DSTS Mandal's College of Pharmacy
Solapur, Maharashtra | India
Main Specialties: Neuropathology, Pharmacology
Additional Specialties: Screening of drugs for neuroprotective activities
Mr. Shivsharan B. Dhadde is serving as Assistant Professor at DSTS Mandal’s College of Pharmacy, Solapur. He completed his graduation and post graduation degree from Rajiv Gandhi University of Health Sciences, Bangalore. Mr. Dhadde, qualified GATE (PY)-2009 and received several scholarships and awards during his studies. His research interests are Meta-Analysis of pharmacological agents and evaluation of drugs for neurological, cardiovascular and metabolic disorders. He is serving as Editorial Board Member and Reviewer for many reputed journals. Mr. Dhadde has more than 6 years of teaching and research experience. He worked as a resource person in more than six national and international workshops/conferences. Mr. Dhadde has presented scientific posters at four national and six international workshops/conferences. There are 20 Journal articles with a cumulative impact factor of 33.818 to his credit. Mr. Dhadde contributed three book chapters and authored a book entitled “Handbook of Experimental Pharmacology” Studium Press (India) Pvt. Ltd.
Primary Affiliation: DSTS Mandal's College of Pharmacy - Solapur, Maharashtra , India
6PubMed Central Citations
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2018, 88, (4): 1523–1529.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
In the present work, toothpastes were prepared for preventing dental caries and periodontal diseases. Nine formulations (TN1 to TN9) were prepared using neem extract and turmeric powder as active principles. These prepared formulations were evaluated for homogeneity, spreadability, fineness, pH, foaming power, tube extrudability, and stability as per the guidelines of the Bureau of Indian Standards and in-vitro antimicrobial activity. The in-vitro antimicrobial activity studies were performed using standard strains such as Candida albicans(ATCC No. 10231), Streptococcus mutans (MTCC No. 497) and Porphyromonas gingivalis(oral gingival swab). A comparative evaluation of the promising toothpaste formulations against selected marketed toothpastes was also carried out. Among the nine prepared formulations TN1 to TN9 (different concentrations of neem and turmeric powder), formulation TN3 (toothpaste containing 0.5% of neem extract and 0.9% of turmeric powder) has shown promising results (inhibition zone diameters of 23.5, 22.5 and 19.5 mm respectively against C. albicans, S. mutans and P. gingivalis). TN3 emerged as the overall best formulation indicating their potential usefulness in preventing various dental problems.
Biomedicine & Pharmacotherapy, 2017; 77: 52-58.
Biomedicine & Pharmacotherapy
Embelia ribes (ER) has been documented in Ayurveda for treating various diseases, including diabetes mellitus (DM). The present systematic review and meta-analysis evaluated the efficacy and safety of ER and its active bio-marker, embelin and its derivatives in the treatment of DM. Literature search was performed in PubMed/MEDLINE, EMBASE, Scopus, ScienceDirect, Scifinder, and Google Scholar. Using Review Manager, meta-analysis of ER/embelin/derivatives of embelin versus diabetic control was performed with inverse-variance model, providing mean differences (MDs) and 95% confidence intervals (CIs). Heterogeneity was determined by I2 statistic. A total of 13 studies were included in the systematic review and meta-analysis, and were conducted in experimental rats. ER and embelin significantly (P≤0.01) resorted blood glucose (MD, -231.30; CI, -256.79, -205.82; and MD, -154.70; CI, -168.65, -140.74) and glycosylated haemoglobin (MD, -6.36; CI, -8.33, -4.39; and MD,-4.68; CI, -7.76, -1.60), respectively. Meta-analysis findings also reported considerable restoration of insulin, lipid profile, haemodynamic parameters, serum and oxidative stress markers. The derivatives of embelin, 6-bromoembelin and vilangin, also improved diabetic condition. In addition, treatments also ameliorated body weight changes due to diabetes. The present systematic review and meta-analysis supports scientific evidence for the antidiabetic activity of ER/embelin/derivatives of embelin. However, further research is warranted in clinical trials to validate the present findings.
Biomed Pharmacother 2017 May 11;89:1061-1066. Epub 2017 Mar 11.
D.S.T.S. Mandal's College of Pharmacy, Solapur, 413 004, Maharashtra, India.
