Publications by authors named "Sheng Liu"

1,022 Publications

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Outcomes between in-hospital stroke and community-onset stroke after thrombectomy: Propensity-score matching analysis.

Interv Neuroradiol 2021 Sep 13:15910199211030769. Epub 2021 Sep 13.

Department of Interventional Radiology, 74734The First Affiliated Hospital with Nanjing Medical University, China.

Background: The benefit of endovascular thrombectomy for patients with in-hospital stroke remains unclear. Thus, the aim of this study was to compare the endovascular thrombectomy outcomes between in-hospital stroke and community-onset stroke among patients with acute ischemic stroke.

Methods: From January 2015 to July 2019, 362 consecutive patients with acute ischemic stroke with large vessel occlusion in the anterior circulation received endovascular thrombectomy in our centre. After propensity score matching with a ratio of 1:2 (in-hospital stroke:community-onset stroke), clinical characteristics and functional outcomes were compared between in-hospital stroke and community-onset stroke groups.

Results: Thirty-six patients with in-hospital stroke and 72 patients with community-onset stroke were enrolled. The number of patients with New York Heart Association classification III/IV (41.7% vs. 6.9%,  < 0.001) and with underlying cancer (25.0% vs. 2.8%, < 0.001) was higher in the in-hospital stroke than in the community-onset stroke group. The intravenous thrombolysis rate was lower in the in-hospital stroke group (13.9% vs. 43.1%,  = 0.002). No significant difference in symptom onset to puncture ( = 0.618), symptom onset to recanalisation ( = 0.618) or good reperfusion (modified thrombolysis in cerebral infarction ≥2) rates ( = 0.852) was found between the groups. The favourable clinical outcome trend (modified Rankin scale ≤2 at 90 days) was inferior, but acceptable, in the in-hospital stroke, group compared to the community-onset stroke group (30.6% vs. 41.7%,  = 0.262).

Conclusion: Patients with in-hospital stroke had more disadvantageous comorbidities than those with community-onset stroke. Cardiac dysfunction seems to be associated with poor outcomes after thrombectomy. Nevertheless, endovascular thrombectomy still appears to be safe and effective for patients with in-hospital stroke.
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http://dx.doi.org/10.1177/15910199211030769DOI Listing
September 2021

Direct Observation of Magnon-Phonon Strong Coupling in Two-Dimensional Antiferromagnet at High Magnetic Fields.

Phys Rev Lett 2021 Aug;127(9):097401

State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics, Tsinghua University, Beijing 100084, China.

We report the direct observation of strong coupling between magnons and phonons in a two-dimensional antiferromagnetic semiconductor FePS_{3}, via magneto-Raman spectroscopy at magnetic fields up to 30 Tesla. A Raman-active magnon at 121  cm^{-1} is identified through Zeeman splitting in an applied magnetic field. At a field-driven resonance with a nearby phonon mode, a hybridized magnon-phonon quasiparticle is formed due to strong coupling between the two modes. We develop a microscopic model of the strong coupling in the two-dimensional magnetic lattice, which enables us to elucidate the nature of the emergent quasiparticle. Our polarized Raman results directly show that the magnons transfer their spin angular momentum to the phonons and generate phonon spin through the strong coupling.
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http://dx.doi.org/10.1103/PhysRevLett.127.097401DOI Listing
August 2021

Application Value of Mathematical Models of Diffusion-Weighted Magnetic Resonance Imaging in Differentiating Breast Cancer Lesions.

Evid Based Complement Alternat Med 2021 28;2021:1481271. Epub 2021 Aug 28.

The Affiliated Nanhua Hospital, Department of Radiology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.

Objective: To determine the application value of the mono-exponential model, dual-exponential model, and stretched-exponential model of MRI with diffusion-weighted imaging (DWI) in breast cancer (BC) lesions.

Methods: Totally 64 cases with BC admitted to our hospital between June 2019 and October 2020 were enrolled in this study. They had 71 lesions in total, including 40 benign tumor lesions (including 9 breast cyst lesions) and 31 malignant tumor lesions. After DWI examination, with normal glands as control, mono-exponential model (ADC) map, dual-exponential model (Standard-ADC) map, slow apparent diffusion coefficient (Slow-ADC) map, fast-apparent diffusion coefficient (Fast-ADC) map, and stretched-exponential model (DDC) map were processed, and corresponding values were generated. Then, the situation and significance of each parameter in breast cysts, benign breast tumor lesions, and malignant tumor lesions were analyzed.

Results: The values of ADC, Standard-ADC, and DDC of breast cysts were higher than those of normal glands (all < 0.05), and the values of ADC and DDC of benign breast tumor lesions were lower than those of normal glands ( < 0.05). In addition, malignant breast tumor lesions had lower values of ADC, Standard-ADC, Slow-ADC, and DDC and a higher Fast-ADC value compared to normal glands (all < 0.05). Compared with benign tumor lesions, malignant tumor lesions had lower values of ADC, Standard-ADC, Slow-ADC, and DDC and a higher value of Fast-ADC (all < 0.05). Moreover, the receiver operating characteristic (ROC) curve-based analysis revealed that all the above models could be adopted to effectively evaluate the deterioration of benign breast tumor lesions (all < 0.05), and DDC value had the most significant diagnostic effect on malignant tumor lesions ( < 0.05).

Conclusion: Both dual-exponential model and stretched-exponential model of DWI can help effectively evaluate the progression of benign breast tumors, and the stretched-exponential model is more effective in the diagnosis of malignant breast tumors. These models are of great help to the future clinical diagnosis of BC.
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http://dx.doi.org/10.1155/2021/1481271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421181PMC
August 2021

Mediator Subunit16 Induces BnMED25- and BnWRKY33-Activated Defense Signaling to Confer Resistance.

Front Plant Sci 2021 19;12:663536. Epub 2021 Aug 19.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, China.

