Publications by authors named "Shuai Guo"

232 Publications

Effect of Steam on the Tar Reforming during Circulating Fluidized Bed Char Gasification.

ACS Omega 2021 May 18;6(17):11192-11198. Epub 2021 Mar 18.

Institute of Engineering Thermophysics, Chinese Academy of Sciences, Beijing 100190, China.

In this work, the real tar was introduced into the circulating fluidized bed gasifier by pre-mixing tar and char. The effect of steam on the tar reforming characteristics at both 850 and 900 °C was investigated by combining the analysis of the rate of tar conversion, the change of tar content, and char physical structure. The test results indicated that steam could effectively promote the tar conversion. Therefore, the content of tar in the final gas could be reached as low as 32 mg/Nm. It was found that the effect of steam on the different components of tar was in difference. Among the various components, polycyclic aromatic substances were more inclined to decompose. The results of BET confirmed that the distribution and structure of pore were obviously developed at the presence of steam, and the abundant pore structure further improved the catalytic performance of the char on the tar conversion in turn.
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http://dx.doi.org/10.1021/acsomega.0c05861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153945PMC
May 2021

The Effects of Exercise Interventions on Balance Capacity in Patients with Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis.

Inquiry 2021 Jan-Dec;58:469580211018284

College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.

The effect of exercise intervention on balance capacity among type 2 diabetes mellitus (T2DM) patients has not been evaluated. The objective of this systematic review and meta-analysis is to investigate the effect of exercise intervention on balance capacity among T2DM patients compared to the control group (usual care, waitlist, no-treatment, education). We conducted a comprehensive literature search through PubMed, EMBASE, Physiotherapy Evidence Database (PEDro), Cochrane library, Web of Science (WOS) from inception to August 2020. The literature language was limited to English. Randomized controlled trials (RCTs) or quasi-experimental (Q-E) trials that examined the effect of exercise intervention on balance capacity among T2DM patients were included. We used the standard methods of meta-analysis to evaluate the outcomes of exercise intervention for balance capacity of T2DM patients. A total of 14 trials (11 RCTs and 3 Q-E trials) involving 883 participants were eligible. The meta-analysis of some studies demonstrated that exercise intervention could significantly improve Berg Balance Scale (BBS) (MD = 2.56; 95%CI [0.35, 4.77];  = .02), SLST (Single Leg Stance Test) under the eyes-open (EO) condition (MD = 3.63; 95%CI [1.79, 5.47];  = .0001) and eyes-close (EC) condition (MD = 0.41; 95%CI [0.10, 0.72];  = .01) compared to control group. There was no significant difference in Time Up and Go Test (TUGT) (MD = -0.75; 95%CI [-1.69, 0.19];  = .12) and fall efficacy (SMD = -0.44; 95%CI [-0.86, -0.01];  = .05). Narrative review of some studies indicated that exercise intervention could improve postural stability measured by Sensory Organization Test (SOT) and Center of Pressure (COP) variables, etc. This systematic review and meta-analysis summarized that exercise intervention could improve balance capacity in T2DM patients. However, further studies with high quality are required to evaluate its effect.
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http://dx.doi.org/10.1177/00469580211018284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155768PMC
May 2021

A path planning method of 6-DOF robot for mirror therapy based on A* algorithm.

Technol Health Care 2021 May 14. Epub 2021 May 14.

Background: With the population aging, post-stroke patients suffering from hemiplegia are also rapidly increasing. It is essential to provide valid rehabilitation methods for hemiplegia patients. Mirror therapy is an effective rehabilitation method and is widely applied in many rehabilitation robots.

Objective: The aim of this paper is to present a path planning method to guarantee the robot's motion performance during mirror therapy.

Methods: The kinematic framework of the proposed rehabilitation system is detailed, then the reference motion path of the manipulator is calculated according to kinematic transformation. The concept of manipulability is introduced to describe the motion performance of the manipulator. Based on the above work, a path planning method based on A* algorithm is proposed to quantitatively analyze and optimize the motion performance of the manipulator.

Results: Preliminary experiments with the proposed rehabilitation system are conducted to verify the proposed path planning method. The characteristics of the proposed method are analyzed through two typical situations. The results showed that the proposed method can build a new path for manipulator, which can ensure the robot's motion performance and is highly consistent with the reference path.

Conclusion: The results showed that the manipulator could achieve the task with acceptable error, which indicates the potential of the proposed path planning method for mirror therapy.
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http://dx.doi.org/10.3233/THC-202551DOI Listing
May 2021

Metabolic footprint analysis of volatile metabolites by gas chromatography-ion mobility spectrometry to discriminate between different fermentation temperatures during Streptococcus thermophilus milk fermentation.

J Dairy Sci 2021 May 20. Epub 2021 May 20.

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China. Electronic address:

Streptococcus thermophilus is widely used in the dairy industry to produce fermented milk. Gas chromatography-ion mobility spectrometry-based metabolomics was used to discriminate different fermentation temperatures (37°C and 42°C) at 3 time points (F0: pH = 6.50 ± 0.02; F1: pH = 5.20 ± 0.02; F2: pH = 4.60 ± 0.02) during S. thermophilus milk fermentation, and differences of fermentation physical properties and growth curves were also evaluated. Fermentation was completed (pH 4.60) after 6 h at 42°C and after 8 h at 37°C; there were no significant differences in viable cell counts and titratable acidity; water-holding capacity and viscosity were higher at 37°C than at 42°C. Different fermentation temperatures affected volatile metabolic profiles. After the fermentation was completed, the volatile metabolites that could be used to distinguish the fermentation temperature were hexanal, butyraldehyde, ethyl acetate, ethanol, 3-methylbutanal, 3-methylbutanoic acid, and 2-methylpropionic acid. Specifically, at 37°C of milk fermentation, branched-chain AA had higher levels, and leucine, isoleucine, and valine were involved in growth and metabolism, which promoted accumulation of some short-chain fatty acids such as 3-methylbutanoic acid and 2-methylpanprooic acid. At 42°C, at 3 different time points during fermentation, ethanol from glycolysis all presented higher levels, including acetone and 3-methylbutanal, producing a more pleasant flavor in the fermented milk. This work provides detailed insight into S. thermophilus fermented milk metabolites that differed between incubation temperatures; these data can be used for understanding and eventually predicting metabolic changes during milk fermentation.
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http://dx.doi.org/10.3168/jds.2020-19555DOI Listing
May 2021

Long noncoding RNA POU6F2-AS1 regulates lung cancer aggressiveness through sponging miR-34c-5p to modulate KCNJ4 expression.

