Publications by authors named "Shi-jie Li"

102 Publications

Peri-implantation expression and regulation of ITGB8 in goat uterus.

Theriogenology 2021 Dec 18;180:130-136. Epub 2021 Dec 18.

The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Ruminants have a superficial implantation pattern. The extended conceptus attaches to the receptive endometrium to form the cotyledonary placenta. During the attachment, a large number of events occur at the maternal-fetal interface. However, the related molecular mechanisms have not been fully understood. Integrin beta8 (ITGB8) is a subunit of integrin beta involved in embryo implantation. In this study, we determined peri-implantation expression and regulation of ITGB8 in goat uterus. The mRNA and protein levels of ITGB8 were both high in goat endometrial luminal epithelium (LE) and superficial glandular epithelium (sGE) during the adhesion period (Days 16-19 of pregnancy). Such expression profile was opposite to that of microRNA-187 (miR-187). Then, we validated that miR-187 targeted the 3' untranslated region (UTR) of ITGB8 in primary goat endometrial epithelial cells (EECs). In EECs, inhibition of miR-187 resulted in not only up-regulated ITGB8 level but also reduced cell proliferation and focal adhesion kinase (FAK) activity. Moreover, ITGB8 and miR-187 were regulated by interferon tau (IFNT). Altogether, in goat, the miR-187/ITGB8 axis may be involved in conceptus attachment and is downstream of IFNT. Our results will help us better understand the mechanisms of ruminant implantation and may provide a useful tool to improve the reproduction ratio for ruminants.
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http://dx.doi.org/10.1016/j.theriogenology.2021.12.022DOI Listing
December 2021

A Facile Probe for Fluorescence Turn-on and Simultaneous Naked-Eyes Discrimination of HS and biothiols (Cys and GSH) and Its Application.

J Fluoresc 2022 Jan 23;32(1):175-188. Epub 2021 Oct 23.

Department of Chemistry, College of Art and Sciences, Northeast Agricultural University, Harbin, 150030, PR China.

Hydrogen sulfide and biothiol molecules such as Cys and GSH acted important roles in many physiological processes. To simultaneously detect and distinguish them was quite necessary by a suitable fluorescent probe. A novel chemosensor 4-(4-(benzo[d]thiazol-2-yl)-2-methoxyphenoxy)-7-nitrobenzo[c][1,2,5]oxadiazole (BMNO) was designed to detect HS/Cys/GSH using the combination of nitrobenzofurazan (NBD) and benzothiazole fluorophores linked by a facile ether bond. The probe BMNO was developed for simultaneous identification of HS, Cys and GSH. Noticeably, the color changes (from colorless to light purple, light orange and light yellow) of probe BMNO solutions for sensing HS, Cys and GSH could be observed by naked eyes, respectively. The probe BMNO exhibited high selectivity and sensitivity for HS, Cys and GSH showing distinct optical signal with detection limit as low as 0.15 μM, 0.03 μM and 0.14 μM, respectively. The sensing mechanism was clarified by spectrum analysis and some controlled experiments. In addition, these outstanding properties of probe BMNO enabled its practical applications in detection HS in beer, and in cell imaging for Cys and GSH as well.
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http://dx.doi.org/10.1007/s10895-021-02838-6DOI Listing
January 2022

Does Industrial Agglomeration or Foreign Direct Investment Matter for Environment Pollution of Public Health? Evidence From China.

Front Public Health 2021 19;9:711033. Epub 2021 Aug 19.

Academy of Financial Research, Business School, Wenzhou University, Wenzhou, China.

This article focuses on the interaction between China's industrial agglomeration, foreign direct investment (FDI) and environmental pollution of public health in the past 15 years. By conducting theoretical and empirical research, we try to reveal the relationship and mechanism between the economic growth and public health from the perspective of environmental pollution. By constructing an embedded theoretical model of industrial agglomeration and FDI, this article combines other environmental pollution influencing factors, expounds the impact mechanism of industrial agglomeration on environmental pollution. Based on the provincial-level panel data of China on environmental pollution and industrial agglomeration, the empirical test is carried out through the threshold panel regression model. According to the results, industrial agglomeration can significantly rectify the regional environmental pollution, thereby benefiting public health. FDI has a phased impact on the relationship between industrial agglomeration and environmental pollution. Specifically, when the level of FDI is low, the positive improvement effect of industrial agglomeration on environmental pollution is relatively strong. This is mainly because industrial agglomeration can promote economic growth, technological progress, and enhance environmental awareness. When the level of FDI exceeds the first threshold and continues to rise, the positive improvement effect of industrial agglomeration is maximized. Before the level of FDI exceeds the second threshold, this effect gradually weakens. The population concentration and excessive expansion of city scale brought about by industrial agglomeration will lead to the increase of regional resource and energy consumption, thus aggravating environmental pollution. The policy implication is that while the government and enterprises are vigorously increasing the level of foreign investment, they must pay equal attention to economic growth and public health, and the level of industrial agglomeration should match the level of foreign investment so as to give full play to the positive improvement effect of industrial agglomeration on environmental pollution, and realize the coordinated development of the regional economy, environment and population health.
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http://dx.doi.org/10.3389/fpubh.2021.711033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416622PMC
October 2021

Neutral medial cortical relation predicts a high loss rate of cortex support in pertrochanteric femur fractures treated by cephalomedullary nail.

