Publications by authors named "Shuang Li"

1,537 Publications

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Carboxylated and quaternized lignin enhanced enzymatic hydrolysis of lignocellulose treated by p-toluenesulfonic acid due to improving enzyme activity.

Bioresour Technol 2021 Oct 26;337:125465. Epub 2021 Jun 26.

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No. 2 Xuelin Road, Nanjing 210097, China. Electronic address:

Modificated lignins can affect enzymatic hydrolysis efficiency (EHE) because of changing physicochemical properties of lignin. In this study, carboxylated and quaternized lignin (CQL) and hydroxymethylated lignin (HML) were prepared to explore the effect of lignin modification on cellulase adsorption and EHE of p-toluenesulfonic acid treated corn stover (PCS). The results showed that CQL enhanced EHE of PCS due to the higher β-glucosidase (β-GL) activity, resulting from the formation of CQL-β-GL complexes with a lower binding free energy and the improvement of β-GL conformation made by the binding of CQL and β-GL. However, the drop in EHE due to the addition of HML was consequent on β-GL deactivation that was because the binding site of HML and β-GL overlapped with the carbohydrate binding domain of β-GL, causing the decrease in β-GL activity compared with CQL. This study would help deeply elucidate the effect of modified lignins on EHE and cellulase adsorption.
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http://dx.doi.org/10.1016/j.biortech.2021.125465DOI Listing
October 2021

Improved intestinal absorption and oral bioavailability of astaxanthin via poly (ethylene glycol)-graft-chitosan nanoparticle: preparation, in vitro evaluation and pharmacokinetics in rats.

J Sci Food Agric 2021 Jul 26. Epub 2021 Jul 26.

Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang, 212013, China.

Background: Astaxanthin (ASTA) is a kind of food-derived active ingredients (FDAI) with functions of antioxidant, antidiabetic, and nontoxicity, but its poor solubility and low bioavailability hinder further application in food industry. In this study, a novel carrier, polyethylene glycol grafted chitosan (PEG-g-CS), was applied in enhancing bioavailability of astaxanthin firstly. It encapsulated astaxanthin completely by solvent evaporation to manufacture ASTA-PEG-g-CS nanoparticle for improving absorption.

Results: The morphological characteristic of ASTA-PEG-g-CS nanoparticle showed individual and spherical, with a particle size below 200 nm and zeta potential about -26 mV. PEG-g-CS could encapsulate astaxanthin well by profiles of ATR-IR and XRD, and the encapsulated astaxanthin released fast in 15 min from in vitro release study. After rat single-pass intestinal perfusion study, low concentration of ASTA-PEG-g-CS nanoparticle (0.2 μg mL ^-1) had better absorption in intestine, especially jejunum could absorb most astaxanthin without change of concentration. Additionally, in vivo release study demonstrated that ASTA-PEG-g-CS nanoparticle enhanced oral bioavailability significantly.

Conclusion: This novel carrier, polyethylene glycol (PEG) grafted chitosan (PEG-g-CS), provided a simple way to encapsulate natural function food which improved the bioavailability of hydrophobic ingredients completely. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11435DOI Listing
July 2021

Circulating leptin and adiponectin levels in patients with pancreatic cancer.

Chin Med J (Engl) 2021 Jul 22. Epub 2021 Jul 22.

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China Department of Critical Care Medicine, Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210093, China Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.

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http://dx.doi.org/10.1097/CM9.0000000000001509DOI Listing
July 2021

Surfactin-methylene blue complex under LED illumination for antibacterial photodynamic therapy: Enhanced methylene blue transcellular accumulation assisted by surfactin.

Colloids Surf B Biointerfaces 2021 Jul 17;207:111974. Epub 2021 Jul 17.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, PR China. Electronic address:

Recently, increased attention has been focused on antibacterial photodynamic therapy (APDT) to treat multidrug-resistant bacterial infection due to the antibiotic abuse. Methylene blue has been used as a kind of efficient and cheap commercial photosensitizer in APDT. However, due to high hydrophilicity, methylene blue is not able to be transcellular intaken and accumulated efficiently. To promote accumulation and APDT efficiency of methylene blue, lipopeptide surfactin-methylene blue complex has been prepared through electrostatic interaction. The complex under LED irradiation was found to effectively reduce 5.0 Log CFU and 7.6 Log CFU for P. aeruginosa and S. aureus, respectively. The bacterial reduction efficiency is slightly higher than free methylene blue. The photosensitizers accumulation and APDT targeting protein have been characterized by fluorescence spectroscopy, fluorescence microscopy and protein electrophoresis techniques. These results demonstrated that more surfactin-methylene blue complex could be accumulated more into the cell, and inactivate bacteria through destroying intracellular protein under LED illumination. In comparison, free methylene blue under light could inactivate bacteria through destroying membrane protein and lipid structures. These results would provide valuable insight for developing advanced clinical medicine and designing photo-drug for photodynamic therapy.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111974DOI Listing
July 2021

The Receptor-Like Kinase Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density.

Int J Mol Sci 2021 Jul 6;22(14). Epub 2021 Jul 6.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, National Engineering Laboratory of Tree Breeding, Beijing Forestry University, Beijing 100083, China.

Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the gene, which is a member of the LRR-RLKs family from × ( × ), improves water use efficiency and enhances drought tolerance in triploid white poplar. localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24-34% lower and the stomatal size was 12-14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18-35% lower than the control line, and instantaneous water use efficiency was around 14-63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes trees promising candidates for establishing more water use efficient plantations.
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http://dx.doi.org/10.3390/ijms22147245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303786PMC
July 2021

FISH-Based Karyotype Analyses of Four Dracaena Species.

Cytogenet Genome Res 2021 Jul 21:1-6. Epub 2021 Jul 21.

Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of Medical Sciences & Peking Union Medical College, Jinghong, China.

