Publications by authors named "Sheng Peng"

116 Publications

A Robust Oxygen Microbubble Radiosensitizer for Iodine-125 Brachytherapy.

Adv Sci (Weinh) 2021 04 10;8(7):2002567. Epub 2021 Feb 10.

Department of Imaging and Interventional Radiology Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Guangzhou 510060 P. R. China.

Iodine-125 (I) brachytherapy, a promising form of radiotherapy, is increasingly applied in the clinical treatment of a wide range of solid tumors. However, the extremely hypoxic microenvironment in solid tumors can cause hypoxia-induced radioresistance to I brachytherapy, resulting in therapeutic inefficacy. In this study, the aim is to sensitize hypoxic areas in solid tumors using ultrasound-activated oxygen microbubbles for I brachytherapy. A modified emulsion freeze-drying method is developed to prepare microbubbles that can be lyophilized for storage and easily reconstituted in situ before administration. The filling gas of the microbubbles is modified by the addition of sulfur hexafluoride to oxygen such that the obtained O/SF microbubbles (OS MBs) achieve a much longer half-life (>3×) than that of oxygen microbubbles. The OS MBs are tested in nasopharyngeal carcinoma (CNE2) tumor-bearing mice and oxygen delivery by the OS MBs induced by ultrasound irradiation relieve hypoxia instantly. The post-treatment results of brachytherapy combined with the ultrasound-triggered OS MBs show a greatly improved therapeutic efficacy compared with brachytherapy alone, illustrating ultrasound-mediated oxygen delivery with the developed OS MBs as a promising strategy to improve the therapeutic outcome of I brachytherapy in hypoxic tumors.
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http://dx.doi.org/10.1002/advs.202002567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025033PMC
April 2021

Neobavaisoflavone Induces Bilirubin Metabolizing Enzyme UGT1A1 via PPARα and PPARγ.

Front Pharmacol 2020 8;11:628314. Epub 2021 Feb 8.

Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

UDP-glucuronosyltransferase 1A1 (UGT1A1) is an essential enzyme in mammals that is responsible for detoxification and metabolic clearance of the endogenous toxin bilirubin and a variety of xenobiotics, including some crucial therapeutic drugs. Discovery of potent and safe UGT1A1 inducers will provide an alternative therapy for ameliorating hyperbilirubinaemia and drug-induced hepatoxicity. This study aims to find efficacious UGT1A1 inducer(s) from natural flavonoids, and to reveal the mechanism involved in up-regulating of this key conjugative enzyme by the flavonoid(s) with strong UGT1A1 induction activity. Among all the tested flavonoids, neobavaisoflavone (NBIF) displayed the most potent UGT1A1 induction activity, while its inductive effects were confirmed by both western blot and glucuronidation activity assays. A panel of nuclear receptor reporter assays demonstrated that NBIF activated PPARα and PPARγ in a dose-dependent manner. Meanwhile, we also found that NBIF could up-regulate the expression of PPARα and PPARγ in hepatic cells, suggesting that the induction of UGT1A1 by NBIF was mainly mediated by PPARs. In silico simulations showed that NBIF could stably bind on pocket II of PPARα and PPARγ. Collectively, our results demonstrated that NBIF is a natural inducer of UGT1A1, while this agent induced UGT1A1 mainly via activating and up-regulating PPARα and PPARγ. These findings suggested that NBIF can be used as a promising lead compound for the development of more efficacious UGT1A1 inducers to treat hyperbilirubinaemia and UGT1A1-associated drug toxicities.
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http://dx.doi.org/10.3389/fphar.2020.628314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897654PMC
February 2021

Hypothermic oxygenated perfusion inhibits HECTD3-mediated TRAF3 polyubiquitination to alleviate DCD liver ischemia-reperfusion injury.

Cell Death Dis 2021 Feb 24;12(2):211. Epub 2021 Feb 24.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Engineering Research Center of Natural Polymer-based Medical Materials in Hubei Province, Wuhan, China.

Ischemia-reperfusion injury (IRI) is an inevitable and serious clinical problem in donations after heart death (DCD) liver transplantation. Excessive sterile inflammation plays a fateful role in liver IRI. Hypothermic oxygenated perfusion (HOPE), as an emerging organ preservation technology, has a better preservation effect than cold storage (CS) for reducing liver IRI, in which regulating inflammation is one of the main mechanisms. HECTD3, a new E3 ubiquitin ligase, and TRAF3 have an essential role in inflammation. However, little is known about HECTD3 and TRAF3 in HOPE-regulated liver IRI. Here, we aimed to investigate the effects of HOPE on liver IRI in a DCD rat model and explore the roles of HECTD3 and TRAF3 in its pathogenesis. We found that HOPE significantly improved liver damage, including hepatocyte and liver sinusoidal endothelial cell injury, and reduced DCD liver inflammation. Mechanistically, both the DOC and HECT domains of HECTD3 directly interacted with TRAF3, and the catalytic Cys (C832) in the HECT domain promoted the K63-linked polyubiquitination of TRAF3 at Lys138. Further, the ubiquitinated TRAF3 at Lys138 increased oxidative stress and activated the NF-κB inflammation pathway to induce liver IRI in BRL-3A cells under hypoxia/reoxygenation conditions. Finally, we confirmed that the expression of HECTD3 and TRAF3 was obviously increased in human DCD liver transplantation specimens. Overall, these findings demonstrated that HOPE can protect against DCD liver transplantation-induced-liver IRI by reducing inflammation via HECTD3-mediated TRAF3 K63-linked polyubiquitination. Therefore, HOPE regulating the HECTD3/TRAF3 pathway is a novel target for improving IRI in DCD liver transplantation.
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http://dx.doi.org/10.1038/s41419-021-03493-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904838PMC
February 2021

A network-based method for mechanistic investigation and neuroprotective effect on treatment of tanshinone Ⅰ against ischemic stroke in mouse.

J Ethnopharmacol 2021 May 20;272:113923. Epub 2021 Feb 20.