Injury 2021 11 9;52(11):3530-3531. Epub 2021 Jul 9.

Department of Orthopaedic Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.injury.2021.07.002DOI Listing
November 2021

Artificial intelligence in gastroenterology and hepatology: Status and challenges.

World J Gastroenterol 2021 Apr;27(16):1664-1690

Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.

Originally proposed by John McCarthy in 1955, artificial intelligence (AI) has achieved a breakthrough and revolutionized the processing methods of clinical medicine with the increasing workloads of medical records and digital images. Doctors are paying attention to AI technologies for various diseases in the fields of gastroenterology and hepatology. This review will illustrate AI technology procedures for medical image analysis, including data processing, model establishment, and model validation. Furthermore, we will summarize AI applications in endoscopy, radiology, and pathology, such as detecting and evaluating lesions, facilitating treatment, and predicting treatment response and prognosis with excellent model performance. The current challenges for AI in clinical application include potential inherent bias in retrospective studies that requires larger samples for validation, ethics and legal concerns, and the incomprehensibility of the output results. Therefore, doctors and researchers should cooperate to address the current challenges and carry out further investigations to develop more accurate AI tools for improved clinical applications.
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http://dx.doi.org/10.3748/wjg.v27.i16.1664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072192PMC
April 2021

Chitooligosaccharides inhibit tumor progression and induce autophagy through the activation of the p53/mTOR pathway in osteosarcoma.

Carbohydr Polym 2021 Apr 21;258:117596. Epub 2021 Jan 21.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. Electronic address:

Osteosarcoma is the most common primary sarcoma of bone. The use of Chitooligosaccharide (COS) as a drug carrier is an emerging new strategy for cancer therapy. However, the application of COS in osteosarcoma has not been reported. Here, we investigated the influence of COS on osteosarcoma, and suggested the underlying mechanism. Initially, we obtained COS with a low-degree-polymerized (DP = 2-6) by enzymatic hydrolysis. Using these COS materials, in vitro assays showed that COS elicited the anti-tumor activity against osteosarcoma cells. We found that COS had significant effects on cell growth, metastasis inhibition, apoptosis and autophagy induction, and triggered pro-apoptosis autophagy through p53/mTOR signaling pathway in osteosarcoma cells. In addition, the COS also inhibited tumor growth and metastasis in an osteosarcoma xenograft model in vivo. Finally, we showed that COS could increase sensitivity to chemotherapy of cisplatin in vitro. Thus, we provide experimental evidence to demonstrate that COS has anti-tumor effect on osteosarcoma, and COS can be a new potential therapeutic candidate for the treatment of osteosarcoma.
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http://dx.doi.org/10.1016/j.carbpol.2020.117596DOI Listing
April 2021

Biliary stent combined with iodine-125 seed strand implantation in malignant obstructive jaundice.

World J Clin Cases 2021 Feb;9(4):801-811

Department of Interventional, Harbin Medical University Cancer Hospital, Harbin 150081, Heilongjiang Province, China.

Background: Malignant obstructive jaundice is mainly caused by cholangiocarcinoma. Only a few patients are indicated for surgical resection, and the 3-year survival rate is < 50%. For patients who are not eligible for surgery, biliary stent placement can relieve biliary obstruction and improve liver function and quality of life. However, restenosis after biliary stents has a poor prognosis and is a clinical challenge. Biliary stent combined with iodine-125 (I) seed implantation can prolong stent patency and improve survival.

Aim: To evaluate the safety and efficacy of biliary stent combined with I seed strand implantation in malignant obstructive jaundice.

Methods: We enrolled 67 patients between January 2016 and June 2018 with malignant obstructive jaundice and randomized them into a biliary stent combined with I seed strand treatment (combined) group 32) and biliary stent (control) group ( = 35). All patients underwent enhanced computed tomography and magnetic resonance imaging and were tested for biochemical and cancer markers. Twelve patients underwent pathological examination before surgery. All patients were followed up by telephone or clinical visit. Postoperative liver function improvement, postoperative complications, stent patency time, and survival time were compared between the two groups. Prognostic risk factors were evaluated.

