Publications by authors named "Shu Liu"

690 Publications

[Retracted] miR‑124 inhibits proliferation and invasion of human retinoblastoma cells by targeting STAT3.

Oncol Rep 2021 Nov 24;46(5). Epub 2021 Sep 24.

Department of Ophthalmology, The Second Hospital of Jilin University, Nanguan, Changchun, Jilin 130041, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that the GAPDH bands shown in the western blots in Fig. 6A were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they agreed with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 36: 2398‑2404, 2016; DOI: 10.3892/or.2016.4999].
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http://dx.doi.org/10.3892/or.2021.8193DOI Listing
November 2021

Inflammation Disturbed the Tryptophan Catabolites in Hippocampus of Post-operative Fatigue Syndrome Rats Indoleamine 2,3-Dioxygenas Enzyme and the Improvement Effect of Ginsenoside Rb1.

Front Neurosci 2021 26;15:652817. Epub 2021 Aug 26.

Department of Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai, China.

Aim: Post-operative fatigue syndrome (POFS) is a common complication that prolongs the recovery to normal function and activity after surgery. The aim of the present study was to explore the mechanism of central fatigue in POFS and the anti-fatigue effect of ginsenoside Rb1.

Method: We investigated the association between inflammation, indoleamine 2,3-dioxygenase (IDO) enzyme, and tryptophan metabolism in the hippocampus of POFS rats. A POFS rat model was induced by major small intestinal resection. Rats with major small intestinal resection were administered ginsenoside Rb1 (15 mg/kg) once a day from 3 days before surgery to the day of sacrifice, or with saline as corresponding controls. Fatigue was assessed with the open field test (OFT) and sucrose preference test (SPT). ELISA, RT-PCR, Western blot, immunofluorescence, and high-performance liquid chromatography (HPLC) were used to test the inflammatory cytokines; p38MAPK, NF-κB/p65, and IDO enzyme expressions; and the concentrations of tryptophan, kynurenine, and serotonin, respectively.

Result: Our results showed that POFS was associated with increased expressions of inflammatory cytokines and p38MAPK and higher concentrations of kynurenine and tryptophan on post-operative days 1 and 3; a lower serotonin level on post-operative day 1; and an enhanced translocation of NF-κB/p65 and the IDO enzyme on post-operative days 1, 3, and 5. Ginsenoside Rb1 had an improvement effect on these.

Conclusion: Inflammatory cytokines induced by large abdominal surgery disturb tryptophan metabolism to cause POFS through the activation of the p38MAPK-NF-κB/p65-IDO pathway in the hippocampus. Ginsenoside Rb1 had an anti-fatigue effect on POFS by reducing inflammation and IDO enzyme.
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http://dx.doi.org/10.3389/fnins.2021.652817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427665PMC
August 2021

Transmission of a Novel Imprinting Center Deletion Associated With Prader-Willi Syndrome Through Three Generations of a Chinese Family: Case Presentation, Differential Diagnosis, and a Lesson Worth Thinking About.

Front Genet 2021 24;12:630650. Epub 2021 Aug 24.

Obstetrics and Gynecology Hospital of Fudan University, Fudan University, Shanghai, China.

Prader-Willi syndrome (PWS) is a complex genetic syndrome caused by the loss of function of genes in 15q11-q13 that are subject to regulation by genomic imprinting and expressed from the paternal allele only. The main clinical features of PWS patients are hypotonia during the neonatal and infantile stages, accompanied by delayed neuropsychomotor development, hyperphagia, obesity, hypogonadism, short stature, small hands and feet, mental disabilities, and behavioral problems. However, PWS has a clinical overlap with other disorders, especially those with other gene variations or chromosomal imbalances but sharing part of the similar clinical manifestations with PWS, which are sometimes referred to as Prader-Willi syndrome-like (PWS-like) disorders. Furthermore, it is worth mentioning that significant obesity as a consequence of hyperphagia in PWS usually develops between the ages of 1 and 6 years, which makes early diagnosis difficult. Thus, PWS is often not clinically recognized in infants and, on the other hand, may be wrongly suspected in obese and intellectually disabled patients. Therefore, an accurate investigation is necessary to differentiate classical PWS from PWS-like phenotypes, which is imperative for further treatment. For PWS, it is usually sporadic, and very rare family history and affected siblings have been described. Here, we report the clinical and molecular findings in a three-generation family with a novel 550-kb microdeletion affecting the chromosome 15 imprinting center (IC). Overall, the present study finds that the symptoms of our patient are somewhat different from those of typical PWS cases diagnosed and given treatment in our hospital. The familial occurrence and clinical features were challenging to our diagnostic strategy. The microdeletion included a region within the complex small nuclear ribonucleoprotein polypeptide protein N () gene locus encompassing the PWS IC and was identified by using a variety of techniques. Haplotype studies suggest that the IC microdeletion was vertically transmitted from an unaffected paternal grandmother to an unaffected father and then caused PWS in two sibling grandchildren when the IC microdeletion was inherited paternally. Based on the results of our study, preimplantation genetic diagnosis (PGD) was applied successfully to exclude imprinting deficiency in preimplantation embryos before transfer into the mother's uterus. Our study may be especially instructive regarding accurate diagnosis, differential diagnosis, genetic counseling, and PGD for familial PWS patients.
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http://dx.doi.org/10.3389/fgene.2021.630650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421676PMC
August 2021

Extracellular Vesicle Separation Techniques Impact Results from Human Blood Samples: Considerations for Diagnostic Applications.

