusion: Rs3745367 of resistin is associated with the mechanical pain threshold and postoperative sufentanil consumption in gastric cancer patients. Patients with the AA genotype of rs3745367 present an increased mechanical pain threshold and decreased postoperative sufentanil consumption.
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http://dx.doi.org/10.2147/JPR.S372845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304898PMC
July 2022

Self-Expandable Metal Stent in the Management of Malignant Airway Disorders.

Front Med (Lausanne) 2022 7;9:902488. Epub 2022 Jul 7.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Self-expanding metallic stent (SEMS) is a palliative therapy for patients with malignant central airway obstruction (CAO) or tracheoesophageal fistula (TEF). Despite this, many patients experience death shortly after SEMS placement.

Aims: We aimed to investigate the effect of SEMS on the palliative treatment between malignant CAO and malignant TEF patients and investigate the associated prognostic factors of the 3-month survival.

Methods: We performed a single-center, retrospective study of malignant CAO or TEF patients receiving SEMS placement. Clinical data were collected using the standardized data abstraction forms. Data were analyzed using SPSS 22.0. A two-sided -value <0.05 was statistically significant.

Results: 106 malignant patients (82 CAO and 24 TEF) receiving SEMS placement were included. The body mass index (BMI), hemoglobin levels, and albumin levels in the malignant TEF group were lower than in the malignant CAO group (all < 0.05). The procalcitonin levels, C-reactive protein levels, and the proportion of inflammatory lesions were higher in the malignant TEF group than in the malignant CAO group (all < 0.05). The proportion of symptomatic improvement after the SEMS placement was 97.6% in the malignant CAO group, whereas 50.0% in the malignant TEF group, with a significant difference ( = 0.000). Three months after SEMS placement, the survival rate at was 67.0%, significantly lower in the malignant TEF group than in the malignant CAO group (45.8% vs. 73.2%, = 0.013). Multivariate analysis revealed that BMI [odds ratio (OR) = 1.841, 95% certificated interval (CI) (1.155-2.935), = 0.010] and neutrophil percentage [OR = 0.936, 95% CI (0.883-0.993), = 0.027] were the independent risk factors for patients who survived three months after SEMS placement.

Conclusions: We observed symptom improvement in malignant CAO and TEF patients after SEMS placement. The survival rate in malignant TEF patients after SEMS placement was low, probably due to aspiration pneumonitis and malnutrition. Therefore, we recommend more aggressive treatment modalities in patients with malignant TEF, such as strong antibiotics, nutrition support, and strategic ventilation. More studies are needed to investigate the prognostic factors in patients with malignant airway disorders receiving SEMS placement.
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http://dx.doi.org/10.3389/fmed.2022.902488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302573PMC
July 2022

-2-(Phenylamino) Benzamide Derivatives as Dual Inhibitors of COX-2 and Topo I Deter Gastrointestinal Cancers via Targeting Inflammation and Tumor Progression.

J Med Chem 2022 Aug 22;65(15):10481-10505. Epub 2022 Jul 22.

School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang421001, Hunan, China.

Given the close association between inflammation and cancer, combining anti-inflammation therapy is prominent to improve the anticancer effect. Based on , a series of agents targeting COX-2 and Topo I were designed by combining fenamates and phenols. The optimal compound displayed an enhanced inhibitory effect on COX-2 compared to tolfenamic acid and and showed better inhibition of Topo I than . Importantly, showed potential anticancer effects and suppressed the activation of the NF-κB pathway in cancer cells. inhibited the nuclear translocation of NF-κB and suppressed the production of NO, COX-2, and IL-1β in RAW264.7. In vivo, showed acceptable pharmacokinetic parameters, decreased the tumor growth without affecting the body weight, down-regulated COX-2 and MMP-9, and induced apoptosis in the CT26.WT tumor-bearing mice. Accordingly, as a potential Topo I/COX-2 inhibitor which possessed anti-inflammatory and anticancer effects, with inhibition of the NF-κB pathway, is promising for gastrointestinal cancer therapy.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00635DOI Listing
August 2022

Kinetic Diagnostics and Synthetic Design of Platinum Group Metal-Free Electrocatalysts for the Oxygen Reduction Reaction Using Reactivity Maps and Site Utilization Descriptors.

