Publications by authors named "ShuJie Zhang"

72 Publications

CNV profiles of Chinese pediatric patients with developmental disorders.

Genet Med 2021 Jan 5. Epub 2021 Jan 5.

Department of Genetics, Capital Institute of Pediatrics, Beijing, China.

Purpose: To examine the overall genomic copy-number variant (CNV) landscape of Chinese pediatric patients with developmental disorders.

Methods: De-identified chromosomal microarray (CMA) data from 10,026 pediatric patients with developmental disorders were collected for re-evaluating the pathogenic CNV (pCNV) yields of different medical conditions and for comparing the frequency and phenotypic variability of genomic disorders between the Chinese and Western patient populations.

Results: The overall yield of pCNVs in the Chinese pediatric patient cohort was 21.37%, with variable yields for different disorders. Yields of pCNVs were positively associated with phenotypic complexity and intellectual disability/developmental delay (ID/DD) comorbidity for most disorders. The genomic burden and pCNV yield in neurodevelopmental disorders supported a female protective effect. However, the stratification analysis revealed that it was seen only in nonsyndromic ID/DD, not in nonsyndromic autism spectrum disorders or seizure. Furthermore, 15 known genomic disorders showed significantly different frequencies in Chinese and Western patient cohorts, and profiles of referred clinical features for 15 known genomic disorders were also significantly different in the two cohorts.

Conclusion: We defined the pCNV yields and profiles of the Chinese pediatric patients with different medical conditions and uncovered differences in the frequency and phenotypic diversity of genomic disorders between Chinese and Western patients.
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http://dx.doi.org/10.1038/s41436-020-01048-yDOI Listing
January 2021

Synthesis, characteristics and medical applications of plant nanomaterials.

Planta 2020 Nov 20;252(6):108. Epub 2020 Nov 20.

School of Public Health, Weifang Medical University, Weifang, 261053, People's Republic of China.

Main Conclusion: The recent preparations of metal nanoparticles using plant extracts as reducing agents are summarized here. The synthesis and characterization of plant-metal nanomaterials and the progress in antibacterial and anti-inflammatory medical applications are detailed, providing a new vision for plant-based medical applications. The medical application of plant-metal nanoparticles is becoming a research hotspot. Compared with traditional preparation methods, the synthesis of plant-metal nanoparticles is less toxic and more eco-friendly, increasing application potential. Highly efficient plant-metal nanoparticles are usually smaller than 100 nm. This review describes the synthesis, characterization and bioactivities of gold- and silver-plant nanoparticles as examples and clearly explained their antibacterial and anticancer mechanisms. An analysis of actual cases shows that the synthetic method and type of plant extract affect the activities of the products.
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http://dx.doi.org/10.1007/s00425-020-03509-9DOI Listing
November 2020

Dimensionality Reduction for Tensor Data Based on Local Decision Margin Maximization.

IEEE Trans Image Process 2021 18;30:234-248. Epub 2020 Nov 18.

In machine learning, the idea of maximizing the margin between two classes is widely used in classifier design. Enlighted by the idea, this paper proposes a novel supervised dimensionality reduction method for tensor data based on local decision margin maximization. The proposed method seeks to preserve and protect the local discriminant information of the original data in the low-dimensional data space. Firstly, we depart the original tensor dataset into overlapped localities with discriminant information. Then, we extract the similarity and anti-similarity coefficients of each high-dimensional locality and preserve these coefficients in the embedding data space via the multilinear projection scheme. Under the combined effect of these coefficients, each dimension-reduced locality tends to be a convex set where strongly correlated intraclass points gather. Simultaneously, the local decision margin, which is defined as the shortest distance from the boundary of each locality to the nearest point of each side, will be maximized. Therefore, the local discriminant structure of the original data could be well maintained in the low-dimensional data space. Moreover, a simple iterative scheme is proposed to solve the final optimization problem. Finally, the experiment results on 6 real-world datasets demonstrate the effectiveness of the proposed method.
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http://dx.doi.org/10.1109/TIP.2020.3034498DOI Listing
November 2020

DCZ3301, an aryl-guanidino agent, inhibits ocular neovascularization via PI3K/AKT and ERK1/2 signaling pathways.

Exp Eye Res 2020 12 26;201:108267. Epub 2020 Sep 26.

Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, And Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, China. Electronic address:

Neovascularization is a critical process in the pathophysiology of neovascular eye diseases. Although anti-VEGF therapy has achieved remarkable curative effects, complications, limited efficacy and drug resistance remain the prominent problems. DCZ3301, an aryl-guanidino compound, was reported to have anti-tumor activity in the previous studies. Here, we demonstrated the effects of DCZ3301 on human umbilical vein endothelial cell (HUVEC) in vitro, and performed choroid microvascular sprouting assay ex vivo and alkali-burn induced corneal neovascularization mouse model in vivo. We found that DCZ3301 inhibited the proliferation, migration, and tube formation of HUVECs, while inducing the spontaneous apoptosis of HUVECs by suppressing the activation of PI3K/AKT and ERK1/2 pathways. Furthermore, DCZ3301 inhibited the choroid microvascular sprouting, diminished the area of corneal neovascularization and attenuated the edema of corneal stroma after alkali burn. Together, these results suggested that DCZ3301 exerted anti-angiogenic properties, and might be regarded as a potential candidate for ocular neovascularization.
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http://dx.doi.org/10.1016/j.exer.2020.108267DOI Listing
December 2020

Identification of differentially expressed long non-coding RNAs and mRNAs in orbital adipose/connective tissue of thyroid-associated ophthalmopathy.

