Publications by authors named "Shijie Wang"

221 Publications

Characterization of tetracycline-resistant microbiome in soil-plant systems by combination of HO-based DNA-Stable isotope probing and metagenomics.

J Hazard Mater 2021 Jul 14;420:126440. Epub 2021 Jul 14.

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The emergence and spread of antibiotic resistance have been considered as a global health threat. However, effective methods to identify antibiotic-resistant bacteria (ARB) in complex microbial community are lacking, and the potential transmission pathways of ARB and antibiotic resistance genes (ARGs) in the soil-plant system remain scarce. Here in this study, tetracycline was chosen as the target antibiotic due to its globally wide usage and clinical significance. DNA-based stable isotope probing with HO was applied to identify the tetracycline-resistant bacteria from soil-plant systems. Eighteen-year organic fertilization significantly changed the composition of the tetracycline-resistant microbiome in the soil-wheat system and resulted in a higher relative abundance of ARGs in the wheat endophyte. Rhizosphere harboring the most diverse ARGs and mobile genetic elements was identified as a hot spot for horizontal gene transfer and an important bridge between bulk soil and wheat endophyte. Micrococcaceae and Sphingomonadaceae carrying ARGs associated with abundant mobile genetic elements, were identified as the core bacterial taxa in long-term manure-amended and untreated soil-wheat systems, respectively. This method contributes to a more precise track of ARB in the environment, and our work depicts the high potential of ARG transfer in the rhizosphere mediated by the core species.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126440DOI Listing
July 2021

Distinct Functions and Assembly Mechanisms of Soil Abundant and Rare Bacterial Taxa Under Increasing Pyrene Stresses.

Front Microbiol 2021 2;12:689762. Epub 2021 Jul 2.

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.

Elucidating the relative importance of species interactions and assembly mechanisms in regulating bacterial community structure and functions, especially the abundant and rare subcommunities, is crucial for understanding the influence of environmental disturbance in shaping ecological functions. However, little is known about how polycyclic aromatic hydrocarbon (PAH) stress alters the stability and functions of the abundant and rare taxa. Here, we performed soil microcosms with gradient pyrene stresses as a model ecosystem to explore the roles of community assembly in determining structures and functions of the abundant and rare subcommunities. The dose-effect of pyrene significantly altered compositions of abundant and rare subcommunities. With increasing pyrene stresses, diversity increased in abundant subcommunities, while it decreased in the rare. Importantly, the abundant taxa exhibited a much broader niche width and environmental adaptivity than the rare, contributing more to pyrene biodegradation, whereas rare taxa played a key role in improving subcommunity resistance to stress, potentially promoting community persistence and stability. Furthermore, subcommunity co-occurrence network analysis revealed that abundant taxa inclined to occupy the core and central position in adaptation to the pyrene stresses. Stochastic processes played key roles in the abundant subcommunity rather than the rare subcommunity. Overall, these findings extend our understanding of the ecological mechanisms and interactions of abundant and rare taxa in response to pollution stress, laying a leading theoretical basis that abundant taxa are core targets for biostimulation in soil remediation.
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http://dx.doi.org/10.3389/fmicb.2021.689762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283415PMC
July 2021

The Distribution and Origins of "Wild Plant With Tiny Population" Using Whole Genome Resequencing.

Front Plant Sci 2021 17;12:668796. Epub 2021 Jun 17.

Forest Department, Forestry College, Hebei Agricultural University, Baoding, China.

is a valuable but endangered wild resource in the genus . It has been listed as one of the 120 wild species with tiny population in China. The specie has been little studied. A preliminary study of propagation modes in was performed through seed propagation, hybridization, self-crossing trials, bud grafting, branch grafting, and investigations of natural growth. The results showed that the population size of was very small, the distribution range was limited, and the habitat was extremely degraded. In the wild population, natural hybridization and root tiller production were the major modes of propagation. Whole genome re-sequencing of the 23 wild and cultivated accessions from species collected was performed using an Illumina HiSeq sequencing platform. The sequencing depth range was 26.56x-44.85x and the average sequencing depth was 32x. Phylogenetic tree and principal component analyses (PCA) based on SNPs showed that the wild species, such as PWH06, PWH07, PWH09, PWH10, PWH13, and PWH17, were closely related to both HB-1 and HB-2. Using these results in combination with morphological characteristics, it speculated that populations may form a natural hybrid group with frequent gene exchanges between and within groups. A selective elimination analysis on the population were performed using Fst and π radio and a total of 381 overlapping genes including , and genes were obtained. These genes were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) function enrichment. And four KEGG pathways, including lysine degradation, sphingolipid metabolism, other glycan degradation, and betaine biosynthesis were significantly enriched in the population. Our study provided information on genetic variation, evolutionary relationships, and gene enrichment in population. These data will help reveal the evolutionary history and origin of and provide guidelines for subsequent research on the locations of functional genes.
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http://dx.doi.org/10.3389/fpls.2021.668796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250157PMC
June 2021

Combined transcriptome and proteome analysis provides insights into anthocyanin accumulation in the leaves of red-leaved poplars.

Plant Mol Biol 2021 Jun 24. Epub 2021 Jun 24.

Forest Department, Forestry College, Hebei Agricultural University, Baoding, China.