The plant mediator is a highly conserved protein complex that interacts with transcription factors (TFs) and RNA polymerase II (RNAP II) to relay regulatory information during transcription. Plant immune response is one of the biological processes that is orchestrated by this regulatory mechanism. s, an important oil crop, is severely attacked by a devastating disease Sclerotinia stem rot. Here, we explored broad-spectrum disease resistant roles of mediator subunit 16 (BnMED16) and its host defense mechanism against fugal pathogen . We found that expression was significantly increased by infection, and its homologous overexpression resulted in rapid and comprehensive defense responses from the beginning to the end. This affected signal transduction with multiple channels including pathogen recognition, intracellular Ca concentration, reactive oxygen species (ROS) accumulation and clearance, and activation of mitogen-activated protein kinase (MAPK) signaling cascades initially. Subsequently, pathogen-/defense-related genes and hormone-responsive pathways were highly activated, which resulted in enhanced cell wall and secretion of defense proteases. Furthermore, the biochemical analysis showed that BnMED16 interacts with BnMED25 and BnWRKY33. Additionally, BnMED25 also interacts with TFs BnMYC2, BnCOI1, and BnEIN3 of the JA/ET signal transduction pathway. Taken together, we proposed a hypothetical model that BnMED16 confers resistance by enhancing BnMED25-mediated JA/ET defense pathways and BnWRKY33-activated defense signaling in . The overexpressing lines with enhanced broad-spectrum disease resistance could be useful for breeding -resistant oilseed rape varieties, as well as serving as basis for further strategy development in resistance breeding.
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http://dx.doi.org/10.3389/fpls.2021.663536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417128PMC
August 2021

miR-195-5p regulates cell proliferation, apoptosis, and invasion of thyroid cancer by targeting telomerase reverse transcriptase.

Bioengineered 2021 Dec;12(1):6201-6209

Department Of Head And Neck Surgery, Jiangxi Provincial Cancer Hospital, Jiangxi Cancer Hospital of Nanchang University, Jiangxi Provincial Key Laboratory of Translational Medicine and Oncology, Jiangxi Cancer Center, Nanchang, Jiangxi, China.

In most human primary cancers, the expression, or telomerase activity, of telomerase reverse transcriptase () is detectable. However, the mechanism ofactivity within oncogenesis of thyroid cancer remains largely unknown. In this study, we identified miR-195-5p as having involvement in cell proliferation, apoptosis, and invasion in human thyroid cancer. MTT was used to measure cell proliferation, Transwell chamber was used to measure invasion. Western blotting was used to detect the expressions of TERT, PCNA, and Ki67. Target gene prediction software predicted that TERT may be the target gene of miR-195-5p. Luciferase reporting system was used to identify the targeting relationship. A significant increase of in TERT expression was observed by immunohistochemistry compared with normal tissue, however, a decrease in miR-195-5p expression using qRT-PCRand western blot compared with normal cells. Functional analysis demonstrates that miR-195-5p negatively correlated withand inhibitedexpression through its interaction with the3'-untranslatedregion (3'-UTR). Overexpression of miR-195-5p was shown to inhibit proliferation and invasion, and promote apoptosis of CAL-62 thyroid cancer cells. miR-195-5p-mediatedeffects were rescued by the overexpression of. Altogether, our data demonstrate that miR-195-5p regulates cell proliferation, apoptosis, and invasion in human thyroid cancer via, providing evidence of a new potential therapeutic target for further investigation.
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http://dx.doi.org/10.1080/21655979.2021.1963908DOI Listing
December 2021

Juvenile hormone and transcriptional changes in honey bee worker larvae when exposed to sublethal concentrations of thiamethoxam.

Ecotoxicol Environ Saf 2021 Sep 1;225:112744. Epub 2021 Sep 1.

Key Laboratory of Agricultural Environment and Agricultural Product Safety, Guangxi University, China. Electronic address:

Thiamethoxam, an insecticide with high usage and large amounts of environmental residues, has been reported to affect the pupation and survival of honey bee larvae at sublethal concentrations. The molecular mechanisms are not fully understood. In this study, we measured the response of juvenile hormone (JH) to environmental concentrations of thiamethoxam using liquid chromatography-tandem mass spectrometry (LC-MS/MS), monitored the dynamic changes in the transcription of genes encoding major JH metabolic enzymes (CYP15A1, FAMET, JHAMT and JHE) using RT-qPCR, and analysed the transcriptome changes in worker larvae under thiamethoxam stress using RNA-seq. Thiamethoxam significantly increased the levels of JH3 in honey bee larvae, but no significant changes in the transcript levels of the four major metabolic enzymes were observed. Thiamethoxam exposure resulted in 140 differentially expressed genes (DEGs). P450 CYP6AS5 was upregulated, and some ion-related, odourant-related and gustatory receptors for sugar taste genes were altered significantly. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that amino acid metabolism and protein digestion and absorption were influenced by thiamethoxam. These changes may do harm to honey bee caste differentiation, foraging behaviour related to sensory perception and nutrient levels of bee colonies. These results represent the first assessment of the effects of thiamethoxam on JH in honey bee larvae and provides a new perspective and molecular basis for the study of JH regulation and thiamethoxam toxicity to honey bees.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112744DOI Listing
September 2021

Surveillance strategy for complete ablation of initial recurrent hepatocellular carcinoma: a risk-based machine learning study.

J Vasc Interv Radiol 2021 Aug 30. Epub 2021 Aug 30.

Department of Medical Imaging and Interventional Radiology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China. Electronic address:

Purpose: For initial recurrent hepatocellular carcinoma (irHCC), there is no consensus regarding the monitoring strategy after ablation. The present study investigated surveillance strategies for irHCC after ablation to support clinical decision making.

Materials And Methods: Data from irHCC patients who received ablation were retrospectively collected at two medical centers. The risk of tumor relapse in each month was calculated through random survival forest methodology, and follow-up schedules were arranged thereafter to maximize the capability of relapse detection at each visit.

