Publications by authors named "Shiquan Wang"

99 Publications

Genome-wide association study-based identification genes influencing agronomic traits in rice (Oryza sativa L.).

Genomics 2021 Mar 9;113(3):1396-1406. Epub 2021 Mar 9.

College of Agronomy, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, China; Rice Research Institute of Sichuan Agricultural University, Chengdu, China. Electronic address:

Rice is one of the most important cereal crops, providing the daily dietary intake for approximately 50% of the global human population. Here, we re-sequenced 259 rice accessions, generating 1371.65 Gb of raw data. Furthermore, we performed genome-wide association studies (GWAS) on 13 agronomic traits using 2.8 million single nucleotide polymorphisms (SNPs) characterized in 259 rice accessions. Phenotypic data and best linear unbiased prediction (BLUP) values of each of the 13 traits over two years of each trait were used for the GWAS. The results showed that 816 SNP signals were significantly associated with the 13 agronomic traits. Then we detected candidate genes related to target traits within 200 kb upstream and downstream of the associated SNP loci, based on linkage disequilibrium (LD) blocks in the whole rice genome. These candidate genes were further identified through haplotype block constructions. This comprehensive study provides a timely and important genomic resource for breeding high yielding rice cultivars.
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http://dx.doi.org/10.1016/j.ygeno.2021.03.016DOI Listing
March 2021

An L-type lectin receptor-like kinase promotes starch accumulation during rice pollen maturation.

Development 2021 Mar 21;148(6). Epub 2021 Mar 21.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China

Starch accumulation is key for the maturity of rice pollen grains; however, the regulatory mechanism underlying this process remains unknown. Here, we have isolated a male-sterile rice mutant, (), which produces nonviable pollen grains with defective starch accumulation. Functional analysis revealed that encodes an active L-type lectin receptor-like kinase (L-LecRLK). AP1 is localized to the plasma membrane and its transcript is highly accumulated in pollen during the starch synthesis phase. RNA-seq and phosphoproteomic analysis revealed that the expression/phosphorylation levels of numerous genes/proteins involved in starch and sucrose metabolism pathway were significantly altered in the mutant pollen, including a known rice UDP-glucose pyrophosphorylase (OsUGP2). We further found that AP1 physically interacts with OsUGP2 to elevate its enzymatic activity, likely through targeted phosphorylation. These findings revealed a novel role of L-LecRLK in controlling pollen maturity via modulating sucrose and starch metabolism.
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http://dx.doi.org/10.1242/dev.196378DOI Listing
March 2021

Identification of rice (Oryza sativa L.) genes involved in sheath blight resistance via a genome-wide association study.

Plant Biotechnol J 2021 Feb 18. Epub 2021 Feb 18.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, China.

Rice sheath blight (RSB) is an economically significant disease affecting rice yield worldwide. Genetic resistance to RSB is associated with multiple minor genes, with each providing a minor phenotypic effect, but the underlying dominant resistance genes remain unknown. A genome-wide association study (GWAS) of 259 diverse rice varieties, with genotypes based on a single nucleotide polymorphism (SNP) and haplotype, was conducted to assess their sheath blight reactions at three developmental stages (seedlings, tillering and booting). A total of 653 genes were correlated with sheath blight resistance, of which the disease resistance protein RPM1 (OsRSR1) and protein kinase domain-containing protein (OsRLCK5) were validated by overexpression and knockdown assays. We further found that the coiled-coil (CC) domain of OsRSR1 (OsRSR1-CC) and full-length OsRLCK5 interacted with serine hydroxymethyltransferase 1 (OsSHM1) and glutaredoxin (OsGRX20), respectively. It was found that OsSHM1, which has a role in the reactive oxygen species (ROS) burst, and OsGRX20 enhanced the antioxidation ability of plants. A regulation model of the new RSB resistance though the glutathione (GSH)-ascorbic acid (AsA) antioxidant system was therefore revealed. These results enhance our understanding of RSB resistance mechanisms and provide better gene resources for the breeding of disease resistance in rice.
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http://dx.doi.org/10.1111/pbi.13569DOI Listing
February 2021

Rosmarinic acid protects rats against post-stroke depression after transient focal cerebral ischemic injury through enhancing antioxidant response.

Brain Res 2021 Apr 4;1757:147336. Epub 2021 Feb 4.

Department of Anesthesiology, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China. Electronic address:

Rosmarinic acid (RA), a natural polyphenol, possesses potent antioxidant and anti-inflammatory activities. To evaluate the ability of RA to cure ischemic stroke and post-stroke depression (PSD), rats were treated with various doses of RA after cerebral ischemia. Neurological deficits and infarct volume of the brain were measured. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were examined at different time points. In addition, a forced swimming test and sucrose preference test were performed to detect the anti-depressive effects of RA. Our results revealed RA administration significantly alleviated neurological deficits and reduced infarct volumes. RA attenuated the decrease of SOD, CAT activities and GSH levels in the ischemic penumbra of the brain. Most importantly, RA treatment alleviated the depression behaviors. Increased expression of Nrf2 was also induced by RA, while down regulation Nrf2 by Nrf2-short-hairpin RNA sequences reversed the increasing activity of SOD and CAT induced by RA, as well as the protection against PSD. The present study indicates that RA exerts a potent neuroprotective effect against stroke and PSD, which could be a promising therapeutic intervention for stroke.
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http://dx.doi.org/10.1016/j.brainres.2021.147336DOI Listing
April 2021

Apigenin-7---D-(-6"--coumaroyl)-glucopyranoside treatment elicits a neuroprotective effect through GSK-3β phosphorylation-mediated Nrf2 activation.