The genus Dracaena is the main source of dragon's blood, which is a plant resin and has been used as traditional medicine since ancient times in different civilizations. However, the chromosome numbers and karyotypes present in this genus remain poorly understood. In this study, fluorescence in situ hybridization (FISH) using oligonucleotide probes for ribosomal DNAs (5S and 45S rDNA) and telomeric repeats (TTTAGGG)3 was applied to analyze 4 related species: Dracaena terniflora Roxb., Dracaena cambodiana Pierre ex Gagnep., Aizong (Dracaena sp.), and Dracaena cochinchinensis (Lour.) S.C. Chen. In all 4 species, both 5S and 45S rDNA showed hybridization signals in the paracentromeric region of a pair of chromosomes; the sizes of the 45S rDNA signals were larger than those of the 5S rDNA. Importantly, the telomeric repeat signals were located in the telomeric regions of almost all chromosomes. The results indicated that the chromosome number of all 4 Dracaena species is 2n = 40, and the lengths of the mitotic metaphase chromosomes range from 0.99 to 2.98 μm. Our results provide useful cytogenetic information, which will be beneficial to future studies in genome structure of the genus Dracaena.
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http://dx.doi.org/10.1159/000516897DOI Listing
July 2021

Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy.

ACS Sens 2021 Jul 20. Epub 2021 Jul 20.

State Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

Sensitive, selective, rapid, and label-free detection of pathogenic bacteria with high generality is of great importance for clinical diagnosis, biosecurity, and public health. However, most traditional approaches, such as microbial cultures, are time-consuming and laborious. To circumvent these problems, surface-enhanced Raman spectroscopy (SERS) appears to be a powerful technique to characterize bacteria at the single-cell level. Here, by SERS, we report a strategy for the rapid and specific detection of 22 strains of common pathogenic bacteria. A novel and high-quality silver nanorod SERS substrate, prepared by the facile interface self-assembly method, was utilized to acquire the chemical fingerprint information of pathogens with improved sensitivity. We also applied the mathematical analysis methods, such as the -test and receiver operating characteristic method, to determine the Raman features of these 22 strains and demonstrate the clear identification of most bacteria (20 strains) from the rest and also the reliability of this SERS sensor. This rapid and specific strategy for wide-range bacterial detection offers significant advantages over existing approaches and sets the base for automated and onsite detection of pathogenic bacteria in a complex real-life situation.
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http://dx.doi.org/10.1021/acssensors.1c00641DOI Listing
July 2021

Growth of Physicians and Nurse Practitioners Practicing Full Time in Nursing Homes.

J Am Med Dir Assoc 2021 Jul 15. Epub 2021 Jul 15.

Sealy Center on Aging, The University of Texas Medical Branch at Galveston, Galveston, TX, USA; Department of Medicine, The University of Texas Medical Branch at Galveston, Galveston, TX, USA; Department of Preventive Medicine and Population Health, The University of Texas Medical Branch at Galveston, Galveston, TX, USA.

Objectives: The objective was to describe the growth of physicians, nurse practitioners (NPs), and physician assistants (PAs) who practice full time in nursing homes, to assess resident and nursing home characteristics associated with receiving care from full-time providers, and describe variation among nursing homes in use of full-time providers.

Design: Retrospective cohort study.

Setting And Participants: A 20% national sample Medicare data on long-term care residents in 2008 to 2018 and the physicians, NPs, and PAs who submitted charges to Medicare for their care.

Methods: We measured the percentage of provider charges for services rendered in nursing homes, in addition to resident and facility characteristics.

Results: Full-time nursing home providers increased from 26.0% of all nursing home providers in 2008 to 44.6% in 2017. The largest increase was in NPs: from 1986 in 2008 to 4479 in 2017. Resident age, sex, Medicaid eligibility, and race/ethnicity had minimal association with the odds of having a full-time provider, whereas residents with an NP primary care provider were 23.0 times more likely (95% confidence interval = 21.6, 24.6) to have a full-time provider. Residents who received care from both a physician and an NP or PA increased from 33.6% in 2008 to 62.5% in 2018. There was large variation among facilities in the percentage of residents with full-time providers, from 5.72% of residents with full-time providers in the bottom quintile of facilities to 91.44% in the top quintile. Individual nursing homes accounted for 59% of the variation in whether a resident had a full-time provider.

Conclusions And Implications: The percentage of nursing home residents with full-time providers continues to grow, with very large variation among nursing homes.
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http://dx.doi.org/10.1016/j.jamda.2021.06.019DOI Listing
July 2021

Should we treat asymptomatic concurrent contralateral renal stones? A longitudinal analysis.

Urolithiasis 2021 Jul 16. Epub 2021 Jul 16.

Department of Urology, University of Wisconsin School of Medicine and Public Health, Medical Foundation Centennial Building, 1685 Highland Avenue, Madison, WI, 53705, USA.

The objective is to explore the need for future surgery among patients treated for asymptomatic concurrent contralateral stones versus those that were not. Upon IRB approval, we retrospectively reviewed records of patients who underwent stone surgeries (SWL, URS, PCNL) from 2009 to 2018. Patients were included if they were greater than 18 years old, had a minimum follow-up of 2 years, and had pre-operative imaging. Patients were divided into three groups: bilateral surgery, ipsilateral surgery with, and without asymptomatic concurrent contralateral stones. Cox regression was used to analyze patients' need for future surgery while controlling demographic and comorbid characteristics. Of the 1666 patients included, 51.9% were men. They were 59.7 ± 15 years and had a BMI of 31.3 ± 8.2 kg/m. During the follow-up of 5.2 ± 2.2 years (range 2-11 years), patients who had bilateral surgery and patients who had ipsilateral surgery without treatment of the asymptomatic concurrent contralateral stones had no difference in the need for future surgery (41.7% vs. 43%, p = 0.585). When stratified by stone size, patients with contralateral stones > 6 mm were more likely to require future surgical treatment than those treated bilaterally (p < 0.001). Our study demonstrates that treating asymptomatic concurrent contralateral stones does not lower the need for future surgical interventions. However, asymptomatic concurrent contralateral stones > 6 mm may portend earlier need for treatment. Therefore, bilateral treatment should be considered at presentation.
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http://dx.doi.org/10.1007/s00240-021-01281-3DOI Listing
July 2021

Effect of long working hours and insomnia on depressive symptoms among employees of Chinese internet companies.