Genet Mol Biol 2021 14;44(2):e20200050. Epub 2021 May 14.

Shandong Chest Hospital, Department of Respiratory Medicine, Jinan, Shandong, China.

It has been extensively reported that long noncoding RNAs (lncRNAs) were closely associated with multiple malignancies. The aim of our study was to investigate the effects and mechanism of lncRNA POU6F2-AS1 in lung adenocarcinoma (LADC).The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets provided us the information of LADC clinical samples. High-regulation of POU6F2-AS1 was presented in LADC tissues compared with adjacent normal tissues, which was correlated with poor outcome of LADC patients. Functional experiments in Calu-3 and NCI-H460 cells showed that POU6F2-AS1 significantly promoted LADC cell proliferation, colony formation, invasion and migration. Moreover, through online prediction, luciferase reporter assay and Pearson's correlation analysis, we found that POU6F2-AS1 may act as a competing endogenous RNA (ceRNA) of miR-34c-5p and facilitated the expression of potassium voltage-gated channel subfamily J member 4 (KCNJ4). The promoting effect of cell aggressiveness induced by POU6F2-AS1 was enhanced by KCNJ4, whilst was abrogated due to the overexpression of miR-34c-5p. Collectively, POU6F2-AS1 might function as a ceRNA through sponging miR-34c-5p to high-regulate KCNJ4 in LADC, which indicates that POU6F2-AS1 might be a promising therapeutic target with significant prognostic value for LADC treatment.
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http://dx.doi.org/10.1590/1678-4685-GMB-2020-0050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127722PMC
May 2021

MicroRNA-211 regulates the expression of TAB1 and inhibits the NF-κB signaling pathway in lipopolysaccharide-induced endometritis.

Int Immunopharmacol 2021 May 10;96:107668. Epub 2021 May 10.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address:

Endometritis is a common postpartum inflammatory disease that endangers the reproductive health of humans and animals. Emerging evidence shows that microRNA is a new type of therapeutic molecule that plays a vital role in many diseases; however, its mechanism of action in lipopolysaccharide (LPS)-induced endometritis is still unclear. This study aims to investigate the regulatory role of miR-211 in the innate immune response involved in endometritis, and to evaluate its potential therapeutic value. Here, we found that the expression of miR-211 in bovine endometrial epithelial cells (bEECs) stimulated by lipopolysaccharide (LPS) was significantly reduced. Importantly, overexpression of miR-211 can significantly reduce the production of pro-inflammatory cytokines (IL-1β , IL-6 and TNF-α). In addition, we proved that TAB1 is the target gene of miR-211. MiR-211 inhibits TAB1 protein expression by binding to the 3'-UTR of TAB1 mRNA. Subsequently, we verified that the overexpression of miR-211 inhibited the activation of NF-κB p65 by targeting the TAB1-mediated pathway. Therefore, miR-211 has anti-inflammatory effects and mediates the negative regulation of the NF-κB signaling pathway in LPS-induced endometritis by targeting TAB1.
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http://dx.doi.org/10.1016/j.intimp.2021.107668DOI Listing
May 2021

Cadmium disturbs epigenetic modification and induces DNA damage in mouse preimplantation embryos.

Ecotoxicol Environ Saf 2021 Aug 11;219:112306. Epub 2021 May 11.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, PR China. Electronic address:

Cadmium is an environmental pollutant that has extensive deleterious effects on the reproductive system. However, the mechanisms underlying the effects of cadmium on preimplantation embryos are unclear. Here, we used a mouse model to investigate the effects of maternal cadmium (32 mg/l) exposure in drinking water for 2 days on early embryonic development, and studied the mechanisms associated with epigenetic modifications and DNA damage induced by oxidative stress. We observed that maternal cadmium exposure impaired preimplantation embryo development by inducing embryo death, fragmentation, or developmental blockade. After cadmium exposure, the most survived embryos were at the 8-cell stage, which were used for all measurements. Histone acetylation, not methylation, was disturbed by increasing histone deacetylase 1 (HDAC1) levels after cadmium exposure. Cadmium also disrupted DNA methylation of H19; however genomic DNA methylation can be normally reprogrammed in embryos. Furthermore, cadmium increased reactive oxygen species (ROS) levels and DNA damage, and partly inhibited gene expression related to DNA repair. The distribution and activity of mitochondria was increased; therefore, embryos maintain intracellular homeostasis for survival. Collectively, our findings revealed that maternal cadmium exposure impairs preimplantation embryo development by disturbing the epigenetic modification and inducing DNA damage.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112306DOI Listing
August 2021

Inhibition of Small-Conductance, Ca-Activated K Current by Ondansetron.

Front Pharmacol 2021 22;12:651267. Epub 2021 Apr 22.

Division of Cardiology, Department of Medicine, The Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, IN, United States.

Small-conductance Ca-activated K channels (SK channels) have been proposed as antiarrhythmic targets for the treatment of atrial fibrillation. We previously demonstrated that the 5-HT receptor antagonist ondansetron inhibits heterologously expressed, human SK2 (hSK2) currents as well as native cardiac SK currents in a physiological extra-/intracellular [K] gradient at therapeutic (i.e., sub-micromolar) concentrations. A recent study, using symmetrical [K] conditions, challenged this result. The goal of the present study was to revisit the inhibitory effect of ondansetron on hSK2-mediated currents in symmetrical [K] conditions. The whole-cell patch clamp technique was used to investigate the effects of ondansetron and apamin on hSK2-mediated currents expressed in HEK 293 cells. Currents were measured in symmetrical [K] conditions in the presence of 100 nM [Ca]. Expression of hSK2 produced inwardly rectifying whole-cell currents in the presence of 400 nM free cytosolic Ca. Ondansetron inhibited whole-cell hSK2 currents with values of 154 and 113 nM at -80 and 40 mV, respectively. Macroscopic current inhibited by ondansetron and current inhibited by apamin exhibited inwardly rectifying current-voltage relationships with similar reversal potentials (apamin, ∼5 mV and ondansetron, ∼2 mV). Ondansetron (1 μM) in the continuing presence of apamin (100 nM) had no effect on hSK2-mediated whole-cell currents. Wild-type HEK 293 cells did not express ondansetron- or apamin-sensitive currents. Ondansetron in sub-micromolar concentrations inhibits hSK2 currents even under altered ionic conditions.
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http://dx.doi.org/10.3389/fphar.2021.651267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100601PMC
April 2021

Upregulated-gene expression of pro-inflammatory cytokines, oxidative stress and apoptotic markers through inflammatory, oxidative and apoptosis mediated signaling pathways in Bovine Pneumonia.

