Publications by authors named "Shuai Huang"

322 Publications

Drug repositioning based on network-specific core genes identifies potential drugs for the treatment of autism spectrum disorder in children.

Comput Struct Biotechnol J 2021 1;19:3908-3921. Epub 2021 Jul 1.

Clinical Data Center, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong, China.

Identification of exact causative genes is important for drug repositioning based on drug-gene-disease relationships. However, the complex polygenic etiology of the autism spectrum disorder (ASD) is a challenge in the identification of etiological genes. The network-based core gene identification method can effectively use the interactions between genes and accurately identify the pathogenic genes of ASD. We developed a novel network-based drug repositioning framework that contains three steps: network-specific core gene (NCG) identification, potential therapeutic drug repositioning, and candidate drug validation. First, through the analysis of transcriptome data for 178 brain tissues, gene network analysis identified 365 NCGs in 18 coexpression modules that were significantly correlated with ASD. Second, we evaluated two proposed drug repositioning methods. In one novel approach (dtGSEA), we used the NCGs to probe drug-gene interaction data and identified 35 candidate drugs. In another approach, we compared NCG expression patterns with drug-induced transcriptome data from the Connectivity Map database and found 46 candidate drugs. Third, we validated the candidate drugs using an in-house mental diseases and compounds knowledge graph (MCKG) that contained 7509 compounds, 505 mental diseases, and 123,890 edges. We found a total of 42 candidate drugs that were associated with mental illness, among which 10 drugs (baclofen, sulpiride, estradiol, entinostat, everolimus, fluvoxamine, curcumin, calcitriol, metronidazole, and zinc) were postulated to be associated with ASD. This study proposes a powerful network-based drug repositioning framework and also provides candidate drugs as well as potential drug targets for the subsequent development of ASD therapeutic drugs.
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http://dx.doi.org/10.1016/j.csbj.2021.06.046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280514PMC
July 2021

Phosphorylation of Cofilin-1 Enhances Paclitaxel Resistance of Epithelial Ovarian Cancer Cells by Inhibiting Apoptosis.

Biomed Environ Sci 2021 Jun;34(6):465-477

Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China.

Objective: To investigate the molecular mechanism of high phosphorylation levels of cofilin-1 (p-CFL-1) associated with paclitaxel resistance in epithelial ovarian cancer (EOC) cells.

Methods: Cells displaying varying levels of p-CFL-1 and CFL-1 were created by plasmid transfection and shRNA interference. Cell inhibition rate indicating paclitaxel efficacy was assessed by Cell Counting Kit-8 (CCK-8) assay. Apoptosis was assessed by flow cytometry and protein levels were detected by western blotting. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression levels of phosphokinases and phosphatases of CFL-1. Survival analysis evaluated the correlation between the prognosis of EOC patients and the levels of p-CFL-1 and slingshot-1 (SSH-1).

Results: High levels of p-CFL-1 were observed in EOC cells that survived treatment with high doses of paclitaxel. SKOV3 cell mutants with upregulated p-CFL-1 showed impaired paclitaxel efficacy, as well as decreased apoptosis rates and pro-survival patterns of apoptosis-specific protein expression. Cytoplasmic accumulation of p-CFL-1 inhibited paclitaxel-induced mitochondrial apoptosis. SSH-1 silencing mediated CFL-1 phosphorylation in paclitaxel-resistant SKOV3 cells. Clinically, the high level of p-CFL-1 and the low level of SSH-1 in EOC tissues were closely related to chemotherapy resistance and poor prognosis in EOC patients.

Conclusion: The SSH-1/p-CFL-1 signaling pathway mediates paclitaxel resistance by apoptosis inhibition in EOC and is expected to be a potential prognostic predictor.
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http://dx.doi.org/10.3967/bes2021.063DOI Listing
June 2021

Integrating Constituents Absorbed into Blood, Network Pharmacology, and Quantitative Analysis to Reveal the Active Components in var. that Regulate Lipid Metabolism Disorder.

Front Pharmacol 2021 29;12:630198. Epub 2021 Jun 29.

School of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.

var. (S. K. Lee) L. T. Lu (RS)-a sweet plant also known as Tiancha distributed in the south of China where it is used as a beverage-recently gained extensive attention as adjuvant therapy of diabetes and hypertension. Although pharmacological studies indicate that RS has beneficial effects in regulating lipid metabolism disorder characteristics, the active chemicals responsible for this effect remains unclear. The present study aims to predict the effective substances of RS on regulating lipid metabolism disorder through the analysis of the chemical profile of RS, the absorbed prototype components in rat plasma, and network pharmacology. Also, a UPLC method able to quantify the screened potential effective chemicals of RS products was established. First, a total of 69 components-including diterpene, triterpenoids, flavonoids, polyphenols, and lignans-were systematically characterized in RS. Of those, 50 compounds were detected in the plasma of rats administered with RS extract. Through network pharmacology, 9 potential effective components, 71 target genes, and 20 pathways were predicted to be involved in RS-mediated regulation of lipid metabolism disorder. The quantitative analysis suggested that the contents of potential effective components varied among samples from different marketplaces. In conclusion, the presented results provide a chemical basis for further research of var. .
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http://dx.doi.org/10.3389/fphar.2021.630198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282055PMC
June 2021

Covalent Adaptable Liquid Crystal Networks Enabled by Reversible Ring-Opening Cascades of Cyclic Disulfides.