Results: The cumulative 0.5-, 1-, 1.5-, and 2-year risk-adjusted probabilities in the train/validation cohorts were 26.2%/21.5%, 42.3%/39.4%, 55.5%/52.6%, and 61.3%/63.2%, respectively, with the highest recurrence rate occurring in the second month (maximum= 7.9%/7.4%). The surveillance regime primarily concentrated on the first year post treatment, especially the initial six months. The delay in detecting tumor recurrence gradually decreased when the total number of follow-up visits increased from 4 to 8. Compared with the control strategies, this schedule (follow-up visits at 2, 4, 6, 9, 12, and 18 months) reduced the delay in detection. The benefits of this surveillance regime were evident when the patients were followed up six times. The proposed 6-visit surveillance strategy significantly decreased the delay in detection compared with the control 7-visit approach (1.32 versus 1.82 months, P< 0.001).

Conclusion: The proposed new surveillance schedule minimized the delay in detecting recurrence in irHCC patients after ablation. Our risk-related machine learning method could be applied to develop follow-up strategies for other HCC patients.
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http://dx.doi.org/10.1016/j.jvir.2021.07.025DOI Listing
August 2021

ADGRG1 enriches for functional human hematopoietic stem cells following ex vivo expansion-induced mitochondrial oxidative stress.

J Clin Invest 2021 Aug 31. Epub 2021 Aug 31.

Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The heterogeneity of human hematopoietic stem (HSCs) and progenitor cells (HPCs) under stress conditions such as ex vivo expansion is poorly understood. Here we report that the frequencies of SCID repopulating cells (SRCs) were greatly decreased in cord blood(CB)CD34+ HSCs and HPCs upon ex vivo culture. Transcriptome analysis and metabolic profiling demonstrated that mitochondrial oxidative stress of human CB HSCs and HPCs notably increased along with loss of stemness. Limiting dilution analysis (LDA) revealed that functional human HSCs were enriched in cell populations with low levels of mitochondrial reactive oxygen species (mitoROS) during ex vivo culture. Using single cell RNA sequencing (scRNA-seq) analysis of mitoROS low cell population, we demonstrated that functional HSCs were substantially enriched in the Adhesion G protein-coupled receptor G1 positive (ADGRG1+) population of CD34+CD133+ CB cells upon ex vivo expansion stress. GSEA analysis revealed that HSC signature genes including MSI2 and MLLT3 are enriched in CD34+CD133+ ADGRG1+ CB HSCs. Our study reveals that ADGRG1 enriches for functional human HSCs under oxidative stress during ex vivo culture, which can be a reliable target for drug screening of agonists of HSC expansion.
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http://dx.doi.org/10.1172/JCI148329DOI Listing
August 2021

Osthole inhibits the migration and invasion of highly metastatic breast cancer cells by suppressing ITGα3/ITGβ5 signaling.

Acta Pharmacol Sin 2021 Aug 23. Epub 2021 Aug 23.

Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.

Metastasis is the leading cause of death in breast cancer patients. Osthole, as an active compound detected in the traditional Chinese medicine Wenshen Zhuanggu Formula, has shown a promising anti-metastatic activity in human breast cancer cells, but the underlying mechanisms remain ambiguous. In this study we elucidated the anti-metastatic mechanisms of osthole in highly metastatic breast cancer cells and a zebrafish xenograft model. We showed that the expression of integrin α3 (ITGα3) and integrin β5 (ITGβ5) was upregulated in highly metastatic MDA-MB-231, MDA-MB-231BO breast cancer cell lines but was downregulated in poorly metastatic MCF-7 breast cancer cell line, which might be the key targets of osthole's anti-metastatic action. Furthermore, we showed that knockdown of ITGα3 and ITGβ5 attenuated breast cancer cell migration and invasion possibly via suppression of FAK/Src/Rac1 pathway, whereas overexpression of ITGα3 and ITGβ5 caused the opposite effects. Consistently, osthole significantly inhibited breast cancer metastasis by downregulating ITGα3/ITGβ5 signaling in vitro and in vivo. These results provide new evidence that osthole may be developed as a candidate therapeutic drug for metastatic breast cancer.
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http://dx.doi.org/10.1038/s41401-021-00757-7DOI Listing
August 2021

SIRT6 controls hepatic lipogenesis by suppressing LXR, ChREBP, and SREBP1.

Biochim Biophys Acta Mol Basis Dis 2021 Aug 21;1867(12):166249. Epub 2021 Aug 21.

Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA; Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address:

Fatty liver disease is the most prevalent chronic liver disorder, which is manifested by hepatic triglyceride elevation, inflammation, and fibrosis. Sirtuin 6 (Sirt6), an NAD-dependent deacetylase, has been implicated in hepatic glucose and lipid metabolism; however, the underlying mechanisms are incompletely understood. The aim of this study was to identify and characterize novel players and mechanisms that are responsible for the Sirt6-mediated metabolic regulation in the liver. We generated and characterized Sirt6 liver-specific knockout mice regarding its role in the development of fatty liver disease. We used cell models to validate the molecular alterations observed in the animal models. Biochemical and molecular biological approaches were used to illustrate protein-protein interactions and gene regulation. Our data show that Sirt6 liver-specific knockout mice develop more severe fatty liver disease than wild-type mice do on a Western diet. Hepatic Sirt6 deficiency leads to elevated levels and transcriptional activities of carbohydrate response element binding protein (ChREBP) and sterol regulatory element binding protein 1 (SREBP1). Mechanistically, our data reveal protein-protein interactions between Sirt6 and liver X receptor α (LXRα), ChREBP, or SREBP1c in hepatocytes. Moreover, Sirt6 suppresses transcriptional activities of LXRα, ChREBP, and SREBP1c through direct deacetylation. In conclusion, this work has identified a key mechanism that is responsible for the salutary function of Sirt6 in the inhibition of hepatic lipogenesis by suppressing LXR, ChREBP, and SREBP1.
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http://dx.doi.org/10.1016/j.bbadis.2021.166249DOI Listing
August 2021

Grafting and Depositing Lithium Polysulfides on Cathodes for Cycling Stability of Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):40685-40694. Epub 2021 Aug 19.

Institute of New Energy Material Chemistry, School of Materials Science and Engineering, Nankai University, Tianjin 300350, China.