J Am Chem Soc 2022 Aug 21;144(30):13487-13498. Epub 2022 Jul 21.

The Electrochemical Catalysis, Energy and Materials Science Laboratory, Department of Chemistry, Technical University Berlin, Straße des 17. Juni 142, 10623 Berlin, Germany.

The experimental development of catalytically ever-more active platinum group metal (PGM)-free materials for the oxygen reduction reaction (ORR) at fuel cell cathodes has been until recently a rather empirical iteration of synthesis and testing. Here, we present how kinetic reactivity maps based on kinetic descriptors of PGM-free single-metal-site ORR electrocatalysts can help to better understand the origin of catalytic reactivity and help to derive rational synthetic guidelines toward improved catalysts. Key in our analysis are the catalytic surface site density (SD) and the catalytic turnover frequency (TOF) in their role as controlling kinetic parameters for the ORR reactivity of PGM-free nitrogen-coordinated single-metal M-site carbon (MNC) catalysts. SD-TOF plots establish two-dimensional reactivity maps. We also consider the ratio between SD and the total number of single-metal sites in the bulk, referred to as the site utilization factor, which we propose as another guiding parameter for optimizing the synthesis of MNC catalysts. Exemplified by two sets of FeNC, CoNC, and SnNC catalysts prepared using two distinctly different N- and C-precursor material classes (Zn-based zeolitic imidazolate frameworks and covalent polyaniline), we comparatively diagnose the intrinsic kinetic ORR parameters as well as structural, morphological, and chemical properties. From there, we derive and discuss possible synthetic guidelines for further improvements. Our approach can be extended to other families of catalysts and may involve kinetic performance data of idealized liquid-electrolyte cells as well as gas diffusion layer-type flow cells.
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http://dx.doi.org/10.1021/jacs.2c01594DOI Listing
August 2022

T1 signal intensity ratio of the pancreas as an imaging biomarker for the staging of chronic pancreatitis.

Abdom Radiol (NY) 2022 Jul 20. Epub 2022 Jul 20.

Department of Medicine, Division of Gastroenterology, Hepatology & Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Purpose: Our purpose was to validate the T1 SIR (T1 score) as an imaging biomarker for the staging of CP in a large, multi-institutional, prospective study.

Methods: The prospective study population included 820 participants enrolled in the PROCEED study from nine clinical centers between June 2017 and December 2021. A radiologist at each institution used a standardized method to measure the T1 signal intensity of the pancreas and the reference organs (spleen, paraspinal muscle, liver), which was used to derive respective T1 scores. Participants were stratified according to the seven mechanistic stages of chronic pancreatitis (MSCP 0-6) based on their clinical history, MRCP, and CT findings.

Results: The mean pancreas-to-spleen T1 score was 1.30 in participants with chronic abdominal pain, 1.22 in those with acute or recurrent acute pancreatitis, and 1.03 in definite CP. After adjusting for covariates, we observed a linear, progressive decline in the pancreas-to-spleen T1 score with increasing MSCP from 0 to 6. The mean pancreas-to-spleen T1 scores were 1.34 (MSCP 0), 1.27 (MSCP 1), 1.21 (MSCP 2), 1.16 (MSCP 3), 1.18 (MSCP 4), 1.12 (MSCP 5), and 1.05 (MSCP 6) (p < 0.0001). The pancreas-to-liver and pancreas-to-muscle T1 scores showed less linear trends and wider confidence intervals.

Conclusion: The T1 score calculated by SIR of the pancreas-to-spleen shows a negative linear correlation with the progression of chronic pancreatitis. It holds promise as a practical imaging biomarker in evaluating disease severity in clinical research and practice.
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http://dx.doi.org/10.1007/s00261-022-03611-4DOI Listing
July 2022

Deep Learning-Based Mental Health Model on Primary and Secondary School Students' Quality Cultivation.

Authors:
Shuang Li Yu Liu

Comput Intell Neurosci 2022 6;2022:7842304. Epub 2022 Jul 6.

Department of Psychology, Guizhou Minzu University, Guiyang 550025, Guizhou, China.

