Publications by authors named "Shiqing Liu"

52 Publications

Lung cancer-associated T cell repertoire as potential biomarker for early detection of stage I lung cancer.

Lung Cancer 2021 Dec 28;162:16-22. Epub 2021 Sep 28.

Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, China; Xiangya Lung Cancer Center, Xiangya Hospital, Central South University, Changsha, China; Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, China; National Clinical Research Center for Geriatric Disorders, Changsha, China. Electronic address:

Background: Early detection of lung cancer in asymptomatic patients remains challenging, especially for stage I. Considering the substantial interaction with tumor immunogenicity, we hypothesized that lung cancer-associated TCR (LC-aTCR) may serve as potential biomarker in early detection of stage I lung cancer.

Methods: Individuals who received low-dose computed tomography (LDCT) screening were enrolled in the study. Surgical tissues and peripheral blood specimens were collected and performed with DNA-based T cell repertoire (TCR) sequencing. The motif-based algorithm was used to deconstruct specific lung cancer-associated TCRs (LC-aTCRs).

Results: A total of 146 individuals participating in the real-world LDCT screening project were enrolled in this study, including 52 patients with pathologically-confirmed stage I lung cancer and 94 non-cancer controls. We developed a motif-based algorithm to define 80 LC-aTCRs in the training cohort. Moreover, in the validation cohort, high sensitivity and specificity was showed in stage I lung cancer with 72% and 91% respectively, and the AUC of the ROC curve was 0.91 (95% CI: 0.85 ∼ 0.96).

Conclusion: This work provides inspiration for stage I lung cancer detection by using blood TCR profiling data. The combination of TCR-based assay and routine screening deserves further testing in larger cohorts.
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http://dx.doi.org/10.1016/j.lungcan.2021.09.017DOI Listing
December 2021

E2F3 promotes liver cancer progression under the regulation of circ-PRKAR1B.

Mol Ther Nucleic Acids 2021 Dec 16;26:104-113. Epub 2021 Jul 16.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

Liver cancer is one of the most lethal malignant tumors in the world. The high recurrence and mortality rate make it urgent for scientists and clinicians to find new targets for better treatment of liver cancer. Here, we found that circ-PRKAR1B expression was increased in the paired intrahepatic metastasis sample through high-throughput sequencing. Further experiments also confirmed its high expression both in carcinoma and metastasis when compared to the paired para-carcinoma and the paired carcinoma, respectively. Mechanism study showed that circ-PRKAR1B could promote liver cancer progression through the miR-432-5p/E2F3 pathway, and microRNA-432-5p could directly target the 3' untranslated region (UTR) of E2F3 mRNA to suppress its translation, thereby influencing liver cancer cell invasion and migration capacities. Clinical data obtained by using online databases based on The Cancer Genome Atlas (TCGA) samples and the clinicopathological data of liver cancer patients who underwent surgery in our hospital in the past 2 years also confirmed the significance of circ-PRKAR1B/miR-432-5p/E2F3 signaling in liver cancer progression. Animal experiments also indicated that targeting this newly identified signaling by overexpressing microRNA-432-5p could suppress the progression of liver cancer. Together, our study suggests that circ-PRKAR1B plays an important role in the regulation of liver cancer progression, and targeting this new circ-PRKAR1B/miR-432-5p/E2F3 signaling may help us find new treatment strategies to better suppress liver cancer progression.
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http://dx.doi.org/10.1016/j.omtn.2021.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413671PMC
December 2021

Self-heating phenomenon of piezoelectric elements excited by a tone-burst electric field.

Ultrasonics 2021 Dec 26;117:106562. Epub 2021 Aug 26.

Department of Mathematics and Materials Research Institute, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address:

Tone-burst excitation is often used for ultrasonic transducers of specific operation modes or for overcoming transducer overheating problems associated with continuous wave (CW) excitation. In this study, a theoretical model for the self-heating phenomenon of a piezoelectric disc element is established to estimate the temperature rise induced by a tone-burst electric field. An analytical solution for the temperature rise of the piezoelectric element is obtained by using Laplace transform method. Numerical simulations and experimental measurements are performed to investigate the influence of different excitation parameters on the temperature rise. By comparing the experimental results with the simulation results, the temperature-rise difference between tone-burst and CW excitations is quantified, and the validity of the theoretical model is verified. Furthermore, a multiparameter estimation method is proposed for the heat convection coefficient and dielectric properties under high-field operating conditions. These results are useful in both optimization of heat dissipation performance and characterization of high-power ultrasonic transducers.
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http://dx.doi.org/10.1016/j.ultras.2021.106562DOI Listing
December 2021

Staff's Psychological Well-Being and Coping Mechanisms During COVID-19 Lockdown in Care Homes for Older Adults: A Structural Equation Modeling Analysis.

Res Gerontol Nurs 2021 Jul-Aug;14(4):180-190. Epub 2021 May 19.

The current study investigated the psychological well-being and coping mechanisms of care home staff during the coronavirus disease 2019 (COVID-19) lockdown. Among 147 staff members, 21.8%, 24.5%, and 12.9% reported depression, anxiety, and stress, respectively. The results of structural equation modeling showed that self-efficacy and coping strategies had direct effects on psychological well-being. In the paths from self-perceived caregiving to psychological well-being, self-efficacy and positive coping strategies played mediating roles independently and together, and social support played a mediating role together with coping strategies. Care home staff's psychological well-being was undermined during the COVID-19 lockdown. Interventions aimed at improving staff's self-efficacy, adoption of positive coping strategies, and avoidance of negative coping strategies or considerations of social support along with coping strategies are suggested to decrease exhibited symptoms of depression, anxiety, and stress. [(4), 180-190.].
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http://dx.doi.org/10.3928/19404921-20210325-01DOI Listing
August 2021

Hydrogen-rich saline regulates the polarization and apoptosis of alveolar macrophages and attenuates lung injury via suppression of autophagy in septic rats.

Ann Transl Med 2021 Jun;9(12):974

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: Hydrogen-rich saline (HRS) has a protective effect on sepsis-induced lung injury. However, the underlying mechanisms are still unclear. Polarization and apoptosis of macrophages are essential factors in the pathogenesis of acute lung injury (ALI). Moreover, autophagy is involved in the regulation of both macrophage polarization and apoptosis. Therefore, this study investigated the ability of HRS to attenuate ALI through regulation of the polarization and apoptosis of alveolar macrophages (AMs) during sepsis by modulating autophagy.