Key Message: Anthocyanin was highly accumulated in the leaves of red-leaved poplars; Many structural genes involved in anthocyanin synthesis were significantly up-regulated in 'Quanhong' and 'Xuanhong'; TTG2, HYH, and HY5 may be directly involved in the regulation of anthocyanin synthesis in both red-leaved poplars. The red-leaved poplar cultivars 'Quanhong' and 'Xuanhong' are bud mutations of Populus deltoides cv. 'Zhonglin 2025'. These cultivars are valued for their beautiful shape, lack of flying catkins, and ornamental leaf colors. However, the understanding of the molecular mechanism of anthocyanin accumulation in the leaves of red-leaved poplars is still unclear. Here, we profiled the changes of pigment content, transcriptome and proteome expression in the leaves of three poplar cultivars and the results showed that the ratios of anthocyanin to total chlorophyll in both red-leaved poplars were higher than that in 'Zhonglin 2025', indicating that the anthocyanin was highly accumulated in the leaves of red-leaved poplars. Based on the results of combined transcriptome and proteome analysis, 15 and 11 differentially expressed genes/proteins involved in anthocyanin synthesis were screened in 'Quanhong' and 'Xuanhong', respectively, indicating that the two red-leaved poplar cultivars have slightly different patterns of regulating anthocyanin biosynthesis. Among the 120 transcription factors, 3 (HY5, HYH, and TTG2), may be directly involved in the regulation of anthocyanin synthesis in both red-leaved poplars. This study screens the candidate genes involved in anthocyanin accumulation in the leaves of red-leaved poplars and lays a foundation for further exploring the molecular mechanism of leaf red coloration in red-leaved poplars.
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http://dx.doi.org/10.1007/s11103-021-01166-4DOI Listing
June 2021

Clinicopathological characteristics and prognosis of signet ring cell carcinoma of the gallbladder.

BMC Gastroenterol 2021 Jun 6;21(1):248. Epub 2021 Jun 6.

Department of Hepatobiliary and Pancreatovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China.

Background: Signet ring cell carcinoma (SRC) is a rare histological subtype of gallbladder adenocarcinoma. The current study evaluates the clinicopathologic features and prognosis of SRC.

Methods: Patients with adenocarcinoma of the gallbladder were identified in the Surveillance, Epidemiology, and End Results database from 1973 to 2016. Overall survival (OS) and cancer-specific survival (CSS) of patients who had SRC were compared with those of patients who had non-SRC using Cox regression and propensity score methods.

Results: Of 22,781 gallbladder adenocarcinomas retrieved, 377 (1.7%) were SRC and the other 22,404 were non-SRC. SRC was more significantly associated with older age, female gender, poor differentiation, advanced tumor stage, lymph node metastasis, distant metastasis, and advanced AJCC stage. The 5-year OS and CSS in the SRC group were 7.2 and 6.5%, respectively, both of which were significantly worse than the 13.2 and 13.3% seen in the SRC group (P = 0.002 and P = 0.012, respectively). This survival disadvantage persisted in multivariable analyses [hazard ratio (HR) = 1.256, P = 0.021 and HR = 1.211, P = 0.036] and after propensity score matching (OS: HR = 1.341, P = 0.012 and CSS: HR = 1.625, P = 0.005). Surgery in combination with chemotherapy improved OS of gallbladder SRC patients compared with surgery alone (HR = 0.726, P = 0.036) or chemotherapy alone (HR = 0.433, P < 0.001).

Conclusion: Patients with SRC of the gallbladder have distinct clinicopathological features with poor prognosis. Surgery in combination with chemotherapy can improve survival.
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http://dx.doi.org/10.1186/s12876-021-01831-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180115PMC
June 2021

The responses of weathering carbon sink to eco-hydrological processes in global rocks.

Sci Total Environ 2021 Sep 14;788:147706. Epub 2021 May 14.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, Guizhou Province, China; School of Geography and Environmental Sciences, Guizhou Normal University, Guiyang 550001, China.

Eco-hydrological processes affect the chemical weathering carbon sink (CS) of rocks. However, due to data quality limitations, the magnitude of the CS of rocks and their responses to eco-hydrological processes are not accurately understood. Therefore, based on Global Erosion Model for CO fluxes (GEM-CO model), hydrological site data, and multi-source remote sensing data, we produced a 0.05° × 0.05° resolution dataset of CS for 11 types of rocks from 2001 to 2018. The results show that the total amount of CS of global rocks is 0.32 ± 0.02 Pg C, with an average flux of 2.7 t C km yr, accounting for 53% and 3% of the "missing" carbon sink and fossil fuel emissions, respectively. This is 23% higher than previous research results, which may be due to the increased resolution. Although about 60% of the CS of global rocks are in a stable state, there are obvious differences among rocks. For example, the CS of carbonate rocks exhibited a significant increase (0.30 Tg C/yr), while the CS of siliceous clastic sedimentary rocks exhibited a significant decrease (-0.06 Tg C/yr). Although temperature is an important factor affecting the CS, the proportion of soil moisture in arid and temperate climate zones is higher (accounting for 24%), which is 3.6 times that of temperature. Simulations based on representative concentration pathways scenarios indicate that the global CS of rocks may increase by about 28% from 2050 to 2100. In short, we produced a set of high-resolution datasets for the CS of global rocks, which makes up for the lack of datasets in previous studies and improves our understanding of the magnitude and spatial pattern of the CS and its responses to eco-hydrological processes.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147706DOI Listing
September 2021

Studies on chemoselective synthesis of 1,4- and 1,2-dihydropyridine derivatives by a Hantzsch-like reaction: a combined experimental and DFT study.

Org Biomol Chem 2021 May;19(17):3882-3892

Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, P. R. China.

In the experimental process of preparing diethyl 3,5-dicarboxylate-1,4-dihydropyridine (1,4-DHP) by a Hantzsch-like reaction, it was found that a by-product named diethyl 3,5-dicarboxylate-1,2-dihydropyridine (1,2-DHP) was produced in the reaction. To discuss this phenomenon, the effects of the reaction conditions on the yield ratio of 1,4-DHP and 1,2-DHP were studied by using aromatic amines, aromatic aldehydes and ethyl propiolate as raw materials. The mechanisms for the formation of 1,4-DHP and 1,2-DHP were proposed based on the isolated intermediate named diethyl 4-((phenylamino)methylene)pent-2-enedioate generated by the Michael addition of aniline and ethyl propiolate. The transition state structures were optimized and the reaction energy barriers of intermediates in the speculated mechanisms were calculated by DFT calculations at the M062X/def2TZVP//B3LYP-D3/def-SVP level. It was found that the reaction energy barriers and dominant configurations of intermediates IM2 and IM3' are the determinants for the chemoselectivity. Together, these results demonstrate a high chemoselectivity in the synthesis of 1,4-DHPs and 1,2-DHPs by a Hantzsch-like reaction and that 1,4-DHPs and 1,2-DHPs can be easily obtained under different conditions.
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http://dx.doi.org/10.1039/d0ob02289fDOI Listing
May 2021

Tethered and Untethered 3D Microactuators Fabricated by Two-Photon Polymerization: A Review.