Int J Mol Sci 2021 Aug 26;22(17). Epub 2021 Aug 26.

Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, 53127 Bonn, Germany.

Extracellular vesicles (EVs) are reminiscent of their cell of origin and thus represent a valuable source of biomarkers. However, for EVs to be used as biomarkers in clinical practice, simple, comparable, and reproducible analytical methods must be applied. Although progress is being made in EV separation methods for human biofluids, the implementation of EV assays for clinical diagnosis and common guidelines are still lacking. We conducted a comprehensive analysis of established EV separation techniques from human serum and plasma, including ultracentrifugation and size exclusion chromatography (SEC), followed by concentration using (a) ultracentrifugation, (b) ultrafiltration, or (c) precipitation, and immunoaffinity isolation. We analyzed the size, number, protein, and miRNA content of the obtained EVs and assessed the functional delivery of EV cargo. Our results demonstrate that all methods led to an adequate yield of small EVs. While no significant difference in miRNA content was observed for the different separation methods, ultracentrifugation was best for subsequent flow cytometry analysis. Immunoaffinity isolation is not suitable for subsequent protein analyses. SEC + ultracentrifugation showed the best functional delivery of EV cargo. In summary, combining SEC with ultracentrifugation gives the highest yield of pure and functional EVs and allows reliable analysis of both protein and miRNA contents. We propose this combination as the preferred EV isolation method for biomarker studies from human serum or plasma.
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http://dx.doi.org/10.3390/ijms22179211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431127PMC
August 2021

[Potential targets of Euodiae Fructus in treatment of insomnia based on network pharmacology].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(12):3016-3023

the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China.

The acupoint application of Euodiae Fructus at Yongquan(KI1) can significantly improve the sleep quality of patients with insomnia with berberine as the main effective component for the efficacy. Nineteen active compounds and 203 drug targets were screened out from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). After comparison with GeneCards and Online Mendelian Inheritance in Man(OMIM), 24 common genes of diseases and drugs were obtained. STRING 11.0 was used to construct a protein-protein interaction(PPI) network of the overlapping genes, and Matthews correlation coefficient(MCC) was employed to screen the core genes, which were then subjected to enrichment analysis with gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG). The results revealed that the main compounds of Euodiae Fructus, such as berberine and rutaecarpine, participated in the biological processes(such as neurotransmitter receptor activity) by regulating C-reactive protein(CRP), estrogen receptor 1(ESR1), 5-hydroxytryptamine(5-HT) receptor, and interleukin-6(IL-6) to exert sedative, anxiolytic, and antidepressant effects. Sixty 4-week-old SPF mice were randomly divided into a control group, a model group, a positive drug(diazepam tablets) group, and low-, medium-, and high-dose berberine groups. Medication with corresponding drugs was performed for one week. The results demonstrated that berberine was potent in reducing the activities and standing times of mice, down-regulating the levels of CRP and IL-6 mRNA in the hypothalamus, and up-regulating the expression of 5-HT(P<0.01); however, no significant effect on ESR1 was observed. The network of Euodiae Fructus in treating insomnia was constructed by network pharmacology and verified by tests. The findings indicated that the therapeutic efficacy of Euodiae Fructus in treating insomnia was achieved by participating in multiple biological processes, such as neurotransmitter receptor activity, which provided a scientific basis for its clinical application.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210419.401DOI Listing
June 2021

Comprehensive physiopathology and serum metabolomics for the evaluation of the influence mechanism of qi deficiency on xenograft mouse models of liver cancer.

J Sep Sci 2021 Aug 18. Epub 2021 Aug 18.

Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.

Traditional Chinese medicine believes that qi deficiency is important pathogenesis and syndrome of liver cancer and thus is crucial in related research. However, the effect of qi deficiency on the occurrence and development of liver cancer is still unclear. This study aimed to establish a liver cancer model of qi deficiency through the swimming exhaustion and xenograft of human hepatoma HepG2 cells. The effects of qi deficiency on the occurrence and development of liver cancer were investigated by analyzing tumor development, blood routine, histopathology, and serum metabolomics. Results showed that qi deficiency greatly affected the physiology and tumor growth of xenograft mice. Eight potential biomarkers were identified by metabolomics based on ultra-high performance liquid chromatography and tandem quadrupole time-of-flight mass spectrometry. Their main pathways were arachidonic acid metabolism, phenylalanine metabolism, purine metabolism, glycerolipid metabolism, steroid biosynthesis, sphingomyelin metabolism, and fatty acid metabolism pathway. Finally, the effects of qi deficiency on the occurrence and development of liver cancer were comprehensively analyzed, and the mechanism of this process was preliminarily clarified.
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http://dx.doi.org/10.1002/jssc.202100260DOI Listing
August 2021

Quantum Wave Packet Study of the H + Br → HBr + Br Reaction on a New Ab Initio Potential Energy Surface.

J Phys Chem A 2021 Aug 12;125(33):7289-7296. Epub 2021 Aug 12.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China 116023.