Microb Pathog 2021 Jun 1;155:104935. Epub 2021 May 1.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. Electronic address:

Pneumonia is the acute inflammation of lung tissue and is multi-factorial in etiology. Staphylococcus aureus (S. aureus) is a harmful pathogen present as a normal flora of skin and nares of dairy cattle. In bovine pneumonia, S. aureus triggers to activates Toll-Like Receptors (TLRs), that further elicits the activation of the inflammation via NF-κB pathway, oxidative stress and apoptotic pathways. In the current study, pathogen-associated gene expression of the pro-inflammatory cytokines, oxidative stress and apoptotic markers in the lung tissue of cattle was explored in bovine pneumonia. Fifty lung samples collected from abattoir located in Wuhan city, Hubei, China. Histopathologically, thickening of alveolar wall, accumulation of inflammatory cells and neutrophils in perivascular space, hyperemia, hemorrhages and edema were observed in infected lungs as compared to non-infected lung samples. Furthermore, molecular identification and characterization were carried by amplification of S. aureus-specific nuc gene (270 base pairs) from the infected and non-infected lung samples to identify the S. aureus. Moreover, qPCR results displayed that relative mRNA levels of TLR2, TLR4, pro-inflammatory gene (IL-1β, IL-6 and TNF-α) and apoptosis-associated genes (Bax, caspase-3 and caspase-9) were up-regulated except Bcl-2, which is antiapoptotic in nature, and oxidative stress related genes (Nrf2, NQO1, HO-1 and GCLC) which was down-regulated in infected pulmonary group. The relative protein expression of NF-κB, mitochondria-mediated apoptosis gene was up-regulated while Bcl-2 and Nrf2 pathway genes were downregulated in infected cattle lungs. Our findings revealed that genes expression levels of inflammatory mediators, oxidative stress and apoptosis were associated with host immunogenic regulatory mechanisms in the lung tissue during infection. Conclusively, the present study provides insights of active immune response via TLRs-mediated inflammatory, oxidative damage, and apoptotic paradox.
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http://dx.doi.org/10.1016/j.micpath.2021.104935DOI Listing
June 2021

Inhibition of TMEM16A by Natural Product Silibinin: Potential Lead Compounds for Treatment of Lung Adenocarcinoma.

Front Pharmacol 2021 14;12:643489. Epub 2021 Apr 14.

Key Laboratory of Molecular Biophysics, Hebei Province, Institute of Biophysics, School of Sciences, Hebei University of Technology, Tianjin, China.

: Effective anticancer therapy can be achieved by identifying novel tumor-specific drug targets and screening of new drugs. Recently, TMEM16A has been identified to be overexpressed in lung adenocarcinoma, and inhibitors of TMEM16A showed obvious antitumor efficacy. : YFP fluorescence quenching and whole-cell patch clamp experiments were used to explore the inhibitory effect of silibinin on TMEM16A. Molecular docking and site-directed mutagenesis were performed to confirm the binding sites of silibinin and TMEM16A. MTT assay, wound healing assay, and annexin-V assay were used to detect the effect of silibinin on cancer cell proliferation, migration, and apoptosis. shRNA was transfected into LA795 cells to knock down the expression of endogenous TMEM16A. Tumor xenograft mice combined with Western blot experiments reveal the inhibitory effect and mechanism of silibinin . : Silibinin concentration dependently inhibited the whole-cell current of TMEM16A with an IC of 30.90 ± 2.10 μM. The putative binding sites of silibinin in TMEM16A were K384, R515, and R535. The proliferation and migration of LA795 cells were downregulated by silibinin, and the inhibition effect can be abolished by knockdown of the endogenous TMEM16A. Further, silibinin was injected to tumor xenograft mice which exhibited significant antitumor activity without weight loss. Finally, Western blotting results showed the mechanism of silibinin inhibiting lung adenocarcinoma was through apoptosis and downregulation of cyclin D1. : Silibinin is a novel TMEM16A inhibitor, and it can be used as a lead compound for the development of lung adenocarcinoma therapy drugs.
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http://dx.doi.org/10.3389/fphar.2021.643489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079988PMC
April 2021

MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis.

Anal Cell Pathol (Amst) 2021 30;2021:9652420. Epub 2021 Mar 30.

Department of Tuberculosis, Shandong Provincial Chest Hospital, Jinan, 250013 Shandong, China.

MicroRNAs (miRNAs) regulate multiple cellular behaviors, and their aberrant expression is frequently associated with disease progression. This research focused on the effects of miR-520a on the development of non-small-cell lung cancer (NSCLC) and the molecules involved. Tumor and normal tissues from 24 patients with NSCLC were collected. Differentially expressed miRNAs between tumor tissues and normal tissues were screened using microarrays, and miR-520a was screened to be significantly poorly expressed in tumor samples. Artificial upregulation of miR-520a reduced proliferation, migration and invasion, and resistance to death of NSCLC A549 and H460 cells according to the MTT, EdU labeling, transwell, and flow cytometry assays, respectively. miR-520a upregulation suppressed growth and metastasis of xenograft tumors in vivo. The integrated bioinformatic analysis and dual luciferase assays suggested that miR-520a targeted ribonucleotide reductase subunit 2 (RRM2) mRNA and inactivated the Wnt/-catenin signaling pathway in NSCLC cells. Upregulation of RRM2 enhanced the malignant behaviors of NSCLCs, but the oncogenic effects of RRM2 were blocked upon miR-520a overexpression. To conclude, this study evidenced that miR-520a inhibits NSCLC progression through suppressing RRM2 and the Wnt signaling pathway. This paper may offer novel insights into NSCLC treatment.
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http://dx.doi.org/10.1155/2021/9652420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026327PMC
March 2021

Influence of air oxidative and non-oxidative torrefaction on the chemical properties of corn stalk.