Genomics 2021 Jan 9;113(1 Pt 2):440-449. Epub 2020 Sep 9.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, 83 Fenyang Road, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, 83 Fenyang Road, Shanghai 200031, China. Electronic address:

Extracellular matrix remodeling and orbital adipose/connective tissue expansion are two key features of thyroid-associated ophthalmopathy (TAO). Recent studies have indicated the critical role of long non-coding RNAs (lncRNAs) in the pathogenesis of ocular disorders. However, little is known about the roles of lncRNAs in orbital adipose/connective tissue of TAO. In this study, the profiles of lncRNAs and mRNAs in the orbital adipose/connective tissue of TAO were identified by RNA sequencing. A total of 809 differential lncRNAs and 607 differential mRNAs were identified, among which 52 genes were found to be significantly related to the extracellular matrix. Co-expression network analysis suggested that lncRNAs might regulate extracellular matrix remodeling in orbital adipose/connective tissue of TAO. Additionally, the target genes of lncRNAs involved in the lipid metabolism and cytokine-cytokine receptor interaction were also identified. These results may provide potential regulatory mechanisms of lncRNAs in the orbital adipose/connective tissue of TAO.
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http://dx.doi.org/10.1016/j.ygeno.2020.09.001DOI Listing
January 2021

Multivariate similarity clustering analysis: a new method regarding biogeography and its application in global insects.

Integr Zool 2020 Aug 21. Epub 2020 Aug 21.

Institute of Biodiversity and Ecology, Zhengzhou University, Zhengzhou, China.

A new method, multivariate similarity clustering analysis (MSCA) method, was established for biogeographical distribution analyzing. General similarity formula (GSF), the core of MSCA method, can be used to calculate the similarity coefficients between 2 and among any ≥ 3 geographical units. Taking the global insects as example, we introduced the steps to use of GSF and consequent clustering processes of this method in details. Firstly, geographical distributions of certain taxa (e.g. Insecta) were categorized into basic geographical units (BGUs); Secondly, similarity coefficients between 2 and among n BGUs were calculated using GSF. Thirdly, hierarchical clustering was conducted according to values of similarity coefficients (from high to low); then a clustering diagram was generated. Finally, a framework of biogeographical division map was established for the target taxa (e.g. Insecta). We concluded that the MSCA method was efficiently applied in analyzing the biogeographical distribution of given biological taxa; the geographical regions regarding global insects were categorized into 7 Realms with 20 sub-Realms based on the results of MSCA method.
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http://dx.doi.org/10.1111/1749-4877.12485DOI Listing
August 2020

Author Correction: NH-N alleviates iron deficiency in rice seedlings under calcareous conditions.

Sci Rep 2020 Jul 17;10(1):12215. Epub 2020 Jul 17.

Key Lab of Oasis Ecology Agriculture of Xinjiang Production and Construction Group, Shihezi University, North 4th Street No. 221, Shihezi, 832000, P.R. China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-69146-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368003PMC
July 2020

Disrupted neural signals in patients with concomitant exotropia.

Ophthalmic Physiol Opt 2020 09 16;40(5):650-659. Epub 2020 Jul 16.

Eye Research Institute and Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Purpose: Decreased binocular and oculomotor function in strabismics has recently been considered as cortical in origin. This study aimed to investigate functional abnormalities using a frequency-specific neuroimaging method in patients with concomitant exotropia (XT), and to demonstrate the clinical implications.

Methods: Resting-state functional magnetic resonance imaging data were collected in 26 XT patients and 26 matched controls. To evaluate the local spontaneous neural activity, the amplitude of low frequency fluctuations (ALFF) was calculated in the typical frequency band (0.01-0.08 Hz) as well as five narrowly-defined frequency bands (slow-6: 0-0.01 Hz, slow-5: 0.01-0.027 Hz, slow-4: 0.027-0.073 Hz, slow-3: 0.073-0.167 Hz, and slow-2: 0.167-0.25 Hz), respectively.

Results: Patients with XT showed decreased ALFF in the bilateral parieto-occipital sulcus (POS), and increased ALFF in the bilateral thalamus within the typical frequency band. Frequency-dependent ALFF alterations were found in the higher visual areas such as the right lateral occipital complex (LOC). Furthermore, ALFF in the right LOC in the slow-5 band was positively correlated with fusion control score (r = 0.70, p < 0.0001) and binocular function score (r = 0.67, p = 0.0002). Regression analyses showed that early age of onset remained the only significant explanatory factor for ALFF reduction in the right POS in the typically-measured frequency band (also referred to as the typical frequency band) (Odds ratio, 0.038; 95% confidence interval, 0.001 to 0.075).

Conclusions: Our findings provide spatial information regarding the functionally disrupted regions in XT. Moreover, the frequency-dependent ALLF alteration in the right LOC might reflect a potential plastic capacity in binocular function, which could be a potential objective index for evaluating disease severity.
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http://dx.doi.org/10.1111/opo.12715DOI Listing
September 2020

How CEO Ethical Leadership Influences Top Management Team Creativity: Evidence From China.

Front Psychol 2020 21;11:748. Epub 2020 May 21.

School of Management, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.

The creative thinking and ability of top management team (TMT) members is important in coping with rapid changes in the external environment and improving the competitive advantage of an organization. This research focuses on the CEO-TMT interface to explain how CEOs influence TMT characteristics, which in turn affects TMT outcomes. Based on social learning theory, this study examines the associations among CEO ethical leadership, TMT cohesion, and TMT creativity in a Chinese context using a total of 91 TMTs. To verify the reliability and validity of the constructs, a series of confirmatory factor analyses (CFAs) were run. The results showed that the hypothetical model captured distinct constructs and fits the data well. A multistep regression method was used to test hypotheses. The results indicated that: (a) CEO ethical leadership has a positive effect on TMT creativity; (b) TMT cohesion plays a mediating role in the relationship between CEO ethical leadership and TMT creativity; and (c) power distance plays a moderating role in the relationship between CEO ethical leadership and TMT creativity. The greater the power distance, the weaker the positive relationship between CEO ethical leadership and TMT creativity. This study demonstrates the value of CEO ethical leadership and advocates the importance of establishing team cohesion and building a psychologically safe environment to motivate top managers within an organization to share information and knowledge to improve creativity.
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http://dx.doi.org/10.3389/fpsyg.2020.00748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253678PMC
May 2020

Non-destructive analysis of germination percentage, germination energy and simple vigour index on wheat seeds during storage by Vis/NIR and SWIR hyperspectral imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Oct 16;239:118488. Epub 2020 May 16.

