Publications by authors named "Shijie Huang"

20 Publications

  • Page 1 of 1

Optimal Method of Electrical Stimulation for the Treatment of Upper Limb Dysfunction After Stroke: A Systematic Review and Bayesian Network Meta-Analysis of Randomized Controlled Trials.

Neuropsychiatr Dis Treat 2021 15;17:2937-2954. Epub 2021 Sep 15.

Department of Neurology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, People's Republic of China.

Background: The obstacle of limb motor caused by stroke, especially the decline of motor function of upper limbs, can directly affect the activities of daily living of stroke patients with hemiplegia. Based on long-term clinical practice, the treatment effect of electrical stimulation methods for stroke limb dysfunction has been widely recognized and supported by authoritative guidelines and systematic reviews. However, which electrical stimulation method is the optimum in the treatment of stroke limb dysfunction is still a controversial issue.

Objective: In this paper, we adopted Network Meta-Analysis (NMA) to rank the priorities of various electrical stimulation methods, so as to select the optimal electrical stimulation method and discuss its rationality in guiding clinical practice.

Methods: We carried out a systematic review by searching a total of 6806 studies from 8 databases and 2 clinical trial registries, and finally screened out 34 studies for further investigation. Then, pairwise meta-analysis and Bayesian network meta-analysis were employed to evaluate the effectiveness and ranking of various interventions. The primary outcome measure was Fugl-Meyer Assessment Upper Extremity (FMA-UE), and the secondary outcome measures were Modified Barthel Index (MBI) and Modified Ashworth Scale (MAS). Finally, the risk of bias, publication bias and sensitivity of the Randomized Controlled Trials (RCTs) were evaluated.

Results: On the basis of comprehensive rehabilitation treatment (RT), the Functional Electrical Stimulation (FES) was superior than other electrical stimulation methods in improving both FMA-UE and MBI. Meanwhile, the results indicated that the Transcutaneous Electrical Acupoint Stimulation (TEAS) was the only electrical stimulation method that showed treatment advantages in reducing MAS.

Conclusion: The study showed that FES had the optimal overall rehabilitation effect on upper limb dysfunction of stroke patients based on the comprehensive RT, while the treatment effect of TEAS on upper limb spasticity after stroke was the most significant.
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http://dx.doi.org/10.2147/NDT.S332967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450164PMC
September 2021

Exploring the effects of large-area dorsal skin irradiation on locomotor activity and plasm melatonin level in C3H/He mice.

Chronobiol Int 2021 Aug 5:1-10. Epub 2021 Aug 5.

Institute of Future Lighting, Academy for Engineering & Technology, Fudan University, Shanghai, China.

As the largest organ exposed to the outside of mammals, skin has direct photosensitivity. Recent studies have even shown that cutaneous irradiation played a role in local circadian systems. However, whether it can further affect the central clock system is controversial. Here, plasm melatonin rhythm of melatonin-proficient C3H/He mice was assessed, and on this basis, a well-designed segmented lighting method was used to investigate the effects of dorsal skin irradiation on locomotor activity and plasm melatonin content in male C3H/He mice. In brief, mice were separately exposed to cutaneous irradiation, intraocular irradiation or darkness for 60 min at specific moments. The results showed that neither blue nor red cutaneous exposure had obvious effect on central rhythm oscillation while intraocular irradiation could significantly change the central clock of mice, and the effect of blue light was more forceful than red light. It suggests that intraocular nonvisual channels still play a dominant role in rhythmic regulation, which has not been challenged by the discovery of local light entrainment in exposed peripheral tissues.
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http://dx.doi.org/10.1080/07420528.2021.1962904DOI Listing
August 2021

The review of the light parameters and mechanisms of Photobiomodulation on melanoma cells.

Photodermatol Photoimmunol Photomed 2021 Jun 28. Epub 2021 Jun 28.

Institute of Future Lighting, Academy for Engineering and Technology, Fudan University, Shanghai, China.