Present study was designed to evaluate the effect of chromium-d-phenylalanine complex (Cr (d-phe)) on indomethacin-induced inflammatory bowel disease (IBD) in rats. Adult Wistar rats were pretreated with vehicle/Cr (d-phe) (30, 60 and 90?g/kg, p.o.) for 11days. On day 8 and 9, after one h of the above mentioned treatment, indomethacin (7.5mg/kg/day,s.c.) was administered to induce IBD. On day 12, blood samples were collected from animals for lactate dehydrogenase (LDH) estimation and ileum was isolated for macroscopic scoring, biochemical estimation (lipid peroxidation, reduced glutathione and myeloperoxidase activity) and histopathological study. Administration of indomethacin significantly altered the serum LDH, macroscopic and microscopic appearance and biochemical parameters in ileum tissue. Cr (d-phe), at all the tested doses, caused a significant reversal of changes induced by indomethacin. Present study demonstrates the protective effect of Cr (d-phe) against indomethacin-induced IBD in rats. The observed protective effect might be attributed to the antioxidant and anti-inflammatory properties of Cr (d-phe).
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Biomed Pharmacother 2017 Feb 13;86:195-204. Epub 2016 Dec 13.
Department of Pharmacology, D.S.T.S. Mandal's College of Pharmacy, Solapur 413004, Maharashtra, India.
Embelia ribes (ER) has been documented in Ayurveda for treating various diseases, including diabetes mellitus (DM). The present systematic review and meta-analysis evaluated the efficacy and safety of ER and its active bio-marker, embelin and its derivatives in the treatment of DM. Literature search was performed in PubMed/MEDLINE, EMBASE, Scopus, ScienceDirect, Scifinder, and Google Scholar. Using Review Manager, meta-analysis of ER/embelin/derivatives of embelin versus diabetic control was performed with inverse-variance model, providing mean differences (MDs) and 95% confidence intervals (CIs). Heterogeneity was determined by I statistic. A total of 13 studies were included in the systematic review and meta-analysis, and were conducted in experimental rats. ER and embelin significantly (P?0.01) resorted blood glucose (MD, -231.30; CI, -256.79, -205.82; and MD, -154.70; CI, -168.65, -140.74) and glycosylated haemoglobin (MD, -6.36; CI, -8.33, -4.39; and MD,-4.68; CI, -7.76, -1.60), respectively. Meta-analysis findings also reported considerable restoration of insulin, lipid profile, haemodynamic parameters, serum and oxidative stress markers. The derivatives of embelin, 6-bromoembelin and vilangin, also improved diabetic condition. In addition, treatments also ameliorated body weight changes due to diabetes. The present systematic review and meta-analysis supports scientific evidence for the antidiabetic activity of ER/embelin/derivatives of embelin. However, further research is warranted in clinical trials to validate the present findings.
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences.
Embelin,isanaturallyoccurringalkylsubstituted hydroxyl benzoquinone and a major constituent from all the parts of Embelia ribes. It possesses antitumor, anti-inﬂammatory, antioxidant, analgesic activities and also has the ability todecrease testosterone levels.Thepresentstudy was designed to determine the effect of embelin on testosteroneinduced benign prostatic hyperplasia (BPH) in rats. Male Wistar rats were randomly divided into six groups and treated with either embelin (5 and 10 mg/kg), ﬁnasteride (1 mg/kg) or vehicle. After above mentioned treatment, testosterone (3 mg/kg; subcutaneously) was administration for 28 days, to induce BPH. On the day 29, blood samples were collected from the retro-orbital plexus. After blood sample collection, animals were sacriﬁced and prostates were immediately dissected out for weight measurement, biochemical estimations and histopathological studies. Prostatic serum acid phosphatase (PSAP) and prostate-speciﬁc antigen (PSA) were estimated. Administration of testosterone leads to signiﬁcant increase in prosthetic weight, PSAP, PSA and lipid peroxidation levels and decrease in the antioxidant status. Moreover, histopathological abnormalities in the prostatic tissue were also found in these animals. Treatment with both the doses of embelin and ﬁnasteride signiﬁcantly reversed the changes produced by testosterone. The authors conclude that antioxidant and anti-inﬂammatory properties of embelin are responsible for its protection against testosterone-induced BPH.
