Publications by authors named "Sheng Wang"

1,608 Publications

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An Immune-Related Gene Pairs Signature for Predicting Survival in Glioblastoma.

Authors:
Sheng Wang Xia Xu

Front Oncol 2021 30;11:564960. Epub 2021 Mar 30.

Department of General Medicine, Xiangya Hospital, Central South University, Changsha, China.

Glioblastoma (GBM) is the frequently occurring and most aggressive form of brain tumors. In the study, we constructed an immune-related gene pairs (IRGPs) signature to predict overall survival (OS) in patients with GBM. We established IRGPs with immune-related gene (IRG) matrix from The Cancer Genome Atlas (TCGA) database (Training cohort). After screened by the univariate regression analysis and least absolute shrinkage and selection operator (LASSO) regression analysis, IRGPs were subjected to the multivariable Cox regression to develop an IRGP signature. Then, the predicting accuracy of the signature was assessed with the area under the receiver operating characteristic curve (AUC) and validated the result using the Chinese Glioma Genome Atlas (CGGA) database (Validation cohorts 1 and 2). A 10-IRGP signature was established for predicting the OS of patients with GBM. The AUC for predicting 1-, 3-, and 5-year OS in Training cohort was 0.801, 0.901, and 0.964, respectively, in line with the AUC of Validation cohorts 1 and 2 [Validation cohort 1 (1 year: 0.763; 3 years: 0.786; and 5 years: 0.884); Validation cohort 2 (1 year: 0.745; 3 years: 0.989; and 5 years: 0.987)]. Moreover, survival analysis in three cohorts suggested that patients with low-risk GBM had better clinical outcomes than patients with high-risk GBM. The univariate and multivariable Cox regression demonstrated that the IRGPs signature was an independent prognostic factor. We developed a novel IRGPs signature for predicting OS in patients with GBM.
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http://dx.doi.org/10.3389/fonc.2021.564960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042321PMC
March 2021

Girdin Knockdown Increases Gemcitabine Chemosensitivity to Pancreatic Cancer by Modulating Autophagy.

Front Oncol 2021 29;11:618764. Epub 2021 Mar 29.

Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Chemotherapy is crucial for the treatment of pancreatic cancer (PC). Gemcitabine (GEM) as the first-line chemotherapy drug has a high resistance rate. Increasing the sensitivity of gemcitabine is currently the objectives and challenges of this study. Our previous study showed Girdin was closely related to the progression and prognosis of PC, indicating that Girdin may be associated with chemosensitivity. In the current study, we use recombinant adenovirus to specifically knockdown Girdin in PC cell lines to determine the effect of Girdin in the process of gemcitabine chemosensitivity. Autophagy is one of the pathways affecting the gemcitabine chemosensitivity in PC. Further research validated that Girdin may activate autophagy by interacting with autophagy protein p62/SQSTM1, which could enhance chemotherapy resistance to gemcitabine in PC. Down-regulation of Girdin may therefore increase gemcitabine chemosensitivity in PC. Our results reveal that Girdin acted as a negative regulator of gemcitabine chemosensitivity in PC. Increased autophagy activity caused by abnormally high Girdin expression may be one of the main factors for the reduction in chemosensitivity, which may provide new perspectives on understanding chemosensitization in PC.
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http://dx.doi.org/10.3389/fonc.2021.618764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039524PMC
March 2021

SPL36 Encodes a Receptor-like Protein Kinase that Regulates Programmed Cell Death and Defense Responses in Rice.

Rice (N Y) 2021 Apr 7;14(1):34. Epub 2021 Apr 7.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.

Lesion mimic mutants spontaneously produce disease spots in the absence of biotic or abiotic stresses. Analyzing lesion mimic mutants' sheds light on the mechanisms underlying programmed cell death and defense-related responses in plants. Here, we isolated and characterized the rice (Oryza sativa) spotted leaf 36 (spl36) mutant, which was identified from an ethyl methanesulfonate-mutagenized japonica cultivar Yundao population. spl36 displayed spontaneous cell death and enhanced resistance to rice bacterial pathogens. Gene expression analysis suggested that spl36 functions in the disease response by upregulating the expression of defense-related genes. Physiological and biochemical experiments indicated that more cell death occurred in spl36 than the wild type and that plant growth and development were affected in this mutant. We isolated SPL36 by map-based cloning. A single base substitution was detected in spl36, which results in a cysteine-to-arginine substitution in SPL36. SPL36 is predicted to encode a receptor-like protein kinase containing leucine-rich domains that may be involved in stress responses in rice. spl36 was more sensitive to salt stress than the wild type, suggesting that SPL36 also negatively regulates the salt-stress response. These findings suggest that SPL36 regulates the disease resistance response in rice by affecting the expression of defense- and stress-related genes.
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http://dx.doi.org/10.1186/s12284-021-00475-yDOI Listing
April 2021

Spectral control of terahertz radiation from inhomogeneous plasma filaments by tailoring two-color laser beams.

Opt Express 2021 Mar;29(6):8676-8684

Terahertz (THz) radiation from an inhomogeneous plasma filament generated by focusing two-color femtosecond laser pulses into argon gas filled in a chamber is investigated experimentally by tailoring the Gaussian pump laser beams with an iris, where broadband THz emission over 10 THz is produced. It is found that the collected far-field THz radiation includes not only coherent but also partial-coherent components of the THz waves, which are emitted from the different parts of the inhomogeneous plasma filament with different plasma densities, contributing correspondingly to the different frequencies of the THz spectrum. Our results suggest that the THz spectrum can be manipulated by controlling the plasma density distribution of the filaments.
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http://dx.doi.org/10.1364/OE.417515DOI Listing
March 2021

Structural and Secondary Electron Yield Properties of Titanium-Palladium Films with Laser-Treated Copper Substrate for Application in Neutron Generators.

Materials (Basel) 2021 Mar 5;14(5). Epub 2021 Mar 5.