An accurate global potential energy surface (PES) for the HBr system has been constructed using the fundamental invariant neural network fitting method based upon 11 698 energies at the UCCSD(T)/CBS level of theory, with the spin-orbit coupling of the P orbit of the Br atom properly included. The time-dependent wave packet calculations have been performed to study the H + Br → HBr + Br reaction on the new PES. The total reaction probabilities for total angular momentum = 0 for the ground initial state show no threshold due to the submerged barrier height (-0.351 kcal/mol) of the PES. The total integral cross sections (ICS) for reactant Br in ro-vibrational states ( = 0, = 0, 10, 20, 30; = 1-5, = 0) were calculated for collision energy of up to 0.5 eV. It is found that the initial rotational excitation has a negligible effect on the ICS, and the initial vibrational excitation depresses the reactivity to some extent. The thermal rate constants for the title reaction in the temperature range of 100-1000 K were calculated from the Boltzmann averaging of the = 0-5 rate constants, which overestimated the experimental results to some extent.
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http://dx.doi.org/10.1021/acs.jpca.1c05867DOI Listing
August 2021

NAP1L1 Functions as a Tumor Promoter Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.

Front Cell Dev Biol 2021 23;9:659680. Epub 2021 Jul 23.

Department of Clinical Medicine, Guizhou Medical University, Guiyang, China.

NAP1L1 has been reported to be significantly involved in the carcinogenesis of hepatocellular carcinoma (HCC). Yet, its detailed molecular basis is still to be determined. Based on the analysis of The Cancer Genome Atlas (TCGA) database, NAP1L1 mRNA was found to be upregulated and predicted the poor prognosis initially. Subsequently, consistent with the prediction, the upregulated expression of NAP1L1 mRNA and protein levels was confirmed by quantitative polymerase chain reaction (qPCR), Western blot, and immunohistochemistry assays. Upregulated NAP1L1 protein positively promoted the disease progression and poor prognosis of HCC. In addition, NAP1L1 protein expression was considered as an independent prognostic factor in HCC. Inhibition of NAP1L1 expression by siRNA or shRNA pathway significantly reduced the cell proliferation and cell cycle transformation and . Mechanism analysis first showed that the function of NAP1L1 was to recruit hepatoma-derived growth factor (HDGF), an oncogene candidate widely documented in tumors. Furthermore, the latter interacted with c-Jun, a key oncogenic transcription factor that can induce the expression of cell cycle factors and thus stimulate the cell growth in HCC. Finally, transfecting HDGF or c-Jun could reverse the suppressive effects on HCC growth in NAP1L1-suppressed HCC cells. Our data indicate that NAP1L1 is a potential oncogene and acts recruiting HDGF/c-Jun in HCC.
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http://dx.doi.org/10.3389/fcell.2021.659680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343235PMC
July 2021

Maternal PM Exposure Increases Risk for Spina Bifida: A Population-Based Case-Control Study.

Front Public Health 2021 21;9:695192. Epub 2021 Jul 21.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Limited studies have focused on the impact of ambient air pollution on spina bifida. A population-based case-control study was conducted in Liaoning Province, China to assess the associations between maternal PM exposures in various exposure windows and spina bifida risk. Data on spina bifida cases born between 2010 and 2015 were available from the Maternal and Child Health Certificate Registry of Liaoning Province. Controls were a random sample of healthy livebirths without any birth defects delivered in the selected five cities during 2010-2015. Ambient air monitoring data for PM were obtained from 75 monitoring stations in Liaoning Province. The multivariable logistic regression models were established to calculate adjusted odds ratios (OR) and 95% confidence intervals (CI). We further performed sensitivity analyses by using three propensity score methods. A total of 749 spina bifida cases and 7,950 controls were included. After adjusting for potential confounders, spina bifida was associated with a 10 μg/m increment in PM during the first trimester of pregnancy (adjusted OR = 1.06, 95% CI: 1.00-1.12) and the 3 months before pregnancy (adjusted OR = 1.12, 95% CI: 1.06-1.19). The adjusted ORs in the final model for the highest vs. the lowest quartile were 1.51 (95% CI: 1.04-2.19) for PM during the first trimester of pregnancy and 2.01 (95% CI: 1.43-2.81) for PM during the 3 months before pregnancy. Positive associations were found between PM exposures during the single month exposure windows and spina bifida. Sensitivity analyses based on two propensity score methods largely reported similar positive associations. Our findings support the evidence that maternal PM exposure increases the risk of spina bifida in offspring. Further, validation with a prospective design and a more accurate exposure assessment is warranted.
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http://dx.doi.org/10.3389/fpubh.2021.695192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333283PMC
August 2021

Female Labor Force Status and Couple's Marital Satisfaction: A Chinese Analysis.

Authors:
Xiao Yu Shu Liu

Front Psychol 2021 23;12:691460. Epub 2021 Jul 23.

Northeast Asian Research Center, Jilin University, Changchun, China.

While China has decreasing female labor participation and increasing marital instability, compared to the rest of the world, its female labor participation rate is higher on average. The effect of female labor force status on couples' marital satisfaction, as one of the main factors for evaluating marital quality, has been separately discussed, including extensive margins considering whether women are in the labor market and intensive margins on women working hours per week. This study analyzed data from the 2014 China Family Panel Studies (CFPS) using a binary logit model and a stability test. Results showed that the work hours, rather than the occupational status, of women affect marital satisfaction. In addition, regardless of the gender role attitudes held by the couple, marital satisfaction increases when women are in the labor market. This study has retroactively reviewed the effects of women working outside the home on marital quality. The dual roles of Chinese women, as both employee and homemaker, have been socially accepted. However, the requirements of maintaining multiple roles often contradict and present conflicts among the roles, time, and pressure, in the long run, giving rise to marital dissatisfaction.
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http://dx.doi.org/10.3389/fpsyg.2021.691460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343394PMC
July 2021

Based on urine metabolomics to study the mechanism of Qi-deficiency affecting type 2 diabetes rats using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Aug 7;1179:122850. Epub 2021 Jul 7.