Department of Plant Genetics and Breeding, College of Agriculture, China Agricultural University/Beijing Key Laboratory of Crop Genetic Improvement/The Innovation Center (Beijing) of Crop Seed Sciences Ministry of Agriculture, Beijing 100193, China. Electronic address:

Two hyperspectral imaging (HSI) systems, visible/near infrared (Vis/NIR, 304-1082 nm) and short wave infrared (SWIR, 930-2548 nm), were used for the first time to comprehensively predict the changes in quality of wheat seeds based on three vigour parameters: germination percentage (GP, reflecting the number of germinated seedling), germination energy (GE, reflecting the speed and uniformity of seedling emergence), and simple vigour index (SVI, reflecting germination percentage and seedling weight). Each sample contained a small number of wheat seeds, which were obtained by high temperature and humidity-accelerated aging (0, 2, and 3 days) to simulate storage. The spectra of these samples were collected using HSI systems. After collection, each seed sample underwent a standard germination test to determine their GP, GE, and SVI. Then, several pretreatment methods and the partial least-squares regression algorithm (PLS-R) were used to establish quantitative models. The models for the Vis/NIR region obtained excellent performance, and most effective wavelengths (EWs) were selected in the Vis/NIR region by the successive projections algorithm (SPA) and regression coefficients (RC). Subsequently, PLS-R-RC models using selected wavebands (sixteen wavebands for GP, 14 wavebands for GE, and 16 wavebands for SVI) exhibited similar performance to the PLS-R models based on the full wavebands. The best R results obtained in the simplified models' prediction sets were 0.921, 0.907, and 0.886, with RMSE values of 4.113%, 5.137%, and 0.024, for GP, GE, and SVI, respectively. Distribution maps of GP, GE, and SVI were produced by applying these simplified PLS models. By interpreting the EWs and building prediction models, soluble protein and sugar content were demonstrated to have a relationship with spectral information. In summary, the present results lay a foundation towards the development of a significantly simpler, more comprehensive, and non-destructive hyperspectral-based sorting system for determining the vigour of wheat seeds.
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http://dx.doi.org/10.1016/j.saa.2020.118488DOI Listing
October 2020

A novel pathogenic frameshift variant unmasked by a large de novo deletion at 13q21.33-q31.1 in a Chinese patient with neuronal ceroid lipofuscinosis type 5.

BMC Med Genet 2020 05 11;21(1):100. Epub 2020 May 11.

Genetic and Metabolic Central Laboratory, Birth Defect Prevention Research Institute, Maternal and Child Health Hospital, Children's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530002, China.

Background: Neuronal ceroid lipofuscinosis type 5 (CLN5) is a rare form of neuronal ceroid lipofuscinoses (NCLs) which are a group of inherited neurodegenerative diseases characterized by progressive intellectual and motor deterioration, visual failure, seizures, behavioral changes and premature death. CLN5 was initially named Finnish variant late infantile NCL, it is now known to be present in other ethnic populations and with variable age of onset. Few CLN5 patients had been reported in Chinese population.

Case Presentation: In this paper, we report the symptoms of a Chinese patient who suffer from developmental regression and grand mal epilepsy for several years. The DNA was extracted from peripheral blood of proband and both parents, and then whole exome sequencing was performed using genomic DNA. Both sequence variants and copy number variants (CNVs) were analyzed and classified according to guidelines. As the result, a novel frameshift mutation c.718_719delAT/p.Met240fs in CLN5 and a de novo large deletion at 13q21.33-q31.1 which unmasked the frameshift mutation were identified in the proband. Despite the large de novo deletion, which can be classified as a pathogenic copy number variant (CNV), the patient's clinical presentation is mostly consistent with that of CLN5, except for early developmental delay which is believed due to the large deletion. Both variants were detected simultaneously by exome sequencing.

Conclusions: This is the first report of whole gene deletion in combination with a novel pathogenic sequence variant in a CLN5 patient. The two mutations detected with whole exome sequencing simultaneously proved the advantage of the sequencing technology for genetic diagnostics.
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http://dx.doi.org/10.1186/s12881-020-01039-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216669PMC
May 2020

Genetic Alterations and Checkpoint Expression: Mechanisms and Models for Drug Discovery.

Adv Exp Med Biol 2020 ;1248:227-250

The State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Department of Rheumatology and Immunology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Model Animal Research Center of Nanjing University, Nanjing, Jiangsu, 210061, China.

In this chapter, we will sketch a story that begins with the breakdown of chromosome homeostasis and genomic stability. Genomic alterations may render tumor cells eternal life at the expense of immunogenicity. Although antitumor immunity can be primed through neoantigens or inflammatory signals, tumor cells have evolved countermeasures to evade immune surveillance and strike back by modulating immune checkpoint related pathways. At present, monoclonal antibody drugs targeting checkpoints like PD-1 and CTLA-4 have significantly prolonged the survival of a variety of cancer patients, and thus have marked a great achievement in the history of antitumor therapy. Nevertheless, this is not the end of the story. As the relationship between genomic alteration and checkpoint expression is being delineated though the advances of preclinical animal models and emerging technologies, novel checkpoint targets are on the way to be discovered.
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http://dx.doi.org/10.1007/978-981-15-3266-5_10DOI Listing
April 2020

Novel compound heterozygous pathogenic variants in ASCC1 in a Chinese patient with spinal muscular atrophy with congenital bone fractures 2 : Evidence supporting a "Definitive" gene-disease relationship.