Shaanxi Engineering Research Center of Advanced Nuclear Energy & Shaanxi Key Laboratory of Advanced Nuclear Energy and Technology & School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Secondary electron emission (SEE) of the oxygen-free high-conductivity copper (OFHC) target surface in neutron generators limits the stability and improvement of the neutron yield. A novel-type target of titanium-palladium films coated on laser-treated OFHC target substrate was proposed and explored in this work to obtain low secondary electron yield (SEY) without introducing any components. The combination of Ti-Pd films and laser-treated OFHC substrate can effectively suppress secondary electron emission and enhance the adsorption ability to hydrogen isotopes with the existence of Pd film. The surface morphologies, surface chemical states, and SEYs of Ti-Pd films with laser-treated OFHC substrate were studied systematically for the first time. The XPS results showed that the laser-treated OFHC substrate surface was basically covered by Pd film. However, the Pd film surface was partially oxidized, with percentages of 21.31 and 10.02% for PdO and PdO, respectively. The SEYs of Ti-Pd films with laser-treated OFHC substrate were all below 1 within the investigated primary energy range of 100-3000 eV, which would be sufficient for application in neutron generators. Specifically, the maximum SEY () of laser-treated OFHC substrate coated by Ti-Pd films was 0.87 with corresponding incident electron energy of 400 eV.
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http://dx.doi.org/10.3390/ma14051222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961749PMC
March 2021

Opportunities and challenges for the computational interpretation of rare variation in clinically important genes.

Am J Hum Genet 2021 Apr;108(4):535-548

Departments of Bioengineering & Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address:

Genome sequencing is enabling precision medicine-tailoring treatment to the unique constellation of variants in an individual's genome. The impact of recurrent pathogenic variants is often understood, however there is a long tail of rare genetic variants that are uncharacterized. The problem of uncharacterized rare variation is especially acute when it occurs in genes of known clinical importance with functionally consequential variants and associated mechanisms. Variants of uncertain significance (VUSs) in these genes are discovered at a rate that outpaces current ability to classify them with databases of previous cases, experimental evaluation, and computational predictors. Clinicians are thus left without guidance about the significance of variants that may have actionable consequences. Computational prediction of the impact of rare genetic variation is increasingly becoming an important capability. In this paper, we review the technical and ethical challenges of interpreting the function of rare variants in two settings: inborn errors of metabolism in newborns and pharmacogenomics. We propose a framework for a genomic learning healthcare system with an initial focus on early-onset treatable disease in newborns and actionable pharmacogenomics. We argue that (1) a genomic learning healthcare system must allow for continuous collection and assessment of rare variants, (2) emerging machine learning methods will enable algorithms to predict the clinical impact of rare variants on protein function, and (3) ethical considerations must inform the construction and deployment of all rare-variation triage strategies, particularly with respect to health disparities arising from unbalanced ancestry representation.
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http://dx.doi.org/10.1016/j.ajhg.2021.03.003DOI Listing
April 2021

All-Inorganic Quantum Dot Light-Emitting Diodes with Suppressed Luminance Quenching Enabled by Chloride Passivated Tungsten Phosphate Hole Transport Layers.

Small 2021 Mar 30:e2100030. Epub 2021 Mar 30.

Key Laboratory of Advanced Display and System Applications of Ministry of Education, Shanghai University, 149 Yanchang Road, Shanghai, 200072, China.

Although excellent performance such as high efficiency and stability have been achieved in quantum dot (QD)-based light-emitting diodes (QLEDs) possessing an organic/inorganic hybrid device structure, the highly expected all-inorganic QLEDs remain at the bottleneck stage in recent years, resulting from the luminance quenching of QDs caused by inorganic hole transport layer (HTL) and unbalanced charge injection due to large energy barrier for injecting holes from HTL to QDs. Here, it is reported that the solution-processed inorganic environmentally friendly chloride (Cl)-passivated tungsten phosphate (Cl@TPA) films serve as HTL. The incorporation of Cl in TPA effectively passivates the oxygen vacancies, which not only avoids the luminescence quenching of QDs by reducing carrier concentration but also facilitates the hole injection from HTL to QDs with a favorable electronic band alignment, thus achieving the record external quantum efficiency of ≈9.27%, among all previous reports about all-inorganic QLEDs. Most importantly, the resulting all-inorganic QLEDs with Cl@TPA exhibit a substantial improvement in the operational lifetime (T  > 10 h under an initial luminance of 100 cd m ), which is almost 30-fold higher than the devices with TPA HTL. This work furnishes a promising strategy for highly efficient and stable QLEDs based on inorganic device structure.
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http://dx.doi.org/10.1002/smll.202100030DOI Listing
March 2021

Ghrelin Inhibits Intestinal Epithelial Cell Apoptosis Through the Unfolded Protein Response Pathway in Ulcerative Colitis.

Front Pharmacol 2021 10;12:661853. Epub 2021 Mar 10.

Department of Gastroenterology, Jinshan Hospital, Fudan University, Shanghai, China.

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) that occurs in the lining of the rectum and colon. Apoptosis of the intestinal epithelial cells (IECs) is common in active UC patients. Ghrelin is reported to be downregulated in apoptosis of IECs induced by tumor necrosis factor-α (TNF-α). Therefore, we hypothesized that ghrelin might play an antiapoptotic role in UC progression, which was investigated using and studies. The TNF-α-treated Caco-2 cell model and mouse colitis model induced by dextran sulfate sodium (DSS) or 2,4,6-trinitrobenzenesulfonic acid (TNBS) were established and employed. We found that ghrelin could inhibit the apoptosis of Caco-2 cells induced by TNF-α, which could be disturbed by [D-lys3]-GHRP-6, the antagonist of ghrelin receptor GHS-R1a. Similarly, in the DSS- and TNBS-induced mouse colitis models, ghrelin could also protect intestinal tissues from apoptosis in DSS- and TNBS-induced colitis depending on GHS-R1a. Furthermore, ghrelin modulated the unfolded protein response (UPR) pathway and regulated the expressions of caspase-3, BAX, and Bcl-2, which contributed to the inhibition of cell apoptosis. In conclusion, ghrelin protects IECs from apoptosis during the pathogenesis of colitis by regulating the UPR pathway.
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http://dx.doi.org/10.3389/fphar.2021.661853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988211PMC
March 2021

Factors leading to the risk of stroke mortality: a cross-sectional study with lung cancer patient-based large sample.

Eur J Cancer Prev 2021 Mar 23. Epub 2021 Mar 23.

Department of Respiratory Medicine Neurology, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, Zhejiang Shenzhen Mental Health Center, Shenzhen Key Laboratory of Mental Health, Shenzhen Kangning Hospital, Shenzhen, China.