Aging (Albany NY) 2020 11 18;12(23):23872-23888. Epub 2020 Nov 18.

Department of Pharmacy, Xijing Hospital, The Fourth Military Medical School, Xi'an 710032, Shaanxi, China.

The current study was designed to seek the role of the glycogen synthase kinase-3β (GSK-β)-regulated NF-E2-related factor 2 (Nrf2) pathway in the antioxidant effect induced by Apigenin-7-O-β-D-(-6"-p-coumaroyl)-glucopyranoside (APG). Rat primary cultured cortical neurons were challenged by oxygen and glucose deprivation/reoxygenation (OGD/R) and then treated with APG. Cell viability, phosphorylation of GSK-β at Ser9 and nuclear expression of Nrf2 were measured. Male Sprague Dawley rats challenged by 2-h middle cerebral artery occlusion were treated with 50 mg/kg APG, and the neurological score, infarct volume, phosphorylation of GSK-3β and nuclear expression of Nrf2 were analyzed. The neuroprotective effect of APG and the expression levels of antioxidant enzymes and oxidative products were also examined in the presence and absence of Nrf2-siRNA and PI3K inhibitors. APG reduced the apoptotic proportion, attenuated LDH release and increased cell viability, and APG improved neurological scores and reduced infarct volume. APG increased GSK-3β phosphorylation and Nrf2 nuclear translocation, while these effects were prevented by PI3K inhibitors or Nrf2-siRNA treatment in both OGD/R cell cultures and ischemic/reperfusion rats. These findings reveal that GSK-3β phosphorylation-mediated Nrf2 activation is involved in the neuroprotective effect of APG.
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http://dx.doi.org/10.18632/aging.104050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762462PMC
November 2020

CacyBP/SIP protein reduces p53 stability by enhancing Mdm2 activity in p53 mutant glioma cells.

Neoplasma 2021 Jan 3;68(1):119-125. Epub 2020 Sep 3.

Institute of Nervous System Diseases, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, China.

Our previous studies have illustrated that CacyBP/SIP (Calcyclin-binding protein or Siah-1-interacting protein) promoted the proliferation of glioma cells. However, the possible mechanism still needs to be clarified. In the current study, we aimed to uncover the potential mechanism of CacyBP/SIP in regulating glioma cell proliferation. We found that CacyBP/SIP decreased the protein level of p53, but not the mRNA level of p53 in p53 mutant U251 cell line, whereas, in p53 wild-type U87 cell line, CacyBP/SIP neither promoted its proliferation nor regulated the changes of p53 protein. Further investigation indicated that CacyBP/SIP interacted with p53 and Mdm2 (Mouse double minute 2) to promote p53 ubiquitination and subsequent proteasome-mediated degradation in U251. Moreover, in the presence of Mdm2, CacyBP/SIP boosted the ubiquitination of p53 in a dose-dependent manner. On the contrary, inhibition of Mdm2 activity significantly increased the stability of p53. Finally, we found that the protein level of CacyBP/SIP and p53 is inversely correlated in p53 mutant human glioma tissues. These observations suggest an underlying mechanism that CacyBP/SIP promotes the degradation of p53 by enhancing Mdm2 E3 ligase activity, which reveals a novel pathway for the regulation of mutant p53 and provides a new therapeutic approach to target the CacyBP/SIP-induced glioma cell proliferation.
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http://dx.doi.org/10.4149/neo_2020_200414N399DOI Listing
January 2021

A New Cable-Less Seismograph with Functions of Real-Time Data Transmitting and High-Precision Differential Self-Positioning.

Sensors (Basel) 2020 Jul 19;20(14). Epub 2020 Jul 19.

Department of Civil Engineering and Institute for Disaster Management and Reconstruction, Sichuan University, Chengdu 610065, China.

This study developed a new cable-less seismograph system, which can transmit seismic data in real-time and automatically perform high-precision differential self-positioning. Combining the ZigBee technology with the high-precision differential positioning module, this new seismograph system utilized the wireless personal area network (WPAN) and real-time kinematic (RTK) technologies to improve its on-site performances and to make the field quality control (QC) and self-positioning possible. With the advantages of low-cost, good scalability, and good compatibility, the proposed new cable-less seismograph system can improve the field working efficiency and data processing capability. It has potential applications in noise seismology and mobile seismic monitoring.
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http://dx.doi.org/10.3390/s20144015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411714PMC
July 2020

Genistein Attenuates Acute Cerebral Ischemic Damage by Inhibiting the NLRP3 Inflammasome in Reproductively Senescent Mice.

Front Aging Neurosci 2020 17;12:153. Epub 2020 Jun 17.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Postmenopausal women have a higher incidence of stroke compared to the age-matched males, and the estrogen was thought to be the main cause of such difference. However, estrogen replacement therapy for the prevention of postmenopausal stroke shows controversial results and is widely disputed because of its serious side effects after chronic administration. Genistein (Gen), a natural phytestrogen with fewer side effects, has a protective effect against cerebral ischemia damage. However, whether Gen could effectively prevent postmenopausal stroke has not been elucidated. In the current study, reproductively senescent mice were treated with Gen (10 mg/kg) for 2 weeks before having transient cerebral ischemia insults. Neurological scores, infarct volumes, and cell apoptosis were evaluated 24 h after reperfusion. The levels of inflammatory factors and nod-like receptor protein 3 (NLRP3) inflammasome-related proteins were also examined. The results showed that Gen treatment reduced infarct volumes, improved neurological scores, attenuated apoptosis, and decreased inflammatory factor release. The expression of NLRP3 inflammasome-related proteins in microglia was downregulated by Gen. However, the overexpression of NLRP3 in microglia abrogated the Gen-induced inhibition of inflammatory factor release and reversed the neuroprotective effect of Gen. Taken together, the results suggest that Gen treatment could attenuate the acute injury induced by cerebral ischemia in reproductively senescent mice the inhibition of the NLRP3 inflammasome in microglia, indicating that Gen could be a candidate drug for the treatment of stroke in postmenopausal women.
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http://dx.doi.org/10.3389/fnagi.2020.00153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311792PMC
June 2020

1-(2-Cyanoethyl)pyrrole enables excellent battery performance at high temperature via the synergistic effect of Lewis base and C[triple bond, length as m-dash]N functional groups.