Based on dissolution/deposition chemistry, together with multielectron redox reactions, lithium-sulfur (Li-S) batteries have been demonstrated as a promising energy storage system. However, the diffusion of soluble lithium polysulfide intermediates (LiPSs) to bulk electrolyte results in the fast capacity fade of a Li-S cell. How to confine the LiPSs within the cathode while retaining high reversible capacity remains a huge challenge. In this work, -bromophthalimide, an organic molecule with an aromatic heterocyclic ring and a reactive halogen bond, is introduced as an electrolyte additive to conquer the excessive dissolution and diffusion of LiPSs by in situ formation of an organopolysulfide deposition layer. This electrochemically active layer not only maintains the internal sulfur conversion but also prevents LiPSs from diffusing into the electrolyte bulk, thereby improving the cycling and rate performance of Li-S batteries. This study provides a feasible strategy for regulating the reaction region and path for high-performance Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c11904DOI Listing
September 2021

Molecular Basis of Taste and Micronutrient Content in Kumamoto Oysters () and Portuguese Oysters () From Xiangshan Bay.

Front Physiol 2021 27;12:713736. Epub 2021 Jul 27.

Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo, China.

Oysters are the most extensively cultivated bivalves globally. Kumamoto oysters, which are sympatric with Portuguese oysters in Xiangshan bay, China, are regarded as particularly tasty. However, the molecular basis of their characteristic taste has not been identified yet. In the present study, the taste and micronutrient content of the two oyster species were compared. Portuguese oysters were larger and had a greater proportion of proteins (48.2 ± 1.6%), but Kumamoto oysters contained significantly more glycogen (21.8 ± 2.1%; < 0.05). Moisture and lipid content did not differ significantly between the two species ( > 0.05). Kumamoto oysters contained more Ca, Cu, and Zn ( < 0.05); whereas Mg and Fe levels were comparable ( > 0.05). Similarly, there was no significant difference between the two species with respect to total amount of free amino acids, umami and bitterness amino acids, succinic acid (SA), and most flavoring nucleotides ( > 0.05). In contrast, sweetness amino acids were significantly more abundant in Portuguese oysters. Volatile organic compounds profiles of the two species revealed a higher proportion of most aldehydes including (2,4)-hepta-2,4-dienal in Kumamoto oysters. Overall, Kumamoto oysters contain abundant glycogen, Ca, Zn, and Cu, as well as volatile organic compounds, especially aldehydes, which may contribute to their special taste. However, free amino acid and flavor nucleotides may not the source of special taste of Kumamoto oyster. These results provide the molecular basis for understanding the characteristic taste of Kumamoto oysters and for utilizing local oyster germplasm resources.
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http://dx.doi.org/10.3389/fphys.2021.713736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353274PMC
July 2021

Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.

Mol Cell 2021 Sep 9;81(17):3560-3575.e6. Epub 2021 Aug 9.

Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA; Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. Electronic address:

Transcription initiation by RNA polymerase II (RNA Pol II) requires preinitiation complex (PIC) assembly at gene promoters. In the dynamic nucleus, where thousands of promoters are broadly distributed in chromatin, it is unclear how multiple individual components converge on any target to establish the PIC. Here we use live-cell, single-molecule tracking in S. cerevisiae to visualize constrained exploration of the nucleoplasm by PIC components and Mediator's key role in guiding this process. On chromatin, TFIID/TATA-binding protein (TBP), Mediator, and RNA Pol II instruct assembly of a short-lived PIC, which occurs infrequently but efficiently within a few seconds on average. Moreover, PIC exclusion by nucleosome encroachment underscores regulated promoter accessibility by chromatin remodeling. Thus, coordinated nuclear exploration and recruitment to accessible targets underlies dynamic PIC establishment in yeast. Our study provides a global spatiotemporal model for transcription initiation in live cells.
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http://dx.doi.org/10.1016/j.molcel.2021.07.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420877PMC
September 2021

Microbial characteristics across different tongue coating types in a healthy population.

J Oral Microbiol 2021 26;13(1):1946316. Epub 2021 Jul 26.

Departments of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

The physical appearance of tongue coatings is vital for traditional Chinese medicine (TCM) to diagnose health and disease status. The microbiota of different tongue coatings could also influence coating formation and be further associated with specific diseases. Previous studies have focused on bacteria from different tongue coatings in the context of specific diseases, but the normal variations in healthy individuals remain unknown. We examined the tongue microbiota by metagenomics in 94 healthy individuals classified into eight different tongue types. The overall composition of the tongue coating microbiome is not drastically different among different coating types, similar to the findings of previous studies in healthy populations. Further analysis revealed microbiota characteristics of each coating type, and many of the key bacteria are reported to be implicated in diseases. Moreover, further inclusion of diabetic patients revealed disease-specific enrichment of , even though the same tongue coatings were studied. This work revealed the characteristic compositions of distinctive tongue coatings in a healthy population, which serves as a basis for understanding the tongue coating formation mechanism and provides a valuable reference to further investigate disease-specific tongue coating bacterial markers.
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http://dx.doi.org/10.1080/20002297.2021.1946316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317956PMC
July 2021

Full-Color Holographic Display and Encryption with Full-Polarization Degree of Freedom.

Adv Mater 2021 Aug 7:e2103192. Epub 2021 Aug 7.

MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions, Shaanxi Key Laboratory of Optical Information Technology, School of Physical Science and Technology, Northwestern Polytechnical University, Xi'an, 710129, China.

Metasurfaces provide a compact and powerful platform for manipulating the fundamental properties of light, and have shown unprecedented capabilities in both optical holographic display and information encryption. For increasing information display/storage capacity, metasurfaces with more polarization manipulation channel and full-color holographic functionality are now an urgent requirement. Here, a minimalist dielectric metasurface with the capability of full-color holography encoded with arbitrary polarization is proposed and experimentally demonstrated. Without the daunting exploratory and computational problem in nanostructure searching, full-color holographic images can be multiplexed into arbitrary polarization channels through vectorial ptychography and k-space ptychography based on tetratomic macropixel geometric phase metasurfaces. Thanks to the full degree of freedom tuning in polarization and color spaces, the application scenarios such as holographic 3D imaging and information encryption are realized. The strategy exhibits promising potential in applications of 3Dl display, augmented/virtual reality, high-density data storage, and encryption.
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http://dx.doi.org/10.1002/adma.202103192DOI Listing
August 2021

Box lesion or bi-atrial lesion set for atrial fibrillation during thoracoscopic epicardial ablation.