BMC Public Health 2021 Jul 16;21(1):1408. Epub 2021 Jul 16.

National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Xicheng District, Beijing, P.R. China, 100050.

Background: In China, long working hours and insomnia are relatively common among internet company employees. Considering that both can affect mental health, we examined their independent and interaction effects on these employees' depressive symptoms (DS).

Methods: We analyzed data from the 2016 occupational health questionnaire survey conducted in 35 large-, medium-, and small-scale internet companies. Overall, 3589 full-time employees were recruited to evaluate the association among working hours, insomnia, and DS. The Patient Health Questionnaire (PHQ-9) was used to assess DS. The association of DS (PHQ-9 ≥ 10) with working hours (≤40, 41-50, 51-60, and > 60 h/week), insomnia (with or without), and interaction of both was estimated using multivariable logistic regression analysis.

Results: Compared with the group working for ≤40 h/week, the adjusted odds ratios (ORs) for DS among participants who worked for 41-50 h/week, 51-60 h/week, and > 60 h/week were 1.32 (1.11-1.56), 1.74 (1.35-2.24), and 2.54 (1.90-3.39), respectively. The ORs for DS among those with insomnia were 2.36 (2.04-2.74) after adjusting for general characteristics. The ORs for DS related to insomnia were similar [1.91 (1.46-2.50), 2.00 (1.61-2.50), respectively] in the participants who worked for < 50 h/week. However, among participants working for 51-60 h or > 60 h/week, the adjusted ORs for DS substantially increased to 4.62 (2.90-7.37) and 5.60 (3.36-9.33), respectively. Moreover, among the participants with insomnia, working overtime showed a greater association with DS.

Conclusions: We showed that long working hours and insomnia are independent factors associated with the prevalence of DS; furthermore, an interaction effect of long working hours and insomnia on DS was observed. For relieving DS in internet company employees, it is important to reduce insomnia.
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http://dx.doi.org/10.1186/s12889-021-11454-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283851PMC
July 2021

The complete chloroplast genome of (Zingiberaceae: Zingiberea) from Yunnan Province, China.

Mitochondrial DNA B Resour 2021 30;6(8):2114-2116. Epub 2021 Jun 30.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

is a medicinal plant endemic to Yunnan Province, China. In this study, its complete chloroplast genome was assembled and characterized. The total genome size of was 158,484 bp in length, consisting of a large single-copy region (LSC), a small single-copy region (SSC) and two inverted repeat regions (IRs) with 88,605 bp, 15,285 bp and 29,822 bp, respectively. Its GC content was 36.08%. The chloroplast genome encoded 113 unique genes, including 79 protein-coding, 30 tRNA, and four rRNA genes. The result of the phylogenetic analysis indicated that was related to var. and supported de Boer's classification that , , , , var. and belonged to the Clade.
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http://dx.doi.org/10.1080/23802359.2021.1938722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253217PMC
June 2021

Roles and Mechanisms of DNA Methylation in Vascular Aging and Related Diseases.

Front Cell Dev Biol 2021 28;9:699374. Epub 2021 Jun 28.

Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, China.

Vascular aging is a pivotal risk factor promoting vascular dysfunction, the development and progression of vascular aging-related diseases. The structure and function of endothelial cells (ECs), vascular smooth muscle cells (VSMCs), fibroblasts, and macrophages are disrupted during the aging process, causing vascular cell senescence as well as vascular dysfunction. DNA methylation, an epigenetic mechanism, involves the alteration of gene transcription without changing the DNA sequence. It is a dynamically reversible process modulated by methyltransferases and demethyltransferases. Emerging evidence reveals that DNA methylation is implicated in the vascular aging process and plays a central role in regulating vascular aging-related diseases. In this review, we seek to clarify the mechanisms of DNA methylation in modulating ECs, VSMCs, fibroblasts, and macrophages functions and primarily focus on the connection between DNA methylation and vascular aging-related diseases. Therefore, we represent many vascular aging-related genes which are modulated by DNA methylation. Besides, we concentrate on the potential clinical application of DNA methylation to serve as a reliable diagnostic tool and DNA methylation-based therapeutic drugs for vascular aging-related diseases.
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http://dx.doi.org/10.3389/fcell.2021.699374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273304PMC
June 2021

Alloyed AuPt nanoframes loaded on h-BN nanosheets as an ingenious ultrasensitive near-infrared photoelectrochemical biosensor for accurate monitoring glucose in human tears.

Biosens Bioelectron 2021 Jul 9;192:113490. Epub 2021 Jul 9.

School of Physics, Shandong University, Jinan, 250100, China. Electronic address:

Photo-electro-chemical (PEC) glucose biosensor has recently attracted extensive attention due to the double advantages of both photocatalysis via photon energy utilization and electrocatalytic oxidation through extra electric field. Compared with previous shorter wavelength (violet-visible) light-induced PEC reaction, the anticipated near infrared (NIR, >~700 nm) excited PEC biosensor with multiple fascinating features should be more suitable for clinical diagnostic biology. Herein, we report an ingenious NIR-PEC biosensor by loading alloyed AuPt nanoframes on two dimensional (2D) hexagonal boron nitride (h-BN) nanosheets. The obtained h-BN/AuPt nanoframes exhibit a remarkable higher NIR-PEC activity in comparison with other as-prepared h-BN/AuPt references. The improved PEC performance is attributed to the enhanced synergetic coupling effect between AuPt nanoalloys and constitutionally stable h-BN that gives rise to a stronger absorbance capacity and pronounced localized surface plasmon resonance (LSPR) in visible-NIR region as well as high free-electron mobility of framework-like Au/Pt. Interestingly, the obtained h-BN/AuPt nanoframes excited by 808 nm NIR light provide superior PEC accuracy and sensitivity as compared to visible or other NIR light irradiation. Then, the novel 808 nm NIR-PEC biosensor was used for precise glucose monitoring in human tears with a detectable concentration of 0.03~100 μM and a low detection limit of 0.406 nM. Undoubtedly, the proposed h-BN/AuPt nanoframes as an appealing NIR-PEC glucose biosensor can possess greater potential values for practical glucose monitoring in biomedicine.
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http://dx.doi.org/10.1016/j.bios.2021.113490DOI Listing
July 2021

A fluorescence-activatable tumor-reporting probe for precise photodynamic therapy.