Medical College, Institute of Translational Medicine, Department of Neurology, Affiliated Hospital of Yangzhou University, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, PR China. Electronic address:

Ethnopharmacological Relevance: Tanshinone-Ⅰ (TSNⅠ), a member of the mainly active components of Salvia miltiorrhiza Bunge (Dan Shen), which is widely used for the treatment for modern clinical diseases including cardiovascular and cerebrovascular diseases, has been reported to show the properties of anti-oxidation, anti-inflammation, neuroprotection and other pharmacological actions. However, whether TSNⅠ can improve neuron survival and neurological function against transient focal cerebral ischemia (tMCAO) in mice is still a blank field.

Aim Of The Study: This study aims to investigate the neuroprotective effects of TSNⅠ on ischemic stroke (IS) induced by tMCAO in mice and explore the potential mechanism of TSNⅠ against IS by combining network pharmacology approach and experimental verification.

Materials And Methods: In this study, the pivotal candidate targets of TSNⅠ against IS were screened by network pharmacology firstly. Enrichment analysis and molecular docking of those targets were performed to identify the possible mechanism of TSNⅠ against IS. Afterwards, experiments were carried out to further verify the mechanism of TSNⅠ against IS. The infarct volume and neurological deficit were evaluated by 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining and Longa respectively. Immunohistochemistry was used to observe neuronal death in the hippocampus and cortical regions by detecting the change of NeuN. The predicting pathways of signaling-related proteins were assessed by Western blot in vitro and in vivo experiments.

Results: In vivo, TSNⅠ was found to dose-dependently decrease mice's cerebral infarct volume induced by tMCAO. In vitro, pretreatment with TSNⅠ could increase cell viability of HT-22 cell following oxygen-glucose deprivation (OGD/R). Moreover, the results showed that 125 candidate targets were identified, Protein kinase B (AKT) signaling pathway was significantly enriched by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and mitogen-activated protein kinases 1 (MAPK1) and AKT1 could be bound to TSNⅠ more firmly by molecular docking analysis, which implies that TSNⅠ may play a role in neuroprotection through activating AKT and MAPK signaling pathways. Meanwhile, TSNⅠ was confirmed to significantly protect neurons from injury induced by IS through activating AKT and MAPK signaling pathways.

Conclusion: In conclusion, our study clarifies that the mechanism of TSNⅠ against IS might be related to AKT and MAPK signaling pathways, which may provide the basic evidence for further development and utilization of TSNⅠ.
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http://dx.doi.org/10.1016/j.jep.2021.113923DOI Listing
May 2021

INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance.

Plant Cell Environ 2021 Feb 12. Epub 2021 Feb 12.

Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Research and Development of Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.

Transposable elements exist widely throughout plant genomes and play important roles in plant evolution. Auxin is an important regulator that is traditionally associated with root development and drought stress adaptation. The DEEPER ROOTING 1 (DRO1) gene is a key component of rice drought avoidance. Here, we identified a transposon that acts as an autonomous auxin-responsive promoter and its presence at specific genome positions conveys physiological adaptations related to drought avoidance. Rice varieties with a high and auxin-mediated transcription of DRO1 in the root tip show deeper and longer root phenotypes and are thus better adapted to drought. The INDITTO2 transposon contains an auxin response element and displays auxin-responsive promoter activity; it is thus able to convey auxin regulation of transcription to genes in its proximity. In the rice Acuce, which displays DRO1-mediated drought adaptation, the INDITTO2 transposon was found to be inserted at the promoter region of the DRO1 locus. Transgenesis-based insertion of the INDITTO2 transposon into the DRO1 promoter of the non-adapted rice variety Nipponbare was sufficient to promote its drought avoidance. Our data identify an example of how transposons can act as promoters and convey hormonal regulation to nearby loci, improving plant fitness in response to different abiotic stresses.
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http://dx.doi.org/10.1111/pce.14029DOI Listing
February 2021

Confined Generation of Homogeneously Dispersed Au and SnO Nanoparticles in Layered Silicate as Synergistic Catalysts.

Langmuir 2021 Feb 9;37(7):2341-2348. Epub 2021 Feb 9.

Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry, Beihang University, Beijing 100191, PR China.

With the aid of the confined conversion of layered silicate RUB-15, homogeneously dispersed Au and SnO nanoparticles (NPs) were generated in the confined layer space of RUB-15. The Au-SnO/SiO composite was obtained with the structure that ultrafine Au and SnO NPs were supported on SiO lamellas. Benefited by the Sn(II)-assisted in situ reduction strategy, Au NPs were highly uniformed and evenly distributed in/on the RUB-15. This Au-SnO/SiO composite was employed as a catalyst to the reduction of 4-nitrophenol showing excellent catalytic activity. The catalytic rate constant at room temperature was calculated to be 6.64 min, which was dramatically higher than that of Au/SiO composite produced by reduction with hydrazine hydrate on the same support of layered silicate RUB-15. The interaction between Au and SnO NPs increased the electron density around Au NPs, which was demonstrated to be an essential factor to the excellent catalytic activity of the Au-SnO/SiO composite. The simple and universal synthesis method afforded precise control over the size/spatial arrangement of Au and SnO NPs on SiO lamellas. The high activity of the Au-SnO/SiO composite demonstrated that the strategy used in this study has good potential application prospect. Furthermore, this work provided new perspective on the catalysis mechanism to the metal/semiconductor synergistic catalyst system.
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http://dx.doi.org/10.1021/acs.langmuir.0c03216DOI Listing
February 2021

Adsorption and desorption characteristics of polyphenols from Oliv. leaves with macroporous resin and its inhibitory effect on α-amylase and α-glucosidase.

Ann Transl Med 2020 Aug;8(16):1004

Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Institute of Analysis (China National Analytical Center Guangzhou), Guangdong Academy of Sciences, Guangzhou, China.

Background: Oliv. (EUO) was a traditional Chinese herb, its leaves were abundant in China, and polyphenol compounds were considered to be an important active ingredient in Oliv. leaves (EUOL). However, previous research mainly focused on compound identification and extraction process, there were few reported on the efficient enrichment process and biological activity evaluation of polyphenols in EUOL.