School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. Electronic address:

Qi-deficiency also called energy deficiency, which approximates to the term of sub-health in contemporary medical theory. Diabetes is similar to the symptoms of "xiaoke" in traditional Chinese medicine (TCM) which is linked with Qi-deficiency. However, the mechanism of Qi-deficiency on type 2 diabetes (T2D) has not been completely elucidated. In this study, a model on Qi-deficiency T2D rat was established by using diet with high fat and high sugar and small-dose STZ induction combined with exhaustive swimming, and the model was evaluated by pathological section, hematological index and serum biochemical parameters. Applying urine metabolomics based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to explore the underlying molecular mechanism of Qi-deficiency on T2D and 32 urinary metabolites were identified as prospective biomarkers for Qi-deficiency T2D rats. Metabolic pathway analysis indicated that synthesis and degradation of ketone bodies, starch and sucrose metabolism, phenylalanine metabolism, arachidonic acid metabolism, butanoate metabolism and TCA cycle, etc., were closely related to potential mechanisms of Qi-deficiency on T2D. The metabolomics results can provide reliable data support for complex TCM syndrome diagnosis.
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http://dx.doi.org/10.1016/j.jchromb.2021.122850DOI Listing
August 2021

Hepatic cell mobilization for protection against ischemic myocardial injury.

Sci Rep 2021 Aug 4;11(1):15830. Epub 2021 Aug 4.

Biomedical Engineering Department, Northwestern University, Evanston, IL, 60208, USA.

The heart is capable of activating protective mechanisms in response to ischemic injury to support myocardial survival and performance. These mechanisms have been recognized primarily in the ischemic heart, involving paracrine signaling processes. Here, we report a distant cardioprotective mechanism involving hepatic cell mobilization to the ischemic myocardium in response to experimental myocardial ischemia-reperfusion (MI-R) injury. A parabiotic mouse model was generated by surgical skin-union of two mice and used to induce bilateral MI-R injury with unilateral hepatectomy, establishing concurrent gain- and loss-of-hepatic cell mobilization conditions. Hepatic cells, identified based on the cell-specific expression of enhanced YFP, were found in the ischemic myocardium of parabiotic mice with intact liver (0.2 ± 0.1%, 1.1 ± 0.3%, 2.7 ± 0.6, and 0.7 ± 0.4% at 1, 3, 5, and 10 days, respectively, in reference to the total cell nuclei), but not significantly in the ischemic myocardium of parabiotic mice with hepatectomy (0 ± 0%, 0.1 ± 0.1%, 0.3 ± 0.2%, and 0.08 ± 0.08% at the same time points). The mobilized hepatic cells were able to express and release trefoil factor 3 (TFF3), a protein mitigating MI-R injury as demonstrated in TFF3 mice (myocardium infarcts 17.6 ± 2.3%, 20.7 ± 2.6%, and 15.3 ± 3.8% at 1, 5, and 10 days, respectively) in reference to wildtype mice (11.7 ± 1.9%, 13.8 ± 2.3%, and 11.0 ± 1.8% at the same time points). These observations suggest that MI-R injury can induce hepatic cell mobilization to support myocardial survival by releasing TFF3.
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http://dx.doi.org/10.1038/s41598-021-94170-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339068PMC
August 2021

Percutaneous Transthoracic Lung Biopsy: Optimizing Yield and Mitigating Risk.

J Comput Assist Tomogr 2021 Sep-Oct 01;45(5):765-775

Abstract: Percutaneous computed tomography-guided transthoracic lung biopsy is an effective and minimally invasive procedure to achieve tissue diagnosis. Radiologists are key in appropriate referral for further workup, with percutaneous computed tomography-guided transthoracic lung biopsy performed by both thoracic and general interventionalists. Percutaneous computed tomography-guided transthoracic lung biopsy is increasingly performed for both diagnostic and research purposes, including molecular analysis. Multiple patient, lesion, and technique-related variables influence diagnostic accuracy and complication rates. A comprehensive understanding of these factors aids in procedure planning and may serve to maximize diagnostic yield while minimizing complications, even in the most challenging scenarios.
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http://dx.doi.org/10.1097/RCT.0000000000001192DOI Listing
August 2021

Prediction of Patient Height and Weight With a 3-Dimensional Camera.

J Comput Assist Tomogr 2021 May-Jun 01;45(3):427-430

From the Department of Radiology, NYU Langone Health, New York, NY.

Objective: The aim of this study was to determine accuracy of height and weight prediction by a 3-dimensional (3D) camera.

Methods: A total of 453 patients whose computed tomography imaging used a 3D camera from December 19, 2018 to March 19, 2019 were retrospectively identified. An image of each patient was taken before the computed tomography by a 3D camera mounted to the ceiling. Using infrared imaging and machine learning algorithms, patient height and weight were estimated from this 3D camera image. A total of 363 images were used for training. The test set consisted of 90 images. The height and weight estimates were compared with true height and weight to determine absolute and percent error. A value of P < 0.05 indicated statistical significance.