Methods: Male Sprague-Dawley (SD) rats were used to prepare the sepsis-induced lung injury animal model. Rat lung tissue was harvested after lipopolysaccharide (LPS) treatment, in the presence or absence of HRS, and the AMs were analyzed for changes in polarization, apoptosis, and autophagy. The rat AM cell line NR8383 was used to examine these processes in vitro using Western blot analysis, flow cytometry, and transmission electron microscopy.

Results: LPS-induced ALI in rats was associated with an increase in autophagy, apoptosis, and M1 polarization but a decrease in M2 polarization in AMs. These effects were reversed by administration of HRS. Inhibition of AM autophagy with 3-methyladenine (3-MA) decreased apoptosis and M1 polarization and increased M2 polarization, paralleling the effects of HRS.

Conclusions: HRS could attenuate ALI in septic rats through regulation of AM polarization and a reduction in apoptosis by suppressing autophagy. This may represent a potential novel therapeutic target for the treatment of ALI caused by sepsis.
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http://dx.doi.org/10.21037/atm-21-2489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267271PMC
June 2021

Epidemiologic characteristics and double-buffered strategy for patients in orthopedic surgery during the novel coronavirus outbreak: Wuhan's experience.

Eur J Med Res 2021 Apr 30;26(1):39. Epub 2021 Apr 30.

Department of Orthopedics, Renmin Hospital of Wuhan University, #238 Jiefang Road, Wuhan, 430060, Hubei, People's Republic of China.

Background: The purpose of this article is to summarize the epidemiologic characteristics and double-buffered strategy for patients in orthopedic surgery during the COVID-19 outbreak in Wuhan, China, based on our own experience in our hospital.

Methods: A retrospective and comparative study was performed to identify all inpatients at our clinic from February 17 to April 20, 2020 (epidemic group), and from February 17 to April 20, 2019 (control group). Epidemiologic characteristics, screening effect, perioperative complications, and nosocomial infection were analyzed.

Results: In the epidemic group, 82 patients were identified, a decrease by 76.0% than the 342 patients in the same period in the 2019. Patients in the epidemic group (54.6 ± 20.2 years) were older than those in the control group (49.6 ± 22.5 years). For the epidemic group, the proportion rates of traumatic factures (69.5%) and low-energy injuries (86.0%) were higher than that in the control group, respectively (35.4% and 37.2%). The preoperative waiting time (7.0 ± 2.6 days) in the epidemic group was longer than that in the control group (4.5 ± 2.1 days). The postoperative complication rate (12.2%) in the epidemic group was higher than that in the control group (3.5%). No nosocomial infection of orthopedic staff and patients with COVID-19 was noted in the epidemic group.

Conclusion: During the COVID-19 outbreak in Wuhan, China, orthopedic inpatients showed unique epidemiological characteristics. The double-buffered strategy could effectively avoid nosocomial infections among medical staff and patients. Doctors should fully evaluate the perioperative risks and complications.
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http://dx.doi.org/10.1186/s40001-021-00510-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085651PMC
April 2021

Loss of androgen receptor promotes HCC invasion and metastasis via activating circ-LNPEP/miR-532-3p/RAB9A signal under hypoxia.

Biochem Biophys Res Commun 2021 06 13;557:26-32. Epub 2021 Apr 13.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China; International Joint Research Center of Minimally Invasive Endoscopic Technology Equipment and Standards, China. Electronic address:

Development of novel targeted therapies remains the priority in hepatocellular carcinoma (HCC) treatments. Early reports have demonstrated that androgen receptor (AR) plays a suppressive role in HCC progression. However, the underlying mechanisms by which AR attenuates HCC development are still elusive, especially under hypoxic conditions. Herein, we demonstrated that AR/circ-LNPEP/miR-532-3p/RAB9A signaling axis was tightly involved in hypoxia-induced cell invasion of HCC cells. AR worked as a transcription factor to reduce circ-LNPEP expression level, which released its sponge potential of miR-532-3p, leading to the downregulation of RAB9A and inhibiting cell invasion of HCC cells. In vitro and in vivo animal model also confirmed that overexpression of circ-LNPEP could reverse the suppressive effect of AR on HCC cell invasion or tumor metastasis. Overall, our study supplements a critical mechanism by which AR suppresses HCC invasion/metastasis under hypoxic conditions, providing compelling rationale to develop novel therapy for better treatments of HCC.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.120DOI Listing
June 2021

Androgen receptor (AR) decreases HCC cells migration and invasion via miR-325/ACP5 signaling.

J Cancer 2021 30;12(7):1915-1925. Epub 2021 Jan 30.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.

Hepatocellular carcinoma (HCC) is the most 5th commonly diagnosed and 2nd most lethal tumor in the world. The obvious gender advantage of HCC indicates that androgen receptor (AR) may play an important role in the tumor occurrence, develop and metastasis of HCC. Here we found that decreased AR could alter miR-325 to increase ACP5 expression in HCC cells, to increase HCC cells migration and invasion capacities. Mechanism dissection revealed that AR could regulate miR-325 expression through transcriptional regulation and miR-325 might directly target the 3'UTR of ACP5-mRNA to suppress its translation. The orthotopic xenografts mouse model with oemiR-325 also validated data. Together, these findings suggest that AR may decrease HCC progression through miR-325/ACP5 signaling and targeting the AR/miR-325/ACP5 signaling may help in the development of the novel therapies to better suppress the HCC progression.
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http://dx.doi.org/10.7150/jca.49200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974538PMC
January 2021

Exosomes derived from miR-126-3p-overexpressing synovial fibroblasts suppress chondrocyte inflammation and cartilage degradation in a rat model of osteoarthritis.

Cell Death Discov 2021 Feb 24;7(1):37. Epub 2021 Feb 24.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.