Micromachines (Basel) 2021 Apr 20;12(4). Epub 2021 Apr 20.

Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Sha Tin, Hong Kong 999077, China.

Microactuators, which can transform external stimuli into mechanical motion at microscale, have attracted extensive attention because they can be used to construct microelectromechanical systems (MEMS) and/or microrobots, resulting in extensive applications in a large number of fields such as noninvasive surgery, targeted delivery, and biomedical machines. In contrast to classical 2D MEMS devices, 3D microactuators provide a new platform for the research of stimuli-responsive functional devices. However, traditional planar processing techniques based on photolithography are inadequate in the construction of 3D microstructures. To solve this issue, researchers have proposed many strategies, among which 3D laser printing is becoming a prospective technique to create smart devices at the microscale because of its versatility, adjustability, and flexibility. Here, we review the recent progress in stimulus-responsive 3D microactuators fabricated with 3D laser printing depending on different stimuli. Then, an outlook of the design, fabrication, control, and applications of 3D laser-printed microactuators is propounded with the goal of providing a reference for related research.
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http://dx.doi.org/10.3390/mi12040465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074609PMC
April 2021

ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM.

Theranostics 2021 3;11(12):5926-5938. Epub 2021 Apr 3.

The State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.

Metabolic reprogramming, especially Warburg effect, is a key event in tumor initiation and progression. ZEB1 plays a vital role in metastasis of various cancers. We previously found that ZEB1 was excessively expressed in hepatocellular carcinoma (HCC) and its high expression was closely correlated with metastasis and recurrence of HCC. We want to know whether glycolytic enzymes are regulated by ZEB1 and contribute to carcinogenesis and metastasis of HCC. To explore whether ZEB1 could enhance glycolysis in HCC, we knocked down ZEB1 by short hairpin RNA (shRNA) in MHCC-97H and HCC-LM3 cells and performed glucose uptake, lactate production, ECAR and OCR assays. To investigate how ZEB1 enhances glycolysis, the protein levels of glycolytic enzymes were detected in the same cell lines using Western blot. The regulatory effect of ZEB1 on PFKM mRNA level was confirmed by RT-qPCR, luciferase report assay and ChIP assay. In order to assess the role of ZEB1-PFKM axis in cell proliferation, cell counting and CCK-8 assays were performed in MHCC-97H and HCC-LM3 cell lines knocked down for ZEB1 and further re-expressed for either ZEB1 or PFKM or not. To explored whether the ZEB1-PFKM axis also functions in HCC cell migration, invasion and metastasis, the same MHCC-97H and HCC-LM3 cell lines were performed for wound healing assays, transwell assays and colony formation assays, meanwhile, MHCC-97H cell lines were performed for orthotopic liver transplantation assays. Finally, the expression of ZEB1 and PFKM were examined in human liver cancer specimens and non-tumorous liver tissues using immunohistochemical and Western blot. We found that ZEB1 transcriptionally upregulates the expression of the muscle isoform of phosphofructokinase-1 (PFKM), a rate-limiting enzyme in glycolysis. Intriguingly, a non-classic ZEB1-binding sequence in the promoter region of PFKM was identified through which ZEB1 directly activates the transcription of PFKM. Silencing of ZEB1 in MHCC-97H and HCC-LM3 cell leads to impaired PFKM expression, glycolysis, proliferation and invasion, and such impairments are rescued by exogenous expression of PFKM. Importantly, HCC xenograft assays and studies from TCGA database demonstrate that ZEB1-PFKM axis is crucial for carcinogenesis and metastasis of HCC. Our study reveals a novel mechanism of ZEB1 in promoting HCC by activating the transcription of PFKM, establishing the direct link of ZEB1 to the promotion of glycolysis and Warburg effect and suggesting that inhibition of ZEB1 transcriptional activity toward PFKM may be a potential therapeutic strategy for HCC.
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http://dx.doi.org/10.7150/thno.56490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058737PMC
July 2021

Previously identified and unidentified polybrominated biphenyl congeners in serum from people living in an electronic waste dismantling area in China.

Chemosphere 2021 Sep 8;279:130478. Epub 2021 Apr 8.

College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China; Engineering Research Center of Food Environment and Public Health, Beijing, 100081, China. Electronic address:

The effects of polybrominated biphenyls (PBBs) on human health have previously attracted much attention, but recent studies of PBBs have been focused on BB-153 and a few other congeners. PBB concentrations in serum samples from residents of an area containing an electronic waste dismantling site were determined in this study. The total PBB concentrations (i.e., the sums of the concentrations of the 35 PBB congeners) were 229-1360 ng/g lipid. The BB-153 concentrations were markedly higher in the samples from people living in the electronic waste dismantling area than in samples from people living in a nearby control area. BB-153 was found in all of the samples from the study exposure area but the concentrations were relatively low (0.07-4.70 ng/g lipid). High BB-1 concentrations were found for the first time in serum from people living in both the electronic waste dismantling and control areas. The BB-1 concentrations were 211-1280 ng/g lipid. The retention times of the 35 PBB standards and PCBs (polychlorinated biphenyls) with similar structures were used to predict the retention times of unidentified PBB congeners to allow the PBB distributions in the serum samples to be identified. A total of 26 previously unidentified PBB congeners were identified in the human serum samples. BB-5, BB-35, BB-79, and BB-109 were found in >50% of the samples. The PBB patterns in the serum samples were different from the patterns previously found in serum after a PBB contamination incident in 1973, so the health risks currently posed by PBBs are worth studying.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130478DOI Listing
September 2021

Surface defects induced charge imbalance for boosting charge separation and solar-driven photocatalytic hydrogen evolution.