Results: There was 2.0% (SD, 1.4) error in height estimation by the 3D camera, corresponding to 3.35 cm (SD, 2.39) absolute deviation (P = 1, n = 86). Weight estimation error was 5.1% (SD, 4.3), corresponding to 3.99 kg (SD, 3.11) absolute error (P = 0.74, n = 90).

Conclusion: Pictures obtained from a 3D camera can accurately predict patient height and weight.
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http://dx.doi.org/10.1097/RCT.0000000000001166DOI Listing
July 2021

Maternal exposure to sulfur dioxide before and after conception and the risk of hypospadias in offspring.

Ann N Y Acad Sci 2021 Jul 11. Epub 2021 Jul 11.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.

As far as we know, there have been no studies exploring the association between maternal sulfur dioxide (SO ) exposure and the risk of hypospadias in offspring. We aimed to evaluate this association during the 3 months before conception and the first trimester. A population-based case-control study was conducted in male infants, consisting of 348 cases of hypospadias and 4023 controls. Maternal exposure to SO , particulate matter with aerodynamic diameters ≤10 μm (PM ), and nitrogen dioxide (NO ) was assessed by averaging the concentrations recorded at all stations in the mother's city of residence. Air pollutants were tested for multicollinearity using variance inflation factor analysis. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by multivariable logistic regression models. SO exposure during the 3 months before conception was significantly associated with the risk of hypospadias (highest tertile: OR = 7.40, 95% CI: 3.54-15.62). When focusing on shorter exposure windows, similar associations were observed for SO exposure in the first and second month before and the first month after conception. In conclusion, maternal exposure to SO during the 3 months before and the first and second months after conception may increase the risk of hypospadias in offspring.
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http://dx.doi.org/10.1111/nyas.14624DOI Listing
July 2021

Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils.

Nat Commun 2021 07 9;12(1):4231. Epub 2021 Jul 9.

German Center for Neurodegenerative Diseases (DZNE), Göttingen, Germany.

Pathological aggregation of the protein tau into insoluble aggregates is a hallmark of neurodegenerative diseases. The emergence of disease-specific tau aggregate structures termed tau strains, however, remains elusive. Here we show that full-length tau protein can be aggregated in the absence of co-factors into seeding-competent amyloid fibrils that sequester RNA. Using a combination of solid-state NMR spectroscopy and biochemical experiments we demonstrate that the co-factor-free amyloid fibrils of tau have a rigid core that is similar in size and location to the rigid core of tau fibrils purified from the brain of patients with corticobasal degeneration. In addition, we demonstrate that the N-terminal 30 residues of tau are immobilized during fibril formation, in agreement with the presence of an N-terminal epitope that is specifically detected by antibodies in pathological tau. Experiments in vitro and in biosensor cells further established that co-factor-free tau fibrils efficiently seed tau aggregation, while binding studies with different RNAs show that the co-factor-free tau fibrils strongly sequester RNA. Taken together the study provides a critical advance to reveal the molecular factors that guide aggregation towards disease-specific tau strains.
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http://dx.doi.org/10.1038/s41467-021-24362-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270918PMC
July 2021

Correction to: Identification of iron ore brands by multi-component analysis and chemometric tools.

Anal Bioanal Chem 2021 Aug 6;413(20):5253-5255. Epub 2021 Jul 6.

Technical Center for Industrial Product and Raw Material Inspection and Testing of Shanghai Customs, Shanghai, 200135, China.

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http://dx.doi.org/10.1007/s00216-021-03484-4DOI Listing
August 2021

Composting-a solution of eliminating a nitrite-rich wastewater by reusing it as a moisture conditioning agent.

Chemosphere 2021 Jun 28;284:131365. Epub 2021 Jun 28.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100089, China.

Composting could be applied to dispose various organic solid wastes and liquid wastes. Literature suggested that reusing a nitrogen-rich wastewater as a composting moisture conditioning agent could promote the maturity and nitrogen content of compost. However, it's unclear whether a nitrite-rich wastewater could be eliminated by composting because of the toxicity of nitrite. In this study, a nitrite-rich wastewater (STL, pH = 7.9) was reused as a composting moisture conditioning agent. The influence of STL reusing period (i.e., adding STL from the first day of mesophilic, thermophilic, and cooling period, and the addition lasted for 10 days) on composting performance was also discussed. Results revealed that organic matter decomposition was strongly suppressed by high concentration of free nitrous acid when STL was added in mesophilic period, whereas the organic matter hydrolysis was prompted when STL was added in thermophilic and cooling period. STL addition enhanced nitrification at high temperatures during composting, thus increasing the nitrate content of compost by 2-10 times compared with that of the control group (using tap water as a moisture conditioning agent). Nitrite addition also stimulated nitrous oxide emissions yielded by biotic or chemical processes during STL addition, especially under the transient condition at 50°C-55 °C, and resulted in a 28%-39% increase in greenhouse gas emissions compared with that of the control group. Therefore, the composting could be a solution of eliminating a nitrite-rich wastewater by reusing it as a moisture conditioning agent when nitrous oxide emission issue was properly addressed.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131365DOI Listing
June 2021

[Corrigendum] miR‑29a inhibits human retinoblastoma progression by targeting STAT3.

Oncol Rep 2021 Aug 29;46(2). Epub 2021 Jun 29.