Results: Technical success was achieved in all patients in both groups. Postoperative liver function improved significantly in all patients (total bilirubin, direct bilirubin, alanine aminotransferase, and aspartate aminotransferase decreased significantly in all patients, the values were less than 0.05). There was no significant difference in preoperative or postoperative indexes between the two groups for changes in total bilirubin ( = 0.147), direct bilirubin ( = 0.448), alanine aminotransferase ( = 0.120), and aspartate aminotransferase ( = 0.387) between the two groups. The median stent patency time of the combined group was 9.0 ± 1.4 mo [95% confidence interval (CI): 6.3-11.8 mo], which was significantly longer than the that of the control group (6.0 ± 0.3 mo, 95%CI: 5.5-6.5 mo, = 0.000). The median survival time of the combined group was 11.0 ± 1.4 mo (95%CI: 8.2-13.7 mo), which was significantly longer than that of the control group (7.0 ± 0.3 mo, 95%CI: 6.4-7.6 mo, = 0.000). Location of obstruction and number of stents were independent risk factors affecting prognosis.

Conclusion: Biliary stent combined with I seed strand implantation is safe and effective in malignant obstructive jaundice and improves stent patency time and median survival time.
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http://dx.doi.org/10.12998/wjcc.v9.i4.801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852634PMC
February 2021

Recyclable magnetic chitosan microspheres with good ability of removing cationic dyes from aqueous solutions.

Int J Biol Macromol 2021 Jan 10;167:1020-1029. Epub 2020 Nov 10.

National Engineering Research Center For Marine Aquaculture, Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhejiang Ocean University, Zhoushan 316022, China. Electronic address:

SrFeO-chitosan magnetic microparticles (SrFeO-CMNs) with a core-shell structure were synthesized, characterized and applied for the removal of two model cationic dyes. The results showed that these magnetic microparticles possess fast adsorption rate and high adsorption efficiency for both crystal violet (CV) and basic red 9 (BR9) at a temperature ranging 30 °C to 40 °C and suitable pH range (pH ≥ 7). The maximum removal efficiency for CV and BR9 attained to 94.5% and 97.5% in 30 min, which was significantly faster and higher than that of chitosan (<50% in 60 min) (P<0.01). And its maximum adsorption capacity for CV and BR9 reached 29.46 mg/g and 32.16 mg/g, respectively. The adsorption process of SrFeO-CMNs follows the Langmuir isotherm with a high correlation coefficient (R > 0.97) and the pseudo-second-order model. Additionally, the synthesized SrFeO-CMNs were easy to regeneration and reuse, and the removal rate remained above 90% after 5 recycle times. This study would provide a new more environmental friendly material and method for the treatment of wastewater containing toxic dyes.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.057DOI Listing
January 2021

MS-TCN++: Multi-Stage Temporal Convolutional Network for Action Segmentation.

IEEE Trans Pattern Anal Mach Intell 2020 Sep 4;PP. Epub 2020 Sep 4.

With the success of deep learning in classifying short trimmed videos, more attention has been focused on temporally segmenting and classifying activities in long untrimmed videos. State-of-the-art approaches for action segmentation utilize several layers of temporal convolution and temporal pooling. Despite the capabilities of these approaches in capturing temporal dependencies, their predictions suffer from over-segmentation errors. In this paper, we propose a multi-stage architecture for the temporal action segmentation task that overcomes the limitations of the previous approaches. The first stage generates an initial prediction that is refined by the next ones. In each stage we stack several layers of dilated temporal convolutions covering a large receptive field with few parameters. While this architecture already performs well, lower layers still suffer from a small receptive field. To address this limitation, we propose a dual dilated layer that combines both large and small receptive fields. We further decouple the design of the first stage from the refining stages to address the different requirements of these stages. Extensive evaluation shows the effectiveness of the proposed model in capturing long-range dependencies and recognizing action segments. Our models achieve state-of-the-art results on three datasets: 50Salads, Georgia Tech Egocentric Activities (GTEA), and the Breakfast dataset.
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http://dx.doi.org/10.1109/TPAMI.2020.3021756DOI Listing
September 2020

Blood donor recruitment in Guangzhou, China, during the 2019 novel coronavirus (COVID-19) epidemic.

Transfusion 2020 11 7;60(11):2597-2610. Epub 2020 Aug 7.

Guangzhou Blood Center, Guangzhou, Guangdong, China.

Background: The coronavirus disease 2019 (COVID-19) epidemic affected blood collection in Guangzhou, China.

Study Design And Methods: This paper includes three studies. The observational study reported the trends of blood collection during the epidemic in Guangzhou, China. The cross-sectional survey investigated factors influencing blood donation during the COVID-19 epidemic, and a self-administered questionnaire was given to 1584 street whole blood donors (SWBDs) who donated during the epidemic. The randomized controlled trial involved 19 491 SWBDs who donated in 2019 but did not donate during the epidemic. Trial participants were randomly assigned to two intervention groups: Group 1 completed Questionnaire 1, which contained precautionary measures in response to COVID-19 and other messages about blood donation during the epidemic; Group 2 completed Questionnaire 2, which did not include this information. A control group did not receive any questionnaire.