Interact Cardiovasc Thorac Surg 2021 Aug 3. Epub 2021 Aug 3.

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objectives: Thoracoscopic epicardial ablation with a limited lesion set led to suboptimal results for advanced paroxysmal atrial fibrillation (AF) or persistent AF. Whether additional right atrial lesions improve the result is unclear.

Methods: We conducted a retrospective study involving 80 consecutive patients with paroxysmal or persistent AF, left atrial (LA) dilation (LA diameter >40 mm) and failed prior interventional ablation (40 patients, 50%) who underwent thoracoscopic epicardial ablation with box lesions (36 patients) or bi-atrial (BA) lesion (44 patients) in our institution. Freedom from atrial tachyarrhythmias after the procedures was compared between the box lesion group and BA lesion group.

Results: Baseline differences included more patients with persistent AF (86.4% vs 47.2%) and larger left atrium [48.00 (44.00-50.75) vs 42.00 (41.25-44.00) mm] in the BA lesion group. There was no difference in procedural complications between the 2 groups. After a mean follow-up of 32 months, the freedom from atrial tachyarrhythmias off antiarrhythmic drugs at 6, 12 and 24 months was 77.2%, 77.2% and 77.2% in the BA lesion group and 69.4%, 50.0% and 40.6% in the box lesion group, respectively (P = 0.006). After adjustment for sex, age, body mass index, LA diameter, AF type, history of AF, and previous interventional ablation, BA lesion was an independent predictor of lower atrial tachyarrhythmia recurrence (hazard ratio 0.447, 95% confidential interval 0.208-0.963; P = 0.040).

Conclusions: Compared with the box lesion set, thoracoscopic epicardial ablation with BA lesion sets might provide better freedom from atrial tachyarrhythmias for paroxysmal or persistent AF with LA dilation. Randomized control trials are warranted to confirm the benefit of BA lesion sets in these patients.
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http://dx.doi.org/10.1093/icvts/ivab211DOI Listing
August 2021

Anthropogenic disturbances on antibiotic resistome along the Yarlung Tsangpo River on the Tibetan Plateau: Ecological dissemination mechanisms of antibiotic resistance genes to bacterial pathogens.

Water Res 2021 Sep 20;202:117447. Epub 2021 Jul 20.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.

Human activities can accelerate the antibiotic resistome prevalence and pose threats to ecological safety and public health globally. However, antibiotic resistance gene (ARG) mobility and dissemination into bacterial pathogens under anthropogenic disturbances are still poorly understood. Here, we used a metagenomic approach to profile the biogeography of ARGs and pathogenic antibiotic resistant bacteria (PARB) under anthropogenic disturbances along the Yarlung Tsangpo River. Results showed the ARGs was dominated by bacA gene along the Yarlung Tsangpo River on the Tibetan Plateau. The ARG composition was differently impacted by rapid urbanization and dam construction, which urbanization could promote ARGs resistant to sulfonamide and tetracycline, whereas dam construction could elevate the resistance to chloramphenicol and aminoglycoside. Land use pattern was identified as a critical factor influencing ARG composition under anthropogenic disturbances, as it could directly reflect the land degradation level and indicate the inputs of ARG-selective chemicals of different human activities. Moreover, despite of the lack of variation in ARG relative abundance, PARB were highly promoted by anthropogenic activities, indicating increasing ARG dissemination to pathogen. We found that human-impacted environments harbored high proportion of mobile genetic elements (MGEs), and the MGE carrying ARGs also increased under anthropogenic disturbances in the pathogenic hosts, which confirmed that anthropogenic activities could promote ARG horizontal gene transfer. Furthermore, anthropogenic activities could influence PARB assembly processes. Basically, stochastic processes dominated PARB assembly along the river, and with increasing level of anthropogenic activities, these processes shifted from undominated stochastic processes to dispersal limitation. In summary, this study provides useful strategies in watershed resistome management and reduction of ARG dissemination to pathogens, which should consider the mode and intensity of human activity and its potential influence on horizontal gene transfer and assembly processes.
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http://dx.doi.org/10.1016/j.watres.2021.117447DOI Listing
September 2021

Simultaneous hybrid maze procedure for long-standing persistent atrial fibrillation with dilated atrium.

JTCVS Tech 2021 Feb 27;5:34-42. Epub 2020 Oct 27.

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The efficacy of catheter-based or thoracoscopic ablation for treating long-standing persistent atrial fibrillation (AF) with a dilated atrium remains suboptimal. This study aimed to assess the feasibility and initial results of simultaneous hybrid ablation with a new biatrial lesion set in these patients.

Methods: Twenty-seven consecutive patients with long-standing persistent AF and dilated atrium underwent simultaneous hybrid ablation with a new biatrial lesion set consistent with the principle of the Cox maze procedure at our institution. Patients were followed up at 3, 6, and 12 months after the procedure and annually thereafter.

Results: After thoracoscopic epicardial ablation, endocardial mapping confirmed complete bilateral pulmonary vein isolation in 25 patients (96.2%). All patients were followed for a mean of 18.7 ± 8.9 months. Freedom from atrial tachyarrhythmia at 1 year after the single hybrid procedure was 64% with antiarrhythmic drugs and 60% without antiarrhythmic drugs. Freedom from atrial tachyarrhythmia at latest follow-up after the hybrid procedure and redo catheter ablation was 77.8% with antiarrhythmic drugs and 74.1% without antiarrhythmic drugs. In patients with sinus rhythm restoration, mean left atrial diameter decreased from 54.4 ± 4.3 mm to 45.2 ± 4.1 mm ( < .001), and mean right atrial diameter decreased from 59.4 ± 3.9 mm to 54.9 ± 4.4 mm ( < .001). Postoperative pleural effusion was observed in 3 patients (11.1%), and no adverse events occurred during follow-up.