Methods: The adsorption and desorption characteristics of twelve different resins (HPD-100, HPD-300, HPD-600, D-3250, X-5, D-140, NKA-9, NKA-II, D-101, AB-8, S-8 and Polyamide) were investigated to develop an efficient method for the enrichment of polyphenol from EUOL, and the static adsorption, kinetics, isotherm and thermodynamics of the polyphenol from EUOL were analyzed. The eluted component was obtained through dynamic elution, and its main polyphenol compounds were detected by high-phase liquid chromatography (HPLC) and the inhibitory effects on the enzyme activity of α-amylase and α-glucosidase was also evaluated for different elution components. Meanwhile, the binding of main polyphenol compounds to enzyme was also evaluated.

Results: The selected resins (HPD-300, HPD-600, D-3250, X-5, D-140, NKA-9, D-101 and AB-8) showed adsorption patterns that fitted well to the pseudo second-order kinetics. The intra-particle diffusion model demonstrated that the diffusion of polyphenol compounds on these resins were divided into three processes. For HPD-300, HPD-600 and NKA-9, the Freundlich model better described the adsorption isotherm behavior than the Langmuir model, and the adsorption of polyphenol was a physical, exothermic, and spontaneous process. Subsequently, dynamic elution was performed yielding a higher polyphenol content in a 60% ethanol-water elution component, and it also exhibited a higher inhibitory effect on α-amylase and α-glucosidase activity. Furthermore, as the main polyphenol compounds, chlorogenic acid, rutin, quercetin and kaempferol were used to simulate the binding to the enzyme protein through molecular docking technology. The results showed that quercetin had a higher docking score for α-amylase, while rutin displayed superior binding to α-glucosidase.

Conclusions: Therefore, polyphenols of EUOL could be enriched through macroporous resins and have the potential to be effective enzyme inhibitor.
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http://dx.doi.org/10.21037/atm-20-5468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475476PMC
August 2020

Remarkable SERS Detection by Hybrid CuO/Ag Nanospheres.

ACS Omega 2020 Jul 7;5(28):17703-17714. Epub 2020 Jul 7.

Yunnan Key Laboratory of Opto-electronic Information Technology, Yunnan Normal University, Kunming 650500, P. R. China.

CuO nanospheres (NSs) were synthesized by modifying the glucose reduction method. Based on this method, CuO/Au (Ag) NSs were further prepared by in situ reduction of HAuCl (via electron beam evaporation of Ag). With Rhodamine 6G (R6G) as probe, the surface-enhanced Raman scattering (SERS) characteristics of the three samples were systematically studied. The experiment results showed that the enhancement factor (EF) of CuO/Au (Ag) NSs as 1.25 × 10 (2.74 × 10) and the ultralow detection limit (LOD) as 8.07 × 10 (1.13 × 10) M for R6G. The excellent performance of SERS may be due to the charge transfer (CT) between metal-semiconductor (MS) molecules and the strong electromagnetic field (E-field) of each hot spot. In addition, discrete dipole approximation (DDA) simulations were performed to simulate the E-field enhancement of the CuO and CuO/Au (Ag) NSs in a three-dimensional (3D) configuration. These further supported that the high SERS performance for R6G is because of the powerful E-field coupling between neighboring Au (Ag) NPs and the surface plasmon resonance (SPR) effect. The CuO/Ag NSs have potential in applications such as biomedicine, food safety, and environmental monitoring because of their high sensitivity and good reproducibility.
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http://dx.doi.org/10.1021/acsomega.0c02301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377325PMC
July 2020

Multifunctional Features of Organic Charge-Transfer Complexes: Advances and Perspectives.

Chemistry 2021 Jan 5;27(2):464-490. Epub 2020 Nov 5.

School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

The recent progress of charge-transfer complexes (CTCs) for application in many fields, such as charge transport, light emission, nonlinear optics, photoelectric conversion, and external stimuli response, makes them promising candidates for practical utility in pharmaceuticals, electronics, photonics, luminescence, sensors, molecular electronics and so on. Multicomponent CTCs have been gradually designed and prepared as novel organic active semiconductors with ideal performance and stability compared to single components. In this review, we mainly focus on the recently reported development of various charge-transfer complexes and their performance in field-effect transistors, light-emitting devices, lasers, sensors, and stimuli-responsive behaviors.
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http://dx.doi.org/10.1002/chem.202002640DOI Listing
January 2021

Use of Various Doses of S-Ketamine in Treatment of Depression and Pain in Cervical Carcinoma Patients with Mild/Moderate Depression After Laparoscopic Total Hysterectomy.

Med Sci Monit 2020 Jun 22;26:e922028. Epub 2020 Jun 22.

Department of Anesthesiology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine (TCM), Shanghai, China (mainland).

BACKGROUND This study investigated the effects of various doses of S-ketamine on depression and pain management of cervical carcinoma patients with mild/moderate depression. MATERIAL AND METHODS This randomized, double-blind, controlled study included 417 cervical carcinoma patients who received laparoscopic modified radical hysterectomy from April 2015 to July 2018 and who also had mild/moderate depression symptoms based on HAMD-17 scores (8~24). All patients were randomized into 4 groups: 1) the control group, 2) the racemic ketamine group, 3) the high-dose S-ketamine group; and 4) the low-dose S-ketamine group. Pain was assessed using the Visual Analogue Score (VAS), and depression was assessed using theHAMD-17 score. Serum levels of BDNF and 5-HT were measured. RESULTS The 4 groups of patients showed no significant differences in operation time, bleeding volume, hospitalization duration, or complications. The high-dose S-ketamine group showed significantly lower VAS and HAMD-17 scores than all other groups at 1 day and 3 days postoperatively, but no differences were observed in the low-dose S-ketamine group and the racemic ketamine group. The high-dose S-ketamine group showed significantly higher serum BDNF and 5-HT levels at 1 day and 3 days after surgery. However, 1 week after surgery, no difference was observed in any of the treatment groups. CONCLUSIONS At subanesthetic dose, both 0.5 mg/kg and 0.25 mg/kg S-ketamine improved short-term depression and pain for cervical carcinoma patients after surgery, and the effects were better than with the same dose of racemic ketamine.
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http://dx.doi.org/10.12659/MSM.922028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331479PMC
June 2020

Switchable Electromagnetically Induced Transparency with Toroidal Mode in a Graphene-Loaded All-Dielectric Metasurface.