Chem Commun (Camb) 2020 Jul;56(60):8420-8423

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Ministry of Educational Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China.

The electrolyte of a lithium ion battery is unstable and is easily decomposed at high temperature, which can lead to the degradation of battery performance. To solve this problem, herein a novel electrolyte additive 1-(2-cyanoethyl)pyrrole (CP) has been proposed to improve the electrochemical performance of LiFePO4 batteries at high temperature. The capacity retention of the battery with 1 wt% CP is 76.7%, while that of the battery without the additive is 38.1% after 200 cycles at 60 °C. Theoretical calculation results reveal that the binding energy of CP and PF5/HF is much higher than that of the solvents in the electrolyte. Surface analysis of the electrodes demonstrates that CP can reduce the decomposition of the electrolyte, and restrain the dissolution of transition metals in the electrolyte at high temperature. TEM/XPS results indicate that CP can modify the protective film on the surface of the cathode material and promote the formation of more regular and thinner CEI films. The promotion of the CP additive is of great significance for improving the high temperature performance of lithium ion batteries and is expected to be applied on a large scale.
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http://dx.doi.org/10.1039/d0cc01528hDOI Listing
July 2020

Glycogenolysis Is Crucial for Astrocytic Glycogen Accumulation and Brain Damage after Reperfusion in Ischemic Stroke.

iScience 2020 May 6;23(5):101136. Epub 2020 May 6.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China; Translational Research Institute of Brain and Brain-Like Intelligence & Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai 200081, China. Electronic address:

Astrocytic glycogen is an important energy reserve in the brain and is believed to supply fuel during energy crisis. However, the pattern of glycogen metabolism in ischemic stroke and its potential therapeutic impact on neurological outcomes are still unknown. Here, we found extensive brain glycogen accumulation after reperfusion in ischemic stroke patients and primates. Glycogenolytic dysfunction in astrocytes is responsible for glycogen accumulation, caused by inactivation of the protein kinase A (PKA)-glycogen phosphorylase kinase (PhK)-glycogen phosphorylase (GP) cascade accompanied by the activation of glycogen synthase kinase-3β (GSK3β). Genetic or pharmacological augmentation of astrocytic GP could promote astrocyte and neuron survival and improve neurological behaviors. In addition, we found that insulin exerted a neuroprotective effect, at least in part by rescuing the PKA-PhK-GP cascade to maintain homeostasis of glycogen metabolism during reperfusion. Together, our findings suggest a promising intervention for undesirable outcomes in ischemic stroke.
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http://dx.doi.org/10.1016/j.isci.2020.101136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240195PMC
May 2020

A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing.

Int J Mol Sci 2020 Mar 16;21(6). Epub 2020 Mar 16.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.

Pollen development plays crucial roles in the life cycle of higher plants. Here we characterized a rice mutant with complete male-sterile phenotype, (). exhibited smaller anthers with arrested pollen development, absent Ubisch bodies, necrosis-like tapetal hypertrophy, and smooth anther cuticular surface. Molecular mapping revealed a synonymous mutation in the fourth exon of co-segregated with the mutant phenotype. This mutation disrupts the exon-intron splice junction in , generating aberrant mRNA species and truncated proteins. is highly expressed in the tapetal cells of developing anther, and its protein is co-localized with plasma membrane (PM) and endoplasmic reticulum (ER) signal. encodes an integrin-α FG-GAP repeat-containing protein, which has seven β-sheets and putative Ca-binding motifs and is broadly conserved in terrestrial plants. Our findings therefore provide insights into both the role of integrin-α FG-GAP repeat-containing protein in rice male fertility and the influence of exonic mutation on intronic splice donor site selection.
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http://dx.doi.org/10.3390/ijms21062018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139555PMC
March 2020

Synthesis and Electrochemical Properties of BiMoO/Carbon Anode for Lithium-Ion Battery Application.

Materials (Basel) 2020 Mar 4;13(5). Epub 2020 Mar 4.

Depatment of Chemical and Process Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford GU2 7XH, UK.

High capacity electrode materials are the key for high energy density Li-ion batteries (LIB) to meet the requirement of the increased driving range of electric vehicles. Here we report the synthesis of a novel anode material, BiMoO/palm-carbon composite, via a simple hydrothermal method. The composite shows higher reversible capacity and better cycling performance, compared to pure BiMoO. In 0-3 V, a potential window of 100 mA/g current density, the LIB cells based on BiMoO/palm-carbon composite show retention reversible capacity of 664 mAh·g after 200 cycles. Electrochemical testing and density functional theory calculations are used to study the fundamental mechanism of Li ion incorporation into the materials. These studies confirm that Li ions incorporate into BiMoO via insertion to the interstitial sites in the MoO-layer, and the presence of palm-carbon improves the electronic conductivity, and thus enhanced the performance of the composite materials.
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http://dx.doi.org/10.3390/ma13051132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085012PMC
March 2020

Deciphering of organic matter and nutrient removal and bacterial community in three sludge treatment wetlands under different operating conditions.

