Publications by authors named "Shiyi Chen"

268 Publications

Magnetic nanocarriers as a therapeutic drug delivery strategy for promoting pain-related motor functions in a rat model of cartilage transplantation.

J Mater Sci Mater Med 2021 Mar 31;32(4):37. Epub 2021 Mar 31.

Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China.

Cartilage is an avascular tissue with low cellularity and insufficient self-repair response. In clinical practice, a large articular cartilage defect is usually fixed by cartilage transplantation. Importantly, the fast repair process has been demanded postoperatively in the area between the host cartilage and the transplanted cartilage. In the past few years, magnetic nanoparticles have drawn great attention due to their biocompatible, biodegradable, and nontoxic properties. In addition, the nanoparticles can easily pass through the cell plasma membrane and increase the cellular uptake efficiency. Here, a therapeutic drug delivery strategy was proposed for cartilage repair. The prepared kartogenin (KGN)-conjugated magnetic nanocarriers (KGN@NCs) promoted the viability of chondrocytes in vitro. In a rat model of cartilage transplantation, intra-articularly delivered KGN@NCs generated cartilage with a flat surface and a high level of aggrecan in vivo. Notably, KGN@NCs were also capable of improving the pain-related motor functions. They promoted the motor functional parameters including the print area and intensity to restore to a normal level compared with the single KGN. Therefore, these therapeutic drug nanocarriers provided the potential for cartilage repair.
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http://dx.doi.org/10.1007/s10856-021-06508-8DOI Listing
March 2021

Surface modification of the simvastatin factor-loaded silk fibroin promotes the healing of rotator cuff injury through β-catenin signaling.

J Biomater Appl 2021 Mar 28:885328221995926. Epub 2021 Mar 28.

159397Huashan Hospital Fudan University, Shanghai, China.

Rupture of the rotator cuff is a common injury of the shoulder joint in sports professionals. In addition, research on repair of the rotator cuff has gained popularity over the recent years. Given the high rate of re-tear after surgery, it is necessary to design and prepare biodegradable materials with good mechanical properties, for the management of the condition. Consequently, the present study conducted surface modification of the simvastatin factor-loaded silk fibroin for the repair of chronic rotator cuff injury in SD rats. The experiments were analyzed through scanning electron microscopy and the water contact angle. Additionally, the CCK-8 assay was used to observe the effect of the intervention on the proliferation of BMSCs. Moreover, the osteogenic differentiation of BMSCs was detected through the ALP and ARS assays while the expression of osteogenic genes was examined using qRT-PCR and Western blot analysis. Furthermore, a model for repairing chronic rotator cuff tears in SD rats was established . Thereafter, rotator cuff repair and healing were evaluated through HE staining while Masson and Sirius staining was used to detect the collagen formation ratio. Additionally, the study analyzed the mechanism underlying the effect of simvastatin-loaded silk fibroin. The results showed that the simvastatin-loaded silk fibroin membrane had better biocompatibility and the experiments confirmed that it could promote the proliferation and osteogenic differentiation of BMSCs. In addition, the HE staining experiments similarly confirmed that it could enhance tendon bone healing and alleviate inflammation in chronic rotator cuff injuries. On the other hand, Masson and Sirius staining showed that the simvastatin-loaded silk fibroin could promote the formation of collagen. Further analysis also revealed that it could promote the osteogenic differentiation of BMSCs by activating the β-catenin signaling pathway. In general, these findings suggested that surface modification of the simvastatin factor-loaded silk fibroin was a potential means of improving the healing of rotator cuff injuries and can be implemented in clinical practice in future.
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http://dx.doi.org/10.1177/0885328221995926DOI Listing
March 2021

[Effectiveness of proximal femoral nail anti-rotation for high plane intertrochanteric femur fractures].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Mar;35(3):307-311

Department of Orthopedics, Yangpu Hospital, Tongji University, Shanghai, 200090, P.R.China.

Objective: To evaluate the effectiveness of proximal femoral nail anti-rotation (PFNA) in treatment of high plane intertrochanteric femur fractures.

Methods: A retrospective analysis was performed on 33 patients who underwent closed reduction and PFNA fixation for high plane intertrochanteric femur fracture between January 2016 and June 2019. There were 12 males and 21 females with an average age of 75.1 years (mean, 47-89 years). The fractures were caused by falling from height in 21 cases, by traffic accident in 7 cases, and by other injuries in 5 cases. Fractures were classified as type A in 14 cases and type B in 19 cases according to self-defined fracture classification criteria; and as type 31-A1.2 in 14 cases and as type 31-A2.2 in 19 cases according to AO/Orthopedic Trauma Association (AO/OTA) classification criteria. The time from injury to operation was 2-5 days (mean, 2.7 days). The operation time, intraoperative blood loss, hospital stay, fracture reduction quality, fracture healing time, internal fixation failure, and Parker-Palmer score were recorded.

Results: The operation time was 40-75 minutes (mean, 55 minutes). The intraoperative blood loss was 50-150 mL (mean, 64 mL). The hospital stay was 5-15 days (mean, 8.7 days). All incisions healed by first intention. Twenty-eight patients were followed up 12-18 months with an average of 13.6 months. The fracture reduction quality was rated as excellent in 9 cases (32.1%), good in 17 cases (60.7%), and poor in 2 cases (7.1%) by Chang's criteria. Parker-Palmer score was 6-9 (mean, 7.9) at last follow-up.

Conclusion: High plane intertrochanteric femur fracture is a special type of intertrochanteric fracture, which can be diagnosed by imaging examination. PFNA fixation can achieve satisfactory results and prevent the occurrence of internal fixation failure effectively.
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http://dx.doi.org/10.7507/1002-1892.202007114DOI Listing
March 2021

Comparison of the Prognostic Ability of the HCT-CI, the Modified EBMT, and the EBMT-ADT Pre-transplant Risk Scores for Acute Leukemia.

Clin Lymphoma Myeloma Leuk 2021 Feb 3. Epub 2021 Feb 3.

Hans Messner Allogeneic Transplant Program, Princess Margaret Cancer Centre, Toronto, Ontario, Canada. Electronic address:

Background: Allogeneic hematopoietic cell transplantation (HCT) outcomes may be predicted by published risk scores; however, the ideal system has not been identified for acute leukemias.

Patients And Methods: We retrospectively examined the Hematopoietic Cell Transplantation-Comorbidity Index (HCT-CI), modified European Group for Blood and Marrow Transplantation (mEBMT), EBMT-Alternating Decision Tree (ADT), and others on 231 patients with acute leukemia.