J Colloid Interface Sci 2021 Aug 24;596:12-21. Epub 2021 Mar 24.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People's Republic of China; School of Chemistry and Materials Science, Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin 150080, People's Republic of China. Electronic address:

Low charge separation efficiency of semiconductor materials is the main obstacle for high-performance photocatalyst. Herein, we report surface defects engineered uniform mesoporous TiO nanospheres (DMTNSs) through surfactant-mediated self-assembly solvothermal approach combined with hydrogenation strategy to promote charge separation. The surface defects induced charge imbalance result in the formation of built-in field, which can promote photogenerated charge separation efficiently and be confirmed by experimental and density functional theory (DFT) calculations. Under AM 1.5G irradiation, the photocatalytic hydrogen evolution of DMTNSs is ~3.34 mmol h g, almost 3.5 times higher than that of pristine non-defective TiO nanospheres (0.97 mmol h g), due to the engineered surface defects narrowing the bandgap (~3.01 eV) and inducing charge imbalance to boost spatial charge separation and extend visible-light response. The defect induced charge imbalance strategy opens a new valuable perspective for fabricating other high-efficient oxide photocatalysts.
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http://dx.doi.org/10.1016/j.jcis.2021.03.116DOI Listing
August 2021

All-fiber pulsed laser generation of 25.5  mJ and solution of parasitic oscillation.

Appl Opt 2021 Feb;60(5):1117-1120

A Q-switched, high-energy pulsed master oscillator power amplifier fiber laser utilizing the lab-built 100/400 µm double-cladding Yb-doped fiber is demonstrated. After two-stage amplification, the pulse energy was boosted to 25.5 mJ, for an average power of 510 W at a repetition of 20 kHz, yielding a slope efficiency of approximately 72.8%; the pulse duration was approximately 140 ns, and corresponding peak power was 182.1 kW. What is more, the limitation of further promotion of pulse energy was proposed: the threshold-like parasitic oscillation, which was determined by the injecting power, repetition, and fiber length, was the main restriction on power scaling in ultra-high-energy systems. Efficient solutions were proposed to suppress the parasitic oscillation by experimentally studies.
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http://dx.doi.org/10.1364/AO.409564DOI Listing
February 2021

Heterogeneity in α-synuclein fibril activity correlates to disease phenotypes in Lewy body dementia.

Acta Neuropathol 2021 04 28;141(4):547-564. Epub 2021 Feb 28.

Department of Pharmacology and Cancer Biology, Duke Center for Neurodegeneration Research, Duke University, 3 Genome Court, Durham, NC, 27710, USA.

α-Synuclein aggregation underlies pathological changes in Lewy body dementia. Recent studies highlight structural variabilities associated with α-synuclein aggregates in patient populations. Here, we develop a quantitative real-time quaking-induced conversion (qRT-QuIC) assay to measure permissive α-synuclein fibril-templating activity in tissues and cerebrospinal fluid (CSF). The assay is anchored through reference panels of stabilized ultra-short fibril particles. In humanized α-synuclein transgenic mice, qRT-QuIC identifies differential levels of fibril activity across the brain months before the deposition of phosphorylated α-synuclein in susceptible neurons. α-Synuclein fibril activity in cortical brain extracts from dementia with Lewy bodies (DLB) correlates with activity in matched ventricular CSF. Elevated α-synuclein fibril activity in CSF corresponds to reduced survival in DLB. α-Synuclein fibril particles amplified from cases with high fibril activity show superior templating in the formation of new inclusions in neurons relative to the same number of fibril particles amplified from DLB cases with low fibril activity. Our results highlight a previously unknown broad heterogeneity of fibril-templating activities in DLB that may contribute to disease phenotypes. We predict that quantitative assessments of fibril activities in CSF that correlate to fibril activities in brain tissue will help stratify patient populations as well as measure therapeutic responses to facilitate the development of α-synuclein-targeted therapeutics.
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http://dx.doi.org/10.1007/s00401-021-02288-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055043PMC
April 2021

Icotinib versus Cisplatin Plus Docetaxel as Adjuvant Chemotherapy in Patients with Stage II (N1+) Non-Small Cell Lung Cancer Harboring Positive EGFR Mutations: A Single-Center Retrospective Study.

Onco Targets Ther 2021 16;14:1083-1091. Epub 2021 Feb 16.

Department of Thoracic Surgery, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, People's Republic of China.

Purpose: The superior efficacy of first-line treatment with icotinib over that of standard chemotherapy has been well demonstrated in patients with advanced non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutation. However, whether icotinib is superior to cisplatin plus docetaxel as adjuvant chemotherapy in patients with stage II (N1+) NSCLC selected by EGFR mutation is controversial.

Methods: A total of 43 patients with completely resected stage II (T1-2N1M0) NSCLC and proven sensitive EGFR mutation (19Del or L858R) between January 2010 and December 2019 were included in our study. The disease-free survival (DFS) and overall survival (OS) were analyzed in 22 patients treated with icotinib and 21 patients treated with cisplatin plus docetaxel. Factors affecting DFS and OS were assessed by the Kaplan-Meier (KM) estimator and univariate Cox regression analysis.

Results: Our cohort included 22 icotinib patients and 21 cisplatin plus docetaxel patients with a median follow-up of 35.5 months and 38 months, respectively. Survival time was significantly longer in the icotinib group than in the chemotherapy group, with a median DFS of 47 months (95% CI, not reached) versus 18 months (95% CI, 12.4-23.6; HR 0.16; 95% CI, 0.07-0.35; log-rank p<0.0001). In the icotinib group, the most common adverse effects (AEs) were skin rash (40.9%) and elevated alanine aminotransferase (22.7%), whereas in the cisplatin plus docetaxel group, the most common AEs were nausea or vomiting (90.5%), anorexia (71.4%), and fatigue (71.4%). No deaths were treatment-related.

