Publications by authors named "Shuai Zhang"

1,156 Publications

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Reversine and herbal Xiang-Sha-Liu-Jun-Zi decoction ameliorate thioacetamide-induced hepatic injury by regulating the RelA/NF-κB/caspase signaling pathway.

Open Life Sci 2020 15;15(1):696-710. Epub 2020 Sep 15.

Department of Surgery, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong 518180, People's Republic of China.

This study investigated the anti-fibrotic effects of reversine and Chinese medicine Xiang-Sha-Liu-Jun-Zi decoction (XSLJZD) on thioacetamide (TAA)-induced hepatic injury. Sprague-Dawley rats were intraperitoneally administered with TAA, then injected with reversine intraperitoneally, and/or orally provided with XSLJZD. TAA resulted in liver injury with increases in the liver index and levels of serum aspartate aminotransferase (AST) and alanine aminotransferase. Reversine alleviated the liver index and AST level and improved TAA-induced pathological changes but decreased TAA-induced collagen deposition, and α-smooth muscle actin and transforming growth factor-β1 expression. Reversine also modulated the mRNA levels of inflammatory cytokines, such as RelA, interleukin (IL)-17A, IL-22, IL-1β, IL-6, NLR family pyrin domain containing 3, platelet-derived growth factor, and monocyte chemoattractant protein, and suppressed nuclear factor (NF)-κB (p65) phosphorylation and caspase 1 activation. Meanwhile, XSLJZD protected TAA-injured liver without increasing fibrosis and enhanced the regulating effect of reversine on RelA, IL-17A, IL-1β, and MCP-1 cytokines. In conclusion, reversine ameliorates liver injury and inhibits inflammation reaction by regulating NF-κB, and XSLJZD protects the liver through its synergistic effect with reversine on regulating inflammatory cytokines.
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http://dx.doi.org/10.1515/biol-2020-0059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747499PMC
September 2020

Analysis of Intestinal Mucosa Integrity and Gene Functions upon Porcine Epidemic Diarrhea Virus Infection in Pigs.

Animals (Basel) 2021 Mar 1;11(3). Epub 2021 Mar 1.

Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Porcine epidemic diarrhea virus (PEDV) infects intestinal epithelial cells, destroys the intestinal mucosal barrier and then causes diarrhea in piglets. Glucagon-like peptide-2 (GLP-2) is a specific intestinal growth hormone that promotes the repair of damaged intestinal mucosa and improves the intestinal barrier. In this study, we investigated the functions of porcine gene in regulating PEDV infection. The intestinal tissues with damaged intestinal structures caused by PEDV infection were first confirmed and collected. Expression analysis indicated that the gene was expressed in the duodenum, jejunum and ileum tissues, and the mRNA level was significantly down-regulated in jejunum and ileum of piglets with damaged intestinal mucosa. Infection of PEDV to porcine small intestinal epithelial cells in vitro showed that gene was significantly decreased, which was consistent with the expression pattern in intestinal tissues. In addition, we silenced the gene by shRNA interfering and found that the copy numbers of PEDV were remarkably increased in the gene silencing cells. Our findings suggest that the gene was potentially involved in regulating PEDV infection and in maintaining the integrity of the intestinal mucosal barrier structure, which could contribute to our understanding of the mechanisms of PEDV pathogenesis and provide a theoretical basis for the identification and application of resistant genes in pig selective breeding for porcine epidemic diarrhea.
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http://dx.doi.org/10.3390/ani11030644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000733PMC
March 2021

Differential Deep Convolutional Neural Network Model for Brain Tumor Classification.

Brain Sci 2021 Mar 10;11(3). Epub 2021 Mar 10.

State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, China.

The classification of brain tumors is a difficult task in the field of medical image analysis. Improving algorithms and machine learning technology helps radiologists to easily diagnose the tumor without surgical intervention. In recent years, deep learning techniques have made excellent progress in the field of medical image processing and analysis. However, there are many difficulties in classifying brain tumors using magnetic resonance imaging; first, the difficulty of brain structure and the intertwining of tissues in it; and secondly, the difficulty of classifying brain tumors due to the high density nature of the brain. We propose a differential deep convolutional neural network model (differential deep-CNN) to classify different types of brain tumor, including abnormal and normal magnetic resonance (MR) images. Using differential operators in the differential deep-CNN architecture, we derived the additional differential feature maps in the original CNN feature maps. The derivation process led to an improvement in the performance of the proposed approach in accordance with the results of the evaluation parameters used. The advantage of the differential deep-CNN model is an analysis of a pixel directional pattern of images using contrast calculations and its high ability to classify a large database of images with high accuracy and without technical problems. Therefore, the proposed approach gives an excellent overall performance. To test and train the performance of this model, we used a dataset consisting of 25,000 brain magnetic resonance imaging (MRI) images, which includes abnormal and normal images. The experimental results showed that the proposed model achieved an accuracy of 99.25%. This study demonstrates that the proposed differential deep-CNN model can be used to facilitate the automatic classification of brain tumors.
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http://dx.doi.org/10.3390/brainsci11030352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001442PMC
March 2021

Mitochondrial genome of Glover cucumber biotype (Hemiptera: Aphididae).

Mitochondrial DNA B Resour 2021 Mar 16;6(3):922-924. Epub 2021 Mar 16.

Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang, Henan, China.

The complete mitochondrial genome of Glover cucumber biotype was sequenced using traditional PCR amplification coupled with Sanger sequencing. The genome is 15,870 bp long, with 83.7% AT content (MW048625). The genome encodes 37 typical mitochondrial genes, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, a repeat region of 784 bp, and a control region of 627 bp. The base composition of the genome is A (45.4%), T (38.3%), C (10.5%), and G (5.8%). An analysis of two biotypes mitogenomes identified 77 single nucleotide polymorphisms and 1 insertion and deletion.
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http://dx.doi.org/10.1080/23802359.2021.1888328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971299PMC
March 2021

A bibliometric analysis of segmentectomy versus lobectomy for non-small cell lung cancer research (1992-2019).

Medicine (Baltimore) 2021 Apr;100(13):e25055

Department of Thoracic Surgery, The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital.