Results: As measures were implemented, the number of blood donors increased accordingly. Both first-time and repeat SWBDs perceived the same level of blood need and donated blood because it would save lives. SWBDs who completed Questionnaire 1 expressed a greater intention to donate during the epidemic. Enabling blood donors to perceive a higher level of blood need and a lower level of COVID-19 infection risk related to blood donation mobilized experienced SWBDs to donate within 3 weeks. Intention-to-treat analyses and average-treatment-effect-on-the-treated estimations confirmed that Questionnaire 1 could motivate SWBDs to actually donate blood.

Conclusion: Various measures could ease blood shortage during the COVID-19 epidemic. Administration of Questionnaire 1 could increase blood donations during the epidemic.
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http://dx.doi.org/10.1111/trf.15971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7361411PMC
November 2020

Laparoscopic surgery for early gallbladder carcinoma: A systematic review and meta-analysis.

World J Clin Cases 2020 Mar;8(6):1074-1086

Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University, Hangzhou 310016, Zhejiang Province, China.

Background: There is a controversy as to whether laparoscopic surgery leads to a poor prognosis compared to the open approach for early gallbladder carcinoma (GBC). We hypothesized that the laparoscopic approach is an alternative for early GBC.

Aim: To identify and evaluate the safety and feasibility of laparoscopic surgery in the treatment of early GBC.

Methods: A comprehensive search of online databases, including MEDLINE (PubMed), Cochrane libraries, and Web of Science, was performed to identify non-comparative studies reporting the outcomes of laparoscopic surgery and comparative studies involving laparoscopic surgery and open surgery in early GBC from January 2009 to October 2019. A fixed-effects meta-analysis was performed for 1- and 5-year overall survival and postoperative complications, while 3-year overall survival, operation time, blood loss, the number of lymph node dissected, and postoperative hospital stay were analyzed by random-effects models.

Results: The review identified 7 comparative studies and 8 non-comparative studies. 1068 patients (laparoscopic surgery: 613; open surgery: 455) were included in the meta-analysis of 1-, 3-, and 5-year overall survival with no significant differences observed [(HR = 0.54; 95%CI: 0.29-1.00; = 0.0%; = 0.051), (HR = 0.75; 95%CI: 0.34-1.65; = 60.7%; = 0.474), (HR = 0.71; 95%CI: 0.47-1.08; = 49.6%; = 0.107), respectively]. There were no significant differences in operation time [weighted mean difference (WMD) = 18.69; 95%CI: -19.98-57.36; = 81.4%; = 0.343], intraoperative blood loss (WMD = -169.14; 95%CI: -377.86-39.57; = 89.5%; = 0.112), the number of lymph nodes resected (WMD = 0.12; 95%CI: -2.95-3.18; = 73.4%; = 0.940), and the complication rate (OR = 0.69; 95%CI: 0.30-1.58; = 0.0%; = 0.377 ) between the two groups, while patients who underwent laparoscopic surgery had a reduced length of hospital stay (WMD = -5.09; 95%CI: -8.74- -1.45; = 91.0%; = 0.006).

Conclusion: This systematic review and meta-analysis confirms that laparoscopic surgery is a safe and feasible alternative to open surgery with comparable survival and operation-related outcomes for early GBC.
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http://dx.doi.org/10.12998/wjcc.v8.i6.1074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103978PMC
March 2020

Ablating atrial tachycardia from the right pulmonary artery: a case report.

Europace 2020 03;22(3):360

Heart Rhythm Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

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http://dx.doi.org/10.1093/europace/euz320DOI Listing
March 2020

A novel colorimetric and "turn-off" fluorescent probe based on catalyzed hydrolysis reaction for detection of Cu in real water and in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Feb 23;227:117540. Epub 2019 Oct 23.

Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

A novel and highly selective fluorescent 1,8-naphthalimide-based probe, 3, was designed and synthesized for rapid Cu detection in a CHCN-HO (3:1, v/v, pH = 7.4) solution by means of a distinct hydrolysis mechanism via its Cu-promoting feature. Upon treatment with Cu, the fluorescence response of probe 3 at 550 nm abruptly decreased, which was visible to the naked eye, and this response was accompanied by a clear change of the color of the solution; the color changed from the original yellow color to colorless. This color change occurred due to the Cu-promoted hydrolysis of 3, which yielded a fluorescence-quenched product. It is inspiring that probe 3 exhibited excellent sensitivity, a short response time and strong anti-interference recognition. Compared with the allowable amount of Cu (∼20 μM) in drinking water, the detection limit of 3 for Cu is calculated to be 9.15 nM, which is much lower than the amount defined by standards. The probe can be successfully applied for the determination of Cu in real aqueous samples. Furthermore, probe 3 can be used as a fluorescent sensor to detect Cu in biological environments, demonstrating its low toxicity to organisms and good cell permeability in live cell imaging.
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http://dx.doi.org/10.1016/j.saa.2019.117540DOI Listing
February 2020

Effectiveness of fibrin sealant as hemostatic technique in accelerating ESD-induced ulcer healing: a retrospective study.