Bioresour Technol 2021 Jul 6;332:125120. Epub 2021 Apr 6.

School of Energy and Power Engineering, Northeast Electric Power University, Jilin City, Jilin 132012, PR China.

The non-structural components (extractives and ash) in biomass can affect the torrefaction characteristics. The objective of this study was to investigate the applicability of air oxidative and non-oxidative torrefaction for corn stalk, which has higher organic extractives and lower ash contents. The raw materials were torrefied with air and nitrogen in a fluidized bed reactor at 180℃~235℃ and 200℃~280℃ for 1 h, respectively. The proximate and ultimate analysis results demonstrated that the lower ash content (3.89%) was not favorable for deoxygenation. The organic extractives, extracted materials and mixture were torrefied with nitrogen at 260℃. The results indicated that the addition of organic extractives reduced the deoxidation efficiency of structural components during torrefaction. Compared to raw materials, the moderate and severe removal of hemicellulose can be achieved through torrefaction under air and nitrogen, respectively.
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http://dx.doi.org/10.1016/j.biortech.2021.125120DOI Listing
July 2021

Prognosis of C4 dislocation with spinal cord injury following psychological intervention.

J Int Med Res 2021 Apr;49(4):3000605211004520

Department of Orthopaedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objective: To investigate the effect of psychological intervention on the prognosis of patients with C4 dislocation and spinal cord injury.

Methods: We investigated target patients admitted between 2010 and 2018. Patients' mental state, quality of life and neurological function at different time points were evaluated to examine the relationship between psychological intervention and recovery and prognosis of acute and critical spinal cord injury.

Results: All patients showed improvements in clinical symptoms, neurological function and quality of life. Psychological intervention significantly improved Zung Self-Rating Anxiety Scale score, Zung Self-Rating Depression Scale score and SF-36 Mental Component Summary score within 3 months. Japanese Orthopaedic Association neurological function score and SF-36 Physical Component Summary score were significantly improved after 1-year follow-up. Psychological intervention did not improve 2-year survival.

Conclusion: Timely and professional psychological intervention can eliminate the psychological disorders of C4 dislocation patients with spinal cord injury. This has a positive effect on their quality of life and prognosis.
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http://dx.doi.org/10.1177/03000605211004520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033484PMC
April 2021

Comparison of the effects of single probiotic strains Lactobacillus casei Zhang and Bifidobacterium animalis ssp. lactis Probio-M8 and their combination on volatile and nonvolatile metabolomic profiles of yogurt.

J Dairy Sci 2021 Apr 1. Epub 2021 Apr 1.

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China; Qingdao Special Food Research Institute, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:

There has been a growing interest in cofermentation of starter cultures with probiotics in milk. In this study, we analyzed the effects of adding the single probiotics Lactobacillus casei Zhang (Zhang) or Bifidobacterium animalis ssp. lactis Probio-M8 (M8) or a combination of Zhang and M8 to starter cultures on volatile and nonvolatile metabolomic profiles after 14 d of storage at 4°C and compared using a liquid chromatography-tandem mass spectrometry (LC-MS) and GC-MS-based metabolomics approach. Principal component analysis, heatmap plots, and Spearman correlation results showed that Zhang alone had a greater effect on volatile and nonvolatile metabolomic profiles than M8 alone. The combination of Zhang and M8 had additive effects on the production of metabolites. For volatile metabolites, the levels of acetaldehyde, diacetyl, acetoin, and acetic acid were higher for the combination of Zhang and M8 compared with either single probiotic culture. Significantly increased nonvolatile components induced by adding Zhang were identified were enriched in the galactose, amino- and nucleotide sugar, fructose and mannose, purine, phenylalanine metabolism, and arginine biosynthesis pathways. The metabolism and biosynthesis of starch, sucrose, tyrosine, galactose metabolism, and aminoacyl-tRNA biosynthesis were significantly upregulated by adding the combination of Zhang and M8. This work provides a detailed insight into different effects of Zhang and M8 used alone or in combination on the volatile and nonvolatile metabolomic profiles of yogurts.
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http://dx.doi.org/10.3168/jds.2020-20099DOI Listing
April 2021

LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer.

J Cancer 2021 22;12(8):2336-2350. Epub 2021 Feb 22.

Gastric Cancer Department, Liaoning Province Cancer Hospital & Institute (Cancer Hospital of China Medical University), No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning Province, China 110042.

Disrupted gene levels are intimately correlated with the occurrence and prognosis of gastric cancer (GC). As genes do not function in isolation, we set out to investigate the possible relationship among mRNA and non-coding RNAs (ncRNAs). RNA sequencing from 406 cases of GC was acquired through the TCGA database. R packages were utilized to assess differential RNA expression. The competing endogenous RNA (ceRNA) network was predicted using miRcode, miRDB, mirTarBase, Target Scan and constructed by Cytoscape 3.6.1. GO enrichment analysis, KEGG pathway analysis, GSEA, and WGCNA were applied for pathway analysis. The expression of select candidate molecules was confirmed using western blot and RT-PCR in GC cells and tissues. CCK-8, EdU staining, and Transwell assays were conducted to assess the influence of candidate molecules on proliferation and invasion. The gain and loss-of-function were achieved by co-culture with sh-lncRNA, mimics and sh-mRNA. Luciferase reporters were created using the psiCHECK2 vector, and the relative luciferase activity was calculated. Using data from TCGA, we determined differentially expressed RNAs and created a ceRNA regulatory network. Interestingly, we identified a regulatory complex surrounding ANGPT2. We detected that ANGPT2 was highly expressed in GC, which correlated with a worse prognosis. Our findings indicated that ANGPT2 encourages growth, invasion, and epithelial-mesenchymal transition (EMT) in GC. Importantly, miR-145 inhibits ANGPT2 and abrogates its effects. Furthermore, LINC00184, a ceRNA, blocks miR-145, thereby improving ANGPT2-mediated carcinogenesis. Our findings indicate that the LINC00184/miR-145/ANGPT2 pathway has a crucial function in the development of GC and can act as a possible biomarker and targets for GC therapy.
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http://dx.doi.org/10.7150/jca.49138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974899PMC
February 2021

Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation.