J Environ Manage 2020 Apr 22;260:110159. Epub 2020 Jan 22.

College of Environment and Resources, Dalian Minzu University, Dalian, 116600, China.

Sludge treatment wetlands (STWs) can effectively stabilize sludge, but the microbial community structure in this process is not well characterized. The purpose of this study was to investigate the characteristics of organic matter and nutrient removal and bacterial community in sludge treatment wetlands for treating sewage sludge. Three STWs units included unit STW1 with aeration tubes, unit STW2 with aeration tubes and reed planting and unit STW3 with reed planting. The degradation of organic matter and nutrient, sludge dewatering performance and microbial community dynamics in STWs were examined in feeding and resting periods. Our results showed that during the entire process of the experiment, total solids (TS) in STWs increased to 24-31%, volatile solids (VS) in STWs reduced to 43-47%, while the total kjeldahl nitrogen (TKN) and total phosphorous (TP) concentrations in STWs decreased to 25.1-35.5 mg/g d. w and 5.4-6.2 mg/g d. w. However, the removal efficiencies of organic matter and nutrient in STWs in the feeding period were higher than those in the resting period. Meanwhile, unit STW2 has the best removal performance in organic matter and nutrients during the whole experiment. Microbial community analysis using Illumina MiSeq sequencing technology showed that growth of plants in STWs improved bacterial diversity and richness which corresponded to high removal rates of organic matter and nutrient. Besides, principal coordinate analysis (PCoA) showed that the bacterial community composition in STWs obviously altered between the feeding and the resting periods.
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http://dx.doi.org/10.1016/j.jenvman.2020.110159DOI Listing
April 2020

Evaluation of the fate of nutrients, antibiotics, and antibiotic resistance genes in sludge treatment wetlands.

Sci Total Environ 2020 Apr 30;712:136370. Epub 2019 Dec 30.

School of Environment Science & Technology, Dalian University of Technology, Dalian 116024, China.

The aim of this research was to analyze the elimination of nutrients, antibiotics as well as antibiotic resistance genes (ARGs) in different sludge treatment wetlands (STWs) with or without reeds and aeration tubes. Five antibiotics, including oxytetracycline, tetracycline, azithromycin, sulfamethoxazole, and sulfadiazine; five ARGs, including two tetracycline ARGs (tetC and tetA), one macrolide ARGs (ermB), and two sulfonamide ARGs (sul1 and sul2); and one integrase gene (intI1) were determined in the surface and bottom layers of three STWs, respectively. The removal efficiencies of antibiotics in the bottom layer were lower than that in the surface layer, while the elimination efficiencies of ARGs showed opposite trend. Strong correlations were observed among the contents of antibiotics as well as related ARGs, and the abundance of ARGs had a strong correlation with intI1. The results demonstrated that the contents of these pollutants decreased during the resting period in all the STWs, while the wetland had reeds and aeration tubes performed the best.
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http://dx.doi.org/10.1016/j.scitotenv.2019.136370DOI Listing
April 2020

Integrative transcriptome analysis discloses the molecular basis of a heterogeneous fungal phytopathogen complex, Rhizoctonia solani AG-1 subgroups.

Sci Rep 2019 12 23;9(1):19626. Epub 2019 Dec 23.

Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

Rhizoctonia solani is a fungal species complex that causes necrotrophic crop diseases. It comprises several anastomosis groups, some of which include intra-subgroups, such as AG-1 IA and AG-1 IB, exhibiting varying pathogenicity. Owing to its heterozygous and multinucleate features, genomic analyses of R. solani are still challenging, and understanding of its genetic diversity and genic components is limited. In this study, in order to elucidate the molecular basis of this phytopathogen complex, an integrated transcriptome analysis was undertaken for three subgroups of AG-1, i.e. AG-1 IA, AG-1 IB, and AG-1 IC. Sequence variations suggested substantial evolutionary distances within AG-1. Transcript simple sequence repeats showed comparable characteristics among AG-1, but contained polymorphic sites. Intra-subgroup polymorphisms suggested varying genic heterozygosity within AG-1, suggesting their independent evolutionary trajectory. Sequences of pathogenic factors, phytotoxin biosynthesis pathway enzymes, secreted lignocellulosic enzymes, secreted reactive oxygen species detoxification enzymes, apoplastic/cytoplasmic effector candidates, were conserved among those subgroups. d/d ratios of a secretome subset suggested core secreted proteins in AG-1 and distinct evolution of Cys-rich small secreted proteins after differentiation of AG-1 subgroups. Identification of likely pathogenic factors including allergen protein homologues, oxidative phosphorylation and ethylene biosynthesis pathways, and diversification of polysaccharide monooxygenases provides molecular insight into key genomic components that play a role in R. solani pathogenesis.
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http://dx.doi.org/10.1038/s41598-019-55734-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928066PMC
December 2019

To control or to be controlled? Dual roles of CDK2 in DNA damage and DNA damage response.

DNA Repair (Amst) 2020 01 16;85:102702. Epub 2019 Sep 16.

