Publications by authors named "Shuai Wang"

1,716 Publications

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Adjuvant-free peptide vaccine targeting Clec9a on dendritic cells can induce robust antitumor immune response through Syk/IL-21 axis.

Theranostics 2021 24;11(15):7308-7321. Epub 2021 May 24.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.

Dendritic cells (DCs) can process the antigens of cancer vaccine and thus stimulate the CD8 T cells to recognize and kill the tumor cells that express these antigens. However, lack of promising carriers for presenting the antigens to DCs is one of the main barriers to the development of clinically effective cancer vaccines. Another limitation is the risk of inflammatory side effects induced by the adjuvants. It is still unclear how we can develop ideal adjuvant-free DC vaccine carriers without adjuvants. A 12-mer peptide carrier (CBP-12) with high affinity for Clec9a expressed on DCs was developed using an rational optimization method. The therapeutic effects of the adjuvant-free vaccine comprising CBP-12 and exogenous or endogenous antigenic peptides were investigated in terms of antigen cross-presentation efficacy, specific cytotoxic T lymphocyte response, and antitumor activity. We also explored the mechanism involved in the antitumor effects of the adjuvant-free CBP-12 vaccine. Finally, we assessed the effects of the CBP-12 conjugated peptide vaccine combined with radiotherapy. Here, we developed CBP-12 as a vaccine carrier that enhanced the uptake and cross-presentation of the antigens, thus inducing strong CD8 T cell responses and antitumor effects in both anti-PD-1-responsive (B16-OVA) and -resistant (B16) models, even in adjuvant-free conditions. CBP-12 bound to and activated Clec9a, thereby stimulating Clec9a DC to product IL-21, but not IL-12 by activating of Syk. The antitumor effects of the CBP-12 conjugated peptide vaccines could be blocked by an IL-21 neutralizing antibody. We also observed the synergistic antitumor effects of the CBP-12 conjugated peptide vaccine combined with radiotherapy. CBP-12 could serve as an adjuvant-free peptide vaccine carrier for cancer immunotherapy.
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http://dx.doi.org/10.7150/thno.56406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210616PMC
May 2021

[Comprehensive Treatment of An Advanced Lung Cancer Patient with 
EGFR Driver Gene Positive].

Zhongguo Fei Ai Za Zhi 2021 Jun;24(6):447-452

Department of Thoracic Surgery, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital), 
Beijing 100080, China.

Lung cancer is the most common malignant tumor and the leading cause of cancer-related death worldwide. Most of the patients have distant metastasis when visiting the doctor, which seriously affects the survival time and quality of life of the patients. With the development of molecular targeted drugs, lung cancer treatment has been transformed from traditional chemotherapy to targeted therapy and precision medicine has been gradually applied in clinical practice, which can make lung cancer patients live longer and have a better quality of life. We present a case of advanced lung cancer patient who presented to Department of Thoracic Surgery of Beijing Haidian Hospital five years ago. We chose the reasonable treatment options though the genetic tests and circulating tumor DNA tests. We summarized the adverse reactions in the whole course of treatment. The comprehensive therapy we utilized, including targeted therapy, chemotherapy, antiangiogenic agents and local radiotherapy, have resulted in our patient with remaining alive. For advanced non-small cell lung cancer with epidermal growth factor receptor (EGFR) mutation positive, individualized treatment was conducted based on precise genotyping and dynamic monitoring, which can not only control the tumor, but also have mild toxic and side effects. The survival time of the patients was prolonged and the quality of life was guaranteed.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.101.20DOI Listing
June 2021

Improving the performance of OLEDs by controlling the molecular orientation in charge carrier transport layers.

Opt Express 2021 May;29(11):16845-16856

The transition dipole moment (TDM) orientation in the emission layer (EML) of organic light-emitting diodes (OLEDs) have attracted increasing attention from many researchers. But the study point at the molecular orientation in the hole transport layer (HTL) and electron transport layer (ETL) was not reported widely. In this paper, the molecular orientation of HTLs and ETLs were controlled by the deposition rate. The angle-dependent PL spectra and the variable angle spectroscopic ellipsometry (VASE) were used for evaluating the molecular orientation of B3PYMPM and TAPC, respectively. We found that fast deposition rate can boost preferentially vertical molecular orientation in both molecules and facilitate the hole and electron mobility, which was tested by the current density-voltage and capacitance-voltage curves of HODs and EODs. Moreover, the HTLs and ETLs were employed in OLED devices to verify the influence of molecular orientation on charge carrier mobility, which determined the performance of OLEDs significantly.
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http://dx.doi.org/10.1364/OE.418566DOI Listing
May 2021

Surface illuminated interdigitated Ge-on-Si photodetector with high responsivity.

Opt Express 2021 May;29(11):16346-16361

To address the problem of traditional surface illuminated detectors being of low responsivity, this work proposes a large-size interdigitated "finger-type" germanium-on-silicon (Ge-on-Si) photodetector (PD) based on the surface illumination approach. For 1550 nm light with a surface incident power of -20 dBm at room temperature, the best responsivity of the PD achieved is ∼0.64 A/W at 0.5 V. At the same time, the optimal bandwidth reaches 1.537 MHz with 3.5 V applied voltage. In order to suppress the dark current induced noise, a Ge-on-Si avalanche photodiode (APD) with the interdigitated structure is designed. The avalanche voltage is designed ∼13.3 V at room temperature, and the dark current density in linear region is at mA/cm order. We believe this type of device can be applied in weak light detection condition.
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http://dx.doi.org/10.1364/OE.427343DOI Listing
May 2021

Vibration compensation method based on instantaneous ranging model for triangular FMCW ladar signals.

