Shiv K. Sharma, Ph.D. - University of Miami - Lecturer/Post-doc

Shiv K. Sharma

Ph.D.

University of Miami

Lecturer/Post-doc

Miami, FL | United States

Additional Specialties: Surface Science

ORCID logohttps://orcid.org/0000-0001-8223-7789

Shiv K. Sharma, Ph.D. - University of Miami - Lecturer/Post-doc

Shiv K. Sharma

Ph.D.

Introduction

Shiv K Sharma is a postdoc and a lecturer of Chemistry at the University of Miami. He received his B.Sc. (2008) and M.Sc. (2012) in Chemistry from Tribhuvan University, Kathmandu. He completed his Ph.D. from the University of Miami in 2018. He works in Surface Chemistry of enzymes, incorporates enzymes with nanoparticles and applies in Biosensing.

Primary Affiliation: University of Miami - Miami, FL , United States

Additional Specialties:

Research Interests:

Education

Aug 2014
University of Miami
Ph.D.
Chemistry

Publications

11Publications

34Reads

5Profile Views

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Molecules 2019, 24(18), 3275

Molecules, MDPI

Nanoparticles have been conjugated to biological systems for numerous applications such as self-assembly, sensing, imaging, and therapy. Development of more reliable and robust biosensors that exhibit high response rate, increased detection limit, and enhanced useful lifetime is in high demand. We have developed a sensing platform by the conjugation of β-galactosidase, a crucial enzyme, with lab-synthesized gel-like carbon dots (CDs) which have high luminescence, photostability, and easy surface functionalization. We found that the conjugated enzyme exhibited higher stability towards temperature and pH changes in comparison to the native enzyme. This enriched property of the enzyme was distinctly used to develop a stable, reliable, robust biosensor. The detection limit of the biosensor was found to be 2.9 × 10−4 M, whereas its sensitivity was 0.81 µA·mmol−1·cm−2. Further, we used the Langmuir monolayer technique to understand the surface properties of the conjugated enzyme. It was found that the conjugate was highly stable at the air/subphase interface which additionally reinforces the suitability of the use of the conjugated enzyme for the biosensing applications

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September 2019

Impact Factor 3.060

Conformation change of α-synuclein(61-95) at the air-water interface and quantitative measurement of the tilt angle of the axis of its α-helix by multiple angle incidence resolution spectroscopy.

Colloids Surf B Biointerfaces 2019 Jul 29;183:110401. Epub 2019 Jul 29.

1301 Memorial Drive, Department of Chemistry, University of Miami, Coral Gables, FL, 33146, USA. Electronic address:

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http://dx.doi.org/10.1016/j.colsurfb.2019.110401DOI Listing
July 2019
1 Read
4.152 Impact Factor

Carbon Dots: Diverse Preparation, Application, and Perspective in Surface Chemistry.

Langmuir 2019 Jul 3;35(28):9115-9132. Epub 2019 Jul 3.

Department of Chemistry , University of Miami , Coral Gables , Florida 33146 , United States.

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http://dx.doi.org/10.1021/acs.langmuir.9b00595DOI Listing
July 2019
4.457 Impact Factor

Carbon Dots: Diverse Preparation, Application, and Perspective in Surface Chemistry

Langmuir 2019, 35, 9115−9132

Langmuir

Carbon dots (CDs) are a novel class of nanoparticles with excellent properties. The development of CDs involves versatile synthesis, characterization, and various applications. However, the importance of surface chemistry of CDs, especially in applications, is often underestimated. In fact, the study of the surface chemistry of CDs is of great significance in the explanation of the unique properties of CDs as well as the pursuit of potential applications. In this feature article, we do not only introduce the development of CDs in our group but also highlight their applications where surface chemistry plays a critical role.

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June 2019

Close-Packed Langmuir Monolayers of Saccharide-Based Carbon Dots at the Air-Subphase Interface.

Langmuir 2019 May 10;35(20):6708-6718. Epub 2019 May 10.

Department of Chemistry , University of Miami , 1301 Memorial Drive , Coral Gables , Florida 33146 , United States.

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http://pubs.acs.org/doi/10.1021/acs.langmuir.9b00920
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http://dx.doi.org/10.1021/acs.langmuir.9b00920DOI Listing
May 2019
4 Reads
4.457 Impact Factor

Interfacial Behavior of Fumonisin B1 Toxin and Its Degradation on the Membrane.

Langmuir 2019 Feb 6;35(7):2814-2820. Epub 2019 Feb 6.

Department of Chemistry , University of Miami , 1301 Memorial Drive , Coral Gables , Florida 33146 , United States.

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http://dx.doi.org/10.1021/acs.langmuir.8b03505DOI Listing
February 2019
2 Reads
4.457 Impact Factor

Surface Chemistry and Spectroscopic Study of a Cholera Toxin B Langmuir Monolayer.

Langmuir 2018 02 5;34(7):2557-2564. Epub 2018 Feb 5.

Department of Chemistry, University of Miami , 1301 Memorial Drive, Coral Gables, Florida 33146, United States.

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http://dx.doi.org/10.1021/acs.langmuir.7b04252DOI Listing
February 2018
5 Reads
4.457 Impact Factor

Biosensors based on β-galactosidase enzyme: Recent advances and perspectives.

Anal Biochem 2017 10 20;535:1-11. Epub 2017 Jul 20.

Department of Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, Florida 33146, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S00032697173031
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http://dx.doi.org/10.1016/j.ab.2017.07.019DOI Listing
October 2017
1 Read
2.219 Impact Factor

Gel-like Carbon Dots: Characterization and their Potential Applications.

Chemphyschem 2017 Apr 23;18(8):890-897. Epub 2017 Feb 23.

Department of Chemistry, University of Miami, Coral Gables, Florida, 33146, USA.

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http://dx.doi.org/10.1002/cphc.201700038DOI Listing
April 2017
11 Reads
3.419 Impact Factor

Polymers in Carbon Dots: A Review.

Polymers (Basel) 2017 Feb 16;9(2). Epub 2017 Feb 16.

Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA.

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http://dx.doi.org/10.3390/polym9020067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432044PMC
February 2017
2.505 Impact Factor

β-Galactosidase Langmuir Monolayer at Air/X-gal Subphase Interface.

J Phys Chem B 2016 12 23;120(48):12279-12286. Epub 2016 Nov 23.

Department of Chemistry, University of Miami , 1301 Memorial Drive, Coral Gables, Florida 33146, United States.

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http://dx.doi.org/10.1021/acs.jpcb.6b09020DOI Listing
December 2016
10 Reads

Top co-authors

Roger M Leblanc
Roger M Leblanc

University of Miami

9
Yiqun Zhou
Yiqun Zhou

Affiliated Hospital of Nantong University

4
Shanghao Li
Shanghao Li

University of Miami

3
Charles C Chusuei
Charles C Chusuei

James Madison University

2
Keenan J Mintz
Keenan J Mintz

UNITED STATES

2
Alexandra Desserre
Alexandra Desserre

CESI Engineering School

1
Zhili Peng
Zhili Peng

University of Miami

1
Osamu Shibata
Osamu Shibata

Graduate School of Pharmaceutical Sciences

1