Surg Endosc 2020 03 24;34(3):1191-1199. Epub 2019 Jun 24.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Surgery, Peking University Cancer Hospital and Institute, No. 52 Fu-Cheng Road, Haidian District, Beijing, 100142, China.

Objectives: Healing of gastric endoscopic submucosal dissection (ESD)-induced ulcer is critical for patient recovery. During ESD treatment, submucosal incisions are made with an electrosurgical knife to accomplish en bloc resections of superficial lesions. Nevertheless, excess electrocoagulation may decrease the blood supply of ESD-induced ulcer and delay the ulcer healing. The aim of this retrospective study was to evaluate the effectiveness of conservative electrocoagulation followed by porcine fibrin sealant (FS) as a wound microvessels-protective hemostatic technique in promoting the healing of ESD-induced ulcer.

Methods: A total of 332 patients with early gastric cancer (EGCs), or gastric precancerous lesion and gastric adenoma were retrospectively analyzed. Propensity score matching was used to compensate for the differences in age, gender, tumor location, resected specimen area, and pathology. One-month ulcer healing rates and delayed bleeding were compared between two matched groups (combined hemostats group and electrocautery group).

Results: A total of 115 matched pairs were created after propensity score matching. There was no difference in tumor location, specimen surface area, tumor differentiation and invasion depth between groups. The completed healing rate 1 month after ESD was 44.3% in combined hemostats group and 30.4% in electrocautery group (P = 0.004). There was no difference in delayed massive bleeding rate between two groups (P = 0.300). In addition, based on the multivariate regression analysis for ulcer healing rate, the use of FS (OR, 0.348, 95% CI 0.196 - 0.617, P = 0.000) and larger specimen size (OR, 2.640, 95% CI 2.015-3.458, P = 0.000) were associated with nonhealing ulcer 1 month after ESD.

Conclusion: Applying conservative electrocoagulation followed by porcine FS as a wound microvessels-protective hemostatic technique can promote ESD-induced ulcer healing without increasing delayed bleeding.
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http://dx.doi.org/10.1007/s00464-019-06872-1DOI Listing
March 2020

A lysosome-targetable fluorescent probe for imaging trivalent cations Fe, Al and Cr in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Nov 7;222:117242. Epub 2019 Jun 7.

Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China; College of Life Science, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

An effective morpholine-type naphthalimide chemsensor, N-p-chlorophenyl-4-(2-aminoethyl)morpholine-1,8-naphthalimide (CMN) has been developed as a lysosome-targeted fluorometric sensor for trivalent metal ions (Fe, Al and Cr). Upon the addition of Fe, Al or Cr ions, the probe CMN showed an evident naked-eye color changes which pale yellow solution of CMN turned deepened and it displayed turn-on fluorescence response in methanol. CMN showed a significant selective and sensitive toward Fe, Al or Cr ions, while there was no obvious behavior to other monovalent or divalent metal ions from the UV-vis and fluorescence spectrum. Based on the Job's plot analyses the 1:1 coordination mode of CMN with Fe, Al or Cr was proposed. The limit of detection (LOD) observed were 0.65, 0.69 and 0.68 μM for Fe, Al and Cr ions, respectively. The N-atom of morpholine directly involved in complex formation, CMN emitted fluorescence through inhibition of photoinduced electron transfer (PET). This probe exhibited excellent imaging ability for Fe, Aland Cr ions in living cells with low cytotoxicity. Significantly, the cellular confocal microscopic research indicated that the lysosome-targeted group of morpholine moiety was introduced which realized the capability of imaging lysosomal trivalent metal ions in living cells for the first time.
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http://dx.doi.org/10.1016/j.saa.2019.117242DOI Listing
November 2019

Postoperative acute kidney failure and incision skin necrosis caused by a giant retroperitoneal paraganglioma.

Chin Med J (Engl) 2019 Mar;132(6):733-734

Department of Hepatobiliary Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine & Zhejiang Chinese Medical University Affiliated Hangzhou First Hospital, Hangzhou, Zhejiang 310006, China.

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http://dx.doi.org/10.1097/CM9.0000000000000113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416021PMC
March 2019

Splenosis of douglas fossa 15 years after traumatic splenectomy mimicking pelvic masses.

Chin Med J (Engl) 2019 Jan;132(2):245-246

Department of Hepatobiliary Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China.

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http://dx.doi.org/10.1097/CM9.0000000000000075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365264PMC
January 2019

FoxO1 enhances differentiation and apoptosis in human primary keratinocytes.

Exp Dermatol 2018 11 26;27(11):1254-1260. Epub 2018 Sep 26.