The Research Center for Medical Genomics, Key Laboratory of Medical Cell Biology, Ministry of Education, School of Life Science, China Medical University, Shenyang, Liaoning Province, PR China. Electronic address:

CDK2 (cyclin-dependent kinase 2), a member of the CDK family, has been shown to play a role in many cellular activities including cell cycle progression, apoptosis and senescence. Recently, accumulating evidence indicates that CDK2 is involved in DNA damage and DNA repair response (DDR). When DNA is damaged by internal or external genotoxic stresses, CDK2 activity is required for proper DNA repair in vivo and in vitro, whereas inactivation of CDK2 by siRNA techniques or by inhibitors could result in DNA damage and stimulate DDR. Hence, CDK2 seems to play dual roles in DNA damage and DDR. On one aspect, it is activated and stimulates DDR to repair DNA damage when DNA damage occurs; on the other hand, its inactivation directly leads to DNA damage and evokes DDR. Here, we describe the roles of CDK2 in DNA damage and DDR, and discuss the potential application of CDK2 inhibitors as anti-cancer agents.
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http://dx.doi.org/10.1016/j.dnarep.2019.102702DOI Listing
January 2020

[Regulatory T cells are involved in sevoflurane preconditioning-induced mouse brain protection against cerebral ischemia injury].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Jul;35(7):601-605

Deparment of Emergence, Xijing Hospital, Air Force Medical University, Xi'an 710032, China. *Corresponding author, E-mail:

Objective To determine whether regulatory T cells (Tregs) are involved in sevoflurane preconditioning-induced brain protection against cerebral ischemia/reperfusion injury. Methods C57BL/6 mice were preconditioned with sevoflurane and then subjected to the middle cerebral artery occlusion modeling. The brain infarct volume and neurological score were assessed at 48 hours after cerebral reperfusion. Meanwhile, the proportion of Tregs in the spleen was analyzed by flow cytometry. Then, CD25 neutralizing antibody was administrated by intraperitoneal injection, following with the analysis of cerebral ischemia/reperfusion injury and the proportion of Tregs in the spleen after sevoflurane preconditioning. Results Compared with a control group, sevoflurane preconditioning markedly mitigated the cerebral ischemia/reperfusion injury in the mice including the infarct volume and neurological score. In the meantime, sevoflurane preconditioning significantly increased the proportion of Tregs in the spleen at 48 hours after cerebral reperfusion. Compared with the isotype antibody group, the CD25 neutralizing antibody reversed the increase of Tregs induced by sevoflurane preconditioning at 48 hours after reperfusion, which was also associated with the reversal of sevoflurane preconditioning-induced protectetion against cerebral ischemia/reperfusion injury. Conclusion Tregs are involved in sevoflurane preconditioning-induced cerebral protection against ischemia/reperfusion injury.
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July 2019

Synthesis of Ni/NiO@MIL-101(Cr) Composite as Novel Anode for Lithium-Ion Battery Application.

J Nanosci Nanotechnol 2019 Dec;19(12):8063-8070

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials and Ministry of Education Key Laboratory for Synthesis and Applications of Organic Functional Molecules, Hubei University, Wuhan, 430062, China.

The poor conductivity is one of the prime reasons restricted MOFs to be applied in the lithium-ion battery system. For the sake of ameliorate this issue, the Ni/NiO was well loaded on the surface of Cr-based metal organic frameworks (MIL-101) by solution impregnation and reduction method to form Ni/NiO@MIL-101(Cr) composites. The as-synthesized Ni/NiO@MIL-101(Cr) was characterized by X-ray powder diffractions, X-ray photoelectron spectroscopy, field emission scanning electron microscope and transmission electron microscope techniques. When used as anode for LIBs, the Ni/NiO@MIL-101(Cr) composite exhibited high reversible capacity (891 mAh g after 100 cycles at a current density of 200 mA g) and stable cycle performance, the coulombic efficiency can maintain in the whole cycle above 95.0%. The reasons for that Ni/NiO@MIL-101(Cr) behaved outstanding electrochemical properties were discussed also. The Ni/NiO@MIL-101(Cr) can be used as promising material for lithium-ion battery application.
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http://dx.doi.org/10.1166/jnn.2019.16763DOI Listing
December 2019

Visible light driven photoelectrochemical sensor for chromium(VI) by using BiOI microspheres decorated with metallic bismuth.

Mikrochim Acta 2019 05 11;186(6):345. Epub 2019 May 11.

Department of Environmental Sciences, Zhejiang Provincial Key Laboratory of Watershed Science and Health, Wenzhou Medical University, Wenzhou, 325035, People's Republic of China.

Composites were prepared from BiOI and Bi/BiOI-X (where x can be 1, 2, 3, or 4) by a one-step solvothermal method and used to design a photoelectrochemical (PEC) assay for chromium(VI). The chemical composition and morphology of the materials were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The results of UV-vis DRS (Diffuse reflection spectra) and photoluminescence show the composites to have higher visible light absorption and a lower electron recombination rate compared to BiOI alone. Photogenerated electrons reduce hexavalent chromium to trivalent chromium, and the consumption of electrons cause noticeable enhances of the photocurrent density after the addition of Cr(VI). Thus, the Cr(VI) concentration can be measured by monitoring the increase of photocurrent density. The Bi/BiOI-3 material displays the best performance for detecting Cr(VI). The method has a wide linear range (1 to 230 μM) and a low detection limit of 0.3 μM (at S/N = 3). It is stable, selective, reproducible and was applied to the determination of nitrite in spiked tap water and lake water samples. Graphical abstract Schematic presentation of a electrochemical sensor based on Bi/BiOI for the determination of Cr(VI).
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http://dx.doi.org/10.1007/s00604-019-3463-0DOI Listing
May 2019

Integrated Polypyrrole@Sulfur@Graphene Aerogel 3D Architecture via Advanced Vapor Polymerization for High-Performance Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2019 May 8;11(20):18448-18455. Epub 2019 May 8.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Ministry of Educational Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering , Hubei University , Wuhan 430062 , P. R. China.