J Mater Chem B 2021 Jul;9(29):5829-5836

State Key Laboratory for Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Innovation Center for Cell Signaling Network, Xiamen University, Xiamen 361005, China.

Approaches that could enable precise photodynamic therapy (PDT) are of therapeutic potential. We herein report a trifunctional probe (Glu-RdEB) that could be activated to generate fluorescent rhodamine species to pinpoint tumor foci. The probe contains a γ-glutaminyl moiety cleavable to γ-glutamyl transpeptidase (GGT) overexpressed in multiple tumors, an entity of an ENBS photosensitizer for PDT, and an entity of rhodamine fluorescently quenched by ENBS. Upon activation by tumor-associated GGT, the probe releases highly fluorescent rhodamine that is selectively confined in tumors whereby light irradiation leads to effective tumor regression in mice. These results indicate the feasibility of a fluorescently quenched dye-photosensitizer pair to yield tumor-activatable fluorescence to direct PDT.
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http://dx.doi.org/10.1039/d1tb00704aDOI Listing
July 2021

Higher FT4 level within the normal range predicts the outcome of cryoballoon ablation in paroxysmal atrial fibrillation patients without structural heart disease.

Ann Noninvasive Electrocardiol 2021 Jul 11:e12874. Epub 2021 Jul 11.

Nanjing Medical University, Nanjing, China.

Background: Accumulated evidence has indicated that a high-normal FT4 level is an independent risk factor for the clinical progression of AF. However, the association between elevated FT4 concentration within the normal range and AF recurrence after cryoballoon ablation in China is unknown.

Methods: This retrospective and observational study included 453 AF patients who underwent cryoballoon ablation from January 2016 to August 2018. Patients were classified into quartiles based on preprocedural serum FT4 concentration. The clinical characteristics of the patients and the long-term rate of AF recurrence after ablation were assessed.

Results: After a mean follow-up period of 17.4 ± 9.0 months, 91 (20.1%) patients suffered from AF recurrence. The AF recurrence rate by FT4 quartile was 17.7%, 19.0%, 21.4%, and 22.3% for participants with FT4 in quartile 1, 2, 3, and 4, respectively (p < .001). On multivariate Cox regression, FT4 concentration (HR: 1.187, 95% CI: 1.093-1.290, p < .001) and left atrial diameter (HR: 1.052, 95% CI: 1.014-1.092, p = .007) were significant predictors of AF recurrence. When stratifying for AF type, the rate of postoperative recurrence was independently increased as FT4 concentration increased in paroxysmal AF, but not in persistent AF (p < .001 in paroxysmal AF and p = .977 in persistent AF).

Conclusion: Higher FT4 level within the normal range predicted the outcome of cryoballoon ablation in Chinese paroxysmal AF patients without structural heart disease.
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http://dx.doi.org/10.1111/anec.12874DOI Listing
July 2021

Comparative Efficacy and Safety of TKIs Alone or in Combination With Antiangiogenic Agents in Advanced EGFR-Mutated NSCLC as the First-Line Treatment: A Systematic Review and Meta-Analysis.

Clin Lung Cancer 2021 Jun 13. Epub 2021 Jun 13.

Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. Electronic address:

Background: Several studies have suggested that patients with epidermal growth factor receptor (EGFR)-mutated non-small-cell lung cancer (NSCLC) might benefit from the use of tyrosine kinase inhibitors (TKIs) in combination with antiangiogenic agents. This study aimed to comprehensively review the available evidence regarding the efficacy and safety of first-line TKI plus antiangiogenic agents versus TKIs alone in EGFR-mutated advanced NSCLC.

Materials And Methods: A literature search was performed using PubMed to identify studies published up to Feb. 2020. Abstracts from major international conferences reported over the last 5 years were searched. The primary outcome was progression-free survival (PFS), and the secondary outcomes included overall survival (OS), the objective response rate (ORR), and toxicity.

Results: In total, 7 relevant trials comprising 1612 patients were identified. TKIs plus antiangiogenic agents led to significant improvements in PFS regardless of the EGFR mutation subtype and presence of brain metastasis. In particular, in the subgroup with the L858R mutation, the hazard ratio (HR) of PFS was 0.58 (95% confidence interval [CI], 0.48-0.71, P < .001). The OS following combined treatment was similar to that following TKI monotherapy. The ORR was increased with the use of TKIs plus antiangiogenic agents (HR 1.10, 95% CI, 1.01-1.20, P = .029). In the safety analyses, TKIs plus antiangiogenic agents exhibited a significantly increased incidence of adverse events of grade 3 or higher.

Conclusion: The use of TKIs plus antiangiogenic agents is associated with significantly improved PFS and ORR compared with TKIs alone in untreated EGFR-mutated NSCLC. The toxicities of combination therapy should be considered when making treatment choices.
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http://dx.doi.org/10.1016/j.cllc.2021.06.001DOI Listing
June 2021

A novel transgenic Cre allele to label mouse cardiac conduction system.

Dev Biol 2021 Jul 8;478:163-172. Epub 2021 Jul 8.