Photobiomodulation (PBM) uses low-intensity visible or near-infrared light to produce beneficial effects on cells or tissues, such as brain therapy, wound healing. Still there is no consistent recommendation on the parameters (dose, light mode, wavelength, irradiance) and protocols (repetition, treatment duration) for its clinical application. Herein, we summarize the current PBM parameters for the treatment of melanoma, and we also discuss the potential photoreceptors and downstream signaling mechanisms in the PBM treatment of melanoma cells. It is hypothesized that PBM may inhibit the melanoma cells by activating mitochondria, OPNs, and other receptors. Regardless of the underlying mechanisms, PBM has been shown to be beneficial in treating melanoma. Through further in-depth studies of the underlying potential mechanisms, it can strengthen the applications of PBM for the therapy of melanoma.
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http://dx.doi.org/10.1111/phpp.12715DOI Listing
June 2021

Thoracoscopic radical esophagectomy combined with left inferior pulmonary ligament lymphadenectomy for esophageal carcinoma via the right thoracic approach: A single-center retrospective study of 30 cases.

Medicine (Baltimore) 2021 Jun;100(23):e26302

Department of Cardiothoracic Surgery, The Affiliated Hospital of Putian University, Putian, Fujian.

Abstract: To evaluate the necessity, safety, and feasibility of left inferior pulmonary ligament lymphadenectomy during video-assisted thoracic surgery (VATS) radical esophagectomy via the right thoracic approach.Thirty patients (20 men, 10 women) with thoracic esophageal squamous cell carcinoma (ESCC) were recruited for this study. The patients' age ranged from 50 to 80 years, with an average age of 66.17 ± 7.47 years. After the patients underwent VATS radical esophagectomy and left inferior pulmonary ligament lymph node dissection (LIPLND) via the right thoracic approach, the operative outcomes included operative time, length of hospital stay, postoperative complications, number of lymph nodes removed, and postoperative pathologic results were evaluated.There were no massive hemorrhages of the left inferior pulmonary vein during the operation. The operative time of LIPLND was 8.67 ± 2.04 minutes, and the length of postoperative hospital stay was 12.23 ± 2.36 days. The postoperative complications included 2 cases of left pneumothorax, 4 pulmonary infection cases, and no chylothorax. Moreover, 68 LIPLNs were dissected, 5 of which were positive, and the degree of metastasis was 7.4%. The postoperative pathologic results showed that 3 cases of LIPLNs were positive, with a metastasis rate of 10.0%. Among them, 2 cases were SCC of the lower thoracic esophagus, and 1 case was SCC of the middle thoracic esophagus, which involved the lower segment.Thoracoscopic esophagectomy combined with left inferior pulmonary ligament lymphadenectomy for esophageal carcinoma via the right thoracic approach will not increase the difficulty of operation, increase the incidence of postoperative complications or prolong the postoperative hospital stay, and can theoretically reduce tumor recurrence. Therefore, we believe that LIPLND is necessary, safe, and feasible and is worthy of clinical popularization and application.
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http://dx.doi.org/10.1097/MD.0000000000026302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202552PMC
June 2021

Volatile DMNT directly protects plants against by disrupting the peritrophic matrix barrier in insect midgut.

Elife 2021 Feb 18;10. Epub 2021 Feb 18.

The National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, China.

Insect pests negatively affect crop quality and yield; identifying new methods to protect crops against insects therefore has important agricultural applications. Our analysis of transgenic plants showed that overexpression of , encoding the key biosynthetic enzyme for the natural plant product (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), led to a significant resistance against a major insect pest, . DMNT treatment severely damaged the peritrophic matrix (PM), a physical barrier isolating food and pathogens from the midgut wall cells. DMNT repressed the expression of in midgut cells, and knocking down resulted in PM rupture and death. A 16S RNA survey revealed that DMNT significantly disrupted midgut microbiota populations and that midgut microbes were essential for DMNT-induced killing. Therefore, we propose that the midgut microbiota assists DMNT in killing . These findings may provide a novel approach for plant protection against .
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http://dx.doi.org/10.7554/eLife.63938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924945PMC
February 2021

Ultra-Low Frequency Eccentric Pendulum-Based Electromagnetic Vibrational Energy Harvester.

Micromachines (Basel) 2020 Nov 16;11(11). Epub 2020 Nov 16.

MEMS Center, Harbin Institute of Technology, Harbin 150001, China.