To identify the risk factors for stroke mortality among lung cancer patients on the basis of the Surveillance, Epidemiology, and End Results (SEER) database. The clinical data of lung cancer patients diagnosed between 2004 and 2016 were collected in the SEER database. The stroke mortality of lung cancer patients was compared with the general population using standardized mortality ratios (SMRs). COX proportional hazard model was applied to analyze the risk factors for stroke mortality among lung cancer patients. Among 82 454 patients, 4821 (5.85%) died of stroke. The stroke mortality rate in lung cancer patients significantly increased compared with the general population [SMR: 1.73, 95% confidential interval (95% CI), 1.69-1.78]. Differences were pronounced between the patients with stroke death and those without regarding all the basic characteristics (P < 0.001). Multivariate COX analysis showed that the risk factors for stroke mortality among lung cancer patients included increasing age, males, the black, grade II-III, distant metastasis and higher American Joint Committee on Cancer (AJCC) TNM stage, whereas adenocarcinoma was found to be a protective factor compared with squamous cell carcinoma. Increasing age, males, the black, grade II-III, distant metastasis and higher TNM stage are associated with an increased risk of stroke mortality among lung cancer patients, but adenocarcinoma with a lowered risk.
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http://dx.doi.org/10.1097/CEJ.0000000000000675DOI Listing
March 2021

Deficiency of telomere-associated repressor activator protein 1 precipitates cardiac aging in mice p53/PPARα signaling.

Theranostics 2021 4;11(10):4710-4727. Epub 2021 Mar 4.

State Key Laboratory of Pharmaceutical Biotechnology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Telomere shortening and dysfunction may cause metabolic disorders, tissue damage and age-dependent pathologies. However, little is known about the association of telomere-associated protein Rap1 with mitochondrial energy metabolism and cardiac aging. Echocardiography was performed to detect cardiac structure and function in Rap1 and Rap1 mice at different ages (3 months, 12 months and 20 months). Telomere length, DNA damage, cardiac senescence and cardiomyocyte size were analyzed using the real-time PCR, Western blotting, senescence associated β-galactosidase assay and wheat germ agglutinin staining, respectively. Western blotting was also used to determine the level of cardiac fatty acid metabolism related key enzymes in mouse and human myocardium. Chromatin immunoprecipitation assay was used to verify the direct link between p53 and PPARα. The p53 inhibitor, Pifithrin-α and PPARα activator WY14643 were utilized to identify the effects of Rap1/p53/PPARα signaling pathway. Telomere was shortened concomitant with extensive DNA damage in aged Rap1 mouse hearts, evidenced by reduced T/S ratios and increased nuclear γH2AX. Meanwhile, the aging-associated phenotypes were pronounced as reflected by altered mitochondrial ultrastructure, enhanced senescence, cardiac hypertrophy and dysfunction. Mechanistically, acetylated p53 and nuclear p53 was enhanced in the Rap1 mouse hearts, concomitant with reduced PPARα. Importantly, p53 directly binds to the promoter of PPARα in mouse hearts and suppresses the transcription of PPARα. In addition, aged Rap1 mice exhibited reduced cardiac fatty acid metabolism. Pifithrin-α alleviated cardiac aging and enhanced fatty acid metabolism in the aged Rap1 mice. Activating PPARα with WY14643 in primarily cultured Rap1 cardiomyocytes restored maximal oxygen consumption rates. Reduced Rap1 expression and impaired p53/PPARα signaling also presented in aged human myocardium. In summary, Rap1 may link telomere biology to fatty acid metabolism and aging-related cardiac pathologies modulating the p53/PPARα signaling pathway, which could represent a therapeutic target in preventing/attenuating cardiac aging.
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http://dx.doi.org/10.7150/thno.51739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978321PMC
March 2021

Sufentanil Protects the Liver from Ischemia/Reperfusion-Induced Inflammation and Apoptosis by Inhibiting ATF4-Induced TP53BP2 Expression.

Inflammation 2021 Mar 10. Epub 2021 Mar 10.

Department of Anesthesiology, Anhui Provincial Hospital, Cheeloo College of Medicine, Shangdong University, 17 Lujiang Road, Hefei, 230000, Anhui, China.

Liver ischemia-reperfusion (I/R) injury is a pathological process that often occurs during liver and trauma surgery. This study aimed to investigate the protective effect and potential mechanisms of sufentanil on hepatic I/R injury. I/R rat model and hypoxic/reoxygenation (H/R)-induced buffalo rat liver (BRL)-3A cell model were established. Following pretreatment with sufentanil, the enzymatic activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in rat serum and the changes of hepatic histopathology were evaluated to track the extent of liver injury. The levels of inflammatory factors were determined with ELISA kits and RT-qPCR. The infiltration of macrophages was assessed after detecting monocyte chemoattractant protein 1 (MCP-1) and F4/80 expression. Additionally, apoptosis was measured by means of TUNEL staining, and gene expression related to apoptosis was examined using RT-qPCR and western blotting. Then, TP53BP2 was overexpressed in BRL-3A cells exposed to H/R condition to evaluate whether sufentanil defended the liver against injury by regulating TP53BP2 expression. Moreover, the potential binding site of ATF4 on the TP53BP2 promoter was analyzed using JASPAR databases and verified by chromosomal immunoprecipitation (ChIP) assay. Furthermore, TP53BP2 expression and endoplasmic reticulum stress (ERS)-related protein levels were determined after ATF4 was overexpressed in sufentanil-treated BRL-3A cells. Results revealed that sufentanil significantly improved hepatic I/R injury, decreased the levels of inflammatory factors, and alleviated hepatocyte apoptosis. Notably, upregulated TP53BP2 expression was observed in hepatic tissues, and TP53BP2 overexpression markedly reversed the protective effects of sufentanil on the inflammation and apoptosis in H/R-stimulated BRL-3A cells. Additionally, ATF4 was confirmed to combine with the TP53BP2 promoter. ATF4 upregulation attenuated the inhibitory effects of sufentanil on the expression of TP53BP2 and ERS-associated proteins. These findings demonstrated that sufentanil protects the liver from inflammation and apoptosis injury induced by I/R by inhibiting ATF4 expression and further suppressing TP53BP2 expression, suggesting a promising therapeutic candidate for the treatment of liver I/R injury.
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http://dx.doi.org/10.1007/s10753-020-01410-xDOI Listing
March 2021

Biomimetic oxygen delivery nanoparticles for enhancing photodynamic therapy in triple-negative breast cancer.

J Nanobiotechnology 2021 Mar 20;19(1):81. Epub 2021 Mar 20.

Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Triple-negative breast cancer (TNBC) is a kind of aggressive breast cancer with a high rate of metastasis, poor overall survival time, and a low response to targeted therapies. To improve the therapeutic efficacy and overcome the drug resistance of TNBC treatments, here we developed the cancer cell membrane-coated oxygen delivery nanoprobe, CCm-HSA-ICG-PFTBA, which can improve the hypoxia at tumor sites and enhance the therapeutic efficacy of the photodynamic therapy (PDT), resulting in relieving the tumor growth in TNBC xenografts.