Background: This study intends to create a series of scientific maps to quantitatively estimate hot spots and emerging trends in segmentectomy versus lobectomy for non-small cell lung cancer (NSCLC) research with bibliometric methods.

Methods: Articles published on segmentectomy versus lobectomy for NSCLC were extracted from the Web of Science Core Collection (WoSCC). Extracted information was analyzed quantitatively using bibliometric analysis by CiteSpace to find hot spots and frontiers in this research area.

Results: A total of 362 scientific articles on segmentectomy versus lobectomy for NSCLC were collected, and the annual publication rate increased over time from 1992 to 2019. The leading country and the leading institution were the United States and University of Pittsburgh, respectively. Furthermore, the most prolific researchers were, namely, James D. Luketich, Rodney J. Landreneau, Matthew J. Schuchert, Morihito Okada, and David O. Wilson. The analysis of keywords pointed out that carcinoma, bronchogenic carcinoma, limited resection, segmental resection, and morbidity are hot spots and lymph node dissection, minimally invasive surgery, impact, epidemiology, and high risk are research frontiers in this field.

Conclusion: Publications related to segmentectomy versus lobectomy for NSCLC have made great achievements based on bibliometric analysis in recent years. However, further research and global collaboration are still required. Finally, we find that segmentectomy for the treatment of NSCLC is receiving much more attention from researchers globally compared with lobectomy in this research area.
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http://dx.doi.org/10.1097/MD.0000000000025055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021308PMC
April 2021

Muscle atrophy induced by overexpression of ALAS2 is related to muscle mitochondrial dysfunction.

Skelet Muscle 2021 Mar 30;11(1). Epub 2021 Mar 30.

Institute of Translational Medicine, National and Local Joint Engineering Laboratory of High-through Molecular Diagnostic Technology, the First People's Hospital of Chenzhou, The First Affiliated Hospital of Xiangnan University, Chenzhou, 423000, China.

Background: ALAS2 (delta-aminolevulinate synthase 2) is one of the two isoenzymes catalyzing the synthesis of delta-aminolevulinic acid (ALA), which is the first precursor of heme synthesis. ALAS2-overexpressing transgenic mice (Tg mice) showed syndrome of porphyria, a series of diseases related to the heme anabolism deficiency. Tg mice showed an obvious decrease in muscle size. Muscle atrophy results from a decrease in protein synthesis and an increase in protein degradation, which ultimately leads to a decrease in myofiber size due to loss of contractile proteins, organelles, nuclei, and cytoplasm.

Methods: The forelimb muscle grip strength of age-matched ALAS-2 transgenic mice (Tg mice) and wild-type mice (WT mice) were measured with an automated grip strength meter. The activities of serum LDH and CK-MB were measured by Modular DPP. The histology of skeletal muscle (quadriceps femoris and gastrocnemius) was observed by hematoxylin and eosin (HE) staining, immunohistochemistry, and transmission electron microscope. Real-time PCR was used to detect mtDNA content and UCP3 mRNA expression. Evans blue dye staining was used to detect the membrane damage of the muscle fiber. Single skeletal muscle fiber diameter was measured by single-fiber analyses. Muscle adenosine triphosphate (ATP) levels were detected by a luminometric assay with an ATP assay kit.

Results: Compared with WT mice, the strength of forelimb muscle and mass of gastrocnemius were decreased in Tg mice. The activities of serum CK-MB and LDH, the number of central nuclei fibers, and Evans blue positive fibers were more than those in WT mice, while the diameter of single fibers was smaller, which were associated with suppressed expression levels of MHC, myoD1, dystrophin, atrogin1, and MuRF1. Re-expression of eMyHC was only showed in the quadriceps of Tg mice, but not in WT mice. Muscle mitochondria in Tg mice showed dysfunction with descented ATP production and mtDNA content, downregulated UCP3 mRNA expression, and swelling of mitochondria.

Conclusion: ALAS2 overexpressing-transgenic mice (Tg mice) showed muscle dystrophy, which was associated with decreased atrogin-1 and MuRF-1, and closely related to mitochondrial dysfunction.
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http://dx.doi.org/10.1186/s13395-021-00263-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008657PMC
March 2021

Editorial: Material Surfaces and Interfaces at the Nanoscale: From Theory to Application.

Front Chem 2021 12;9:656661. Epub 2021 Mar 12.

Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.

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http://dx.doi.org/10.3389/fchem.2021.656661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994527PMC
March 2021

Generative adversarial network based data augmentation to improve cervical cell classification model.

Math Biosci Eng 2021 02;18(2):1740-1752

Endocrinology and Cardiovascular Disease Centre, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.

The survival rate of cervical cancer can be improved by the early screening. However, the screening is a heavy task for pathologists. Thus, automatic cervical cell classification model is proposed to assist pathologists in screening. In cervical cell classification, the number of abnormal cells is small, meanwhile, the ratio between the number of abnormal cells and the number of normal cells is small too. In order to deal with the small sample and class imbalance problem, a generative adversarial network (GAN) trained by images of abnormal cells is proposed to obtain the generated images of abnormal cells. Using both generated images and real images, a convolutional neural network (CNN) is trained. We design four experiments, including 1) training the CNN by under-sampled images of normal cells and the real images of abnormal cells, 2) pre-training the CNN by other dataset and fine-tuning it by real images of cells, 3) training the CNN by generated images of abnormal cells and the real images, 4) pre-training the CNN by generated images of abnormal cells and fine-tuning it by real images of cells. Comparing these experimental results, we find that 1) GAN generated images of abnormal cells can effectively solve the problem of small sample and class imbalance in cervical cell classification; 2) CNN model pre-trained by generated images and fine-tuned by real images achieves the best performance whose AUC value is 0.984.
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http://dx.doi.org/10.3934/mbe.2021090DOI Listing
February 2021

AhR/miR-23a-3p/PKCα axis contributes to memory deficits in ovariectomized and normal aging female mice.

Mol Ther Nucleic Acids 2021 Jun 18;24:79-91. Epub 2021 Feb 18.

Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province 150086, China.

