Publications by authors named "Shen Liu"

234 Publications

Fiber-tip polymer clamped-beam probe for high-sensitivity nanoforce measurements.

Light Sci Appl 2021 Aug 27;10(1):171. Epub 2021 Aug 27.

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education/GuangDong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China.

Micromanipulation and biological, material science, and medical applications often require to control or measure the forces asserted on small objects. Here, we demonstrate for the first time the microprinting of a novel fiber-tip-polymer clamped-beam probe micro-force sensor for the examination of biological samples. The proposed sensor consists of two bases, a clamped beam, and a force-sensing probe, which were developed using a femtosecond-laser-induced two-photon polymerization (TPP) technique. Based on the finite element method (FEM), the static performance of the structure was simulated to provide the basis for the structural design. A miniature all-fiber micro-force sensor of this type exhibited an ultrahigh force sensitivity of 1.51 nm μN, a detection limit of 54.9 nN, and an unambiguous sensor measurement range of ~2.9 mN. The Young's modulus of polydimethylsiloxane, a butterfly feeler, and human hair were successfully measured with the proposed sensor. To the best of our knowledge, this fiber sensor has the smallest force-detection limit in direct contact mode reported to date, comparable to that of an atomic force microscope (AFM). This approach opens new avenues towards the realization of small-footprint AFMs that could be easily adapted for use in outside specialized laboratories. As such, we believe that this device will be beneficial for high-precision biomedical and material science examination, and the proposed fabrication method provides a new route for the next generation of research on complex fiber-integrated polymer devices.
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http://dx.doi.org/10.1038/s41377-021-00611-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397746PMC
August 2021

shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus.

Sci Rep 2021 Aug 12;11(1):16377. Epub 2021 Aug 12.

State Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.

Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV (P < 0.05). Twenty-three transgenic cloned swine (TGCS) and nine non-transgenic cloned swine (Non-TGCS) were produced by somatic cell nuclear transfer (SCNT). In the FMDV challenge study, one TGCS was completely protected, no clinical signs, no viremia and no viral RNA in the tissues, no non-structural antibody response, another one TGCS swine recovered after showing clinical signs for two days, whereas all of the normal control swine (NS) and Non-TGCS developed typical clinical signs, viremia and viral RNA was determined in the tissues, the non-structural antibody was determined, and one Non-TGCS swine died. The viral RNA load in the blood and tissues of the TGCS was reduced in both challenge doses. These results indicated that the TGCS displayed resistance to the FMDV infection. Immune cells, including CD3, CD4, CD8, CD21, and CD172 cells, and the production of IFN-γ were analyzed, there were no significant differences observed between the TGCS and NS or Non-TGCS, suggesting that the FMDV resistance may be mainly derived from the RNAi-based antiviral pathway. Our work provides a foundation for a breeding approach to preventing infectious disease in swine.
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http://dx.doi.org/10.1038/s41598-021-95853-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361160PMC
August 2021

[Review of Computer Modeling and Simulation of Spinal Thermal Ablation].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Jul;45(4):416-419

Department of Engineering Physics, Tsinghua University, Beijing, 100091.

Thermal ablation surgery can effectively eliminate bone tumors in the spine and meanwhile reduce damage to the human body. To realize the computer modeling and simulation of spine thermal ablation surgery, it is necessary to ensure the accuracy of both spine modeling and simulation temperature. This review summarizes the research progress of this field and analyzes the prospects from two aspects: computer modeling based on spine segmentation from medical images and simulation calculation of temperature field in ablation surgery. The research on spine segmentation has made great progress, but there are still some problems that prevent it from being applied in clinical simulation. Related research has been trying to solve the problems. For the ablation surgery of the spine, some researchers have tried ablation simulation and obtained simulation results that are relatively consistent with the actual temperature value.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2021.04.014DOI Listing
July 2021

COVID term: a bilingual terminology for COVID-19.

BMC Med Inform Decis Mak 2021 08 3;21(1):231. Epub 2021 Aug 3.

Institute of Medical Information/Library, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.

Background: The coronavirus disease (COVID-19), a pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has shown its destructiveness with more than one million confirmed cases and dozens of thousands of death, which is highly contagious and still spreading globally. World-wide studies have been conducted aiming to understand the COVID-19 mechanism, transmission, clinical features, etc. A cross-language terminology of COVID-19 is essential for improving knowledge sharing and scientific discovery dissemination.

Methods: We developed a bilingual terminology of COVID-19 named COVID Term with mapping Chinese and English terms. The terminology was constructed as follows: (1) Classification schema design; (2) Concept representation model building; (3) Term source selection and term extraction; (4) Hierarchical structure construction; (5) Quality control (6) Web service. We built open access for the terminology, providing search, browse, and download services.

Results: The proposed COVID Term include 10 categories: disease, anatomic site, clinical manifestation, demographic and socioeconomic characteristics, living organism, qualifiers, psychological assistance, medical equipment, instruments and materials, epidemic prevention and control, diagnosis and treatment technique respectively. In total, COVID Terms covered 464 concepts with 724 Chinese terms and 887 English terms. All terms are openly available online (COVID Term URL: http://covidterm.imicams.ac.cn ).

Conclusions: COVID Term is a bilingual terminology focused on COVID-19, the epidemic pneumonia with a high risk of infection around the world. It will provide updated bilingual terms of the disease to help health providers and medical professionals retrieve and exchange information and knowledge in multiple languages. COVID Term was released in machine-readable formats (e.g., XML and JSON), which would contribute to the information retrieval, machine translation and advanced intelligent techniques application.
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http://dx.doi.org/10.1186/s12911-021-01593-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329642PMC
August 2021

"Fishing net" suture augmenting tension-band wiring fixation in the treatment of inferior pole fracture of the patella.