Results: The size of the CCm-HSA-ICG-PFTBA was 131.3 ± 1.08 nm. The in vitro O and ROS concentrations of the CCm-HSA-ICG-PFTBA group were both significantly higher than those of the other groups (P < 0.001). In vivo fluorescence imaging revealed that the best time window was at 24 h post-injection of the CCm-HSA-ICG-PFTBA. Both in vivo F-FMISO PET imaging and ex vivo immunofluorescence staining results exhibited that the tumor hypoxia was significantly improved at 24 h post-injection of the CCm-HSA-ICG-PFTBA. For in vivo PDT treatment, the tumor volume and weight of the CCm-HSA-ICG-PFTBA with NIR group were both the smallest among all the groups and significantly decreased compared to the untreated group (P < 0.01). No obvious biotoxicity was observed by the injection of CCm-HSA-ICG-PFTBA till 14 days.

Conclusions: By using the high oxygen solubility of perfluorocarbon (PFC) and the homologous targeting ability of cancer cell membranes, CCm-HSA-ICG-PFTBA can target tumor tissues, mitigate the hypoxia of the tumor microenvironment, and enhance the PDT efficacy in TNBC xenografts. Furthermore, the HSA, ICG, and PFC are all FDA-approved materials, which render the nanoparticles highly biocompatible and enhance the potential for clinical translation in the treatment of TNBC patients.
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http://dx.doi.org/10.1186/s12951-021-00827-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981819PMC
March 2021

MSCs-engineered biomimetic PMAA nanomedicines for multiple bioimaging-guided and photothermal-enhanced radiotherapy of NSCLC.

J Nanobiotechnology 2021 Mar 20;19(1):80. Epub 2021 Mar 20.

Department of Radiation Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.

Background: The recently developed biomimetic strategy is one of the mostly effective strategies for improving the theranostic efficacy of diverse nanomedicines, because nanoparticles coated with cell membranes can disguise as "self", evade the surveillance of the immune system, and accumulate to the tumor sites actively.

Results: Herein, we utilized mesenchymal stem cell memabranes (MSCs) to coat polymethacrylic acid (PMAA) nanoparticles loaded with Fe(III) and cypate-an derivative of indocyanine green to fabricate Cyp-PMAA-Fe@MSCs, which featured high stability, desirable tumor-accumulation and intriguing photothermal conversion efficiency both in vitro and in vivo for the treatment of lung cancer. After intravenous administration of Cyp-PMAA-Fe@MSCs and Cyp-PMAA-Fe@RBCs (RBCs, red blood cell membranes) separately into tumor-bearing mice, the fluorescence signal in the MSCs group was 21% stronger than that in the RBCs group at the tumor sites in an in vivo fluorescence imaging system. Correspondingly, the T-weighted magnetic resonance imaging (MRI) signal at the tumor site decreased 30% after intravenous injection of Cyp-PMAA-Fe@MSCs. Importantly, the constructed Cyp-PMAA-Fe@MSCs exhibited strong photothermal hyperthermia effect both in vitro and in vivo when exposed to 808 nm laser irradiation, thus it could be used for photothermal therapy. Furthermore, tumors on mice treated with phototermal therapy and radiotherapy shrank 32% more than those treated with only radiotherapy.

Conclusions: These results proved that Cyp-PMAA-Fe@MSCs could realize fluorescence/MRI bimodal imaging, while be used in phototermal-therapy-enhanced radiotherapy, providing desirable nanoplatforms for tumor diagnosis and precise treatment of non-small cell lung cancer.
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http://dx.doi.org/10.1186/s12951-021-00823-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981797PMC
March 2021

Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model.

Biomed Pharmacother 2021 Mar 16;138:111466. Epub 2021 Mar 16.

School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China; Anhui Provincial Key Laboratory of Population Health and Aristogenics, Hefei 230032, Anhui, China. Electronic address:

Rhizoma drynariae total flavonoids (RDTF) are extracted from Drynaria fortunei J. Sm (D. fortunei), which was a Chinese herb commonly used to treat fractures and bruises. Modern pharmacological studies indicate flavonoids have anti-inflammatory effect in clinical practice. However, its active ingredients and the mechanisms of action are far from clear. The present study aims to determine whether RDTF can protect against intervertebral disc degeneration in a rat cervical intervertebral disc model and investigate the associated molecular mechanisms. Sprague Dawley (SD) rats were randomized into five groups: control group (CG, n = 8), intervertebral disc degeneration group (NG, n = 8), low-dose RDTF-treated group (LG, n = 8), medium-dose RDTF-treated group (MG, n = 8), and high-dose RDTF-treated group (HG, n = 8). Hematoxylin and eosin (HE) staining, immunohistochemistry (IHC), immunofluorescence, ELISA, Western blot and quantitative real time PCR (qRT-PCR) assays were used to investigate inflammatory, catabolic factors and the latent regulatory mechanism of the effects of RDTF on intervertebral disc cells. HE staining showed disc degeneration in all groups except CG, and the function was restored after RDTF treatment. IHC, Western blot, qRT-PCR, immunofluorescence and ELISA results showed that RDTF prevented intervertebral disc degeneration by suppressing mitogen-activated protein kinase (MAPK) pathway, which reduced expression of intracellular matrix metalloproteinases (MMPs), such MMP3, MMP13, and inflammatory factors including interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α). Notably RDTF inhibited extracellular matrix (ECM) degeneration by increasing expression of aggrecan and collagen type II and preventing the upregulation of collagen type I and III. It suggests that RDTF has a potential therapeutic effect on cervical spondylosis.
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http://dx.doi.org/10.1016/j.biopha.2021.111466DOI Listing
March 2021

Corrigendum to "Fibrin degradation by rtPA enhances the delivery of nanotherapeutics to A549 tumors in nude mice" [Biomaterials (2016) 63-71].

Biomaterials 2021 Apr 15;271:120744. Epub 2021 Mar 15.

School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai, 201203, PR China.

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http://dx.doi.org/10.1016/j.biomaterials.2021.120744DOI Listing
April 2021

Rapid 3D reconstruction method based on the polarization-enhanced fringe pattern of an HDR object.