Conclusion: In this study, we demonstrated that in EGFR mutation-positive patients with completely resected stage II (T1-2N1M0) NSCLC, icotinib might provide DFS benefits, and reduced drug toxicity compared to cisplatin plus docetaxel. Thus, icotinib may be a reasonable option for adjuvant chemotherapy in patients with pathological stage II (N1+) NSCLC with EGFR mutation.
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http://dx.doi.org/10.2147/OTT.S290636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896780PMC
February 2021

Functional metagenomic and enrichment metatranscriptomic analysis of marine microbial activities within a marine oil spill area.

Environ Pollut 2021 Apr 27;274:116555. Epub 2021 Jan 27.

School of Chemical Engineering, Hebei University of Technology, Tianjin, 300130, China.

Microorganisms can degrade petroleum hydrocarbons, providing the advantages of low cost and few side effects towards ecosystems. Here, we evaluated the mechanisms of microbial degradation of marine petroleum hydrocarbon using metagenomics and metatranscriptomics approaches in order to provide new insight into microbial degradation of petroleum hydrocarbon. Seawater samples were collected at a depth of ∼8 m from an area near a drilling platform in the Bohai Bay and metagenomic sequencing was used to evaluate the functional potential of these marine microbial communities. Metatranscriptomic sequencing, fluorescence in-situ hybridization experiments, and flow cytometry were also performed on the microbial communities of samples subjected to 12 different culture conditions. The data were also subjected to Weighted Gene Co-expression Network Analysis (WGCNA) and co-transcription data visualization to evaluate co-transcription of gene functions. Metagenomic sequencing indicated the presence of numerous genes that were related to petroleum hydrocarbon metabolism. Further, the high co-transcription of genes in multiple pathways, indicated that groups of genes were synergistically transcribed to metabolize petroleum hydrocarbons. Metatranscriptomics also showed that microbial metabolism was highly active in the enrichments and that the transcription of a large number of prokaryotic replication and repair genes were significantly up-regulated including those encoding for the type VI secretion system (T6SS) protein, DNA polymerase I, thymidine phosphorylase, mevalonate kinase, and two-component systems. Concomitantly, the transcription of ribosomal genes involved in translation and photosynthetic genes involved in energy metabolism were down-regulated. Overall, oil and oxygen presence can increase the oil-degradation rates and related genes' transcription. Lot different metabolisms are co-regulated to exploit nutrients derived from the metabolism of petroleum hydrocarbons. Our analysis of metagenomic, metatranscriptomic and degradation data in this study show that a widespread gene spectrum involved in oil-degradation and the cooperation among genes is of great importance.
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http://dx.doi.org/10.1016/j.envpol.2021.116555DOI Listing
April 2021

Anaerobic degradation of xenobiotic organic contaminants (XOCs): The role of electron flow and potential enhancing strategies.

J Environ Sci (China) 2021 Mar 17;101:397-412. Epub 2020 Sep 17.

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

In groundwater, deep soil layer, sediment, the widespread of xenobiotic organic contaminants (XOCs) have been leading to the concern of human health and eco-environment safety, which calls for a better understanding on the fate and remediation of XOCs in anoxic matrices. In the absence of oxygen, bacteria utilize various oxidized substances, e.g. nitrate, sulphate, metallic (hydr)oxides, humic substance, as terminal electron acceptors (TEAs) to fuel anaerobic XOCs degradation. Although there have been increasing anaerobic biodegradation studies focusing on species identification, degrading pathways, community dynamics, systematic reviews on the underlying mechanism of anaerobic contaminants removal from the perspective of electron flow are limited. In this review, we provide the insight on anaerobic biodegradation from electrons aspect - electron production, transport, and consumption. The mechanism of the coupling between TEAs reduction and pollutants degradation is deconstructed in the level of community, pure culture, and cellular biochemistry. Hereby, relevant strategies to promote anaerobic biodegradation are proposed for guiding to an efficient XOCs bioremediation.
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http://dx.doi.org/10.1016/j.jes.2020.08.030DOI Listing
March 2021

Exosome markers of LRRK2 kinase inhibition.

NPJ Parkinsons Dis 2020 Nov 13;6(1):32. Epub 2020 Nov 13.

Department of Pharmacology and Cancer Biology, Duke Center for Neurodegeneration Research, Duke University, Durham, NC, USA.

Hyper-activated LRRK2 is linked to Parkinson's disease susceptibility and progression. Quantitative measures of LRRK2 inhibition, especially in the brain, maybe critical in the development of successful LRRK2-targeting therapeutics. In this study, two different brain-penetrant and selective LRRK2 small-molecule kinase inhibitors (PFE-360 and MLi2) were orally administered to groups of cynomolgus macaques. Proposed pharmacodynamic markers in exosomes from urine and cerebrospinal fluid (CSF) were compared to established markers in peripheral blood mononuclear cells (PBMCs). LRRK2 kinase inhibition led to reductions in exosome-LRRK2 protein and the LRRK2-substrate pT73-Rab10 in urine, as well as reduced exosome-LRRK2 and autophosphorylated pS1292-LRRK2 protein in CSF. We propose orthogonal markers for LRRK2 inhibition in urine and CSF can be used in combination with blood markers to non-invasively monitor the potency of LRRK2-targeting therapeutics.
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http://dx.doi.org/10.1038/s41531-020-00138-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666125PMC
November 2020

The stage-specific roles of radiotherapy and chemotherapy in nodular lymphocyte predominant Hodgkin lymphoma patients: a propensity score-matched analysis of the SEER database.

Cancer Med 2021 01 28;10(2):540-551. Epub 2020 Nov 28.

Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.

Background: The stage-specific roles of radiotherapy (RT) alone, chemotherapy alone, and combined RT and chemotherapy (CRT) for patients with nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) have not been adequately evaluated.