Nanomaterials (Basel) 2020 May 30;10(6). Epub 2020 May 30.

National Laboratory of Solid State Microstructures, Key Laboratory of Intelligent Optical Sensing and Manipulation, Jiangsu Key Laboratory of Artificial Functional Materials, Collaborative Innovation Center of Advanced Microstructures, School of Physics and College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.

Active photonics based on graphene has attracted wide attention for developing tunable and compact optical devices with excellent performances. In this paper, the dynamic manipulation of electromagnetically induced transparency (EIT) with high quality factors (Q-factors) is realized in the optical telecommunication range via the graphene-loaded all-dielectric metasurface. The all-dielectric metasurface is composed of split Si nanocuboids, and high Q-factor EIT resonance stems from the destructive interference between the toroidal dipole resonance and the magnetic dipole resonance. As graphene is integrated on the all-dielectric metasurface, the modulation of the EIT window is realized by tuning the Fermi level of graphene, engendering an appreciable modulation depth of 88%. Moreover, the group velocity can be tuned from /1120 to /3390. Our proposed metasurface has the potential for optical filters, modulators, and switches.
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http://dx.doi.org/10.3390/nano10061064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352775PMC
May 2020

Effect of Pretreatment of S-Ketamine On Postoperative Depression for Breast Cancer Patients.

J Invest Surg 2020 Jan 16:1-6. Epub 2020 Jan 16.

Anesthesiology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China.

This study aims to investigate the effect of the pretreatment of S-ketamine on postoperative depression (POD) for breast cancer patients with mild/moderate depression. The present randomized, double-blinded controlled trial included 303 breast cancer patients with mild/moderate depression from June 2017 to June 2018. All patients were randomly divided into three groups: (1) control group, patients treated with normal saline; (2) racemic ketamine group, patients treated with racemic ketamine; (3) S-ketamine group, patients treated with S-ketamine. Operation time, blood loss and hospital stay and complications were recorded. The Visual Analog Scale (VAS) score was recorded, and the Hamilton Rating Scale for Depression (HAMD-17) scores, serum brain-derived neurotrophic factor (BDNF) and 5-hydroxytryptamine (5-HT) were measured at three days, one week, one month and three months after surgery. No significant difference was found in operation time, bleeding volume and complication rate. In both groups, the VAS scores at one day and three days after surgery were significantly lower. The HAMD-17 scores were significantly lower, and the serum levels of both BDNF and 5-HT were remarkably higher at three days, one week and one month after surgery. Meanwhile, the HAMD-17 scores were remarkably lower, while the serum levels of BDNF and 5-HT were remarkably higher in the S-ketamine group. The BDNF and 5-HT levels were negatively correlated with the HAMD-17 score. S-ketamine is more effective for reducing POD for breast cancer patients.
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http://dx.doi.org/10.1080/08941939.2019.1710626DOI Listing
January 2020

Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid.

J Integr Plant Biol 2020 Sep 26;62(9):1433-1451. Epub 2020 Feb 26.

College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.

Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms underlying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin biosynthesis in P. notoginseng. Acremonium sp. D212 could secrete indole-3-acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. notoginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2-15 μmol/L) and 1-naphthalenacetic acid (NAA) (10-20 μmol/L). Moreover, the roots of the JA signaling-defective coi1-18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild-type Nipponbare and miR393b-overexpressing lines, and the colonization was rescued by MeJA but not by NAA. It suggests that the cross-talk between JA signaling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants.
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http://dx.doi.org/10.1111/jipb.12905DOI Listing
September 2020

Cistanches alleviates sevoflurane-induced cognitive dysfunction by regulating PPAR-γ-dependent antioxidant and anti-inflammatory in rats.

J Cell Mol Med 2020 01 4;24(2):1345-1359. Epub 2019 Dec 4.

Department of Neurology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China.

This study aimed to investigate the protective effects and underlying mechanisms of cistanche on sevoflurane-induced aged cognitive dysfunction rat model. Aged (24 months) male SD rats were randomly assigned to four groups: control group, sevoflurane group, control + cistanche and sevoflurane + cistanche group. Subsequently, inflammatory cytokine levels were measured by ELISA, and the cognitive dysfunction of rats was evaluated by water maze test, open-field test and the fear conditioning test. Three days following anaesthesia, the rats were killed and hippocampus was harvested for the analysis of relative biomolecules. The oxidative stress level was indicated as nitrite and MDA concentration, along with the SOD and CAT activity. Finally, PPAR-γ antagonist was used to explore the mechanism of cistanche in vivo. The results showed that after inhaling the sevoflurane, 24- but not 3-month-old male SD rats developed obvious cognitive impairments in the behaviour test 3 days after anaesthesia. Intraperitoneal injection of cistanche at the dose of 50 mg/kg for 3 consecutive days before anaesthesia alleviated the sevoflurane-induced elevation of neuroinflammation levels and significantly attenuated the hippocampus-dependent memory impairments in 24-month-old rats. Cistanche also reduced the oxidative stress by decreasing nitrite and MDA while increasing the SOD and CAT activity. Moreover, such treatment also inhibited the activation of microglia. In addition, we demonstrated that PPAR-γ inhibition conversely alleviated cistanche-induced protective effect. Taken together, we demonstrated that cistanche can exert antioxidant, anti-inflammatory, anti-apoptosis and anti-activation of microglia effects on the development of sevoflurane-induced cognitive dysfunction by activating PPAR-γ signalling.
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http://dx.doi.org/10.1111/jcmm.14807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991648PMC
January 2020

Automatic Extraction of Structural and Non-Structural Road Edges from Mobile Laser Scanning Data.

Sensors (Basel) 2019 Nov 18;19(22). Epub 2019 Nov 18.

State Key Laboratory of Automotive Safety and Energy, School of Vehicle and Mobility, Tsinghua University, Beijing 100084, China.

