Shiv K Sharma is a postdoc and a lecturer of Chemistry at the University of Miami. He received his B.Sc. (2008) and M.Sc. (2012) in Chemistry from Tribhuvan University, Kathmandu. He completed his Ph.D. from the University of Miami in 2018. He works in Surface Chemistry of enzymes, incorporates enzymes with nanoparticles and applies in Biosensing.
Primary Affiliation: University of Miami - Miami, FL , United States
Nanoparticles have been conjugated to biological systems for numerous applications such as self-assembly, sensing, imaging, and therapy. Development of more reliable and robust biosensors that exhibit high response rate, increased detection limit, and enhanced useful lifetime is in high demand. We have developed a sensing platform by the conjugation of β-galactosidase, a crucial enzyme, with lab-synthesized gel-like carbon dots (CDs) which have high luminescence, photostability, and easy surface functionalization. We found that the conjugated enzyme exhibited higher stability towards temperature and pH changes in comparison to the native enzyme. This enriched property of the enzyme was distinctly used to develop a stable, reliable, robust biosensor. The detection limit of the biosensor was found to be 2.9 × 10−4 M, whereas its sensitivity was 0.81 µA·mmol−1·cm−2. Further, we used the Langmuir monolayer technique to understand the surface properties of the conjugated enzyme. It was found that the conjugate was highly stable at the air/subphase interface which additionally reinforces the suitability of the use of the conjugated enzyme for the biosensing applications
Carbon dots (CDs) are a novel class of nanoparticles with
excellent properties. The development of CDs involves versatile synthesis,
characterization, and various applications. However, the importance of
surface chemistry of CDs, especially in applications, is often underestimated. In fact, the study of the surface chemistry of CDs is of great
significance in the explanation of the unique properties of CDs as well as
the pursuit of potential applications. In this feature article, we do not only
introduce the development of CDs in our group but also highlight their
applications where surface chemistry plays a critical role.
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