Evid Based Complement Alternat Med 2021 22;2021:6657673. Epub 2021 Feb 22.

Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.

As diabetic nephropathy (DN) is one of the most common and destructive microvascular complications of diabetes mellitus, the goal of this study, therefore, was to investigate the renal protective effect and latent mechanisms of Hirudo lyophilized powder on diabetic rats. In this study, all rats were randomly assigned into the control group and diabetic group. The rats of diabetic group were injected with low-dose STZ (35 mg/kg) intraperitoneal plus high-fat diet to induce diabetes. Then, the successful diabetic model rats were weighed and randomly assigned into four groups: (1) diabetic model group (DM group); (2) Hirudo lyophilized powder 0.3 g/kg treatment group (SL group); (3) Hirudo lyophilized powder 0.6 g/kg treatment group (SM group); (4) Hirudo lyophilized powder 1.2 g/kg treatment group (SH group). Their fasting blood glucoses (FBG) were measured every 4 weeks. After treatment with Hirudo lyophilized powder at a corresponding dose once a day for 16 weeks, their metabolic and biochemical as well as oxidative stress parameters were tested, and the kidney weight (KW)/body weight (BW) was calculated. The renal tissues were used for histological, mRNA, and protein expression analysis. The results showed that Hirudo lyophilized powder could protect against the structural damages and functional changes of diabetic renal tissue by inhibiting oxidative stress, inflammation, and fibrosis. Furthermore, it was found in the further research that inhibiting the NOX4 expression and JAK2/STAT1/STAT3 pathway activation might be the underlying mechanisms. Collectively, Hirudo lyophilized powder might be a promising therapeutic agent for the treatment of DN.
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http://dx.doi.org/10.1155/2021/6657673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920712PMC
February 2021

Socio-demographic factors impact disabilities caused by perinatal asphyxia among Chinese children.

PLoS One 2021 5;16(3):e0248154. Epub 2021 Mar 5.

Institute of Population Research/WHO Collaborating Center on Reproductive Health and Population Science, Peking University, Beijing, China.

Background: Disabilities caused by perinatal asphyxia will burden child health and well-being. To date, our understanding about the situation and risk factors of perinatal asphyxia-induced disabilities among Chinese children is still limited.

Objectives: To evaluate the prevalence and socio-demographic risk factors of disabilities caused by perinatal asphyxia among Chinese children in 2006 and compare disability trajectories across different socio-demographic status.

Methods: Cross-sectional data came from the 2006 China National Survey on Disability which includes a total of 616,940 children aged 0-17 years old was employed in the investigation. Perinatal asphyxia-induced disabilities were identified by following the guidance in consensus manuals. Population-weighted numbers and prevalence rates were investigated, and multivariable logistic regression was performed to evaluate associations between disabilities and socio-demographic factors. Adjusted predictions at representative values were computed to compare the disability trajectories relative to significant socio-demographic variables.

Results: The prevalence rate of disabilities caused by perinatal asphyxia was 7.70 per 10,000 children (95% CI: 7.01-8.39). Male (OR 1.81, 95% CI: 1.47-2.23) and low family income (OR: 1.73, 95% CI: 1.21-2.49) have higher and the increase of per additional year of age (OR: 0.89, 95% CI: 0.88-0.91) has lower probability of being disabilities caused by perinatal asphyxia. Further disability trajectories showed that differences in probability between gender and family income group were more evident before age 7 and weakened with increasing age.

Conclusions: Our results showed that both demographic and socioeconomic characteristics are risk factors for disabilities caused by perinatal asphyxia. Of these, gender and family income have much higher impact than other factors on the prevalence rate of disabilities caused by perinatal asphyxia at infants and young children. Multiple society sectors should increase their effort to bring about fundamental social change to prevent disabilities caused by perinatal asphyxia, especially concerning younger children and their families.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248154PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935314PMC
March 2021

Caffeine promotes angiogenesis through modulating endothelial mitochondrial dynamics.

Acta Pharmacol Sin 2021 Mar 4. Epub 2021 Mar 4.

Guangdong Provincial People's Hospital, School of Medicine, South China University of Technology, Guangzhou, 510100, China.

Caffeine induces multiple vascular effects. In this study we investigated the angiogenic effect of physiological concentrations of caffeine with focus on endothelial cell behaviors (migration and proliferation) during angiogenesis and its mitochondrial and bioenergetic mechanisms. We showed that caffeine (10-50 μM) significantly enhanced angiogenesis in vitro, evidenced by concentration-dependent increases in tube formation, and migration of human umbilical vein endothelial cells (HUVECs) without affecting cell proliferation. Caffeine (50 μM) enhanced endothelial migration via activation of cAMP/PKA/AMPK signaling pathway, which was mimicked by cAMP analog 8-Br-cAMP, and blocked by PKA inhibitor H89, adenylate cyclase inhibitor SQ22536 or AMPK inhibitor compound C. Furthermore, caffeine (50 μM) induced significant mitochondrial shortening through the increased phosphorylation of mitochondrial fission protein dynamin-related protein 1 (Drp1) in HUVECs, which increased its activity to regulate mitochondrial fission. Pharmacological blockade of Drp1 by Mdivi-1 (10 μM) or disturbance of mitochondrial fission by Drp1 silencing markedly suppressed caffeine-induced lamellipodia formation and endothelial cell migration. Moreover, we showed that caffeine-induced mitochondrial fission led to accumulation of more mitochondria in lamellipodia regions and augmentation of mitochondrial energetics, both of which were necessary for cell migration. In a mouse model of hindlimb ischemia, administration of caffeine (0.05% in 200 mL drinking water daily, for 14 days) significantly promoted angiogenesis and perfusion as well as activation of endothelial AMPK signaling in the ischemic hindlimb. Taken together, caffeine induces mitochondrial fission through cAMP/PKA/AMPK signaling pathway. Mitochondrial fission is an integral process in caffeine-induced endothelial cell migration by altering mitochondrial distribution and energetics.
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http://dx.doi.org/10.1038/s41401-021-00623-6DOI Listing
March 2021

Illuminating kir channel function in Anderson-Tawil syndrome.