The mechanism of estrogen deficiency-induced cognitive impairment is still not fully elucidated. In this study, we assessed the effect of microRNA (miRNA) on the memory of long-term estrogen-deficient mice after ovariectomy (OVX) and normal aging. We observed that 5-month OVX and 22-month-old normal aging female mice showed significantly impaired spatial and object recognition memory, declined hippocampal long-term potentiation (LTP), and decreased hippocampal protein kinase C α (PKCα) protein. Quantitative real-time PCR analysis showed upregulated miRNA-23a-3p (miR-23a-3p) in the hippocampus of 5-month OVX and 22-month-old female mice. , overexpression of miR-23a-3p downregulated PKCα by binding the 3¢ UTRs of mRNAs, which was prevented by its antisense oligonucleotide AMO-23a. , adeno-associated virus-mediated overexpression of miR-23a-3p (AAV-pre-miR-23a-3p) suppressed hippocampal PKCα and impaired the memory of mice. Chromatin immunoprecipitation analysis showed that aryl hydrocarbon receptor (AhR) binds the promoter region of miR-23a-3p. The AhR-dependent downregulation of PKCα could be prevented by AMO-23a as well. Furthermore, knockdown of miR-23a-3p using AAV-AMO-23a rescued the cognitive and electrophysiological impairments of OVX and normal aging female mice. We conclude that long-term estrogen deficiency impairs cognition and hippocampal LTP by activating the AhR/miR-23a-3p/PKCα axis. The knockdown of miR-23a-3p may be a potentially valuable therapeutic strategy for estrogen deficiency-induced memory deficits.
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http://dx.doi.org/10.1016/j.omtn.2021.02.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940705PMC
June 2021

MicroRNA-22 contributes to dexamethasone-induced osteoblast differentiation inhibition and dysfunction through targeting caveolin-3 expression in osteoblastic cells.

Exp Ther Med 2021 Apr 8;21(4):336. Epub 2021 Feb 8.

Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.

Osteoporosis is a common complication of long-term use of glucocorticoids (GCs) characterized by the loss of bone mass and damage of the microarchitecture as well as osteoblast dysfunction. Previous studies have demonstrated that microRNA-22 (miR-22) is the negative modulator of osteogenesis that may target caveolin-3 (CAV3), which has been reported to enhance bone formation and inhibit the progression of osteoporosis as well as apoptosis. The present study aimed to investigate whether miR-22 may be involved in dexamethasone (DEX)-induced inhibition of osteoblast differentiation and dysfunction by regulating CAV3 expression. Reverse transcription-quantitative PCR (RT-qPCR) was performed to measure the expression of miR-22 and western blotting was performed to determine protein levels. The results demonstrated that miR-22 expression was upregulated in DEX-treated osteoblastic cells compared with the control group. In addition, miR-22 mimic aggravated, whereas miR-22 inhibitor mitigated DEX-induced damage in osteoblastic cells compared with the control groups. Additionally, CAV3 was identified as the target of miR-22 in osteoblasts using RT-qPCR, western blotting and dual-luciferase reporter gene assay analysis. The results also demonstrated that silencing of CAV3 blocked the beneficial effects of miR-22 inhibitor against DEX-induced cell damage and apoptosis in osteoblasts, as evidenced by the increased expression levels of cleaved caspase-3, Bax and alkaline phosphatase activity as well as decreased cell viability and Bcl-2 levels. Collectively, these results indicate a novel molecular mechanism by which miR-22 contributes to DEX-induced osteoblast dysfunction and apoptosis via the miR-22/CAV3 pathway.
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http://dx.doi.org/10.3892/etm.2021.9767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903452PMC
April 2021

Experimental analysis of the measurement precision of spectral water-leaving radiance in different water types.

Opt Express 2021 Jan;29(2):2780-2797

The on-water radiometric approach employs a unique provision to obtain water-leaving radiance from nadir (Lw(λ)) which can be used for the calibration of ocean color satellites. In this effort, we address the measurement precision associated with Lw(λ) from a single on-water instrument, which is an important aspect of measurement uncertainty. First, we estimated the precision as the ratio of the standard deviation of the means of repeated measurements to the mean of these measurements. We show that the measurement precision for Lw(λ) is within 2.7-3.7% over 360-700 nm. The corresponding remote sensing reflectance spectra (Rrs(λ)) from the same instrument also exhibit a high precision of 1.9-2.8% in the same spectral domain. These measured precisions of radiance and reflectance over the 360-700 nm range are independent of the optical water type. Second, we quantified the consistency of on-water Lw(λ) and Rrs(λ) from two collocated systems for further insight into their measurement repeatability. The comparison reveals that Lw(λ) measurements in the 360-700 nm agree with each other with an absolute percentage difference of less than 3.5%. The corresponding Rrs(λ) data pairs are subjected to increased differences of up to 8.5%, partly due to variable irradiance measurements (Es(λ)). The evaluation of measurement precision corroborates the reliability of the on-water acquisition of radiometric data for supporting satellite calibration and validation.
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http://dx.doi.org/10.1364/OE.413784DOI Listing
January 2021

Rare-Earth Incorporated Alloy Catalysts: Synthesis, Properties, and Applications.

Adv Mater 2021 Mar 12:e2005988. Epub 2021 Mar 12.

Tianjin Key Lab for Rare Earth Materials and Applications, Center for Rare Earth and Inorganic Functional Materials, School of Materials Science and Engineering, National Institute for Advanced Materials, Nankai University, Tianjin, 300350, China.

To improve the performance of metallic catalysts, alloying provides an efficient methodology to design state-of-the-art materials. As emerging functional materials, rare-earth metal compounds can integrate the unique orbital structure and catalytic behavior of rare earth elements into metallic materials. Such rare-earth containing alloy catalysts proffer an opportunity to tailor electronic properties, tune charged carrier transport, and synergize surface reactivity, which are expected to significantly improve the performance and stability of catalysis. Despite its significance, there are only few reviews on rare earth containing alloys or related topics. This review summarizes the composition, synthesis, and applications of rare earth containing alloys in the field of catalysis. Subsequent to comprehensively summarizing and constructively discussing the existing work, the challenges and possibilities of future research on rare-earth metal compound materials are evaluated.
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http://dx.doi.org/10.1002/adma.202005988DOI Listing
March 2021

Non-antibiotic pharmaceuticals promote the transmission of multidrug resistance plasmids through intra- and intergenera conjugation.