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA; Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX 77030, USA; Texas Heart Institute, Houston, TX 77030, USA. Electronic address:

The cardiac conduction system is a network of heterogeneous cell population that initiates and propagates electric excitations in the myocardium. Purkinje fibers, a network of specialized myocardial cells, comprise the distal end of the conduction system in the ventricles. The developmental origins of Purkinje fibers and their roles during cardiac physiology and arrhythmia have been reported. However, it is not clear if they play a role during ischemic injury and heart regeneration. Here we introduce a novel tamoxifen-inducible Cre allele that specifically labels a broad range of components in the cardiac conduction system while excludes other cardiac cell types and vital organs. Using this new allele, we investigated the cellular and molecular response of Purkinje fibers to myocardial injury. In a neonatal mouse myocardial infarction model, we observed significant increase in Purkinje cell number in regenerating myocardium. RNA-Seq analysis using laser-captured Purkinje fibers showed a unique transcriptomic response to myocardial infarction. Our finds suggest a novel role of cardiac Purkinje fibers in heart injury.
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http://dx.doi.org/10.1016/j.ydbio.2021.07.005DOI Listing
July 2021

Clinical Impact of the Institution of Moses Technology on Efficiency During Retrograde Ureteroscopy for Stone Disease: Single Center Experience.

J Endourol 2021 Jul 8. Epub 2021 Jul 8.

University of Wisconsin-Madison School of Medicine and Public Health, 5232, Madison, Wisconsin, United States;

Objective: To evaluate the clinical benefits of Moses technology compared to the regular mode with the Lumenis® Pulse™ P120H holmium laser during ureteroscopy for stone disease.

Patients And Methods: An IRB approved database of patients with urolithiasis was analyzed for ureteroscopies from 1/2020 - 12/2020 at an outpatient surgery center. Patients who underwent ureteroscopy with the Lumenis® Pulse™ P120H holmium laser system with the Moses or regular mode were included. Patient characteristics and stone parameters were collected. Operative room parameters were compared including procedural time, fragmentation/dusting time, lasing time and total energy used. Complication rates and stone free rates were also analyzed. Univariate analysis and MANCOVA controlling for cumulative stone size were performed. Patients with staged procedures were excluded.

Results: Of 197 surgical cases, 176 met inclusion criteria. Moses was utilized in 110 cases and regular mode in 66. There was no difference in cumulative stone size between Moses and regular modes (11.8±7.9 vs 11.6±9.2 mm, p=0.901). Procedural time (43.5±32.1 vs 39.8±24.6 min, p=0.436), fragmentation/dusting time (20.5±25.3 vs 17.1±16.1 min, p=0.430), lasing time (7.5±11.1 vs 6.7±7.9 min, p=0.570) and total energy used (5.1±6.7 vs 3.8±4.8 kJ, p=0.093) were also similar. Complications (6.4% vs 6.1%, p=0.936) and stone free rates (61.6% vs 73.5%, p=0.163) did not differ.

Conclusion: At our institution, Moses technology did not significantly change procedural time, fragmentation/dusting time, lasing time or total energy used. Moreover, there were no differences in complications or stone free rates. There may be technical benefits to Moses technology not captured in this analysis.
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http://dx.doi.org/10.1089/end.2021.0251DOI Listing
July 2021

Tumor invasive ability of papillary thyroid carcinomas is not conferred by acquired gene mutations.

J Investig Med 2021 Jul 7. Epub 2021 Jul 7.

Department of Ultrasound, First Affiliated Hospital of Dalian Medical University, Dalian, China

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The ability to predict whether a carcinoma would exhibit invasive ability in patients with PTC is important and has clinical implications for the selection of therapeutic strategies. Although several studies have focused on the genetic characterization of invasive cancer cells, the factors critical to the origination of invasive cancer cells are still unclear. This study aimed to determine whether genomic mutations contribute to the acquisition of the tumor invasion phenotype and to investigate the genetic features of invasive cancer cells in patients with PTC. We performed customized 48-gene deep exon sequencing in samples obtained from 88 patients with PTC via fine needle aspiration; the results revealed that no genetic changes were specifically associated with the tumor aggressiveness phenotype. Our results indicate that genetic mutations do not cause indolent PTCs to become invasive.
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http://dx.doi.org/10.1136/jim-2021-001971DOI Listing
July 2021

Development and validation of a novel N6-methyladenosine (m6A)-related multi- long non-coding RNA (lncRNA) prognostic signature in pancreatic adenocarcinoma.

Bioengineered 2021 Dec;12(1):2432-2448

Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, China.

Accumulating evidence has unveiled the pivotal roles of N6-methyladenosine (m6A) in pancreatic adenocarcinoma (PAAD). However, there are not many researches to predict the prognosis of PAAD using m6A-related long non-coding RNAs (lncRNAs). Raw data from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC), and the Genotype-Tissue Expression project (GTEx) were utilized to comprehensively analyze the expression and prognostic performances of 145 m6A-related lncRNAs in PAAD and to develop and validate a novel m6A-related multi-lncRNA prognostic signature (m6A-LPS) for PAAD patients. In total, 57 differentially expressed m6A-related lncRNAs with prognostic values were identified. Based on LASSO-Cox regression analysis, m6A-LPS was constructed and verified by using five-lncRNA expression profiles for TCGA and ICGC cohorts. PAAD patients were then divided into high- and low-risKBIE_A_1933868k subgroups with different clinical outcomes according to the median risk score; this was further verified by time-dependent receiver operating characteristic curves. Risk scores were significantly associated with clinical parameters such as histological grade and cancer status among PAAD patients. A nomogram consisting of risk score, grade, and cancer status was generated to predict the survival probability of PAAD patients, as also demonstrated by calibration curves. Discrepancies in cellular processes, signaling pathways, and immune status between the high- and low-risk subgroups were investigated by functional and single-sample gene set enrichment analyses. In conclusion, the novel m6A-LPS for PAAD patients was developed and validated, which might provide new insight into clinical decision-making and precision medicine.
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http://dx.doi.org/10.1080/21655979.2021.1933868DOI Listing
December 2021

Multi-angle head pose classification with masks based on color texture analysis and stack generalization.

