Publications by authors named "Shu-Ting Zhang"

29 Publications

  • Page 1 of 1

A Recently Assembled Degradation Pathway for 2,3-Dichloronitrobenzene in sp. Strain JS3051.

mBio 2021 Aug 24;12(4):e0223121. Epub 2021 Aug 24.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

sp. strain JS3051 utilizes 2,3-dichloronitrobenzene (23DCNB), a toxic anthropogenic compound, as the sole carbon, nitrogen, and energy source for growth, but the metabolic pathway and its origins are unknown. Here, we establish that a gene cluster (), encoding a Nag-like dioxygenase, is responsible for the initial oxidation of the 23DCNB molecule. The 2,3-dichloronitrobenzene dioxygenase system (DcbAaAbAcAd) catalyzes conversion of 23DCNB to 3,4-dichlorocatechol (34DCC). Site-directed mutagenesis studies indicated that residue 204 of DcbAc is crucial for the substrate specificity of 23DCNB dioxygenase. The presence of glutamic acid at position 204 of 23DCNB dioxygenase is unique among Nag-like dioxygenases. Genetic, biochemical, and structural evidence indicate that the 23DCNB dioxygenase is more closely related to 2-nitrotoluene dioxygenase from sp. strain JS42 than to the 34DCNB dioxygenase from sp. strain JS3050, which was isolated from the same site as strain JS3051. A gene cluster () encoding the enzymes for 34DCC catabolism, homologous to a operon in Pseudomonas knackmussii strain B13, is also on the chromosome at a distance of 2.5 Mb from the genes. Heterologously expressed DccA catalyzed ring cleavage of 34DCC with high affinity and catalytic efficiency. This work not only establishes the molecular mechanism for 23DCNB mineralization, but also enhances the understanding of the recent evolution of the catabolic pathways for nitroarenes. Because anthropogenic nitroaromatic compounds have entered the biosphere relatively recently, exploration of the recently evolved catabolic pathways can provide clues for adaptive evolutionary mechanisms in bacteria. The concept that nitroarene dioxygenases shared a common ancestor with naphthalene dioxygenase is well established. But their phylogeny and how they evolved in response to novel nitroaromatic compounds are largely unknown. Elucidation of the molecular basis for 23DCNB degradation revealed that the catabolic pathways of two DCNB isomers in different isolates from the same site were derived from different recent origins. Integrating structural models of catalytic subunits and enzymatic activities data provided new insight about how recently modified enzymes were selected depending on the structure of new substrates. This study enhances understanding and prediction of adaptive evolution of catabolic pathways in bacteria in response to new chemicals.
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http://dx.doi.org/10.1128/mBio.02231-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406286PMC
August 2021

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.

J Neuroinflammation 2021 Jul 5;18(1):150. Epub 2021 Jul 5.

Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.

Background: Our recent studies have identified that the red nucleus (RN) dual-directionally modulates the development and maintenance of mononeuropathic pain through secreting proinflammatory and anti-inflammatory cytokines. Here, we further explored the action of red nucleus IL-33 in the early development of mononeuropathic pain.

Methods: In this study, male rats with spared nerve injury (SNI) were used as mononeuropathic pain model. Immunohistochemistry, Western blotting, and behavioral testing were used to assess the expressions, cellular distributions, and actions of red nucleus IL-33 and its related downstream signaling molecules.

Results: IL-33 and its receptor ST2 were constitutively expressed in the RN in naive rats. After SNI, both IL-33 and ST2 were upregulated significantly at 3 days and peaked at 1 week post-injury, especially in RN neurons, oligodendrocytes, and microglia. Blockade of red nucleus IL-33 with anti-IL-33 neutralizing antibody attenuated SNI-induced mononeuropathic pain, while intrarubral administration of exogenous IL-33 evoked mechanical hypersensitivity in naive rats. Red nucleus IL-33 generated an algesic effect in the early development of SNI-induced mononeuropathic pain through activating NF-κB, ERK, p38 MAPK, and JAK2/STAT3, suppression of NF-κB, ERK, p38 MAPK, and JAK2/STAT3 with corresponding inhibitors markedly attenuated SNI-induced mononeuropathic pain or IL-33-evoked mechanical hypersensitivity in naive rats. Red nucleus IL-33 contributed to SNI-induced mononeuropathic pain by stimulating TNF-α expression, which could be abolished by administration of inhibitors against ERK, p38 MAPK, and JAK2/STAT3, but not NF-κB.

Conclusions: These results suggest that red nucleus IL-33 facilitates the early development of mononeuropathic pain through activating NF-κB, ERK, p38 MAPK, and JAK2/STAT3. IL-33 mediates algesic effect partly by inducing TNF-α through activating ERK, p38 MAPK and JAK2/STAT3.
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http://dx.doi.org/10.1186/s12974-021-02198-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258957PMC
July 2021

Mechanisms of neuronal cell death in ischemic stroke and their therapeutic implications.

Med Res Rev 2021 May 6. Epub 2021 May 6.

Department of Neurology and State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Ischemic stroke caused by arterial occlusion is the most common type of stroke, which is among the most frequent causes of disability and death worldwide. Current treatment approaches involve achieving rapid reperfusion either pharmacologically or surgically, both of which are time-sensitive; moreover, blood flow recanalization often causes ischemia/reperfusion injury. However, even though neuroprotective intervention is urgently needed in the event of stroke, the exact mechanisms of neuronal death during ischemic stroke are still unclear, and consequently, the capacity for drug development has remained limited. Multiple cell death pathways are implicated in the pathogenesis of ischemic stroke. Here, we have reviewed these potential neuronal death pathways, including intrinsic and extrinsic apoptosis, necroptosis, autophagy, ferroptosis, parthanatos, phagoptosis, and pyroptosis. We have also reviewed the latest results of pharmacological studies on ischemic stroke and summarized emerging drug targets with a focus on clinical trials. These observations may help to further understand the pathological events in ischemic stroke and bridge the gap between basic and translational research to reveal novel neuroprotective interventions.
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http://dx.doi.org/10.1002/med.21817DOI Listing
May 2021

Association between the Genetic Polymorphisms of , and with Chronic Hepatitis C Virus Infection in the Chinese Han Population.