MicroRNAs (miRNAs) encapsulated within exosomes can serve as essential regulators of intercellular communication and represent promising biomarkers of several aging-associated disorders. However, the relationship between exosomal miRNAs and osteoarthritis (OA)-related chondrocytes and synovial fibroblasts (SFCs) remain to be clarified. Herein, we profiled synovial fluid-derived exosomal miRNAs and explored the effects of exosomal miRNAs derived from SFCs on chondrocyte inflammation, proliferation, and survival, and further assessed their impact on cartilage degeneration in a surgically-induced rat OA model. We identified 19 miRNAs within synovial fluid-derived exosomes that were differentially expressed when comparing OA and control patients. We then employed a microarray-based approach to confirm that exosomal miRNA-126-3p expression was significantly reduced in OA patient-derived synovial fluid exosomes. At a functional level, miRNA-126-3p mimic treatment was sufficient to promote rat chondrocyte migration and proliferation while also suppressing apoptosis and IL-1β, IL-6, and TNF-α expression. SFC-miRNA-126-3p-Exos were able to suppress apoptotic cell death and associated inflammation in chondrocytes. Our in vivo results revealed that rat SFC-derived exosomal miRNA-126-3p was sufficient to suppress the formation of osteophytes, prevent cartilage degeneration, and exert anti-apoptotic and anti-inflammatory effects on articular cartilage. Overall, our findings indicate that SFC exosome-delivered miRNA-126-3p can constrain chondrocyte inflammation and cartilage degeneration. As such, SFC-miRNA-126-3p-Exos may be of therapeutic value for the treatment of patients suffering from OA.
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http://dx.doi.org/10.1038/s41420-021-00418-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904758PMC
February 2021

Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.

PeerJ 2021 8;9:e10470. Epub 2021 Jan 8.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

MicroRNA (miRNA, miR) has been reported to be highly implicated in a wide range of biological processes in lung cancer (LC), and identification of differentially expressed miRNAs between normal and LC samples has been widely used in the discovery of prognostic factors for overall survival (OS) and response to therapy. The present study was designed to develop and evaluate a miRNA-based signature with prognostic value for the OS of lung adenocarcinoma (LUAD), a common histologic subtype of LC. In brief, the miRNA expression profiles and clinicopathological factors of 499 LUAD patients were collected from The Cancer Genome Atlas (TCGA) database. Kaplan-Meier (K-M) survival analysis showed significant correlations between differentially expressed miRNAs and LUAD survival outcomes. Afterward, 1,000 resample LUAD training matrices based on the training set was applied to identify the potential prognostic miRNAs. The least absolute shrinkage and selection operator (LASSO) cox regression analysis was used to constructed a six-miRNA based prognostic signature for LUAD patients. Samples with different risk scores displayed distinct OS in K-M analysis, indicating considerable predictive accuracy of this signature in both training and validation sets. Furthermore, time-dependent receiver operating characteristic (ROC) analysis demonstrated the nomogram achieved higher predictive accuracy than any other clinical variables after incorporating the clinical information (age, sex, stage, and recurrence). In the stratification analysis, the prognostic value of this classifier in LUAD patients was validated to be independent of other clinicopathological variables, such as age, gender, tumor recurrence, and early stage. Gene set annotation analyses were also conducted through the Hallmark gene set and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, indicating target genes of the six miRNAs were positively related to various molecular pathways of cancer, such as hallmark UV response, Wnt signaling pathway and mTOR signaling pathway. In addition, fresh cancer tissue samples and matched adjacent tissue samples from 12 LUAD patients were collected to verify the expression of miR-582's target genes in the model, further revealing the potential relationship between SOX9, RASA1, CEP55, MAP4K4 and LUAD tumorigenesis, and validating the predictive value of the model. Taken together, the present study identified a robust signature for the OS prediction of LUAD patients, which could potentially aid in the individualized selection of therapeutic approaches for LUAD patients.
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http://dx.doi.org/10.7717/peerj.10470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798616PMC
January 2021

Airway Rupture Caused by Double-Lumen Tubes: A Review of 187 Cases.

Anesth Analg 2020 11;131(5):1485-1490

From the Departments of Anesthesiology.

The double-lumen tubes (DLTs) are the most widely used devices to provide perioperative lung isolation. Airway rupture is a rare but life-threatening complication of DLTs. The primary aim of this review was to collect all cases reported in the literature about airway rupture caused by DLTs and to describe the reported possible contributors, diagnosis, treatment, and outcomes of this complication. Another aim of this review was to assess the possible factors associated with mortality after airway rupture by DLTs. A comprehensive literature search for all cases of airway rupture caused by DLTs was performed in the PubMed, EMBASE, Ovid, Wanfang Database, and CNKI. The extracted data included age, sex, height, weight, type of operation, type and size of DLT, site of airway rupture, possible contributors, clinical presentation, diagnosis timing, treatment, and outcome. We included 105 single case reports and 22 case series with a total number of 187 patients. Most of the ruptures were in the trachea (n = 98, 52.4%) and left main bronchus (n = 70, 37.4%). The common possible contributors include use of a stylet, cuff overdistention, multiple attempts to adjust the position of a DLT, difficult intubation, and use of an oversized DLT. Most of the airway ruptures were diagnosed intraoperatively (n = 138, 82.7%). Pneumomediastinum, air leakage, hypoxemia, and subcutaneous emphysema were the common clinical manifestations. Most patients were treated with surgical repair (n = 147, 78.6%). The mortality of the patients with airway rupture by DLTs was 8.8%. Age, sex, site of rupture, diagnosis timing, and method of treatment were not found to be associated with mortality.
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http://dx.doi.org/10.1213/ANE.0000000000004669DOI Listing
November 2020

Knowledge of and attitude toward xenotransplantation among medical students in China: A cross-sectional study.

Xenotransplantation 2021 01 13;28(1):e12654. Epub 2020 Oct 13.

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shengyang, China.

Background: There are great challenges in the psychosocial aspects of xenotransplantation. The aim of this study was to investigate the knowledge of and attitude toward xenotransplantation among medical students in China.

Methods: A questionnaire consisting of 12 questions was distributed to 1000 first-to-fifth grade medical students, with 200 students in each grade. The questionnaire was self-administered and completed anonymously with no information beforehand.

Results: The response rate of the questionnaire was 91.5%. Most students had a good knowledge about xenotransplantation. If the results of xenotransplantation were similar with human donation, the acceptance was 63.9% when both organs were available, 70.3% to shorten the waiting time, and 86.4% to save one's life. As for the risks of xenotransplantation, 46.2%, 46.1%, and 36.9% of the respondents were worried about the risk of disease transmission, immunosuppressants, and other unknown risks, respectively. With regard to the psychosocial impacts, 38.4%, 31.9%, and 52.3% were worried about changing one's nature as a human being, changing one's personality, and discrimination by others, respectively.