Accurate road information is important for applications involving road maintenance, intelligent transportation, and road network updates. Mobile laser scanning (MLS) can effectively extract road information. However, accurately extracting road edges based on large-scale data for complex road conditions, including both structural and non-structural road types, remains difficult. In this study, a robust method to automatically extract structural and non-structural road edges based on a topological network of laser points between adjacent scan lines and auxiliary surfaces is proposed. The extraction of road and curb points was achieved mainly from the roughness of the extracted surface, without considering traditional thresholds (e.g., height jump, slope, and density). Five large-scale road datasets, containing different types of road curbs and complex road scenes, were used to evaluate the practicality, stability, and validity of the proposed method via qualitative and quantitative analyses. Measured values of the correctness, completeness, and quality of extracted road edges were over 95.5%, 91.7%, and 90.9%, respectively. These results confirm that the proposed method can extract road edges from large-scale MLS datasets without the need for auxiliary information on intensity, image, or geographic data. The proposed method is effective regardless of whether the road width is fixed, the road is regular, and the existence of pedestrians and vehicles. Most importantly, the proposed method provides a valuable solution for road edge extraction that is useful for road authorities when developing intelligent transportation systems, such as those required by self-driving vehicles.
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http://dx.doi.org/10.3390/s19225030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891364PMC
November 2019

Dietary supplementation with the extract from Eucommia ulmoides leaves changed epithelial restitution and gut microbial community and composition of weanling piglets.

PLoS One 2019 26;14(9):e0223002. Epub 2019 Sep 26.

Institute of Chemical Industry of Forest Products, CAF, Nanjing, P. R. China.

This study was conducted to compare the effects of Eucommia ulmoides leaves (EL) in different forms (EL extract, fermented EL, and EL powder) with antibiotics on growth performance, intestinal morphology, and the microbiota composition and diversity of weanling piglets. Compared to the control group, the antibiotics and EL extract significantly increased the average daily gain and decreased the feed: gain ratio as well as the diarrhea rate (P < 0.05). The EL extract significantly decreased the crypt depth and increased the ratio of villus height to crypt depth (P < 0.05), while the fermented EL group did the opposite (P < 0.05). The crypt depth in the antibiotics group was of similar value to the EL extract group, and was lower than the fermented EL and EL powder groups (P < 0.05). Compared to the control and antibiotics groups, the jejunul claudin-3 mRNA expression and the concentrations of total VFA, Chao 1, and ACE were significantly augmented in the EL extract group of piglets (P < 0.05). The EL extract groups also showed elevated Shannon (P < 0.05) and Simpson (P = 0.07) values relative to the control and antibiotics groups. At the phylum level, the EL extract group exhibited a reduced abundance of Bacteroidetes and an enhanced abundance of Firmicutes. At the genus level, the abundance of Prevotella was augmented in the EL extract group. Moreover, compared with the antibiotic group, the acetate concentration was enhanced in the EL extract and fermented EL groups. Overall, dietary supplementation with the EL extract, but not the fermented EL or EL powder, improved growth performance, jejunul morphology and function, as well as changed colonic microbial composition and diversity, which might be an alternative to confer protection against weanling stress in weanling piglets.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223002PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762056PMC
March 2020

RE-1 silencing transcription factor alleviates the growth-suppressive effects of propofol on mouse neuronal cells.

Neuroreport 2019 10;30(15):1025-1030

aDepartment of Anesthesiology, Seventh People's Hospital of Shanghai University of TCM bDepartment of Anesthesiology, Shanghai Songjiang District Central Hospital, Shanghai City, PR China.

Objective: Propofol is broadly utilized for maintaining anesthesia. Propofol affects neurodegeneration and neurogenesis by regulation of autophagy via effects on intracellular calcium homeostasis. The underlying molecular mechanism, however, is still unclear.

Methods: In the present research, we systematically analyzed the effect of propofol on mouse neuronal cells (cell line: HT-22). Cell Counting Kit-8 assays were utilized to examine cell proliferation. Flow cytometry was used to determine the levels of cell apoptosis. Quantitative real-time PCR and western blot were used to examine the relative mRNA and protein levels in mouse neuronal cells.

Results: Our results suggest that propofol inhibits proliferation and promotes apoptosis in neuronal cells. Moreover, overexpression of the transcriptional repressor RE-1 silencing transcription factor rescued the effect of propofol on neuronal cells. Additionally, the autophagy inhibitor 3-methyladenine also significantly reduced the effect of propofol on mouse neuronal cells. Finally, overexpression of RE-1 silencing transcription factor promoted the expression of brain-derived neurotrophic factor in mouse neuronal cells.

Conclusion: Our research not only enhances our understanding of propofol on mouse neuronal cells but also uncovers a potential signaling pathway that may mediate the effects of propofol on neuronal cells.
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http://dx.doi.org/10.1097/WNR.0000000000001321DOI Listing
October 2019

Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction.

J Int Med Res 2019 Aug 16;47(8):3860-3873. Epub 2019 Jul 16.

1 Department of Anaesthesiology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China.

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http://dx.doi.org/10.1177/0300060519860026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726816PMC
August 2019

The short interference RNA (siRNA) targeting NMUR2 relieves nociception in a bone cancer pain model of rat through PKC-ERK and PI3K-AKT pathways.

Biochem Biophys Res Commun 2019 05 23;512(3):616-622. Epub 2019 Mar 23.

Department of Anesthesiology, Seventh People's Hospital of Shanghai University of TCM, No.358 Datong Road, Shanghai, 200137, China.

Background/aim: Bone cancer pain (BCP) causes troubles and burdens to patients globally. Increasing evidence proved that neuromedin U receptor 2 (NMUR2) was involved in pains. Our study was performed to investigate the role of NMUR2 on BCP and the underlying mechanism.

Methods: The rats were raised and BCP rat model was established by injection with Walker 256 cells. The RNA and protein expression levels of NMUR2 in rat neurons-dorsal spinal cord cells, RNdsc cells were detected by qRT-PCR and western blot. The administration with NMUR2 was via intrathecal injection with siRNA to silence NMUR2. The tolerance of rat to pain was measured by mechanical allodynia test and presented by paw withdrawal threshold (PWT) value. The effects on protein kinase C (PKC)/extracellular regulated protein kinases (ERK) and phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signal pathways were examined by western blot.