Immunol Invest 2021 Apr 26:1-16. Epub 2021 Apr 26.

Department of Cell Biology, Dalian Medical University, Dalian, Liaoning, China.

Hepatitis C virus (HCV) infection is a global public health burden. Chronic HCV infection leads to the development of fibrosis, cirrhosis, liver cancer, and liver failure over time. A total of 250 patients with chronic HCV infection and 299 healthy blood donors were recruited. Sixteen candidate single nucleotide polymorphisms (SNPs) in chemokine (C-C motif) ligand 2 (, C-C chemokine receptor 2 (), and were genotyped in all participants. The rs1024610 AA genotype was significantly associated with decreased susceptibility to chronic HCV infection. Aspartate aminotransferase (AST) levels, AST/platelet ratio index, and the fibrosis 4 score were significantly lower in the rs1024610 T allele and haplotype ATGC carriers. Moreover, expression levels of collagen IV (C-IV) and laminin (LN) were significantly higher in patients with the rs2280788 C allele compared to the non-carriers. Similarly, the expression levels of C-IV, LN, and hyaluronic acid were significantly higher in patients with the haplotype, TGCT. No significant differences were identified between the SNPs/haplotypes and plasma levels of , and in the healthy controls, and the rs1024610 allele alteration had no effect on promoter activity. This is the first study to report an association between rs1024610 and the risk of chronic HCV infection in the Chinese Han population. rs1024610 and ATGC haplotype in were reasonable candidate markers of liver abnormalities. rs2280788 and TGCT haplotype in are likely to play a significant role in liver fibrosis during chronic HCV infection.
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http://dx.doi.org/10.1080/08820139.2021.1916524DOI Listing
April 2021

PGC-1α activates SIRT3 to modulate cell proliferation and glycolytic metabolism in breast cancer.

Neoplasma 2021 Mar 25;68(2):352-361. Epub 2020 Nov 25.

School of Mechanical and Material Engineering, North China University of Technology, Beijing, China.

Breast cancer is the leading cause of death among women. PGC-1α plays an important role in the regulation of metabolic reprogramming in cancer cells. SIRT3 has significant implications for tumor growth. In this study, we explored the roles of PGC-1α and SIRT3 in cell proliferation and mitochondrial energy metabolism alterations in breast cancer cells. The expression patterns of PGC-1α and SIRT3 were examined using qRT-PCR and western blotting analysis. MCF-7 and MDA-MB-231 cells were infected with adenovirus to overexpress or knock down the expression of PGC-1α and SIRT3. Cell viability and apoptosis were analyzed by CCK-8 and flow cytometry, respectively. Hexokinase 2, pyruvate kinase activities, as well as NAD+/NADH ratio and ATP concentration, were assessed by commercial kits. Glucose consumption was measured using the glucose oxidase method and lactic acid concentration was detected by lactate dehydrogenase kit. Expression levels of PGC-1 and SIRT3 were much lower in breast cancer patients, compared with the normal controls. Overexpression of PGC-1α or SIRT3 both significantly promoted the apoptosis and inhibited the proliferation in MCF-7 and MDA-MB-231 cells. Additionally, PGC-1α or SIRT3 also induced the inhibition of glycolysis metabolism. Moreover, the expression of SIRT3 was positively regulated by PGC-1α. Silencing SIRT3 partly reversed the negative effects of PGC-1α on glycolytic metabolism. These findings demonstrated that PGC-1α/SIRT3 regulated cell proliferation and apoptosis of breast cancer through altering glycolysis, which may provide novel therapeutic strategies for breast cancer.
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http://dx.doi.org/10.4149/neo_2020_200530N584DOI Listing
March 2021

CYT387, a Novel JAK2 Inhibitor, Suppresses IL-13-Induced Epidermal Barrier Dysfunction Via miR-143 Targeting IL-13Rα1 and STAT3.

Biochem Genet 2021 Apr 15;59(2):531-546. Epub 2020 Nov 15.

School of Mechanical and Material Engineering, North China University of Technology, No. 5 Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China.

Atopic dermatitis (AD) is a chronic inflammatory skin disease influencing not only children but also adults. It is well-known that AD has a complex pathogenesis without effective therapy. Herein, we explored the function and mechanism of CYT387, a novel JAK2 inhibitor, on epidermal barrier damage. HaCaT cells exposed with high-concentration Ca (1.8 mM) for 14 days were recruited for the model of keratinocytes (KC). The cell model of skin barrier damage was induced by IL-13, and KC markers such as filaggrin (FLG), loricrin (LOR), and involucrin (IVL) were detected to judge the success of the model. In this study, we found that miR-143 was lowly expressed whereas IL-13Rα1 was highly expressed in blood cells of patients with AD, indicating their negative correlation. Moreover, IL-13 treatment down-regulated miR-143 and up-regulated activated JAK2 and STAT3 phosphorylation, which was reversed by CYT387 administration. The dual-luciferase reporter assay verified that miR-143 could directly bind to 3'-UTR of IL-13Rα1, as well as STAT3. Furthermore, the function of CYT387 in the skin barrier damage induced by IL-13 was abolished by miR-143 inhibitor. Thus, CYT387 might alleviate IL-13-induced epidermal barrier damage via targeting IL-13Rα1 and STAT3 by miR-143 to repress inflammation. These findings revealed that the protective effects and the underlying mechanisms of CYT387 in AD, which provided evidence that miR-143 may be a novel therapeutic target for AD.
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http://dx.doi.org/10.1007/s10528-020-10003-0DOI Listing
April 2021

Portable Nanohybrid Paper-Based Chemiresistive Sensor for Free Chlorine Detection.

ACS Omega 2020 Oct 21;5(39):25209-25215. Epub 2020 Sep 21.

Department of Mechanical Engineering, National Taipei University of Technology, Taipei 10608, Taiwan.