The purpose was to timely identify the mental disorders (MDs) of students receiving primary and secondary education (PSE) (PSE students) and improve their mental quality. Firstly, this work analyzes the research status of the mental health model (MHM) and the main contents of PSE student-oriented mental health quality cultivation under deep learning (DL). Secondly, an MHM is implemented based on big data technology (BDT) and the convolutional neural network (CNN). Simultaneously, the long short-term memory (LSTM) is introduced to optimize the proposed MHM. Finally, the performance of the MHM before and after optimization is evaluated, and the PSE student-oriented mental health quality training strategy based on the proposed MHM is offered. The results show that the accuracy curve is higher than the recall curve in all classification algorithms. The maximum recall rate is 0.58, and the minimum accuracy rate is 0.62. The decision tree (DT) algorithm has the best comprehensive performance among the five different classification algorithms, with accuracy of 0.68, recall rate of 0.58, and 1-measure of 0.69. Thus, the DT algorithm is selected as the classifier. The proposed MHM can identify 56% of students with MDs before optimization. After optimization, the accuracy is improved by 0.03. The recall rate is improved by 0.19, the 1-measure is improved by 0.05, and 75% of students with MDs can be identified. Diverse behavior data can improve the recognition effect of students' MDs. Meanwhile, from the 60th iteration, the mode accuracy and loss tend to be stable. By comparison, batch_size has little influence on the experimental results. The number of convolution kernels of the first convolution layer has little influence. The proposed MHM based on DL and CNN will indirectly improve the mental health quality of PSE students. The research provides a reference for cultivating the mental health quality of PSE students.
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http://dx.doi.org/10.1155/2022/7842304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279049PMC
July 2022

Oseltamivir modified bovine serum albumin inhibits neuraminidase activity and accumulates virion particles to disturb influenza virus replication.

Carbohydr Res 2022 Jul 8;520:108631. Epub 2022 Jul 8.

Research Centre of Modern Analytical Technology, Tianjin University of Science & Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China; China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China. Electronic address:

The preparation of oseltamivir-bovine serum albumin conjugate (OS-BSA) for use as a multivalent influenza neuraminidase (NA) inhibitor is reported. Briefly, the oseltamivir azidohexyl ester was synthesized and covalently bound via an orthogonal attachment to bicyclononyne-modified BSA using copper-free click chemistry. Primary antiviral assays on NA protein and cellular levels showed that the synthetic multivalent OS-BSA conjugate was a more effective inhibitor than monomeric OS azidohexyl ester. Further investigation of the antiviral mechanism found that the prepared OS-BSA could not only be used as a multivalent NA inhibitor but also acted as an adsorbent for the aggregation of virion particles, contributing to the inhibition of the influenza viral replication cycle. Our findings provide insight into the antiviral mechanism of multivalent NA inhibitors and form a basis for the development of novel antiviral agents.
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http://dx.doi.org/10.1016/j.carres.2022.108631DOI Listing
July 2022

Incorporating soil mercury species and fractions into multi-objective risk assessment of a residue disposal site in China.

Environ Geochem Health 2022 Jul 15. Epub 2022 Jul 15.

National Engineering Research Centre of Urban Environmental Pollution Control, Beijing Key Laboratory for Risk Modeling and Remediation of Contaminated Sites, Beijing Municipal Research Institute of Eco-Environmental Protection, Beijing, 100037, China.

Environmental problems in soil and water caused by solid waste dumps have become a growing concern. This study proposes an integrated risk assessment model aimed at multi-objectives including human, ecology and groundwater and develops remediation target values at different tiers associated with soil mercury species and fractions in a typical residue disposal site of China. The results show that the residue disposal site was severely contaminated with mercury, with the maximum mercury content in the soil reaching 579.14 mg/kg. The average concentration of vapour mercury, bioaccessible mercury, bioavailable mercury and leachable mercury tested in laboratory was 87.65 mg/kg, 3.15 mg/kg (intestinal phase), 1.654 mg/kg and 0.045 mg/L, respectively. The hazard index calculated using total mercury, bioaccessible mercury and vapour mercury was 7.43 E + 01, 4.42 E + 01, and the remediation target values were7.79 mg/kg and 13.1 mg/kg, respectively. The ecological risk for total mercury and bioavailable mercury was calculated using measured site soil mercury background values of 6390.92 and 94.52, and the remediation target was 0.7 mg/kg and 47.33 mg/kg, respectively. Under Class IV water conditions, the measured and three-phase equilibrium model simulations of leachable mercury resulted in remediation targets of 6 mg/kg and 10 mg/kg for soil mercury. Compared to total mercury, the remediation target values calculated using mercury species and fractions were significantly larger under human health protection, ecology protection and groundwater protection. This results in a reduction in the area of soil to be remediated by 20.3-85.7%, resulting in significant savings in remediation costs. It was concluded that when conducting risk assessment and reuse of mercury-contaminated sites, it is important to consider the species and fractions of mercury in the soil in order to reasonably determine the remediation criteria and scale of remediation to avoid over-remediation and incomplete remediation. At the same time, a comprehensive protection target remediation mechanism should be established by combining different receptors.
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http://dx.doi.org/10.1007/s10653-022-01311-yDOI Listing
July 2022