Results: The expression of NMUR2 in both mRNA and protein levels was upregulated in BCP rat model. In addition, siRNA injection significantly decreased the expression of NMUR2 on the 3rd, 7th and 14th day. BCP group revealed lower PWT value compared with control while NMUR2 silence increased the PWT value compared with negative control. The phosphorylation of PKC, ERK, PI3K and AKT was increased in BCP model while was decreased by si-NMUR2. PKC/ERK and PI3K/AKT inhibitor administration increased the PWT value compared with BCP group.

Conclusion: si-NMUR2 alleviates BCP via inactivation of PKC/ERK and PI3K/AKT signal pathways.
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http://dx.doi.org/10.1016/j.bbrc.2019.03.067DOI Listing
May 2019

Optimal geometry parameter for plasmonic sensitivities of individual Au nanopoarticle sensors.

Phys Chem Chem Phys 2019 Apr;21(14):7654-7660

College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 211100, China.

The shape, aspect ratio and size are key parameters governing the plasmon sensitivities of individual Au nanoparticle bio/chemical sensors. It is crucial to unveil the general geometry parameters to optimize their corresponding sensitivity applications. In this work, the geometry-dependent refractive-index sensitivity factor (S) and figure of merit (FOM) of individual Au nanoparticle sensors (including a nanodisc, nanorod, nanoellipsoid and hexagonal nanoplate) are numerically investigated by discrete dipole approximation (DDA). S is revealed to increase quadratically/linearly with aspect ratio, while FOM reaches a maximum at an optimized aspect ratio of about 3.0/8.0 for the studied prolate/oblate nanoparticles, respectively, reflecting their shapes and aspect ratios and, hence, their size effects. However, their responses to shape factors are shown to follow nearly the same trend regardless of their different detailed geometries, demonstrating that their shape factors provide the general geometry parameters governing the plasmon sensitivities of the concerned individual Au nanoparticle sensors. This can be analytically explained well under dipolar localized surface plasmon resonance (LSPR) conditions. Their optimal FOM is predicted to be about 12.5 RIU-1 at a shape factor of 10.5; the underlying reason for this is analytically discussed as well. The obtained results in this work are believed to hold great promise for choosing appropriate nanoparticle geometry parameters for individual Au nanoparticle LSPR-based bio/chemical sensor design and applications as well as to access the corresponding optimal geometry parameters and FOM simultaneously.
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http://dx.doi.org/10.1039/c9cp00309fDOI Listing
April 2019

[Preparation of hydrophilic, magnetic molecularly imprinted resins of chlorogenic acid and evaluation of its solid-phase extraction performance].

Se Pu 2019 Mar;37(3):293-298

Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China.

Hydrophilic, magnetic molecularly imprinted resins (MMIRs) were fabricated inside FeO@mSiO pores by the one-pot copolycondensation of chlorogenic acid (CGA), resorcinol, melamine, and formaldehyde. The final porous MMIRs with larger numbers of surface recognition sites were obtained by removing mSiO. The successful preparation of MMIRs was confirmed using transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermo-gravimetric analysis, and water contact angle analysis. The analyses suggest that the MMIRs have excellent hydrophilicity and magnetic solid-phase extraction efficiency. The MMIRs had a good adsorption capacity (50.87 mg/g), fast rate of adsorption (equilibrium was attained at 70 min), and specific recognition for CGA. The MMIRs, in combination with high performance liquid chromatography, were directly used for the selective extraction and determination of CGA in Duzhong brick tea. The limit of detection and recovery were 0.7 mg/L and 93.1%-109.4%, respectively, with relative standard deviations of less than 9.6%. The proposed strategy was extremely promising for the facile, rapid, and selective determination of CGA in real aqueous samples.
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http://dx.doi.org/10.3724/SP.J.1123.2018.11026DOI Listing
March 2019

Emerging porous materials in confined spaces: from chromatographic applications to flow chemistry.

Chem Soc Rev 2019 May;48(9):2566-2595

Sun Yat-Sen University, MOE Laboratory of Polymeric Composite and Functional Materials, Guangzhou 510275, China.

Searching for porous materials that can be employed as solid stationary phases for chromatographic separations, porous membrane matrixes and solid supports for catalysis has become an active research area. Strategies for embedding emerging porous materials in columnar systems and their subsequent applications (separation and catalysis) have been developed, which benefit from the remarkable progress in the discovery and development of porous materials based on metal-organic coordination or dynamic covalent bonding such as metal-organic frameworks (MOFs), covalent-organic frameworks (COFs), porous organic cages, and porous organic polymers. In this review, porous materials that have been confined within capillary columns as packed, monolithic and open tubular columns are discussed. Progress in chromatographic separation and continuous flow catalytic synthesis is reviewed according to three major strategies. Applications of porous materials in membrane-separation fibre membrane systems and microfluidic devices with various functions are also highlighted.
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http://dx.doi.org/10.1039/c8cs00657aDOI Listing
May 2019

Investigation of Self-Assembly and Charge-Transport Property of One-dimensional PDI₈-CN₂ Nanowires by Solvent-Vapor Annealing.

Materials (Basel) 2019 Jan 31;12(3). Epub 2019 Jan 31.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

One-dimensional (1D) nanowires have attracted great interest, while air-stable -type 1D nanowires still remain scarce. Herein, we present solvent-vapor annealing (SVA) made nanowires based on perylene tetracarboxylic diimide (PDI) derivative. It was found that the spin-coated thin films reorganized into nanowires distributed all over the substrate, as a result of the following solvent-vapor annealing effect. Cooperating with the atomic force microscopy and fluorescence microscopy characterization, the PDI₈-CN₂ molecules were supposed to conduct a long-range and entire transport to form the 1D nanowires through the SVA process, which may guarantee its potential morphology tailoring ability. In addition, the nanowire-based transistors displayed air stable electron mobility reaching to 0.15 cm² V s, attributing to effective in situ reassembly. Owing to the broader application of organic small-molecule nanowires, this work opens up an attractive approach for exploring new high-performance micro- and nanoelectronics.
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http://dx.doi.org/10.3390/ma12030438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384653PMC
January 2019

pH-Responsive Oxygen Nanobubbles for Spontaneous Oxygen Delivery in Hypoxic Tumors.

Langmuir 2019 08 12;35(31):10166-10172. Epub 2019 Feb 12.