ISME J 2021 Mar 10. Epub 2021 Mar 10.

Advanced Water Management Centre, The University of Queensland, St. Lucia, Brisbane, QLD, Australia.

Antibiotic resistance is a global threat to public health. The use of antibiotics at sub-inhibitory concentrations has been recognized as an important factor in disseminating antibiotic resistance via horizontal gene transfer. Although non-antibiotic, human-targeted pharmaceuticals are widely used by society (95% of the pharmaceuticals market), the potential contribution to the spread of antibiotic resistance is not clear. Here, we report that commonly consumed, non-antibiotic pharmaceuticals, including nonsteroidal anti-inflammatories (ibuprofen, naproxen, diclofenac), a lipid-lowering drug (gemfibrozil), and a β-blocker (propranolol), at clinically and environmentally relevant concentrations, significantly accelerated the dissemination of antibiotic resistance via plasmid-borne bacterial conjugation. Various indicators were used to study the bacterial response to these drugs, including monitoring reactive oxygen species (ROS) and cell membrane permeability by flow cytometry, cell arrangement, and whole-genome RNA and protein sequencing. Enhanced conjugation correlated well with increased production of ROS and cell membrane permeability. Additionally, these non-antibiotic pharmaceuticals induced responses similar to those detected when bacteria are exposed to antibiotics, such as inducing the SOS response and enhancing efflux pumps. The findings advance understanding of the transfer of antibiotic resistance genes, emphasizing the concern that non-antibiotic, human-targeted pharmaceuticals enhance the spread of antibiotic resistance among bacterial populations.
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http://dx.doi.org/10.1038/s41396-021-00945-7DOI Listing
March 2021

The role of IFT140 in early bone healing of tooth extraction sockets.

Oral Dis 2021 Mar 7. Epub 2021 Mar 7.

Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai, China.

Objectives: Primary cilium is a key organelle of regulating bone development and maintenance. The aim of this study is to investigate whether ciliary intraflagellar transporter protein 140 (IFT140) plays a positive role in extraction socket healing by promoting bone formation.

Materials And Methods: A left maxillary first molar extraction model was established using 6-week-old Ift140 (Ctrl group) and Ift140 , Osx-cre (cKO group) mice. The maxillary bone samples from 1, 2, and 3 weeks were postoperatively evaluated by micro-CT, molecular biology, and histomorphometry analysis. Alveolar bone marrow stromal cells (aBMSCs) from 4-week-old mice were cultured in vitro and tested for proliferation and osteogenic ability.

Results: Ciliated cells were predominantly observed in the early socket healing stage with highly expressed ciliary protein IFT140. Compared with the Ctrl group, the healing of extraction sockets in the cKO group was significantly delayed. The proliferation and osteogenic differentiation ability of aBMSCs were reduced in the cKO group.

Conclusion: IFT140 has a facilitating role in the early osteogenesis of extraction socket healing and is involved in regulating the proliferation and osteogenic differentiation of aBMSCs.
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http://dx.doi.org/10.1111/odi.13833DOI Listing
March 2021

Mechanism insight on drug skin delivery from polyurethane hydrogels: Roles of molecular mobility and intermolecular interaction.

Eur J Pharm Sci 2021 Mar 3;161:105783. Epub 2021 Mar 3.

Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning, 110016, China. Electronic address:

Though polyurethane (PU) hydrogel had great potential in topical drug delivery system, drug skin delivery behavior from hydrogel and the underlying molecular mechanism were still unclear. In this study, PU and Carbomer (CP as control) hydrogels were prepared with lidocaine (LID) and ofloxacin (OFX) as model drugs. In vitro skin permeation and tissue distribution study were conducted to evaluate the drug delivery behaviors. The underlying molecular mechanisms were characterized by drug release with octanol as release medium, rheological study, ATR-FTIR, NMR, and molecular simulation. The results showed that the skin permeation amount of LID-PU (45.50 ± 7.12 μg) was lower than LID-CP (45.50 ± 7.12 μg). And the LID diffusion coefficient of PU (26.21 μg/h) was also lower than CP (31.30 μg/h), which attributed to H-bonding between LID (-CONH) and PU (-NHCOO). However, the OFX-PU showed a higher skin permeation amount (10.06 ± 1.29 μg) than OFX-CP (5.28 ± 1.39 μg). And the OFX-PU also showed a higher diffusion coefficient (30.0 μg/h) than OFX-CP (21.37 μg/h), which was caused by increased mobility of hydrogel when interaction action site was C-O-C in PU. In conclusion, drug skin delivery behavior from PU hydrogel was controlled by molecular mobility and intermolecular interaction, which clarified the influence of the functional group of PU hydrogel on drug skin delivery behavior and broadened our understanding of PU hydrogel application in topical drug delivery system.
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http://dx.doi.org/10.1016/j.ejps.2021.105783DOI Listing
March 2021

Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1.

Mol Ther Nucleic Acids 2021 Mar 4;23:1191-1203. Epub 2021 Feb 4.

Department of Biomedical Materials Science, Third Military Medical University, Chongqing 400038, China.

Activated osteoclasts release large amounts of small extracellular vesicles (sEVs) during bone remodeling. However, little is known about whether osteoclast-derived sEVs affect surrounding cells. In this study, osteoclasts were generated by stimulating bone marrow macrophages (BMMs) with macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear actor κB ligand (RANKL). We performed microarray analysis of sEV-microRNAs (miRNAs)s secreted from osteoclast at different stages and identified four miRNAs that were highly expressed in mature osteoclast-derived sEVs. One of these miRNAs, miR-324, significantly induced osteogenic differentiation and mineralization of primary mesenchymal stem cells (MSCs) by targeting , a negative regulator of osteogenic differentiation. We next fabricated an sEV-modified scaffold by coating decalcified bone matrix (DBM) with osteoclast-derived sEVs, and the pro-osteogenic regeneration activities of the sEV-modified scaffold were validated in a mouse calvarial defect model. Notably, miR-324-enriched sEV-modified scaffold showed the highest capacity on bone regeneration, whereas inhibition of miR-324 in sEVs abrogated these effects. Taken together, our findings suggest that miR-324-contained sEVs released from mature osteoclast play an essential role in the regulation of osteogenic differentiation and potentially bridge the coupling between osteoclasts and MSCs.
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http://dx.doi.org/10.1016/j.omtn.2021.01.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900016PMC
March 2021

Long non-coding RNA TMPO-AS1 serves as a tumor promoter in pancreatic carcinoma by regulating miR-383-5p/SOX11.