Although lithium-sulfur batteries have been regarded as the most promising candidates for next-generation energy storage devices with high specific capacity, their rapid capacity decay, mainly caused by volume expansion and dissolution of polysulfides, has limited their practical applications. Aiming at these issues, herein, we have designed an ideal three-dimensional (3D)-structured polypyrrole@sulfur@graphene aerogel (PPy@S@GA) as an efficient sulfur host via advanced pyrrole vapor polymerization. GA with an interconnected 3D porous structure provides an excellent conductive network for electrons and a channel for ion transfer, as well as a physical barrier or absorber for the polysulfides. In addition, physical confinement and chemical adsorption are further strengthened by the PPy coating layer with polar nitrogen. The electrode with the PPy@S@GA 3D structure delivered a superior initial discharge specific capacity of 1135 mA h g and a capacity of 741 mA h g after 500 cycles at a rate of 0.5 C, with capacity fading as low as 0.031% per cycle, superior to both a sulfur electrode and a S@GA electrode. These results demonstrate that GA as a sulfur host further coated with PPy is a promising cathode for pursuing high-performance Li-S batteries.
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http://dx.doi.org/10.1021/acsami.9b04167DOI Listing
May 2019

Defective Leptotene Chromosome 1 (DLC1) encodes a type-B response regulator and is required for rice meiosis.

Plant J 2019 08 14;99(3):556-570. Epub 2019 May 14.

State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

Meiosis is critical for sexual reproduction and the generation of new allelic variations in most eukaryotes. In this study, we report the isolation of a meiotic gene, DLC1, using a map-based cloning strategy. The dlc1 mutant is sterile in both male and female gametophytes due to an earlier defect in the leptotene chromosome and subsequent abnormalities at later stages. DLC1 is strongly expressed in the pollen mother cells (PMCs) and tapetum and encodes a nucleus-located rice type-B response regulator (RR) with transcriptional activity. Further investigations showed that DLC1 interacts with all five putative rice histidine phosphotransfer proteins (HPs) in yeast and planta cells, suggesting a possible participation of the two-component signalling systems (TCS) in rice meiosis. Our results demonstrated that DLC1 is required for rice meiosis and fertility, providing useful information for the role of TCS in rice meiosis.
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http://dx.doi.org/10.1111/tpj.14344DOI Listing
August 2019

Seasonal dynamics of bacterial communities associated with antibiotic removal and sludge stabilization in three different sludge treatment wetlands.

J Environ Manage 2019 Jun 2;240:231-237. Epub 2019 Apr 2.

College of Environment and Resources, Dalian Minzu University, Dalian, 116600, China.

In this study, antibiotics removal, sludge stabilization and the change in the bacterial community in sludge treatment wetlands (STWs) were investigated in different seasons. Pilot-scale STWs were characterized for sludge stabilization and the fate of antibiotics in surplus sludge applied during different seasons in three different configurations. The three configurations were unit S1 with ventilation, unit S2 with ventilation and reed plantings and unit S3 with reed plantings. The antibiotics used were ciprofloxacin, azithromycin and oxytetracycline and their degradation, degree of sludge stabilization and bacterial community dynamics were monitored. The results showed that the removal of antibiotics and reduction in the amount of organics in the planted units S2 and S3 were higher than those in the unplanted unit S1, especially in summer. The antibiotic removal efficiency in the planted unit S2, which was equipped with aeration tubes, was the highest over the entire test period. Bacterial community was analyzed by IlluminaMiSeq sequencing of the 16SrRNA gene, showed that the presence of plants in STWs enhanced microbial diversity and richness which promote the removal of antibiotics and sludge stabilization. Proteobacteria, Bacteroidetes and Firmicutes were dominant in the bacterial communities, with Thiobacillus, Dechloromonas and Pseudomonas occurring as dominant genera.
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http://dx.doi.org/10.1016/j.jenvman.2019.03.092DOI Listing
June 2019

Fate of antibiotics in three distinct sludge treatment wetlands under different operating conditions.

Sci Total Environ 2019 Jun 11;671:443-451. Epub 2019 Mar 11.

School of Environment Science & Technology, Dalian University of Technology, Dalian 116024, China.

Sludge treatment wetlands (STWs) have recently been used to treat surplus sludge. However, the distribution of antibiotics involved in the process has not been comprehensively investigated. This study aimed to evaluate the fate of two antibiotics, i.e., ciprofloxacin (CIP) and azithromycin (AZM) in STWs during the treatment of surplus sludge. Three pilot-scale STWs units-S1 with aeration tubes, S2 with aeration tubes and reed planting, and S3 with reed planting-were constructed and operated under feeding followed by resting periods. The results showed that antibiotic content in residual sludge decreased over time and unit S2 performed the best in terms of antibiotic removal. Planting reed considerably improved the antibiotic removal performance of the STWs. Biodegradation and absorption resulted in removal of most of the antibiotics in the test units. Less than 2% of the antibiotics was taken up by plants, whereas <5% of the influent antibiotics left the STW units through the drainage discharge. Overall, STW units contributed to effectively decrease CIP and AZM to 41-72% and 49-84%, respectively.
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http://dx.doi.org/10.1016/j.scitotenv.2019.03.147DOI Listing
June 2019

NiN/NF as Bifunctional Catalysts for Both Hydrogen Generation and Urea Decomposition.

ACS Appl Mater Interfaces 2019 Apr 1;11(14):13168-13175. Epub 2019 Apr 1.