Current through a hybrid four-terminal Josephson junction with Majorana nanowires.

J Phys Condens Matter 2022 Jul 26;34(39). Epub 2022 Jul 26.

Hebei College of Industry and Technology, Shijiazhuang, Hebei, 050091, People's Republic of China.

We investigate the current through a hybrid four-terminal Josephson junction with semiconductor nanowires, in which the junction is connected with two superconducting electrodes and two normal electrodes. The semiconductor nanowire, which is subject to an external magnetic field with Rashba spin-orbit coupling and proximity-induced superconductivity, can host Majorana bound states. When all the nanowires lie in topological nontrivial region, a 4-periodic current can be observed through the normal terminal and a 2-periodic current through the superconducting terminal. When a rotating magnetic field is applied to the junction, the supercurrent through different terminals varies with the variation of the magnetic field direction. Only when the magnetic field is applied at certain angles, we find that the 4-periodic current will appear through the normal terminal.
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http://dx.doi.org/10.1088/1361-648X/ac8131DOI Listing
July 2022

Biomechanical Evaluation of Spinal Column after Percutaneous Cement Discoplasty: A Finite Element Analysis.

Orthop Surg 2022 Aug 11;14(8):1853-1863. Epub 2022 Jul 11.

Department of Bone and Tissue Oncology, Tianjin Hospital, Tianjin University, Tianjin, China.

Objective: To compare the biomechanical properties of percutaneous cement discoplasty (PCD) in the spinal column between different implant-endplate friction.

Methods: A validated L3-Scarumfinite element (FE) model was modified for simulation. In the PCD model, the L4/5 level was modified based on model 1 (M1) and model 2 (M2). In M1, the interaction between bone cement and endplate was defined as face-to-face contact with a friction coefficient of 0.3; in M2, the contact was defined as a Tie constraint. 7.5 N m moments of four physiological motions and axial load of 15, 100 and 400 N preload were imposed at the top of L3. The range of motion (ROM) and interface stress analysis of endplates, annulus fibrosus and bone cement of the operated level were calculated for comparisons among the three models.

Results: The ROM of M1 and M2 increased when compared with the intact model during flexion (FL) (17.5% vs 10.0%), extension (EX) (8.8% vs -8.8%), left bending (LB) (19.0% vs -17.2%) and left axial rotation (LR) (34.6% vs -3.8%). The stress of annulus fibrosus in M1 and M2 decreased in FL (-48.4% vs -57.5%), EX (-25.7% vs -14.7%), LB (-47.5% vs -52.4%), LR (-61.4% vs -68.7%) and axis loading of 100 N (-41.5% vs -15.3%), and 400 N (-27.9% vs -27.3%). The stress of upper endplate of M1 and M2 increased in FL (24.6% vs 24.7%), LB (82.2% vs 89.5%), LR (119% vs 62.4%) and axis loading of 100 N (64.6% vs 45.5%), and 400 N (58.2% vs 24.3%), but was similar in EX (2.9% vs 0.3%). The stress of lower endplate of M1 and M2 increased in FL (170.9% vs 175.0%), EX (180.8% vs 207.7%), LB (302.6% vs 274.7%), LR (332.4% vs 132.8%) and axis loading of 100 N (350.7% vs 168.6%), and 400 N (165.2% vs 106.7%).