Mol Genet Genomic Med 2020 05 11;8(5):e1212. Epub 2020 Mar 11.

Genetic and Metabolic Central Laboratory, Birth Defect Prevention Research Institute, Maternal and Child Health Hospital, Children's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.

Background: A very limited spectrum of ASCC1 pathogenic variants had been reported in six (mostly consanguineous) families with spinal muscular atrophy with congenital bone fractures 2 [OMIM #616867] since 2016.

Methods: A proband from a non-consanguineous Chinese family presented with neonatal severe hypotonia, respiratory distress, muscle weakness, and atrophy, as well as congenital bone fractures was performed by exome sequencing.

Results: A compound heterozygosity of a nonsense (c.932C>G,p.Ser311Ter) and an exon 5 deletion in ASCC1 segregating with phenotypes was detected, both variants are novel and pathogenic. Since ASCC1 is a relatively new disease gene, we performed the gene curation by ClinGen SOP. The existing evidence is sufficient to support a "Definitive" level of disease-gene relationship.

Conclusion: This case report expended the mutation spectrum of ASCC1 and support the notion that this novel disease also occurs in outbreed populations and this is a rare disease but may still be underdiagnosed due to its perinatal lethal outcomes.
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http://dx.doi.org/10.1002/mgg3.1212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216800PMC
May 2020

BaGeO: A Mid-IR Transparent Crystal with Superstrong Raman Response.

Inorg Chem 2020 Mar 25;59(6):3542-3545. Epub 2020 Feb 25.

Key Laboratory of Functional Materials and Devices for Special Environments, Xinjiang Technical Institute of Physics & Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.

Stimulated Raman scattering processes based on Raman crystals offer a simple and effective method to generate mid-IR lasers. However, currently available mid-IR Raman crystals are extremely scarce. Herein, a new type of mid-IR Raman crystal, BaGeO, is reported. It crystallizes in the monoclinic space group 2/ with Ba cations and [GeO] rings as basic building units and features high transparency from 2.5 to 5.5 μm and a Raman response larger than that of diamond. The BaGeO crystal has 45 IR-active modes (22A + 23B) and 42 Raman-active modes (20A + 22B). The wide mid-IR transparent window is attributed to the low phonon energy of the second-order IR-active A ⊗ B mode. The strongest Raman band, located at 799 cm, arises from the symmetrically stretching vibration of the [GeO] extra-ring Ge-O bonds. The findings provide new insights into the crystallographic and Raman spectroscopic characteristics of high-performance mid-IR Raman crystals.
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http://dx.doi.org/10.1021/acs.inorgchem.0c00155DOI Listing
March 2020

Integrative transcriptomics and proteomic analysis of extraocular muscles from patients with thyroid-associated ophthalmopathy.

Exp Eye Res 2020 04 10;193:107962. Epub 2020 Feb 10.

Department of Ophthalmology, Eye & ENT Hospital, Shanghai Medical College, Fudan University, 83 Fenyang Road, Shanghai, China; NHC Key Laboratory of Myopia, Fudan University, 83 Fenyang Road, Shanghai, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, 83 Fenyang Road, Shanghai, China. Electronic address:

Our study aimed to reveal the underlying pathologic mechanisms of thyroid-associated ophthalmopathy (TAO) by integrative transcriptomics and proteomic analysis of extraocular muscles (EOM). The study involved 11 TAO patients (clinical activity score ≤ 2) and 11 control donors. Total RNA was extracted from EOM samples of 5 TAO patients and 5 control individuals for gene microarray analysis to reveal differentially expressed genes. Concurrently, EOM samples from 3 TAO patients and 3 control individuals were lysed for quantitative proteomic analysis. Differentially expressed genes and proteins were identified, followed by functional and pathway enrichment analysis and protein-protein interaction network construction. Concordance between proteins and transcripts was examined, and functional annotations were conducted. Expressions of versican (VCAN) and lipocalin 1 (LCN1) in EOM samples from another 3 TAO patients and 3 control individuals were measured by western blotting. In total, 952 genes and 137 proteins were identified as differentially expressed, as well as 96 differentially expressed proteins without significantly changed mRNA abundance. Proteins mainly related to the composition (such as MYH1, MYH2, and MYH13) and contraction force (MYH3, MYH8, ACTN3, and TNNT1) of the muscle fibers were significantly up-regulated in EOM samples of TAO, as well as those (such as VCAN, MPZ, and PTPRC) associated with cell adhesion. In addition, differentially expressed proteins related to the components and metabolism of extracellular matrix (ECM) (such as COL1A1, COL1A2, COL2A1, VCAN, OGN, and DCN) were identified. Similarly, expressions of genes involved in cell adhesion and ECM metabolism were significantly different between EOM samples of TAO patients and controls. Western blotting verified that VCAN involved in ECM proteoglycans and diseases associated with glycosaminoglycan metabolism was markedly higher in EOM samples of TAO, whereas LCN1 was obviously decreased. In conclusion, this study demonstrated the significantly altered cellular components of EOM, muscle contraction, cell adhesion and ECM metabolism, which might be involved in the pathologic mechanisms and/or consequences of TAO.
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http://dx.doi.org/10.1016/j.exer.2020.107962DOI Listing
April 2020

Ultrasensitive and regenerable nanopore sensing based on target induced aptamer dissociation.

Biosens Bioelectron 2020 Mar 8;152:112011. Epub 2020 Jan 8.