Pharmacol Rep 2016 Oct 29;68(5):903-12. Epub 2016 Jun 29.
VT's Shivajirao S. Jondhle College of Pharmacy, Asangaon 421601, Maharashtra, India.
Eperisone, an analgesic and centrally acting muscle relaxant has been in use for the treatment of low back pain (LBP). The present systematic review evaluates the efficacy and safety of eperisone in patients with LBP. Cochrane Back and Neck (CBN) Group and Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines were adopted to perform this systematic review. For risk of bias assessment CBN Group and Moga tools were used. Seven (5 randomized controlled trials [RCTs] and 2 uncontrolled studies) studies involving 801 participants were included. Eperisone intervention may be effective in acute LBP patients with less adverse effects (relative risk, 0.25; 95% confidence interval, 0.15-0.41; p<0.0001). Eperisone also improved paraspinal blood flow and was found to have efficacy similar to tizanidine in chronic LBP patients. The included studies in this review are of smaller sample size and short duration to support eperisone use in LBP. However, we recommend well-designed RCTs of high quality with larger sample size and longer follow-up to confirm the clinical benefits of eperisone in the treatment of acute or chronic LBP.
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Phytother Res 2016 May 18;30(5):815-22. Epub 2016 Feb 18.
VT's Shivajirao S. Jondhle College of Pharmacy, Asangaon, 421 601, India.
Sickness behaviour is a coordinated set of adaptive behavioural changes that develop in ill individuals during the course of an infection. It is relevant to understanding depression and some aspects of the suffering that in cancer. Embelin has been reported to possess antiinflammatory, neuroprotective and anxiolytic assets and has been shown to inhibit nuclear factor ?B pathway and cytokine production. The present study was undertaken to investigate the effect of embelin isolated from Embelia ribes Burm in lipopolysaccharide (LPS)-induced sickness behaviour in mice. Adult male Swiss albino mice were pre-treated with embelin (10 and 20?mg/kg, p.o.) or dexamethasone (1?mg/kg, i.p.) for 3?days and then challenged with LPS (400?µg/kg, i.p.). At different time intervals of post-LPS challenge, sickness behaviour was evaluated in the animals by battery of behavioural tests (plus maze, open field, light-dark box, forced swim, social behaviour assessment, sucrose preference and food and water intake). Levels of oxidative stress makers (reduced glutathione and lipid peroxidation) in mice brain were also analysed. LPS induced behavioural alterations, anhedonia and anorexia, in mice. Pre-treatment with embelin attenuated behavioural changes induced by LPS. In addition, embelin prevented anhedonia, anorexia and ameliorated brain oxidative stress markers. The experimental outcomes of the present study demonstrated protective effect of embelin in LPS-induced sickness behaviour in mice. Copyright © 2016 John Wiley & Sons, Ltd.
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Toxicol Mech Methods 2014 Dec 24;24(9):672-8. Epub 2014 Sep 24.
Sree Siddaganga College of Pharmacy , Tumkur, Karnataka , India and.
3-Nitropropionic acid (3-NP) is a fungal toxin that produces Huntington's disease like symptoms in both animals and humans. Piroxicam, a non-selective cyclooxygenase (COX) inhibitor, used as anti-inflammatory agent and also known to decrease free oxygen radical production. In this study, the effect of piroxicam was evaluated against 3-NP-induced brain oxidative stress and behavioral alteration in mice. Adult male Swiss albino mice were injected with vehicle/piroxicam (10 and 20?mg/kg, i.p.) 30?min before 3-NP challenge (15?mg/kg, i.p.) regularly for 14 days. Body weights of the mice were measured on alternative days of the experiment. At the end of the treatment schedule, mice were evaluated for behavioral alterations (movement analysis, locomotor test, beam walking test and hanging wire test) and brain homogenates were used for the estimation of oxidative stress markers (lipid peroxidation, reduced glutathione and catalase). Administration of 3-NP significantly altered the behavioral activities and brain antioxidant status in mice. Piroxicam, at both the tested doses, caused a significant reversal of 3-NP-induced behavioral alterations and oxidative stress in mice. These findings suggest piroxicam protects the mice against 3-NP-induced brain oxidative stress and behavioral alteration. The antioxidant properties of piroxicam may be responsible for the observed beneficial actions.