Opt Express 2021 May;29(11):15918-15939

Triangular frequency-modulated continuous-wave (FMCW) laser radars (ladars) are extremely sensitive to vibration errors. An FMCW ladar 3D imaging system may suffer from severe vibrations and can use only one-period echoes for the ranging of each observation spot; consequently, it can provide only few measurement results. These vibrations may cause large errors because conventional vibration compensation methods are ineffective when applied to fast disturbances with limited measurement results. To solve this problem, we analyze the influence of vibrations on FMCW ladar ranging and propose a vibration compensation method based on an instantaneous ranging model for one-period triangular FMCW ladar signals. We first use a synchrosqueezing wavelet transform to extract time-frequency curves of the up- and down-dechirp signals and then build an instantaneous ranging model that can characterize local vibration errors. Based on the instantaneous ranges, we remove the disturbance vibration errors by taking the mean values of the instantaneous ranges and obtain the target range by using the triangular relations of the up and down observations. Experiments based on synthetic and real data verify the effectiveness of the proposed method and its superiority over the three-point method and Doppler shift method in compensating for vibrations with different frequencies and noise levels.
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http://dx.doi.org/10.1364/OE.423289DOI Listing
May 2021

The Predictive Value of Lp(a) for Adverse Cardiovascular Event in ACS Patients With an Achieved LDL-C Target at Follow Up After PCI.

J Cardiovasc Transl Res 2021 Jun 21. Epub 2021 Jun 21.

Department of Cardiology, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

Low-density lipoprotein cholesterol (LDL-C) is a traditional and important risk factor for atherosclerotic cardiovascular disease (CVD). Recently, lipoprotein (a) (lp(a)) attracts considerable attention as a residual risk factor for CVD. However, the roles of lp(a) in acute coronary syndrome (ACS) patients with well-controlled LDL-C (≤1.8mmol/L) after percutaneous coronary intervention (PCI) remain unclear. Current study results demonstrated that occurrence of major adverse cardiovascular events (MACE) and recurrent myocardial infarction (MI) increased with the Lp(a) increasing in patients with LDL-C≤1.8mmol/L at 1-month follow-up. In relatively low-risk patients presented with ACS and underwent PCI (LDL-C ≤1.8mmol/L at 1-month follow-up), lp(a) is still independently related to adverse prognosis. Further researches of targeted therapy against lp(a) are warranted.
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http://dx.doi.org/10.1007/s12265-021-10148-2DOI Listing
June 2021

Molecular Traits and Functional Analysis of the CLAVATA3/Endosperm Surrounding Region-Related Small Signaling Peptides in Three Species of Genus.

Front Plant Sci 2021 4;12:671626. Epub 2021 Jun 4.

Research Base, Zhengzhou University, State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, China.

The CLAVATA3/endosperm surrounding region-related (CLE) small peptides are a group of C-terminally encoded and post-translationally modified signal molecules involved in regulating the growth and development of various plants. However, the function and evolution of these peptides have so far remained elusive in cotton. In this study, 55, 56, and 86 genes were identified in the , and genomes, respectively, and all members were divided into seven groups. These groups were distinctly different in their protein characteristics, gene structures, conserved motifs, and multiple sequence alignment. Whole genome or segmental duplications played a significant role in the expansion of the family in cotton, and experienced purifying selection during the long evolutionary process in cotton. is-acting regulatory elements and transcript profiling revealed that the genes of cotton exist in different tissues, developmental stages, and respond to abiotic stresses. Protein properties, structure prediction, protein interaction network prediction of GhCLE2, GhCLE33.2, and GhCLE28.1 peptides were, respectively, analyzed. In addition, the overexpression of , or in , respectively, resulted in a distinctive shrub-like dwarf plant, slightly purple leaves, large rosettes with large malformed leaves, and lack of reproductive growth. This study provides important insights into the evolution of cotton and delineates the functional conservatism and divergence of genes in the growth and development of cotton.
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http://dx.doi.org/10.3389/fpls.2021.671626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213210PMC
June 2021

Pontibaca salina sp. nov., isolated from marine sediment.

Arch Microbiol 2021 Jun 19. Epub 2021 Jun 19.

Marine College, Shandong University, Weihai, 264209, Shandong, People's Republic of China.

A Gram-stain-negative, oval or short rod-shaped, non-motile, aerobic bacterium, designated strain S1109L, was isolated from a marine sediment in Weihai, PR China. Cells were oxidase positive and catalase positive. Growth of strain S1109L occurred at 10-40 °C (optimum, 30-33 °C), pH 6.5-10.0 (optimum, 7.0-8.0) and in the presence of 1-21% (optimum, 4-6%) (w/v) NaCl. 16S rRNA gene sequence phylogeny indicated that strain S1109L was associated with the genus Pontibaca of the family Rhodobacteraceae because it showed the highest sequence similarity to Pontibaca methylaminivorans KCTC 22497 (97.5%). The average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) scores between strain S1109L and Pontibaca methylaminivorans KCTC 22497 were 74.6% and 18.7%. The major cellular fatty acids of strain S1109L were C cyclo ω8c and C ω7c. The polar lipids profiles of strain S1109L were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. Strain S1109L contained ubiquinone-10 as the major respiratory quinone. The genomic DNA G + C content was 55.9 mol%. On the basis of the evidence presented in this study, strain S1109L is considered to represent a novel species of the genus Pontibaca, for which the name Pontibaca salina sp. nov. is proposed. The type strain of is S1109L (= KCTC 82411 = MCCC 1H00441).
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http://dx.doi.org/10.1007/s00203-021-02434-zDOI Listing
June 2021

Sweat Gland Carcinoma of the Head and Neck: Case Report and Literature Review.

Ear Nose Throat J 2021 Jun 16:1455613211016717. Epub 2021 Jun 16.

School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.

Introduction: The main aim of this article is to discuss and summarize the research advancements and the treatment methods for sweat gland carcinoma (SGC) based on 2 cases of SGC in our hospital and the related literature.

Case Report: This article presents 2 patients with SGC who were treated in the China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases from 2007 to 2019. We analyzed the clinical features, therapies, and prognosis of the patients and searched for related literatures.