Oncol Lett 2021 Apr 4;21(4):255. Epub 2021 Feb 4.

Department of General Surgery, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), Qingdao, Shandong 266000, P.R. China.

The dysregulation of lncRNA TMPO antisense RNA 1 (TMPO-AS1) has been detected in various malignant tumors. However, the role of lncRNA TMPO-AS1 remains unclear in pancreatic carcinoma. The present study aimed to elucidate the functional mechanism of TMPO-AS1 in pancreatic carcinoma. In the present study, RT-qPCR, western blotting, MTT, Transwell, luciferase reporter and xenograft assays were used to investigate the role of lncRNA TMPO-AS1 in pancreatic carcinoma. Upregulation of lncRNA TMPO-AS1 was revealed in pancreatic carcinoma tissues and cells. Furthermore, knockdown of TMPO-AS1 restrained cell proliferation and motility in pancreatic carcinoma. In addition, microRNA (miR)-383-5p acted as a 'sponge' for lncRNA TMPO-AS1. The expression levels of lncRNA TMPO-AS1 and miR-383-5p were mutually inhibited in pancreatic carcinoma. Moreover, miR-383-5p was revealed to directly target SRY-related high-mobility group box 11 (SOX11). Notably, SOX11 could promote the occurrence of pancreatic carcinoma by interacting with the lncRNA TMPO-AS1/miR-383-5p axis. In conclusion, upregulation of lncRNA TMPO-AS1 promoted tumor growth, cell migration and invasion in pancreatic carcinoma by downregulating miR-383-5p and upregulating SOX11.
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http://dx.doi.org/10.3892/ol.2021.12517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882873PMC
April 2021

Brain Tumor Detection and Classification by Hybrid CNN-DWA Model Using MR Images.

Curr Med Imaging 2021 Feb 23. Epub 2021 Feb 23.

Hebei University of Technology, State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Department of Biomedical Engineering, Tianjin 300130. China.

Objective: Medical image processing is an exciting research area. In this paper, we proposed new brain tumor detection and classification model using MR brain images to help the doctors in early detection and classification of the brain tumor with high performance and best Accuracy.

Materials: we trained and validated our model using five databases, including BRATS2012, BRATS2013, BRATS2014, BRATS2015, and ISLES-SISS 2015.

Methods: The advantage of the hybrid model proposed is its novelty that is used for the first time; our new method is based on a hybrid deep convolution neural network and deep watershed auto-encoder (CNN-DWA) model. The method consists of six phases, first phase is input MR images, second phase is preprocessing using filter and morphology operation, third phase is matrix that represents MR brain images, fourth is applying the hybrid CNN-DWA, fifth is brain tumor classification, and detection, while sixth phase is the performance of the model using five values.

Results And Conclusions: The novelty of our hybrid CNN-DWA model showed the best results and high performance with Accuracy around 98% and loss validation 0, 1. Hybrid model can classify and detect the Tumor clearly using MR images; comparing with other models like CNN, DNN, and DWA, we discover that the proposed model performs better than the above-mentioned models. Depending on the better performance of the proposed hybrid model, this helps in developing computer-aided system for early detection of brain tumors and helps the doctors to diagnose the patients better.
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http://dx.doi.org/10.2174/1573405617666210224113315DOI Listing
February 2021

Novel impedimetric sensing strategy for detecting ochratoxin A based on NH-MIL-101(Fe) metal-organic framework doped with cobalt phthalocyanine nanoparticles.

Food Chem 2021 Jul 18;351:129248. Epub 2021 Feb 18.

College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China. Electronic address:

Iron-based metal-organic framework, NH-MIL-101(Fe), was doped with different dosages of cobalt phthalocyanine nanoparticles (CoPc) to synthesize a series of NH-MIL-101(Fe)@CoPc nanocomposites. The NH-MIL-101(Fe)@CoPc nanocomposites were then employed to construct novel impedimetric aptasensors for the detection of ochratoxin A (OTA). Combining the intrinsic advantages of NH-MIL-101(Fe) (highly porous structure and excellently electrochemical activity) and CoPc (good physiochemical stability and strong bioaffinity), the NH-MIL-101(Fe)@CoPc nanocomposites show promising properties, which are beneficial for immobilizing OTA-targeted aptamer strands. Amongst, the developed impedimetric aptasensor based on NH-MIL-101(Fe)@CoPc, prepared using the mass ratio of NH-MIL-101(Fe):CoPc of 6:1, exhibits the best amplified electrochemical signal and highest sensitivity for detecting OTA. The detection limitation is 0.063 fg·mL within the OTA concentration of 0.0001-100 pg·mL, accompanying with high selectivity, good reproducibility and stability, acceptable regenerability, and wide applicability in diverse real samples. Consequently, the proposed sensing strategy can be applied for detecting OTA to cope with food safety.
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http://dx.doi.org/10.1016/j.foodchem.2021.129248DOI Listing
July 2021

Reversible engineering of topological insulator surface state conductivity through optical excitation.

Nanotechnology 2021 Feb 23;32(17):17LT01. Epub 2021 Feb 23.

National Laboratory of Solid State Microstructures, School of Physics and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, People's Republic of China.