Conclusion: Most medical students in China had a favorable attitude toward xenotransplantation. Students in the clinical years had a better knowledge of and more favorable attitude toward xenotransplantation. Their concern about the risks of xenotransplantation resolved slightly with seniority, but their concern about psychosocial impacts did not resolve with seniority.
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http://dx.doi.org/10.1111/xen.12654DOI Listing
January 2021

Chemically modified curcumin (CMC2.24) alleviates osteoarthritis progression by restoring cartilage homeostasis and inhibiting chondrocyte apoptosis via the NF-κB/HIF-2α axis.

J Mol Med (Berl) 2020 10 28;98(10):1479-1491. Epub 2020 Aug 28.

Department of Orthopedics, Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

The disorders of cartilage homeostasis and chondrocyte apoptosis are major events in the pathogenesis of osteoarthritis (OA). Herein, we aim to assess the chondroprotective effect and underlying mechanisms of a novel chemically modified curcumin, CMC2.24, in modulating extracellular matrix (ECM) homeostasis and inhibiting chondrocyte apoptosis. Rats underwent the anterior cruciate ligament transection, and medial menisci resection was treated by intra-articular injection with CMC2.24. In an in vitro study, rat chondrocytes were pretreated with CMC2.24 before stimulation with sodium nitroprusside (SNP). Results from in vivo studies demonstrated that the intra-articular administration of CMC2.24 ameliorated osteoarthritic cartilage destruction by promoting collagen 2a1 production and inhibited cartilage degradation and apoptosis by suppressing hypoxia-inducible factor-2a (Hif-2α), matrix metalloproteinase-3, runt-related transcription factor 2, cleaved caspase-3, and vascular endothelial growth factor and the phosphorylation of IκBα and NF-κB p65. The in vitro results revealed that CMC2.24 exhibited a strong inhibitory effect on SNP-induced chondrocyte catabolism and apoptosis. The SNP-enhanced expression of Hif-2α, a catabolic and apoptotic factor, decreased in a dose-dependent manner after CMC2.24 treatment. CMC2.24 pretreatment effectively inhibited SNP-induced IκBα and NF-κB p65 phosphorylation in rat chondrocytes, whereas pretreatment with the NF-κB antagonist BMS-345541 significantly enhanced the effects of CMC2.24. Overall, these results demonstrated that CMC2.24 attenuates OA progression by modulating ECM homeostasis and chondrocyte apoptosis by suppressing the NF-κB/Hif-2α axis, thus providing a new perspective for therapeutic strategies in OA. KEY MESSAGES: • Intra-articular injection of CMC2.24 ameliorated osteoarthritic cartilage destruction. • CMC2.24 promoted cell viability and decreased SNP-induced apoptotic gene expression. • SNP-induced activation of Hif-2α is inhibited by CMC2.24. • CMC2.24 inhibits NF-κB/Hif-2α axis activation to modulate ECM homeostasis and inhibit chondrocyte apoptosis.
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http://dx.doi.org/10.1007/s00109-020-01972-1DOI Listing
October 2020

Prediction of Double-Lumen Tube Size By Ultrasonography for Asian Women: A Prospective Observational Study.

J Cardiothorac Vasc Anesth 2021 May 31;35(5):1410-1415. Epub 2020 Jul 31.

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China. Electronic address:

Objective: To assess the agreement of the diameter of the cricoid cartilage by computed tomography and ultrasonography and to compare the accuracy of the left double-lumen tubes (DLTs) and right DLTs predicted by ultrasonography for Asian women.

Design: Prospective observational study.

Setting: Academic, tertiary care hospital.

Participants: Fifty female patients intubated with a left DLT and 50 female patients intubated with a right DLT.

Interventions: No intervention.

Measurements And Main Results: A radiologist measured the transverse cricoid diameter by computed tomography (CT), and an independent echographer measured the transverse cricoid diameter using ultrasonography. The size of the DLT was selected based on the cricoid diameter by ultrasonography. The agreement of the transverse cricoid diameter was assessed by computed tomography and ultrasonography. The accuracy of the DLT, the tracheal segment, and the bronchial segment were compared between the left intubation group and right intubation group. There was a good agreement between the transverse cricoid diameter measured by ultrasonography and CT (r = 0.946, p < 0.001). The overall accuracy of the DLTs was similar between the groups (86.0% v 92.0%, p = 0.318). There were no significant differences in the accuracy of the tracheal segment (96.0% v 94.0%; p = 1.000) and the bronchial segment (90.0% v 98.0%, p = 0.056).

Conclusions: The transverse diameter of the cricoid cartilage in most Asian women can be accurately measured by ultrasonography. The size of the DLT for Asian women can be predicted by ultrasonography measurement of the cricoid diameter.
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http://dx.doi.org/10.1053/j.jvca.2020.07.077DOI Listing
May 2021

The Incidence of Tracheal Bronchus in Thoracic Surgery Patients and Its Implication for Lung Isolation: A Retrospective Cohort Study.

J Cardiothorac Vasc Anesth 2020 Nov 26;34(11):3068-3072. Epub 2020 Jun 26.

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China.

Objective: To investigate the incidence of tracheal bronchus (TB) and explore its implication for lung isolation.

Design: Retrospective cohort study.

Setting: Academic, tertiary care hospital.

Participants: The study comprised 7,102 thoracic patients with- one lung ventilation.

Interventions: No intervention.

Measurements And Main Results: Two independent anesthesiologists reviewed the computed tomography images to identify the presence of a TB, and their results were confirmed by a radiologist. The clinical data of patients with a TB were obtained from the electronic medical record. Data regarding the device used to provide lung isolation, preoperative oxygen saturation (SpO), and intraoperative SpO during one- lung ventilation were obtained from the electronic anesthesia record. The incidence of TB was 1.08% (77 of 7,102). The TB arose from the right side of the trachea in all 77 patients, including 70 type Ⅲ TBs and 7 type Ⅱ TBs. Left- and right-sided double-lumen tubes (DLTs) were used in 54 and 23 patients, respectively. For patients with a left-sided DLT, the median SpO and incidence of hypoxemia (SpO <90%) were 97% and 6 of 54 (11.1%), respectively. For patients with a right DLT, the median SpO and incidence of hypoxemia were 95% and 7 of 20 (35.0%), respectively. There were significant differences in the mean SpO and the incidence of hypoxemia between patients intubated with left- and right-sided DLTs (p = 0.014 and p = 0.016, respectively).