Detecting the concentration of free chlorine is important for monitoring the quality of water. In this study, we report a nanohybrid paper-based chemiresistive sensor that can be used with smartphones to detect free chlorine ions. The sensor was fabricated using a simple and standardized coating process. The graphene and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) nanohybrid paper-based sensing device exhibited a more stable and intuitive response to free chlorine than that exhibited by the device using only PEDOT:PSS. The nanohybrid paper-based sensor was sensitive to free chlorine concentrations in a linear range of 0.1-500 ppm, and the limit of detection was 0.18 ppm. The sensor showed specificity for free chloride ions and detection capability in samples. The sensor was integrated as a module with an electric readout system, and the measured signals and results could be displayed in real time on a smartphone. Therefore, the proposed sensing platform is suitable owing to its portability, low cost, ease of use, and capability for on-site water quality measurement.
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http://dx.doi.org/10.1021/acsomega.0c03366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542854PMC
October 2020

Red nucleus IL-6 mediates the maintenance of neuropathic pain by inducing the productions of TNF-α and IL-1β through the JAK2/STAT3 and ERK signaling pathways.

Neuropathology 2020 Aug 7;40(4):347-357. Epub 2020 May 7.

Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

We previously reported that interleukin (IL)-6 in the red nucleus (RN) is involved in the maintenance of neuropathic pain induced by spared nerve injury (SNI), and exerts a facilitatory effect via Janus-activated kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) and extracellular signal-regulated kinase (ERK) signal transduction pathways. The present study aimed at investigating the roles of tumor necrosis factor-α (TNF-α) and IL-1β in RN IL-6-mediated maintenance of neuropathic pain and related signal transduction pathways. Being similar to the elevation of RN IL-6 three weeks after SNI, increased protein levels of both TNF-α and IL-1β were also observed in the contralateral RN three weeks after the nerve injury. The upregulations of TNF-α and IL-1β were closely correlative with IL-6 and suppressed by intrarubral injection of a neutralizing antibody against IL-6. Administration of either the JAK2 antagonist AG490 or the ERK antagonist PD98059 to the RN of rats with SNI remarkably increased the paw withdrawal threshold (PWT) and inhibited the up-regulations of local TNF-α and IL-1β. Further experiments indicated that intrarubral injection of exogenous IL-6 in naive rats apparently lowered the PWT of the contralateral hindpaw and boosted the local expressions of TNF-α and IL-1β. Pretreatment with AG490 could block IL-6-induced tactile hypersensitivity and suppress the up-regulations of both TNF-α and IL-1β. However, injection of PD98059 in advance only inhibited the upregulation of IL-1β, but not TNF-α. These findings indicate that RN IL-6 mediates the maintenance of neuropathic pain by inducing the productions of TNF-α and IL-1β. IL-6 induces the expression of TNF-α through the JAK2/STAT3 pathway, and the production of IL-1β through the JAK2/STAT3 and ERK pathways.
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http://dx.doi.org/10.1111/neup.12653DOI Listing
August 2020

Nanofibrillar Poly(vinyl alcohol) Ionic Organohydrogels for Smart Contact Lens and Human-Interactive Sensing.

ACS Appl Mater Interfaces 2020 May 6;12(20):23514-23522. Epub 2020 May 6.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China.

Hydrogel bioelectronics as one of the next-generation wearable and implantable electronics ensures excellent biocompatibility and softness to link the human body and electronics. However, volatile, opaque, and fragile features of hydrogels due to the sparse and microscale three-dimensional network seriously limit their practical applications. Here, we report a type of smart and robust nanofibrillar poly(vinyl alcohol) (PVA) organohydrogels fabricated via one-step physical cross-linking. The nanofibrillar network cross-linked by numerous PVA nanocrystallites enables the formation of organohydrogels with high transparency (90%), drying resistance, high toughness (3.2 MJ/m), and tensile strength (1.4 MPa). For strain sensor application, the PVA ionic organohydrogel after soaking in NaCl solution shows excellent linear sensitivity (GF = 1.56, > 0.998) owing to the homogeneous nanofibrillar PVA network. We demonstrate the potential applications of the nanofibrillar PVA-based organohydrogel in smart contact lens and emotion recognition. Such a strategy paves an effective way to fabricate strong, tough, biocompatible, and ionically conductive organohydrogels, shedding light on multifunctional sensing applications in next-generation flexible bioelectronics.
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http://dx.doi.org/10.1021/acsami.0c06263DOI Listing
May 2020

Platelet indices significantly correlate with liver fibrosis in HCV-infected patients.

PLoS One 2020 9;15(1):e0227544. Epub 2020 Jan 9.

Dalian Blood Center, Zhongshan District, Dalian, Liaoning, China.

Aim: A total of 241 patients with chronic HCV infection were recruited to investigate the association between liver fibrosis and PLT counts, as well as with MPV, PDW and P-LCR indices.

Methods: The determination of PLT indices was carried out using a Sysmex XT-1800i automated hematology analyzer. Serological tests for HA, LN, C-IV and PIIINP were performed in 210 patients. The liver stiffness was measured in 69 patients by transient elastography (FibroScan).

Results: The analysis showed that the four serum fibrosis markers were negatively correlated with PLT counts, but positively correlated with the MPV, PDW and P-LCR values. Moreover, a similar pattern was found after analyzing the FibroScan measurements, which were negatively correlated with PLT counts, but positively correlated with MPV, PDW and P-LCR values. We subdivided the HCV-infected patients into mild and advanced fibrosis groups. The PLT counts were significantly decreased and the MPV, PDW and P-LCR values were significantly increased in the advanced fibrosis group when compared with the mild fibrosis group.

Conclusions: Our results demonstrate that not only the PLT counts but also the MPV, PDW and P-LCR indices significantly correlate with liver fibrosis in HCV-infected patients. Therefore, these indices may be useful laboratory measures for evaluating liver fibrosis progression.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227544PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952095PMC
April 2020

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis.

J Orthop Surg Res 2019 Sep 11;14(1):307. Epub 2019 Sep 11.

School of Mechanical and Material Engineering, North China University of Technology, No.5, Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China.

Background: Osteoarthritis (OA) is the common chronic degenerative joint bone disease that is mainly featured by joint stiffness and cartilage degradation. Icariin (ICA), an extract from Epimedium, has been preliminarily proven to show anti-osteoporotic and anti-inflammatory effects in OA. However, the underlying mechanisms of ICA on chondrocytes need to be elucidated.