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Forkhead box-O1 (FoxO1) is a key nutrient- and growth factor-dependent regulator of metabolism, but its functional role in human primary keratinocytes (HPKs) is less known. To investigate the role of FoxO1 in HPKs and effect of insulin-like growth factor 1 (IGF-1) and isotretinoin on FoxO1 expression, HPKs were treated with 1.2 mmol/L calcium chloride, 1-20 ng/mL IGF-1 and 0.1-10 μmol/L isotretinoin. Recombinant adenovirus expressing FoxO1 or FKHR shRNA lentivirus transfection was introduced to upregulate or silence FoxO1 expression. Epidermal FoxO1 immunostaining was lower in acne lesion than in normal skin. FoxO1 overexpression induced involucrin expression, G2/M arrest and apoptosis but suppressed proliferation, while FoxO1 silencing decreased involucrin expression but increased proliferation, S phase and viable cells in HPKs. IGF-1 downregulated FoxO1 and involucrin but upregulated p-Akt expression in HPKs, which was blocked by pretreatment with LY294002. Isotretinoin enhanced FoxO1, p53 and p21 but inhibited p-FoxO1 and involucrin expression in HPKs. These results demonstrate that FoxO1 promotes differentiation and apoptosis in HPKs. IGF-1 may reduce keratinocyte differentiation through PI3K/Akt/FoxO1 pathway, while isotretinoin can reinforce FoxO1 expression. FoxO1 may be involved in acne pathogenesis and could serve as a potential therapeutic target.
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http://dx.doi.org/10.1111/exd.13775DOI Listing
November 2018

A naphthalene-quinoline based chemosensor for fluorescent "turn-on" and absorbance-ratiometric detection of Al and its application in cells imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 20;205:276-286. Epub 2018 Jul 20.

School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, PR China.

A new naphthalene-quinoline based chemosensor L was prepared and structurally characterized. L exhibited excellent selectivity and sensitivity to Al through distinct fluorescence enhancement (335-fold) and ratiometric detection in DMF/HO (v/v, 1/9) based on the combined mechanisms of ESIPT and CHEF. The recognizing behavior of L toward Al had been investigated in detail through Job's Plot, FT-IR, HNMR, and HRMS analysis. The limit of detection (LOD) for Al was as low as and 3.67 × 10 M. L was successfully applied in real sample detection and construction of molecular logic gate. Moreover, L was verified to be of low cytotoxicity and good imaging characteristics for the detection of Al in cells HSC.
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http://dx.doi.org/10.1016/j.saa.2018.07.039DOI Listing
December 2018

Ultrastrukturelle Betrachtung bei einem Fall eines muzinösen Naevus.

J Dtsch Dermatol Ges 2018 Jun;16(6):778-780

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

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http://dx.doi.org/10.1111/ddg.13528_gDOI Listing
June 2018

Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes.

Biomed Res Int 2018 5;2018:7174561. Epub 2018 Apr 5.

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.
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http://dx.doi.org/10.1155/2018/7174561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907408PMC
October 2018

Ultrastructural observation in a case of mucinous nevus.

J Dtsch Dermatol Ges 2018 Jun 15;16(6):778-780. Epub 2018 May 15.

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

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http://dx.doi.org/10.1111/ddg.13528DOI Listing
June 2018

Protective roles and mechanisms of polysaccharides from Dendrobium officinal on natural aging-induced premature ovarian failure.

Biomed Pharmacother 2018 May 22;101:953-960. Epub 2018 Mar 22.

Guangzhou University of Chinese Medicine, Mathematical of Engineering Academy of Chinese Medicine, Guangzhou, 510006, PR China; Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou, 510006, PR China. Electronic address:

This study was designed to investigate the pharmacological effects and mechanisms of polysaccharides from Dendrobium officinal (DOP) on premature ovarian failure (POF) in natural aging mice. Fifteen months old female mice (n  =  28) and young adult female mice (n  =  14, 6 weeks) were used. DOP (70 mg/kg) was administrated to mice by oral gavage for 10 weeks and the protection effects of DOP on ovaries were investigated in vivo. The results showed that DOP reduced body weight, ovary and uterus/body weight parameters to normal level and alleviated ovarian pathological damage. Moreover, DOP could reduce pro-inflammatory cytokines (TNF-α, IL-6) and MDA levels and improve estradiol, SOD, GSH-Px, T-AOC and IL-10 levels in serum. These results suggested that DOP may alleviate the damage caused by aging through the inhibition of the nuclear factor -κB (NF-κB) and p53/Bcl-2-mediate signaling pathways. Moreover, we found that DOP can increase the numbers of mitochondria and endoplasmic reticulum. Moreover, DOP increased the numbers of different stages of follicular cells and improved mitochondrial membrane potential in ovaries. These results indicated that DOP may relieve ovarian damage through the protection of mitochondria in the ovaries. These findings suggest that DOP may be a promising drug for treating POF caused by natural aging in females.
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http://dx.doi.org/10.1016/j.biopha.2018.03.030DOI Listing
May 2018

Three kinds of lateral flow immunochromatographic assays based on the use of nanoparticle labels for fluorometric determination of zearalenone.

Mikrochim Acta 2018 03 24;185(4):238. Epub 2018 Mar 24.