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.

Following the publication of this article, an interested reader drew to the authors' attention that some western blotting data bands had apparently been duplicated in Fig. 4D. The authors have re‑examined their original data, and realized that this figure was assembled incorrectly. The corrected version of Fig. 4 is shown below. The authors sincerely apologize for the errors that were introduced during the preparation of this figure, and thank the Editor for allowing them the opportunity to publish a Corrigendum. Furthermore, they regret any inconvenience caused to the readership. [the original article was published in Oncology Reports 39: 739-746, 2018; DOI: 10.3892/or.2017.6144].
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http://dx.doi.org/10.3892/or.2021.8126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261205PMC
August 2021

Feshbach Resonances in the Vibrationally Excited F + HOD(/ = 1) Reaction Due to Chemical Bond Softening.

J Phys Chem Lett 2021 Jul 25;12(26):6090-6094. Epub 2021 Jun 25.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Experiments and theories showed the ground-state reaction F + HO → HF + OH possesses Feshbach resonances trapped in the hydrogen bond well in the product region. However, it is not clear whether F + HO and its isotopic analogues have the same Feshbach resonances caused by chemical bond softening as those in the F + H/HD. Here, we reported state-to-state quantum dynamics studies of the F + HOD( = 1) → HF + OD and F + HOD( = 1) → DF + OH reactions on an accurate neural network potential energy surface. Detailed analysis reveals that the course of the title reactions is dominated by the Feshbach resonance states trapped in the peculiar HF('=3)-OD/DF('=4)-OH vibrationally adiabatic potential well created by the HF/DF bond softening, which can only be accessed via the HOD( = 1)/HOD( = 1) reaction pathway. Therefore, we confirm the wide existence of chemical bond softening resonances in reactions involving vibrationally excited molecules.
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http://dx.doi.org/10.1021/acs.jpclett.1c01586DOI Listing
July 2021

Mass spectrometry-based urinary metabolomics for exploring the treatment effects of Radix ginseng-Schisandra chinensis herb pair on Alzheimer's disease in rats.

J Sep Sci 2021 Aug 16;44(16):3158-3166. Epub 2021 Jul 16.

State Key Laboratory of Electroanalytical Chemistry, National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.

Herb pairs are the unique combinations of two relatively fixed herbs, intrinsically convey the basic idea of traditional Chinese medicine prescriptions. The compatibility of Radix ginseng and Schisandra chinensis has been used in traditional Chinese medicine for treating Alzheimer's disease for many years. However, there are few studies on Radix ginseng-Schisandra chinensis herb pair, and the underlying action mechanism is still unclear. In this study, the mechanism of Radix ginseng-Schisandra chinensis herb pair on Alzheimer's disease was investigated by using the mass spectrometry-based urinary metabolomics method. Sixteen urinary endogenous metabolites were identified as potential biomarkers. Meanwhile, 10 biomarkers were quantified with tandem mass spectrometry. The study result showed that the brain pathologic symptoms of model rats were improved and the potential biomarkers were adjusted backward significantly after the herb pair administration. The metabolic pathways linked to the herb pair-regulated endogenous biomarkers included phenylalanine and tyrosine metabolism, tryptophan metabolism, purine metabolism, and so on. The above metabolic pathways reflected that Radix ginseng-Schisandra chinensis herb pair mainly regulates abnormal energy metabolism, reduces inflammation, and regulates gut microbiota and neurotransmitters in the treatment of Alzheimer's disease.
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http://dx.doi.org/10.1002/jssc.202100061DOI Listing
August 2021

Comparison of dynamic flow interaction methods between pipe system and overland in urban flood analysis.

Sci Rep 2021 06 8;11(1):12079. Epub 2021 Jun 8.

Research Center on Flood & Drought Disaster Reduction of the Ministry of Water Resources, China Institute of Water Resources and Hydropower Research, Yuyuantan South Road, Haidian District, Beijing, China.

Urban flooding can be predicted by using different modeling approaches. This study considered different methods of modeling the dynamic flow interactions between pipe systems and surface flooding in urban areas. These approaches can be divided into two categories based on surface runoff collection units. This paper introduces a new hydrodynamic model that couples the storm water management model and the 2D overland model. The model's efficiency was validated based on the aforementioned experimental dataset; agreement was verified by correlation values above 0.6. Additionally, this study used different approaches and compared their accuracy in predicting flooding patterns. The results show that the use of sub-catchments to model the collection of surface runoff was not predictive of the inundation process, indicating a lower goodness of fit with the recorded values than that of adopting cells. Moreover, to determine which method of adopting cells to collect runoff could better predict rainstorm-induced inundation, an error and correlation analysis was conducted. The analysis found low error and high correlation, suggesting that inundation can be effectively predicted by the new approaches. Ultimately, this study contributes to existing work on numerical analysis of the interaction methods of urban flooding.
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http://dx.doi.org/10.1038/s41598-021-88246-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187352PMC
June 2021

Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma.

J Ethnopharmacol 2021 Oct 4;278:114302. Epub 2021 Jun 4.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China. Electronic address:

Ethnopharmacological Relevance: Asthma is a chronic inflammatory disease, characterized by airway inflammation, hyperresponsiveness, and bronchial smooth muscle contraction. Qingfei Xiaoyan Wan (QFXYW), a traditional Chinese formula, has been shown to exert anti-asthma effects and immune response in multiple diseases.