Discussion: Two patients underwent extended resection for local lesions with no adjuvant radiotherapy. Neither local recurrence nor distant metastasis was detected during follow-up. Reviewing previous literature, the treatment of SGC includes surgical resection, radiotherapy, and chemotherapy. We have not found an effective treatment. The prognosis of SGC occurred in head and neck is relatively good compared with another primary-site location, primary surgical excision with safe resection margins and neck dissection is recommended.
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http://dx.doi.org/10.1177/01455613211016717DOI Listing
June 2021

Kidney outcomes using a sustained ≥40% decline in eGFR: A meta-analysis of SGLT2 inhibitor trials.

Clin Cardiol 2021 Jun 15. Epub 2021 Jun 15.

Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Background: A recent meta-analysis of sodium-glucose cotransporter 2 (SGLT2) inhibitor outcome trials reported that SGLT2 inhibitors were associated with reduction in the risk of adverse composite kidney outcomes, with moderate heterogeneity across the trials; however, the endpoints were defined differently across the trials.

Hypothesis: The apparent heterogeneity of the meta-analysis of kidney composite outcomes of SGLT2 inhibitor trials will be substantially reduced by using a consistent assessment of sustained ≥40% decline in eGFR/chronic kidney dialysis/transplantation/renal death across trials.

Methods: We performed a meta-analysis of kidney composite outcomes from the four SGLT2 cardiovascular outcome trial programs conducted in general type 2 diabetes mellitus populations, which included, as a surrogate of progression to kidney failure, a sustained ≥40% decline in eGFR along with kidney replacement therapy and kidney death. The trials assessed were VERTIS CV (NCT01986881), CANVAS Program (NCT01032629 and NCT01989754), DECLARE-TIMI 58 (NCT01730534), and EMPA-REG OUTCOME (NCT01131676).

Results: Data from the trials comprised 42 516 individual participants; overall, 998 composite kidney events occurred. SGLT2 inhibition was associated with a significant reduction in the kidney composite endpoint (HR 0.58 [95% CI 0.51-0.65]) and with a highly consistent effect across the trials (Q statistic p = .64; I  = 0.0%).

Conclusions: Our meta-analysis highlights the value of using similarly defined endpoints across trials and supports the finding of consistent protection against kidney disease progression with SGLT2 inhibitors as a class in patients with type 2 diabetes mellitus who either have established atherosclerotic cardiovascular disease or are at high cardiovascular risk with multiple cardiovascular risk factors.
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http://dx.doi.org/10.1002/clc.23665DOI Listing
June 2021

Arterial Infusion Chemotherapy for Neoplastic Esophagogastric Anastomotic Strictures After Esophagectomy.

Front Oncol 2021 26;11:668593. Epub 2021 May 26.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Neoplastic esophagogastric anastomotic strictures after resection of esophageal cancer is a very difficult problem in clinical practice. We aim at to investigate the safety and feasibility of arterial infusion chemotherapy in treatment of neoplastic esophagogastric anastomotic strictures after esophagectomy.

Methods: From October 2014 to December 2019, 50 patients with Neoplastic esophagogastric anastomotic strictures after resection of esophageal cancer were assessed retrospectively. Preoperative dysphagia was grade III in 34 cases and grade IV in 16 cases. Thirty-eight patients had different degrees of dyspnea before surgery Twenty-five patients had intolerable (grade IV) dyspnea and airway stenting was undertaken before surgery. Thirteen patients had tolerable dyspnea that did not require airway stenting, and preoperative dyspnea was grade III.

Results: All patients were successfully treated with arterial infusion chemotherapy, no paraplegia or death occurred. The dysphagia grade of 50 patients after AIC was compared: one case had grade I, 40 cases had grade II, and nine cases had grade III. Thirteen patients had tolerable dyspnea that did not necessitate airway stenting. Dyspnea was classified as grade I in five cases and grade II in eight cases. After 1-3 courses of AIC, 50 patients were followed up for a complete response (eight cases), partial response (28) and stable disease (14 cases). Total objective effective rate (complete response+ partial response) and disease control rate(complete response + partial response + stable disease)were 72.0% and 100.0%, respectively. The median duration of follow-up was 8.5 months. One-year survival was 46.0%.

Conclusion: Arterial infusion chemotherapy is safe and efficacious treatment for Neoplastic esophagogastric anastomotic strictures after esophagectomy.
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http://dx.doi.org/10.3389/fonc.2021.668593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187754PMC
May 2021

High-Performance Joule Heating and Electromagnetic Shielding Properties of Anisotropic Carbon Scaffolds.

ACS Appl Mater Interfaces 2021 Jun 11;13(24):29101-29112. Epub 2021 Jun 11.

Laboratory of Advanced Materials, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, P. R. China.

Highly efficient electrical heaters along with excellent electromagnetic interference (EMI) shielding properties are urgently required for the progress of miniaturization electronics, artificial intelligence, and smart heating management setups. Herein, lignin removal, which comprises two efficient and versatile steps, followed by carbonization produces multifunctional carbon monoliths derived from natural wood. The obtained carbonized wood exhibits a high specific surface area (655.14 m/g) and electrical (17.5 S/cm) and thermal conductivity (0.58 W/m·K), superhydrophilicity (contact angle of ∼0°), and excellent EMI shielding ability and Joule heating performance. The high electrical conductivity renders a low-voltage-actuated Joule heating performance and fascinating EMI shielding effectiveness of 55 dB, primarily resulting from the absorption mechanism. Moreover, regulation of the carbonized woods derived from the longitudinal to the radial direction enables transformation of hydrophilicity, strong thermal conductivity, and absorption-dominated EMI shielding to hydrophobicity, thermal insulation, and reflection-dominated EMI shielding. This is attributed to the unique anisotropic microstructure of carbon scaffolds. It is believed that these multifunctional carbon scaffolds can be used for intelligent electronics, EMI shielding, and thermal heating instruments.
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http://dx.doi.org/10.1021/acsami.1c05327DOI Listing
June 2021

Molecular Identification and Genetic Characterization of Early-Stage Multiple Primary Lung Cancer by Large-Panel Next-Generation Sequencing Analysis.