Despite the broadband response, limited optical absorption at a particular wavelength hinders the development of optoelectronics based on Dirac fermions. Heterostructures of graphene and various semiconductors have been explored for this purpose, while non-ideal interfaces often limit the performance. The topological insulator (TI) is a natural hybrid system, with the surface states hosting high-mobility Dirac fermions and the small-bandgap semiconducting bulk state strongly absorbing light. In this work, we show a large photocurrent response from a field effect transistor device based on intrinsic TI Sn-BiSbTeS (Sn-BSTS). The photocurrent response is non-volatile and sensitively depends on the initial Fermi energy of the surface state, and it can be erased by controlling the gate voltage. Our observations can be explained with a remote photo-doping mechanism, in which the light excites the defects in the bulk and frees the localized carriers to the surface state. This photodoping modulates the surface state conductivity without compromising the mobility, and it also significantly modify the quantum Hall effect of the surface state. Our work thus illustrates a route to reversibly manipulate the surface states through optical excitation, shedding light into utilizing topological surface states for quantum optoelectronics.
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http://dx.doi.org/10.1088/1361-6528/abde01DOI Listing
February 2021

The association of Metabolic Syndrome and its Components with the Incidence and Survival of Colorectal Cancer: A Systematic Review and Meta-analysis.

Int J Biol Sci 2021 1;17(2):487-497. Epub 2021 Jan 1.

Department of General Surgery, Tianjin Union Medical Center, Tianjin 300121, China.

This meta-analysis was aimed to quantitatively assess the associations of metabolic syndrome (MetS) and its components with colorectal cancer (CRC). PubMed, EMBASE and Web of Science databases were systematically searched for eligible studies. A total of 18 studies for CRC incidence and 12 studies for CRC mortality were identified. MetS was associated with an increased risk of CRC incidence and mortality in male (RR: 1.28, 95 % CI 1.16-1.39, and 1.24, 1.18-1.31, respectively) and correlated with an increased risk of CRC incidence in female (RR: 1.21, 1.13-1.30), but not with CRC mortality in female. MetS increased the risk of cancer-specific mortality (RR: 1.72, 1.03-2.42), but not overall mortality. The risk estimates of CRC incidence changed little depending on age, sex, cancer site, the type of studies, ethnicity, publication year, or definition of MetS. As for CRC mortality, further stratified analyses indicated statistical significance in studies with assessing cancer-specific survival outcome, in male, a cohort design, ethnicity of non-Chinese or with definition of MetS as ≥ 3 metabolic abnormalities. Obesity and hyperglycemia are risk factors of CRC incidence in both male and female. Only dysglycemia is the risk factor for CRC mortality. MetS is associated with an increased risk of CRC incidence and cancer-specific mortality, but not overall mortality. In addition, MetS may increase the CRC mortality in male rather than in female. However, since most of the studies on CRC mortality were conducted in Chinese, further studies are needed to clarify this connection.
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http://dx.doi.org/10.7150/ijbs.52452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893592PMC
January 2021

Two-dimensional triazine-based porous framework as a novel metal-free bifunctional electrocatalyst for zinc-air batty.

J Colloid Interface Sci 2021 Jun 11;591:253-263. Epub 2021 Feb 11.

College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China. Electronic address:

A metal-free carbon catalyst, melem-cyanuric acid complex (MCAC), was prepared by hydrogen bonding assembly and further explored as a novel bifunctional electrocatalysts for oxygen evolution reaction (OER) and oxygen reduction reaction (ORR). The proposed MCAC network presented nanosheet-like structure, nitrogen-rich, and large specific surface area, which are close to the natures of graphitic carbon nitride (g-CN) and N-doped reduced graphene oxide (N-rGO), but giving much more defect active sites and regular framework structure. Compared with the g-CN, N-rGO and other reported carbon-nitride electrocatalysts, the MCAC nanosheets exhibited a lower overpotential of 1.45 V at a current density of 10 mA cm for OER, along with a higher half-potential of 0.8 V and larger limit current density of -6.0 mA cm for ORR. Density functional theory calculation revealed that the melem N atoms bonded with cyanurate greatly enhanced the OER activity by increasing the interaction between catalysts and intermediates. Furthermore, as a metal-free electrocatalyst, MCAC displayed superior reversible oxygen electrocatalytic activity, giving a small overpotential difference (0.76 V). The rechargeable zinc-air battery with MCAC as the air electrode in a two-electrode configuration showed a high open-circuit potential of 1.383 V and a specific capacity of 613.5 mA h g at 10 mA cm. This work opens up a new avenue to develop advanced porous solids as metal-free electrocatalysts for the energy storage and conversion applications.
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http://dx.doi.org/10.1016/j.jcis.2021.02.007DOI Listing
June 2021

AS602801 sensitizes glioma cells to temozolomide and vincristine by blocking gap junction communication between glioma cells and astrocytes.

J Cell Mol Med 2021 Feb 20. Epub 2021 Feb 20.

Department of Neurosurgery, ChangSha Central Hospital, Changsha, China.

Previous studies showed that the chemotherapeutic effect of temozolomide (TMZ) and vincristine (VCR) against glioma might be blunted by the co-culture with astrocytes, and connexin-43 (CX43) was thought to play a vital role in the communication between glioma cells and astrocytes. In this study, we aimed to investigate the combined chemotherapeutic effect of AS602801 and TMZ/ VCR in glioma cells both. Dye transfer assay was used to evaluate the gap junction activity between U251 cells and astrocytes. Western blot and immunohistochemistry were carried out to analyse the expression of p-JNK, CX43 and CASP-3 proteins treated under different conditions. AS602801 significantly suppressed the gap junction activity between U251 cells and astrocytes. The expression of p-JNK and CX43 was remarkably inhibited by AS602801. TMZ/VCR-induced apoptosis of glioma cells was effectively enhanced by AS602801 treatment. Accordingly, the inhibitory role of TMZ/VCR in the expression of p-JNK, CX43 and CASP-3 in glioma cells was notably restored by AS602801. Furthermore, in a glioma cell xenograft, AS602801 showed an apparent capability to enhance TMZ/VCR-induced tumour cell apoptosis through altering the expression of p-JNK, CX43 and CASP-3. The findings of this study demonstrated that the co-culture of glioma cells with astrocytes blunted the tumour killing effect of TMZ and VCR. AS602801 down-regulated CX43 expression by inhibiting JNK. And AS602801 also sensitized glioma cells to TMZ/VCR by blocking the gap junction communication between glioma cells and astrocytes via down-regulating CX43, indicating its potential role as a novel adjuvant chemotherapeutic agent in the treatment of glioma.
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http://dx.doi.org/10.1111/jcmm.16375DOI Listing
February 2021

Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.