Methods: We analyzed patients with all stages of NLPHL enrolled in the Surveillance, Epidemiology, and End Results (SEER) registry from January 2000 to December 2015. Propensity score (PS) analysis with 1:1 matching (PSM) was performed to ensure the well-balanced characteristics of the comparison groups. Kaplan-Meier and Cox proportional-hazards models were used to evaluate the overall survival (OS), cancer-specific survival (CSS), hazard ratios (HRs), and corresponding 95% confidence intervals (95% CI). Restricted mean survival times (RMST) were also used for the survival analyses.

Results: For early-stage patients, CRT was associated with the best survival, the mean OS was significantly improved by approximately 20 months (20 m), and the risk of death was reduced by more than 80%, both before and after PSM (p < 0.05). For advanced-stage patients, none of RT alone, chemotherapy alone, or CRT had a significant effect on survival. Chemotherapy alone and CRT might be more beneficial for long-term survival (RMST : neither RT nor chemotherapy vs. chemotherapy alone vs. CRT = 104 m vs. 111 m vs. 108 m). Subgroup analysis of patients with early-stage NLPHL showed that CRT was associated with better survival of elderly patients (improved OS = 43.8 m, HR = 0.14, p < 0.05). However, the survival benefits of treatments for young patients were not statistically significant. The efficacy of RT was significantly different between the age groups (p  = 0.020).

Conclusions: These results from SEER data suggest that CRT may be considered for early-stage NLPHL, especially for elderly patients. Further studies are needed to identify effective treatments in patients with advanced-stage NLPHL.
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http://dx.doi.org/10.1002/cam4.3620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877359PMC
January 2021

Graphene-Based Helical Micromotors Constructed by "Microscale Liquid Rope-Coil Effect" with Microfluidics.

ACS Nano 2020 Oct 29. Epub 2020 Oct 29.

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.

Nature provides diverse inspirations for constructing mobile and functionalized micromachines. For example, artificial helical micro-/nanomotors inspired by bacteria flagella that can be precisely steered for various applications have been constructed by utilizing materials with excellent functions. Graphene-based materials show outstanding properties, and, to date, have not been considered to construct helical micromotors and investigate their potential applications. Here, we propose an interesting "microscale liquid rope-coil effect" strategy to stably and simply fabricate graphene oxide-based helical micromotors (GOFHMs) with high throughput by the capillary microfluidics technique. A range of desirable GOFHMs with different pitch, length, and linear diameter are tailored by smart parameter setting in microfluidic system (flow velocity, concentration, and so on). Afterward, graphene-based helical micromotors (GFHMs) are easily acquired by the reduction of GOFHMs and further drying. Actuated by rotating magnetic field, GFHMs show capability to conduct programmed locomotion in a microchannel. As a proof-of-concept demonstration, GFHMs and Ag modified GFHMs have been successfully applied to water remediation, which exhibits excellent removal efficiency of chemical and biological pollutants. Meanwhile, doxorubicin is modified onto GFHMs for the application of drug delivery. Accordingly, we believe that GFHMs have great potential in a variety of fields by modifying graphene with other nanoparticles or functional molecules.
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http://dx.doi.org/10.1021/acsnano.0c07067DOI Listing
October 2020

Exciton-Enabled Meta-Optics in Two-Dimensional Transition Metal Dichalcogenides.

Nano Lett 2020 Nov 15;20(11):7964-7972. Epub 2020 Oct 15.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, #08-03, Innovis, Singapore 138634, Singapore.

Optical wavefront engineering has been rapidly developing in fundamentals from phase accumulation in the optical path to the electromagnetic resonances of confined nanomodes in optical metasurfaces. However, the amplitude modulation of light has limited approaches that usually originate from the ohmic loss and absorptive dissipation of materials. Here, an atomically thin photon-sieve platform made of MoS multilayers is demonstrated for high-quality optical nanodevices, assisted fundamentally by strong excitonic resonances at the band-nesting region of MoS. The atomic thin MoS significantly facilitates high transmission of the sieved photons and high-fidelity nanofabrication. A proof-of-concept two-dimensional (2D) nanosieve hologram exhibits 10-fold enhanced efficiency compared with its non-2D counterparts. Furthermore, a supercritical 2D lens with its focal spot breaking diffraction limit is developed to exhibit experimentally far-field label-free aberrationless imaging with a resolution of ∼0.44λ at λ = 450 nm in air. This transition-metal-dichalcogenide (TMDC) photonic platform opens new opportunities toward future 2D meta-optics and nanophotonics.
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http://dx.doi.org/10.1021/acs.nanolett.0c02712DOI Listing
November 2020

Multi-level flash memory device based on stacked anisotropic ReS-boron nitride-graphene heterostructures.

Nanoscale 2020 Sep 7;12(36):18800-18806. Epub 2020 Sep 7.

State Key Laboratory of Precision Measurement Technology and Instruments, School of Precision Instruments and Opto-electronics Engineering, Tianjin University, No. 92 Weijin Road, Tianjin, 300072, China.

Charge-trapping memory devices based on two-dimensional (2D) material heterostructures possess an atomically thin structure and excellent charge transport capability, making them promising candidates for next-generation flash memories to achieve miniaturized size, high storage capacity, fast switch speed, and low power consumption. Here, we report a nonvolatile floating-gate memory device based on an ReS/boron nitride/graphene heterostructure. The implemented ReS memory device displays a large memory window exceeding 100 V, leading to an ultrahigh current ratio over 10 between programming and erasing states. The ReS memory device also exhibits an ultrafast switch speed of 1 μs. In addition, the device can endure hundreds of switching cycles and shows stable retention characteristics with ∼40% charge remaining after 10 years. More importantly, taking advantage of its anisotropic electrical properties, a single ReS flake can achieve direction-sensitive multi-level data storage to enhance the data storage density. On the basis of these characteristics, the proposed ReS memory device is potentially able to serve the entire memory device hierarchy, meeting the need for scalability, capacity, speed, retention, and endurance at each level.
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http://dx.doi.org/10.1039/d0nr03965aDOI Listing
September 2020

Response of the weathering carbon sink in terrestrial rocks to climate variables and ecological restoration in China.