J Am Chem Soc 2021 Jul 18. Epub 2021 Jul 18.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing 211189, China.

The development of covalent adaptable liquid crystal networks (LCNs) enabled by introducing dynamic covalent bonds has endowed liquid crystal actuators with self-healing properties and reversible shape programmability, broadening their applications in diverse soft robotic devices. However, the finite molecular design strategy limits the recyclability and the architectural diversity of these materials. Here, a strategy is first reported for fabricating photoresponsive polydisulfide-based covalent adaptable LCNs by ring-opening polymerization of cyclic dithiolane groups. Based on the disulfide metathesis, the resulting materials are self-healable, reshapable, and reprogrammable. Importantly, the equilibrium between the polymer backbones and the dithiolane-functionalized monomers enables catalytic depolymerization to recycle monomers, which could significantly weaken the disadvantage of subtractive manufacturing of photomechanical devices. This work rooted in chemistry would provide an economical and environmentally friendly strategy for the fabrication of functional soft robotics with excellent programmability and renewability and beyond.
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http://dx.doi.org/10.1021/jacs.1c03661DOI Listing
July 2021

Diterpenoid alkaloids from the whole herb of Delphinium grandiflorum L.

Phytochemistry 2021 Jul 14;190:112866. Epub 2021 Jul 14.

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, PR China; Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, 610031, Sichuan, PR China. Electronic address:

Seven previously undescribed diterpenoid alkaloids, eight reaction products and thirteen known compounds were isolated from the whole plant of Delphinium grandiflorum L. (Ranunculaceae). Grandiflonines A and B have an unprecedented C-diterpenoid alkaloid skeleton, which features inversion of the configuration of C-18. Their structures were determined by comprehensive analyses of spectroscopic data, X-ray diffraction and Mosher's method. The probable biosynthetic pathway of grandiflonine A was discussed. Additionally, the analgesic activity and anti-inflammatory activity by inhibition of NO production were evaluated. Among them, deoxylappaconitine (ED = 0.35 mg/kg, TI = 46.22) showed significant analgesic activity that was superior to the reference drug lappaconitine (ED = 3.5 mg/kg, TI = 3.34).
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http://dx.doi.org/10.1016/j.phytochem.2021.112866DOI Listing
July 2021

A new diterpenoid alkaloid from C. Marquand & Airy Shaw.

Nat Prod Res 2021 Jul 9:1-6. Epub 2021 Jul 9.

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, P.R. China.

A new C-diterpenoid alkaloid named gyalanutine A () and fourteen known compounds - were isolated from the plant of C. Marquand & Airy Shaw. Compound displayed an unusual lycoctonine-type C-diterpenoid alkaloid skeleton with the cleavage of -C and C-C bonds, and the construction of the -C bond. Structures were identified by multiple spectroscopic analyses including 1 D, 2 D NMR, IR and HR-ESI-MS. Compounds were tested for acetylcholinesterase inhibitory and anti-inflammatory activity.
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http://dx.doi.org/10.1080/14786419.2021.1948043DOI Listing
July 2021

Effects of interventional therapy on AQP4 gene expression and neuron apoptosis in rabbits with ischemic brain injury caused by carotid artery stenosis.

Int J Clin Exp Pathol 2021 15;14(6):786-793. Epub 2021 Jun 15.

Department of Neurosurgery, The Sixth People's Hospital of Nantong Nantong 226011, Jiangsu Province, China.

: To explore the effects of interventional therapy (IT) on Aquaporin-4 (AQP4) gene expression and neuron apoptosis in rabbits with ischemic brain injury caused by carotid artery stenosis, and to further optimize the therapeutic regime of clinical ischemic brain injury (IBI). : 30 New Zealand rabbits were randomly divided into three groups: Sham group (n=10), IBI group (n=10) and IBI+IT group (n=10). A rabbit model of carotid artery stenosis was established to induce IBI. 12 hours later, interventional therapy was achieved through percutaneous transluminal coronary angioplasty (PTCA) by pumping up the balloon to widen the catheter. The expression of AQP4 mRNA and protein were determined by reverse transcription polymerase chain reaction (RT-PCR) and western blot, respectively. The distributions of rabbit hippocampus and cerebral cortex were detected by Nissl staining. The neuronal function of rabbits in the three groups after surgery was assessed with mNSS score. TUNEL (Terminal Transferase-mediated dUTP Nick End-labelling) staining was used to observe neuron apoptosis in rabbit brain tissue. The expressions of Bax and Bcl-2 protein were detected by western blot. Markers of oxidative stress in rabbit brain tissues were detected by Reactive Oxygen Species ELISA Kits. Morphology of the organelles in rabbit brain tissues was observed with transmission electron microscopy (TEM). : The protein and mRNA expressions of AQP4 in rabbit brain tissues were significantly decreased in the IBI group. Nissl staining results showed that IT improved IBI in rabbit hippocampus. In addition, IT significantly ameliorated the neuronal function of rabbits, and reduced the apoptosis and oxidative stress level of neurons in brain tissues. Furthermore, we found that after IT, organelle damage was significantly reduced in rabbit neurons. : After receiving IT, AQP4 gene level and neuron apoptosis were significantly reduced in rabbit brain tissues with ischemic brain injury from carotid artery stenosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255198PMC
June 2021

Identification of the molecular mechanisms underlying brisket disease in Holstein heifers via microbiota and metabolome analyses.