Exp Ther Med 2021 Mar 21;21(3):238. Epub 2021 Jan 21.

Department of Orthopedics, General Hospital of Ningxia Medical University, Xingqing, Yinchuan, Ningxia 750004, P.R. China.

Glucocorticoid-induced osteoporosis is characterized by osteoblastic cell and microarchitecture dysfunction, as well as a loss of bone mass. Cell senescence contributes to the pathological process of osteoporosis and sodium hydrosulfide (NaHS) regulates the potent protective effects through delaying cell senescence. The aim of the present study was to investigate whether senescence could contribute to dexamethasone (Dex)-induced osteoblast impairment and to examine the effect of NaHS on Dex-induced cell senescence and damage. It was found that the levels of the senescence-associated markers, p53 and p21, were markedly increased in osteoblasts exposed to Dex. A p53 inhibitor reversed Dex-induced osteoblast injury, a process that was mitigated by NaHS administration through alleviating osteoblastic cell senescence. MicroRNA (miR)-22 blocked the impact of NaHS on Dex-induced osteoblast damage and senescence through targeting the regulation of Sirtuin 1 (sirt1) expression, as shown by the decreased cell viability and alkaline phosphatase activity, as well as an increased expression of p53 and p21. It was revealed that the sirt1 gene was the target of miR-22 in osteoblastic MC3T3-E1 cells through combining the results of dual luciferase reporter assays and reverse transcription-quantitative PCR, as well as western blot analyses. Silencing of sirt1 abolished the protective effect of NaHS against Dex-associated osteoblast senescence and injury. Taken together, the present study showed that NaHS prevents Dex-induced cell senescence and damage through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.
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http://dx.doi.org/10.3892/etm.2021.9669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851607PMC
March 2021

First-principles equation of state database for warm dense matter computation.

Phys Rev E 2021 Jan;103(1-1):013203

Department of Earth and Planetary Science, University of California, Berkeley, California 94720, USA.

We put together a first-principles equation of state (FPEOS) database for matter at extreme conditions by combining results from path integral Monte Carlo and density functional molecular dynamics simulations of the elements H, He, B, C, N, O, Ne, Na, Mg, Al, and Si as well as the compounds LiF, B_{4}C, BN, CH_{4}, CH_{2}, C_{2}H_{3}, CH, C_{2}H, MgO, and MgSiO_{3}. For all these materials, we provide the pressure and internal energy over a density-temperature range from ∼0.5 to 50 g cm^{-3} and from ∼10^{4} to 10^{9} K, which are based on ∼5000 different first-principles simulations. We compute isobars, adiabats, and shock Hugoniot curves in the regime of L- and K-shell ionization. Invoking the linear mixing approximation, we study the properties of mixtures at high density and temperature. We derive the Hugoniot curves for water and alumina as well as for carbon-oxygen, helium-neon, and CH-silicon mixtures. We predict the maximal shock compression ratios of H_{2}O, H_{2}O_{2}, Al_{2}O_{3}, CO, and CO_{2} to be 4.61, 4.64, 4.64, 4.89, and 4.83, respectively. Finally we use the FPEOS database to determine the points of maximum shock compression for all available binary mixtures. We identify mixtures that reach higher shock compression ratios than their end members. We discuss trends common to all mixtures in pressure-temperature and particle-shock velocity spaces. In the Supplemental Material, we provide all FPEOS tables as well as computer codes for interpolation, Hugoniot calculations, and plots of various thermodynamic functions.
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http://dx.doi.org/10.1103/PhysRevE.103.013203DOI Listing
January 2021

Image classification of osteoporotic vertebral fracture with endplate-disc complex Injury.

BMC Musculoskelet Disord 2021 Feb 17;22(1):197. Epub 2021 Feb 17.

Department of Orthopedics, The Affiliated Hospital of Southwest Medical University, NO. 25 Taiping S tr eet, Sichuan, 646000, Luzhou City, China.

Background: The purpose of this study was to use MRI and CT to observe osteoporosis vertebral fracture (OVF) combined with endplate-disc complex (EDC) injury and to classify the degree of EDC injury according to the changes in EDC signal intensity and morphology on the images.

Methods: We investigated the incidence of EDC injury, observed the morphology and signal intensity changes of EDC injury using MRI and CT, and graded the injuries from 0 to 4 according to their severity. We compared whether there were differences in the degree of EDC injury among different vertebral fractures, bone mineral density(BMD), and severity of vertebral fractures.

Results: A total of 479 patients were included in this study, of whom 321 had EDC injury adjacent to the fractured vertebral body. Among those, 158 cases were grade 0, 66 cases were grade 1, 72 cases were grade 2, 78 cases were grade 3, and 92 cases were grade 4. The degree of EDC injury associated with thoracolumbar vertebral fractures was more serious than that of EDC injuries associated with thoracic and lumbar vertebral body fractures. Vertebral fractures with severe osteoporosis were associated with more severe EDC injury. Additionally, the more severe the vertebral fracture, the more severe was the combined EDC injury.

Conclusion: This study found that the incidence rate of EDC injury reached 67.0%. Among patients with OVF, severe osteoporosis and severe fractures in the thoracolumbar segments were often associated with more severe EDC injury.
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http://dx.doi.org/10.1186/s12891-021-04070-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890829PMC
February 2021

Monoclinic dibismuth tetraoxide (m-BiO) for piezocatalysis: new use for neglected materials.

Chem Commun (Camb) 2021 Mar 17;57(22):2740-2743. Epub 2021 Feb 17.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CIC-AEET), School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China.