Conclusion: Percutaneous cement discoplasty procedure could make effect on the mobility or stiffness. The fusion of bone cement and endplate might have more biomechanical advantages, including of the decreasing rate of implant subsidence and dislocation, and the increase spine stability.
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http://dx.doi.org/10.1111/os.13314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363717PMC
August 2022

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases.

Signal Transduct Target Ther 2022 07 11;7(1):231. Epub 2022 Jul 11.

Department of Geriatrics, The Second Xiangya Hospital of Central South University, 410011, Changsha, Hunan, China.

Aging-induced alternations of vasculature structures, phenotypes, and functions are key in the occurrence and development of vascular aging-related diseases. Multiple molecular and cellular events, such as oxidative stress, mitochondrial dysfunction, vascular inflammation, cellular senescence, and epigenetic alterations are highly associated with vascular aging physiopathology. Advances in nanoparticles and nanotechnology, which can realize sensitive diagnostic modalities, efficient medical treatment, and better prognosis as well as less adverse effects on non-target tissues, provide an amazing window in the field of vascular aging and related diseases. Throughout this review, we presented current knowledge on classification of nanoparticles and the relationship between vascular aging and related diseases. Importantly, we comprehensively summarized the potential of nanoparticles-based diagnostic and therapeutic techniques in vascular aging and related diseases, including cardiovascular diseases, cerebrovascular diseases, as well as chronic kidney diseases, and discussed the advantages and limitations of their clinical applications.
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http://dx.doi.org/10.1038/s41392-022-01082-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272665PMC
July 2022

Effects of Plasma Exchange Combined with Immunoglobulin Therapy on Consciousness, Immune Function, and Prognosis in Patients with Myasthenia Gravis Crisis: A Prospective Randomized Test.

Comput Math Methods Med 2022 30;2022:7796833. Epub 2022 Jun 30.

Department of Neurology, The First Hospital of Changsha, Changsha, Hunan 410005, China.

Background: Myasthenia gravis (MG) is an acquired autoimmune disease. The main clinical features of MG are skeletal muscle fatigue and pathological fatigue, which worsen at night or after fatigue, such as dyspnea, dysphagia, and systemic weakness. Plasma exchange (PE) is often used in patients with acute exacerbation of MG. Intravenous immunoglobulin (IVIG) is a collection of immunoglobulins from thousands of donors. IVIG can replace a variety of immunosuppressants or PE. However, the effect of PE or IVIG on patients' consciousness, immune function, and prognosis is not clear.

Objective: A prospective randomized test of the effects of PE combined with immunoglobulin on consciousness, immune function, and prognosis in patients with myasthenia gravis crisis (MGC).

Methods: Sixty patients with MGC treated from February 2019 to April 2021 were enrolled in our hospital. The cases who received PE were set as the PE group, and those who received PE combined with immunoglobulin were set as the PE+immunoglobulin group. The efficacy, clinical score, state of consciousness, immune function, acetylcholine receptor antibody (AChR-Ab), lymphocyte (LYM), albumin (ALB) levels, and the incidence of adverse reactions were compared.

Results: The improvement rate was 100.005% in the treatment group and 83.33% in the PE group. After treatment, the clinical score of the PE+immunoglobulin group was lower than that of the PE group, and the clinical relative score of the PE+immunoglobulin group was higher than that of the PE group ( < 0.05). The number of conscious people in the PE+immunoglobulin group was more than that in the PE group ( < 0.05). Immunoglobulin A, immunoglobulin M, immunoglobulin G, and immunoglobulin G in the PE+immunoglobulin group were higher than those in the PE group ( < 0.05). The levels of AChR-Ab and ALB in the PE+immunoglobulin group were higher than those in the PE group, while the level of LYM in the PE+immunoglobulin group was lower than that in the PE group. The incidence of skin system, gastrointestinal system, nervous system, and systemic damage in the PE+immunoglobulin group was lower than that in the PE group ( < 0.05).

Conclusion: The treatment of MGC with PE combined with immunoglobulin can not only effectively enhance the consciousness and immune function of patients but also effectively promote the prognosis, and the safety of treatment can be guaranteed.
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http://dx.doi.org/10.1155/2022/7796833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262518PMC
July 2022

Facile Synthesis of Monodispersed Titanium Nitride Quantum Dots for Harmonic Mode-Locking Generation in an Ultrafast Fiber Laser.