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China. Electronic address:

For ionic current rectification (ICR) based sensing, nanopore functionalizations are mostly designed for directly binding target molecules to generate detectable signals from surface charge variation. However, this strategy is highly dependent on the charge difference between the captured molecules and surface functionalization layers, which will increase the nanopore design difficulty and subsequently limit the nanopore applicability. Another key challenge for ICR based sensing is the nanopore regenerability that is critical if online monitoring or repeated determination needs to be performed with one sensor. Though some types of nanopore regeneration have been realized on some specific targets or with harsh conditions, it is still highly favored to develop a regenerability using mild conditions for various targets. To address these two challenges, we developed a novel and universal sensing strategy for aptamer-functionalized nanopore that can be easily regenerated after each usage without any harsh conditions and independent of target molecule charge or size for ICR based nanopore sensing. Ochratoxin A (OTA) was used as a model analyte and its corresponding aptamer partially hybridized with the pre-immobilized complementary DNA (cDNA) onto the nanopore inner surface. We demonstrated that the recognition and conjugation of OTA with its aptamer resulted in rectified ionic current variations due to the dissociation between the OTA aptamer and its partially paired cDNA. The performance of this nanopore sensor including sensitivity, selectivity, regenerability, and applicability was characterized using rectified ionic current. This nanopore sensing strategy will provide a promising platform for extensive targets and online sensing applications.
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http://dx.doi.org/10.1016/j.bios.2020.112011DOI Listing
March 2020

NH-N alleviates iron deficiency in rice seedlings under calcareous conditions.

Sci Rep 2019 09 3;9(1):12712. Epub 2019 Sep 3.

Key Lab of Oasis Ecology Agriculture of Xinjiang Production and Construction Group, Shihezi University, North 4th Street No. 221, Shihezi, 832000, P.R. China.

Drip-irrigated rice (Oryza sativa L.) in calcareous soil exhibits signs of iron (Fe) deficiency. This study aimed to explore whether NH alleviates Fe deficiency in rice seedlings grown under calcareous conditions. Two rice varieties (cv. 'T43' Fe deficiency-tolerant variety and cv. 'T04' Fe deficiency-sensitive variety) were used to carry out two independent experiments with exposure to different nitrogen (N) forms (nitrate (NO) or NH) under calcareous conditions. In experiment 1, plants were precultured in a nutrient solution with excess Fe (40 µM Fe(II)-EDTA) for 14 d and then supplied NO-N (AN) or NH-N (NN) without Fe for 3, 6, or 12 d. In experiment 2, plants were fed AN or NN with 10 µM Fe(II)-EDTA for 18 d. Compared to plants exposed to AN, leaves of plants exposed to NN showed severe chlorosis and significantly decreased chlorophyll content during Fe starvation. The xylem sap pH and cell wall Fe fraction in both shoots and roots of rice fed NN were significantly higher than those fed AN. However, the Fe concentration in xylem sap, soluble and organelle Fe fractions in both shoots and roots, and the shoot/root Fe content ratio in rice exposed to AN were significantly higher than those in plants exposed to NN. AN reduced the root aerenchyma fraction and root porosity compared to NN, which induced greater water uptake and hydraulic conductance by roots, hence the stronger xylem sap flow rate with AN. The results indicated that NH-N alleviated Fe deficiency in rice under calcareous conditions by promoting Fe re-allocation in rice tissues and Fe transportation from roots to shoots.
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http://dx.doi.org/10.1038/s41598-019-49207-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722072PMC
September 2019

Aflatoxin B removal by three bacterial strains and optimization of fermentation process parameters.

Biotechnol Appl Biochem 2019 Nov 3;66(6):930-938. Epub 2019 Sep 3.

School of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, Henan, People 's Republic of China.

Aflatoxin B (AFB ) removing bacterial strains were isolated from different habitats that were easily contaminated by AFB . Furthermore, the composition of the fermentation medium and conditions of fermentation process were optimized, including carbon source, nitrogen source, metal ions, temperature, initial pH value, inoculation volume, and culture broth volume. Using coumarin as the sole carbon and energy source, we primarily screened 31 strains, and 10 strains were found to be capable to remove AFB . Among them, the highest removal rate of 71.91% appeared in those isolated from rotten wood (poplar). Strains XY1, XY3, and T6 were carried out to identify, and the results were Klebsiella sp., Klebsiella pneumonia, and Pantoea sp., respectively. Corn cob powder and tryptone can significantly increase the AFB removal activity of these strains. The AFB removal activity of Klebsiella sp.XY1 and K. pneumonia XY3 can be enhanced by Ca , and those of Pantoea sp. T6 can be enhanced by Cu . Temperature and initial pH were positively correlated with the AFB removal activity of these strains in a certain range. This study not only provides reference for the screening and application of AFB removing bacteria, but also provides a basis for possible application in the food and feed industry.
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http://dx.doi.org/10.1002/bab.1807DOI Listing
November 2019

Novel genotypes and phenotypes among Chinese patients with Floating-Harbor syndrome.

Orphanet J Rare Dis 2019 06 14;14(1):144. Epub 2019 Jun 14.

Department of Medical Genetics and Molecular Diagnostic Laboratory, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200000, People's Republic of China.

Background: Floating-Harbor syndrome (FHS) is a rare syndromic short stature disorder caused by truncating variants in SRCAP. Few Chinese FHS patients had been reported so far and limited knowledge regarding the benefit of growth hormone treatment existed.

Methods: We ascertained 12 short stature patients with molecularly confirmed diagnosis of FHS by whole exome sequencing. We performed a comprehensive clinical evaluation for all patients and assessed the responsiveness of growth hormone treatment in a subset of the patients.

Results: Five distinct pathogenic/likely pathogenic variants were identified in 12 independent FHS patients including two previously reported variants (c.7303C > T/p.Arg2435Ter and c.7330C > T/p.Arg2444Ter) and three novel variants (c.7189G > T/p.Glu2397Ter, c.7245_7246delAT/p.Ser2416ArgfsTer26 and c.7466C > G/p.Ser2489Ter). The c.7303C > T/p.Arg2435Ter mutation appears more common in Chinese FHS patients. The clinical presentations of Chinese FHS patients are very similar to those of previously reported patients of different ethnicities. Yet we noticed micropenis and ear abnormalities in multiple patients, suggesting that these may be novel phenotypes of Floating-Harbor syndrome. Eight patients (one with GH deficiency, one with undetermined GH level, six without GH deficiency) underwent growth hormone treatment, 3 patients had good responses, one with modest and two with poor responses.