Front Oncol 2021 24;11:653988. Epub 2021 May 24.

Department of Thoracic Surgery, Beijing Haidian Hospital, Beijing, China.

Objective: The incidence of early stage multiple primary lung cancer (MPLC) has been increasing in recent years, while the ideal strategy for its diagnosis and treatment remains controversial. The present study conducted genomic analysis to identify a new molecular classification method for accurately predicting the diagnosis and therapy for patients with early stage MPLC.

Methods: A total of 240 tissue samples from 203 patients with multiple-non-small-cell lung cancers (NSCLCs) (n = 30), early stage single-NSCLC (Group A, n = 94), and advanced-stage NSCLC (Group B, n = 79) were subjected to targeted multigene panel sequencing.

Results: Thirty patients for whom next-generation sequencing was performed on >1 tumor were identified, yielding 45 tumor pairs. The frequencies of , and mutations exhibited significant differences between early and advanced-stage NSCLCs. The prevalence of the mutation in early stage NSCLC was remarkably higher than that in advanced-stage NSCLC ( = 0.047). The molecular method classified tumor pairs into 26 definite MPLC tumors and four intrapulmonary metastasis (IM) tumors. A high rate of discordance in driver genetic alterations was found in the different tumor lesions of MPLC patients. The prospective Martini histologic prediction of MPLC was discordant with the molecular method for three patients (16.7%), particularly in the prediction of IM (91.7% discordant).

Conclusions: Comprehensive molecular evaluation allows the unambiguous delineation of clonal relationships among tumors. In comparison, the Martini and Melamed criteria have notable limitations in the recognition of IM. Our results support the adoption of a large panel to supplement histology for strongly discriminating NSCLC clonal relationships in clinical practice.
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http://dx.doi.org/10.3389/fonc.2021.653988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183821PMC
May 2021

CHOP Regulates Endoplasmic Reticulum Stress-Mediated Hepatoxicity Induced by Monocrotaline.

Front Pharmacol 2021 24;12:685895. Epub 2021 May 24.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Monocrotaline (MCT), a pyrrolizidine alkaloid, is the major toxin in , which causes cell apoptosis in humans and animals. It has been reported that the liver is a vulnerable target of MCT. However, the exact molecular mechanism of the interaction between endoplasmic reticulum (ER) stress and liver injury induced by MCT is still unclear. In this study, the cytotoxicity of MCT on primary rat hepatocytes was analyzed by a CCK-8 assay and Annexin V-FITC/PI assay. Protein expression was detected by western blotting and immunofluorescence staining. As a result, MCT significantly decreased the cell viability and mediated the apoptosis of primary rat hepatocytes. Meanwhile, MCT could also induce ER stress in hepatocytes, indicated by the expression of ER stress-related proteins, including GRP78, p-IRE1α, ATF6, p-eIF2α, ATF4, and CHOP. Pretreatment with 4-PBA, an inhibitor of ER stress, or knockdown of CHOP by siRNA could partly enhance cell viability and relieve the apoptosis. Our findings indicate that ER stress is involved in the hepatotoxicity induced by MCT, and CHOP plays an important role in this process.
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http://dx.doi.org/10.3389/fphar.2021.685895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181757PMC
May 2021

Pharmacokinetics-Based Chronoefficacy of and Tripterygium Glycoside Tablet Against Rheumatoid Arthritis.

Front Pharmacol 2021 24;12:673263. Epub 2021 May 24.

Institute of Molecular Rhythm and Metabolism, Guangzhou University of Chinese Medicine, Guangzhou, China.

Rheumatoid arthritis is a systemic autoimmune disease characterized by synovial inflammation and bone destruction. Identifying drugs with time-varying efficacy and toxicity, and elucidating the mechanisms would help to improve treatment efficacy and reduce adverse effects. Here, we aimed to determine the chronoefficacy of (SS) and tripterygium glycoside tablet (TGT) against rheumatoid arthritis in mice, and to investigate a potential role of circadian pharmacokinetics in generating chronoefficacy. SS extract and TGT suspension were prepared with ultrasonication. Effects of SS and TGT on collagen-induced arthritis (CIA) were evaluated by measuring TNF-α and IL-6 levels. SS dosed at ZT18 was more effective in protecting against CIA than drug dosed at ZT6 (i.e., lower levels of key inflammatory factors at ZT18 than at ZT6). This was accompanied by higher systemic exposure levels of strychnine and brucine (two main putative active ingredients of SS) in ZT18-treated than in ZT6-treated CIA mice. TGT dosing at ZT2 showed a better efficacy against CIA as compared to herb doing at ZT14. Consistently, ZT2 dosing generated a higher exposure of triptolide (a main putative active ingredient of TGT) as compared to ZT14 dosing in CIA mice. Moreover, strychnine, brucine, and triptolide significantly inhibited the proliferation of fibroblast-like synoviocytes, and reduced the production of TNF-α and IL-6 and the mRNAs of TNF-α, IL-6, COX-2, and iNOS, suggesting that they possessed an anti-arthritis activity. In conclusion, SS and TGT display chronoefficacy against rheumatoid arthritis in mice, that is attributed to circadian pharmacokinetics of main active ingredients. Our findings have implications for improving treatment outcomes of SS and TGT timed delivery.
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http://dx.doi.org/10.3389/fphar.2021.673263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181759PMC
May 2021

Safety and immunogenicity of a quadrivalent inactivated subunit non-adjuvanted influenza vaccine: A randomized, double-blind, active-controlled phase 1 clinical trial.

Vaccine 2021 Jun 1;39(29):3871-3878. Epub 2021 Jun 1.