AMB Express 2021 Jun 12;11(1):86. Epub 2021 Jun 12.

College of Animal Science, Xinjiang Agricultural University, Urumqi, 830052, China.

Brisket disease (BD) is common among Holstein heifers in high-altitude environments, and this disease may result in serious economic loss. At present, no effective treatment is available for brisket disease. In this study, liver and cecum samples were collected from five heifers with BD and five healthy heifers (HH) for analyses of the metabolome and microbiota. The mean pulmonary arterial pressure and systolic blood pressure were significantly higher in BD heifers, whereas the average breathing rate, blood oxygen saturation, and glucose level were significantly lower in BD group than in the HH group. Further, 16S rDNA data showed that the abundance of Firmicutes was significantly lower and that of Bacteroidetes was significantly higher in BD group than in the HH group. At the genus level, the BD group heifers harbored fewer Ruminococcaceae and Lachnospiraceae than the HH group. Several metabolites, including beta-D-fructose, D-ribose, 1,4-beta-D-glucan, sucrose, and glucose-6-phosphate were present at low levels in BD heifers. Moreover, the mean pulmonary arterial pressure was negatively correlated with beta-D-fructose (r =  - 0.74; P = 0.013), D-ribose (r =  - 0.72; P = 0.018), and acetyl-tyrosine-ethyl-ester (r =  - 0.71; P = 0.022). We also found that mean pulmonary arterial pressure was negatively correlated with most of the genera, including those in the families of Lachnospiraceae and Ruminococcaceae. In summary, the decreased levels of metabolites and microbial genera might affect BD by limiting the energy supply. This study may help us better understand the role of the microbiota in BD and provide new insights into the management of feeding to decrease the rate of BD in Holstein dairy cows in the Qinghai-Tibetan plateau.
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http://dx.doi.org/10.1186/s13568-021-01246-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241945PMC
June 2021

Construction of a novel asymmetric imidazole-cored AIE probe for ratiometric imaging of endogenous leucine aminopeptidase.

Chem Commun (Camb) 2021 Jul;57(54):6608-6611

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, P. R. China. and Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha 410078, China.

We report a rational strategy to deliberately construct the first asymmetric tetraarylimidazole-based AIE probe, integrating AIE behavior in synergy with ESIPT character to image endogenous LAP for the first time. It offered good sensitivity and selectivity, and concomitantly, was applied successfully for real-time tracking of LAP in the cisplatin-induced liver injury zebrafish model.
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http://dx.doi.org/10.1039/d1cc01940fDOI Listing
July 2021

Circ_HECW2 regulates LPS-induced apoptosis of chondrocytes via miR-93 methylation.

Immun Inflamm Dis 2021 Jun 2. Epub 2021 Jun 2.

Department of Sports Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Introduction: Circ_HECW2 plays a key role in lipopolysaccharide (LPS)-induced signal transduction, which is critical in osteoarthritis (OA). Thus, we analyzed the role of Circ_HECW2 in osteoarthritis.

Methods: The expression of Circ_HECW2 and miR-93 was examined using reverse-transcription polymerase chain reaction. Cell apoptosis was evaluated using Annexin V-FITC Apoptosis Detection Kit.

Results: Circ_HECW2 and miR-93 were inversely correlated, with Circ_HECW2 upregulated and miR-93 downregulated in OA and LPS-induced chondrocytes. Circ_HECW2 overexpression inhibited miR-93 expression and increased methylation of miR-93 coding gene. Cell apoptosis analysis showed that Circ_HECW2 overexpression increased LPS-induced chondrocyte apoptosis, while MiR-93 overexpression reversed the effects of Circ_HECW2 on chondrocyte apoptosis.

Conclusion: In summary, our data revealed that the Circ_HECW2 is highly expressed in OA and might inhibit miR-93 expression through methylation to affect LPS-induced chondrocyte apoptosis.
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http://dx.doi.org/10.1002/iid3.453DOI Listing
June 2021

A phase-separated nuclear GBPL circuit controls immunity in plants.

Nature 2021 Jun 26;594(7863):424-429. Epub 2021 May 26.

Howard Hughes Medical Institute, New Haven, CT, USA.

Liquid-liquid phase separation (LLPS) has emerged as a central paradigm for understanding how membraneless organelles compartmentalize diverse cellular activities in eukaryotes. Here we identify a superfamily of plant guanylate-binding protein (GBP)-like GTPases (GBPLs) that assemble LLPS-driven condensates within the nucleus to protect against infection and autoimmunity. In Arabidopsis thaliana, two members of this family-GBPL1 and GBPL3-undergo phase-transition behaviour to control transcriptional responses as part of an allosteric switch that is triggered by exposure to biotic stress. GBPL1, a pseudo-GTPase, sequesters catalytically active GBPL3 under basal conditions but is displaced by GBPL3 LLPS when it enters the nucleus following immune cues to drive the formation of unique membraneless organelles termed GBPL defence-activated condensates (GDACs) that we visualized by in situ cryo-electron tomography. Within these mesoscale GDAC structures, native GBPL3 directly bound defence-gene promoters and recruited specific transcriptional coactivators of the Mediator complex and RNA polymerase II machinery to massively reprogram host gene expression for disease resistance. Together, our study identifies a GBPL circuit that reinforces the biological importance of phase-separated condensates, in this case, as indispensable players in plant defence.
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http://dx.doi.org/10.1038/s41586-021-03572-6DOI Listing
June 2021

Healable and Rearrangeable Networks of Liquid Crystal Elastomers Enabled by Diselenide Bonds.