With the development of low-power technology in electronic devices, the wireless sensor network shows great potential in applications in health tracing and ocean monitoring. These scenarios usually contain abundant low-frequency vibration energy, which can be collected through appropriate energy conversion architecture; thus, the common issue of limited battery life in wireless sensor devices could be solved. Traditional energy-converting structures such as the cantilever-beam type or spring-mass type have the problem of high working frequency. In this work, an eccentric pendulum-based electromagnetic vibration energy harvester is designed, analyzed, and verified with the finite element analysis method. The pendulum that contains alternative distributed magnets in the outer side works as a rotor and has the advantages of a simple structure and low center frequency. The structure size is well scalable, and the optimal output performance can be obtained by optimizing the coil thickness and width for a given diameter of the energy harvester. The simulation results show that the energy harvester could work in ultra-low frequencies of 0.2-3.0 Hz. A full-scale prototype of the energy harvester is manufactured and tested. The center working frequency is 2.0 Hz with an average output power of 8.37 mW, which has potential for application in driving low-power wireless sensor nodes.
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http://dx.doi.org/10.3390/mi11111009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696301PMC
November 2020

Ju Re Ba Du therapy for Postherpetic neuralgia: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Oct;99(44):e22992

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Introduction: Postherpetic neuralgia (PHN) is one of the most common types of chronic neuropathic pain, which seriously affects quality of the life because of pain severity and poor response to the currently available treatments. Ju Re Ba Du therapy as a form of acupuncture therapy which is proved to be effective in RCTs and very suitable for patients, has been used in Postherpetic neuralgia in patients for a long time, therefore a systematic review is necessary to provide available evidence for further study.

Methods And Analysis: The following databases will be searched from their inception to October 2020: Electronic database includes PubMed, Embase, Cochrane Library, Web of Science, Nature, Science online, VIP medicine information, and CNKI (China National Knowledge Infrastructure).

Primary Outcome: pain intensity assessed on a visual analogue scale (VAS); Additional outcomes:Data will be extracted by two researchers independently, risk of bias of the meta-analysis will be evaluated based on the Cochrane Handbook for Systematic Reviews of Interventions. All data analysis will be conducted by data statistics software Review Manager V.5.3. and Stata V.12.0.

Results: The results of this study will systematically evaluate the effectiveness and safety of Ju Re Ba Du therapy intervention for people with Postherpetic neuralgia.

Conclusion: The systematic review of this study will summarize the current published evidence of Ju Re Ba Du therapy for the treatment of Postherpetic neuralgia, which can further guide the promotion and application of it.

Ethics And Dissemination: This study is a systematic review, the outcomes are based on the published evidence, so examination and agreement by the ethics committee are not required in this study. We intend to publish the study results in a journal or conference presentations.

Osf Registration Number: September 29, 2020 osf.io/r6y9b. (https://osf.io/r6y9b).
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http://dx.doi.org/10.1097/MD.0000000000022992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598878PMC
October 2020

Safety and effectiveness of acupuncture for POSEIDON patients in IVF/ICSI: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Oct;99(42):e22768

Hospital of Chengdu University of Traditional Chinese Medicine, Sichuan, China.

Introduction: The purpose of this paper is to evaluate the efficacy and safety of acupuncture for POSEIDON patients undergoing IVF/ICSI.

Methods: and analysis We will electronically search Pubmed, Medline, Embase, Web of Science, the Cochrane Central Register of Controlled Trial, China National Knowledge Infrastructure, China Biomedical Literature Database, China Science Journal Database and Wan-fang Database from their inception. Also, we will manually retrieve other resources, including reference lists of identified publications, conference articles, and grey literature. The clinical randomized controlled trials or quasi randomized controlled trials related to acupuncture treatment for POSEIDON patients in IVF/ICSI will be included in the study. The language is limited to Chinese and English. Research selection, data extraction, and research quality assessment will be independently completed by two researchers. Data were synthesized by using a fixed effect model or random effect model depend on the heterogeneity test. The clinical pregnancy rate (CPR) and live birth rate (LBR) will be the primary outcomes. The ongoing pregnancy, miscarriage rate (MR) and adverse events will also be assessed as secondary outcomes. RevMan V.5.3 statistical software will be used for meta-analysis, and the level of evidence will be assessed by Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Continuous data will be expressed in the form of weighted mean difference or standardized mean difference with 95% confidence intervals (CIs), while dichotomous data will be expressed in the form of relative risk with 95% CIs.

Ethics And Dissemination: The protocol of this systematic review (SR) does not require ethical approval because it does not involve humans. We will publish this article in peer-reviewed journals and presented at relevant conferences.