Results: Acute myeloid leukemia was diagnosed in 200 patients, and acute lymphocytic leukemia was diagnosed in 31 patients. For HCT-CI, patients were grouped as 0 to 1, 2 to 3, and > 3. For mEBMT, patients were grouped as 0 to 2, 3, and > 3. For EBMT-ADT, the 100-day mortality was calculated and grouped as ≤ 4.1%, 4.1% to 11.5%, and > 11.5%. Higher HCI-CI demonstrated inferior overall survival (P = .04; c-statistic, 0.57), whereas mEBMT and EBMT-ADT did not stratify well. A new weighted score was developed that assigned 1 point for age ≥ 60 years, acute lymphocytic leukemia diagnosis, mismatch unrelated or haploidentical donor, cardiovascular comorbidity, and pre-transplant diabetes, whereas arrhythmia received 2 points. The new weighted score assigned 0 points to 88 (38%), 1 to 2 points to 121 (52%) and ≥ 3 points to 22 (10%) patients, and demonstrated improved prognostic capability compared with the other scores (P = .0001; c-statistic, 0.61).

Conclusions: The HCT-CI stratifies patients with leukemia for overall survival but is inferior to our single-center score, which is influenced by cardiac comorbidity and arrhythmia. Differences in pre-transplant risk scores may be related to different transplant practices.
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http://dx.doi.org/10.1016/j.clml.2021.01.022DOI Listing
February 2021

Conditioned medium of human bone marrow-derived stem cells promotes tendon-bone healing of the rotator cuff in a rat model.

Biomaterials 2021 Feb 11;271:120714. Epub 2021 Feb 11.

Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China. Electronic address:

Rotator cuff repair is a common surgery in sports medicine. During the surgery, torn tendon was re-fixed onto the bony surface. The majority of patients gain good results. However, re-tear occurs in some patients. The reason under this phenomenon is that the normal tendon-bone enthesis cannot be reconstructed. In order to strengthen the tendon-bone healing and promote enthesis regeneration, numerous manners are tested, among which stem cell related therapies are preferred. Stem cells, due to the ability of multi-lineage differentiation, are widely used in regenerative medicine. However, safety and ethics concerns limit its clinical use. Recent studies found that it is the secretome of stem cells that is biologically effective. On ground of this, we, in the current study, collected the conditioned medium of human bone marrow-derived stem cells (hBMSC-CM) and tested whether this acellular method could promote tendon-bone healing in a rat model of rotator cuff repair. By using histological, radiological, and biomechanical methods, we found that hBMSC-CM promoted tendon-bone healing of the rat rotator cuff. Then, we noticed that hBMSC-CM exerted an impact on macrophage polarization both in vivo and in vitro by inhibiting M1 phenotype and promoting M2 phenotype. Further, we proved that the benefit of hBMSC-CM on tendon-bone healing was related to its regulation on macrophage. Finally, we proved that, hBMSC-CM influenced macrophage polarization, which was, at least partially, related to Smad2/3 signaling pathway. Based on the experiments above, we confirmed the benefit of hBMSC-CM on tendon-bone healing, which relied on its immune-regulative property. Considering the accessibility and safety of acellular hBMSC-CM, we believe it is a promising candidate clinically for tendon-bone healing.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120714DOI Listing
February 2021

Observations and modeling of OH and HO radicals in Chengdu, China in summer 2019.

Sci Total Environ 2021 Jun 27;772:144829. Epub 2021 Jan 27.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, China; International Joint laboratory for Regional pollution Control (IJRC), Peking University, Beijing, China; Beijing Innovation Center for Engineering Sciences and Advanced Technology, Peking University, Beijing, China; CAS Center for Excellence in Regional Atmospheric Environment, Chinese Academy of Science, Xiamen, China. Electronic address:

This study reports on the first continuous measurements of ambient OH and HO radicals at a suburban site in Chengdu, Southwest China, which were collected during 2019 as part of a comprehensive field campaign 'CompreHensive field experiment to explOre the photochemical Ozone formation mechaniSm in summEr - 2019 (CHOOSE-2019)'. The mean concentrations (11:00-15:00) of the observed OH and HO radicals were 9.5 × 10 and 9.0 × 10 cm, respectively. To investigate the state-of-the-art chemical mechanism of radical, closure experiments were conducted with a box model, in which the RACM2 mechanism updated with the latest isoprene chemistry (RACM2-LIM1) was used. In the base run, OH radicals were underestimated by the model for the low-NO regime, which was likely due to the missing OH recycling. However, good agreement between the observed and modeled OH concentrations was achieved when an additional species X (equivalent to 0.25 ppb of NO mixing ratio) from one new OH regeneration cycle (RO + X → HO, HO + X → OH) was added into the model. Additionally, in the base run, the model could reproduce the observed HO concentrations. Discrepancies in the observed and modeled HO concentrations were found in the sensitivity runs with HO heterogeneous uptake, indicating that the impact of the uptake may be less significant in Chengdu because of the relatively low aerosol concentrations. The ROx (= OH + HO + RO) primary source was dominated by photolysis reactions, in which HONO, O, and HCHO photolysis accounted for 34%, 19%, and 23% during the daytime, respectively. The efficiency of radical cycling was quantified by the radical chain length, which was determined by the NO to NO ratio successfully. The parameterization of the radical chain length may be very useful for the further determinations of radical recycling.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144829DOI Listing
June 2021

Cross-Subject Assistance: Inter- and Intra-Subject Maximal Correlation for Enhancing the Performance of SSVEP-Based BCIs.

IEEE Trans Neural Syst Rehabil Eng 2021 2;29:517-526. Epub 2021 Mar 2.

Objective: The current state-of-the-art methods significantly improve the detection performance of the steady-state visual evoked potentials (SSVEPs) by using the individual calibration data. However, the time-consuming calibration sessions limit the number of training trials and may give rise to visual fatigue, which weakens the effectiveness of the individual training data. For addressing this issue, this study proposes a novel inter- and intra-subject maximal correlation (IISMC) method to enhance the robustness of SSVEP recognition via employing the inter- and intra-subject similarity and variability. Through efficient transfer learning, similar experience under the same task is shared across subjects.

Methods: IISMC extracts subject-specific information and similar task-related information from oneself and other subjects performing the same task by maximizing the inter- and intra-subject correlation. Multiple weak classifiers are built from several existing subjects and then integrated to construct the strong classifiers by the average weighting. Finally, a powerful fusion predictor is obtained for target recognition.

Results: The proposed framework is validated on a benchmark data set of 35 subjects, and the experimental results demonstrate that IISMC obtains better performance than the state of the art task-related component analysis (TRCA).

Significance: The proposed method has great potential for developing high-speed BCIs.
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http://dx.doi.org/10.1109/TNSRE.2021.3057938DOI Listing
March 2021

Analysis of , and Genes Polymorphism in Four Rabbit Breeds with Different Coat Colors.