Angew Chem Int Ed Engl 2021 Jul 17;60(30):16394-16398. Epub 2021 Jun 17.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Southeast University, Nanjing, 211189, China.

Based on liquid crystal elastomer (LCE) materials, hierarchically structured soft actuators can meet some requirements for "human-friendly" working mode and execute complex tasks with intelligent adaptation to environmental changes. However, few researchers have paid much attention to the preparation methods of multicomponent/hierarchical LCE actuators. In this communication, we demonstrate the successful integration of an exchangeable diselenide chain extender for the preparation of dynamic LCEs, which could be reprogrammed on heating or under visible light illumination. Moreover, the rearrangeable polydiselenide networks could be applied to develop the self-welding technology toward fabricating hierarchically structured LCE actuators with sophisticated deformability without using any auxiliary reagent (adhesive, tape, catalysts or initiator) during the assembling process.
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http://dx.doi.org/10.1002/anie.202105278DOI Listing
July 2021

Pristimerin synergistically sensitizes conditionally reprogrammed patient derived-primary hepatocellular carcinoma cells to sorafenib through endoplasmic reticulum stress and ROS generation by modulating Akt/FoxO1/p27 signaling pathway.

Phytomedicine 2021 Jun 4;86:153563. Epub 2021 Apr 4.

Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, China. Electronic address:

Background: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-associated mortality worldwide. Sorafenib (SORA), as a first-line therapeutic drug, has been used to treat HCC, but resistance poses a major limitation on the efficacy of SORA chemotherapy. Pristimerin (PRIS), a natural bioactive component isolated from various plant species in the Celastraceae and Hippocrateaceae families, has been reported to exhibit outstanding antitumor effects in several types of cells in vitro.

Purpose: The aim of this study was to investigate whether PRIS can exert synergistic anti-tumor effects with the combination of SORA, and if so, through what mechanism.

Methods: Conditionally reprogrammed patient derived-primary hepatocellular carcinoma cells (CRHCs) were isolated from human liver cancer tissues and treated with SORA and PRIS. Cell proliferation, apoptosis, migration and tube formation ability were detected by DNA content quantification, flow cytometry, transwell assay and Matrigel-based angiogenesis assay. Gene and protein expression were assessed by qRT-PCR and Western blot respectively.

Results: Initially, we observed that the combination of the two drugs had a much stronger inhibitory effect on CRHCs growth than either drug alone. Moreover, the combination of 2 µM SORA and 1 µM PRIS exhibited a significant anti‑migrative and anti-invaded effect on CRHCs, and remarkably inhibited capillary structure formation of Human Umbilical Vein Endothelial Cells (HUVECs). Furthermore, the combined treatment with SORA and PRIS synergistically induced intrinsic apoptosis in CRHCs, involving a caspase-4-dependent mechanism paralleled by an increased Bax/Bcl-xL ratio. These activities were mediated through ROS generation and the induction of endoplasmic reticulum (ER) stress and mitochondrial dysfunction. GRP78 silencing or ER stress inhibitor 4-phenylbutyric acid administration was revealed to abolish the anticancer effects of PRIS, indicating the critical role of GRP78 in mediating the bioactivity of PRIS. The present study also provides mechanistic evidence that PRIS modulated the Akt/FoxO1/p27 signaling pathway, which is required for mitochondrial-mediated intrinsic apoptosis, activation of ER stress, and stimulation of caspase-4 induced by PRIS, and, consequently resulting in suppressed cell viability, migration and angiogenesis co-treated with SORA in CRHCs.

Conclusion: Our results suggest the use of PRIS as sensitizers of chemotherapy paving the way for innovative and promising targeted chemotherapy-based therapeutic strategies in human HCC.
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http://dx.doi.org/10.1016/j.phymed.2021.153563DOI Listing
June 2021

Identification of the Potential Role of the Rumen Microbiome in Milk Protein and Fat Synthesis in Dairy Cows Using Metagenomic Sequencing.

Animals (Basel) 2021 Apr 26;11(5). Epub 2021 Apr 26.

National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

The rumen contains abundant microorganisms that aid in the digestion of lignocellulosic feed and are associated with host phenotype traits. Cows with extremely high milk protein and fat percentages (HPF; = 3) and low milk protein and fat percentages (LPF; = 3) were selected from 4000 lactating Holstein cows under the same nutritional and management conditions. We found that the total concentration of volatile fatty acids, acetate, butyrate, and propionate in the rumen fluid was significantly higher in the HPF group than in the LPF group. Moreover, we identified 38 most abundant species displaying differential richness between the two groups, in which accounted for 68.8% of the species, with the highest abundance in the HPF group. Functional annotation based on the Kyoto Encyclopedia of Gene and Genome (KEGG), evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG), and Carbohydrate-Active enzymes (CAZy) databases showed that the significantly more abundant species in the HPF group are enriched in carbohydrate, amino acid, pyruvate, insulin, and lipid metabolism and transportation. Furthermore, Spearman's rank correlation analysis revealed that specific microbial taxa (mainly the species and ) are positively correlated with total volatile fatty acids (VFA). Collectively, we found that the HPF group was enriched with several species related to the total VFA, acetate, and amino acid synthesis. Thereby, these fulfilled the host's needs for energy, fat, and rumen microbial protein, which can be used for increased biosynthesis of milk fat and milk protein. Our findings provide novel information for elucidation of the regulatory mechanism of the rumen in the formation of milk composition.
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http://dx.doi.org/10.3390/ani11051247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146572PMC
April 2021

Delayed treatment with Endostatin displays a protective role against pulmonary hypertension by targeting VEGF pathway.