Department of Research and Development, Ab&B Biotech Co., Ltd, Taizhou, Jiangsu, China; Department of Research and Development, Yither Biotech Co., Ltd, Shanghai, China. Electronic address:

Quadrivalent influenza inactivated vaccine (IIV4) is more likely to provide wider protection against yearly circulating influenza viruses than trivalent inactivated influenza vaccine (IIV3). In this study, a total of 320 participants were allocated to four age cohorts (6-35 months, 3-8 years, 9-17 years, and ≥ 18 years; 80 participants/cohort) according to their actual date of birth. Participants in each cohort were randomly assigned to two groups to receive intramuscular injection of the trial vaccine or the comparative vaccine in a one-dose (3-8 years, 9-17 years,and ≥ 18 years) schedule on day 0 or two-dose (6-35 months cohort) schedule on day 0 and 28. The first objective is to evaluate the safety and immunogenicity of the full-dose subunit non-adjuvanted IIV4 (FD-subunit NAIIV4) we developed versus an active-control, China-licensed split-virion NAIIV4, in people ≥ 3 years. The second objective is to evaluate the safety and immunogenicity of FD-subunit NAIIV4 versus the half-dose (HD-subunit NAIIV4) in toddlers aged 6-35 months. Results showed that all adverse reactions noted were rare, mild, and self-limited. In ≥ 3 years cohorts, systemic adverse reactions in FD-subunit NAIIV4 groups were less than the active control split-virion NAIIV4 groups ([Systemic adverse reaction rates (95%CI)], 15.0 (8.6-21.4) versus 19.2(12.1-26.2), p = 0.391). The overall seroprotection efficacy after vaccination were comparable between FD-subunit NAIIV4 and the active control split-virion NAIIV4([Seroprotection rates (95%CI)], H1N1, 99.2(81.3-100.0) versus 94.9(90.9-98.9), p = 0.117; H3N2, 81.7(74.7-88.6) versus 82.1(75.1-89.0), p = 0.939; BV, 75.8(68.2-83.5) versus 74.4(66.4-82.3), p = 0.793; BY, 94.2(90.0-98.4) versus 92.3(87.5-97.1), p = 0.568). Additionally, FD-subunit NAIIV4 has comparable safety and better seroprotection versus that of the half-dose in 6-35 months toddlers groups ([Total adverse reaction rates (95%CI)], 37.5(18.5-56.5) versus 47.5(26.1-68.9), p = 0.366) ([Seroprotection rates (95%CI)], H1N1, 85(56.4-100.0) versus 75.7(47.6-100.0), p = 0.117; H3N2, 50(28.1-71.9) versus 29.7(12.2-47.3), p = 0.070; BV, 75(48.2-100.0) versus 29.7(12.2-47.3), p < 0.001; BY, 75(48.2-100.0) versus 56.8(32.5-81.0), p = 0.091). As a result, the FD-subunit NAIIV4 we developed is safe and effective to provide broader and adequate protection against the circulating influenza viruses during 2018-2019, which could be an essential component of the global preventive strategy for influenza pandemic.
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http://dx.doi.org/10.1016/j.vaccine.2021.05.070DOI Listing
June 2021

Microscopic deformation mechanism and main influencing factors of carbon nanotube coated graphene foams under uniaxial compression.

Nanotechnology 2021 Jun 3;32(34). Epub 2021 Jun 3.

Institute of Advanced Structure Technology, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.

Many experiments have shown that carbon nanotube-coated (CNT-coated) graphene foam (CCGF) has specific mechanical properties, which further expand the application of graphene foam materials in many advanced fields. To reveal the microscopic deformation mechanism of CCGF under uniaxial compression and the main factors affecting their mechanical properties, numerical experiments based on the coarse-grained molecular dynamics method are systematically carried out in this paper. It is found that the relative stiffness of CNTs and graphene flakes seriously affects the microscopic deformation mechanism and strain distribution in CCGFs. The bar reinforcing mechanism will dominate the microstructural deformation in CCGFs composed of relatively soft graphene flakes, while the microstructural deformation in those composed of stiff graphene flakes will be dominated by the mechanical locking mechanism. The effects of CNT fraction, distribution of CNTs on graphene flakes, the thickness of graphene flakes, and the adhesion strength between CNTs and graphene flakes on the initial and intermediate moduli of foam materials are further studied in detail. The results of this paper should be helpful for a deep understanding of the mechanical properties of CCGF materials and the optimization design of microstructures in advanced graphene-based composites.
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http://dx.doi.org/10.1088/1361-6528/ac020cDOI Listing
June 2021

A novel approach for the preparation of core-shell MOF/polymer composites as mixed-mode stationary phase.

Talanta 2021 Sep 8;232:122459. Epub 2021 May 8.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu, 730000, China. Electronic address:

The nickel organic framework capped with polyvinylpyrrolidone was prepared and synergistically immobilized onto porous silica surface as the mixed-mode stationary phase for high-performance liquid chromatography. Here, polyvinylpyrrolidone firstly was chosen as functional molecules to change morphology and size of the metal organic framework. The silica microspheres were then modified by them via a simple bonding method rather than in-situ growth method with the aid of electrostatic interaction commonly used before. The stationary phase showed flexible selectivity for separation of both hydrophilic and hydrophobic compounds, especially for hydrophilic compounds such as carbohydrates, alkaloids and sulfonamides etc. The chromatographic behaviors were evaluated by investigating various factors, and a typical mixed-mode retention feature of the column was observed. The composites could be prepared repetitively, and relative standard deviations of retention time of objective compounds among different batches were less than 1.75%. It also showed excellent chromatographic reproducibility, stability and potentiality for application in real samples. In short, the composites can be used for a feasible option for analysis of multiple compounds as the mixed-mode stationary phase and it provides a general approach for preparing MOFs-based composites by changing morphology and size of MOFs.
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http://dx.doi.org/10.1016/j.talanta.2021.122459DOI Listing
September 2021

Electronegativity Assisted Synthesis of Magnetically Recyclable Ni/NiO/g-CN for Significant Boosting H Evolution.