Animals (Basel) 2021 Jan 5;11(1). Epub 2021 Jan 5.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

Pigmentation genes such as , and play a major role in rabbit coat color. To understand the genotypic profile underlying coat color in indigenous Chinese rabbit breeds, portions of the above-mentioned genes were amplified and variations in them were analyzed by DNA sequencing. Based on the analysis of 24 Tianfu black rabbits, 24 Sichuan white rabbits, 24 Sichuan gray rabbits, and 24 Fujian yellow rabbits, two indels in , three SNPs in , five SNPs (single nucleotide polymorphisms) in , one SNP in , and three SNPs in were discovered. These variations have low-to-moderate polymorphism, and there are significant differences in their distribution among the different breeds ( < 0.05). These results provide more information regarding the genetic background of these native rabbit breeds and reveal their high-quality genetic resources.
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http://dx.doi.org/10.3390/ani11010081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824738PMC
January 2021

Organic Iodine Compounds in Fine Particulate Matter from a Continental Urban Region: Insights into Secondary Formation in the Atmosphere.

Environ Sci Technol 2021 Feb 14;55(3):1508-1514. Epub 2021 Jan 14.

State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, P. R. China.

Atmospheric iodine chemistry can significantly affect the atmospheric oxidation capacity in certain regions. In such processes, particle-phase organic iodine compounds (OICs) are key reservoir species in their loss processes. However, their presence and formation mechanism remain unclear, especially in continental regions. Using gas chromatography and time-of-flight mass spectrometry coupled with both electron capture negative ionization and electron impact sources, this study systematically identified unknown OICs in 2-year samples of ambient fine particulate matter (PM) collected in Beijing, an inland city. We determined the molecular structure of 37 unknown OICs, among which six species were confirmed by reference standards. The higher concentrations for ∑OICs (median: 280 pg m; range: 49.0-770 pg m) measured in the heating season indicate intensive coal combustion sources of atmospheric iodine. 1-Iodo-2-naphthol and 4-iodoresorcinol are the most abundant species mainly from primary combustion emission and secondary formation, respectively. The detection of 2- and 4-iodoresorcinols, but not of iodine-substituted catechol/hydroquinone or 5-iodoresorcinol, suggests that they are formed via the electrophilic substitution of resorcinol by hypoiodous acid, a product of the reaction of iodine with ozone. This study reports isomeric information on OICs in continental urban PM and provides valuable evidence on the formation mechanism of OICs in ambient particles.
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http://dx.doi.org/10.1021/acs.est.0c06703DOI Listing
February 2021

Whole-genome resequencing reveals loci with allelic transmission ratio distortion in F chicken population.

Mol Genet Genomics 2021 Mar 6;296(2):331-339. Epub 2021 Jan 6.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, 611130, China.

Allelic transmission ratio distortion (TRD) is the significant deviation from the expected ratio under Mendelian inheritance theory, which may be resulted from multiple disrupted biological processes, including germline selection, meiotic drive, gametic competition, imprint error, and embryo lethality. However, it is less known that whether or what extent the allelic TRD is present in farm animals. In this study, whole-genome resequencing technology was applied to reveal TRD loci in chicken by constructing a full-sib F hybrid population. Through the whole-genome resequencing data of two parents (30 ×) and 38 offspring (5 ×), we detected a total of 2850 TRD SNPs (p-adj < 0.05) located within 400 genes showing TRD, and all of them were unevenly distributed on macrochromosomes and microchromosomes. Our findings suggested that TRD in the chicken chromosome 16 might play an important role in chicken immunity and disease resistance and the MYH1F with significant TRD and allele-specific expression could play a key role in the fast muscle development. In addition, functional enrichment analyses revealed that many genes (e.g., TGFBR2, TGFBR3, NOTCH1, and NCOA1) with TRD were found in the significantly enriched biological process and InterPro terms in relation to embryonic lethality and germline selection. Our results suggested that TRD is considerably prevalent in the chicken genome and has functional implications.
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http://dx.doi.org/10.1007/s00438-020-01744-zDOI Listing
March 2021

Humidity-Dependent Phase State of Gasoline Vehicle Emission-Related Aerosols.

Environ Sci Technol 2021 01 30;55(2):832-841. Epub 2020 Dec 30.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, International Joint Laboratory for Regional Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

The phase states of primarily emitted and secondarily formed aerosols from gasoline vehicle exhausts were investigated by quantifying the particle rebound fraction (). The rebound behaviors of gasoline vehicle emission-related aerosols varied with engines, fuel types, and photochemical aging time, showing distinguished differences from biogenic secondary organic aerosols. The nonliquid-to-liquid phase transition of primary aerosols emitted from port fuel injection (PFI) and gasoline direct injection (GDI) vehicles started at a relative humidity (RH) = 50 and 60%, and liquefaction was accomplished at 60 and 70%, respectively. The RH at which declined to 0.5 decreased from 70 to 65% for the PFI case with 92# fuel, corresponding to the photochemical aging time from 0.37 to 4.62 days. For the GDI case, such RH enhanced from 60 to 65%. Our results can be used to imply the phase state of traffic-related aerosols and further understand their roles in urban atmospheric chemistry. Taking Beijing, China, as an example, traffic-related aerosols were mainly nonliquid during winter with the majority ambient RH below 50%, whereas they were mostly liquid during the morning rush hour of summer, and traffic-related secondary aerosols fluctuated between nonliquid and liquid during the daytime and tended to be liquid at night with increased ambient RH.
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http://dx.doi.org/10.1021/acs.est.0c05478DOI Listing
January 2021

A novel algorithm to determine the scattering coefficient of ambient organic aerosols.

Environ Pollut 2021 Feb 2;270:116209. Epub 2020 Dec 2.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, International Joint Laboratory for Regional Pollution Control (IJRC), Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, PR China.

In the present work, we propose a novel algorithm to determine the scattering coefficient of OA by evaluating the relationships of the MSEs for primary organic aerosol (POA) and secondary organic aerosol (SOA) with their mass concentrations at three distinct sites, i.e. an urban site, a rural site, and a background site in China. Our results showed that the MSEs for POA and SOA increased rapidly as a function of mass concentration in low mass loading. While the increasing rate declined after a threshold of mass loading of 50 μg/m for POA, and 15 μg/m for SOA, respectively. The dry scattering coefficients of submicron particles (PM) were reconstructed based on the algorithm for POA and SOA scattering coefficient and further verified by using multi-site data. The calculated dry scattering coefficients using our reconstructing algorithm have good consistency with the measured ones, with the high correlation and small deviation in Shanghai (R = 0.98; deviations: 2.9%) and Dezhou (R = 0.90; deviations: 4.7%), indicating that our algorithms for OA and PM are applicable to predict the scattering coefficient of OA and Submicron particle (PM) in China.
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http://dx.doi.org/10.1016/j.envpol.2020.116209DOI Listing
February 2021

Treatment of Isolated Posterolateral Tibial Plateau Fracture with a Horizontal Belt Plate through the Anterolateral Supra-Fibular-Head Approach.