Minerva Med 2021 Apr 29. Epub 2021 Apr 29.

Department of Bone and Joint Surgery, Shandong Provincial Qianfoshan Hospital, the First Hospital Affiliated with Shandong First Medical University, Jinan, Shandong, China -

Background: Endostatin (ES) is an endogenous angiogenesis inhibitor. It is confirmed that ES has antitumor effects and plays a crucial part in regulating vascular smooth cells' proliferation. However, ES's effect on pulmonary hypertension (PH) is unclear. We aimed to determine the effect of ES on PH's pathogenesis.

Methods: PH was induced by pneumonectomy plus monocrotaline (MCT) injection, as indicated with significantly increased pulmonary arterial pressure and vascular wall thickness.

Results: Immunohistochemical analysis showed that under physiological conditions, ES localized in endothelial cells (ECs) and spread to the muscular vascular layers in PH rats. ES was transfected into the lungs of rats intratracheally 2 weeks after MCT injection. Consequently, ES not only reduced elevated VEGF's expression but also reversed pulmonary artery remodeling. Eventually, ES improved elevated right ventricular (RV) mean pressure and RV hypertrophy.

Conclusions: The administration of ES may be a new treatment for PH and PA remodeling, associating with the down-regulation of VEGF production.
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http://dx.doi.org/10.23736/S0026-4806.21.07145-7DOI Listing
April 2021

Magnetically Responsive Superhydrophobic Surface with Switchable Adhesivity Based on Electrostatic Air Spray Deposition.

ACS Appl Mater Interfaces 2021 May 27;13(17):20885-20896. Epub 2021 Apr 27.

National Engineering Research Center for High Efficiency Grinding, Hunan University, Changsha 410082, P. R. China.

A new method was reported for preparing a magnetically responsive superhydrophobic surface by electrostatic air spray deposition (EASD) and magnetic induction. The mixture was fully atomized under the combined action of the electrostatic field and the high-speed airflow field, and a dense array of micropillars was formed. The atomization mechanism of EASD was explored. The distribution and physical parameters of the micropillars were evaluated and counted. Switchable adhesion characteristics of the surface and the reversibility in 10 cycles were examined. The influences of different electrostatic voltages, component concentration, spray distance, air pressure, and magnetic field intensity on the surface morphology and hydrophobicity were analyzed. The prepared surface can be reversibly transformed between the high-adhesion state (with a contact angle of 108°) and the low-adhesion state (with a contact angle of 154°) by on/off switching of an external magnetic field. After a 2.2 kPa pressure load was applied, the surface contact angle was 144° with an applied magnetic field of 0.4 T. After heated at 90 °C for more than 90 min, the surface can almost obtain superhydrophobicity (with a contact angle of 148°) in the absence of a magnetic field. By utilizing the switchable surface adhesion characteristics, various kinds of droplet transmissions were realized. When the cured surface was spray-coated with carbon nanoparticles (CNPs), active droplet manipulation can be achieved by simply moving the magnet. The advantages of this method include a simple preparation process without chemical surface modification.
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http://dx.doi.org/10.1021/acsami.1c04003DOI Listing
May 2021

Supramolecular Chirality Transfer toward Chiral Aggregation: Asymmetric Hierarchical Self-Assembly.

Adv Sci (Weinh) 2021 04 1;8(8):2002132. Epub 2021 Mar 1.

Advanced Materials and Liquid Crystal Institute and Chemical Physics Interdisciplinary Program Kent State University Kent OH 44242 USA.

Self-assembly, as a typical bottom-up strategy for the fabrication of functional materials, has been applied to fabricate chiral materials with subtle chiral nanostructures. The chiral nanostructures exhibit great potential in asymmetric catalysis, chiral sensing, chiral electronics, photonics, and even the realization of several biological functions. According to existing studies, the supramolecular chirality transfer process combined with hierarchical self-assembly plays a vital role in the fabrication of multiscale chiral structures. This progress report focuses on the hierarchical self-assembly of chiral or achiral molecules that aggregate with asymmetric spatial structures such as twisted bands, helices, and superhelices in different environments. Herein, recent studies on the chirality transfer induced self-assembly based on a variety of supramolecular interactions are summarized. In addition, the influence of different environments and the states of systems including solutions, condensed states, gel systems, interfaces on the asymmetric hierarchical self-assembly, and the expression of chirality are explored. Moreover, both the driving forces that facilitate chiral bias and the supramolecular interactions that play an important role in the expression, transfer, and amplification of the chiral sense are correspondingly discussed.
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http://dx.doi.org/10.1002/advs.202002132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061372PMC
April 2021

Light-driven continuous rotating Möbius strip actuators.