Concurr Comput 2021 Apr 22:e6331. Epub 2021 Apr 22.

College of Robotics Beijing Union University Beijing China.

Head pose classification is an important part of the preprocessing process of face recognition, which can independently solve application problems related to multi-angle. But, due to the impact of the COVID-19 coronavirus pandemic, more and more people wear masks to protect themselves, which covering most areas of the face. This greatly affects the performance of head pose classification. Therefore, this article proposes a method to classify the head pose with wearing a mask. This method focuses on the information that is helpful for head pose classification. First, the H-channel image of the HSV color space is extracted through the conversion of the color space. Then use the line portrait to extract the contour lines of the face, and train the convolutional neural networks to extract features in combination with the grayscale image. Finally, stacked generalization technology is used to fuse the output of the three classifiers to obtain the final classification result. The results on the MAFA dataset show that compared with the current advanced algorithm, the accuracy of our method is 94.14% on the front, 86.58% on the more side, and 90.93% on the side, which has better performance.
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http://dx.doi.org/10.1002/cpe.6331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250277PMC
April 2021

Multi-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1.

PLoS Genet 2021 Jul 2;17(7):e1009640. Epub 2021 Jul 2.

Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.

Heterotrimeric G proteins were originally discovered through efforts to understand the effects of hormones, such as glucagon and epinephrine, on glucose metabolism. On the other hand, many cellular metabolites, including glucose, serve as ligands for G protein-coupled receptors. Here we investigate the consequences of glucose-mediated receptor signaling, and in particular the role of a Gα subunit Gpa2 and a non-canonical Gβ subunit, known as Asc1 in yeast and RACK1 in animals. Asc1/RACK1 is of particular interest because it has multiple, seemingly unrelated, functions in the cell. The existence of such "moonlighting" operations has complicated the determination of phenotype from genotype. Through a comparative analysis of individual gene deletion mutants, and by integrating transcriptomics and metabolomics measurements, we have determined the relative contributions of the Gα and Gβ protein subunits to glucose-initiated processes in yeast. We determined that Gpa2 is primarily involved in regulating carbohydrate metabolism while Asc1 is primarily involved in amino acid metabolism. Both proteins are involved in regulating purine metabolism. Of the two subunits, Gpa2 regulates a greater number of gene transcripts and was particularly important in determining the amplitude of response to glucose addition. We conclude that the two G protein subunits regulate distinct but complementary processes downstream of the glucose-sensing receptor, as well as processes that lead ultimately to changes in cell growth and metabolism.
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http://dx.doi.org/10.1371/journal.pgen.1009640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282090PMC
July 2021

[Determination of seven phenoxy acid herbicides in water by dispersive solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry based on cationic metal-organic framework mixed matrix membrane].

Se Pu 2021 Aug;39(8):896-904

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China.

Phenoxy acid herbicides are widely used because of their excellent efficiency and low cost. However, owing to their strong polarity and water solubility, these herbicides do not degrade easily in a water environment and persist for a long time in water bodies. These herbicides readily enter water bodies via surface runoff, infiltration, and other migration routes, thus affecting water quality safety. Therefore, it is of great significance to establish a sensitive and simple method for the quantitative analysis of phenoxy acid herbicides in environmental water. Given the low concentration of such contaminants in environmental water, appropriate detection methods are important. Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has high sensitivity and accuracy, thus being well suited for the phenoxy acid herbicides analysis. Sample preparation techniques are also important for the extraction and enrichment of contaminants in environmental water. Dispersive solid phase extraction (DSPE) has attracted considerable attention owing to its low cost, ease of operation, and low solvent consumption. In general, the selectivity and efficiency of solid phase extraction are largely dependent on the characteristics of the solid adsorbent materials. Ionic metal-organic frameworks (iMOFs) have excellent ion-exchange properties and show selective absorptivity to ionic compounds. In this work, a metal-organic framework (MOF) MIL-101-NH was synthesized by a facile hydrothermal method. Then, a cationic MOF mixed matrix membrane (MMM) was fabricated by soaking the MOFs in a polyvinylidene fluoride (PVDF) solution and further functionalization with quaternary amine groups. A method was developed for the determination of seven phenoxy acid herbicides in water by UPLC-MS/MS based on DSPE. The prepared material was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy to determine its functional groups and morphology. The results showed that there were quaternary amine groups in the material, and that the functionalization did not have any obvious effect on the chemical and crystal structures of MIL-101-NH. The prepared MIL-101-$NMe_{3}^{+}$-PVDF MMM was used as an adsorbent for DSPE to enrich the seven phenoxy acid herbicides in water. It is well known that the key factors influencing extraction efficiency are the adsorption and elution conditions. To establish the optimum extraction conditions, the influence of some important factors, including the adsorbent amount, sample pH, extraction time, elution solvent, elution volume, and elution time, was investigated in detail. Gradient elution was carried out with 0.01% formic acid aqueous solution and acetonitrile as the mobile phase. The target analytes were separated on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm), and multiple reaction monitoring (MRM) was conducted in the negative electrospray ionization mode. The external standard method was used for quantitative analysis. The established method was verified in terms of the linear ranges, limits of detection (LODs), limits of quantification (LOQs), recoveries, and precision. Under the optimal conditions, the seven phenoxy acid herbicides showed good linear relationships in their respective concentration ranges, and the correlation coefficients were all higher than 0.997. The LODs and LOQs were 0.00010-0.00090 μg/L and 0.00033-0.00300 μg/L, respectively. The recoveries were tested at three spiked levels of 0.005, 0.05, and 0.2 μg/L. The average recoveries of the seven compounds were in the range of 80% to 102%. The intra-day and inter-day relative standard deviations were within 1.4% to 9.4% and 4.2% to 12.6%, respectively. The established method was applied to the analysis of the phenoxy acid herbicides in tap water and reservoir water. Three levels of spiked samples were adopted to investigate the accuracy of the method. The results demonstrated that our method is applicable to the detection of trace phenoxy acid herbicides in water samples. In summary, this method has the advantages of simplicity, rapidity, and sensitivity, and it is suitable for the detection of the seven phenoxy acid herbicides in environmental water.
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http://dx.doi.org/10.3724/SP.J.1123.2021.01006DOI Listing
August 2021

Extracellular Granzyme K Modulates Angiogenesis by Regulating Soluble VEGFR1 Release From Endothelial Cells.