Medical College Nankai University, Tianjin, 300500, China.

Colloidal gold, quantum dots and polystyrene microspheres were used as labels in three kinds of lateral flow immunochromatographic assays (ICAs) for the detection of zearalenone (ZEN) in cereal samples. The assays allow ZEN to be quantified within 20 min. The LODs are 10 μg·L of ZEN for the colloidal gold-based ICA, and 1 μg·L for both the quantum dot and polystyrene microsphere based ICAs. The respective data are 60 μg·kg, 6 μg·kg and 6 μg·kg, respectively, for spiked samples and cereals. Only minor cross-sensitivity occurred between ZEN and fusarium toxins, and no cross-sensitivity if found for aflatoxin B, T-2 mycotoxin, ochratoxin A, deoxynivalenol, and fumonisin B. LODs of the three assays are lower than the maximum limits of ZEN set by most standardization agencies. Graphical abstract Schematic presentation of three lateral flow immunochromatographic assays (ICAs) based on the use of (a) colloidal gold (CG), (b) fluorescent quantum dots (QD), and
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http://dx.doi.org/10.1007/s00604-018-2778-6DOI Listing
March 2018

EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.

J Exp Clin Cancer Res 2018 Mar 6;37(1):50. Epub 2018 Mar 6.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600, Yishan Road, Shanghai, 200233, China.

Background: Dysregulation of eukaryotic translation elongation factor 1 delta (EEF1D) in cancers has been reported; however, the role and mechanisms of EEF1D in osteosarcoma remain poorly understood. The aim of this study is to investigate the expression and role of EEF1D in osteosarcoma and to elucidate its underlying mechanisms.

Methods: The expression of EEF1D in osteosarcomas and cell lines was evaluated by qRT-PCR, Western blotting and immunohistochemistry. EEF1D knockdown using small interfering RNA (siRNA) was employed to analyze the role of EEF1D in osteosarcoma cell proliferation and cell cycle progression. The host signaling pathways affected by EEF1D knockdown were detected using PathScan® intracellular signaling array kit.

Results: The expression of EEF1D was found to be up-regulated in human osteosarcoma tissues and cell lines. Its expression was positively correlated with Enneking stage and the tumor recurrence. EEF1D knockdown inhibited osteosarcoma cell proliferation, colony-forming ability, and cell cycle G2/M transition in vitro. In addition, EEF1D knockdown decreased the levels of phospho-Akt, phospho-mTOR, and phospho-Bad proteins.

Conclusions: EEF1D is upregulated in osteosarcoma and plays a tumor promoting role by facilitating Akt-mTOR and Akt-Bad signaling pathways. Accordingly, EEF1D is a potential target for cancer therapy.
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http://dx.doi.org/10.1186/s13046-018-0715-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839064PMC
March 2018

Nevus anelasticus in a school-aged girl: ultrastructural observation and Er:YAG laser treatment.

Eur J Dermatol 2018 02;28(1):125-126

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

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http://dx.doi.org/10.1684/ejd.2017.3191DOI Listing
February 2018

Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway.

J Cancer 2017 22;8(14):2720-2728. Epub 2017 Aug 22.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

: Osteosarcoma is the most common malignant bone tumor in adolescents; however, the mechanisms involved in the pathogenesis and progression of osteosarcoma remain to be elucidated. Researchers have provided valuable insights into the tumorigenesis of Ribosomal protein S9 (RPS9) in some cancers. The purpose of this study was to elucidate the expression, functions, and mechanisms of RPS9 in human osteosarcoma. : The expression of RPS9 in osteosarcoma tissues and cell lines was evaluated by qRT-PCR and western blotting. Knockdown of RPS9 induced by RNA interference (RNAi) method in three osteosarcoma cell lines (MNNG/HOS, MG63, and U2OS) was employed to analyze the effects of RPS9 on cell proliferation and cell cycle distribution. The host signaling pathways affected by RPS9 were detected using the intracellular signaling antibody array kit PathScan. : The expression of RPS9 was found to be up-regulated in human osteosarcoma tissues and cell lines. Its expression was positively correlated with Enneking stage and the tumor recurrence. Down-regulation of RPS9 inhibited osteosarcoma cell proliferation, colony-forming ability, and cell cycle G1 phase in vitro. In addition, our data demonstrated that knockdown of RPS9 repressed the protein levels of phospho-SAPK/JNK and phospho-p38. : RPS9 is up-regulated and has a pro-tumor effect in osteosarcoma through the activation of MAPK signaling pathway and thus can be used as a potential target for gene therapy.
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http://dx.doi.org/10.7150/jca.19130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604204PMC
August 2017

Downregulation of BZW2 inhibits osteosarcoma cell growth by inactivating the Akt/mTOR signaling pathway.

Oncol Rep 2017 Oct 8;38(4):2116-2122. Epub 2017 Aug 8.

Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.