Biomed Res Int 2020 3;2020:4186712. Epub 2020 Dec 3.

Department of Orthopaedic Surgery, Yangpu Hospital, Tongji University School of Medicine, 450 Tengyue Road, Shanghai 200090, China.

The posterolateral tibial plateau fracture was not easy to be exposed and fixed with usual techniques. The aim of this study was to investigate the biomechanical stability and clinical outcome of the isolated posterolateral tibial plateau fracture fixed with a single horizontal belt plate through the anterolateral supra-fibular-head approach. Fracture models were created by 18 synthetic tibias and fixed with three different fixation modes. Each group was fixed and tested on the loading machine, and final vertical displacement of the fragment was detected and calculated. Clinically, a retrospective analysis of 12 cases of posterolateral tibial plateau fracture from January 2013 to December 2017 was performed. There were 8 males and 4 females, aged 33-72 years, with an average age of 49.6 years. Isolated posterolateral tibial plateau fractures were identified according to preoperative X-ray and computed tomography scan. Through the modified anterolateral supra-fibular-head approach, the fracture was reduced and fixed by a prebending T-shaped distal radius plate and rafting screws, with bone substitute grafting or autogenous iliac bone implantation. Patients were followed up to a minimum one year of time period, and the outcome was evaluated clinically and radiologically. The biomechanical study shows that horizontal belt plate fixation for the isolated PL tibial plateau fracture can provide sufficient stability, allowing early knee functional exercise and partial weight bearing. For clinical case series, the average operation time in this group was 73.3 ± 10.2 mins (range: 55-90), and the average duration of hospitalization was 9.1 ± 3.3 days (range: 5-16). Patients were followed up for 12-24 months with an average of 16.5 months, and all patients achieved radiological fracture union after an average of 13.7 weeks. At one year after operation, the average knee score of the Hospital for Special Surgery (HSS) scale was 93.2 ± 4.2 points(range: 90-98), the average score of SMFA was 21.1 ± 5.6 points (range: 14-31), and the average knee range of motion (ROM) was 121.48° ± 8.88° (range: 105°-135°). There were 8 cases that were very satisfied and 3 cases that were satisfied with the operation. For an isolated posterolateral tibial plateau fracture, the supra-fibular-head approach can fully expose the fracture site; the horizontal belt plate fixation of the fracture is stable and reliable to allow for early-stage knee rehabilitation, and the outcome of medium-term clinical follow-up was satisfactory.
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http://dx.doi.org/10.1155/2020/4186712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728474PMC
December 2020

Antibacterial Mechanism of Silkworm Seroins.

Polymers (Basel) 2020 Dec 14;12(12). Epub 2020 Dec 14.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.

Seroin 1 and seroin 2 are abundant in silkworm cocoon silk and show strong antibacterial activities, and thus are thought to protect cocoon silk from damage by bacteria. In this study, we characterized the expression pattern of silkworm seroin 3, and found that seroin 3 is synthesized in the female ovary and secreted into egg to play its roles. After being infected, seroin 1, 2, and 3 were significantly up-regulated in the silkworm. We synthesized the full-length protein of seroin 1, 2, and 3 and their N/C-terminal domain (seroin-N/C), and compared the antimicrobial activities in vitro. All three seroins showed higher antibacterial activity against Gram-positive bacteria than against Gram-negative bacteria. Seroin 2 showed better antibacterial effect than seroin 1 and 3, whereas seroin 1/2/3-N was better than seroin 1/2/3-C. We found that seroin 2-C has stronger peptidoglycan binding ability than seroin 2-N per the ELISA test. The binding sites of seroin 2 with bacteria were blocked by peptidoglycan, which resulted in the loss of the antibacterial activity of seroin 2. Collectively, these findings suggest that seroin 1 and 2 play antibacterial roles in cocoon silk, whereas seroin 3 functions in the eggs. The three silkworm seroins have the same antibacterial mechanism, that is, binding to bacterial peptidoglycan by the C-terminal domain and inhibiting bacterial growth by the N-terminal domain.
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http://dx.doi.org/10.3390/polym12122985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765120PMC
December 2020

Analysis of the immunoglobin E molecular sensitization profile in children with allergic asthma and predictive factors for the efficacy of allergy immunotherapy.

Ann Transl Med 2020 Nov;8(21):1459

Department of Respiratory Medicine Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China.

Background: As the incidence of allergic asthma in children increases, the immunoglobin E (IgE) molecular sensitization profile of allergic asthma remains underreported while the level of total IgE (tIgE) and sIgE/tIgE have not been studied as predictors of efficacy of allergen immunotherapy (AIT) for allergic asthma, specifically in children.

Methods: Starting from August 2018 to March 2019 in the Respiratory Department of the Children's Hospital of Chongqing Medical University, asthmatic children, with positive skin prick tests to Der p or Der f, were enrolled in this study. Total IgE, allergen serum-specific IgE (sIgE) of Der p, Der f, (Blo t), cat dander, dog dander, egg white, milk, cockroach, shrimp, and crab, along with Der p allergen components, Der p1 and Der p2, were measured by ImmunoCAP™ assay.

Results: A total of 142 children with allergic asthma were enrolled, all of whom showed positive IgE for Der p, Der p1, Der p2, and Der f; meanwhile, the positive rates of Blo t, cat dander, dog dander, egg white, milk, cockroach, shrimp, and crab were 91.84%, 10.96%, 7.32%, 9.15%, 11.58%, 17.03%, 18.90%, and 18.28% respectively. A significantly high correlation was found between total IgE and the sIgE of Der f, Der p, Der p 1, and Der p 2. Asthmatic children with a family history of allergy displayed higher total IgE and unknown IgE levels than those patients without a family history of allergy. The ratios of Der f sIgE/tIgE and Der p sIgE/tIgE were higher in the negative family history of allergy group than in the positive family history of allergy group. Furthermore, total IgE and unknown IgE were higher in the polysensitized group than in the in monosensitized group. The ratios of Der f sIgE/tIgE and Der p sIgE/tIgE were higher in the monosensitized group than in the polysensitized group.