Opt Express 2021 Jan;29(2):2162-2171

Measurement of high dynamic range objects is an obstacle in structured light 3D measurement. They entail both over-exposed and low-exposed pixels in a single exposure. This paper proposed a polarization-enhanced fringe pattern (PEFP) method that a high dynamic range image can be obtained within a single exposure time. The degree of linear polarization (DOLP) is calculated using the polarization properties of reflected light and a linear polarizer in fixed azimuth in this method. The DOLP is efficiently estimated by the projected polarization-state-encode (PSE) pattern, and it does not need to change the state of the polarizer. The DOLP depends on light intensity rather than the reflectivity of the object surfaces indicated in experimental results. The contrast of fringe patterns was enhanced, and the quality of fringe patterns was improved by the proposed method. More sufficient 3D point clouds and high-quality shape can be recovered using this method.
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http://dx.doi.org/10.1364/OE.416179DOI Listing
January 2021

Cascade Drug-Release Strategy for Enhanced Anticancer Therapy.

Matter 2021 Jan 6;4(1):26-53. Epub 2021 Jan 6.

Yong Loo Lin School of Medicine and Faculty of Engineering, National University of Singapore, Singapore, 117597, Singapore.

Chemotherapy serves as one of the most effective approaches in numerous tumor treatments but also suffers from the limitations of low bioavailability and adverse side effects due to premature drug leakage. Therefore, it is crucial to realize accurate on-demand drug release for promoting the application of chemotherapeutic agents. To achieve this, stimuli-responsive nanomedicines that can be activated by delicately designed cascade reactions have been developed in recent years. In general, the nanomedicines are triggered by an internal or external stimulus, generating an intermediate stimulus at tumor site, which can intensify the differences between tumor and normal tissues; the drug release process is then further activated by the intermediate stimulus. In this review, the latest progress made in cascade reactions-driven drug-release modes, based on the intermediate stimuli of heat, hypoxia, and reactive oxygen species, is systematically summarized. The perspectives and challenges of cascade strategy for drug delivery are also discussed.
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http://dx.doi.org/10.1016/j.matt.2020.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945719PMC
January 2021

Endoscopic full-thickness resection using an over-the-scope device: A prospective study.

World J Gastroenterol 2021 Feb;27(8):725-736

Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: Endoscopic submucosal dissection to treat mucosal and submucosal lesions sometimes results in low rates of microscopically margin-negative (R0) resection. Endoscopic full-thickness resection (EFTR) has a high R0 resection rate and allows for the definitive diagnosis and treatment of selected mucosal and submucosal lesions that are not suitable for conventional resection techniques.

Aim: To evaluate the efficacy and safety of EFTR using an over-the-scope clip (OTSC).

Methods: This prospective, single-center, non-randomized clinical trial was conducted at the endoscopy center of Shengjing Hospital of China Medical University. The study included patients aged 18-70 years who had gastric or colorectal submucosal tumors (SMTs) (≤ 20 mm in diameter) originating from the muscularis propria based on endoscopic ultrasound (EUS) and patients who had early-stage gastric or colorectal cancer (≤ 20 mm in diameter) based on EUS and computed tomography. All lesions were treated by EFTR combined with an OTSC for wound closure between November 2014 and October 2016. We analyzed patient demographics, lesion features, histopathological diagnoses, R0 resection (negative margins) status, adverse events, and follow-up results.

Results: A total of 68 patients (17 men and 51 women) with an average age of 52.0 ± 10.5 years (32-71 years) were enrolled in this study, which included 66 gastric or colorectal SMTs and 2 early-stage colorectal cancers. The mean tumor diameter was 12.6 ± 4.3 mm. The EFTR procedure was successful in all cases. The mean EFTR procedure time was 39.6 ± 38.0 min. The mean OTSC defect closure time was 5.0 ± 3.8 min, and the success rate of closure for defects was 100%. Histologically complete resection (R0) was achieved in 67 (98.5%) patients. Procedure-related adverse events were observed in 11 (16.2%) patients. The average post-procedure length of follow-up was 48.2 ± 15.7 mo. There was no recurrence during follow-up.

Conclusion: EFTR combined with an OTSC is an effective and safe technique for the removal of select subepithelial and epithelial lesions that are not amenable to conventional endoscopic resection techniques.
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http://dx.doi.org/10.3748/wjg.v27.i8.725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934006PMC
February 2021

Profiles of differentially expressed long noncoding RNAs and messenger RNAs in the myocardium of septic mice.

Ann Transl Med 2021 Feb;9(3):199

Department of Critical Care Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

Background: Sepsis is the primary cause of mortality in the intensive care unit (ICU), mainly due to sepsis-induced dysfunction of essential organs such as the heart and lungs. This study investigated the myocardium's epigenetic characterization from septic mice to identify potential treatment targets for septic myocardial dysfunction.

Methods: Cecal ligation and puncture (CLP) was used to induce sepsis in male C57BL/6 mice. Hearts were collected 24 h after surgery to determine the expression profiles of long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) by microarray. To validate the reliability of microarray results, we randomly chose six differentially expressed lncRNAs for qRT-PCR. Functional mapping of differentially expressed mRNAs was annotated with gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses; lncRNA-mRNA co-expression network was constructed to reveal connections between lncRNAs and mRNAs.

Results: Microarray analysis indicated that 1,568 lncRNAs and 2,166 mRNAs were differentially expressed in the myocardium from septic mice, which was further confirmed by qRT-PCR. KEGG pathway analysis showed that numerous differentially expressed mRNAs were relevant to tumor necrosis factor (TNF) and phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signaling pathways. Moreover, according to the lncRNA-mRNA co-expression network constructed by the above six lncRNAs and their interacting mRNAs, the co-expression network profiles had 57 network nodes and 134 connections, including 76 positive interactions and 58 negative interactions.

Conclusions: In mouse hearts, sepsis resulted in differential expression of lncRNAs and mRNAs related to TNF and PI3K-Akt signaling pathways, suggesting that lncRNAs and their interacting mRNAs may participate in the pathogenesis of septic myocardial dysfunction by regulating TNF and PI3K-Akt signaling pathways.
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http://dx.doi.org/10.21037/atm-20-3830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940873PMC
February 2021

Surface-enhanced shifted excitation Raman difference spectroscopy for trace detection of fentanyl in beverages.