Conclusions: Preoperative diagnosis of TB is important when lung isolation is needed. The left-sided DLT can be used for most patients with a TB.
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http://dx.doi.org/10.1053/j.jvca.2020.06.073DOI Listing
November 2020

Age-related Change of the Dimensions of the Cricoid Cartilage in Adults.

Ann Otol Rhinol Laryngol 2021 Feb 10;130(2):153-160. Epub 2020 Jul 10.

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China.

Objectives: To measure the dimensions of the cricoid cartilage in adults and to investigate the age-related change of the dimensions of the cricoid cartilage.

Methods: After performing the multiplanar reconstruction and correcting the slant of the cervical computed tomography scans, the transverse and anteroposterior internal diameters of the inlet (TD-in and APD-in) and outlet (TD-out and APD-out) of the cricoid cartilage were measured, respectively. The angle between the arch and lamina of the cricoid cartilage in the middle sagittal plane was measured. The ratios of transverse to anteroposterior diameter for the inlet (Ratio-in) and outlet (Ratio-out) of the cricoid cartilage were calculated, respectively.

Results: A total of 1200 adults were included in this study, with 600 males and 600 females. The TD-in is the smallest cricoid diameter and the APD-in is the largest cricoid diameter. The mean cricoid diameters and the cricoid angle in males were larger than those in females. The cricoid inlet is oval shaped and the cricoid cartilage is "funnel-shaped" in the middle sagittal plane. The shape of the outlet of the cricoid cartilage varies greatly among individuals. In males, the APD-in and APD-out were negatively correlated with age while the Ratio-in and Ratio-out was positively correlated with age. In females, the APD-out were negatively correlated with age while the Ratio-out was negatively correlated with age.

Conclusions: The dimensions of the cricoid cartilage change as age advances in adult population and the sexual dimorphism of the cricoid outlet occurs after 50 years old.
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http://dx.doi.org/10.1177/0003489420940339DOI Listing
February 2021

Medical Students' Attitudes Toward Deceased Organ Donation in China: A Cross Section Cohort Study.

Transplant Proc 2020 Dec 20;52(10):2890-2894. Epub 2020 Jun 20.

Department of Surgery, Shengjing Hospital of China Medical University, Shengyang, China.

Background: Influenced by traditional culture and lack of knowledge among Chinese, China has a low rate of organ donation after death. The aims of this study were to assess the attitude toward deceased organ donation among medical students in China and to identify the possible factors related with this attitude.

Methods: An anonymous, self-administered questionnaire comprising 15 questions was distributed to 1500 first-to-fifth grade medical students, with 300 students in each grade.

Results: The response rate was 90.9% (n = 1363). The level of knowledge about organ donation and the level of knowledge about the transplantation law were positively related with the seniority (P < .001). The attitude was favorable in 62.7% (n = 855) and against in 37.3% (n = 508) of the respondents. The attitude was related with family discussion about organ donation (P = .002), experience of blood donation (P = .004), willingness to accept a donated deceased organ (P < .001), and the decision of donating the organs of a family member (P < .001). No evidence of relation was detected for age, sex, grade, being an only child, coming from a city or village, knowledge of organ transplantation, knowledge of the transplantation law, and experience of organ donation and transplantation.

Conclusions: Most medical students in China had a favorable attitude toward deceased organ donation. This attitude was positively related with family factors, experience of blood donation, and willingness to accept a donated deceased organ. This attitude did not increase with seniority and a rising level of medical knowledge.
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http://dx.doi.org/10.1016/j.transproceed.2020.02.167DOI Listing
December 2020

Value of ambroxol in the treatment of asthmatic bronchitis.

Pak J Med Sci 2020 Mar-Apr;36(3):501-504

Shufen Su, Department of Endoscopic-Surgery, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Shandong, 250014, China.

Objective: To investigate the clinical effect of ambroxol in the treatment of asthmatic bronchitis.

Methods: One hundred and twenty patients with asthmatic bronchitis who were admitted to our hospital from June 2017 to August 2018 were selected as the research subjects and divided into a control group and an observation group according to random number table method, 60 in each group. The control group was treated with conventional treatment, while the observation group was treated with ambroxol in addition to conventional treatment. The therapeutic effect, disappearance time of symptoms and signs and the recovery of pulmonary function were compared between the two groups.

Results: The total effective rate of the observation group was 96.7%, and that of the control group was 73.3%. The control effect of the observation group was significantly better than that of the control group, showing a significant difference (P<0.05). The disappearance time of symptoms of the observation group was shorter than that of the control group, and the recovery of pulmonary function was better; the differences were statistically significant (P<0.05).

Conclusion: For asthmatic bronchitis patients, addition of ambroxol to conventional treatment can improve the therapeutic effect, shorten the disappearance time of clinical signs and symptoms, and promote the recovery of patients, which is worth clinical application.
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http://dx.doi.org/10.12669/pjms.36.3.1607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150415PMC
April 2020

Berberine-mediated up-regulation of surfactant protein D facilitates cartilage repair by modulating immune responses via the inhibition of TLR4/NF-ĸB signaling.

Pharmacol Res 2020 05 11;155:104690. Epub 2020 Feb 11.

Department of Orthopedics, Renmin Hospital of Wuhan University, 430060 Wuhan, China.