Systematic Review Registration: OSF Registries, DOI: 10.17605/OSF.IO/6WP2F (https://osf.io/6wp2f).
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http://dx.doi.org/10.1097/MD.0000000000022768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571989PMC
October 2020

Identification of a genomic region controlling thermotolerance at flowering in maize using a combination of whole genomic re-sequencing and bulked segregant analysis.

Theor Appl Genet 2020 Oct 13;133(10):2797-2810. Epub 2020 Jun 13.

The National Engineering Laboratory of Crop Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.

Key Message: A novel genomic region controlling thermotolerance at flowering was identified by the combination of whole genomic re-sequencing and bulked segregant analysis in maize. The increasing frequency of extreme high temperature has brought a great threat to the development of maize throughout its life cycle, especially during the flowering phase. However, the genetic basis of thermotolerance at flowering in maize remains poorly understood. Here, we characterized a thermotolerant maize ecotype Abe2 and dissected its genetic basis using a F recombinant inbred line (RIL) population generated from a cross between Abe2 and B73. After continuous high temperature stress above 35 °C for 17 days, Abe2 and B73 show distinct leaf scorching phenotype under field conditions. To identify the genomic regions associated with the phenotypic variation, we applied a combination of whole genomic re-sequencing and bulked segregant analysis, and revealed 10,316,744 SNPs and 1,488,302 InDels between the two parental lines, and 2,693,054 SNPs and 313,757 InDels between the two DNA pools generated from the thermos-tolerant and the sensitive individuals of the RIL, of which, 108,655 and 17,853 SNPs may cause nonsynonymous variations. Finally, a 7.41 Mb genomic region on chromosome 1 was identified, and 7 candidate genes were annotated to participate in high temperature-related stress response. A candidate gene Zm00001d033339 encoding a serine/threonine protein kinase was proposed to be the most likely causative gene contributing to the thermotolerance at flowering by involving in stomatal movement (GO: 0010119) via Abscisic acid (ABA) pathway (KO04075). This work could provide an opportunity for gene cloning and pyramiding breeding to improve thermotolerance at flowering in maize.
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http://dx.doi.org/10.1007/s00122-020-03632-xDOI Listing
October 2020

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.

Nat Commun 2020 06 9;11(1):2900. Epub 2020 Jun 9.

Center for Biomolecular Sciences, University of Illinois at Chicago, Chicago, IL, 60607, USA.

5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell survival. By linking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain devoid of free 5S rRNA. Viability of the engineered cells demonstrates that autonomous 5S rRNA is dispensable for cell growth under standard conditions and is unlikely to have essential functions outside the ribosome. The fully assembled ribosomes carrying 23S-5S rRNA are highly active in translation. However, the engineered cells accumulate aberrant 50S subunits unable to form stable 70S ribosomes. Cryo-EM analysis revealed a malformed peptidyl transferase center in the misassembled 50S subunits. Our results argue that the autonomy of 5S rRNA is preserved due to its role in ribosome biogenesis.
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http://dx.doi.org/10.1038/s41467-020-16694-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283268PMC
June 2020

Method to allocate voting resources with unequal ballots and/or education.

MethodsX 2020 20;7:100872. Epub 2020 Mar 20.

Integrated Systems Engineering, The Ohio State University, Columbus, OH 43210, United States.

Apportionment in election systems refers to determination of the number of voting resources (poll books, poll workers, or voting machines) needed to ensure that all voters can expect to wait no longer than an appropriate amount, even the voter who waits the longest. Apportionment is a common problem for election officials and legislatures. A related problem is "allocation," which relates to the deployment of an existing number of resources so that the longest expected wait is held to an appropritate amount. Provisioning and allocation are difficult because the numbers of expected voters, the ballot lengths, and the education levels of voters may all differ significantly from precinct-to-precinct in a county. Consider that predicting the waiting time of the voter who waits the longest generally requires discrete event simulation.•The methods here rigorously guarantee that all voters expect to wait a prescribed time with a bounded probability, e.g., everyone expects to wait less than thirty minutes with probability greater than 95%.•The methods here can handle both a single type of resource (e.g., voting machines or scan machines) and multiple resource types (e.g., voting machines and poll books).•The methods are provided in a freely available, easy-to-use Excel software program.
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http://dx.doi.org/10.1016/j.mex.2020.100872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205765PMC
March 2020

The clinical value of a new method of functional lymph node dissection in video-assisted thoracic surgery right non-small cell lung cancer radical resection.