Nat Commun 2021 Apr 20;12(1):2334. Epub 2021 Apr 20.

Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, China.

Twisted toroidal ribbons such as the one-sided Möbius strip have inspired scientists, engineers and artists for many centuries. A physical Möbius strip exhibits interesting mechanical properties deriving from a tendency to redistribute the torsional strain away from the twist region. This leads to the interesting possibility of building topological actuators with continuous deformations. Here we report on a series of corresponding bi-layered stripe actuators using a photothermally responsive liquid crystal elastomer as the fundamental polymeric material. Employing a special procedure, even Möbius strips with an odd number of twists can be fabricated exhibiting a seamless homeotropic and homogeneous morphology. Imposing a suitable contraction gradient under near-infrared light irradiation, these ribbons can realize continuous anticlockwise/clockwise in-situ rotation. Our work could pave the way for developing actuators and shape morphing materials that need not rely on switching between distinct states.
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http://dx.doi.org/10.1038/s41467-021-22644-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058083PMC
April 2021

Computational exploration of natural peptides targeting ACE2.

J Biomol Struct Dyn 2021 Apr 7:1-12. Epub 2021 Apr 7.

CAS Key Laboratory of Separation Sciences of Analytical Chemistry, Dalian Institute of Chemical Physics, Dalian, China.

Interaction between the SARS-COV-2 (2019 novel coronavirus) spike protein and ACE2 receptors expressed on cellular surfaces initialises viral attachment and consequent infection. Blocking this interaction shows promise for blocking or ameliorating the virus' pathological effects on the body. By contrast to work focusing on the coronavirus, which has significant potential diversity through possible accumulation of mutations during transmission, targeting the conserved ACE2 protein expressed on human cells offers an attractive alternative route to developing pharmacological prophylactics against viral invasion. In this study, we screened a virtual database of natural peptides , with ACE2 as the target, and performed structural analyses of the interface region in the SARS-COV-2 RBD/ACE2 complex. These analyses have identified 15 potentially effective compounds. Analyses of ACE2/polypeptide interactions suggest that these peptides can block viral invasion of cells by stably binding in the ACE2 active site pocket. Molecular simulation results for Complestatin and Valinomycin indicate that they may share this mechanism. The discovery of this probable binding mechanism provides a frame of reference for further optimization, and design of high affinity ACE2 inhibitors that could serve as leads for production of drugs with preventive and therapeutic effects against SARS-COV-2.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2021.1905555DOI Listing
April 2021

Seismic performance analysis of a wind turbine tower subjected to earthquake and ice actions.

PLoS One 2021 31;16(3):e0247557. Epub 2021 Mar 31.

Institute of Crustal Dynamics, China Earthquake Administration, Beijing, PR China.

Sea ice is one of the main loads acting on a wind turbine tower in areas prone to icing, and this threatens safe working life of the wind turbine tower. In our study, a simplified calculated model of ice, wind turbine tower, and water dynamic interaction under earthquake action was proposed, which could avoid to solve a large number of nonlinear equations. Then, the seismic behaviour of the wind turbine tower with and without the influence of sea ice was investigated, and we found that the influence of the greater mass of the sea ice on the seismic response of a wind turbine tower should be considered when the wind turbine tower is designed in an area with thick ice. With the influence of the most unfavourable ice mass, the deformation and energy dissipation capacity of the wind turbine tower are decreased, and the wall thickness or stiffening rib thickness should be increased to improve the seismic performance and ductility of the wind turbine tower; the shear force and bending moment increased significantly on the wind turbine tower, and the shear force changes at the bottom of the wind turbine tower and position of action of the sea ice: attention should be paid to the wind turbine tower design at these positions. Finally, we conducted the shaking table test, and verified the rationality of our proposed simplified model.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247557PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011770PMC
March 2021

Seismic behaviour analysis of a wind turbine tower affected by sea ice based on a simplified model.

Sci Rep 2021 Mar 24;11(1):6714. Epub 2021 Mar 24.

China Coal Research Institute, Beijing, 100013, China.

Ice-structure interaction threatens the safety of the offshore structure; however, dynamic seismic action even renders this process more sophisticated. This research constructed a simplified calculation model for the wind turbine tower, ice, and water under seismic loading, which could avoid solving the complex non-linear equations. Then, the seismic behaviour of the structure, i.e. wind turbine tower, in the presence and absence of influences of the sea ice was investigated, and we found the remarkable effect of sea ice upon the wind turbine tower when its mass is within a range; the wind turbine tower is found to have reduced capacity in energy dissipation, and thickness of tower walls or stiffening ribs is supposed to be enlarged for making the structure more ductile. Affected by the sea ice, the shear force and bending moment of the tower showed significant increases, and more attention needs to be paid to the tower bottom and action position of the sea ice. According to the dynamic similarity principle, finally paraffin was used to simulate sea ice, and shaking-table tests were performed for simulating dynamic ice-structure-water interactions. Results of shaking-table tests verified the rationality of our proposed simplified model.
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http://dx.doi.org/10.1038/s41598-021-86142-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991629PMC
March 2021

The Rumen Bacterial Community in Dairy Cows Is Correlated to Production Traits During Freshening Period.

Front Microbiol 2021 4;12:630605. Epub 2021 Mar 4.