Methods: LPS-treated chondrocytes and monosodium iodoacetate (MIA)-treated Wistar rats were used as models of OA in vitro and in vivo, respectively. LDH and MTT assays were performed to detect cytotoxicity and cell viability. The expression levels of NLRP3, IL-1β, IL-18, MMP-1, MMP-13, and collagen II were detected by qRT-PCR and Western blotting. The release levels of IL-1β and IL-18 were detected by ELISA assay. Caspase-1 activity was assessed by flow cytometry. Immunofluorescence and immunohistochemistry were used to examine the level of NLRP3 in chondrocytes and rat cartilage, respectively. The progression of OA was monitored with hematoxylin-eosin (H&E) staining and safranin O/fast green staining.

Results: ICA could suppress LPS-induced inflammation and reduction of collagen formation in chondrocytes. Furthermore, ICA could inhibit NLRP3 inflammasome-mediated caspase-1 signaling pathway to alleviate pyroptosis induced by LPS. Overexpression of NLRP3 reversed the above changes caused by ICA. It was further confirmed in the rat OA model that ICA alleviated OA by inhibiting NLRP3-mediated pyroptosis.

Conclusion: ICA inhibited OA via repressing NLRP3/caspase-1 signaling-mediated pyroptosis in models of OA in vitro and in vivo, suggesting that ICA might be a promising compound in the treatment of OA.
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http://dx.doi.org/10.1186/s13018-019-1307-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737611PMC
September 2019

Hydrogen Storage, Magnetism and Electrochromism of Silver Doped FAU Zeolite: First-Principles Calculations and Molecular Simulations.

Polymers (Basel) 2019 Feb 7;11(2). Epub 2019 Feb 7.

Heilongjiang Provincial Key Laboratory of Dielectric Engineering, School of Electrical and Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China.

The complex configuration, H₂ adsorption binding energy, magnetic, and optical properties of FAU zeolites with Ag cations loaded by ion exchange in the vacant dielectric cavities were investigated by employing the first-principles calculations with all-electron-relativistic numerical atom-orbitals scheme and the Metropolis Monte Carlo molecular simulations. The visible absorption spectrum peaked at distinct wavelengths arranging from red or green to blue colors when changing the net charge load, due to the produced various redox states of Ag cations exchanging at multiple Li⁺-substituted sites. The spin population analyses indicate the ferrimagnetic coupling between Al⁻O⁻Si framework and Ag cations originates from the major ferromagnetic spin polarization in Ag cation cluster coordinating with sodalite cages, with the net spins appreciably depending on the Ag content and exchange site. The H₂ adsorption capacities and binding energies represent significant dependence on the content, location, and electronic property of Ag cations introduced into the FAU zeolites. The evident decrease of H₂ adsorption binding energy with increased loading concentration demonstrates repulsive interaction between H₂ molecules and heterogeneous adsorption configuration on Ag cations. The adsorption sites of H₂ sorted by the binding energy with different adsorption configurations were correlated with exchange sites of Ag cations under different Ag loading to comprehend the H₂ adsorption mechanism.
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http://dx.doi.org/10.3390/polym11020279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419046PMC
February 2019

[Hypobaric Hypoxia Induces Autophagy of Umbilical Cord Mesenchymal Stem Cells].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Aug;26(4):1194-1197

Department of Hematology, Center of Hematologic Diseases of Chinese PLA, Lanzhou Military Command General Hospital, Lanzhou 730050, Gansu Province, China E-mail:

Objective: To investigate the autophagy activity changes of umbilical cord mesenchymal cells (MSC) under hypobaric hypoxia and the effect of hypobaric hypoxia on cell viability.

Methods: Umbilical cord mesenchymal cells were cultured in the chamber of hypobaric hypoxia with an air pressure of 41.1 kPa and an oxygen density of 1%. At 0, 4, 8, 16, 24 and 48 hours, the cells were harvested for Western blot and real-time PCR to observe the expression level of the autophagy marker protein LC3B. And the cell viability under hypobaric hypoxia was evaluated after treatment with autophagy inhibitors HCQ (8 μg/ml) and 3MA (5 mmol/L).

Results: LC3B expression in MSC at protein and mRNA levels were up-regulated significantly after being cultured under hypobaric hypoxia condition for 8 hours. And compared with the control group, inhibition of autophagy reduced cell viability while increased Caspase-3 expression and the incidence of apoptosis.

Conclusion: Hypobaric hypoxia activates autophagy in MSC, and the activation of autophagy might play a protective role for cell survival.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2018.04.041DOI Listing
August 2018

Sugar-sweetened beverage intake and serum testosterone levels in adult males 20-39 years old in the United States.

Reprod Biol Endocrinol 2018 Jun 23;16(1):61. Epub 2018 Jun 23.

The First Division in the Department of Endocrinology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 106th of Zhongshan Er Road, Guangzhou, 510080, China.

Background: This population-based study was designed to investigate whether consumption of sugar-sweetened beverages (SSB) is associated with lower serum total testosterone concentration in men 20-39 years old.

Methods: All data for this study were retrieved from the National Health and Nutrition Examination Survey (NHANES) 2011-2012. The primary outcome was serum testosterone concentration, and main independent variable was SSB intake. Other variables included age, race/ethnicity, poverty/income ratio, body mass index (BMI), serum cotinine, heavy drinking, and physical activity.

Results: Among all subjects (N = 545), 486 (90.4%) had normal testosterone levels (defined as ≥231 ng/dL) and 59 (9.6%) had low testosterone levels (defined as < 231 ng/dL). Multivariate logistic regression revealed the odds of low testosterone was significantly greater with increasing SSB consumption (Q4 [≥442 kcal/day] vs. Q1 [≤137 kcal/day]), adjusted odds ratio [aOR] = 2.29, p = 0.041]. After adjusting for possible confounding variables, BMI was an independent risk factor for low testosterone level; subjects with BMI ≥ 25 kg/m had a higher risk of having a low testosterone level than those with BMI < 25 kg/m (aOR = 3.68, p = 0.044).

Conclusion: SSB consumption is significantly associated with low serum testosterone in men 20-39 years old in the United States.
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http://dx.doi.org/10.1186/s12958-018-0378-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015465PMC
June 2018

[Assessment of hypoglycemic status among hospitalized elderly patients with type 2 diabetes].