Conclusions: From this study, we noticed that dust mites are the main cause of asthma in children investigated. Our findings indicate patients with no family history of allergy and monosensitized patients have a higher ratio of sIgE/tIgE, and those patients may benefit more from AIT.
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http://dx.doi.org/10.21037/atm-20-7314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723661PMC
November 2020

Screening and Identification of Differentially Expressed and Adipose Growth-Related Protein-Coding Genes During the Deposition of Perirenal Adipose Tissue in Rabbits.

Diabetes Metab Syndr Obes 2020 1;13:4669-4680. Epub 2020 Dec 1.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.

Background: Rabbit is a good model for genetic and medical studies in other livestock species. The rabbit shows low adipose tissue deposition, and the phenomena indicates that there is some specificity of adipose deposition during the rabbit growth. However, little is known about genes that regulate the growth of adipose tissue in rabbits.

Materials And Methods: Deep RNA-seq and comprehensive bioinformatics analyses were used to characterize the genes of rabbit visceral adipose tissue (VAT) at 35, 85 and 120 days after birth. Differentially expressed genes (DEGs) were identified at the three growth stages by DESeq. To explore the function of the candidate genes, Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. Six DEGs were randomly selected, and their expression profiles were validated by q-PCR.

Results: A total of 20,303 known transcripts and 99,199 new transcripts from 8 RNA sequencing libraries were identified, and 34 differentially expressed genes (DEGs) were screened. GO enrichment and KEGG pathway analyses revealed that the DEGs were mainly involved in lipid metabolism regulation including acylglycerol metabolic process and mobilization, and decomposition of lipids to generate ATP in adipocytes and fatty acid metabolism, included and . In addition, 133 protein-coding genes that play a role in adipose growth and development were screened, including acyl-CoA synthetase long-chain family member 5 () and fatty acid-binding protein 2 (). The validation results of six DEGs by q-PCR showed similar trends with the results of RNA-seq.

Conclusion: In summary, this study provides the first report of the coding genes profiles of rabbit adipose tissue during different growth stages. These data allow for the identification of candidate genes for subsequent studies on rabbit genetics and regulation of adipose cells, and provide an animal model for studying obesity in humans.
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http://dx.doi.org/10.2147/DMSO.S284246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719053PMC
December 2020

Elucidating the effect of HONO on O pollution by a case study in southwest China.

Sci Total Environ 2021 Feb 25;756:144127. Epub 2020 Nov 25.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; International Joint Laboratory for Regional Pollution Control, Ministry of Education, Beijing 100816, China; Collaborative Innovation Centre of Atmospheric Environment and Equipment Technology, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Photolysis of nitrous acid (HONO) is one of the major sources for atmospheric hydroxyl radicals (OH), playing significant role in initiating tropospheric photochemical reactions for ozone (O) production. However, scarce field investigations were conducted to elucidate this effect. In this study, a field campaign was conducted at a suburban site in southwest China. The whole observation was classified into three periods based on O levels and data coverage: the serious O pollution period (Aug 13-18 as P1), the O pollution period (Aug 22-28 as P2) and the clean period (Sep 3-12 as P3), with average O peak values of 96 ppb, 82 ppb and 44 ppb, respectively. There was no significant difference of the levels of O precursors (VOCs and NOx) between P1 and P2, and thus the evident elevation of OH peak values in P1 was suspected to be the most possible explanation for the higher O peak values. Considering the larger contribution of HONO photolysis to HO primary production than photolysis of HCHO, O and ozonolysis of Alkenes, sensitivity tests of HONO reduction on O production rate in P1 are conducted by a 0-dimension model. Reduced HONO concentration effectively slows the O production in the morning, and such effect correlates with the calculated production rate of OH radicals from HONO photolysis. Higher HONO level supplying for OH radical initiation in the early morning might be the main reason for the higher O peak values in P1, which explained the correlation (R = 0.51) between average O value during daytime (10:00-19:00 LT) and average HONO value during early morning (00:00-05:00 LT). For nighttime accumulation, a suitable range of relative humidity that favored NO conversion within P1 was assumed to be the reason for the higher HONO concentration in the following early morning which promoted O peak values.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144127DOI Listing
February 2021

Relationship Between Age at Initial Shoulder Instability and Overall Outcomes After Arthroscopic Bankart Repair: Mean 6-Year Follow-up.

Orthop J Sports Med 2020 Nov 20;8(11):2325967120964881. Epub 2020 Nov 20.

Sports Medicine Institute of Fudan University, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Background: Age at surgery plays a crucial role in the frequency of recurrent shoulder instability. However, there are few studies that evaluate the relationship between age at initial shoulder instability and overall outcomes after stabilization surgery.

Purpose: To compare clinical outcomes and structural changes after arthroscopic Bankart repair in patients who experienced initial shoulder instability during adolescence versus those with later onset instability.

Study Design: Cohort study; Level of evidence, 3.

Methods: This study included patients who underwent arthroscopic Bankart repair at a single institution between 2007 and 2017. Comparisons were made between patients who experienced initial shoulder instability during adolescence (age 13-19 years; group A) and those with later onset instability (age 20-35 years; group B). Clinical outcomes (recurrence rate, postoperative pain, functional scores, active range of motion, and return to sports) and structural changes demonstrated by magnetic resonance imaging (MRI) were evaluated at minimum 2-year follow-up. In addition, functional outcomes within each group were compared between the patients with and without postoperative recurrence.

Results: A total of 58 patients were included (24 patients in group A and 34 patients in group B). The mean follow-up was 72.1 months. Group A demonstrated a significantly higher recurrence rate than group B (41.7% vs 11.8%, respectively; = .009; risk ratio, 5.36 [95% CI, 1.43-20.09]) as well as significantly lower Rowe (76.9 ± 20.1 vs 88.7 ± 13.2, respectively; = .01) and Constant-Murley scores (92.2 ± 7.6 vs 96.3 ± 4.2, respectively; = .01). Postoperative MRI revealed no significant structural differences between the groups regarding the glenoid labrum, glenohumeral cartilage, or osseous reaction around the implanted anchors. In group A, patients with recurrence had less satisfaction regarding postoperative sports level than those without recurrence, whereas in group B, patients with recurrence had more postoperative pain and functional impairment compared with those without recurrence.

Conclusion: Initial shoulder instability during adolescence was associated with a higher recurrence rate and lower functional scores after arthroscopic Bankart repair compared with later onset instability, although no significant structural differences were found between the groups on MRI at a mean 6-year follow-up.
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http://dx.doi.org/10.1177/2325967120964881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682236PMC
November 2020

Association between SIRT6 Methylation and Human Longevity in a Chinese Population.

Public Health Genomics 2020 25;23(5-6):190-199. Epub 2020 Nov 25.

Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, China.