State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.

The rumen microbiome plays a vital role in providing nutrition to the host animal, thereby influencing ruminant production. Despite its importance, it is not fully understood how variation in the ruminal bacteria community composition influences dry matter intake (DMI), milk yield and ruminal fermentative parameters in dairy cows, especially during freshening period. Here, we hypothesized that during early lactation, high DMI cows having a different ruminal microbiota than low DMI cows, and that this difference persists over time. To test this, we enrolled 65 fresh and determinzed their DMI using an auto-feed intake recording system. Fourteen days after calving, the 10 animals with the lowest (LFI) and the 10 animals with the highest (HFI)-average DMI were selected for further analysis. Rumen fluid was collected from these two cohorts at 1 (Fresh1d) and 14 days (Fresh14d) after calving and their ruminal microbiota were assessed using 16S rRNA sequencing. Volatile fatty acid (VFA) concentrations were also quantified. Comparison of the ruminal microbiotas between Fresh1d and Fresh14d showed that Fresh14d cows had a significantly higher relative abundance of VFA-producing microbes ( < 0.05), such as and . This was commensurate with the concentrations of acetate, propionate, butyrate, valerate and total VFAs, were also significantly ( < 0.05) increased in Fresh14d cows. We also found that the differences in the ruminal microbiota between LFI and HFI cows was limited, but DMI significantly altered ( < 0.05) the relative proportion of bacteria in the families , and . Furthermore, specific operational taxonomic units belonging to the was significantly ( < 0.05) correlated with DMI and milk yield. Taking together, our findings provide a framework for future studies of freshening period cow that seek to better understand the role of the ruminal microbiota during this critical period in the lactation cycle.
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http://dx.doi.org/10.3389/fmicb.2021.630605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969525PMC
March 2021

Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2.

Cell Commun Signal 2021 Mar 18;19(1):35. Epub 2021 Mar 18.

School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325000, People's Republic of China.

Background: The mechanism underlying endothelial dysfunction leading to cardiovascular disease in type 2 diabetes mellitus (T2DM) remains unclear. Here, we show that inhibition of histone deacetylase 3 (HDAC3) reduced inflammation and oxidative stress by regulating nuclear factor-E2-related factor 2 (Nrf2), which mediates the expression of anti-inflammatory- and pro-survival-related genes in the vascular endothelium, thereby improving endothelial function.

Methods: Nrf2 knockout (Nrf2 KO) C57BL/6 background mice, diabetic db/db mice, and control db/m mice were used to investigate the relationship between HDAC3 and Nrf2 in the endothelium in vivo. Human umbilical vein endothelial cells (HUVECs) cultured under high glucose-palmitic acid (HG-PA) conditions were used to explore the role of Kelch-like ECH-associated protein 1 (Keap1) -Nrf2-NAPDH oxidase 4 (Nox4) redox signaling in the vascular endothelium in vitro. Activity assays, immunofluorescence, western blotting, qRT-PCR, and immunoprecipitation assays were used to examine the effect of HDAC3 inhibition on inflammation, reactive oxygen species (ROS) production, and endothelial impairment, as well as the activity of Nrf2-related molecules.

Results: HDAC3 activity, but not its expression, was increased in db/db mice. This resulted in de-endothelialization and increased oxidative stress and pro-inflammatory marker expression in cells treated with the HDAC3 inhibitor RGFP966, which activated Nrf2 signaling. HDAC3 silencing decreased ROS production, inflammation, and damage-associated tube formation in HG-PA-treated HUVECs. The underlying mechanism involved the Keap1-Nrf2-Nox4 signaling pathway.

Conclusion: The results of this study suggest the potential of HDAC3 as a therapeutic target for the treatment of endothelial dysfunction in T2DM. Video Abstract.
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http://dx.doi.org/10.1186/s12964-020-00681-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977318PMC
March 2021

Role of exosomes in pancreatic cancer.

Oncol Lett 2021 Apr 17;21(4):298. Epub 2021 Feb 17.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

Pancreatic cancer (PC) is a malignant tumour of the human digestive system that has a poor prognosis. Exosomes contain proteins and nucleic acids, and constitute a class of extracellular vesicles defined as membrane-bound nanovesicles of endocytic origin, with a diameter of 40-150 nm. Exosomes are potential diagnostic markers of PC; however, their roles in cancer initiation and progression remain unclear. Previous studies have focused on the molecular mechanisms and functions of exosomes that allow them to accelerate PC cell proliferation, migration and invasion. The present review discusses the interactions between exosomes and the pathophysiology of PC. The potential clinical applications of exosomes are also discussed.
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http://dx.doi.org/10.3892/ol.2021.12559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905690PMC
April 2021

Convenient Preparation of -Acylbenzoxazines from Phenols, Nitriles, and DMSO Initiated by a Catalytic Amount of (COCl).

J Org Chem 2021 04 17;86(7):4932-4943. Epub 2021 Mar 17.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China.

A convenient preparation method of -acylbenzoxazines has been developed, in which phenols react with nitriles and dimethyl sulfoxide (DMSO) in the presence of a catalytic amount of (COCl) in CHCN or chloroform to afford the corresponding -acylbenzoxazines in moderate-to-good yields. DMSO acts as a source of HCHO, which is generated in situ from the decomposition of a methoxydimethylsulfonium salt. A regeneration cycle of the methoxydimethylsulfonium salt is proposed, which is initiated by a catalytic amount of (COCl).
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http://dx.doi.org/10.1021/acs.joc.0c02768DOI Listing
April 2021

High efficiency tunable unidirectional single-longitudinal-mode Er:YAG ring laser based on an acousto-optic modulator.