Cardiovasc Res 2021 Feb 24. Epub 2021 Feb 24.

The Krannert Institute of Cardiology and Division of Cardiology, Department of Medicine, Indiana University School of Medicine, USA.

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http://dx.doi.org/10.1093/cvr/cvab062DOI Listing
February 2021

IFN-τ Attenuates LPS-Induced Endometritis by Restraining HMGB1/NF-κB Activation in bEECs.

Inflammation 2021 Feb 18. Epub 2021 Feb 18.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Endometritis is a common inflammatory disease in uterine tissues that leads to animal infertility. Among the causes, Escherichia coli infection is one of the main reasons. Interferon-tau (IFN-τ) is the initial pregnancy signal for ruminant embryos and can induce immune tolerance in humans and other species. However, there are scarce reports on whether IFN-τ has a regulatory effect on endometrial inflammatory damage through HMGB1-NF-κB signalling. The purpose of this study was to investigate the regulatory mechanism of IFN-τ in HMGB1-NF-κB signalling in LPS-induced endometritis. ELISA and qPCR were used to detect the expression of LPS-induced pro-inflammatory cytokines in bovine endometrial epithelial cells (bEECs or BEND) under IFN-τ intervention, and the levels of HMGB1, p-IKK and p-p65 were detected by Western blotting. The nuclear translocation of NF-κB p65 was determined through immunofluorescence. In addition, bEECs were transfected with si-HMGB1 to elucidate the key role of HMGB1 and IFN-τ in the endometrial inflammatory cascade. The results indicated that IFN-τ inhibits the expression of related pro-inflammatory cytokines in an inflammatory injury model of bovine endometrial epithelial cells induced by LPS. Furthermore, experiments have proven that IFN-τ has protective effects on E. coli endotoxin-induced endometritis in mice in vivo. IFN-τ inhibited the HMGB1-NF-κB axis and significantly reduced the secretion of pro-inflammatory cytokines, the expression of HMGB1 protein and the levels of IKK and NF-κB p65 phosphorylation. In summary, our results showed that IFN-τ resists E. coli endotoxin-induced endometritis by attenuating HMGB1/NF-κB signalling.
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http://dx.doi.org/10.1007/s10753-021-01433-yDOI Listing
February 2021

Core Role of Hydrophobic Core of Polymeric Nanomicelle in Endosomal Escape of siRNA.

Nano Lett 2021 Apr 17;21(8):3680-3689. Epub 2021 Feb 17.

School of Life Science, Advanced Research Institute of Multidisciplinary Science, Institute of Engineering Medicine, Key Laboratory of Molecular Medicine and Biotherapy, Beijing Institute of Technology, Beijing 100081, China.

Efficient endosomal escape is the most essential but challenging issue for siRNA drug development. Herein, a series of quaternary ammonium-based amphiphilic triblock polymers harnessing an elaborately tailored pH-sensitive hydrophobic core were synthesized and screened. Upon incubating in an endosomal pH environment (pH 6.5-6.8), mPEG-P(DPA--DMAEMA)-PT (PDDT, the optimized polymer) nanomicelles (PDDT-Ms) and PDDT-Ms/siRNA polyplexes rapidly disassembled, leading to promoted cytosolic release of internalized siRNA and enhanced silencing activity evident from comprehensive analysis of the colocalization and gene silencing using a lysosomotropic agent (chloroquine) and an endosomal trafficking inhibitor (bafilomycin A1). In addition, PDDT-Ms/siPLK1 dramatically repressed tumor growth in both HepG2-xenograft and highly malignant patient-derived xenograft models. PDDT-Ms-armed siPD-L1 efficiently blocked the interaction of PD-L1 and PD-1 and restored immunological surveillance in CT-26-xenograft murine model. PDDT-Ms/siRNA exhibited ideal safety profiles in these assays. This study provides guidelines for rational design and optimization of block polymers for efficient endosomal escape of internalized siRNA and cancer therapy.
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http://dx.doi.org/10.1021/acs.nanolett.0c04468DOI Listing
April 2021

Effect of tai chi on glycaemic control, lipid metabolism and body composition in adults with type 2 diabetes: A meta-analysis and systematic review.

J Rehabil Med 2021 Mar 22;53(3):jrm00165. Epub 2021 Mar 22.

Rehabilitation Medicine College, Fujian University of Traditional Chinese Medicine, Fuzhou, China. E-mail:

Objective: The aim of this systematic review was to evaluate the effects of tai chi on metabolic control and body composition indicators in patients with type 2 diabetes mellitus.

Design: Systematic review and meta-analysis of existing literature.

Methods: Electronic resource databases were searched to collect eligible studies. Two reviewers selected studies and independently evaluated method-ological quality.

Results: Twenty-three studies were included in this meta-analysis. The pooled results showed that tai chi had significant effects in improving metabolic indices, such as fasting blood glucose (mean differ-ence (MD) = -1.04; 95% confidence interval (95% CI) -1.42 to 0.66; p < 0.01) and total cholesterol (MD = -0.50; 95% CI -0.86 to -0.13; p < 0.01) compared with conventional clinical therapy. Most in-dices did not support the use of tai chi over aerobic exercise, except for glycated haemoglobin (HbA1c) (MD = -0.24; 95% CI -0.49 to 0.00; p < 0.01) and high-density lipoprotein (MD = 0.07; 95% CI 0.01 to 0.12; p < 0.01).

Conclusion: Tai chi had better effects on metabolic control and body composition indicators than clinical conventional therapy, but only on HbA1c and HDL were superior than that of aerobic exercise. The best time-window for tai chi intervention may differ with different metabolic indices.
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http://dx.doi.org/10.2340/16501977-2799DOI Listing
March 2021

Effect of Tai Chi on Quality of Life, Body Mass Index, and Waist-Hip Ratio in Patients With Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis.

Front Endocrinol (Lausanne) 2020 19;11:543627. Epub 2021 Jan 19.

College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.