Conclusions: In patients with long-standing persistent AF and a dilated atrium, simultaneous hybrid ablation with the new biatrial lesion set, in combination with touch-up catheter ablation when necessary, can achieve promising results. More studies are needed to confirm these findings.
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http://dx.doi.org/10.1016/j.xjtc.2020.10.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300042PMC
February 2021

Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin.

Elife 2021 07 27;10. Epub 2021 Jul 27.

Department of Biology, Johns Hopkins University, Baltimore, United States.

Conserved ATP-dependent chromatin remodelers establish and maintain genome-wide chromatin architectures of regulatory DNA during cellular lifespan, but the temporal interactions between remodelers and chromatin targets have been obscure. We performed live-cell single-molecule tracking for RSC, SWI/SNF, CHD1, ISW1, ISW2, and INO80 remodeling complexes in budding yeast and detected hyperkinetic behaviors for chromatin-bound molecules that frequently transition to the free state for all complexes. Chromatin-bound remodelers display notably higher diffusion than nucleosomal histones, and strikingly fast dissociation kinetics with 4-7 s mean residence times. These enhanced dynamics require ATP binding or hydrolysis by the catalytic ATPase, uncovering an additional function to its established role in nucleosome remodeling. Kinetic simulations show that multiple remodelers can repeatedly occupy the same promoter region on a timescale of minutes, implicating an unending 'tug-of-war' that controls a temporally shifting window of accessibility for the transcription initiation machinery.
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http://dx.doi.org/10.7554/eLife.69387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352589PMC
July 2021

Chiral Phonons and Giant Magneto-Optical Effect in CrBr 2D Magnet.

Adv Mater 2021 Sep 24;33(36):e2101618. Epub 2021 Jul 24.

State Key Laboratory of Low-Dimensional Quantum Physics and Department of Physics, Tsinghua University, Beijing, 100084, P. R. China.

Phonons with chirality determine the optical helicity of inelastic light scattering processes due to their nonzero angular momentum. Here it is shown that 2D magnetic CrBr hosts chiral phonons at the Brillouin-zone center. These chiral phonons are linear combinations of the doubly-degenerate E phonons, and the phonon eigenmodes exhibit clockwise and counterclockwise rotational vibrations corresponding to angular momenta of l = ± 1. Such E chiral phonons completely switch the polarization of incident circularly polarized light. On the other hand, the non-degenerate non-chiral A phonons display a giant magneto-optical effect under an external out-of-plane magnetic field, rotating the plane of polarization of the scattered linearly polarized light. The corresponding degree of polarization of the scattered light changes from 91% to -68% as the magnetic field strength increases from 0 to 5 T. In contrast, the chiral E modes display no field dependence. The results lay a foundation for the study of phonon chirality and magneto-optical phenomena in 2D magnetic materials, as well as their related applications, such as the phonon Hall effect, topological photonics, and Raman lasing.
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http://dx.doi.org/10.1002/adma.202101618DOI Listing
September 2021

Layer-engineered interlayer excitons.

Sci Adv 2021 Jul 23;7(30). Epub 2021 Jul 23.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.

Photoluminescence (PL) from excitons serves as a powerful tool to characterize the optoelectronic property and band structure of semiconductors, especially for atomically thin two-dimensional transition metal dichalcogenide (TMD) materials. However, PL quenches quickly when the thickness of TMD materials increases from monolayer to a few layers, due to the change from direct to indirect band transition. Here, we show that PL can be recovered by engineering multilayer heterostructures, with the band transition reserved to be a direct type. We report emission from layer-engineered interlayer excitons from these multilayer heterostructures. Moreover, as desired for valleytronics devices, the lifetime, valley polarization, and valley lifetime of the generated interlayer excitons can all be substantially improved as compared with that in the monolayer-monolayer heterostructure. Our results pave the way for controlling the properties of interlayer excitons by layer engineering.
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http://dx.doi.org/10.1126/sciadv.abh0863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302131PMC
July 2021

A Nomogram to Predict Recurrence After Bronchial Artery Embolization for Hemoptysis Due to Bronchiectasis.

Cardiovasc Intervent Radiol 2021 Jul 19. Epub 2021 Jul 19.

Department of Interventional Radiology, The First Affiliated Hospital With Nanjing Medical University, No. 300 Guangzhou Road, Nanjing, 210029, China.

Objective: To develop and validate a nomogram for predicting recurrent hemoptysis after successful bronchial arterial embolization (BAE) in patients with bronchiectasis.

Materials And Methods: From January 2015 to December 2019, a total of 251 patients were enrolled in this study. A nomogram was developed with the predictors of recurrent events, which were identified by univariate and multivariate Cox regression analyses. We evaluated nomogram discrimination by area under the receiver operating characteristic curve, calibration by the calibration curve, and clinical usefulness potential by decision curve analysis.

Results: The one-month, 1-year, 2-year, 3-year, and 5-year cumulative recurrence-free rates of patients were 98.4%, 90.5%, 82.8%, 77.7%, and 74.4%, respectively. Three predictive factors, namely sex, lung destruction, and systemic arterial-pulmonary circulation shunts, were applied to develop the nomogram. The model maintained good discrimination (area under the curve, 0.72; 95% confidence interval, 0.62-0.81), low prediction error (integrated Brier score, 0.129), and certain net benefits in terms of clinical usefulness.

Conclusions: The proposed nomogram showed favorable predictive efficacy for hemoptysis recurrence after BAE in patients with bronchiectasis. Improved long-term outcomes are expected with close follow-up, a healthy lifestyle, and pulmonary rehabilitation for patients at risk of recurrence according to the model.
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http://dx.doi.org/10.1007/s00270-021-02923-0DOI Listing
July 2021

Congener Substitution Reinforced LiPSbSO Glass-Ceramic Electrolytes for All-Solid-State Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2021 Jul 18;13(29):34477-34485. Epub 2021 Jul 18.

Institute of New Energy Material Chemistry, School of Materials Science and Engineering, Nankai University, Tianjin 300350, China.