Appl Opt 2021 Mar;60(8):2354-2361

In recognition of the misuse risks of fentanyl, there is an urgent need to develop a useful and rapid analytical method to detect and monitor the opioid drug. The surface-enhanced shifted excitation Raman difference spectroscopy (SE-SERDS) method has been demonstrated to suppress background interference and enhance Raman signals. In this study, the SE-SERDS method was used for trace detection of fentanyl in beverages. To prepare the simulated illegal drug-beverages, fentanyls were dissolved into distilled water or Mizone as a series of test samples. Based on our previous work, the surface-enhanced Raman spectroscopy detection was performed on the beverages containing fentanyl by the prepared AgNPs and the SE-SERDS spectra of test samples were collected by the dual-wavelength rapid excitation Raman difference spectroscopy system. In addition, the quantitative relationship between fentanyl concentrations and the Raman peaks was constructed by the Langmuir equation. The experimental results show that the limits of quantitation for fentanyl in distilled water and Mizone were 10 ng/mL and 200 ng/mL, respectively; the correlation coefficients for the nonlinear regression were as high as 0.9802 and 0.9794, respectively; and the relative standard deviation was less than 15%. Hence, the SE-SERDS method will be a promising method for the trace analyses of food safety and forensics.
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http://dx.doi.org/10.1364/AO.418579DOI Listing
March 2021

Association between hand grip strength and stroke in China: a prospective cohort study.

Aging (Albany NY) 2021 Mar 3;13(6):8204-8213. Epub 2021 Mar 3.

Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.

Objectives: The association between weak hand grip strength (HGS) and stroke recovery has been studied; however, few studies focused on the association of HGS with stroke prevalence and incidence.

Methods: A prospective cohort baseline study of a nationally representative sample in Chinese adults aged 45 years and older in 2011 was followed up in 2015. 8871 participants without stroke at baseline were followed. The associations of HGS and its changes with stroke prevalence and incidence were investigated using logistic regression models and Cox proportional hazards regression models.

Results: Association of HGS and stroke prevalence was significant. HGS weakness significantly increased risk of stroke incidence, with 89.3% higher risk when compared to normal HGS. During 35,263 person-years of follow-up, 112 stroke patients occurred. The four-year incidence rate ratio of stroke for participants with a HGS weakness was 2.15, compared to normal HGS participants. HGS changes in weakness/weakness from 2011 to 2015(D-HGS) and normal/weakness D-HGS had higher risks of stroke incidence when compared with those who had normal/normal D-HGS.

Conclusions: HGS weakness and decline of HGS were associated with stroke incidence for adults aged 45 years and older in China.
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http://dx.doi.org/10.18632/aging.202630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034956PMC
March 2021

THBS1/CD47 Modulates the Interaction of γ-Catenin With E-Cadherin and Participates in Epithelial-Mesenchymal Transformation in Lipid Nephrotoxicity.

Front Cell Dev Biol 2020 18;8:601521. Epub 2021 Feb 18.

State Key Laboratory of Natural Medicines, Department of Pharmacology, China Pharmaceutical University, Nanjing, China.

Hyperlipidemia, an important risk factor for cardiovascular and end-stage renal diseases, often aggravates renal injury and compromises kidney function. Here, histological analysis of human kidney samples revealed that high lipid levels induced the development of renal fibrosis. To elucidate the mechanism underlying lipid nephrotoxicity, we used two types of mouse models (Apoe and C57BL/6 mice fed a 45 and 60% high-fat diet, respectively). Histological analysis of kidney tissues revealed high-lipid-induced renal fibrosis and inflammation; this was confirmed by examining fibrotic and inflammatory marker expression using Western blotting and real-time polymerase chain reaction. Oxidized low-density lipoprotein (OX-LDL) significantly induced the fibrotic response in HK-2 tubular epithelial cells. RNA-sequencing and Gene Ontology analysis of differentially expressed mRNAs in OX-LDL-treated HK-2 tubular epithelial cells and real-time PCR validation in Apoe mice showed that the expression of thrombospondin-1 () in the high-fat group was significantly higher than that of the other top known genes, along with significant overexpression of its receptor CD47. knockdown cells verified its relation to OX-LDL-induced fibrosis and inflammation. Liquid chromatography tandem mass spectrometry and STRING functional protein association network analyses predicted that THBS1/CD47 modulated the interaction between γ-catenin and E-cadherin and was involved in epithelial-mesenchymal transition, which was supported by immunoprecipitation and immunohistochemistry. CD47 downregulation following transfection with small-hairpin RNA in OX-LDL-treated tubular epithelial cells and treatment with anti-CD47 antibody restored the expression of E-cadherin and attenuated renal injury, fibrosis, and inflammatory response in OX-LDL-treated cells and in type 2 diabetes mellitus. These findings indicate that CD47 may serve as a potential therapeutic target in long-term lipid-induced kidney injury.
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http://dx.doi.org/10.3389/fcell.2020.601521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930485PMC
February 2021

Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter.

Front Microbiol 2021 12;12:637347. Epub 2021 Feb 12.

Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, School of Life Science, Ningxia University, Yinchuan, China.

Reporter gene-based expression systems have been intensively used in plants for monitoring the activity of gene promoters. However, rRNA transcripts are unable to efficiently express a reporter gene due to a lack of a 5' cap. Because of this obstacle, plant rRNA gene promoters are less well characterized to this day. We developed a virus-based reporter system to characterize the rRNA (rRNA) gene promoter. The system utilizes the cap-independent translation strategy of viral genomic mRNA and uses the virus-expressed green fluorescent protein (GFP) as an indicator of the rRNA gene promoter activity in virus-infected plants. Based on the reporter system, some characteristics of the rRNA gene promoter were revealed. The results showed that the strength of the rRNA gene promoter was lower than that of the cauliflower mosaic virus (CaMV) 35S promoter, a well-characterized polymerase II promoter. The sequences between -77 and +42 are sufficient for the rRNA gene promoter-mediated transcription and the rRNA gene promoter may lack the functional upstream control element (UCE). Interestingly, rRNA gene promoter activity was increased when the 35S enhancer was introduced. An intron-excision mediated assay revealed that the rRNA gene promoter can be inefficiently used by RNA polymerase II in cells. This virus-based reporter system is easier to operate and more convenient when compared with the previously Pol I promoter assays. And it offers a promising solution to analyzing the functional architecture of plant rRNA gene promoter.
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http://dx.doi.org/10.3389/fmicb.2021.637347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928365PMC
February 2021

Continuous serratus anterior plane block improved early pulmonary function after lung cancer surgery.

Ann Thorac Surg 2021 Mar 2. Epub 2021 Mar 2.

Department of Anesthesiology, Pain Clinic, First Affiliated Hospital of USTC (Anhui Provincial Hospital), University of Science and Technology of China, Hefei 230001, Anhui, China. Electronic address:

Background: Serratus anterior plane block (SAPB) has been proven to be an efficient way to control postoperative pain. This study explored whether the use of continuous SAPB in combination with flurbiprofen could improve early pulmonary function in lung cancer patients undergoing video-assisted thoracoscopic surgery (VATS).