Materials (Basel) 2021 May 28;14(11). Epub 2021 May 28.

Department of Chemistry and Pharmacy, Qingdao Agricultural University, Qingdao 266000, China.

A magnetically recyclable Ni/NiO/g-CN photocatalyst with significantly enhanced H2 evolution efficiency was successfully synthesized by a simple ethanol-solvothermal treatment. The presence of electronegative g-CN is found to be the key factor for Ni formation in ternary Ni/NiO/g-CN, which provides anchoring sites for Ni absorption and assembling sites for Ni nanoparticle formation. The metallic Ni, on one side, could act as an electron acceptor enhancing carrier separation and transfer efficiency, and on the other side, it could act as active sites for H evolution. The NiO forms a p-n heterojunction with g-CN, which also promotes carrier separation and transfer efficiency. The strong magnetic property of Ni/NiO/g-CN allows a good recyclability of catalyst from aqueous solution. The optimal Ni/NiO/g-CN showed a full-spectrum efficiency of 2310 μmol·h·g for hydrogen evolution, which is 210 times higher than that of pure g-CN. This ethanol solvothermal strategy provides a facile and low-cost synthesis of metal/metal oxide/g-CN for large-scale application.
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http://dx.doi.org/10.3390/ma14112894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199054PMC
May 2021

Improving the Performance of ZnS Photocatalyst in Degrading Organic Pollutants by Constructing Composites with AgO.

Nanomaterials (Basel) 2021 May 30;11(6). Epub 2021 May 30.

School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory of Molecular Engineering, Qilu University of Technology, Jinan 250353, China.

ZnS is a promising photocatalyst in water purification, whereas its low photon efficiency and poor visible-light response restrict its application. Constructing composites may help solve these problems. In this work, AgO was introduced to ZnS for the first time based on their energy band characteristics to form a novel ZnS/AgO composite photocatalyst. In the model reaction of degrading methylene blue, the as-designed catalyst exhibited high catalytic activity among a series of ZnS-based composite photocatalysts under similar conditions. The catalytic rate constant was up to 0.138 min, which is 27.4- and 15.6-times higher than those of ZnS and AgO. This composite degraded 92.4% methylene blue in 50 min, while the ratios were 31.9% and 68.8% for ZnS and AgO. Catalytic mechanism study based on photoluminescence and radical-scavenging experiments revealed that the enhanced photocatalytic activity was attributed to the composite structure of ZnS/AgO. The structure not only facilitated the separation and transmission of photogenerated carriers but also extended the light response range of the catalyst. The as-designed ZnS/AgO composite is promising in degrading organic pollutants in water.
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http://dx.doi.org/10.3390/nano11061451DOI Listing
May 2021

Chemical Analyses and Antimicrobial Activity of Nine Kinds of Unifloral Chinese Honeys Compared to Manuka Honey (12+ and 20+).

Molecules 2021 May 8;26(9). Epub 2021 May 8.

College of Animal Science, Zhejiang University, No. 866, Yuhangtang Road, Xihu District, Hangzhou 310058, China.

Honey has good antimicrobial properties and can be used for medical treatment. The antimicrobial properties of unifloral honey varieties are different. In this study, we evaluated the antimicrobial and antioxidant activities of nine kinds of Chinese monofloral honeys. In addition, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) technology was used to detect their volatile components. The relevant results are as follows: 1. The agar diffusion test showed that the diameter of inhibition zone against of Fennel honey (21.50 ± 0.41 mm), Agastache honey (20.74 ± 0.37 mm), and Pomegranate honey (18.16 ± 0.11 mm) was larger than that of Manuka 12+ honey (14.27 ± 0.10 mm) and Manuka 20+ honey (16.52 ± 0.12 mm). The antimicrobial activity of Chinese honey depends on hydrogen peroxide. 2. The total antioxidant capacity of Fennel honey, Agastache honey, and Pomegranate honey was higher than that of other Chinese honeys. There was a significant positive correlation between the total antioxidant capacity and the total phenol content of Chinese honey (r = 0.958). The correlation coefficient between the chroma value of Chinese honey and the total antioxidant and the diameter of inhibition zone was 0.940 and 0.746, respectively. The analyzed dark honeys had better antimicrobial and antioxidant activities. 3. There were significant differences in volatile components among Fennel honey, Agastache honey, Pomegranate honey, and Manuka honey. Hexanal-D and Heptanol were the characteristic components of Fennel honey and Pomegranate honey, respectively. Ethyl 2-methylbutyrate and 3-methylpentanoic acids were the unique compounds of Agastache honey. The flavor fingerprints of the honey samples from different plants can be successfully built using HS-GC-IMS and principal component analysis (PCA) based on their volatile compounds. Fennel honey, Agastache honey, and Pomegranate honey are Chinese honey varieties with excellent antimicrobial properties, and have the potential to be developed into medical grade honey.
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http://dx.doi.org/10.3390/molecules26092778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125924PMC
May 2021

Adjuvant treatment and molecular mechanism of probiotic compounds in patients with gastric cancer after gastrectomy.

Food Funct 2021 May 30. Epub 2021 May 30.

Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Gastrectomy is the main treatment for gastric cancer (GC) at present. Surgery improves the survival rate of patients, but the complications seriously affect the recovery and lack effective treatment measures. In the present study, probiotic compounds (4 strains; Lactobacillus plantarum MH-301 (CGMCC NO. 18618), L. rhamnosus LGG-18 (CGMCC NO. 14007), L. acidophilus and Bifidobacterium animalis subsp.lactis LPL-RH (CGMCC NO. 4599)), through clinical and animal model verification, were studied to try to find the auxiliary treatment measures after gastrectomy, and explore its potential mechanism. Clinical research results showed that probiotic compounds treatment could significantly lower postoperative inflammation, enhance immunity, resume gut microbiota composition and promote postoperative recovery. The results in rat models indicated that gastrostomy led to the aggravation of inflammation, the impairment of immunity and intestinal barrier, and the disorder of gut microbiota in vivo. Furthermore, probiotic compounds' administration could downregulate the inflammatory and permeability signaling pathways in the intestinal tissue, reduce the levels of proinflammatory factors, maintain the intestinal mucosal barrier and immune function, and recover the disorder of gut microbiota after gastrectomy in rats. Therefore, we conclude that probiotic compounds can restore gut microbiota homeostasis, reduce inflammation, maintain intestinal mucosal barrier and immunity, finally promote recovery after gastrectomy, and is expected to improve the prognosis of patients.
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http://dx.doi.org/10.1039/d1fo01375kDOI Listing
May 2021

Reproducible coactivation patterns of functional brain networks reveal the aberrant dynamic state transition in schizophrenia.

Neuroimage 2021 May 25;237:118193. Epub 2021 May 25.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, Center for Information in Medicine, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, China; Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ 07102, United States.

It is well documented that massive dynamic information is contained in the resting-state fMRI. Recent studies have identified recurring states dominated by similar coactivation patterns (CAPs) and revealed their temporal dynamics. However, the reproducibility and generalizability of the CAP analysis are unclear. To address this question, the effects of methodological pipelines on CAP are comprehensively evaluated in this study, including the preprocessing, network construction, cluster number and three independent cohorts. The CAP state dynamics are characterized by the fraction of time, persistence, counts, and transition probability. Results demonstrate six reliable CAP states and their dynamic characteristics are also reproducible. The state transition probability is found to be positively associated with the spatial similarity. Furthermore, the aberrant CAP states in schizophrenia have been investigated by using the reproducible method on three cohorts. Schizophrenia patients spend less time in CAP states that involve the fronto-parietal network, but more time in CAP states that involve the default mode and salience network. The aberrant dynamic characteristics of CAP states are correlated with the symptom severity. These results reveal the reproducibility and generalizability of the CAP analysis, which can provide novel insights into the neuropathological mechanism associated with aberrant brain network dynamics of schizophrenia.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118193DOI Listing
May 2021

[Spatio-Temporal Evolution Characteristics and Source Apportionment of O and NO in Shijiazhuang].

Huan Jing Ke Xue 2021 Jun;42(6):2679-2690

Shijiazhuang Meteorological Bureau, Shijiazhuang 050081, China.

Ground-level O, NO, and meteorological (temperature, humidity, wind speed, precipitation, and sunshine duration) data from 18 counties in Shijiazhuang City from 2014 to 2017, and volatile organic compounds (VOCs) data for Summer 2017, were analyzed to explore the spatial patterns, evolution, influencing factors, and source apportionment of O and NO in Shijiazhuang City. Network analysis and inverse distance weighted (IDW) spatial autocorrelation and backward trajectories analyses were performed. The results indicate that O concentrations increased between 2014 and 2017, and monthly variations showed a unimodal trend. The typical period of peak O pollution (O ≥ 160 μg·m) was from May to September, characterized by high temperatures, low humidity, weak winds, and strong solar radiation. The O concentrations were negatively correlated with the NO. Furthermore, O concentrations increased year-on-year since 2015 in main urban area, and the dominant pollutant type had changed from NO (2014 to 2016) to VOCs (2016 and 2017). However, the O concentration of county-areas limited by the VOCs. The main factors affecting O concentrations were industry, agriculture, economy, and population, and centers of O pollution associated with secondary industry appeared in the main urban areas of Shijiazhuang and Luancheng. Moreover, VOCs trajectories during the summer monitoring period were clustered in three source directions:(A) East-northeast, 26.67%; (B) Northwest-west, 43.33%; and (C) Southeast-south, 30%). Trajectories (A) and (C) were the dominant directions of VOC transmission (east-southeast).
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http://dx.doi.org/10.13227/j.hjkx.202011090DOI Listing
June 2021

Characterization and phylogenetic analysis of the chloroplast genome of var. .

Mitochondrial DNA B Resour 2021 Apr 27;6(4):1490-1491. Epub 2021 Apr 27.

College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

var. is a well-regarded ornamental plant in the genus (family Iridaceae). In this report, we present the complete chloroplast (cp) genome sequence of var. for the first time. The complete cp genome of var. was assembled using high-throughput sequencing, and phylogenetic analysis was undertaken based on a dataset of coding regions. The cp genome of var. measures 152,409 bp in length, with regions having two inverted copies (IR 26,026 bp), and separated by the large single copy (LSC 82,256 bp) and small single copy (SSC 18,101 bp) regions. The cp genome encodes 133 unique genes, including 87 different protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Based on a dataset of 69 chloroplast coding regions, the maximum-likelihood (ML) phylogenetic tree analysis indicated that var. clusters closely with . Thus, the complete chloroplast genome presented in this report may provide valuable genetic information not only for the future exploitation and utilization of this plant resource but also for further research investigating its relationship with other species.
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http://dx.doi.org/10.1080/23802359.2020.1847611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081313PMC
April 2021

Immunogenicity and safety of hepatitis B vaccination in patients with type 2 diabetes in China: An open-label randomized controlled trial.

Vaccine 2021 Jun 13;39(25):3365-3371. Epub 2021 May 13.

Department of Laboratorial Science, School of Public Health, Peking University, Beijing, China. Electronic address:

Objective: Hepatitis B virus (HBV) infection remains a global public health challenge. Patients with diabetes are at greater risk of HBV infection than healthy people. The immunogenicity and safety of two major hepatitis B vaccines were evaluated in Chinese patients with diabetes.