Tumor hypoxia is a significant factor leading to the resistance of tumors to treatment, especially for photodynamic therapy and radiotherapy where oxygen is needed to kill cancer cells. Oxygen delivery agents such as oxygen-saturated perfluorocarbon nanoemulsions and lipid oxygen microbubbles have been employed to supply oxygen to hypoxic tumors with ultrasound activation. Such oxygen delivery systems are still associated with several drawbacks, including premature oxygen release and the dependence of external stimuli. To address these limitations, we developed oxygen nanobubbles that were enclosed by the acetalated dextran polymer shells for spontaneous oxygeneration in response to a minor pH drop in the tumor microenvironment. The acetalated dextran polymer shell serves as a robust barrier against gas dissolution in the circulating blood to retain the majority of the oxygen payload, and its pH-responsive property enables an abrupt burst release of oxygen in the mild acidic tumor microenvironment. The acetalated dextran oxygen nanobubbles exhibited excellent stability and biocompatibility. In vitro and in vivo experiments were conducted to investigate the pH-responsive oxygen release. The external stimuli-free supply of oxygen by the acetalated dextran oxygen nanobubbles was evaluated on CNE2 tumor-bearing mice, and the intratumoral oxygen level increased by 6-fold after the administration of the oxygen nanobubbles, manifesting that our pH-responsive oxygen nanobubbles hold great potential as a potent oxygen delivery agent to overcome the hypoxia-induced resistance.
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http://dx.doi.org/10.1021/acs.langmuir.8b03650DOI Listing
August 2019

Isolation of polyphenol compounds from olive waste and inhibition of their derivatives for -glucosidase and -amylase.

Nat Prod Res 2020 Aug 2;34(16):2398-2402. Epub 2019 Jan 2.

Institute of Chemical Industry of Forest Products, CAF, Nanjing, China.

Olive waste was used as a sustainable resource because it contained a variety of valuable compounds. The polyphenols active fraction from enrichment by microporous resin and extraction with ethyl acetate were analysed by different chromatographic methods. A total of 14 polyphenolic compounds were isolated and identified by structure elucidation. Based on the above obtained compounds, tyrosol was selected as a characteristic polyphenol and participated in transesterification reaction to synthesise -ketoester using Yb(OTf). Then the Biginelli reaction with benzaldehyde, urea and ketoester (1:1.2:1.2) was performed at 90 °C for 3.0 h under the acidic condition. In addition, the -ketoester prepared using tyrosol with benzyl had a greater inhibitory effect on -glucosidase and -amylase, and the inhibition of enzyme activity for 3, 4-dihydropyrimidinone derivatives prepared using abovementioned -ketoester was improved significantly. Meanwhile, fluorine-containing dihydropyrimidinone derivatives were considerable inhibitors for both enzymes.
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http://dx.doi.org/10.1080/14786419.2018.1538217DOI Listing
August 2020

Dysfunctional endothelial-derived microparticles promote inflammatory macrophage formation via NF-кB and IL-1β signal pathways.

J Cell Mol Med 2019 01 18;23(1):476-486. Epub 2018 Oct 18.

Key Laboratory of Arrhythmias of the Ministry of Education of China, Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Circulating endothelial-derived microparticles (EMPs) are reported to be increased in acute coronary syndrome (ACS). However, it remains unclear whether EMPs from dysfunctional endothelium participate in the initiation and progression of ACS and what the underlying mechanisms might be.

Methods: Plasma EMPs were measured in 22 patients with ACS and 20 control patients without coronary artery diseases. EMPs from dysfunctional human umbilical vein endothelial cells (HUVECs) stressed by serum-starvation or hypoxia were compared to the EMPs from healthy HUVECs. Confocal and fluorescent microscopy was used to visualize the incorporation of EMPs into monocytes and the translocation of NF-кB. Monocyte adhesion, cell proliferation, and phagocytosis were detected by PKH26 red fluorescent labelling, Ki67 immunostaining, and Sudan IV staining for uptake of oxidized low-density lipoprotein, respectively.

Results: Plasma EMPs was significantly increased in ACS patients compared to controls. EMPs were incorporated into monocytes and EMPs from stressed HUVECs produced more pro-inflammatory cytokines compared to vehicle control, which was depended on NF-кB and IL-1β signal pathways. EMPs from dysfunctional endothelium promoted monocyte adherence via NF-кB and IL-1β-mediated MCP-1 and CCR-5 signals, as well as proliferation via the NF-кB and IL-1β-mediated Cyclin D1 signals. Finally, EMPs from dysfunctional endothelium showed greater promotion of macrophage phagocytosis forming foam cells to produce more pro-inflammatory cytokines.

Conclusion: MPs might be involved in the inflammatory process in patients with ACS via NF-κB and IL-1β-dependent signals. Targeting EMP-mediated inflammatory responses may be a promising therapeutic strategy to limit the progression of disease in ACS.
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http://dx.doi.org/10.1111/jcmm.13950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307808PMC
January 2019

[Mechanism of reduced insecticidal protein expression in Bt cotton under high temperature and drought based on proteomics.]

Ying Yong Sheng Tai Xue Bao 2018 Aug;29(8):2590-2600

Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, China.

To provide theoretical basis for the safety of insecticidal efficiency in Bt cotton, the effects of high temperature and drought stress on insecticidal protein expression and protein diffe-rently expression profile was studied. In this study, the Bt cotton cultivar Sikang 3 was used as expe-rimental material, with two treatments (40% field capacity and 38 ℃, HD, and 60% field capacity and 32 ℃, CK). Differences in proteomics of Bt cotton between HD and CK were compared using label-free quantitative proteomics technology. The results showed that high temperature and drought caused a significant reduction of insecticidal protein content in bolls, with a decrease of 38.2 ng·g FM. The analysis of differential protein expression by label-free quantitative proteomic approach showed that 83 proteins were significantly up-regulated, but 104 proteins were significantly down-regulated in HD stressed cotton plants compared with CK. 122 new proteins were detected and 167 proteins expression was not observed under stressed conditions. Results from the enrichment analysis of differently expressed protein between two treatments showed that 14 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were affected under stress. Three KEGG pathways were related to the Bt protein synthesis and degradation: carbohydrate digestion and absorption pathway, protein export pathway, and protein processing in endoplasmic reticulum pathway. In the carbohydrate digestion and absorption pathway, the starch hydrolysis ability of HD treated cotton plants increased, while the ability to phosphorylate the hexoses, fructose and glucose decreased. In protein export pathway, the peptide synthesis in HD treatment was not significantly affected, while the process of transferring peptides into the endoplasmic reticulum was prohibited. In the protein processing in endoplasmic reticulum pathway, the ability of ubiquitin mediated proteolysis was increased in HD treatment.
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http://dx.doi.org/10.13287/j.1001-9332.201808.006DOI Listing
August 2018

Applying Regional Citrate Anticoagulation in Continuous Renal Replacement Therapy for Acute Kidney Injury Patients with Acute Liver Dysfunction: a Retrospective Observational Study.