Front Oncol 2021 9;11:681967. Epub 2021 Jun 9.

Department of Pathology, University Medical Center Utrecht, Utrecht, Netherlands.

Angiogenesis is crucial for normal development and homeostasis, but also plays a role in many diseases including cardiovascular diseases, autoimmune diseases, and cancer. Granzymes are serine proteases stored in the granules of cytotoxic cells, and have predominantly been studied for their pro-apoptotic role upon delivery in target cells. A growing body of evidence is emerging that granzymes also display extracellular functions, which largely remain unknown. In the present study, we show that extracellular granzyme K (GrK) inhibits angiogenesis and triggers endothelial cells to release soluble VEGFR1 (sVEGFR1), a decoy receptor that inhibits angiogenesis by sequestering VEGF-A. GrK does not cleave off membrane-bound VEGFR1 from the cell surface, does not release potential sVEGFR1 storage pools from endothelial cells, and does not trigger sVEGFR1 release protease activating receptor-1 (PAR-1) activation. GrK induces sVEGFR1 mRNA and protein expression and subsequent release of sVEGFR1 from endothelial cells. GrK protein is detectable in human colorectal tumor tissue and its levels positively correlate with sVEGFR1 protein levels and negatively correlate with T4 intratumoral angiogenesis and tumor size. In conclusion, extracellular GrK can inhibit angiogenesis secretion of sVEGFR1 from endothelial cells, thereby sequestering VEGF-A and impairing VEGFR signaling. Our observation that GrK positively correlates with sVEGFR1 and negatively correlates with angiogenesis in colorectal cancer, suggest that the GrK-sVEGFR1-angiogenesis axis may be a valid target for development of novel anti-angiogenic therapies in cancer.
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http://dx.doi.org/10.3389/fonc.2021.681967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220216PMC
June 2021

Construction of lncRNA-Mediated ceRNA Network for Investigating Immune Pathogenesis of Ischemic Stroke.

Mol Neurobiol 2021 Jun 26. Epub 2021 Jun 26.

Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, Heilongjiang Province, China.

Ischemic stroke (IS) is a common and serious neurological disease. Extensive evidence indicates that activation of the immune system contributes significantly to the development of IS pathology. In recent years, some long non-coding RNAs (lncRNAs), acting as competing endogenous RNAs (ceRNAs), have been reported to affect IS process, especially the immunological response after stroke. However, the roles of lncRNA-mediated ceRNAs in immune pathogenesis of IS are not systemically investigated. In the present study, we generated a global immune-related ceRNA network containing immune-related genes (IRGs), miRNAs, and lncRNAs based on experimentally verified interactions. Further, we excavated an IS immune-related ceRNA (ISIRC) network through mapping significantly differentially expressed IRGs, miRNAs, and lncRNAs of patients with IS into the global network. We analyzed the topological properties of the two networks, respectively, and found that lncRNA NEAT1 and lncRNA KCNQ1OT1 played core roles in aforementioned two immune-related networks. Moreover, the results of functional enrichment analyses revealed that lncRNAs in the ISIRC network were mainly involved in several immune-related biological processes and pathways. Finally, we identified 17 lncRNAs which were highly related to the immune mechanism of IS through performing random walk with restart for the ISIRC network. Importantly, it has been confirmed that NEAT1, KCNQ1OT1, GAS5, and RMRP could regulate immuno-inflammatory response after stroke, such as production of inflammatory factors and activation of the immune cells. Our results suggested that lncRNAs exerted an important role in the immune pathogenesis of IS and provided a new strategy to do research on IS.
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http://dx.doi.org/10.1007/s12035-021-02426-6DOI Listing
June 2021

Deep learning-based automated left ventricular ejection fraction assessment using 2-D echocardiography.

Am J Physiol Heart Circ Physiol 2021 Aug 25;321(2):H390-H399. Epub 2021 Jun 25.

Laboratory of Cardiac Imaging and 3D Printing, Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong SAR, People's Republic of China.

Deep learning (DL) has been applied for automatic left ventricle (LV) ejection fraction (EF) measurement, but the diagnostic performance was rarely evaluated for various phenotypes of heart disease. This study aims to evaluate a new DL algorithm for automated LVEF measurement using two-dimensional echocardiography (2DE) images collected from three centers. The impact of three ultrasound machines and three phenotypes of heart diseases on the automatic LVEF measurement was evaluated. Using 36890 frames of 2DE from 340 patients, we developed a DL algorithm based on U-Net (DPS-Net) and the biplane Simpson's method was applied for LVEF calculation. Results showed a high performance in LV segmentation and LVEF measurement across phenotypes and echo systems by using DPS-Net. Good performance was obtained for LV segmentation when DPS-Net was tested on the CAMUS data set (Dice coefficient of 0.932 and 0.928 for ED and ES). Better performance of LV segmentation in study-wise evaluation was observed by comparing the DPS-Net v2 to the EchoNet-dynamic algorithm ( = 0.008). DPS-Net was associated with high correlations and good agreements for the LVEF measurement. High diagnostic performance was obtained that the area under receiver operator characteristic curve was 0.974, 0.948, 0.968, and 0.972 for normal hearts and disease phenotypes including atrial fibrillation, hypertrophic cardiomyopathy, dilated cardiomyopathy, respectively. High performance was obtained by using DPS-Net in LV detection and LVEF measurement for heart failure with several phenotypes. High performance was observed in a large-scale dataset, suggesting that the DPS-Net was highly adaptive across different echocardiographic systems. A new strategy of feature extraction and fusion could enhance the accuracy of automatic LVEF assessment based on multiview 2-D echocardiographic sequences. High diagnostic performance for the determination of heart failure was obtained by using DPS-Net in cases with different phenotypes of heart diseases. High performance for left ventricle segmentation was obtained by using DPS-Net, suggesting the potential for a wider range of application in the interpretation of 2DE images.
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http://dx.doi.org/10.1152/ajpheart.00416.2020DOI Listing
August 2021

Surface site density and utilization of platinum group metal (PGM)-free Fe-NC and FeNi-NC electrocatalysts for the oxygen reduction reaction.