J Thorac Dis 2019 Feb;11(2):477-487

Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, China.

Background: To evaluate the safety, thoroughness and feasibility of "tunnel-type mediastinal lymph node dissection" in video-assisted thoracic surgery (VATS) for right non-small cell lung cancer (NSCLC) radical resection, which functionally dissected the lymph nodes of station 2R/4R/7.

Methods: A retrospective study was performed in the clinical data of 196 patients with VATS right NSCLC radical resection. According to the different methods of lymph node dissection of station 2R, 4R and 7, they were divided into the tunnel-type group (n=102) and the routine group (n=94). The clinical data of two group were compared.

Results: The analyses of the baselines of the two groups are comparable. For lymph nodes dissection of station 2R/4R/7, operation time, the total number, positive number and metastasis incidence shown no significant difference between two groups (P>0.05). However, the amount of bleeding, postoperative thoracic drainage volume, extubation time, hospitalization days, the incidence of postoperative pulmonary infection and chronic cough were significantly lower in the tunnel-type group (P<0.05). There was no significant difference in 3-year recurrence and metastasis and in 3-year survival between tunnel-type group and routine group.

Conclusions: The tunnel-type group has more advantages, such as less surgical trauma, shorter hospitalization time, faster postoperative rehabilitation, even less postoperative chronic cough compared with the routine group. Therefore, we believe that the tunnel-type mediastinal lymph node dissection is a safe, thorough and feasible surgical method, which is worthy of being popularized and applied in the VATS right NSCLC radical resection.
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http://dx.doi.org/10.21037/jtd.2019.01.15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409250PMC
February 2019

A useful method to keep azygos arch in minimally invasive resection of esophageal leiomyoma.

J Thorac Dis 2017 Nov;9(11):4589-4591

Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, China.

Lifting azygos arch with a prolene line is a useful way to keep azygos arch in minimally invasive resection of esophageal leiomyoma without affecting surgical exposure and operative procedure.
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http://dx.doi.org/10.21037/jtd.2017.09.152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721067PMC
November 2017

Simultaneous Determination of Furan and Vinyl Acetate in Vapor Phase of Mainstream Cigarette Smoke by GC-MS.

An Acad Bras Cienc 2017 May 4;89(1 Suppl 0):383-390. Epub 2017 May 4.

College of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan, China.

A simple and sensitive method for simultaneous determination of furan and vinyl acetate (VA) in vapor phase of mainstream cigarette smoke with cold trap and gas chromatography-mass spectrometry (GC-MS) was developed. A Cambridge filter pad (CFP) was placed in front of the impingers of smoking machine to remove the particle phase from cigarette smoke. Furan and VA in vapor phase of mainstream cigarette smoke were collected in two impingers connected in series by filled with methanol at -78°C. The solutions were added with deuterium-labeled furan-d4 and VA-d6 as internal standards and analyzed by GC-MS. The results showed that the calibration curves for furan and VA were linear (r2 > 0.9995) over the studied concentration range. The intra- and inter-day precision values for furan and VA were <7.07% and <9.62%, respectively. The extraction recoveries of furan and VA were in the range of 94.5-97.7% and 92.3-94.9%, respectively. Moreover, the limits of detection for furan and VA were 0.028 µg mL-1 and 1.3 ng mL-1, respectively. The validated method has been successfully applied to determine the emissions of furan and VA in the vapor phase of mainstream cigarette smoke under International Organization for Standardization (ISO) and Canadian Intense (CI) smoking regimen.
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http://dx.doi.org/10.1590/0001-3765201720160230DOI Listing
May 2017

Comparison of laparoscopic vs. open surgery for rectal cancer.

Mol Clin Oncol 2017 Feb 15;6(2):170-176. Epub 2016 Dec 15.