Opt Express 2021 Mar;29(5):6445-6452

A wavelength tunable single-longitudinal-mode (SLM) Er:YAG ring laser around 1.6 µm is demonstrated. By using an acousto-optic modulator (AOM) to force unidirectional operation, up to 10.4 W and 8.7 W SLM laser output power are obtained at 1645.22 nm and 1617.33 nm, with corresponding slope efficiencies of 45% and 40%, respectively. Besides, stable dual-wavelength operation at both 1645 nm and 1617 nm is also achieved with the maximum power of 9.1 W. By rotating the birefringent filter (BRF) in the ring cavity, the wavelength could be tuned from 1616.77 nm to 1617.51 nm and 1644.51 nm to 1646.12 nm. The line width is measured to be 125 kHz at 1617 nm and 131 kHz at 1645 nm via the time-delayed self-heterodyne method. As far as we know, 8.7 W is the highest continuous-wave SLM output power at 1617 nm.
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http://dx.doi.org/10.1364/OE.416284DOI Listing
March 2021

Biosyntheses of geranic acid and citronellic acid from monoterpene alcohols by Saccharomyces cerevisiae.

Biosci Biotechnol Biochem 2021 May;85(6):1530-1535

Department of Applied Biological Chemistry, School of Agriculture, Utsunomiya University, Tochigi, Japan.

Geraniol is one of the important aromatic ingredients in alcoholic beverages. Bioconversions of geraniol to other terpenoids and genes involved in the oxidation of geraniol were investigated. Geranic acid and citronellic acid were detected in yeast culture, where geraniol or nerol was added. Addition of citral, a mixture of geranial and neral, resulted in the production of geranic acid and citronellic acid, whereas the addition of citral or citronellal resulted in the production of citronellic acid, suggesting that citronellic acid might be produced through the conversion of citral to citronellal followed by the oxidation of citronellal. Consumption of geraniol and production of geranic acid, citronellic acid, and citronellol were affected in adh1Δ, adh3Δ, adh4Δ, and sfa1Δ yeast strains, which possess single deletion of a gene encoding alcohol dehydrogenase. This is the first report of the bioconversion of monoterpene alcohols, geraniol and nerol, to geranic acid and citronellic acid in yeast culture.
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http://dx.doi.org/10.1093/bbb/zbab039DOI Listing
May 2021

bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway.

Redox Biol 2021 05 20;41:101904. Epub 2021 Feb 20.

School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325000, PR China. Electronic address:

Protein S-nitrosylation is a reversible protein modification implicated in both physiological and pathophysiological regulation of protein function. However, the relationship between dysregulated S-nitrosylation homeostasis and diabetic vascular complications remains incompletely understood. Here, we demonstrate that basic fibroblast growth factor (bFGF) is a key regulatory link between S-nitrosylation homeostasis and inflammation, and alleviated endothelial dysfunction and angiogenic defects in diabetes. Subjecting human umbilical vein endothelial cells (HUVECs) to hyperglycemia and hyperlipidemia significantly decreased endogenous S-nitrosylated proteins, including S-nitrosylation of inhibitor kappa B kinase β (IKKβ) and transcription factor p65 (p65), which was alleviated by bFGF co-treatment. Pretreatment with carboxy-PTIO (c-PTIO), a nitric oxide scavenger, abolished bFGF-mediated S-nitrosylation increase and endothelial protection. Meanwhile, nitrosylation-resistant IKKβ and p65 mutants exacerbated endothelial dysfunction in db/db mice, and in cultured HUVECs subjected to hyperglycemia and hyperlipidemia. Mechanistically, bFGF-mediated increase of S-nitrosylated IKKβ and p65 was attributed to synergistic effects of increased endothelial nitric oxide synthase (eNOS) and thioredoxin (Trx) activity. Taken together, the endothelial protective effect of bFGF under hyperglycemia and hyperlipidemia can be partially attributed to its role in suppressing inflammation via the S-nitrosylation pathway.
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http://dx.doi.org/10.1016/j.redox.2021.101904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972985PMC
May 2021

Construction and theoretical insights into the ESIPT fluorescent probe for imaging formaldehyde in vitro and in vivo.

Chem Commun (Camb) 2021 Apr 10;57(28):3496-3499. Epub 2021 Mar 10.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, P. R. China.

We report the first ESIPT-based probe ABTB, for the highly sensitive and selective imaging of formaldehyde (FA). The various theoretical calculations have been systematically performed, and clearly unravel the lighting mechanism of the fluorescent probe for FA. Additionally, the probe was successfully applied in monitoring endogenous FA in the brain of AD mice.
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http://dx.doi.org/10.1039/d1cc00429hDOI Listing
April 2021

Correction to: Dynamic changes of the fecal bacterial community in dairy cows during early lactation.

AMB Express 2021 Mar 6;11(1):40. Epub 2021 Mar 6.

The State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

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http://dx.doi.org/10.1186/s13568-021-01185-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936995PMC
March 2021
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