Institute for Sustainable Energy/College of Sciences , Shanghai University , Shanghai 200444 , China.

Oxygen evolution reaction (OER) has a high overpotential, which can significantly reduce the energy efficiency in water decomposition. Using urea oxidation reaction (UOR) to replace OER has been a feasible and energy-saving approach because of its lower electrode potential. Furthermore, UOR is also an important process in wastewater treatment. This paper successfully synthesizes a high-performance bifunctional catalyst for urea electrolysis. The catalyst is nickel nitride bead-like nanospheres array supported on Ni foam (NiN/NF). Several characterization methods are used to analyze the catalyst's morphology, structure, and composition as well as catalytic activity/stability, including X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and electrochemical methods (cyclic voltammetry, linear sweep voltammetry, electrochemical impedance spectroscopy, and CAM). A concurrent two-electrode electrolyzer (NiN/NF∥NiN/NF) is constructed and used to validate the catalyst performance, and the results show that the cell achieves 100 mA·cm at 1.42 V, while the cell voltage of Pt/C∥IrO is 1.60 V, indicating that the NiN/NF catalyst is superior to precious metals.
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http://dx.doi.org/10.1021/acsami.8b19052DOI Listing
April 2019

OsSHOC1 and OsPTD1 are essential for crossover formation during rice meiosis.

Plant J 2019 04 12;98(2):315-328. Epub 2019 Feb 12.

State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130, China.

Meiosis is essential for eukaryotic sexual reproduction and plant fertility, and crossovers (COs) are essential for meiosis and the formation of new allelic combinations in gametes. In this study, we report the isolation of a meiotic gene, OsSHOC1, and the identification of its partner, OsPTD1. Osshoc1 was sterile both in male and female gametophytes, and it showed a striking reduction in the number of meiotic COs, indicating that OsSHOC1 was required for normal CO formation. Further investigations showed that OsSHOC1 physically interacted with OsPTD1 and that the latter was also required for normal CO formation and plant fertility. Additionally, the expression profiles of both genes were consistent with their functions. Our results suggest that OsSHOC1 and OsPTD1 are essential for rice fertility and CO formation, possibly by stabilizing the recombinant intermediates during meiosis.
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http://dx.doi.org/10.1111/tpj.14214DOI Listing
April 2019

Synthesis of CuO/g-CN composites, and their application to voltammetric sensing of glucose and dopamine.

Mikrochim Acta 2018 12 10;186(1):10. Epub 2018 Dec 10.

School of Nuclear Technology and Chemistry & Biology, Hubei University of Science and Technology, Xianning, 437100, China.

The preparation of 3 kinds of carbonaceous nanocomposites by hydrothermal treatment and subsequent calcination described. The first comprises a nanomaterial of type CuO/g-CN, with g-CN in mass fractions of 2, 5 and 7 wt%, respectively. The second comprises CuO/porous carbon (5 wt%), and the third comprises CuO/carbon spheres (5 wt%). All of them were employed to modify a glassy carbon electrode (GCE) to obtain electrochemical sensors for glucose and dopamine. The GCE modified with CuO/g-CN (5 wt%) displays the highest electrocatalytic activity towards glucose and dopamine. Figures of merit for sensing glucose (in 0.1 M NaOH solution) include a wide linear range (0.5 μM to 8.5 mM), a detection limit of 0.150 μM, and a sensitivity of 0.274 μA·μM·cm (at a working potential of 0.60 V vs. Ag/AgCl). The respective data for dopamine (in pH 7.0 solution) are linear ranges from 0.2-16.0 μM and 16.0-78.7 μM, a lower detection limit of 60 nM, and an electrochemical sensitivity of 0.834 and 0.331 μA·μM·cm (at a working potential of 0.22 V vs. Ag/AgCl). The good performance of the modified GCE is attributed to the synergetic interactions between CuO and the appropriate fraction of g-CN, and the improvement of conductivity. Graphical abstract Schematic presentation of a electrochemical sensor based on CuO/g-CN for the determination of glucose and dopamine.
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http://dx.doi.org/10.1007/s00604-018-3120-zDOI Listing
December 2018

Neuroprotective Autophagic Flux Induced by Hyperbaric Oxygen Preconditioning is Mediated by Cystatin C.

Neurosci Bull 2019 Apr 5;35(2):336-346. Epub 2018 Dec 5.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, China.

We have previously reported that Cystatin C (CysC) is a pivotal mediator in the neuroprotection induced by hyperbaric oxygen (HBO) preconditioning; however, the underlying mechanism and how CysC changes after stroke are not clear. In the present study, we demonstrated that CysC expression was elevated as early as 3 h after reperfusion, and this was further enhanced by HBO preconditioning. Concurrently, LC3-II and Beclin-1, two positive-markers for autophagy induction, exhibited increases similar to CysC, while knockdown of CysC blocked these elevations. As a marker of autophagy inhibition, p62 was downregulated by HBO preconditioning and this was blocked by CysC knockdown. Besides, the beneficial effects of preserving lysosomal membrane integrity and enhancing autolysosome formation induced by HBO preconditioning were abolished in CysC rats. Furthermore, we demonstrated that exogenous CysC reduced the neurological deficits and infarct volume after brain ischemic injury, while 3-methyladenine partially reversed this neuroprotection. In the present study, we showed that CysC is biochemically and morphologically essential for promoting autophagic flux, and highlighted the translational potential of HBO preconditioning and CysC for stroke treatment.
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http://dx.doi.org/10.1007/s12264-018-0313-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426805PMC
April 2019

Sevoflurane preconditioning induces tolerance to brain ischemia partially via inhibiting thioredoxin-1 nitration.