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Drug and chemical toxicology 36 (4), 466-473
Drug and chemical toxicology
Oxidative stress (OS) and nitric oxide mechanisms have been recently proposed in 3-nitropropionic acid (3-NP)-induced neurotoxicity. The compounds, having antioxidant, antiinflammatory and estrogenic effects, have been suggested for neuroprotection in different experimental models. Calendula officinalis Linn. flower extract (COE) is known for its potent antioxidant, anti-inflammatory, estrogenic and neuroprotective activities. Hence, the present study was designed to evaluate the neuroprotective effect of COE on 3-NP-induced neurotoxicity in rats by observing behavioral changes, OS and striatal damage in rat brain. Adult female Wistar rats were pretreated with vehicle or COE (100 and 200 mg/kg) for 7 days, followed by cotreatment with 3-NP (15 mg/kg, intraperitoneally) for the next 7 days. At the end of the treatment schedule, rats were evaluated for alterations in sensory motor functions and short-term memory. Animals were sacrificed and brain homogenates were used for the estimation of lipid peroxidation (LPO), glutathione, total thiols, glutathione S-transferase, catalase and nitrite. A set of brain slices was used for the evaluation of neuronal damage in the striatal region of the brain. 3-NP caused significant alterations in animal behavior, oxidative defense system evidenced by raised levels of LPO and nitrite concentration, and depletion of antioxidant levels. It also produced a loss of neuronal cells in the striatal region. Treatment with COE significantly attenuated behavioral alterations, oxidative damage and striatal neuronal loss in 3-NP-treated animals. The present study shows that COE is protective against 3-NP-induced neurotoxicity in rats. The antioxidant, anti-inflammatory and estrogenic properties of COE may be responsible for its neuroprotective action.
Journal of ethnopharmacology 140 (2), 247-254
Journal of ethnopharmacology
Ethnopharmacological relevance: In the traditional Indian and Thai system of medicine, Mimusops elengi Linn., flower is used as brain tonic and to calm anxiety and panic attacks.
Aim of the study: The present study was designed to investigate the neuroprotective effect of hydroalcoholic extract of Mimusops elengi (ME) against cerebral ischemic reperfusion injury in rats.
Materials and methods: Male rats were pretreated with ME (100 and 200 mg/kg) for seven days and focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) method. After 60 min of MCAO and 24 h of reperfusion, a battery of behavioral tests assessed the extent of neurological deficits. Infarct volume and brain edema were measured in TTC stained brain sections and the extent of blood brain barrier (BBB) disruption was observed by Evan’s blue extravasation. Oxidative and nitrative stress parameters were estimated in the brain homogenates. Further, simultaneous quantification of five polyphenolic biomarkers were done using HPLC.
Results: Pretreatment with ME at doses of 100 and 200 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema and extent of BBB disruption induced by ischemia reperfusion injury. It also prevented the alteration in the antioxidant status and reduced the nitrite levels when compared to ischemic animals. Further, HPLC studies revealed that ME contains five bioactive polyphenolic compounds.
Conclusions: These results clearly indicate the neuroprotective effect of ME against stroke like injury. The observed protective effect might be attributed to the polyphenolic compounds and their antioxidant and anti-inflammatory property.
Toxicology mechanisms and methods 22 (9), 674-678
Toxicology mechanisms and methods
N-methyl-d-aspartate (NMDA) antagonists and γ-aminobutyric acid (GABA) agonists are proven protective in various animal models of ischemic brain damage. Tramadol, a centrally acting opioid analgesic reportedly possesses NMDA antagonistic and GABA agonistic properties, with additional ion channel blocking activity. The aim of the present study was to evaluate the possible neuroprotective effect of tramadol hydrochloride in a rat model of transient forebrain ischemia. Male Wistar rats were pretreated with tramadol hydrochloride at doses of 10 and 20 mg/kg b.w. intraperitoneally for 4 days and were subjected to 30 min occlusion of bilateral common carotid arteries followed by reperfusion for 24 h. Impairment in sensorimotor functions was evaluated by beam walking task, spontaneous locomotor activity and hanging wire test. Animals were sacrificed and the brain homogenates were used for estimating the levels of lipid peroxidation, a marker for extent of oxidative stress. Ischemic rats exhibited a significant decrease in locomotion, grip strength and increase in beam walking latency. Tramadol attenuated the post ischemic motor impairment evidenced by improvement in the performance in sensorimotor tests. The extent of lipid peroxidation was significantly (p < 0.001) reduced by tramadol pretreatment which was higher in ischemic control. This study demonstrates the neuroprotective effect of tramadol against transient forebrain ischemia in rats.