Methods: In this phase IV, open-label, randomized, controlled study, participants with diabetes were randomly recruited from 6 townshiphealthcenters in Gansu Province and received either a 3-dose Saccharomyces cerevisiae recombinant hepatitis B vaccine (Group D20SC 0-1-6, n = 113) or a 3-dose Chinese hamster ovary cell (CHO) recombinant hepatitis B vaccine (Group D20CHO 0-1-6, n = 119). Healthy control groups were randomly recruited from the same 6 health centers and received 3 doses of the saccharomyces cerevisiae recombinant hepatitis B vaccine (Group ND20SC 0-1-6, n = 77). Immunogenicity, including seroconversion rate and geometric mean concentration (GMC) at 1 month after three doses of vaccination, and safety were assessed. The seroconversion rate was defined as the concentration of HBsAb ≥ 10 mIU/mL.

Results: The seroconversion rates of Group D20SC 0-1-6, Group D20CHO 0-1-6 and Group ND20SC 0-1-6 were 89.6%, 91.4% and 97.1%, respectively, in the per-protocol analysis, and these differences were not statistically significant. The antibody concentration in Group D20SC 0-1-6 (GMC = 601 mIU/mL) was lower than that of the healthy control group (GMC = 1465 mIU/mL), but no significant difference was found in the GMC between the Group D20CHO 0-1-6 (GMC = 778 mIU/mL) and Group D20SC 0-1-6. Adverse events (AEs) were comparable between groups, and no serious AEs were found in these three groups.

Conclusions: The Saccharomyces cerevisiae recombinant and CHO recombinant hepatitis B vaccines in China can induce good immunogenicity in a diabetic population, although the antibody concentration may be lower, indicating the feasibility of vaccinating a large number of diabetic patients in China with these vaccines.
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http://dx.doi.org/10.1016/j.vaccine.2021.04.058DOI Listing
June 2021

SS-31 Protects Liver from Ischemia-Reperfusion Injury via Modulating Macrophage Polarization.

Oxid Med Cell Longev 2021 13;2021:6662156. Epub 2021 Apr 13.

Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Ischemia-reperfusion injury (IRI) is a common complication in liver surgeries. It is a focus to discover effective treatments to reduce ischemia-reperfusion injury. Previous studies show that oxidative stress and inflammation response contribute to the liver damage during IRI. SS-31 is an innovated mitochondrial-targeted antioxidant peptide shown to scavenge reactive oxygen species and decrease oxidative stress, but the protective effects of SS-31 against hepatic IRI are not well understood. The aim of our study is to investigate whether SS-31 could protect the liver from damages induced by IRI and understand the protective mechanism. The results showed that SS-31 treatment can significantly attenuate liver injury during IRI, proved by HE staining, serum ALT/AST, and TUNEL staining which can assess the degree of liver damage. Meanwhile, we find that oxidative stress and inflammation were significantly suppressed after SS-31 administration. Furthermore, the mechanism revealed that SS-31 can directly decrease ROS production and regulate STAT1/STAT3 signaling in macrophages, thus inhibiting macrophage M1 polarization. The proinflammation cytokines are then significantly reduced, which suppress inflammation response in the liver. Taken together, our study discovered that SS-31 can regulate macrophage polarization through ROS scavenging and STAT1/STAT3 signaling to ameliorate liver injury; the protective effects against hepatic IRI suggest that SS-31 may be an appropriate treatment for liver IRI in the clinic.
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http://dx.doi.org/10.1155/2021/6662156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057883PMC
May 2021

Sub-5 nm single crystalline organic p-n heterojunctions.

Nat Commun 2021 May 13;12(1):2774. Epub 2021 May 13.

Beijing National Laboratory for Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, Beijing, China.

The cornerstones of emerging high-performance organic photovoltaic devices are bulk heterojunctions, which usually contain both structure disorders and bicontinuous interpenetrating grain boundaries with interfacial defects. This feature complicates fundamental understanding of their working mechanism. Highly-ordered crystalline organic p-n heterojunctions with well-defined interface and tailored layer thickness, are highly desirable to understand the nature of organic heterojunctions. However, direct growth of such a crystalline organic p-n heterojunction remains a huge challenge. In this work, we report a design rationale to fabricate monolayer molecular crystals based p-n heterojunctions. In an organic field-effect transistor configuration, we achieved a well-balanced ambipolar charge transport, comparable to single component monolayer molecular crystals devices, demonstrating the high-quality interface in the heterojunctions. In an organic solar cell device based on the p-n junction, we show the device exhibits gate-tunable open-circuit voltage up to 1.04 V, a record-high value in organic single crystalline photovoltaics.
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http://dx.doi.org/10.1038/s41467-021-23066-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119440PMC
May 2021

Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein.

Vet Microbiol 2021 Jul 4;258:109099. Epub 2021 May 4.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Poxviruses have evolved multiple strategies to modulate host-derived factors to create an optimal environment for viral efficient replication. Our previous study indicated that cyclophilin B (CypB) is a critical factor for ORFV replication in MDBK cells. However, the precise molecular mechanism by which CypB facilitates ORFV replication remains less understood. In the present study, the function of CypB in ORFV replication is further evaluated. The overexpression of CypB was observed to facilitate ORFV replication in OFTu cells and HeLa cells, however, RNA interference (RNAi)-mediated reduction of endogenous CypB decreased the levels of ORFV replication. Coimmunoprecipitation experiments revealed that the CypB interacted with ORFV ORF058 protein, a late protein involved in virus entry. The interaction of host factor CypB and ORF058 protein was further confirmed by confocal microscopy analysis and GST-pull down. In addition, the 52-55 aa was identified as the critical binding sites for CypB on ORF058 protein by GST-pull down with OFTu cells overexpressing CypB and purified GST-tagged truncated ORF058. In conclusion, we demonstrate that CypB is a critical host factor for ORFV replication in vitro by interacting with ORF058 protein, providing new insights into ORFV pathogenesis.
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http://dx.doi.org/10.1016/j.vetmic.2021.109099DOI Listing
July 2021