Conclusion: We described novel genotypes and phenotypes in a Chinese FHS patient cohort. We showed that about half of FHS patients exhibited modest to good response to GH treatment regardless of their respective GH deficiency status. We didn't find any correlation between different mutations and response to GH treatment.
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http://dx.doi.org/10.1186/s13023-019-1111-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570847PMC
June 2019

Genetic mapping of a major QTL promoting homoeologous chromosome pairing in a wheat landrace.

Theor Appl Genet 2019 Jul 23;132(7):2155-2166. Epub 2019 Apr 23.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu Campus, Wenjiang, 611130, Sichuan, China.

Key Message: Common wheat landrace Kaixian-luohanmai carries a gene(s) that promotes homoeologous chromosome pairing. A major QTL responsible for this effect was mapped to chromosome arm 3AL. Polyhaploid hybrids of a Chinese common wheat landrace Kaixian-luohanmai (KL) and related species show increased levels of chromosome pairing. Over 90% of that pairing is between homoeologous arms of wheat chromosomes, with a very strong preference for pairing between homoeologs from genomes A and D. Wheat-rye pairing was also observed at low frequency. Two mapping populations were created from the hybrids of KL with two wheat genotypes top crossed to rye. Mean chiasmata numbers per plant were used as phenotypic data. Wheat 660 K and 15 K SNP arrays, DArT markers and SSR markers were used for genotyping of the top-cross ABDR hybrids. One major QTL, named QPh.sicau-3A, for increased homoeologous pairing was detected on chromosome arm 3AL, and it was responsible for ca. 16% of the total variation. This QTL was located in the interval 696-725 Mb in the Chinese Spring reference genome. SNP markers closely linked with QPh.sicau-3A were converted to KASP markers and validated for marker-assisted selection.
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http://dx.doi.org/10.1007/s00122-019-03344-xDOI Listing
July 2019

Soil Enzyme Activity in Soils Subjected to Flooding and the Effect on Nitrogen and Phosphorus Uptake by Oilseed Rape.

Front Plant Sci 2019 27;10:368. Epub 2019 Mar 27.

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China.

Waterlogging presents one of the greatest constraints for agricultural crops. In order to elucidate the influences of waterlogging stress on the growth of oilseed rape, a pot experiment was performed investigating the impact of waterlogging on nitrogen (N) and phosphorus (P) accumulation in oilseed rape, and mineral N and available P profiles and enzyme activities of soils. The experiment included waterlogging treatments lasting 3 (I), 6 (II), and 9 (III) days, and a control treatment without waterlogging (CK). Results showed that waterlogging lasting 3 or more days significantly depressed the growth of oilseed rape, and prolonged the recovery time of plant growth with the period of flooding. Waterlogging notably influenced the N and P concentrations in plant tissues, and also affected mineral N, available P profiles, and activities of enzymes (including urease, phosphatase, invertase, and catalase) in the soils. With the exception of catalase, flooding suppressed the activity of urease, phosphatase, and invertase to varying degrees, and the longer the flooding time, the greater the suppression. The effect of waterlogging on mineral N and P profiles resulted from the altered proportions of NH-N and NO-N, and the decreased available P concentrations in these soils, respectively. The effect on P was more significant than on N in both soil nutrient profile and plant utilization.
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http://dx.doi.org/10.3389/fpls.2019.00368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445880PMC
March 2019

In Situ Raman Spectroscopy and DFT Studies of the LiGeO Melt Structure.

Inorg Chem 2019 Apr 27;58(8):5025-5030. Epub 2019 Mar 27.

School of Material Science and Engineering , Shanghai University , Shanghai 200072 , China.

Knowledge of the molecular-level structure of the LiGeO melt is essential to understand its basic physicochemical properties. In this work, in situ Raman spectroscopy, factor group analysis, and density functional theory (DFT) calculations were applied to investigate the LiGeO crystal Raman spectrum and its transformation during the crystal melting process. Finally, the LiGeO melt structure was determined. The LiGeO lattice phonons were fully analyzed by the factor group. The DFT calculations confirmed the analysis results and assigned all of the experimental Raman bands. There are two characteristic Raman bands in the experimental spectrum. The 495 cm band (mid-frequency band) is attributed to the symmetric bending vibration of the Ge-O-Ge bond, and the 814 cm band (high-frequency band) arises from the symmetric stretching vibration of the O-Ge-O bond. The mid-frequency band anomalously shifted to a higher frequency and the high-frequency band normally shifted to a lower frequency when the crystal melted. The DFT method was employed to investigate two possible LiGeO melt structures, one consisting of the [GeOØ] (Ø = bridging oxygen) chain and the other consisting of the [GeO] ring. The chain-type structure was demonstrated to provide a better description of the LiGeO melt than the ring-type structure. The anomalous shift of the mid-frequency band is related to the shrinkage of the [GeOØ] chain. On the basis of the chain-type structure, the high viscosity of the LiGeO melt and the growth phenomena of the LiGeO crystal were explained.
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http://dx.doi.org/10.1021/acs.inorgchem.9b00051DOI Listing
April 2019

Identification of a Novel 9.7 kb Deletion Causing α-Thalassemia in Two Pregnant Women in Southern China.

Hemoglobin 2018 May;42(3):209-212

a Laboratory of Genetics and Metabolism , Guangxi Zhuang Autonomous Region Women and Children Care Hospital, Xiangzhu , Nanning , Guangxi Zhuang Autonomous Region , People's Republic of China.