Department of Hepatobiliary Surgery, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

This study was conducted to evaluate the safety of laparoscopic radical resection for rectal cancer. A total of 64 cases of rectal cancer patients undergoing radical surgery between January, 1998 and March, 2010 were collected. The patients were divided into the laparoscopic rectal surgery group (LS group, n=31) and the open surgery group (OS group, n=33). Operation time, postoperative recovery, complications and tumor-free survival rate were compared between the two groups. The inclusion criteria were as follows: Standard Karnofsky score >70 prior to surgery, definitive pathological diagnosis and complete clinical data. The exclusion criteria were concomitant tumors affecting survival. With the Dixon operation, the LS group had a longer operation time compared with the OS group (271.2±56.2 vs. 216.0±62.7 min, respectively; P=0.036), and an earlier time of oral intake (3.0±0.9 vs. 4.7±1.0 days, respectively; P=0.000). There were no significant differences between the LS and OS groups in terms of intraoperative blood loss, number of lymph nodes retrieved, duration of postoperative hyperthermia and hospitalization time (P>0.05). With the Miles operation, there were no obvious differences between the LS and OS groups regarding operation time, intraoperative blood loss, number of lymph nodes retrieved, time of oral intake, duration of postoperative hyperthermia and hospitalization time (P>0.05). Furthermore, there were no significant differences between the LS and OS groups with the Dixon or Miles operation in terms of 3-year tumor-free survival rate (P>0.05). Thus, laparoscopic surgery appears to be a safe and feasible option for the treatment of rectal cancer.
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http://dx.doi.org/10.3892/mco.2016.1112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351748PMC
February 2017

Experience in clinical diagnosis and treatment of duodenal tumors.

Mol Clin Oncol 2016 Dec 25;5(6):731-739. Epub 2016 Oct 25.

Anatomical Institute of Minimally Invasive Surgery, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Small bowel tumors are rare tumors. Duodenal tumors occur more commonly compared with other small intestinal tumors. To summarize the clinicopathological features of duodenal tumors, in the present study 44 cases of duodenal tumors were collected, and the comparative clinicopathological characteristics between tumors of the ampulla and non-ampulla, the choice of treatment, and differences in the prognosis, were analyzed. The pathological type identified was predominantly adenocarcinoma; periampullary duodenal tumors were almost classifiable as adenocarcinoma in terms of their type. Non-ampulla duodenal tumors also included rare pathological types, such as stromal tumor and large B-cell lymphoma. The symptoms of duodenal tumors were non-specific, therefore rendering early diagnosis and treatment difficult. Due to jaundice, periampullary duodenal tumors were diagnosed earlier than non-ampulla duodenal tumors. Endoscopy and computed tomography (CT) examinations were valuable in terms of diagnosis, and were used as a means of screening. Carcinoembryonic antigen (CEA) and cancer antigen 199 (CA199) were revealed to be important as biomarkers. Radical surgery was the most effective treatment. Pancreaticoduodenectomy was revealed to be applicable in all cases of duodenal tumors. For non-ampulla duodenal tumors, partial duodenum resection and subtotal gastrectomy were appropriate for selection as methods of treatment. No survival benefits were identified for adjuvant chemotherapy. Duodenal tumors were shown to be a rare neoplasm with atypical symptoms; they should be diagnosed and treated as early as possible; CT and gastroscopy may be used for screening, radical surgery offers the best treatment; pancreatoduodenectomy is not the only surgery option available; and chemotherapy did not result in any survival benefits.
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http://dx.doi.org/10.3892/mco.2016.1061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228486PMC
December 2016

Video-assisted thoracoscopy the total mesoesophageal excision and systematic en bloc mediastinal lymph node dissection.

J Vis Surg 2016 30;2:102. Epub 2016 May 30.

Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou 350000, China.

A 59-year-old female presented with upper esophageal squamous cell carcinoma had swallowing disorders. We performed the total mesoesophageal excision (TME) and systematic en bloc mediastinal lymph node dissection via VATS. The surgery process was successful and the postoperative course was uneventful. A squamous cell carcinoma of stage T1aN0M0 was identified on pathological examination, and the postoperative examination of esophageal swallow diatrizoate meglumine and computed tomography (CT) scan confirmed no anastomosis fistula and no signs of recurrence.
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http://dx.doi.org/10.21037/jovs.2016.05.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783243PMC
May 2016

Detection and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter.

ACS Chem Biol 2016 Jan 4;11(1):31-7. Epub 2015 Nov 4.

Department of Chemistry and Chemical Biology, ‡Department of Biology, and §Center for Biomedical Engineering, University of New Mexico , Albuquerque, New Mexico 87131-0001, United States.