Journal of Environmental Pathology, Toxicology and Oncology 31 (3)
Journal of Environmental Pathology, Toxicology and Oncology
ABSTRACT: Monosodium glutamate (MSG) is commonly used as a flavor enhancer in many countries. However, overconsumption of MSG has been reported to produce detrimental effects on several organs. It mainly affects the normal physiology and function of the brain and causes severe oxidative stress. Mimusops elengi Linn. traditionally is used in many countries as a brain tonic and to calm anxiety and panic attacks. The effect of standardized hydroalcoholic extract of M. elengi flowers (ME) was evaluated against MSG-induced oxidative stress and excitotoxicity in Wistar rats. Excitotoxicity was induced by intraperitoneal administration of MSG (2 g/kg) for 7 days, and ME (100 and 200 mg/kg) was administered for 3 days before and for 7 days with administration of MSG. Animals were evaluated for locomotor activity, and brain homogenates were estimated for the levels of antioxidants and nitrite. In animals treated with MSG, pretreatment with ME improved ambulatory behavior, reduced lipid peroxidation and nitrite levels, and restored the enzymatic and nonenzymatic antioxidant (glutathione, total thiols, glutathione-S-transferase and catalase) status to near-normal levels; these were altered in the MSG control animals. Altogether, this investigation demonstrates the neuroprotective effect of ME against excitotoxicity and oxidative stress induced by MSG, and the observed protective effect might be attributed to the potential antioxidant property of ME.
Indian journal of pharmacology 43 (6), 699
Indian journal of pharmacology
Objective: The aim of the present study was to evaluate the sedative and antiepileptic activities of ethanolic extract of Anthocephalus cadamba (ACE) bark in various experimental animal models.
Materials and Methods: ACE was tested at three doses viz. 100, 200 and 400 mg/kg p.o. We used ketamine-induced sleeping time model to test the sedative property of the extract where, onset and duration of sleep were observed. A paradigm of anticonvulsant models (pentylenetetrazole, isoniazid and maximal electroshock-induced seizures) were used to evaluate its protective effect against absence and generalized types of seizures. Onset of clonic convulsions, tonic extension and time of death were observed in PTZ and INH-induced seizure models. In MES model, duration of tonic hind leg extension and onset of stupor were observed.
Results: ACE showed significant increase in ketamine induced sleeping time. It also exhibited significant increase (P<0.05, 0.01 and 0.001) in latency to clonic convulsion, tonic extension and time of death in PTZ and INH models at all tested doses, whereas in the MES model, the lower dose was found to be effective when compared with the higher doses (200 and 400 mg/kg, p.o.).
Conclusion: The results of the present investigation demonstrated that ACE possesses sedative and antiepileptic activities.
Neurotoxicity research 20 (4), 379-386
Embelia ribes is being used in Indian traditional herbal medicine for the treatment of mental disorders and as brain tonic. The present study was designed to investigate the protective effects of embelin from E. ribes on global ischemia/reperfusion-induced brain injury in rats. Transient global ischemia was induced by occluding bilateral common carotid arteries for 30 min followed by 24-h reperfusion. Neurological functions were measured using sensorimotor tests. Ischemia/reperfusion-induced neuronal injury was assessed by cerebral infarct area, biochemical and histopathological examination. Pretreatment of embelin (25 and 50 mg/kg, p.o.) significantly increased locomotor activity and hanging latency time and decreased beam walking latency when compared with ischemic control. The treatment also reduced significantly the lipid peroxidation and increased the total thiol content and glutathione-S-transferase activity in brain homogenates. The decreased cerebral infarction area in embelintreated groups and histopathological observations confirmed the above findings. These observations suggested that embelin is a neuroprotective agent and may prove to be useful adjunct in the treatment of stroke.
Biomedicine & Preventive Nutrition 1 (4), 273-281
Biomedicine & Preventive Nutrition
Indian Journal of Clinical Biochemistry 28 (3), 292-298
Indian Journal of Clinical Biochemistry