Glass-ceramic sulfide solid electrolytes like LiPS are practicable propellants for safe and high-performance all-solid-state lithium-sulfur batteries (ASSLSBs); however, the stability and conductivity issues remain unsatisfactory. Herein, we propose a congener substitution strategy to optimize LiPS as LiPSbSO via chemical bond and structure regulation. Specifically, LiPSbSO is obtained by a SbO dopant to achieve partial Sb/P and O/S substitution. Benefiting from the strengthened oxysulfide structural unit of POS and POS with bridging oxygen atoms and a distorted lattice configuration of the Sb-S tetrahedron, the LiPSbSO electrolyte exhibits prominent chemical stability and high ionic conductivity. Besides the improved air stability, the ionic conductivity of LiPSbSO could reach 1.61 × 10 S cm at room temperature with a wide electrochemical window of up to 5 V (vs Li/Li), as well as good stability against Li and Li-In alloy anodes. Consequently, the ASSLSB with the LiPSbSO electrolyte shows high discharge capacities of 1374.4 mAh g (0.05C, 50th cycle) at room temperature and 1365.4 mAh g (0.1C, 100th cycle) at 60 °C. The battery also presents remarkable rate performance (1158.3 mAh g at 1C) and high Coulombic efficiency (>99.8%). This work provides a feasible technical route for fabricating ASSLSBs.
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http://dx.doi.org/10.1021/acsami.1c10238DOI Listing
July 2021

Ecological insights into the disturbances in bacterioplankton communities due to emerging organic pollutants from different anthropogenic activities along an urban river.

Sci Total Environ 2021 Nov 10;796:148973. Epub 2021 Jul 10.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.

Emerging organic pollutants (EOPs) in urban rivers have raised concerns regarding their eco-toxicological effects. However, the bacterioplankton community disturbances caused by EOPs in urban rivers and the associated ecological mechanisms remain unclear. This study provided profiles of the spatial distribution of a bacterioplankton community disturbed by human activity along an urban river. The results showed that EOP concentration and composition were differently distributed in residential and industrial areas, which significantly influenced bacterioplankton community structure. Based on redundancy analysis, parabens (methylparaben and propylparaben) were the major factors driving bacterioplankton community changes. Parabens inhibited gram-positive bacteria and promoted oxidative stress-tolerant bacteria in the river ecosystem. Parabens also disturbed ecological processes of bacterioplankton community assembly, shifting from a homogeneous selection (consistent selection pressure under similar environmental condition) to stochastic processes (random changes due to birth, death, immigration, and emigration) with changing in paraben concentrations. Heterogeneous selection was predicted to dominate microbial community assembly with paraben concentration changes exceeding 61.6 ng/L, which could deteriorate the river ecosystem. Furthermore, specific bacterial genera were identified as potential bioindicators to assess the condition of EOP contaminants in the river. Overall, this study highlights significant disturbances in bacterioplankton communities by EOPs at environmental concentrations, and our results could facilitate generation of appropriate management strategies aimed at EOPs in urban rivers.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148973DOI Listing
November 2021

Electroacupuncture Attenuates Anxiety-Like Behaviors in a Rat Model of Post-traumatic Stress Disorder: The Role of the Ventromedial Prefrontal Cortex.

Front Neurosci 2021 24;15:690159. Epub 2021 Jun 24.

Department of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Electroacupuncture (EA) is a promising clinical approach to treating posttraumatic stress disorder (PTSD), yet the mechanisms whereby EA can alleviate anxiety and other PTSD symptoms have yet to be clarified. In the present report, rats underwent EA for 14 consecutive days following modified single prolonged stress (MSPS) exposure. These animals were then evaluated in open field and elevated plus maze tests (OFT and EPM), while Fos immunohistochemical staining was performed to assess ventromedial prefrontal cortex (vmPFC) functional activation. In addition, an extracellular recording and stimulation system was used to analyze vmPFC inputs into the ventral tegmental area (VTA) in these rats. Temporary vmPFC inactivation was further performed to assess whether this was sufficient to reverse the anxiolytic effects of EA. Overall, rats that underwent EA treatment spent more time in the central region (OFT) and the open arm (EPM) relative to MSPS model animals ( < 0.05). These MSPS model animals also exhibited significantly fewer activated Fos-positive nuclei in the vmPFC following behavioral testing, while EA was associated with a significant relative increase in c-Fos expression in this region. The transient inactivation of the vmPFC was sufficient to reverse the effects of EA treatment on anxiety-like behaviors in MSPS model rats. MSPS and SEA rats exhibiting no differences in bursting activity between baseline and vmPFC stimulation, whereas bursting activity rose relative to baseline upon ventral mPFC stimulation in EA treated and control rats. Together, these findings indicate that the vmPFC and its inputs into the VTA are functionally linked to the anxiolytic activity of EA, implicating this pathway in the EA-mediated treatment of PTSD.
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http://dx.doi.org/10.3389/fnins.2021.690159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264195PMC
June 2021

Ruyiping extract reduces lung metastasis in triple negative breast cancer by regulating macrophage polarization.

Biomed Pharmacother 2021 Sep 9;141:111883. Epub 2021 Jul 9.

Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. Electronic address:

Lung metastasis of Triple-negative breast cancer (TNBC) causes severe breath-related events and poor prognosis. Ruyiping (RYP), a traditional Chinese medicine prescription, is used to treat breast cancer lung metastasis in clinical practice. This study was to explore the anti-lung-metastatic activities and mechanism of RYP extract by regulating macrophage polarization. The results showed that RYP can inhibit the viability and induce the apoptosis of TNBC cells. In in vitro experiments, RYP significantly inhibited the invasion and migration ability of TNBC cells promoted by M2, the subtype of macrophage which increased TNBC metastasis related genes. In in vivo experiments, RYP reduced the TNBC progression and lung metastasis. M2/M1 ration in the lung and M2 in the tumor was reduced by RYP, as well as M2 master regulator Stat6. Therefore, RYP extract may exhibit anti-lung metastasis function by reducing M2 in both tumor and lung through reducing Stat6.
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http://dx.doi.org/10.1016/j.biopha.2021.111883DOI Listing
September 2021

Updated SARS-CoV-2 single nucleotide variants and mortality association.

J Med Virol 2021 Jul 10. Epub 2021 Jul 10.