Aim Of This Study: In this study, we evaluated the therapeutic mechanism of QFXYW in the suppression of allergic asthma by integrating of transcriptomics and system pharmacology.

Materials And Methods: BALB/c mice were sensitized with ovalbumin (OVA) to establish the allergic asthma model, and its success was confirmed with behavioral observations. Lung histopathological analysis, inflammatory pathology scores, transcription factors were used to evaluate the effects of QFXYW on allergic asthma. The therapeutic mechanism of QFXYW in treating allergic asthma through integrated transcriptomics and system pharmacology was then determined: hub genes were screened out by topological analysis and functional enrichment analysis were performed to identify key signaling pathway. Subsequently, quantitative RP-PCR and protein array were performed to detect the mRNA of hub genes and to predict the key pathway in OVA-induced allergic asthma, respectively.

Results: Our results demonstrated that QFXYW could significantly attenuate inflammatory cell infiltration, mucus secretion, and epithelial damage. The transcriptomics analysis found the six hub genes with the highest values- CXCL10, CXCL2, CXCL1, IL-6, CCL-5, and CCL-4 were screened out. Functional enrichment analysis showed that the differentially expressed genes (DEGs) were mainly enriched in the inflammatory response and cytokine signaling pathway. Moreover, the quantitative RT-PCR verification experiment found the CXCL2 and CXCL1 were significantly suppressed after treatment with QFXYW. The results of protein array showed that QFXYW inhibited the multi-cytokines of OVA-induced allergic asthma via cytokine signaling pathway.

Conclusions: QFXYW may have mediated OVA-induced allergic asthma mainly through the hub genes CXCL2, CXCL1, and the cytokine signaling pathway. This finding will offer a novel strategy to explore effective and safe mechanism of Traditional Chinese Medicine (TCM) formula to treat allergic asthma.
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http://dx.doi.org/10.1016/j.jep.2021.114302DOI Listing
October 2021

Study on the Inhibitory Effects of Naringenin-Loaded Nanostructured Lipid Carriers Against Nonalcoholic Fatty Liver Disease.

J Biomed Nanotechnol 2021 May;17(5):942-951

Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University, Beijing 100191, China.

Naringenin (NGN) can be used to inhibit the progression of nonalcoholic fatty liver disease (NAFLD) in mice, but its poor water solubility limits its applications. Nanostructured lipid carriers (NLCs) have recently attracted much attention in the field of nanodrug delivery systems because they increase the drug loading capacity and impressively enhance the solubility of indissolvable drugs. Herein, a thin-film dispersion method was used to prepare naringenin-loaded nanostructured lipid carriers (NGN-NLCs). These NGN-NLCs have a narrow size distribution of 171.9 ±2.0 nm, a high drug loading capacity of 23.7 ± 0.3%, a high encapsulation efficiency of 99.9 ± 0.0% and a drug release rate of 86.2 ± 0.4%. NGN- NLCs elevated the pharmacokinetic parameters (C and AUC) of NGN, accelerated NGN transepithelial transport in MDCK cells and intestinal absorption in the jejunum and ileum, and reduced hepatic lipid accumulation in an oleic acid (OA) plus lipopolysaccharide (LPS)-induced lipid deposition cell model in primary hepatocytes and in a methionine/choline deficient (MCD) diet-induced NAFLD mouse model. A detailed study of the mechanism showed that this NLC formulation elevated the drug release rate in simulated intestinal solutions the transepithelial transport in MDCK cells, the oral absorption in mice and the intestinal absorption of NGN. Thus, NGN-NLCs significantly enhanced the inhibitory effects of NGN on MCD diet induced mouse NAFLD.
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http://dx.doi.org/10.1166/jbn.2021.3077DOI Listing
May 2021

Identification of iron ore brands by multi-component analysis and chemometric tools.

Anal Bioanal Chem 2021 Jul 2;413(17):4521-4530. Epub 2021 Jun 2.

Technical Center for Industrial Product and Raw Material Inspection and Testing of Shanghai Customs, Shanghai, 200135, China.

Identification of iron ore brand is one of the most important precautions against fraud in the international iron ore trade. However, the identification of iron ore brand can be sophisticated, due to fact that the role played by multi-component in iron ore brand identification was unclear. This study aims to establish an objective approach to identify iron ore brands based on their multi-component content. A total of 1469 batches of iron ore samples, covering 16 commonly consumed iron ore brands from 3 countries, were analyzed for multi-component content. It was investigated that 10 primary, minor, and trace chemical components varied significantly in contents according to different iron ore brands. This prospective relationship between the multi-component contents and the iron ore brand was then used to place 16 brands into 12 groups and 8 brands of them were correctly identified by a flowchart. Furthermore, chemometric tools such as linear discriminant analysis (LDA), k-nearest neighbor (k-NN), and support vector machine (SVM) were applied to construct models to simultaneously discriminate 16 iron ore brands. Both the training and test results proved that LDA performed best in this circumstance. In the LDA method, MgO, Fe, SiO, and P are the feature components contributing the most to the identification of 16 brands of iron ore. Based on the findings, the multi-components are distinct variables to establish an internationally recognized model of iron ore brand identification.
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http://dx.doi.org/10.1007/s00216-021-03422-4DOI Listing
July 2021

Scale-Up Preparation of Crocins I and II from by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase.