Piezocatalysis is a promising approach for environmental pollutant removal. Monoclinic dibismuth tetraoxide (m-BiO) was first applied to piezocatalyze organics under ultrasonic vibration. The built-in electric field with ultrasonic stress drives the separation of holes and electrons in m-BiO. Its excellent piezocatalytic activity, reusability and chemical stability make m-BiO a new candidate of piezocatalysis.
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http://dx.doi.org/10.1039/d0cc07064eDOI Listing
March 2021

Assessing right ventricular systolic function using ultrasonic speckle-tracking imaging in repaired Tetralogy of Fallot with different pulmonary artery branch angles.

Echocardiography 2021 Jan 3;38(1):89-96. Epub 2020 Dec 3.

Department of Echocardiography Diagnosis, Children's Hospital of Hebei Province, Hebei Medical University, Shijiazhuang, China.

Objective: This study assessed whether ultrasonic speckle-tracking imaging (STI) could help evaluate right ventricular systolic function in repaired Tetralogy of Fallot (TOF) with different pulmonary artery branch angles.

Methods: We retrospectively evaluated 64 patients who underwent surgery for TOF and 60 normal children. The angle between the left pulmonary artery and main pulmonary artery was measured using echocardiography and computed tomography angiography (CTA). Furthermore, STI was used to record the global longitudinal strain of the four-chamber view (GLS4), the global longitudinal strain of the two-chamber view (GLS2), and the global longitudinal strain of the right ventricle (RVGLS).

Results: The GLS4, GLS2, and RVGLS values in the TOF groups with different pulmonary artery branch angles were significantly lower than those in the control group. Furthermore, the GLS2 and RVGLS values were significantly lower for angles of 90-100° and <90° (vs >100°). Multivariate linear regression analyses revealed that pulmonary regurgitation and the angle between the left and main pulmonary arteries were two important factors affecting RVGLS. The Bland-Altman consistency test revealed good agreement regarding the pulmonary artery branch angles measured using echocardiography and CTA.

Conclusion: In patients with TOF, the RVGLS was lower for acute left pulmonary artery angulation than for round and blunt left pulmonary artery angulation. The angle of the pulmonary artery branches was an important factor affecting RVGLS. Echocardiography can be used to measure the angle of the pulmonary artery branches, which provides valuable information for surgical correction of pulmonary artery morphology.
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http://dx.doi.org/10.1111/echo.14948DOI Listing
January 2021

CXCR1 correlates to poor outcomes of EGFR-TKI against advanced non-small cell lung cancer by activating chemokine and JAK/STAT pathway.

Pulm Pharmacol Ther 2021 Apr 11;67:102001. Epub 2021 Feb 11.

The Third Department of Respiratory Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. Electronic address:

Objective: CXCR1, a member of the seven-transmembrane chemokine receptor family, promotes cell proliferation and metastasis in many tumors. The present study was undertaken to explore the interrelation between CXCR1 expression and the prognosis of advanced non-small cell lung cancer (NSCLC) in addition to the efficacy of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in lung adenocarcinoma (LUAD).

Methods: The expression of CXCR1 in NSCLC tissues was assessed by immunohistochemistry. The relationships between CXCR1 expression and clinical-pathological factors were investigated. Concomitantly, the relationship between CXCR1 expression and EGFR-TKI treatment efficacy was investigated. Gene set enrichment analysis (GSEA) was employed for the exploration of pathway enrichment, tumor immune estimation resource (TIMER) and gene expression profiling interactive analysis (GEPIA) for the inspection of the interrelationship between infiltration immune cells and CXCR1. After gain-and loss-of-function of CXCR1 in NSCLC cells, qRT-PCR and Western blot were applied to measure the levels of proteins associated with the chemokine pathway (CCL3 and CXCL2) and the JAK/STAT pathway (IL9R, PIAS4 and STAT5A).

Results: CXCR1 significantly correlated with poor prognosis of NSCLC patients. Additionally, CXCR1 limited the clinical efficacy of EGFR-TKIs in advanced LUAD (P = 0.029). In the tumor microenvironment, CXCR1 was positively associated with infiltration levels of immune markers in lung squamous cell carcinoma (LUSC) and LUAD. High expression of CXCR1 was implicated in the NOD-like receptor (NLR), cytokine/cytokine receptor, JAK/STAT and chemokine signaling pathways in LUAD and LUSC. Overexpression of CXCR1 in NSCLC cell lines enhanced expressions of CCL3, CXCL2, IL9R, PIAS4 and STAT5A, while knockdown of CXCR1 repressed expressions of CCL3, CXCL2, IL9R, PIAS4 and STAT5A.

Conclusion: CXCR1 is correlated with poor prognosis of NSCLC and affects the efficacy of EGFR-TKIs in LUAD.
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http://dx.doi.org/10.1016/j.pupt.2021.102001DOI Listing
April 2021

Practical microcircuits for handheld acoustofluidics.

Lab Chip 2021 Apr 10;21(7):1352-1363. Epub 2021 Feb 10.

Materials Science and Engineering Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. http://friend.ucsd.edu.

Acoustofluidics has promised to enable lab-on-a-chip and point-of-care devices in ways difficult to achieve using other methods. Piezoelectric ultrasonic transducers-as small as the chips they actuate-provide rapid fluid and suspended object transport. Acoustofluidic lab-on-chip devices offer a vast range of benefits in early disease identification and noninvasive drug delivery. However, their potential has long been undermined by the need for benchtop or rack-mount electronics. The piezoelectric ultrasonic transducers within require these equipment and thus acoustofluidic device implementation in a bedside setting has been limited. Here we detail a general process to enable the reader to produce battery or mains-powered microcircuits ideal for driving 1-300 MHz acoustic devices. We include the general design strategy for the circuit, the blocks that collectively define it, and suitable, specific choices for components to produce these blocks. We furthermore illustrate how to incorporate automated resonance finding and tracking, sensing and feedback, and built-in adjustability to accommodate devices' vastly different operating frequencies and powers in a single driver, including examples of fluid and particle manipulation typical of the needs in our discipline. With this in hand, the many groups active in lab-on-a-chip acoustofluidics can now finally deliver on the promise of handheld, point-of-care technologies.
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http://dx.doi.org/10.1039/d0lc01008aDOI Listing
April 2021