The technique of combining multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (CGH) and gap-polymerase chain reaction (gap-PCR) is an effective way to locate unknown breakpoints on the α-globin genes. In the current report, a novel deletion was detected in two pregnant women with moderate hematological phenotypes. Multiplex ligation-dependent probe amplification and array CGH revealed a probable 9.7 kb deletion at 16p13.3. The breakpoints were precisely defined by gap-PCR and direct sequencing. This deletion (NG_000006.1: g.32709_42418del) included HBA1, HBA2 and HBQ, which resulted in an α-thalassemia (α-thal) mutation. There would be a 25.0% chance of conceiving an α-thal intermedia or α-thal major (α-TI or α-TM) fetus if the couples are both carriers. Rare large deletions can cause α-thalassemia (α-thal) and the structure analysis of an unknown deletion is important for clinical diagnosis and further genetic counseling.
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http://dx.doi.org/10.1080/03630269.2018.1516673DOI Listing
May 2018

Purification and characteristics of an aflatoxin B1 degradation enzyme isolated from Pseudomonas aeruginosa.

FEMS Microbiol Lett 2019 03;366(5)

School of Food Science and Technology, Henan University of Technology, 100# Lianhua Street, High-tech Industrial Development Zone, Zhengzhou 450001, Henan, People's Republic of China.

Aflatoxin is a highly toxic mycotoxin produced by Aspergillus flavus and Aspergillusparasiticus fungus. Since it contaminates food and grain widely, it has seriously endanger the health of human beings and animals. Some microorganisms that exist in nature can degrade aflatoxin. In this paper, the biological AFB1 (aflatoxin B1)-degradation by Pseudomonas aeruginosa M19 was evaluated. We found that the culture supernatant of P. aeruginosa M19 added with proteinase K (Prok), SDS and heating significantly decreased degradation capacity. Pseudomonas AFB1-degrading enzyme (PADE) was purified from P. aeruginosa M19 by a three-step procedure including ammonium sulfate fractional precipitation and ion exchange chromatography and gel filtration chromatography. PADE was purified 175-fold with a recovery of 35%, and an ultimate specific activity of 6.1 × 104 U/mg was achieved. The molecular weight of PADE estimated by SDS-PAGE is about 48 kDa. PADE displayed the highest degradation activity for AFB1 at 65°C and pH 6.0. Cu2+ and Fe3+ strongly enhanced the activity, whereas Ca2+ and Zn2+ strongly inhibited the activity. These findings indicate that PADE from P. aeruginosa M19 is a promising candidate for AFB1 biodegradation.
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http://dx.doi.org/10.1093/femsle/fnz034DOI Listing
March 2019

Differential expression of connective tissue growth factor and hepatocyte growth factor in the vitreous of patients with high myopia versus vitreomacular interface disease.

BMC Ophthalmol 2019 Jan 21;19(1):25. Epub 2019 Jan 21.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China.

Background: To determine the levels of connective tissue growth factor (CTGF) and hepatocyte growth factor (HGF) in the vitreous of patients with high myopia, in comparison with those with a vitreomacular interface disease (VMID).

Methods: Patients with either high myopia (high myopia group) or a VMID (VMID group) were included in this study. Each of the two groups were further subdivided into two subgroups: group A (high myopia with macular hole), group B (high myopia with macular retinoschisis), group C (idiopathic macular hole), and group D (idiopathic epiretinal membrane). Vitreal specimens were collected during vitrectomy, and enzyme-linked immunosorbent assay was used to quantitatively measure the CTGF and HGF levels in the vitreous.

Results: The average axial length was markedly longer in the high myopia group than in the VMID group. The vitreal CTGF level was significantly higher in the high myopia group than in the VMID group. Subgroup analysis revealed significantly higher vitreal CTGF in group A than in the other three subgroups. The vitreal HGF level was not significantly different between the high myopia and VMID groups, but was significantly higher in group D than in group C in the subgroup analysis. Correlation analysis showed that the vitreal CTGF level was positively correlated with the axial length.

Conclusions: The vitreal CTGF level is elevated in highly myopic eyes and may be related to the pathogenesis of high myopia, whereas increased expression of HGF may be involved in the development of idiopathic epiretinal membrane.
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http://dx.doi.org/10.1186/s12886-019-1041-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341692PMC
January 2019

IL-17A synergistically enhances TLR3-mediated IL-36γ production by keratinocytes: A potential role in injury-amplified psoriatic inflammation.

Exp Dermatol 2019 03 12;28(3):233-239. Epub 2019 Feb 12.

Department of Dermatology, Shanghai Skin Disease Hospital, Shanghai, China.

Skin injury can trigger formation of new lesions in psoriasis (Koebner phenomenon). The mechanisms through which injury exacerbates psoriasis are unclear. During wound repair, epidermal keratinocytes are activated and produce abundant IL-36γ, further promoting the skin inflammation. IL-17A is the cornerstone cytokine in the pathogenesis of psoriasis. We sought to investigate the effects of IL-17A on injury-induced keratinocyte activation and IL-36γ production. Here, we demonstrated that dsRNA released from necrotic keratinocytes induced the expression of IL-36γ. Silencing of TLR3 by siRNA decreased the IL-36γ induction by necrotic keratinocyte supernatant. Co-stimulation with dsRNA and IL-17A synergistically increased the expression of IL-36γ and other proinflammatory mediators (CCL20, CXCL8, DEFB4 and LCN2) in keratinocytes. The synergistic effects were not dependent on TLR3 upregulation, TNF receptor signalling and mRNA stabilization. Co-stimulation with dsRNA and IL-17A resulted in an accumulation of IκBζ. The synergistic upregulation of IL-36γ and proinflammatory mediators were inhibited by IκBζ siRNA. Co-stimulation with IL-17A and poly(I:C) markedly activated the p38 MAPK and NF-κB pathway, compared with poly(I:C). Blockade of p38 MAPK and NF-κB suppressed dsRNA/IL-17A-mediated IκBζ and IL-36γ induction. These findings demonstrated that IL-17A synergistically enhanced the dsRNA-mediated IL-36γ production through a p38 MAPK-, NF-κB-, and IκBζ-dependent mechanism.
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http://dx.doi.org/10.1111/exd.13871DOI Listing
March 2019

RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice.