BMC Anesthesiol 2018 11 17;18(1):171. Epub 2018 Nov 17.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China.

Background: Sevoflurane preconditioning induces brain ischemic tolerance, but the mechanism remains poorly elucidated. Nitration is an important form of post-translational modification in pathological signaling. This study was to investigate the role of thioredoxin-1 (Trx-1) nitration in neuroprotection effect induced by sevoflurane preconditioning in a transient stroke model in rats.

Methods: Adult male Sprague-Dawley rats were preconditioned with 2% sevoflurane or vehicle oxygen exposure, 1 h per day, for 5 consecutive days. At 24 h after the last exposure, rats were subjected to focal brain ischemia induced by middle cerebral artery occlusion (MCAO) for 90 min, followed by 72-h reperfusion. Trx-1 expression and activity, as well as the content of nitrotyrosine at penumbra were detected at 24 h after preconditioning and 2, 8, 24, 72 h after MCAO. Nitrated Trx-1 was examined by immunoprecipitation at 8 h after MCAO. The role of Trx-1 nitration in ischemic tolerance was assessed by administration of nitrated human-Trx-1 prior to MCAO. Neurological scores, brain infarct volumes and TUNEL staining were evaluated at 24 h after reperfusion.

Results: Ischemic stroke decreased Trx-1 activity but not the expression in penumbra tissue. The content of nitrotyrosine was elevated after MCAO. Preconditioning with sevoflurane increased Trx-1 activity and reduced its nitration at 8 h after MCAO in comparison with vehicle preconditioning. The decrement of Trx-1 activity was correlated with its nitration level. Exogenous administration of nitrated human-Trx-1 reversed the brain ischemic tolerance of sevoflurane preconditioning, exacerbating brain infarct volume, neurobehavioral defects and apoptosis, while administration of human-Trx-1 had no effect on the sevoflurane preconditioning-induced neuroprotection.

Conclusion: Ischemic stroke reduces Trx-1 activity via post-translational nitrative modulation in rats. Sevoflurane preconditioning induces brain ischemic tolerance and anti-apoptosis by partially preserving Trx-1 activity via inhibiting nitration.
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http://dx.doi.org/10.1186/s12871-018-0636-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240283PMC
November 2018

GRAIN LENGTH AND AWN 1 negatively regulates grain size in rice.

J Integr Plant Biol 2019 Oct 16;61(10):1036-1042. Epub 2019 Jan 16.

State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu 611130, China.

Grain size is an important factor determining yield in rice. Here, we identified a recessive mutant gene, grain length and awn 1 (gla1), which caused a significant increase in grain length and weight, and was associated with long awns. The gla1 mutation was mapped to a single-nucleotide polymorphism in a gene encoding a cytoplasmically-localized mitogen-activated protein kinase (MAPK) phosphatase. Overexpression of GLA1 caused a decrease in grain length, and the GLA1 protein interacted with OsMAPK6. These results suggest that GLA1 may serve as a negative regulator of the OsMAPKK4-OsMAPK6 cascade, controlling grain size via the dephosphorylation of OsMAPK6.
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http://dx.doi.org/10.1111/jipb.12736DOI Listing
October 2019

TREK-2 Mediates the Neuroprotective Effect of Isoflurane Preconditioning Against Acute Cerebral Ischemia in the Rat.

Rejuvenation Res 2019 Aug 28;22(4):325-334. Epub 2018 Dec 28.

1Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

It is known that preconditional treatment with volatile anesthetics can induce tolerance of the brain to stroke. A previous study demonstrated that the involvement of TREK-1, a two-pore domain K+ channel, in sevoflurane preconditioning induced neuroprotection against focal cerebral ischemia in rats. The present study testified whether TREK-2, another anesthetic-target K+ channel, is also associated with volatile anesthetic-induced neuroprotection, and further explored its potential mechanism. Rats preconditioned with isoflurane were subjected to 1.4vol% isoflurane plus 98% O (1.5 L/min) inhalation for 1 hour daily and continuing for 5 consecutive days. Then, these rats were subjected to middle cerebral artery occlusion (MCAO) as focal cerebral ischemia model. The expression of TWIK-related K+ channel 2 (TREK-2) was analyzed by western blotting and quantitative real-time RT-PCR, and its downstream signaling molecules, protein kinase C (PKC) alpha, extracellular signal-regulated kinase 1/2 (ERK1/2), and pERK1/2 were detected by western blotting also. Subsequently, the expression of TREK-2 was regulated by siRNA transfection in the brain to clarify its role in the neuroprotection of isoflurane preconditioning. Neurological scores, infarction volume, and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining were examined to evaluate the outcomes. The impact of TREK-2 on the expression of its downstream signaling molecules was also examined for preliminary analysis of the possible mechanisms. Isoflurane preconditioning reduced the infarct volume, inhibited the cell apoptosis, and improved the neurological outcome in rats subjected to MCAO. These effects were parallel with the increase in TREK-2 protein and inhibition of the ERK1/2 phosphorylation. The downregulation of TREK-2 through siRNA could significantly attenuate the isoflurane preconditioning-induced neuroprotective effects. Isoflurane preconditioning-induced neuroprotective effects against ischemia-reperfusion injury are associated with the increase in TREK-2 channel activation. These effects depend on the attenuation of PKC alpha and inhibition of ERK1/2 phosphorylation. Results enrich our understanding on the mechanism of two-pore domain K+ channel in preconditioning-induced tolerance to focal cerebral ischemia.
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http://dx.doi.org/10.1089/rej.2017.2039DOI Listing
August 2019