Nanomaterials (Basel) 2022 Jul 1;12(13). Epub 2022 Jul 1.

Institute of Translational Medicine, First Affiliated Hospital (Shenzhen Second People's Hospital), Health Science Center, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.

As a member of the transition metal nitride material family, titanium nitride (TiN) quantum dots (QDs) have attracted great attention in optical and electronic fields because of their excellent optoelectronic properties and favorable stability. Herein, TiN QDs were synthesized and served as a saturable absorber (SA) for an ultrafast fiber laser. Due to the strong nonlinear optical absorption characteristics with a modulation depth of ~33%, the typical fundamental mode-locked pulses and harmonics mode-locked pulses can be easily obtained in an ultrafast erbium-doped fiber laser with a TiN-QD SA. In addition, at the maximum pump power, harmonic mode-locked pulses with a repetition rate of ~1 GHz (164th order) and a pulse duration of ~1.45 ps are achieved. As far as we know, the repetition rate is the highest in the ultrafast fiber laser using TiN QDs as an SA. Thus, these experimental results indicate that TiN QDs can be considered a promising material, showing more potential in the category of ultrafast laser and nonlinear optics.
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http://dx.doi.org/10.3390/nano12132280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268693PMC
July 2022

Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage through New Permeability Pathways.

Molecules 2022 Jun 29;27(13). Epub 2022 Jun 29.

Jiangsu Key Laboratory of Experimental and Translational Non-Coding RNA Research, School of Medicine, Yangzhou University, Yangzhou 225009, China.

To verify the size and emergence time of new permeability pathways (NPPs) in malaria parasites, the permeability of the -infected erythrocytes was tested with different particle sizes of nanomaterials by flow cytometry assay. The results confirmed the permeability of the host cell membrane increases with parasite maturation for the stage-development evolution of NPPs, and especially found that a particle size of about 50 nm had higher efficiency. As a kind of the novel nanomaterials, nitrogen-doped carbon dots (NCDs) showed no toxicity, specificity binding ability to the malaria parasites, and could label live elder blood-stage through NPPs, indicating the potential application in cell imaging. NPPs and some nanomaterials such as NCDs deserve more attention and exploration for the elimination and prevention of malaria.
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http://dx.doi.org/10.3390/molecules27134163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267939PMC
June 2022

The Use of Intergroup Social Comparison in Promoting Water Conservation: Evidence from a Survey Experiment in China.

Int J Environ Res Public Health 2022 Jun 24;19(13). Epub 2022 Jun 24.

Institute of Governance, Shandong University, Qingdao 266237, China.

This study examines the effect of the intergroup social comparison approach in promoting water conservation. In an online survey experiment, participants first encountered normative persuasive messages, informative persuasive messages, or intergroup social comparison messages and then reported their willingness to conserve water, prior water usage, and demographic information. Results showed a significantly higher willingness to conserve water in the intergroup social comparison condition compared to other conditions. We further investigated whether this social comparison effect was moderated by prior water usage and the strength of comparison. Results suggest that prior water usage, rather than the strength of comparison, moderated the influence of intergroup social comparison. Moreover, the moderating effect of prior water usage only works when participants receive a low level of strength of comparison.
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http://dx.doi.org/10.3390/ijerph19137749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265829PMC
June 2022

Research on Ecological Effect Assessment Method of Ecological Restoration of Open-Pit Coal Mines in Alpine Regions.

Int J Environ Res Public Health 2022 Jun 23;19(13). Epub 2022 Jun 23.

China IPPR International Engineering Co., Ltd., Beijing 100089, China.