Methods: From July 2019 to April 2020, patients who scheduled for elective lung resection undergoing thoracoscopic surgery were randomly allocated to receive patient-controlled SAPB in combination with intravenous flurbiprofen or patient-controlled intravenous analgesia. Postoperative pulmonary function parameters, including forced expiratory volume in 1 second, and forced vital capacity were collected before and 24, 48, and 72 hours after surgery. Pain intensity was measured at rest and on coughing. Comfort score during breathing exercises, postoperative pulmonary complications and adverse events were recorded.

Results: A substantial reduction in lung function was exhibited in both groups after surgery (P < 0.001), but lung function parameters in continuous SAPB group were significantly higher (P < 0.001) throughout postoperative period up to 72 hours, regardless of the surgery type. Meanwhile, there were significant differences of pain intensity at rest and on coughing between both groups (P < 0.001). The incidence of pneumonia, pulmonary atelectasis, hypoxemia, vomiting and the comfort score in continuous SAPB group was significantly lower (P< 0.05).

Conclusions: Postoperative acute pain treatment with continuous SAPB in combination with flurbiprofen enhanced pulmonary function and reduced postoperative pulmonary complications in lung cancer patients undergoing VATS.
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http://dx.doi.org/10.1016/j.athoracsur.2021.02.032DOI Listing
March 2021

[Clinical effect of lever positioning manipulation for lumbar disc herniation and its influence on Cobb angle].

Zhongguo Gu Shang 2021 Jan;34(1):86-90

The Third Clinical Medical College of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang, China.

Objective: To observe the clinical effect of lever positioning manipulation for the treatment of lumbar disc herniation and its effect on Cobb angle.

Methods: From December 2017 to November 2018, 67 patients with lumbar disc herniation were included in the study. The patients were randomly divided into treatment group and control group by digital table method. There were 34 cases in the treatment group, including 20 males and 14 females, with an average age of (36.09±8.26) years old and a course of (13.79±15.50) months. Treatment group was treated with lever positioning manipulation. There were 33 cases in the control group, including 18 males and 15 females, with an average age of(36.48±7.81) years old and a course of (12.82±15.68) months. Control group was treated with lumbar slanting manipulation. Two groups were treated 3 times a week, once every other day, 6 times for a course of treatment, after 2 courses of treatment, the changes of Cobb angle before and after treatment were compared between two groups by imaging. The symptoms and signs were scored with reference to clinical evaluation standard;overall efficacy was evaluated with reference to "Diagnostic Efficacy Criteria of Traditional Chinese Medicine Syndrome" issued by the State Administration of Traditional Chinese Medicine for lumbar disc herniation.

Results: One patient in each group dropped out. The symptom and sign scores of treatment group and control group before treatment were 18.56± 4.81, 18.61±3.72, while after treatment were 9.41±5.19, 13.55±3.68;treatment group was significantly lower than control group after treatment (<0.05). The rate of overall efficacy of treatment group and control group were 97.06% and 75.76%, respectively, and treatment group was superiorto control group(<0.05). Post treatment Cobb angle of both groups of patients became smaller(<0.05). The Cobb angle of treatment group and control group were(17.95±4.45)°, (18.14±3.59)° before treatment, while after treatment were (18.14±3.59)°, (15.49±1.75)°, change of Cobb angle in treatment group was better than in controlgroup(<0.05).

Conclusion: Both the lever positioning manipulation and the lumbar slanting manipulation methods are effective for the treatment of lumbar disc herniation, but clinical effect of lever positioning method on lumbar disc herniation is more significant, and the effect on Cobb angle is more obvious. It is worthy of promotion.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.01.016DOI Listing
January 2021

Effects of morphine preconditioning on TRPV1 sensitization and ERK1/2 phosphorylation induced by TGFβ1 in neurocytes.

Neuroreport 2021 Mar;32(5):339-344

Department of Anesthesiology, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui, China.

Objective: Myocardium ischemia-reperfusion injury (IRI) is the major cause of cardiac dysfunction. While intrathecal morphine preconditioning (MPC) can alleviate IRI in animal model, the molecular processes underlying IRI and MPC remain elusive. This study aims to test whether pretreatment with morphine can ameliorate the increased activity of transient receptor potential vanilloid 1 (TRPV1) induced by transforming growth beta1 (TGFβ1) in cultured dorsal root ganglion neurons as a model of the effects of cardiac ischemia on nociceptive primary afferent neurons.

Methods: To simulate the effect of MPC on dorsal root ganglia (DRG) neurons during myocardial IRI in vivo, the cells were pretreated with morphine for 10 min, followed by wash-out for 30 min before TGFβ1 was added. Afterwards, DRG neurons and N2a cells in all groups were stimulated by capsaicin, and the inward current induced by capsaicin were detected by whole-cell recording on DRG neurons; the expression of TRPV1, phosphorylated (p) TRPV1, ERK1/2, and pERK1/2 were detected by western blot in N2a cells.

Results: In comparison with cells with normal culture, the inward current was enhanced of cells incubated with TGFβ1 (P < 0.05), and the relative expression of TRPV1, pTRPV1, and pERK1/2 was upregulated as well (P < 0.05); In comparison with cells incubated with TGFβ1, the inward current induced by capsaicin were decreased by pretreatment with morphine (P < 0.05), Moreover, the relative expression of TRPV1, pTRPV1, and pERK1/2 were also reduced by MPC (P < 0.05).

Conclusion: MPC inhibits TRPV1 sensitized by TGFβ1 in DRG cells, and the mechanism might be associated with the downregulation of pERK1/2 expression.
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http://dx.doi.org/10.1097/WNR.0000000000001603DOI Listing
March 2021

[Cloning and functional analysis of caffeic acid and rosmarinic acid glycosyltransferases from Arnebia euchroma].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(1):86-93

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.