Nan Fang Yi Ke Da Xue Xue Bao 2018 May;38(5):591-595

Southern Medical University, Guangzhou 510515, China.E-mail:

Objective: To investigate the hypoglycemic characteristics of hospitalized elderly patients with type 2 diabetes mellitus (T2DM).

Methods: From January, 2014 to December, 2015, the data of 58 565 blood measurements using a standard blood glucose monitoring system (BGMS) were collected from 1187 cases of patients with type 2 diabetes during hospitalization in the Department of Endocrinology, Guangdong General Hospital (Guangzhou, China). Stratified analyses were conducted by dividing the patients into 3 age groups, namely <45 years group (128 cases), 45-64 years group (594 cases), and ≥65 years group (465 cases). The incidence and time distribution of hypoglycemia in these patients were compared among the 3 age groups.

Results: The risk of hypoglycemia increased with age. Compared with those below 45 years of age, the patients beyond or equal to 65 years had a significantly increased hypoglycemic density (0.95% vs 0.40%, P<0.001), a higher proportion of patients with hypoglycemia (28.17% vs 10.94%, P<0.001), and greater patient-days with hypoglycemia (4.48% vs 1.76%, P<0.001). In the elderly patients, hypoglycemia occurred most frequently before dawn, at which time the hypoglycemic density was 2.66% in patients ≥65 years of age, significantly higher than that in patients below 45 years (1.09%, P<0.05) and between 45 and 64 years (1.90%, P<0.05); the proportion of patients with hypoglycemia was also significantly higher in the elderly patients (14.57%) than in those below 45 years (3.77%, P<0.02) and between 45 and 64 years (9.42%, P<0.02). The proportion of patients with recurrent hypoglycemia (≥2 times) was significantly higher in patients ≥65 years (13.33%) than in younger patients (2.34% in <45 years group and 9.43% in 45-64 years group, P<0.05).

Conclusion: The hypoglycemic risk in hospitalized elderly patients with T2DM is significantly higher than that in younger patients, especially before dawn and in terms of recurrent hypoglycemia. Clinicians should develop differential blood glucose monitoring and management strategies for these elderly patients to improve the clinical safety.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743887PMC
May 2018

Association between the polymorphism of HLA and ESRD in Dalian Han population located in north of China.

Immunol Invest 2018 Feb 19;47(2):212-219. Epub 2017 Dec 19.

a Blood Group Research Department, Dalian Blood Center , Dalian , Liaoning , China.

Background: End-stage renal disease (ESRD), the last stage of chronic renal failure, is a global health problem. The number of ESRD patients worldwide is increasing faster than the number of kidneys available per year for renal transplantation. Most of the ESRD patients are awaiting renal transplantation. The immune response to the transplanted kidney is directed mainly against mismatched human leukocyte antigen (HLA) glycoproteins expressed on donor tissues. Thus, the analysis of HLA allele and haplotype polymorphisms is valuable not only for identifying ESRD susceptibility factors but also to improve graft survival.

Methods: In this study, 163 Han ESRD patients were recruited to participate. The blood samples were genotyped by sequence-specific oligonucleotide method. A group of 14,529 healthy Chinese Han individuals registered at the Dalian Blood Center as bone marrow donors, living in the same region and of the same ethnicity, were used as controls.

Results: We found that only one allele, HLA-DRB1*12, showed a positive association with ESRD (p = 0.004, p = 0.028, odds ratio = 1.530, 95% confidence interval = 1.147-2.041); A*02-B*40-DRB1*09, A*02-B*40-DRB1*12, A*24-B*15-DRB1*12, and B*40-DRB1*12 were significantly more frequent in ESRD patients after Bonferroni correction (p < 0.05).

Conclusion: They were potentially valuable predictors for evaluating the risk of ESRD in the Dalian Han population.
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http://dx.doi.org/10.1080/08820139.2017.1416397DOI Listing
February 2018

Atypical Chemokine Receptor 1 polymorphism cannot be used as an indicator of liver fibrosis progression in Hepatitis C virus positive patients.

Pak J Med Sci 2017 Sep-Oct;33(5):1134-1137

Ming Liu, Department of Cell Biology, Dalian Medical University, Dalian, 116044, China.

Background & Objective: Atypical chemokine receptor 1(ACKR1) represents an atypical chemokine receptor that can bind promiscuously to various chemokines. Chemokines play a crucial role to recruit leukocyte subsets migration through the endothelium and into liver against the virus during the progression of hepatitis C virus (HCV) infection. Most HCV positive patients can lead to liver fibrosis. Hyaluronic acid (HA), laminin (LN), collagen IV(C-IV) and amino-terminal pro-peptide of Type-III pro-collagen (PIII NP) are indices of the extent of liver fibrosis. The aim of this study was to investigate the association between ACKR1 polymorphism and liver fibrosis with these four serum liver markers in HCV positive patients.

Methods: From April 2015 to December 2015, a total of 210 patients (109 males and 101 females) with chronic HCV infection at Dalian Infectious Hospital were recruited to participate in this study. ACKR1 genotyping was using TaqMan probes method. HA, LN, C-IV and PIII NP were detected by using diagnostic kits.

Results: We compared serum levels of HA, LN, C-IV and PIII NP between and patients and the differences were not significant (=0.905, =0.298, =0.880 and =0.470, respectively).

Conclusions: This study has attempted to elucidate the role of ACKR1 polymorphism in liver fibrosis progression of HCV infection, our results demonstrated that ACKR1 polymorphism is not directly associated with the fibrogenesis in HCV positive patients.
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http://dx.doi.org/10.12669/pjms.335.12590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673721PMC
November 2017

Association between Fibrinogen and Leukoaraiosis in Patients with Ischemic Stroke and Atrial Fibrillation.

J Stroke Cerebrovasc Dis 2017 Nov 16;26(11):2630-2637. Epub 2017 Aug 16.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China. Electronic address:

Background: Leukoaraiosis (LA), a surrogate of cerebral small-vessel diseases (CSVD), has been increasingly recognized because of its high prevalence and strong prognostic value in stroke. But the mechanism of LA is incompletely clarified. Fibrinogen is a crucial role in coagulation cascade and inflammation. There are inconsistent reports on the association of fibrinogen with LA in the general population. We aimed to investigate the association between fibrinogen and LA in patients with stroke and atrial fibrillation (AF), which was not ever reported before.