Sci Total Environ 2021 Jan 4;750:141525. Epub 2020 Aug 4.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, Guizhou Province, China; Puding Karst Ecosystem Observation and Research Station, Chinese Academy of Sciences, Puding 562100, Guizhou Province, China; School of Geography and Environmental Sciences, Guizhou Normal University, Guiyang 550001, China.

The weathering carbon sink (CS) of rocks has a sensitive response to different influencing factors, and it is important to accurately distinguish this response in the global carbon cycle. However, no quantitative analysis of the response mechanism has been performed. In this study, the CS of the 12 types of terrestrial rocks in China from 2000 to 2014 is estimated using the GEM-CO model. The relative contribution rates of climate change and ecological restoration to the CS are quantitatively evaluated using the Lindeman-Merenda-Gold model. Results showed that: (1) The CS of terrestrial rocks in China was 17.69 Tg C yr, and the CS flux (CSF) was 2.53 t C km yr; mixed sedimentary rocks had the highest CS (6.89 Tg C yr), and carbonate rocks had the highest CSF (5.8 t C km yr). (2) The average annual CSF slightly decreased at a rate of 5.4 kg C km yr; the areas of the CSF that decreased in the south were the areas where water budget decreased significantly, and it was the areas with a reduced water budget and ecological deterioration in the north. (3) The relative contribution rates of water budget and precipitation reached 57% and 35%, respectively; the response of the CSF to temperature was evident in areas with low or decreasing temperatures, and the influence of fractional vegetation cover (FVC) on the CSF in low value area was evident. (4) Mixed sedimentary rocks and carbonate rocks displayed a more evident reduction trend in the CSF than other rocks. This research verified the applicability of the GEM-CO model in China and presented a scientific basis for quantitative assessment of the impact of climate change and ecological restoration on the CSF.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141525DOI Listing
January 2021

Characterization of landfill leachate by spectral-based surrogate measurements during a combination of different biological processes and activated carbon adsorption.

Water Sci Technol 2020 Jun;81(12):2606-2616

LIWET, Department of Green Chemistry and Technology, Ghent University, Campus Kortrijk, Graaf Karel De Goedelaan 5, B-8500 Kortrijk, Belgium.

Surrogate measurements based on excitation-emission matrix fluorescence spectra (EEMs) and ultraviolet-visible absorption spectra (UV-vis) were used to monitor the evolution of dissolved organic matter (DOM) in landfill leachate during a combination of biological and physical-chemical treatment consisting of partial nitritation-anammox (PN-Anammox) or nitrification-denitrification (N-DN) combined with granular active carbon adsorption (GAC). PN-Anammox resulted in higher nitrogen removal (81%), whereas N-DN required addition of an external carbon source to increase nitrogen removal from 24% to 56%. Four DOM components (C1 to C4) were identified by excitation-emission matrix-parallel factor analysis (EEM-PARAFAC). N-DN showed a greater ability to remove humic-like components (C1 and C3), while the protein-like component (C4) was better removed by PN-Anammox. Both biological treatment processes showed limited removal of the medium molecular humic-like component (C2). In addition, the synergistic effect of biological treatments and adsorption was studied. The combination of PN-Anammox and GAC adsorption could remove C4 completely and also showed a good removal efficiency for C1 and C2. The Thomas model of adsorption revealed that GAC had the maximum adsorption capacity for PN-Anammox treated leachate. This study demonstrated better removal of nitrogen and fluorescence DOM by a combination of PN-Anammox and GAC adsorption, and provides practical and technical support for improved landfill leachate treatment.
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http://dx.doi.org/10.2166/wst.2020.317DOI Listing
June 2020

MoS /Polymer Heterostructures Enabling Stable Resistive Switching and Multistate Randomness.

Adv Mater 2020 Oct 26;32(42):e2002704. Epub 2020 Aug 26.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), Innovis, 2 Fusionopolis Way, Singapore, 138634, Singapore.

Resistive random-access memories (ReRAMs) based on transition metal dichalcogenide layers are promising physical sources for random number generation (RNG). However, most ReRAM devices undergo performance degradation from cycle to cycle, which makes preserving a normal probability distribution during operation a challenging task. Here, ReRAM devices with excellent stability are reported by using a MoS /polymer heterostructure as active layer. The stability enhancement manifests in outstanding cumulative probabilities for both high- and low-resistivity states of the memory cells. Moreover, the intrinsic values of the high-resistivity state are found to be an excellent source of randomness as suggested by a Chi-square test. It is demonstrated that one of these cells alone can generate ten distinct random states, in contrast to the four conventional binary cells that would be required for an equivalent number of states. This work unravels a scalable interface engineering process for the production of high-performance ReRAM devices, and sheds light on their promising application as reliable RNGs for enhanced cybersecurity in the big data era.
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http://dx.doi.org/10.1002/adma.202002704DOI Listing
October 2020

Radiotherapy for patients with stage IV classical Hodgkin lymphoma: a propensity-matched analysis of the surveillance, epidemiology, and end results database.

Cancer Biol Ther 2020 09 23;21(9):832-840. Epub 2020 Aug 23.

Cancer Center, Renmin Hospital of Wuhan University , Wuhan, China.