The innate immune system drives inflammatory joint damage in osteoarthritis (OA) and regulates cartilage repair. Berberine chloride (BBR) is an isoquinoline alkaloid that shows immunomodulatory activity in a variety of cell lines. However, the immunomodulatory mechanisms of BBR in chondrocytes during OA are largely unknown. Herein, we assessed the ability of BBR to mediate chondroprotection through its effects on innate immunity. We found that BBR up-regulated the expression of surfactant protein D (SP-D) in OA cartilage, a key regulator of inflammation and innate immunity both in the airways and extrapulmonary tissues, including joint cartilage. To further explore these findings, we used recombinant adeno-associated virus (rAAV)-mediated knockdown of SP-D. Silencing was assessed in rat model of surgically-induced OA in the presence or absence of BBR treatment, 10 weeks post-surgery. We observed a clear improvement in histological scores of BBR-treated animals compared to those treated with BBR and the rAAV-SP-D vector. In addition, animals co-treated with BBR + recombinant human SP-D (rhSP-D) exhibited significantly lower histological scores than those treated with BBR alone. BBR treatment led to significantly reduced immune cell infiltration mediated through TLR4, F4/80, CD68 and CD34, whilst SP-D silencing reversed this improvement. In contrast, rhSP-D treatment enhanced the protective phenotype. We further explored how BBR influences SP-D and other OA-associated genes in vitro. We observed an up-regulation of SP-D and a marked decline in TRAF6, TLR4, MD-2 and MyD88 expression, as well as NF-κB p65 and IκBα phosphorylation in chondrocytes treated with sodium nitroprusside. siRNAs specific for SP-D were able to partially reverse this phenotype, whilst both rhSP-D and the TLR4 inhibitor TAK-242 enhanced the effects. Together, these results are consistent with a model wherein SP-D has therapeutic potential for OA treatment. Concomitantly, BBR modulates immune responses and decreases cartilage degradation. These findings suggest that BBR achieves this function through releasing SP-D from MD2/SP-D complexes and through the inhibition of TLR4/NF-κB signaling.
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http://dx.doi.org/10.1016/j.phrs.2020.104690DOI Listing
May 2020

Power ultrasound and its applications: A state-of-the-art review.

Ultrason Sonochem 2020 Apr 31;62:104722. Epub 2019 Oct 31.

Institute of Mathematics and Physics, Zhejiang Normal University, Zhejiang 321004, China. Electronic address:

Ultrasonic processing has attracted increasing attention by people because ultrasonic technology may represent a flexible 'green' alternative for energy efficient processes. The major challenges for the power ultrasound application in real situations are the design and development of specific power ultrasonic systems for large-scale operations. Thus, new families of power ultrasonic transducers have been developed in recent years to meet actual needs, and this contributes to the implementation of power ultrasound of application in many fields such as chemical industry, food industry and manufacturing. This paper presents the current state of ultrasonic transducers of magnetostrictiv type and piezoelectric type as well as applications of power ultrasound in various industrial fields including chemical reactions, drying/dehydration, welding, extraction, heat transfer enhancement, de-ice, enhanced oil recovery, droplet atomization, cleaning and fine particle removal. The review paper helps to understand the current development of power ultrasonic technology and its applications in various situations, and induce extended applications of power ultrasound to more and more fields.
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http://dx.doi.org/10.1016/j.ultsonch.2019.104722DOI Listing
April 2020

The development of the cricoid cartilage and its implications for the use of endotracheal tubes in the pediatric population.

Paediatr Anaesth 2020 01 9;30(1):63-68. Epub 2019 Dec 9.

Department of Anesthesia, Shengjing Hospital, China Medical University, Shenyang, China.

Background: The rigid cricoid cartilage is functionally the narrowest portion of the larynx. There is some controversy over the shape of the pediatric cricoid cartilage in the transverse plane. It is important to understand the development of the cricoid cartilage so that endo-traceheal tubes can be used more safely.

Aim: To determine changes in the internal diameter and shape of the cricoid cartilage during development and explore the implications of those changes for the selection of ETT type and size for children.

Methods: The cervical computed tomography scans were reviewed in patients aged 1-20 years. After performing the multiplanar reconstruction and correcting the slant, the transverse and anteroposterior internal diameters of the inlet and outlet of the cricoid cartilage were measured, respectively. The angle between the arch and the lamina of the cricoid cartilage in the middle sagittal plane was measured. The ratios of transverse to anteroposterior diameter for the inlet and outlet of the cricoid cartilage were calculated, respectively.

Results: In females, the internal diameters of the cricoid cartilage increased linearly with age. In males, the internal diameters of the cricoid cartilage exhibited a growth spurt during adolescence. The transverse diameter of the inlet was the smallest diameter of the cricoid cartilage, and the predicting formula of the transverse diameter of the inlet for children aged 1-12 was 0.4 × age (year) + 5.1, R  = .758. The angle between the arch and lamina of the cricoid cartilage and the ratios of transverse to anteroposterior diameter correlated weakly with age.

Conclusion: The transverse inner diameter of the inlet is the smallest diameter of the cricoid cartilage. The "funnel shape" of the cricoid cartilage remains unchanged during development. The outer diameter should be considered when selecting an endotracheal tube.
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http://dx.doi.org/10.1111/pan.13772DOI Listing
January 2020

Enhanced fluoride uptake by bimetallic hydroxides anchored in cotton cellulose/graphene oxide composites.

J Hazard Mater 2019 08 16;376:91-101. Epub 2019 May 16.

Office of Pollution Emission Control, Binzhou City, PR China.

A novel hybrid nanomaterial was synthesized by embedding the bimetallic Zr and La (hydro)xides onto the cotton cellulose/graphene oxide composites (CC/GO composites), forming the Zr-La-CC/GO nanocomposites. Selective uptake of fluoride onto the Zr-La /GO hybrids in multiple competitive environments were evaluated. Morphological characteristics of Zr-La-CC/GO nanocomposites reflected the well distributions of embedded Zr and La hydroxides in the nanocomposites. Results also indicated that the encapsulated bimetallic hydroxides in Zr-La-CC/GO hybrids exhibited extremely high fluoride adsorption capacity and stability. XPS investigation exhibited the strong ZrF and LaF bonds in spent Zr-La-CC/GO nanocomposites, and the bonds were weakened at higher pH, which was consistent with the adsorption results. In addition, CC/GO composites using as the host could also exert the strong shielding effect to improve the stability of embedded La and Zr species so as only a low La dissolution (<4.2%) and almost no Zr leaching (0.1%) were observed in high HA concentration. What's more, the Zr-La-CC/GO nanocomposites have also shown great potential application for defluoridation in field.
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http://dx.doi.org/10.1016/j.jhazmat.2019.05.039DOI Listing
August 2019

Optimization and kinetic study of methyl laurate synthesis using ionic liquid [Hnmp]HSO as a catalyst.

R Soc Open Sci 2018 Sep 12;5(9):180672. Epub 2018 Sep 12.