Methods: Patients with ischemic stroke and AF were prospectively and consecutively recruited. Clinico-demographic data and fibrinogen levels were collected within 48 hours from stroke onsets and analyzed according to the presence and distribution of LA (periventricular hyperintensity [PVH] and deep white matter hyperintensity).

Results: Of 186 patients (34.4% male; mean age, 68.76 ± 12.76 years) enrolled, 134 patients (72.0%) presented with LA. Elevated fibrinogen levels were associated with higher presence of LA (P = .005) and PVH (P = .002). After adjustment for the confounders, the fibrinogen levels were independently correlated with LA and PVH (all P <.05). Patients with elevated fibrinogen levels (≥3.5 g/L) were more likely to present with LA and PVH, with the odds ratios of 14.037 (95% confidence interval [CI] 2.588-76.131) and 12.567 (95% CI 2.572-61.395), respectively.

Conclusion: This study found that fibrinogen was independently and positively associated with LA and PVH in patients with stroke and AF. These results provide further evidence for the key role of fibrinogen in LA, even the total CSVD burden.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2017.06.027DOI Listing
November 2017

Association between leukoaraiosis and hemorrhagic transformation after cardioembolic stroke due to atrial fibrillation and/or rheumatic heart disease.

J Neurol Sci 2017 Jul 2;378:94-99. Epub 2017 May 2.

Department of Neurology, West China Hospital, Sichuan University, No. 37 Guo Xue Xiang, Chengdu 610041, Sichuan Province, PR China. Electronic address:

Cardioembolic stroke due to atrial fibrillation (AF) and/or rheumatic heart disease (RHD) often involves hemorrhagic transformation (HT), and we examined whether leukoaraiosis (LA) was associated with HT in these cases. We prospectively enrolled 251 patients who were admitted to two hospitals within one month of experiencing cardioembolic stroke due to AF/RHD. LA severity was assessed using three visual rating scales. HT was identified in 99 patients (39.4%) based on baseline computed tomography (CT) and post-admission magnetic resonance imaging or second CT. Univariate analysis identified risk of HT as higher in the presence of frontal LA based on the age-related white matter changes scale and in the presence of anterior LA based on the VSS scale. Multivariate analysis confirmed that moderate to severe LA was independently associated with higher HT risk. Of the various sites affected in LA, frontal LA correlated with highest risk of HT (OR 3.199, 95%CI 1.555-6.580). These results suggest that moderate to severe LA, especially at periventricular and anterior sites, is associated with HT after cardioembolic stroke due to AF/RHD. These findings suggest the need to take LA into account as a HT risk factor when considering the use of anticoagulation and thrombolysis in these patients.
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http://dx.doi.org/10.1016/j.jns.2017.05.001DOI Listing
July 2017

Atypical Chemokine Receptor 1 Polymorphism can not Affect Susceptibility to Hepatitis C Virus.

Balkan Med J 2017 Aug 6;34(4):308-312. Epub 2017 Apr 6.

Department of Cell Biology, Dalian Medical University, Liaoning, China.

Background: Hepatitis C virus has infected 130 to 150 million individuals globally. Atypical chemokine receptor 1 has become a focus of research because of its diverse roles in different diseases. However, little is known regarding the association of atypical chemokine receptor 1 polymorphism with susceptibility to hepatitis C virus.

Aims: To determine the association of an atypical chemokine receptor 1 polymorphism (rs12075) with hepatitis C virus susceptibility.

Study Design: Case-control study.

Methods: We collected blood samples from 231 patients infected with hepatitis C virus and 239 blood donors as control subjects. Genotyping of atypical chemokine receptor 1 was performed using a 5'-nuclease assay with TaqMan-minor groove binding probes. Comparisons between hepatitis C virus-infected patients and control subjects were assessed using Fisher's exact test.

Results: The genotype frequencies of FY*A/FY*A, FY*A/FY*B and FY*B/FY*B were 86.1%, 13.9% and 0% in the patient group, and 86.2%, 13.4% and 0.4% in the control group, respectively. The difference in atypical chemokine receptor 1 genotype frequencies between hepatitis C virus-infected patients and control group was not significant (p=1.00, OR=1.004, 95% CI=0.594-1.695). FY*A and FY*B allele frequencies were 93.1% and 6.9% in the patient group, and 92.9% and 7.1% in the control group, respectively. The difference in atypical chemokine receptor 1 allele frequencies between hepatitis C virus-infected patients and the control group was not significant (p=1.00, OR=0.972, 95% CI=0.589-1.603).

Conclusion: Our result indicates that atypical chemokine receptor 1 polymorphism (rs12075) does not affect susceptibility to hepatitis C virus.
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http://dx.doi.org/10.4274/balkanmedj.2016.0766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615962PMC
August 2017

[Research Progress of Novel Small Molecule Drugs in the Treatment of Chronic Lymphocytic Leukemia -Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2017 Feb;25(1):244-248

Hematology Center, Lanzhou Military Command General Hospital, Lanzhou 730050, Gansu Province, China. E-mial:

Chronic lymphocytic leukemia (CLL), the most frequent adult leukemia in Western population, is characterized by accumulation of mature-looking CD5/19/23 B cells in peripheral blood, bone marrow, and lymphatic organs. Over the last 20 years, there has been a dramatic change in therapy for CLL, the complete response rate increased from the initial <5% to the current 40%-50%, this remarkable improvement has been attributable to combination of chemoimmunotherapy agents that have contributed to the backbone of therapy for patients with CLL. Especially over the past 5 years, there has been an explosion of new active agents that provide a very effective solution for patients with recurrent/refractory disease as well as those who harbor poor cytogenetic abnormalities. This review focuses on some of the novel small molecule drugs that have either been approved or are at the forefront of clinical development in the treatment of patients with CLL, including tyrosine kinase inhibitior ibrutinib, PI3K inhibitor idelalisib, Syk inhibitor, BCL-2 inhibitor and so on.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2017.01.044DOI Listing
February 2017

Continuous positive airway pressure and diabetes risk in sleep apnea patients: A systemic review and meta-analysis.

Eur J Intern Med 2017 Apr 1;39:39-50. Epub 2016 Dec 1.