Osteosarcoma is the most common malignant bone tumor in adolescents. The function of basic leucine zipper and W2 domains 2 (BZW2) in tumor progression has been reported. However, the role and mechanisms of BZW2 in osteosarcoma remain to be determined. The aim of the present study was to reveal the expression and biological functions of BZW2 in osteosarcoma and to elucidate the proximal mechanisms underlying these functions. The expression of BZW2 in osteosarcoma tissues and cell lines was assessed by qRT-PCR, western blotting and immunohistochemistry. BZW2 overexpression was detected in osteosarcoma cell lines. Clinically, BZW2 expression was higher in osteosarcoma tissues than in corresponding non-tumor tissues and was associated with advanced Enneking stage and tumor recurrence. The knockdown of BZW2 using siRNA inhibited osteosarcoma cell proliferation, colony-forming ability, and the cell cycle at the G2/M phase in vitro. Host signaling pathways affected by BZW2 were detected using a PathScan Intracellular Signaling Antibody Array kit. These data demonstrated that the knockdown of BZW2 suppresses protein phosphorylation in the Akt/mTOR signaling pathway. These observations suggest that BZW2 is upregulated and has a pro-tumor effect in osteosarcoma via activation of the Akt/mTOR signaling pathway and thus is a potential target for gene therapy.
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http://dx.doi.org/10.3892/or.2017.5890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652953PMC
October 2017

Minichromosome maintenance protein 2 and 3 promote osteosarcoma progression via DHX9 and predict poor patient prognosis.

Oncotarget 2017 Apr;8(16):26380-26393

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

A label free quantitative proteomic approach (SWATH™ experiment) was performed to identify tumor-associated nuclear proteins that are differentially expressed between osteosarcoma cells and osteoblast cells. By functional screening, minichromosome maintenance protein 2 (MCM2) and minichromosome maintenance protein 3 (MCM3) were found to be related to osteosarcoma cell growth. Here, we show that knockdown of MCM2 or MCM3 inhibits osteosarcoma growth in vitro and in vivo. In co-immunoprecipitation and co-localization experiments, MCM2 and MCM3 were found to interact with DExH-box helicase 9 (DHX9) in osteosarcoma cells. A rescue study showed that the decreased growth of osteosarcoma cells by MCM2 or MCM3 knockdown was reversed by DHX9 overexpression, indicating that MCM2 and MCM3 activity was DHX9-dependent. In addition, the depletion of DHX9 hindered osteosarcoma cell proliferation. Notably, MCM2 and MCM3 expression levels were positively correlated with the DHX9 expression level in tumor samples and were associated with a poor prognosis in patients with osteosarcoma. Taken together, these results suggest that the MCM2/MCM3-DHX9 axis has an important role in osteosarcoma progression.
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http://dx.doi.org/10.18632/oncotarget.15474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432265PMC
April 2017

Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Apoptosis of Gastric Cancer Cells via PTEN/p-AKT Pathway.

Dig Dis Sci 2017 08 27;62(8):2004-2010. Epub 2017 Apr 27.

Department of General Surgery, Huaihe Hospital, Henan University, No. 1 Baobei Rd., Kaifeng, 475000, Henan, China.

Background: Long noncoding RNA (lncRNA) plays critical roles in both tumor-suppressive and oncogenic pathways in the pathological development and prognosis of cancers.

Aims: This study aimed to explore the expression of lncRNA AFAP1-AS1 and its function in gastric cancer (GC).

Methods: The expression of AFAP1-AS1 was detected in GC tissues and GC cells by quantitative real-time reverse-transcription PCR. A small interfering RNA (siRNA) that targeted AFAP1-AS1 was transfected into cells to inhibit the expression of AFAP1-AS1. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and colony formation assay were performed to examine the cell proliferation of SGC7901 cell transfected with si-AFAP1-AS1. Cell apoptosis was detected by flow cytometry. The protein level of cleaved PARP, Caspase 3, Caspase 9, Caspase 8, Bcl-2, Bax, p-AKT, total-AKT, and PTEN were detected by Western blot.

Results: AFAP1-AS1 was up-regulated in GC tissues and GC cells. AFAP1-AS1 knockdown suppressed cell viability of SGC7901 transfected with si-AFAP1-AS1. The number of apoptotic SGC7901 cell transfected with si-AFAP1-AS1 was increased by 3.4-fold comparing to that of control. The protein level of cleaved PARP, Caspase 3, and Caspase 9 were increased in SGC7901 transfected with si-AFAP1-AS1, as well as the expression of Bax. The protein level of Bcl-2 was decreased. AFAP1-AS1 knockdown decreased the protein level of p-AKT and increased the expression of PTEN in SGC7901 cells.

Conclusions: AFAP1-AS1 was up-regulated in GC cells and regulated the gastric cancer cell proliferation and apoptosis via PTEN/p-AKT pathway.
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http://dx.doi.org/10.1007/s10620-017-4584-0DOI Listing
August 2017
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