Kidney Blood Press Res 2018 3;43(4):1065-1074. Epub 2018 Jul 3.

Background/aims: Continuous renal replacement therapy (CRRT) is a treatment for acute kidney injury (AKI) patients. It has become a controversy about whether patients with liver dysfunction should perform CRRT with regional citrate anticoagulation (RCA).

Methods: This retrospective observational study enrolled 145 AKI patients (275 CRRT sessions) who received CRRT with RCA and had no history of chronic liver disease. Circuit survival time, blood pressure, trans-membrane pressure (TMP), acid-base and electrolyte status were recorded and analyzed. The severity of liver dysfunction was determined by total bilirubin (TBil) and international normalized ratio (INR), while the accumulation degree of citrates was quantified by total/ ionized calcium (tCa/iCa) raito.

Results: Our results showed that there was no correlation of tCa/iCa ratio with TBil or INR. And tCa/iCa ratio was not related to the disturbances of pH, lactates, sodium, magnesium, blood pressure or TMP despite that high tCa/iCa ratios might be related to the decrease of circuit survival time. TBil did not correlate with the above indexes, except for lactates levels. INR did not correlate with the above indexes except for lactates levels and blood pressure. In addition, neither was TBil, INR, nor tCa/iCa ratio, related with fatal outcomes (22.76% of the patients).

Conclusion: The present study demonstrated that, with proper monitoring and adjustment of citrates and calcium infusion, applying RCA in CRRT is reasonably safe for AKI patients with acute liver dysfunction, as long as circuit time stays below roughly 50 hours.
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http://dx.doi.org/10.1159/000491057DOI Listing
November 2018

[Mixed amanita phalloides poisoning with rhabdomyolysis: analysis of 4 cases].

Nan Fang Yi Ke Da Xue Xue Bao 2018 May;38(5):635-638

Intensive Care Unit, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail:

We report the clinical characteristics, treatments and outcomes of 4 rare cases of mixed amanita fuliginea and amanita rimosa poisoning with rhabdomyolysis, and review the research progress in the intoxication mechanism and treatment. The latent time of amanita poisoning, defined as the period from the ingestion of poisonous mushroom to the onset of gastrointestinal symptoms, was about 8 days, and the severity of poisoning was associated with the amount of mushroom ingested. All the 4 patients developed multiple organ dysfunctions within 3 to 4 days after mushroom ingestion, predominantly in the liver, kidney and central nervous system accompanied with acute gastrointestinal injury and rhabdomyolysis. The treatment measures included persistent hemofiltration and intermittent hemoperfusion once daily for 5-7 days, and plasma exchange was administered in 2 cases for 1 or 2 times. High-dose vitamin C, glucose and corticosteroid were also given to the patients. After the treatments, two patients were cured and the other two died due to an excess intake of poisonous mushroom and lack of early preemptive therapies. Early emetic, gastric lavage, catharsis, fluid infusion and diuresis are critical to interrupt the enterohepatic circulation of amanita phalloides toxins and prevent the development of multiple organ dysfunction. Enhanced hemofiltration and sequential plasma therapy might effectively eliminate toxin from the blood to protect against further organ damages.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743900PMC
May 2018

Elevated Expression of miR302-367 in Endothelial Cells Inhibits Developmental Angiogenesis via CDC42/CCND1 Mediated Signaling Pathways.

Theranostics 2018 5;8(6):1511-1526. Epub 2018 Feb 5.

Key Laboratory of Arrhythmias of the Ministry of Education of China, Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.

Angiogenesis is critical for embryonic development and microRNAs fine-tune this process, but the underlying mechanisms remain incompletely understood. Endothelial cell (EC) specific miR302-367 line was used as gain-of-function and anti-miRs as loss-of-function models to investigate the effects of miR302-367 on developmental angiogenesis with embryonic hindbrain vasculature as an model and fibrin gel beads and tube formation assay as models. Cell migration was evaluated by Boyden chamber and scratch wound healing assay and cell proliferation by cell count, MTT assay, Ki67 immunostaining and PI cell cycle analysis. RNA high-throughput sequencing identified miR-target genes confirmed by chromatin immunoprecipitation and 3'-UTR luciferase reporter assay, and finally target site blocker determined the pathway contributing significantly to the phenotype observed upon microRNA expression. Elevated EC miR302-367 expression reduced developmental angiogenesis, whereas it was enhanced by inhibition of miR302-367, possibly due to the intrinsic inhibitory effects on EC migration and proliferation. We identified Cdc42 as a direct target gene and elevated EC miR302-367 decreased total and active Cdc42, and further inhibited F-actin formation via the WASP and Klf2/Grb2/Pak1/LIM-kinase/Cofilin pathways. MiR302-367-mediated-Klf2 regulation of Grb2 for fine-tuning Pak1 activation contributing to the inhibited F-actin formation, and then the attenuation of EC migration. Moreover, miR302-367 directly down-regulated EC Ccnd1 and impaired cell proliferation via the Rb/E2F pathway. miR302-367 regulation of endothelial Cdc42 and Ccnd1 signal pathways for EC migration and proliferation advances our understanding of developmental angiogenesis, and meanwhile provides a rationale for future interventions of pathological angiogenesis that shares many common features of physiological angiogenesis.
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http://dx.doi.org/10.7150/thno.21986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858164PMC
January 2019