The First Division in the Department of Endocrinology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: The study assessed the effect of continuous positive airway pressure (CPAP) therapy on the risk of developing type 2 diabetes by evaluating change in the homeostasis model assessment of insulin resistance (HOMA-IR) fasting blood glucose (FBG) and fasting insulin following CPAP treatment in non-diabetic patients and pre-diabetic with obstructive sleep apnea (OSA).

Methods: Medline, PubMed, Cochrane, and EMBASE databases were searched until August 24, 2015. The analysis included randomized controlled trials (RCTs), two arm prospective studies, cohort studies, and retrospective studies. The primary outcome measure was change of HOMA-IR in pre-diabetic patients receiving CPAP treatment.

Results: Twenty-three studies were included with 965 patients who had OSA. Nineteen studies were prospective studies and four were RCTs. CPAP therapy resulted in a significant reduction in the pooled standard difference in means of HOMA-IR (-0.442, P=0.001) from baseline levels compared with the control group. Change in FBG and fasting insulin from baseline levels was similar for the CPAP and control groups. For RCT studies (n=4), there was no difference in change in HOMA-IR or FBG levels from baseline between CPAP and control groups. The combined effect of RCTs showed that CPAP was associated with a significant reduction in change from baseline in fasting insulin than the control group (standardized diff. in means between groups=-0.479, P value=0.003).

Conclusion: These findings support the use of CPAP in non-diabetic and pre-diabetic patients with OSA to reduce change of HOMA-IR and possibly reduce the risk of developing type 2 diabetes in this patient population.
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http://dx.doi.org/10.1016/j.ejim.2016.11.010DOI Listing
April 2017

Predictors of cardiorespiratory fitness in female and male adults with different body mass index: National Health and Nutrition Examination Survey 1999-2004 dataset.

Ann Med 2017 02 29;49(1):83-92. Epub 2016 Nov 29.

a The First Division in the Department of Endocrinology , Guangdong General Hospital, Guangdong Academy of Medical Sciences , Guangzhou , China.

Background: The aim of this study was to explore factors affecting cardiorespiratory fitness in males and females with different body mass index (BMI).

Methods: The National Health and Nutrition Examination Survey 1999-2004 data were used for this retrospective study. Estimated maximal oxygen uptake (VO) is surrogate for cardiorespiratory fitness (CRF). Univariate and multivariate linear regression analyses were performed to explore whether study variables were associated with estimated VO stratified by gender and BMI categories.

Results: A total of 3292 subjects 20-49 years of age were included in the analysis. CRF significantly decreased as BMI increased in both females and males. Ethnic difference was found in normal BMI in both genders and obese females; homocysteine was significantly negatively associated with estimated VO, as was total cholesterol. Obese male subjects with diabetes had a lower estimated VO than those without diabetes, and C-reactive protein (CRP) level and vitamin B12 level were significantly negatively associated with CRF. Female subjects with diabetes had higher estimated VO than those without diabetes. Folate was significantly positively correlated with estimated VO, whereas CRP was negatively correlated in obese female.

Conclusions: There are different predictors of CRF in males and females, and in individuals with different BMI. Key messages Different BMI classes are associated with different predictors of cardiorespiratory fitness. Indicators of cardiorespiratory fitness differ between sexes.
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http://dx.doi.org/10.1080/07853890.2016.1252056DOI Listing
February 2017

[Level and the Courses of Heavy Metals and Its Risk Assessment in Indoor Dust of City: Take Guiyang as a Case].

Huan Jing Ke Xue 2016 Aug;37(8):2889-2896

School of Geographic and Environmental Sciences, Guizhou Normal University, Guiyang 550001, China.

A total of 73 household dust and 6 office dust were collected and the concentrations of Ca, Fe, Cd, Cr, Cu, Ni, Pb and Zn were measured by ICP-OES, in order to study the levels of heavy metals in city indoor dust and assessits risk from indoor and outdoor dust to children. The result showed that: 1 The concentrations of Ca, Fe were 107, 31.9 g·kg and those of Cd, Cr, Cu, Ni, Pb and Zn were 1.77, 107, 231, 81.9, 199, 721 mg·kg, respectively. 2The levels of Cu and Zn in office dust were significantly higher than those in household dust, and the levels of other elements had no obvious difference from those in household dust. 3The levels of Ca and Fe in household dust with different floor numbers were not significantly different, but the levels of Cd, Cu and Pb in household dust with different floor numbers had obvious difference. The levels of elements in household dust from 1th floor were relatively higher, and the level of Pb in household dust from higher floors was higher than that on lower floors. 4Outdoor environment, indoor decoration and life styles may cause the difference of elements level in different household dust. 5There was no obvious risk from heavy metals in dust to children.
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http://dx.doi.org/10.13277/j.hjkx.2016.08.009DOI Listing
August 2016

[Seasonal Provincial Characteristics of Vertical Distribution of Dust Loadings and Heavy Metals near Surface in City].

Huan Jing Ke Xue 2015 Jun;36(6):2274-82

With the emergence of urban high-rise building, the vertical space of human daily life gradually extended upward. Seasonal characteristics of vertical distribution of dust loadings and heavy metals near surface are remarkable. In this study, we collected dust deposited on the windowsill at different space height (1th-8th floor) from three buildings in Guiyang city during spring, summer, autumn and winter, and analyzed the deposition fluxes of dust and elements including Ca, Fe, Cd, Cr, Cu, Ni, Pb and Zn. The results showed that: the total changing trend of vertical distribution of dust loadings was that the deposition fluxes of dust in winter were the highest, followed by those in spring, and the deposition fluxes of dust in summer were the lowest. The degree of variation on dust loadings dependent on the change of elevation was the highest in winter, followed by that in summer, and was relatively lower in spring and autumn. The effect on dust loadings by seasonal changing was relatively heavier on windowsill on the lower level than on the higher level. The levels of elements were the highest in spring dust, while those in autumn were relatively lower. Among the 8 elements, the variability of Zn in dust related to space time variation was the most obvious, and that of Ca was weaker. The atmospheric inversion condition might be one of the reasons that improved the deposition fluxes of dust and the contents of Ph and Zn in dust during winter and spring.
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June 2015

[Mechanism of biological actions of quercetin based on biomolecular network].