Caffeic acid and its oligomers are the main water-soluble active constituents of the traditional Chinese medicine(TCM) Arnebiae Radix. These compounds possess multiple biological activities such as antimicrobial, antioxidant, cardiovascular protective, liver protective, anti-liver fibrosis, antiviral and anticancer activities. The phenylpropanoid pathway in plants is responsible for the biosynthesis of caffeic acid and its oligomers. Glycosylation can change phenylpropanoid solubility, stability and toxic potential, as well as influencing compartmentalization and biological activity. In view of the important role played by de-glycosylation in the regulation of phenylpropanoid homeostasis, the biosynthesis of caffeic acid and its oligomers are supposed to be under the control of relative UDP-glycosyltransferases(UGTs). Through the data mining of Arnebia euchroma transcriptome, we cloned 15 full-length putative UGT genes. After recombinant expression using the prokaryotic system, the crude enzyme solution of the putative UGTs was examined for the glycosylation activities towards caffeic acid and rosmarinic acid in vitro. AeUGT_01, AeUGT_02, AeUGT_03, AeUGT_04 and AeUGT_10 were able to glycosylate caffeic acid and/or rosmarinic acid resulting in different mono-and/or di-glycosylated products in the UPLC-MS analyses. The characterized UGTs were distantly related to each other and divided into different clades of the phylogenetic tree. Based on the observation that each characterized UGT exhibited substrate or catalytic similarity with the members in their own clade, we supposed the glycosylation abilities towards caffeic acid and/or rosmarinic acid were evolved independently in different clades. The identification of caffeic acid and rosmarinic acid UGTs from A. euchroma could lead to deeper understanding of the caffeic acid oligomers biosynthesis and its regulation. Furthermore, these UGTs might be used for regiospecific glycosylation of caffeic acid and rosmarinic acid to produce bioactive compounds for potential therapeutic applications.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200827.101DOI Listing
January 2021

Atrial fibrillation detection based on multi-feature extraction and convolutional neural network for processing ECG signals.

Comput Methods Programs Biomed 2021 Apr 17;202:106009. Epub 2021 Feb 17.

Department of Cardiovascular Surgery, Fuwai Central China Cardiovascular Hospital, Henan Cardiovascular Hospital and Zhengzhou University, Zhengzhou, China.

Background And Objective: The incidence of atrial fibrillation is increasing annually. We develop an automatic detection system, which is of great significance for the early detection and treatment of atrial fibrillation. This can lead to the reduction of the incidence of critical illnesses and mortality.

Methods: We propose an atrial fibrillation detection algorithm based on multi-feature extraction and convolutional neural network of atrial activity via electrocardiograph signals, and compare its detection based on cluster analysis, one-versus-one rule and support vector machine, using accuracy, specificity, sensitivity and true positive rate as evaluation criteria.

Results: The atrial fibrillation detection algorithm proposed in this paper has an accuracy rate of 98.92%, a specificity of 97.04%, a sensitivity of 97.19%, and a true positive rate of 96.47%. The average accuracy of the algorithms we compared is 80.26%, and the accuracy of our algorithm is 23.25% higher than this average pertaining to the other algorithms.

Conclusion: We implemented an atrial fibrillation detection algorithm that meets the requirements of high accuracy, robustness and generalization ability. It has important clinical and social significance for early detection of atrial fibrillation, improvement of patient treatment plans and improvement of medical diagnosis.
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http://dx.doi.org/10.1016/j.cmpb.2021.106009DOI Listing
April 2021

Elevated Creatinine Clearance in Lupus Nephritis patients with Normal Creatinine.

Int J Med Sci 2021 29;18(6):1449-1455. Epub 2021 Jan 29.

Department of Rheumatology, Qilu Hospital of Shandong University, Ji'nan 250012, Shandong, China.

The present study aimed to observe the differences in creatinine clearance (Ccr) in systemic lupus erythematosus (SLE) patients with normal serum creatinine at different levels of urinary protein. The present cross-sectional study included 177 SLE patients with normal serum creatinine from Qilu Hospital of Shandong University between January 2010 and April 2020. The following data were collected: blood urea nitrogen (BUN), serum creatinine (Cr), serum total protein, serum albumin, immunoglobulin (Ig) G, IgA, IgM, complement 3, complement 4, anti-ds-DNA antibody, routine urine test, urine protein/creatinine ratio (UPCR) (g/g), and the SLE disease activity index. The estimated Ccr was calculated according to the Cockcroft formula. 123 patients were with positive urinary protein (Lupus Nephritis, LN group) and 54 patients were with negative urinary protein (Non-LN group). Compared with the Non-LN group, the LN group had higher BUN (5.76±3.22 . 4.78±1.58, P=0.007) and Cr (62.36±19.53 . 54.83±11.09, P=0.001). There was a strong correlation between the UPCR and the semi-quantitative determination of urine protein in LN patients (r=0.9583, P=0.0417). The serum creatinine levels were significantly higher in patients with urine protein 3+ (72.97±25.16) or massive proteinuria (62.32±19.66) than the other groups. Patients with urinary protein ± exhibited a significantly elevated Ccr when compared to patients with urinary protein 3+ (130.6±44.15 110.5±33.50, P=0.02), and patients with UPCR<0.15 g/g had higher Ccr than other groups and showed significantly increased Ccr compared with patients with UPCR≥0.15 g/g (132.44±21.02 115.14±35.89, P=0.007). Early renal function impairment may be present in LN patients. The kidneys of LN patients with urinary protein ± or UPCR<0.15 g/g are in a state of hyperfunction.
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http://dx.doi.org/10.7150/ijms.51117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893553PMC
January 2021

Effects of Self-Assisted Shoulder Elevation of the Affected Side Combined with Balance Training on Associated Reactions of Upper Limb and Walking Function in Chronic Stroke Patients: A Randomized Controlled Trial.

Med Sci Monit 2021 Feb 24;27:e928549. Epub 2021 Feb 24.

Department of Rehabilitation Medicine, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, Jiangsu, China (mainland).

BACKGROUND Associated reactions of the upper limb are frequently seen in stroke patients, especially during dynamic activities, such as walking. The aim of this study was to assess the effect of a method to inhibit the affected upper limb flexors combined with balance training on associated reactions of the affected upper limb and walking function in chronic stroke patients. MATERIAL AND METHODS 60 patients were randomly allocated into 3 groups (n=20 per group): control group (no upper limb intervention), back group (the unaffected hand assists the affected upper limb in the low back and keep it in an extended position) and shoulder elevation group using the inhibition method (the unaffected hand assists the affected shoulder to elevate above 90°). Before and after the four-week balance training, the surface electromyography was used to evaluate the rate of contraction of affected elbow flexors. Fugl-Meyer Assessment of Upper Extremity (FMA-UE), 10 Meter Walking Test (10MWT) and Barthel Index (BI) were used to measure functional status. RESULTS The shoulder elevation group had significant improvement in the percentage changes in the rate of contraction of the affected elbow flexors, 10WMT and FMA-UE (p<0.05) compared with back group and control group. We found no significant difference of 10WMT and FMA-UE between back group and control group. CONCLUSIONS The combination of the new inhibition method and the standing balance training could reduce the abnormal activity of affected elbow flexors during walking, increase walking speed, and improve the affected upper limb motor function.
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http://dx.doi.org/10.12659/MSM.928549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919230PMC
February 2021