Department of BioHealth Informatics, Indiana University School of Informatics and Computing, Indiana University - Purdue University Indianapolis, Indianapolis, Indiana, USA.

By analyzing newly collected SARS-CoV-2 genomes and comparing them with our previous study about SARS-CoV-2 single nucleotide variants (SNVs) before June 2020, we found that the SNV clustering had changed remarkably since June 2020. Apart from that the group of SNVs became dominant, which is represented by two nonsynonymous mutations A23403G (S:D614G) and C14408T (ORF1ab:P4715L), a few emerging groups of SNVs were recognized with sharply increased monthly incidence ratios of up to 70% in November 2020. Further investigation revealed sets of SNVs specific to patients' ages and/or gender, or strongly associated with mortality. Our logistic regression model explored features contributing to mortality status, including three critical SNVs, G25088T(S:V1176F), T27484C (ORF7a:L31L), and T25A (upstream of ORF1ab), ages above 40 years old, and the male gender. The protein structure analysis indicated that the emerging subgroups of nonsynonymous SNVs and the mortality-related ones were located on the protein surface area. The clashes in protein structure introduced by these mutations might in turn affect the viral pathogenesis through the alteration of protein conformation, leading to a difference in transmission and virulence. Particularly, we explored the fact that nonsynonymous SNVs tended to occur in intrinsic disordered regions of Spike and ORF1ab to significantly increase hydrophobicity, suggesting a potential role in the change of protein folding related to immune evasion.
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http://dx.doi.org/10.1002/jmv.27191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426680PMC
July 2021

Does green credit affect the green innovation performance of high-polluting and energy-intensive enterprises? Evidence from a quasi-natural experiment.

Environ Sci Pollut Res Int 2021 Jul 6. Epub 2021 Jul 6.

School of Economics and Trade, Guangdong University of Finance, Guangzhou, 510521, Guangdong, China.

Taking the green credit policy in 2012 as a quasi-natural experiment, this paper applies the methods of propensity score matching and Difference-in-Difference (PSM-DID) to investigate the relationship between green credit policy and enterprises' green technology innovation performance based on Chinese industrial enterprises database and green patent database. The results show that the implementation of "green credit guidelines" policy has significantly improved the green innovation performance of high-polluting and high-energy consuming enterprises, which indicates that the incentive effect of green credit policy on enterprises exceeds the constraint effect and leads to "Porter effect." Moreover, the green credit policy has significantly increased the number of non-invention patents rather than invention patents. In addition, the green credit policy has a more significant effect on the green innovation performance of high-polluting and energy-intensive enterprises that are state-owned and have weak market power. Mechanism test shows that green credit policy can change the credit financing constraints and R&D investment allocation to affect the green innovation performance of high-polluting and energy-intensive enterprises.
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http://dx.doi.org/10.1007/s11356-021-15217-2DOI Listing
July 2021

Ruyiping formula inhibits metastasis via the microRNA-134-SLUG axis in breast cancer.

BMC Complement Med Ther 2021 Jul 5;21(1):191. Epub 2021 Jul 5.

Institute of Chinese Traditional Surgery, LongHua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 725 Wanpingnan Road, Shanghai, 200032, China.

Background: Metastasis is the leading cause of death among breast cancer patients. MicroRNA-134 has been reported to have a tumor-suppressive role in breast cancer. Ruyiping (RYP), a traditional Chinese formula, has been shown with the ability to reduce breast cancer metastasis in pre-clinical studies. This present study was designed to examine whether miR-134 was involved in RYP-inhibited breast cancer metastasis.

Methods: The expression of SLUG, E-Cadherin, N-Cadherin and miR-134 in MDA-MB-231 and 4 T1 cells treated with RYP or vehicle control were determined by quantitative realtime-PCR and western blot. Invasiveness determined by transwell assay as well as SLUG gene expression determined by qPCR were detected in cells transfected with chemically synthesized miR-134 mimics or inhibitors. BALB/c mice were injected with 4 T1 cells orthotopically and fed with RYP through gavage. Breast tumor growth, metastasis and tumor expression of EMT markers were detected.

Results: Compared with the control, Ruyiping formula significantly inhibited SLUG-regulated breast cancer cells invasion. MiR-134 was induced by RYP in vitro and in vivo and was able to suppress SLUG by targeting its 3'UTR. RYP suppressed SLUG expression and cell invasion through miR-134. In 4 T1 tumor-bearing mice, RYP significantly inhibited 4 T1 tumor growth and lung metastasis, increased the levels of miR-134 and epithelial marker while decreased the levels of SLUG and mesenchymal marker.

Conclusion: Our data uncovered that Ruyiping formula exerts an anti-metastatic activity against breast cancer cells by regulating SLUG through miR-134. MiR-134-SLUG axis might be a promising strategy in breast cancer therapy.
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http://dx.doi.org/10.1186/s12906-021-03365-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258945PMC
July 2021

Psychological Distress and Trust in University Management Among International Students During the COVID-19 Pandemic.

Front Psychol 2021 18;12:679661. Epub 2021 Jun 18.

School of Finance, Zhongnan University of Economics and Law, Wuhan, China.

Since the end of 2019, the outbreak of the COVID-19 pandemic has engendered widespread fear and anxiety across China. Nearly half a million international students pursuing their studies in Chinese universities have also been exposed to the psychological distress triggered by the unfolding crisis. In addition to government and medical institutions' efforts, universities have also endeavored to mitigate concerns among these students under quarantine on campus by providing reliable information as well as medical, monetary, and emotional support. In this study, international students' trust in university management teams and its role in remediating their anxieties were evaluated using an online survey conducted after 10 days of the lockdown of Wuhan, China. The empirical analysis incorporates quantitative data from 180 international students. Ordinary least squares regression and probit regression were used in the analysis with the non-robust and robust models. The study found students' perception of trust in university management to be negatively associated with their anxiety levels. Additionally, having trust in university management was found to positively influence students' commitment to the self-quarantine guidelines. These results reinforce the important role of universities and their relationship with international students during public health emergencies.
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http://dx.doi.org/10.3389/fpsyg.2021.679661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250427PMC
June 2021
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