Background: Sirtuin 6 gene (SIRT6) is a longevity gene that is involved in a variety of metabolic pathways, but the relationship between SIRT6 methylation and longevity has not been clarified.

Methods: We conducted a case-control study on 129 residents with a family history of longevity (1 of parents, themselves, or siblings aged ≥90 years) and 86 individuals without a family history of exceptional longevity to identify the association. DNA pyrosequencing was performed to analyze the methylation status of SIRT6 promoter CpG sites. qRT-PCR and ELISA were used to estimate the SIRT6 messenger RNA (mRNA) levels and protein content. Six CpG sites (P1-P6) were identified as methylation variable positions in the SIRT6 promoter region.

Results: At the P2 and P5 CpG sites, the methylation rates of the longevity group were lower than those of the control group (p < 0.001 and p = 0.009), which might be independent determinants of longevity. The mRNA and protein levels of SIRT6 decreased in the control group (p < 0.0001 and p = 0.038). The mRNA level negatively correlated with the methylation rates at the P2 (rs = -0.173, p = 0.011) and P5 sites (rs = -0.207, p = 0.002). Furthermore, the protein content positively correlated with the methylation rate at the P5 site (rs = 0.136, p = 0.046) but showed no significant correlation with the methylation rate at the P2 site.

Conclusion: The low level of SIRT6 methylation may be a potential protective factor of Chinese longevity.
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http://dx.doi.org/10.1159/000508832DOI Listing
November 2020

Cell-Free Bilayered Porous Scaffolds for Osteochondral Regeneration Fabricated by Continuous 3D-Printing Using Nascent Physical Hydrogel as Ink.

Adv Healthc Mater 2021 02 23;10(3):e2001404. Epub 2020 Nov 23.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438, China.

Cartilage is difficult to self-repair and it is more challenging to repair an osteochondral defects concerning both cartilage and subchondral bone. Herein, it is hypothesized that a bilayered porous scaffold composed of a biomimetic gelatin hydrogel may, despite no external seeding cells, induce osteochondral regeneration in vivo after being implanted into mammal joints. This idea is confirmed based on the successful continuous 3D-printing of the bilayered scaffolds combined with the sol-gel transition of the aqueous solution of a gelatin derivative (physical gelation) and photocrosslinking of the gelatin methacryloyl (gelMA) macromonomers (chemical gelation). At the direct printing step, a nascent physical hydrogel is extruded, taking advantage of non-Newtonian and thermoresponsive rheological properties of this 3D-printing ink. In particular, a series of crosslinked gelMA (GelMA) and GelMA-hydroxyapatite bilayered hydrogel scaffolds are fabricated to evaluate the influence of the spacing of 3D-printed filaments on osteochondral regeneration in a rabbit model. The moderately spaced scaffolds output excellent regeneration of cartilage with cartilaginous lacunae and formation of subchondral bone. Thus, tricky rheological behaviors of soft matter can be employed to improve 3D-printing, and the bilayered hybrid scaffold resulting from the continuous 3D-printing is promising as a biomaterial to regenerate articular cartilage.
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http://dx.doi.org/10.1002/adhm.202001404DOI Listing
February 2021

live imaging of bone using shortwave infrared fluorescent quantum dots.

Nanoscale 2020 Nov;12(43):22022-22029

Institute of Sports Medicine of Fudan University, Department of Orthopaedic Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.

Bone plays an increasingly critical role in human health and disease. More noninvasive multi-scale imaging techniques are urgently required for investigations on the substructures and biological functions of bones. Our results firstly revealed that SWIR QDs prepared by us acted as a bone-specific imaging contrast to achieve real-time observation of bone structures both in vivo and ex vivo. The major bone structures of both Balb/C nude mice and Balb/C mice including their skull, spine, pelvis, limbs, and sternum could be rapidly and gradually identified via blood circulation after QD injection in vivo. More importantly, the binding capability of our QDs mainly depended on the biological activities of bone tissues, suggesting that our technique is suitable for in vivo live imaging. In addition, the cell imaging results suggested that the potential mechanism of our bone imaging could be ascribed to the highly specific interaction between QDs and MC3T3-E1 cells. In a word, the skeletal structures and biological activities of bones are anticipated to be observed and monitored with this QD-guided SWIR imaging strategy, respectively. This radiation-free QD-guided SWIR live imaging of bone can add new insights into a comprehensive study of bones in vivo and provide a basis for early diagnosis of bone diseases.
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http://dx.doi.org/10.1039/d0nr06261hDOI Listing
November 2020

LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma.

Cancer Manag Res 2020 23;12:10521-10530. Epub 2020 Oct 23.

Department of Orthopaedic Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, People's Republic of China.

Purpose: The objective of this study was to detect the expression of the long noncoding RNA (lncRNA) colorectal cancer-associated lncRNA (CCAL) in osteosarcoma tissues and to investigate its role in angiogenesis and the potential molecular mechanisms associated with this effect in osteosarcoma.

Materials And Methods: CCAL expression in 40 osteosarcoma tissues and 40 noncancerous tissues was measured by qRT-PCR (quantitative real-time polymerase chain reaction). Tube formation assays were performed to explore the role of CCAL in angiogenesis in osteosarcoma. In addition, the regulatory interaction between CCAL, miR-29b, and ANGPTL4 was investigated via luciferase reporter assay and bioinformatics predictive analysis.

Results: Compared with noncancerous tissues, the expression of CCAL was markedly upregulated in osteosarcoma tissues. Higher CCAL expression levels were closely related to shorter overall survival in patients with osteosarcoma. Additionally, functional analysis indicated that CCAL could facilitate tumour angiogenesis in vitro and in vivo in osteosarcoma. Mechanistically, CCAL upregulated ANGPTL4 expression in osteosarcoma cells, and ANGPTL4 mediated angiogenic induction by CCAL in osteosarcoma. Moreover, CCAL directly targeted miR-29b in osteosarcoma. More importantly, we demonstrated that CCAL upregulated the expression of ANGPTL4 by sponging miR-29b, which promoted angiogenesis in osteosarcoma.

Conclusion: Our results show that CCAL promotes angiogenesis by regulating the miR-29b/ANGPTL4 axis in osteosarcoma.
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http://dx.doi.org/10.2147/CMAR.S272230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591080PMC
October 2020

Fresh vs. frozen allogeneic peripheral blood stem cell grafts: A successful timely option.

Am J Hematol 2021 02 10;96(2):179-187. Epub 2020 Nov 10.

Hans Messner Allogeneic Transplant Program, Princess Margaret Cancer Centre, University Health Network, University of Toronto, Toronto, Ontario, Canada.