Background The survival advantage of radiotherapy for patients with stage IV classic Hodgkin lymphoma (HL) has not been adequately evaluated. Methods We analyzed patients with stage IV HL enrolled from the Surveillance, Epidemiology, and End Results (SEER) registry from January 2000 to December 2012. Propensity score (PS) analysis with 1:2 matching was performed to ensure well-balanced characteristics of the comparison groups. Kaplan-Meier and Cox proportional hazardous model were used to evaluate the overall survival (OS), cancer-specific survival (CSS), the hazards ratio (HR) and corresponding 95% confidence intervals (95% CI). Results Overall, for all patients with stage IV HL, receiving radiotherapy was associated with both significantly improved OS and CSS. Radiotherapy to any lesions could independently improve the OS and CSS by 30% to 36% in the multivariate analyses before and after PS matching (PSM), with the best improvement of 33% to 40% observed for patients with nodular sclerosis ( < 0.05) among all HL pathological types. In particular, radiotherapy, most likely to the residual site, was more pronouncedly associated with the improvement in survival for patients with stage IV HL who were young (age<45, < .05) or without B symptoms for OS = 0.099, for CSS = 0.255). For those patients without B symptoms, after PSM, the OS was improved by 65% ( = .021). Conclusions The large SEER results support that radiotherapy is associated with better survival of patients with stage IV HL.
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http://dx.doi.org/10.1080/15384047.2020.1796194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537794PMC
September 2020

The key active degrader, metabolic pathway and microbial ecology of triclosan biodegradation in an anoxic/oxic system.

Bioresour Technol 2020 Dec 14;317:124014. Epub 2020 Aug 14.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.

A lab-scale anoxic/oxic (A/O) system was used to reveal the key active triclosan-degrading bacteria (TCS-DB) in this study. The results showed that TCS was mainly removed by metabolism of heterotrophic bacteria (accounting for about 62%), and the potential metabolic pathway was the break of ether bond in TCS formed 2,4-dichlorophenol, and further dechlorination formed phenol or other metabolic end products. DNA-based stable isotope probing (DNA-SIP) assay further revealed that Methylobacillus accounting for 20.75% in C sample was the key active TCS-DB. Furthermore, methylotrophy and methanol oxidation were found to be the potential metabolic routes of TCS degradation by functional annotation of prokaryotic taxa analysis. Interestingly, TCS accelerated the propagation of antibiotic resistance genes (fabI) and intI1 which positively correlated with several functional microorganisms (p < 0.05). This study contributes to comprehend the potential mechanism, metabolic pathway and microbial ecology of TCS biodegradation in A/O system.
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http://dx.doi.org/10.1016/j.biortech.2020.124014DOI Listing
December 2020

Effect of heat-inactivated N1115 on microbiota and gut-brain axis related molecules.

Biosci Microbiota Food Health 2020 19;39(3):89-99. Epub 2020 Feb 19.

Department of Nutrition, Food Safety and Toxicology, West China School of Public Health and West China Forth Hospital, and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, Sichuan, P. R. China.

This study was conducted to evaluate the possibility of using heated-inactivated lactobacilli to protect neonates from harmful effects of antibiotics. Thirty neonate mice were randomly divided into three groups of ten and treated with either sterilized water, an antibiotics cocktail, or the same antibiotics plus heat-inactivated N1115. The administration of antibiotics significantly increased the serum interleukin-6 (IL-6) levels of the tested mice (p<0.01, p<0.001, respectively) and decreased their serum corticosterone levels (p<0.01, p<0.01, respectively). The colonic crypts were significantly less deep in mice treated with antibiotics and with antibiotics plus N1115 (p<0.05). Antibiotics caused significantly abnormal expression of brain-derived neurotrophic factor (BDNF), γ-aminobutyric acid type A receptor α1 (GABA), γ-aminobutyric acid type B receptor1 (GABA), and 5-hydroxytryptamine receptor1A (5-HT) in the hippocampus (p<0.05, p<0.01, p<0.01, respectively) and of GABA in the prefrontal cortex (p<0.01). Heat-inactivated lactobacilli alleviated these abnormal changes. Antibiotics greatly decreased the Shannon index of the fecal microbiota and significantly increased the number of (p<0.001), with fewer and (p<0.05). Antibiotics not only cause microbiota dysbiosis, but also cause abnormal changes in important molecules in the gut-brain axis. All these abnormal changes are alleviated by heat-inactivated N1115. This indicates that heat-inactivated N1115 has a certain improvement effect on changes caused by antibiotics.
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http://dx.doi.org/10.12938/bmfh.2019-025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392912PMC
February 2020

Clinical and CT imaging features of SARS-CoV-2 patients presented with diarrhea.

J Infect 2020 12 5;81(6):e33-e35. Epub 2020 Aug 5.

Cancer Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2020.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405823PMC
December 2020

Ambiguous roles and potential therapeutic strategies of innate lymphoid cells in different types of tumor.

Oncol Lett 2020 Aug 16;20(2):1513-1525. Epub 2020 Jun 16.

Department of Thoracic Surgery, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.

Recent years have witnessed a significant development in the current understanding of innate lymphoid cells (ILCs) and their roles in the innate immune system, where they regulate tissue homeostasis, inflammation, as well as tumor surveillance and tumorigenesis. Based on the limited studies of ILCs in cancer, ILCs may be classified into three subgroups depending on their phenotypic and functional characteristics: Group 1 ILCs, which include natural killer cells and ILC1s; Group 2 ILCs, which only contain ILC2s and Group 3 ILCs, which comprise of LTi cells and ILC3s. Group 1 ILCs predominantly exert antitumor activities, while Group 2 ILCs and Group 3 ILCs are predominantly procarcinogenic in nature. In different types of tumor, each ILC subset behaves differently. Current research is focused on investigating how ILCs may be manipulated and employed as therapeutic strategies for the treatment of cancer. The present review aimed to summarize the characteristics and effects of ILCs in the context of tumor immunology, and provide novel insight into the pro- or anti-tumor activities of ILCs in different types of malignancy, including solid tumors, such as those in the gastrointestinal tract, lung, breast, bladder or prostate, as well as melanoma, further to hematological malignancies, with the aim to highlight potential therapeutic targets for the treatment of cancer.
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http://dx.doi.org/10.3892/ol.2020.11736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377136PMC
August 2020
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