Background: Type 2 diabetes mellitus (T2DM) is a worldwide public health concern with high morbidity and various progressive diabetes complications that result in serious economic expenditure and social burden. This systematic review aims to evaluate the effect of Tai Chi on improving quality of life (QoL), body mass index (BMI) and waist-hip ratio (WHR) in patients with T2DM.

Method: A systematic review and meta-analysis was performed following PRISMA recommendation. Four English databases and three Chinese databases were searched. The PEDro scale was used to assess the methodological quality of including studies. Study inclusion criteria: randomized controlled trials (RCTs) and quasi-experimental studies were included, patients with T2DM that adopted Tai Chi as intervention and QoL, BMI and/or WHR as outcome measurements.

Results: Eighteen trials were included. The aggregated results of seven trials showed that Tai Chi statistically significantly improved QoL measured by the SF-36 on every domains (physical function: MD = 7.73, 95% confidence interval (CI) = 1.76 to 13.71, p = 0.01; role-physical function: MD = 9.76, 95% CI = 6.05 to 13.47, p < 0.001; body pain: MD = 8.49, 95% CI = 1.18 to 15.8, p = 0.02; general health: MD = 9.80, 95% CI = 5.77 to 13.82, p < 0.001; vitality: MD = 6.70, 95% CI = 0.45 to 12.94, p = 0.04; social function: MD = 9.1, 95% CI = 4.75 to 13.45, p < 0.001; role-emotional function: MD = 7.88, 95% CI = 4.03 to 11.72, p < 0.001; mental health: MD = 5.62, 95% CI = 1.57 to 9.67, p = 0.006) and BMI (MD = -1.53, 95% CI = -2.71 to -0.36, p < 0.001) compared with control group (wait list; no intervention; usual care; sham exercise).

Conclusion: Tai Chi could improve QoL and decrease BMI for patients with T2DM, more studies are needed to be conducted in accordance with suggestions mentioned in this review.
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http://dx.doi.org/10.3389/fendo.2020.543627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851054PMC
May 2021

Metabolic footprint analysis of volatile metabolites to discriminate between different key time points in the fermentation and storage of starter cultures and probiotic Lactobacillus casei Zhang milk.

J Dairy Sci 2021 Mar 15;104(3):2553-2563. Epub 2021 Jan 15.

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address:

Interest has been growing in the co-fermentation of starter cultures with probiotic bacteria in milk. However, the representative metabolites and metabolic changes at different key time points during milk fermentation and storage in starter cultures and probiotic bacteria are still unclear. In this study, we used gas chromatography/mass spectrometry-based metabolomics to identify volatile metabolites and discriminate between 6 different time points [fermentation initiation (FI), fermentation curd (FC), fermentation termination (FT), storage 1 d (S1d), storage 7 d (S7d), and storage 14 d (S14d)] during the fermentation and storage of starter cultures and Lactobacillus casei Zhang milk. Of the 52 volatile metabolites identified, 15 contributed to discrimination of the 6 time points. Then, using the profile from the different time points, we analyzed pairwise comparisons (FI vs. FC; FC vs. FT; FT vs. S1d; S1d vs. S7d; S7d vs. S14d); these time-lapse comparisons showed metabolic progressions from one fermentation stage to the next. We found representative and exclusive metabolites at specific fermentation and storage time points. The greatest difference in metabolites occurred between FC and FT, and the metabolic profiles between S7d and S14d were most similar. Interestingly, decanoic acid, octanoic acid, and hexanoic acid reached their highest level at storage 14 d, indicating that the post-fermentation storage of fermented milk with L. casei Zhang may add more probiotic functions. This work provides detailed insight into the time-specific profiles of volatile metabolites and their dynamic changes; these data may be used for understanding and eventually predicting metabolic changes in milk fermentation and storage, where probiotic strains may be used.
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http://dx.doi.org/10.3168/jds.2020-18968DOI Listing
March 2021

On-chip programmable optical switch based on CdS multibranched nanowire arrays.

Opt Lett 2021 Jan;46(2):294-297

Micro/nano optoelectronic devices are widely studied as basic building blocks for on-chip integrated microsystem and multichannel logic units with excellent optoelectronic properties that are especially important part for interconnection route construction. Here, based on anisotropic waveguides, an optical switch with an on/off ratio of 2.14 is built up in a 2D CdS branched nanowire array. Because the branches are obliquely distributed at the same side of the trunk in a highly ordered form, the guided photoluminescence (PL) intensity from the trunk into the branch tightly relates to its angle. Based on the different intensity of the guided PL emitted from the end of each branch, the position of the incident spot in the backbone area can be identified accurately, making a feasible construction of an on-chip position-sensitive detector to realize an all-optical information process.
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http://dx.doi.org/10.1364/OL.412587DOI Listing
January 2021

Kinematic dexterity analysis of human-robot interaction of an upper limb rehabilitation robot.

Technol Health Care 2020 Dec 31. Epub 2020 Dec 31.

Background: The definition of rehabilitation training trajectory is of great significance during rehabilitation training, and the dexterity of human-robot interaction motion provides a basis for selecting the trajectory of interaction motion.

Objective: Aimed at the kinematic dexterity of human-robot interaction, a velocity manipulability ellipsoid intersection volume (VMEIV) index is proposed for analysis, and the dexterity distribution cloud map is obtained with the human-robot cooperation space.

Method: Firstly, the motion constraint equation of human-robot interaction is established, and the Jacobian matrix is obtained based on the speed of connecting rod. Then, the Monte Carlo method and the cell body segmentation method are used to obtain the collaborative space of human-robot interaction, and the VMEIV of human-robot interaction is solved in the cooperation space. Finally, taking the upper limb rehabilitation robot as the research object, the dexterity analysis of human-robot interaction is carried out by using the index of the approximate volume of the VMEIV.

Results: The results of the simulation and experiment have a certain consistency, which indicates that the VMEIV index is effective as an index of human-robot interaction kinematic dexterity.

Conclusions: The VMEIV index can measure the kinematic dexterity of human-robot interaction, and provide a reference for the training trajectory selection of rehabilitation robot.
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http://dx.doi.org/10.3233/THC-202633DOI Listing
December 2020

The prevalence of mild cognitive impairment in type 2 diabetes mellitus patients: a systematic review and meta-analysis.