Cryopreservation of grafts has been established in autologous and cord blood transplantation, yet there is little experience regarding the effect of cryopreservation with sibling and unrelated grafts. We evaluated the effect of cryopreservation of grafts on allogeneic transplant outcomes using related, unrelated and haploidentical donors, including 958 patients, age 18-74 years (median 55) and using PBSC for various hematologic malignancies. Fresh grafts were received by 648 (68%) patients, 310 (32%) received cryopreserved. There was no difference between fresh vs cryopreserved grafts for neutrophil engraftment (P = .09), platelet engraftment (P = .11), graft failure (5.6% vs 6.8%, P = .46) and grade II-IV acute graft-vs-host disease (GVHD) (P = .71), moderate/severe chronic GVHD was observed in 176 (27%) vs 123 (40%) patients, respectively (P < .001). Multivariable analysis demonstrated no difference between fresh vs cryopreserved for OS (P = .39) and CIR (P = .08) while fresh grafts demonstrated borderline increased NRM (HR 1.27, 95% CI 1.02-1.59, P = .04). Of note, for patients with no or mild chronic GVHD, CIR was less for fresh compared to cryopreserved (HR = 0.67 for fresh, 95% CI 0.48-0.92, P = .01). We conclude there were no differences in engraftment and survival between fresh and cryopreserved grafts for allogeneic HCT, thus establishing cryopreservation to be a safe option for allogeneic HCT.
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http://dx.doi.org/10.1002/ajh.26033DOI Listing
February 2021

Outcomes of adult patients with acute myeloid leukemia and unsuccessful cytogenetic analysis undergoing allogeneic hematopoietic stem cell transplantation.

Hematol Oncol Stem Cell Ther 2020 Oct 8. Epub 2020 Oct 8.

Hans Messner Allogeneic Blood and Marrow Transplant Program, Princess Margaret Cancer Center, University of Toronto, Toronto, Ontario, Canada. Electronic address:

Objective/background: Unsuccessful cytogenetic (US) analysis at baseline has been reported to be a poor prognostic feature in patients with acute myeloid leukemia (AML). We conducted this study to examine the prognostic impact of UC/inconclusive cytogenetic analysis on outcomes in patients with AML undergoing allogeneic hematopoietic stem cell transplantation (Allo HSCT).

Methods: We retrospectively analyzed all adults undergoing Allo HSCT for AML from January 2011 to August 2019. Patients with any documented cytogenetic abnormalities were excluded. Baseline characteristics and transplant outcomes were compared between patients with normal cytogenetics and those with UC.

Results: Overall, 243 AML patients (median age, 55 years; 55.1% female) were included. UC were reported in 79 patients, whereas 164 patients had a normal karyotype. The two groups were similar to each other in terms of baseline demographics, treatment received, and transplant related variables. There was no difference between patients with UC and normal cytogenetics in terms of relapse-free survival (66 months vs. 42 months, p = .53) or overall survival (OS; 77 months vs. 76 months, p = .72). Survival parameters remained similar even in subgroup analysis based on NPM1 and FLT3 mutation status. Significant predictors of OS after Allo HSCT in AML patients with UC were increased age at time of Allo HSCT (hazard ratio [HR] = -1.049; 95% confidence interval [CI], 1.005-1.095), favorable (NPM1/FLT3) mutation profile (HR = 0.11; 95% CI, 0.01-0.84), neutrophil engraftment < 17 days, and absence of chronic graft-versus-host disease (HR = 3.27; 95% CI, 1.20-8.60).

Conclusion: Outcomes after Allo HSCT are comparable between AML patients with UC analysis and patients with normal cytogenetics even after stratification based on molecular risk factors. Allogeneic Allo HSCT may mitigate the poor prognosis of UC analysis in patients with AML.
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http://dx.doi.org/10.1016/j.hemonc.2020.09.001DOI Listing
October 2020

Highly biosafe biomimetic stem cell membrane-disguised nanovehicles for cartilage regeneration.

J Mater Chem B 2020 10;8(38):8884-8893

Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.

Cartilage injury is very common and results in considerable pain and osteoarthritis. Owing to its low self-renewal capability, cartilage regeneration is still a great challenge for clinicians. Stem cell therapy has been treated as the most promising treatment for cartilage regeneration in recent decades. However, increasing concerns about the potential biosafety of stem cell products such as immune rejection and neoplastic transformation restrict their further application in clinic. Herein, biomimetic stem cell membrane-disguised nanovehicles without biosafety risks are designed and prepared for cartilage regeneration. In this study, based on the disguise of the natural bone marrow mesenchymal stem cell (BMSC) membrane, Kartogenin (KGN) as a drug for cartilage regeneration was encapsulated into Fe3O4 nanoparticles as the core of biomimetic stem cell nanovehicles. In the core-shell structure of biomimetic stem cell nanovehicles, the fabricated KGN-loaded BMSC membrane-disguised Fe3O4 nanoparticles (KGN-MNPs) showed a stable hybrid structure with a uniform size and morphology in the physiological environments. Moreover, the prepared KGN-MNPs exhibited excellent biocompatibility when disguised with the natural membrane of BMSCs and good biosafety by eliminating the nuclei of BMSCs. In a cartilage defect rat model, compared with pure KGN, the intra-articularly injected KGN-MNPs were capable of regenerating an integrated organized structure with a layer of hyaline-like cartilage in a shorter time due to the retained natural activities of the BMSC membrane. In a word, KGN-MNPs as one kind of our designed biomimetic stem cell nanovehicles enable rapid and high quality cartilage regeneration, and provide a novel and standardized strategy for stem cell therapy in the future.
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http://dx.doi.org/10.1039/d0tb01686aDOI Listing
October 2020

Inter- and Intra-subject Template-Based Multivariate Synchronization Index Using an Adaptive Threshold for SSVEP-Based BCIs.

Front Neurosci 2020 9;14:717. Epub 2020 Sep 9.

Department of Information and Communication Engineering, Tongji University, Shanghai, China.

The steady-state visually evoked potential (SSVEP) has been widely used in brain-computer interfaces (BCIs). Many studies have proved that the Multivariate synchronization index (MSI) is an efficient method for recognizing the frequency components in SSVEP-based BCIs. Despite its success, the recognition accuracy has not been satisfactory because the simplified pre-constructed sine-cosine waves lack abundant features from the real electroencephalogram (EEG) data. Recent advances in addressing this issue have achieved a significant improvement in recognition accuracy by using individual calibration data. In this study, a new extension based on inter- and intra-subject template signals is introduced to improve the performance of the standard MSI method. Through template transfer, inter-subject similarity and variability are employed to enhance the robustness of SSVEP recognition. Additionally, most existed methods for SSVEP recognition utilize a fixed time window (TW) to perform frequency domain analysis, which limits the information transfer rate (ITR) of BCIs. For addressing this problem, a novel adaptive threshold strategy is integrated into the extension of MSI, which uses a dynamic window to extract the temporal features of SSVEPs and recognizes the stimulus frequency based on a pre-set threshold. The pre-set threshold contributes to obtaining an appropriate and shorter signal length for frequency recognition and filtering ignored-invalid trials. The proposed method is evaluated on a 12-class SSVEP dataset recorded from 10 subjects, and the result shows that this achieves higher recognition accuracy and information transfer rate when compared with the CCA, MSI, Multi-set CCA, and Individual Template-based CCA. This paper demonstrates that the proposed method is a promising approach for developing high-speed BCIs.
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http://dx.doi.org/10.3389/fnins.2020.00717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509063PMC
September 2020

miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells.