Cell Biol Int 2019 Oct 9;43(10):1163-1173. Epub 2019 Aug 9.

Department of Orthopedics, Shandong Energy Zibo Mining Group Co. Ltd. Central Hospital, 133 Zikuang Road, Zichuan District, Zibo, Shandong, 255120, P.R. China.

Cardiac fibrosis is associated with various cardiovascular diseases and can eventually lead to heart failure. Dysregulation of long non-coding RNAs (lncRNAs) are recognized as one of the key mechanisms of cardiac diseases. However, the roles and underlying mechanisms of lncRNAs in cardiac fibrosis have not been explicitly defined. Here, we investigated the role of an antisense (AS) lncRNA from the Ras association domain-containing protein 1 isoform A (RASSF1A) gene locus, named RASSF1-AS1, in the development of cardiac fibrosis. Cardiac fibrosis mouse model was established by isoproterenol injection. We found that RASSF1A protein was downregulated, whereas RASSF1-AS1 was markedly upregulated during cardiac fibrosis. Overexpression and knockdown of mouse primary cardiac fibroblasts showed that RASSF1-AS1 negatively regulated RASSF1A expression at the post-transcriptional level. According to the landscape analysis and sense-AS binding evaluation, RASSF1-AS1 partially overlaps with RASSF1A messenger RNA (mRNA) at the exon2 region. RNA pull-down and luciferase activity assays confirmed that RASSF1-AS1 directly bound to RASSF1A mRNA and suppressed its translation. Furthermore, wild-type RASSF1-AS1 had a promoting effect on nuclear factor-κB activation and cardiac fibrosis, but mutated RASSF1-AS1, in which the binding region was deleted, had no effect. In conclusion, RASSF1-AS1 inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice, indicating a potential application of RASSF1-AS1 as a therapy target for cardiac fibrosis.
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http://dx.doi.org/10.1002/cbin.11085DOI Listing
October 2019

Purification and identification of an aflatoxin B degradation enzyme from Pantoea sp. T6.

Toxicon 2019 Jan 15;157:35-42. Epub 2018 Nov 15.

College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China. Electronic address:

Aflatoxin B (AFB) is a secondary metabolite produced by Aspergillus flavus and other fungi. Soil, crops, food, feed, etc. were susceptible to aflatoxin B contamination, which caused adverse economic and health consequences. It is necessary to search for microorganisms or microbial enzymes that can degrade AFB. The degradation activity of AFB by cell-free supernatant (68.30%) of isolate Pantoea sp. T6 was significantly higher (P < 0.05) than viable bacterial cells (4.87%) and intracellular cell extracts (3.68%). The supernatant's AFB degradation activity was reduced from 68.30% to 5.33% in treatment with protease K and sodium dodecyl sulphate (SDS). An extracellular enzyme from the supernatant was secreted by Pantoea sp. T6 and was named as Pantoea aflatoxin degradation enzyme (PADE), which was obtained using Diethylaminoethanol (DEAE)-Sepharose chromatography. PADE was further purified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The PADE, outer membrane protein A, was identified by mass spectrometry and molecular mass was 38180.1Da. The optimum temperature and pH for the reaction of PADE with AFB were 40 °C and 7.0, respectively. These finding showing that the PADE, which was isolated from the supernatant of Pantoea sp. T6, has the ability to degrade AFB, and may have potential application for aflatoxin B reduction in the food and feed industry.
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http://dx.doi.org/10.1016/j.toxicon.2018.11.290DOI Listing
January 2019

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function.

Cell Physiol Biochem 2018 4;50(1):246-260. Epub 2018 Oct 4.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.

Background/aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation.

Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675.

Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3'UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress.

Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.
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http://dx.doi.org/10.1159/000494003DOI Listing
November 2018

Relationships Between Social Support, Loneliness, and Internet Addiction in Chinese Postsecondary Students: A Longitudinal Cross-Lagged Analysis.

Front Psychol 2018 11;9:1707. Epub 2018 Sep 11.

Department of Psychology, Shandong Normal University, Jinan, China.

Using the Internet has become one of the most popular leisure activities among postsecondary students in China. Concern about the large number of students using the Internet has led to an increase in research on the influencing factors of Internet addiction and the negative consequences caused by it. This short-term longitudinal study examined the associations among three dimensions of social support [objective support (OS), subjective support (SS), and support utilization (SU)], loneliness, and the four dimensions of Internet addiction (compulsive Internet use [CIU] & withdrawal from Internet addiction [WIA], tolerance of Internet addiction [TIA], time-management problems [TMPs], and interpersonal and health problems [IHPs]) in a Chinese sample. A total of 169 postsecondary first-year students (88 girls and 81 boys; mean age = 18.31 years) participated in the study. The questionnaire measurements were taken at the beginning of the school year (T1), 6 months later (T2), and 1 year later (T3). Cross-lagged and structural equation modeling analyses indicated that (a) OS (T1) and SU (T1) negatively predicted loneliness (T2); and loneliness (T2) negatively predicted OS (T3) and SU(T3); (b) CIU & WIA (T1) and TMPs (T1) positively predicted loneliness (T2); and loneliness (T2) positively predicted CIU & WIA (T3), TIA (T3), TMP (T3), and IHP (T3); (c) SS (T1) directly affected TIA (T3) and TMP (T3); and (d) loneliness (T2) played a mediating role in the relationships between OS (T1) and CIU (T3), OS (T1) and TMP (T3), OS (T1) and IHP (T3), and SU (T1) and IHP (T3). Finally, interventions for Internet addiction and implications for future studies were discussed.
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http://dx.doi.org/10.3389/fpsyg.2018.01707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143803PMC
September 2018