Chem Sci 2020 Oct 13;12(1):384-396. Epub 2020 Oct 13.

The Electrochemical Catalysis, Energy and Materials Science Laboratory, Department of Chemistry, Technische Universität Berlin Straße des 17. 10623 Berlin Germany

Pyrolyzed iron-based platinum group metal (PGM)-free nitrogen-doped single site carbon catalysts (Fe-NC) are possible alternatives to platinum-based carbon catalysts for the oxygen reduction reaction (ORR). Bimetallic PGM-free MM-NC catalysts and their active sites, however, have been poorly studied to date. The present study explores the active accessible sites of mono- and bimetallic Fe-NC and FeNi-NC catalysts. Combining CO cryo chemisorption, X-ray absorption and Fe Mössbauer spectroscopy, we evaluate the number and chemical state of metal sites at the surface of the catalysts along with an estimate of their dispersion and utilization. Fe L-edge X-ray adsorption spectra, Mössbauer spectra and CO desorption all suggested an essentially identical nature of Fe sites in both monometallic Fe-NC and bimetallic FeNi-NC; however, Ni blocks the formation of active sites during the pyrolysis and thus causes a sharp reduction in the accessible metal site density, while with only a minor direct participation as a catalytic site in the final catalyst. We also use the site density utilization factor, , as a measure of the metal site dispersion in PGM-free ORR catalysts. enables a quantitative evaluation and comparison of distinct catalyst synthesis routes in terms of their ratio of accessible metal sites. It gives guidance for further optimization of the accessible site density of M-NC catalysts.
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http://dx.doi.org/10.1039/d0sc03280hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179675PMC
October 2020

Effects of lead exposure on the activation of microglia in mice fed with high-fat diets.

Environ Toxicol 2021 Jun 22. Epub 2021 Jun 22.

School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.

Lead (Pb) exposure can cause central nervous system (CNS) damage. The process of Pb neurotoxicity is accompanied by the microglia activation. In addition, microglia activation was observed under the intervention of high-fat diets (HFD). This study was designed to investigate the effect of Pb on the cognitive function of mice with HFD, with focus on the microglia activation in brain. Male C57BL/6J mice, 8 weeks of age, were randomly divided into control, HFD, Pb, and HFD + Pb groups. The results showed that HFD following Pb exposure could exacerbate the learning and memory impairment in mice. Pb exposure could promote microglia activation and increase the expression of M1 microglia marker and decrease the expression of M2 microglia marker in the hippocampus of mice with HFD. Our finding suggested that Pb exposure may aggravate CNS damage by promoting M1 polarization and inhibiting M2 polarization of hippocampal microglia in HFD mice.
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http://dx.doi.org/10.1002/tox.23312DOI Listing
June 2021

The rapeseed genotypes with contrasting NUE response discrepantly to varied provision of ammonium and nitrate by regulating photosynthesis, root morphology, nutritional status, and oxidative stress response.

Plant Physiol Biochem 2021 Jun 9;166:348-360. Epub 2021 Jun 9.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China; Microelement Research Center / Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs / State Environmental Protection Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment / College of Resources and Environment, Huazhong Agricultural University, 430070, Wuhan, China. Electronic address:

Ammonium (NH) and nitrate (NO) are the two predominant inorganic nitrogen (N) forms available to crops in agricultural soils. However, little is known about how the NH:NO ratio affect the growth of Brassica napus. Here, we investigated the impact of five NH:NO ratios (100:0, 75:25, 50:50, 25:75, 0:100) on plant growth, photosynthesis, root morphology, ammonium uptake, nutritional status, oxidative stress response, and relative expression of genes involved in these processes in two rapeseed genotypes with contrasting N use efficiency (NUE). Application of NO as a N source extremely improved rapeseed growth compare to NH. However, the best growth of the N-inefficient genotype was observed under 75:25 NH/NO ratio, while it happens for the N-efficient genotype only under the sole NO environment. The low-NUE genotype exhibited a more developed root system, higher photosynthetic capacity, higher nutrient accumulation, and better NH uptake ability under the 75:25 NH/NO ratio, resulting in a decrease of malondialdehyde (MDA) in root. However, the high-NUE genotype performed better in the above aspects under the NO-only condition. Nitrate decrease MDA by reducing the activities of superoxide dismutase, peroxidase, and catalase in root of the N-efficient genotype. Moreover, significant differences were detected for the expression levels of genes involved in N uptake and oxidative stress response between the two genotypes under two NH/NO ratios. Taken together, our results indicate that the N-inefficient rapeseed genotype prefers mixed supply of ammonium and nitrate, whereas the genotype with high NUE prefers sole nitrate environment.
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http://dx.doi.org/10.1016/j.plaphy.2021.06.001DOI Listing
June 2021

Correction to: Efficacy and safety of remimazolam besylate versus propofol during hysteroscopy: single-centre randomized controlled trial.

BMC Anesthesiol 2021 Jun 18;21(1):173. Epub 2021 Jun 18.

Department of Anaesthesiology, Mengcheng County No. 1 People's Hospital, Mengcheng, 233500, Anhui Province, P. R. China.

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http://dx.doi.org/10.1186/s12871-021-01390-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212503PMC
June 2021
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