Open-pit mining is an important form of coal mining in China, and its damage to the ecological environment is particularly obvious in alpine regions. The ecological restoration of alpine open-pit coal mines faces severe challenges, and its restoration effect will directly affect the ecological security of China. Meanwhile, comprehensive and system-oriented evaluation of ecological restoration effects is still insufficient in current research. In this study, we selected different quantities of assessment factors on the two scales of ecological project area and ecological impact area to evaluate the ecological restoration effect of an alpine open-pit coal mine. Then, we formed a multi-scale and multi-dimensional ecological restoration effect assessment model of the alpine open-pit coal mine and used this model to analyze the implementation effect of the ecological restoration project of the Baiyinhua No. 2 Open-pit Mine. The results show that the multi-scale and multi-dimensional ecological restoration effect assessment model of alpine open-pit coal mine proposed in this study can accurately characterize the restoration effect of open-pit coal mines in alpine regions and can also be used as a significant evaluation tool in the future ecological construction of mining areas. This study hopes the multi-scale and multi-dimensional ecological restoration effect assessment model of alpine open-pit coal mine can provide a comprehensive, systematic, and scientific evaluation method for the ecological restoration of alpine open-pit coal mines and provide a scientific basis for the ecological restoration and green development of relevant mining areas.
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http://dx.doi.org/10.3390/ijerph19137682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265715PMC
June 2022

LncRNA LINC00680 Acts as a Competing Endogenous RNA and Is Associated With the Severity of Myasthennia Gravis.

Front Neurol 2022 21;13:833062. Epub 2022 Jun 21.

Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Background And Purpose: Myasthenia gravis (MG) is a T cell-dependent antibody-mediated autoimmune disorder that can seriously affect patients' quality of life. However, few studies have focused on the severity of MG. Moreover, existing therapeutic efforts, including those targeting biomarkers for MG, remain unsatisfactory. Therefore, it is vital that we investigate the pathogenesis of MG and identify new biomarkers that can not only evaluate the severity of the disease but also serve as potential therapeutic targets. Long noncoding RNA LINC00680 has been found to be associated with the progression of a variety of diseases as a competing endogenous RNA (ceRNA). However, the specific role of LINC00680 in MG has yet to be clarified. Here, we aimed to investigate the association between LINC00680 and the severity of MG.

Methods: Bioinformatics tools, quantitative real-time PCR, Western blotting, and luciferase assays were selected to investigate key signaling pathways and RNA expression in patients with MG. The Quantitative MG Score scale and the MG Composite scale were used to evaluate the severity of MG in the included patients. Cell viability assays and flow cytometry analysis were selected to analyze cell proliferation and apoptosis.

Results: Compared with control subjects, the expression levels of LINC00680 and mitogen-activated protein kinase 1 (MAPK1) in peripheral blood mononuclear cells of patients with MG were both upregulated; the levels of miR-320a were downregulated. A positive correlation was detected between LINC00680 expression and the severity of MG. Luciferase reporter assays identified that LINC00680 acts as a target for miR-320a. The analysis confirmed that LINC00680 regulates the expression of MAPK1 by sponging miR-320a. Finally, the functional analysis indicated that LINC00680 promoted Jurkat cell proliferation and inhibited cellular apoptosis by sponging miR-320a.

Conclusion: LINC00680 may be associated with the severity of MG as a ceRNA by sponging miR-320a to upregulate MAPK1. These findings suggest that LINC00680 may represent a potential biomarker which evaluates the severity of MG and may serve as a therapeutic target.
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http://dx.doi.org/10.3389/fneur.2022.833062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253289PMC
June 2022

Exploration of the Cultivation Path of Medical Students' Politico-Ideological and Humanistic Quality Based on Deep Learning.

Comput Math Methods Med 2022 28;2022:5766675. Epub 2022 Jun 28.

College of Nursing, Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010059, China.

Medical talent development has its own characteristics, which means that political-ideological education in medical colleges must establish a development model that reflects these characteristics. In a methodological sense, research on the development of politico-ideological education in medical colleges should adhere to the coordinated development of politico-ideological education in medical colleges, which follows the requirements of the current situation and the three laws of politico-ideological education in medical colleges, namely, the requirements of the law of politico-ideological education, the law of teaching and educating people, and the law of study. In practice, in view of the practical contradictions in the politico-ideological work of medical colleges and universities at the present stage, explore the collaborative education mechanism of medical colleges and universities, constantly enrich the ways and methods of politico-ideological work in medical colleges and universities, improve the affinity, pertinence, and effectiveness of politico-ideological education, and constantly open up a new situation of politico-ideological education in colleges and universities. This work offers a path exploration approach based on deep learning for cultivating the political-ideological, and humanistic qualities of medical students' curriculum, and the model's performance is proven by simulation trials.
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http://dx.doi.org/10.1155/2022/5766675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256311PMC
July 2022
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