Faculty of Energy and Environmental Science, Yunnan Normal University, No. 768, Juxian Street, Chenggong District, Kunming 650500, Yunnan, People's Republic of China.

Methyl laurate was synthesized from lauric acid (LA) and methanol via an esterification reaction using ionic liquids (ILs) as catalysts. The efficiencies of three different catalysts, 1-methylimidazole hydrogen sulfate ([Hmim]HSO), 1-methyl-2-pyrrolidonium hydrogen sulfate ([Hnmp]HSO) and HSO, were compared. The effect of the methanol/LA molar ratio, reaction temperature, reaction time and catalyst dosage on the esterification rate of LA was investigated by single-factor experiments. Based on the single-factor experiments, the esterification of LA and methanol was optimized using response surface methodology. The results showed that the most effective catalyst was the IL [Hnmp]HSO. The optimal conditions were as follows: [Hnmp]HSO dosage of 5.23%, methanol/LA molar ratio of 7.68 : 1, reaction time of 2.27 h and reaction temperature of 70°C. Under these conditions, the LA conversion of the esterification reached 98.58%. A kinetic study indicated that the esterification was a second-order reaction with an activation energy and a frequency factor of 68.45 kJ mol and 1.9189 × 10 min, respectively. The catalytic activity of [Hnmp]HSO remained high after five cycles.
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http://dx.doi.org/10.1098/rsos.180672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170529PMC
September 2018

Ligustilide attenuates nitric oxide-induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways.

J Cell Mol Med 2019 05 15;23(5):3357-3368. Epub 2019 Feb 15.

Department of Orthopedics, Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.

Ligustilide (LIG) is the main lipophilic component of the Umbelliferae family of pharmaceutical plants, including Radix angelicae sinensis and Ligusticum chuanxiong. LIG shows various pharmacological properties associated with anti-inflammation and anti-apoptosis in several kinds of cell lines. However, the therapeutic effects of LIG on chondrocyte apoptosis remain unknown. In this study, we investigated whether LIG had an anti-apoptotic activity in sodium nitroprusside (SNP)-stimulated chondrocyte apoptosis and could delay cartilage degeneration in a surgically induced rat OA model, and elucidated the potential mechanisms. In vitro studies revealed that LIG significantly suppressed chondrocyte apoptosis and cytoskeletal remodelling, which maintained the nuclear morphology and increased the mitochondrial membrane potential. In terms of SNP, LIG treatment considerably reduced the expression levels of cleaved caspase-3, Bax and inducible nitric oxide synthase and increased the expression level of Bcl-2 in a dose-dependent manner. The LIG-treated groups presented a significantly suppressed expression of activating transcription factor 2 and phosphorylation of Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). The inhibitory effect of LIG was enhanced by the p38 MAPK inhibitor SB203580 or the JNK inhibitor SP600125 and offset by the agonist anisomycin. In vivo studies demonstrated that LIG attenuated osteoarthritic cartilage destruction by inhibiting the cartilage chondrocyte apoptosis and suppressing the phosphorylation levels of activating transcription factor 2, JNK and p38 MAPK. This result was confirmed by histological analyses, micro-CT, TUNEL assay and immunohistochemical analyses. Collectively, our studies indicated that LIG protected chondrocytes against SNP-induced apoptosis and delayed articular cartilage degeneration by suppressing JNK and p38 MAPK pathways.
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http://dx.doi.org/10.1111/jcmm.14226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484328PMC
May 2019

Predicting the Size of a Left Double-Lumen Tube for Asian Women Based on the Combination of the Diameters of the Cricoid Ring and Left Main Bronchus: A Randomized, Prospective, Controlled Trial.

Anesth Analg 2020 03;130(3):762-768

From the Departments of Anesthesiology.

Background: There are limited data about how to choose the correct size of a double-lumen tube (DLT). It is especially difficult to select an appropriate DLT for Asian women because of their small stature. The primary aim of this study was to compare the overall accuracy of the DLT selected based on the combination of transverse diameter of cricoid ring and the equivalent diameter of the left main bronchus (ED-LMB) with that based on the ED-LMB alone for Asian women.

Methods: In this study, 80 consecutive and blinded female patients were assigned randomly to 2 groups. The overall accuracy of the selection of DLT was compared between the Combined group and LMB group. The accuracy of the selection of tracheal segment and bronchial segment was also compared between the 2 groups. The postoperative hoarseness and sore throat were investigated by blinded assessors.

Results: The overall accuracy of selection of the DLTs was higher in the Combined group than that in the LMB group (87.5% vs 60.0%; P = .010). The accuracy of selection of tracheal segment was also higher in the Combined group (92.5% vs 67.5%; P = .010). The accuracy of selection of bronchial segment was similar between the groups (95.0 % vs 86.1%; P = .246). The severity of sore throat was higher in the LMB group at 24 hours after the operation (P = .001).

Conclusions: The correct size of DLT for Asian women should be selected based on the combination of transverse diameter of cricoid ring and the ED-LMB.
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http://dx.doi.org/10.1213/ANE.0000000000003839DOI Listing
March 2020

The Combination of Diameters of Cricoid Ring and Left Main Bronchus for Selecting the "Best Fit" Double-Lumen Tube.

J Cardiothorac Vasc Anesth 2018 04 22;32(2):869-876. Epub 2017 Nov 22.

Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China. Electronic address:

Objectives: The aims of this study were to measure diameters of the cricoid ring and left main bronchus in Asian adult patients and to assess the accuracy of double lumen tube size selected according to cricoid and left main bronchus diameter, respectively.

Design: Retrospective observational study.

Setting: Academic, tertiary care hospital.

Participants: Preoperative CT scans from 87 men and 94 women who had undergone general anesthesia for lung operations.

Interventions: No intervention.

Measurements And Main Results: The diameters of the cricoid ring and left main bronchus were measured from thoracic computed tomography images after correction of slant. The "best-fit" size of double lumen tube was determined by comparing diameter of the left main bronchus and cricoid ring with the diameter of the double lumen tube. Diameters of the cricoid ring and left main bronchus were both significantly greater in men compared with women (p < 0.0001). Shapes of cricoid rings were different between genders (p < 0.0001), while shapes of the left main bronchus were not significant different (p = 0.343). With reference to the "best fit" size, the rate of agreement of cricoid ring size, left main bronchus size, and height size for men were 100%, 100%, and 94.3%. For women, the rate of agreement of cricoid ring size, left main bronchus size, and height size were 94.7%, 63.8%, and 51.1%.