Molecules 2021 May 24;26(11). Epub 2021 May 24.

College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China.

Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of , which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC values were determined to be 36.3 ± 6.24 and 29.7 ± 7.41 μM, respectively.
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http://dx.doi.org/10.3390/molecules26113137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197369PMC
May 2021

Spatio-temporal analysis of urban air pollutants throughout China during 2014-2019.

Air Qual Atmos Health 2021 May 14:1-14. Epub 2021 May 14.

Department of Environmental Health, School of Public Health, Key Laboratory of Environmental Health Damage Research and Assessment, China Medical University, Shenyang, 110122 Liaoning Province China.

Air pollution control has become the top priority of China's "green development" concept since 2013. The Chinese government has enacted a range of policies and statutes to control contaminant emissions and improve air quality. On the basis of the national air quality ground observation database, the spatial and temporal distribution of air quality index value (AQI), fine particulate matter (PM), coarse particles (PM), sulfur dioxide (SO), nitrogen dioxide (NO), carbon monoxide (CO), and ozone (O) were explored in 336 cities throughout China from 2014 to 2019. AQI and most pollutants (except O) decreased in concentrations from 2014 to 2019. In 2019, all cities except Henan reached the level 2 of the ambient air quality index, and six cities had a lower ambient air quality index and reached the level 1. Spatially, higher pollutant concentrations were concentrated in large city clusters, whereas the areas with high O concentration were found across the country. Furthermore, central heating was shown to have a negative impact on air quality. The observed AQI value, PM, PM, SO, NO, and CO concentrations were highest in north and northwest China and Henan province in central China. The correlations among pollutants suggest that the main sources of pollutants are fossil fuel combustion, industrial production, and motor vehicle emissions. The influence of meteorological factors on air quality, long-distance transportation, and the transformations of pollutants should be explored in future research.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11869-021-01043-5.
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http://dx.doi.org/10.1007/s11869-021-01043-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121134PMC
May 2021

Interpretable deep learning-assisted laser-induced breakdown spectroscopy for brand classification of iron ores.

Anal Chim Acta 2021 Jun 28;1166:338574. Epub 2021 Apr 28.

Technical Center for Industrial Product and Raw Material Inspection and Testing of Shanghai Customs, Shanghai, 200135, PR China. Electronic address:

Brand classification of iron ores using laser-induced breakdown spectroscopy (LIBS) combined with artificial neural networks can quickly realize the compliance verification and guarantee the interests of both trading partners. However, its practical application is impeded by complex pretreatments and unexplained feature learning problems. According to the LIBS data characteristics of iron ores, a convolutional neural network (CNN) is designed to predict 16 types of brand iron ores from Australia, Brazil, and South Africa. The accuracies of the calibration set and the prediction set with five-fold cross-validation (5-CV) were 99.86% and 99.88%, and the value of loss function was 0.0356. Meanwhile, the established CNN method was also compared with common machine learning methods using raw spectra as input variables, and it outperformed other methods. For the first time, this work interprets the CNN's effectiveness layer by layer in self-adaptively extracting LIBS features through t-distributed stochastic neighbor embedding (t-SNE) and the quantitative data of major chemical components in iron ores. Our approach shows that deep learning assisted LIBS is able to significantly reduce manual factors in preprocessing and feature selection and has broad application prospects in the brand classification of iron ores.
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http://dx.doi.org/10.1016/j.aca.2021.338574DOI Listing
June 2021

Cone-beam Computed Tomography-based three-dimensional cephalometric analysis of mandible symmetry and the occlusal plane of adult patients with high-angle skeletal class III malocclusion and jaw deformity.

Arch Oral Biol 2021 Jul 14;127:105157. Epub 2021 May 14.

Department of Dentomaxillofacial Radiology, Nanjing Stomatological Hospital, Medical School of Nanjing University, 210008, Nanjing, China. Electronic address:

Objective: To use cone beam computed tomography (CBCT) scans to analyze mandibular symmetry, the occlusal plane and their correlations in patients with high-angle skeletal class III malocclusion and jaw deformity.

Design: Reconstructed images simulated of CBCT data of 20 adult patients with high-angle skeletal class III malocclusion and jaw asymmetry (Experimental group) and 20 adult patients of skeletal class I with normal faces (Control group) were measured with a three-dimensional (3D) geometric morphological measurement system. Related landmarks, lines and planes were marked and relevant distances and angles of 3D craniofacial structures were measured. The Student t-test was performed to assess symmetry. With the help of the Pearson correlation and Linear regression, we explored the correlations and other relationships among them.

Results: Compared with control group, the symmetrical differences in experimental group were concentrated in condyle, coracoid process, mandibular angle, mandibular first molar area and the chin (P < 0.05). A strong positive correlation was found between the degree of mandibular deviation and the inclination of the occlusal plane in the coronal position (r = 0.860, P < 0.001) and a linear relationship between them was found: Y = 1.300 + 0.419X (the inclination of occlusal plane was Y, and the mandibular deviation was X).

Conclusions: Certain characteristics of mandibular symmetry and the occlusal plane were found in patients with high-angle skeletal class III malocclusion and jaw asymmetry. These findings provide references for doctors to pay attention to the degree and location of mandibular asymmetry and to determine the occlusal plane.
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http://dx.doi.org/10.1016/j.archoralbio.2021.105157DOI Listing
July 2021
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