Mol Cell Biochem 2021 Jan 30;476(1):425-433. Epub 2020 Sep 30.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.

Skeletal muscle satellite cells (SMSCs), also known as a multipotential stem cell population, play a crucial role during muscle growth and regeneration. In recent years, numerous miRNAs have been associated with the proliferation and differentiation of SMSCs in a number of mammalian species; however, the regulatory mechanisms of miR-194-5p in rabbit SMSCs still remain scarce. In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro culture. Taken together, these data demonstrated that miR-194-5p negatively regulates the proliferation and differentiation of rabbit SMSCs by targeting Mef2c.
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http://dx.doi.org/10.1007/s11010-020-03918-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867548PMC
January 2021

Long Non-Coding RNAs in Brown Adipose Tissue.

Diabetes Metab Syndr Obes 2020 15;13:3193-3204. Epub 2020 Sep 15.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.

Obesity has become a widespread disease that is harmful to human health. Fat homeostasis is essentially maintained by fat accumulation and energy expenditure. Studies on brown adipose tissue (BAT) represent a promising opportunity to identify a pharmaceutical intervention against obesity through increased energy expenditure. Long non-coding RNAs (lncRNAs) were thought to be critical regulators in a variety of biological processes. Recent studies have revealed that lncRNAs, including ones that are BAT-specific, conserved, and located at key protein-coding genes, function in brown adipogenesis, white adipose browning (ie, beige adipogenesis), and brown thermogenesis. In this review, we describe lncRNA properties and highlight functional lncRNAs in these biological processes, with the goal of establishing links between lncRNAs and BAT. Based on the advances of lncRNAs in the regulation of BAT, we discussed the advantages of potential lncRNA-based obesity drugs. Further BAT lncRNA-based drug development may provide new exciting approaches to defend obesity by regulation of fat homeostasis.
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http://dx.doi.org/10.2147/DMSO.S264830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507876PMC
September 2020

Location of the Suture Anchor in Hill-Sachs Lesion Could Influence Glenohumeral Cartilage Quality and Limit Range of Motion After Arthroscopic Bankart Repair and Remplissage.

Am J Sports Med 2020 09 17;48(11):2628-2637. Epub 2020 Aug 17.

Department of Sports Medicine, Huashan Hospital, Shanghai, China.

Background: No study has reported clinical evidence for cartilage change in the glenohumeral joint or the cause of loss in range of motion (ROM) after arthroscopic Bankart repair with remplissage technique (BR).

Purpose: To investigate the postoperative features of glenohumeral joint cartilage, ROM, and anchor placement for remplissage at a minimum of 2 years of follow-up after BR and to analyze the correlations.

Study Design: Case-control study; Level of evidence, 3.

Methods: A total of 21 patients who underwent BR received follow-up for a minimum of 2 years. At both preoperative assessment and final follow-up, passive shoulder ROM, Oxford Shoulder Instability Score, Simple Shoulder Test score, and Single Assessment Numerical Evaluation score were assessed. All patients underwent 3.0-T magnetic resonance imaging (MRI) examination at final follow-up. The clinical outcomes, glenohumeral cartilage or Hill-Sachs lesion-related MRI parameters, and their potential correlations were analyzed.

Results: The mean follow-up was 55.0 months (range, 24-119 months). Compared with preoperative assessment, all functional scores significantly improved ( < .001). At the final follow-up, a significant ROM loss (>15°) of external rotation (ER) at the side (ER) was found in 12 patients, among whom 8 patients had significant ROM loss of ER at 90° of abduction as well. Further, 12 patients with decreased ER had significantly higher signal intensity of cartilage on the anterior, middle, and posterior humeral head (anterior, = .002; middle, < .001; posterior, < .001) than 9 patients with normal ER. The ratio of the width of the remplissage anchor to the diameter of the humeral head (w:d ratio) was significantly greater ( = .031) in the decreased ER group than in the normal ER group. Correlation analysis showed that signal intensity on the posterior humeral head and ER loss (ΔER) had a significantly positive correlation ( = 0.516; = .034), while the w:d ratio and ΔER had a significantly positive correlation ( = 0.519; = .039).

Conclusion: At a minimum of 2 years of follow-up, patients who underwent BR showed significant clinical improvement compared with preoperative assessment, except for limitations in ER. The glenohumeral cartilage degeneration (higher signal intensity) after BR had a significantly positive correlation with the postoperative ER loss, which was found to be associated with a relatively medial placement of the remplissage anchor.
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http://dx.doi.org/10.1177/0363546520945723DOI Listing
September 2020

Evolution and Expression of the Membrane Attack Complex and Perforin Gene Family in the Poaceae.

Int J Mol Sci 2020 Aug 10;21(16). Epub 2020 Aug 10.

State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

Membrane Attack Complex and Perforin (MACPF) proteins play crucial roles in plant development and plant responses to environmental stresses. To date, only four genes have been identified in , and the functions of the gene family members in other plants, especially in important crop plants, such as the Poaceae family, remain largely unknown. In this study, we identified and analyzed 42 genes from six completely sequenced and well annotated species representing the major Poaceae clades. A phylogenetic analysis of genes resolved four groups, characterized by shared motif organizations and gene structures within each group. genes were unevenly distributed along the Poaceae chromosomes. Moreover, segmental duplications and dispersed duplication events may have played significant roles during gene family expansion and functional diversification in the Poaceae. In addition, phylogenomic synteny analysis revealed a high degree of conservation among the Poaceae genes. In particular, Group I, II, and III genes were exposed to strong purifying selection with different evolutionary rates. Temporal and spatial expression analyses suggested that Group III genes were highly expressed relative to the other groups. In addition, most genes were highly expressed in vegetative tissues and up-regulated by several biotic and abiotic stresses. Taken together, these findings provide valuable information for further functional characterization and phenotypic validation of the Poaceae gene family.
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http://dx.doi.org/10.3390/ijms21165736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460961PMC
August 2020