The ribosome is the quintessential antibacterial drug target, with many structurally and mechanistically distinct classes of antibacterial agents acting by inhibiting ribosome function. Detecting and quantifying ribosome inhibition by small molecules and investigating their binding modes and mechanisms of action are critical to antibacterial drug discovery and development efforts. To develop a ribosome inhibition assay that is operationally simple, yet provides direct information on the drug target and the mechanism of action, we have developed engineered E. coli strains harboring an orthogonal ribosome-controlled green fluorescent protein (GFP) reporter that produce fluorescent signal when the orthogonal ribosome is inhibited. As a proof of concept, we demonstrate that these strains, when coexpressing homogeneous populations of aminoglycoside resistant ribosomes, act as sensitive and quantitative detectors of ribosome inhibition by a set of 12 structurally diverse aminoglycoside antibiotics. We suggest that this strategy can be extended to quantifying ribosome inhibition by other drug classes.
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http://dx.doi.org/10.1021/acschembio.5b00779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765996PMC
January 2016

[Development of a rapid molecular typing method for Vibrio cholerae using melting curve-based multilocus melt typing].

Zhonghua Yu Fang Yi Xue Za Zhi 2015 Feb;49(2):122-7

Email:

Objective: To develop a high-throughput rapid method for Vibrio (V.) cholerae molecular typing based on Melting Curve-based Multilocus Melt Typing (McMLMT).

Methods: Seven housekeeping genes of V.cholerae were screened out, and for each gene, the specific primers were designed for correspondent genes as well as 4 probes covering polymorphism loci of sequences. After optimizing all parameters, a method of melting-curve analysis following asymmetric PCR was established with dual-fluorescent-reporter in two reaction tubes for each gene. A set of 28 Tm-values was obtained for each strain and then translated into a set of code of allelic genes, standing for the strain's McMLMT type (MT). Meanwhile, sequences of the 7-locus polymorphism were typed according to the method of MLST. To evaluate the efficiency and reliability of McMLMT, the data were compared with that of sequence-typing and PFGE using BioNumerics software.

Results: McMLMT method was established and refined for rapid typing of V. cholerae that a dozen of strains can be finished testing in a 3-hours PCR running using 96-well plates. 108 strains were analyzed and 28-Tm-values could be grouped and encoded according to 7 housekeeping gene to obtain the code set of allelic genes, and classified into 18 types (D = 0.723 3). Sequences of the 7 genes' polymorphism areas were directly clustered into the same 18 types with reference to MLST method. 46 of the strains, each represented a different PFGE type, could be classified into 13 types (D = 0.614 5) with McMLMT method and A- K groups at 85% similarity (D = 0.858 9) with PFGE method.

Conclusion: McMLMT method is a rapid high-throughput molecular typing method for batches of strains with a resolution equal to MLST method and comparable to PFGE group.
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February 2015

Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.

Appl Environ Microbiol 2009 Nov 18;75(22):6981-5. Epub 2009 Sep 18.

Department of Microbiology, Xiamen Centre for Disease Control and Prevention, Xiamen 361021, Fujian Province, China.

Vibrio cholerae is a natural inhabitant of the aquatic environment. However, its toxigenic strains can cause potentially life-threatening diarrhea. A quadruplex real-time PCR assay targeting four genes, the cholera toxin gene (ctxA), the hemolysin gene (hlyA), O1-specific rfb, and O139-specific rfb, was developed for detection and differentiation of O1, O139, and non-O1, non-O139 strains and for prediction of their toxigenic potential. The specificity of the assay was 100% when tested against 70 strains of V. cholerae and 31 strains of non-V. cholerae organisms. The analytical sensitivity for detection of toxigenic V. cholerae O1 and O139 was 2 CFU per reaction with cells from pure culture. When the assay was tested with inoculated water from bullfrog feeding ponds, 10 CFU/ml could reliably be detected after culture for 3 h. The assay was more sensitive than the immunochromatographic assay and culture method when tested against 89 bullfrog samples and 68 water samples from bullfrog feeding ponds. The applicability of this assay was confirmed in a case study involving 15 bullfrog samples, from which two mixtures of nontoxigenic O1 and toxigenic non-O1/non-O139 strains were detected and differentiated. These data indicate that the quadruplex real-time PCR assay can both rapidly and accurately detect/identify V. cholerae and reliably predict the toxigenic potential of strains detected.
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http://dx.doi.org/10.1128/AEM.00517-09DOI Listing
November 2009
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