Conclusions: The "best fit" size of a double lumen tube should be decided by a combination of diameters of the cricoid ring and the left main bronchus.
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http://dx.doi.org/10.1053/j.jvca.2017.11.029DOI Listing
April 2018

Surfactant protein D attenuates nitric oxide-stimulated apoptosis in rat chondrocyte by suppressing p38 MAPK signaling.

Biochem Biophys Res Commun 2018 01 31;495(1):526-532. Epub 2017 Oct 31.

Department of Orthopedics, Central Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, China. Electronic address:

Innate immune molecule surfactant protein D (SP-D), a member of the C-type lectin protein family, plays an indispensable role in host defense and the regulation of inflammation in the lung and other tissues. Osteoarthritis (OA) is a degenerative disease of cartilage, with inflammation that causes pathologic changes and tissue damage. However, it is unknown whether there exist SP-D expression and its potential role in the pathogenesis of OA. In this study, we examined SP-D expression and explored its biological function in a sodium nitroprusside (SNP)-stimulated rat chondrocytes and surgically-induced rat OA model. We found SP-D expression in both human and rat articular chondrocytes, with higher level in normal chondrocytes compared to in OA chondrocytes. Furthermore, In vivo study demonstrated that recombinant human SP-D (rhSP-D) ameliorated cartilage degeneration in surgically-induced rat OA model. In vitro cell culture study showed that rhSP-D markedly inhibited the expression of caspase-3 as an apoptosis biomarker, and decreased phosphorylation of p38 mitogen-activated protein kinase (MAPK), which resulted in maintaining normal nuclear morphology and increasing mitochondrial membrane potential in SNP-stimulated rat chondrocytes. Collectively, these findings indicate that SP-D expresses in articular chondrocytes and suppresses SNP-stimulated chondrocyte apoptosis and ameliorates cartilage degeneration via suppressing p38 MAPK activity.
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http://dx.doi.org/10.1016/j.bbrc.2017.10.170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421565PMC
January 2018

In vitro study of accuracy of subaxial cervical pedicle screw insertion using calipers based on the gravity line.

PLoS One 2017 20;12(7):e0181324. Epub 2017 Jul 20.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, PR China.

Object: There is a high probability of iatrogenic perforation of the vertebral pedicle wall during the application of subaxial cervical pedicle screw (CPS). The goal of this study was to evaluate the accuracy of CPS insertion at C3-C7 in vitro using novel calipers based on the gravity line.

Methods: Nine cadaveric cervical spines underwent computed tomography scanning and preoperative design. A lateral fluoroscopic view was taken to measure the intra-operative sagittal angle by C-arm with hanging cross structured K-wires. By referring to the gravity line, caliper A was used to locate the entry point, while caliper B was employed to guide the screw insertion. Postoperative CT scans were performed to assess the accuracy of the screw placements, according to the Neo classification.

Results: Overall, 78 (88.6%) of the 88 pedicle screw placements were classified as grade 0 (correct position), 4 (4.5%) were grade 1 (non-critical perforation), 4 (4.5%) were grade 2 (critical perforation), and 2 (2.3%) were grade 3 (critical perforation).

Conclusions: Using our novel calipers and referring to the gravity line was helpful for locating and guiding individual cervical pedicle screw insertions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181324PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519080PMC
October 2017

Chitosan oligosaccharides protect nucleus pulposus cells from hydrogen peroxide-induced apoptosis in a rat experimental model.

Biomed Pharmacother 2017 Sep 14;93:807-815. Epub 2017 Jul 14.

Renmin Hospital of Wuhan University, Wuhan University, Zhang Zhidong Road, No. 9, Wuhan 430060, China. Electronic address:

Chitosan has been investigated for its protective effect on nucleus pulposus (NP) cells against intervertebral disc degeneration(IDD), but its high molecular weight prohibits its clinical application. The low molecular derivatives, chitosan oligosaccharides (COS) are easier to absorb; however, the protective effect of COS against IDD remains unclear. In this study, we investigated the effects of COS on NP degeneration. NP cells derived from rats were treated with HO to induce an IDD-like transition. Then, COS was used to pre-treat cells before administering HO and changes occurring in the cells were observed. As shown by the result of a cell counting kit-8(CCK-8) assay, COS protected the viability of the cells. The induced apoptosis rate fell when cells were pre-treated with COS, revealed by annexin-V FITC/PI double staining analysis and Hoechst 33342 staining. COS administration also protected ECM synthesis and prevented its degradation, as shown by western blotting(WB) and polymerase chain reaction(PCR). We analyzed the activity of the PI3K/Akt pathway in HO treated NP cells by WB and the result showed that COS could enhance activity of the pathway. To investigate the relationship between the PI3K/Akt pathway and the protective effects of COS on NP cells, the PI3K/Akt pathway was inhibited by wortmannin, and we subsequently found that this abolished the protective effects. These results support the hypothesis that COS exerts its protective effect on NP cells against HO-induced apoptosis via the PI3K/Akt pathway.
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http://dx.doi.org/10.1016/j.biopha.2017.06.101DOI Listing
September 2017

Is it cardiac involvement mimicking acute myocardial infarction in idiopathic inflammatory myopathy?

Exp Ther Med 2017 Jul 11;14(1):349-354. Epub 2017 May 11.

Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.

Idiopathic inflammatory myopathy (IIM) comprises a group of rare systemic diseases characterized by progressive weakness of the symmetrical proximal limb muscles, elevated muscle enzymes, inflammation or necrosis on muscle biopsy. IIM may impair the function of multiple organs, particularly the heart. However, it rarely manifests as acute myocardial infarction (AMI) at initial presentation. The present study described the case of a 39-year-old woman with AMI, whose muscle biopsy on the left arm conformed to polymyositis. Coronary arteriography showed irregularities in the left descending and right coronary artery (25% diameter reduction in middle segment). It was concluded that AMI was the chief manifestation of IIM at initial presentation. Furthermore, to the best of our knowledge, the present study was the first to provide a systematic literature review to assess AMI in IIM patients and discuss the possible causes of AMI.
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http://dx.doi.org/10.3892/etm.2017.4447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488513PMC
July 2017
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