Yao Xue Xue Bao 2014 May;49(5):661-5

The mechanism of biological actions of quercetin was studied by using metabolomic method and biomolecular network. HPLC-MS was used to analyze the serum metabolome in rats of blank group and quercetin administration group rats, and MS data were processed by MATLAB software. With multivariate statistical analysis of serum metabolite profiles, a clear separation among blank group and quercetin administration group was achieved, potential biomarkers were selected according to the parameters of variable importance in the projection (VIP) and identified according to MS information and database retrieval. Four compounds, related enzymes, action targets and metabolic pathways had been confirmed, namely retinoic acid and RARbeta, arachidonate and COX-2, 3, 5-diodotyrosine and TPO, uridine diphosphate glucose and PDEs. The mechanism of quercetin enhancing ability of retinoic acid on the induction of RARbeta, activating TPO, using as COX-2 and PDEs inhibitor was approved by biomolecular network and related literatures. In this study, a mechanism of multiple biological actions of quercetin was evaluated at the level of the biomolecular network, metabolomics and biomolecular network can be used to investigate the biological effects mechanism of quercetin, which provided a new method to further revealing mechanism of drug action.
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May 2014

[Real-time PCR detection and quantification of emerging waterborne pathogens (EWPs) and antibiotic resistance genes (ARGs) in the downstream area of Jiulong River].

Huan Jing Ke Xue 2012 Aug;33(8):2685-90

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.

The emerging waterborne pathogens (EWPs) and antibiotic resistance genes (ARGs) are important for drinking water safety. The detection and quantification of 7 EWPs and 4 ARGs were carried out in Jiulong River, which is the main water source of southwestern Fujian Province. The water samples were collected from four sites of the Jiulong River downstream area and a drinking water treatment plant nearby. DNA was extracted and quantified by real-time (SYBR Green) PCR methods after the samples were filtered through 0.22 pim membranes. The results showed that the amount of Salmonella enterica (Salmonella spp.), Legionella pneumophila (L. pneumophila) and Pseudomonas aeruginosa (P. aeruginosa) could reach up to 10(3), 10(4) and 10(5) copies x mL(-1), respectively. The concentration of organic matter in water may affect the copy numbers significantly. The water plant could effectively remove most EWPs and ARGs except Salmonella spp.. Therefore, more efforts should be made on water pollution source control, water treatment technology and point-of-use system to make sure the safety of drinking water.
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August 2012

[Mechanism of NH(4+)-N removal in drinking water biofilter].

Huan Jing Ke Xue 2012 Jul;33(7):2394-402

School of Urban and Environmental Science, Xinyang Normal University, Xinyang 464000, China. Institute of Urban.

In order to explore the mechanism of NH(4+)-N removal in drinking water biofilter, water quality parameters, such as NH(4+)-N, NO(2-)-N, NO(3-)-N, total phosphorus, permanganate index, nitrogen gas, temperature and dissolved oxygen etc, were determined in the inflow and outflow of biofilter. Samples of granular activated carbon (GAC) at different height (0, 10, 20, 40, 60 cm) of the biofiter media were collected and analyzed for the bacterial community with molecular biology techniques. The bacterial diversity in the activated carbon biofilm sample was studied based on the phylogenetic analysis of sequences. The results showed that there were three stages according to the NH(4+)-N concentration in the influent. The "nitrogen loss" phenomenon (total inorganic nitrogen in the effluent was less than that in the influent) occurred at the first, second and third stages and the amount of nitrogen loss were 0.94, 0.32 and 0.15 mg x L(-1), respectively. The amount of nitrogen loss had a good positive correlation with the NH(4+)-N concentration in the influent, but not a linear relationship with the concentration of the permanganate index in the influent. The average concentrations of N2 increased gradually with the height of media in the biofilter, with values of 14.04 and 14.67 mg x L(-1) in the influent and the effluent, respectively. Based on the sequencing results, the ammonia-oxidizing bacteria (AOB) in the activated carbon biofilm were classified into three common genera: Nitrosococcus, Nitrosomonas and Nitrosospira. When the NH(4+)-N concentration in the influent was relatively high, the "nitrogen loss" phenomenon in biofilter was caused by the AOB.
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July 2012

[Clinical characteristics and long-term prognosis of patients with ischemic and hemorrhagic stroke].

Zhonghua Yi Xue Za Zhi 2008 Apr;88(13):892-7

Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To investigate the risk factors, pathogenesis, cause of death, and outcome of different stroke subtypes.

Methods: The relevant data, including demographics, baseline risk factors, cause of death, and 1-year case fatality, were analyzed among 1913 consecutive hospitalized patients with ischemic and hemorrhagic stroke, 599 (31.3%) with intracerebral hemorrhage (ICH) and 1314 with ischemic stroke (68.7%), including 209 cases (15.9%) of total anterior circulation infarction (TACI), 417 cases (31.7%) of partial anterior circulation infarction (PACI), and 186 cases (14.2%) of posterior circulation infarctions (POCI), and 502 cases (38.2%) of lacunar infarctions (LACI), 1098 males and 815 females, aged 64 +/- 13 (14-98).

Results: Multivariate analysis showed that when age and sex were adjusted, atrial fibrillation was the independent predictive factor of TACI [odds ratio (OR) = 1.42, 95% CI = 1.25-2.31), hypertension and alcohol intake were the independent predictive factor LACI (OR = 1.24, 95% CI = 1.02-2.18; 0R = 1.12, 95% CI = 1.03-3.04) and ICH (OR = 1.84, 95% CI = 1.31-3.02; OR = 1.04, 95% CI = 1.01-4.13). A negative association was observed between hypertension and TACI (OR = 0.62, 95% CI = 0.34-0.72), atrial fibrillation and LACI (OR = 0.46, 95% CI = 0.26-0.82), and ICH and diabetes (OR = 0.56, 95% CI = 0.42-0.76). As compared to LACI, TACI and ICH significantly increased the risk of 1-year mortality (OR = 6.21, 95% CI = 2.86-8.42; OR = 5.86; 95% CI = 2.46-8.52).

Conclusions: Stroke subtypes have different risk factor profile, causes and outcome. Information on determinants of the clinical syndromes may impact on the prevention and acute phase interventions.
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April 2008
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