Acta Diabetol 2021 Jun 8;58(6):671-685. Epub 2021 Jan 8.

College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.

Aims: Mild Cognitive impairment (MCI) is common in type 2 diabetes mellitus (T2DM) patients. The impaired cognitive function had harmful effect on patients' diabetic conditions. This study aimed to estimate the prevalence of MCI in T2DM (T2DM-MCI) patients by conducting a systematic review and meta-analysis of observational studies.

Methods: We carried out a literature search until June 1, 2020, for all observational studies in the following databases: Medline (PubMed), Web of Science, and Embase. Two independent reviewers initially screened the eligible articles. Then, a meta-analysis (random effects model) was conducted to estimate the prevalence of MCI in people with T2DM with STATA 16.

Results: A total of 1808 articles were first considered after reading title and abstract, 12 of which remained after reviewing the full text. The combined prevalence of MCI in T2DM patients was estimated to be 45.0% (95% CI=36.0, 54.0). There was no significant heterogeneity through meta-regression and sensitivity analysis. Overall, Europe (n=2, r=36.6%, 95% CI=26.3, 46.9, I=82.3%) had a lower prevalence than Asia (n=10, r=46.4%, 95% CI=36.2, 56.6, I=98%). The overall prevalence in female patients (n=14, r=46.9%, 95% CI=34, 59.8, I=98.3%) was higher than that in male patients (n=14, r=38.8%, 95% CI=27, 50.7, I=98%). Subgroup analysis based on age demonstrated a lower prevalence in patients older than 60 years (n=9, r=44.3%, 95% CI=33.1, 55.6, I=98.3%) than patients younger than 60 years (n=3, r=46.4%, 95% CI=33.3, 59.5, I=91.2%).

Conclusion: Our results demonstrate that the pooled estimated prevalence of mild cognitive impairment in type 2 diabetes mellitus patients is high worldwide, especially in China Asia. Primary care clinicians should pay more attention to the cognitive function of T2DM patients, as mild cognitive impairment is one of the risk factors for Alzheimer's disease.
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http://dx.doi.org/10.1007/s00592-020-01648-9DOI Listing
June 2021

Endometrial extracellular matrix rigidity and IFNτ ensure the establishment of early pregnancy through activation of YAP.

Cell Prolif 2021 Feb 4;54(2):e12976. Epub 2021 Jan 4.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Background: In mammals, early pregnancy is a critical vulnerable period during which complications may arise, including pregnancy failure. Establishment of a maternal endometrial acceptance phenotype is a prerequisite for semiheterogeneous embryo implantation, comprising the rate-limiting step of early pregnancy.

Methods: Confocal fluorescence, immunohistochemistry and western blot for nuclear and cytoplasmic protein were used to examine the activation of yes-associated protein (YAP) in uterine tissue and primary endometrial cells. The target binding between miR16a and YAP was verified by dual-luciferase reporter gene assay. The mouse pregnancy model and pseudopregnancy model were used to investigate the role of YAP in the maternal uterus during early pregnancy in vivo.

Results: We showed that YAP translocates into the nucleus in the endometrium of cattle and mice during early pregnancy. Mechanistically, YAP acts as a mediator of ECM rigidity and cell density, which requires the actomyosin cytoskeleton and is partially dependent on the Hippo pathway. Furthermore, we found that the soluble factor IFNτ, which is a ruminant pregnancy recognition factor, also induced activation of YAP by reducing the expression of miR-16a.

Conclusions: This study revealed that activation of YAP is necessary for early pregnancy in bovines because it induced cell proliferation and established an immunosuppressive local environment that allowed conceptus implantation into the uterine epithelium.
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http://dx.doi.org/10.1111/cpr.12976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849163PMC
February 2021

Rare earth ions-enhanced gold nanoclusters as fluorescent sensor array for the detection and discrimination of phosphate anions.

Chem Asian J 2021 Feb 7;16(3):247-251. Epub 2021 Jan 7.

State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 211198, P. R. China.

The discrimination and detection of phosphate anions have attracted extensive attention due to their important roles in various biological processes. Compared with sensors to detect one individual phosphate at a time, sensor arrays are able to discriminate multiple phosphates simultaneously. In this study, we developed a rare earth ions enhanced AuNCs-based sensor array to achieve facile and rapid identification of phosphate anions (PPi, ADP and ATP). The rare earth ions (i. e., Ce , Gd , Tm and Yb ) can significantly enhance the fluorescence of AuNCs through aggregation-induced emission effect. And the subsequent addition of phosphate anions can recover the fluorescence of the AuNCs-rare earth ions assembly. Thanks to the different numbers of phosphate group and different steric hindrance effects of phosphate anions, the recovery fluorescence of AuNCs-rare earth ions assembly induced by PPi, ADP or ATP are respectively distinct. Thus the sensor array composed of AuNCs and different rare earth ions is able to distinguish those phosphate anions. Finally, the sensor array was successfully demonstrated to identify the phosphates in blind samples.
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http://dx.doi.org/10.1002/asia.202001296DOI Listing
February 2021

River water intrusion as a source of inflow into the sanitary sewer system.

Water Sci Technol 2020 Dec;82(11):2472-2481

Power Construction Corporation of China, Hangzhou, China, 310014.

Previous studies on the extraneous water problem (or infiltration/inflow) in sanitary sewer systems assumed that the wastewater flow is mainly composed of foul sewage (FS), groundwater infiltration (GWI) and rainfall-derived inflow and infiltration (RDII). Most existing assessment methods are based on this assumption. In 2018, China initiated the 'Protection of the Yangtze River Program', and the two-year research data showed that it was neither the GWI nor the RDII but the direct surface water intrusion (DSWI), which has rarely been reported in literatures, that serves as the main source of the extraneous water in many local sewer systems. The discovery has enriched the understanding of the extraneous water in sewer systems. Meanwhile, it brings new challenges for the assessment of extraneous water. In this study, starting from the analysis of the low influent concentration of chemical oxygen demand (COD) of the wastewater treatment plant in a southeastern city in China, a river water intrusion point was successfully localized and the volume of river water intrusion was quantified by a series of field experiments. The methodology used in this study can also be applied in other areas with DSWI.
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http://dx.doi.org/10.2166/wst.2020.516DOI Listing
December 2020