Arch Orthop Trauma Surg 2021 Aug 3. Epub 2021 Aug 3.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, People's Republic of China.

Introduction: Inferior pole fracture of the patella (IPFP) is difficult to repair and stabilize clinically. Although various fixation techniques have been developed, fixation strength and mobility remain daunting challenges to orthopedic surgeons. The goal of this research is to evaluate the biomechanical strength and clinical outcomes of a novel "fishing net" suture fixation procedure.

Materials And Methods: Four finite element models, modified tension-band wiring fixation, anchor suture fixation, basket plate fixation and "fishing net" suture fixation were built to compare the fixing efficacy of "fishing net" suture fixation with three other fixation methods during IPFP fixation. From January 2018 to February 2019, 17 patients who suffered IPFP and treated by "fishing net" suture (FNS) fixation were compared with 20 patients treated by tension-band wiring (TBW) fixation in database and the two groups were evaluated postoperatively using the modified Cincinnati knee rating system.

Results: Biomechanical evaluation showed that the relative displacement values of proximal patella measured by three pairs of points on both sides of the fracture line were the lowest using the "fishing net" suture fixation, while fixation using tension-band wiring and basket plate showed similar levels of stability that were less desirable than the "fishing net" method. As to clinical outcomes, there were 17 (100%) patients exhibited excellent or good results with no internal fixation failures in the FNS group compared to three internal fixation failures in the TBW group.

Conclusion: The biomechanical and clinical results suggest that the "fishing net" suture fixation is a viable candidate for fixation of IPFP.
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http://dx.doi.org/10.1007/s00402-021-04089-3DOI Listing
August 2021

The effects of combined cognitive training on prospective memory in older adults with mild cognitive impairment.

Sci Rep 2021 Aug 2;11(1):15659. Epub 2021 Aug 2.

Department and Institute of Psychology, Ningbo University, No. 616 Fenghua Road, Jiangbei District, Ningbo, 315211, China.

This study aimed to assess the effects of combined cognitive training on prospective memory ability of older adults with mild cognitive impairment (MCI). A total of 113 participants were divided into a control group and three intervention groups. Over three months, the control group received only community education without any training, whereas for the first six weeks, an executive function training group received executive function training, a memory strategy training group received semantic encoding strategy training, and the combined cognitive training group received executive function training twice a week for the first six weeks, and semantic encoding strategy training twice a week for the next six weeks. The combined cognitive training group showed improvement on the objective neuropsychological testing (Montreal Cognitive Assessment scale). The memory strategy training group showed improvement on the self-evaluation scales (PRMQ-PM). Combined cognitive training improved the prospective memory and cognitive function of older adults with MCI.
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http://dx.doi.org/10.1038/s41598-021-95126-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329258PMC
August 2021

Victimization and depressive symptoms among Chinese adolescents: A moderated mediation model.

J Affect Disord 2021 Nov 20;294:375-381. Epub 2021 Jul 20.

School of Humanities and Social Sciences, University of Science and Technology of China, No. 96 Jinzhai Road, Baohe District, Hefei 230022, PR China. Electronic address:

Background: Victimization as an inducing factor of depressive symptoms has been confirmed in previous studies. However, little is known about how and when it induces depressive symptoms in adolescents.

Methods: In total, 1174 Chinese adolescents were recruited to complete the Juvenile Victimization Questionnaire, Security Questionnaire, Positive Psychological Capital Questionnaire, and the Chinese version of the Center for Epidemiologic Studies Depression Scale.

Results: After controlling for gender and school type, the sense of security partially mediated the association between victimization and depressive symptoms. Moreover, direct association and the mediating effect of the sense of security were moderated by psychological capital. The moderating effect occurred in the second half of the mediating effect.

Limitations: Causal conclusions cannot be drawn based on cross-sectional research design. All measures were based on participant self-report.

Conclusions: The mediating model constructed in this study emphasized the important influence of stress, emotion, and psychological diathesis on adolescent depressive symptoms.
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http://dx.doi.org/10.1016/j.jad.2021.07.022DOI Listing
November 2021

Magnetic field sensor based on helical long-period fiber grating with a three-core optical fiber.

Opt Express 2021 Jun;29(13):20649-20656

A high sensitivity optical fiber magnetic field sensor is proposed and implemented by using a helical long-period fiber grating (HLPFG) based on a three-core fiber (TCF) bonded to a U-shaped aluminum (Al) wire. An electrical current flowing through the Al wire in a perpendicular magnetic field can generate Ampere force, which changes the distance between the two arms of the U-shaped Al wire. Thus, when the intensity and direction of the magnetic field change, the bending curvature of TCF-HLPFG bonded to the U-shaped Al wire varies with the change of Ampere force, which is represented as the shift of resonant wavelength in the spectrum. The as-fabricated sensor can respond to the magnetic field direction and the intensity with a range from -15 mT to 15 mT, and the measured sensitivity is 456.5 pm/mT with Al wire electrical current 1A. The proposed sensor has the advantages of low cost, nondestructive measurement method and ease manufacture, and is expected to be applied to weak magnetic field measurements.
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http://dx.doi.org/10.1364/OE.429957DOI Listing
June 2021

Temperature-insensitive directional transverse load sensor based on dual side-hole fiber Bragg grating.

Opt Express 2021 Jun;29(12):17700-17709

We propose and demonstrate a temperature-insensitive directional transverse load sensor based on a fiber Bragg grating (FBG) inscribed in a section of dual side-hole fiber (DSHF). The application of transverse load results in an effective change in the refractive index and, consequently, changes in the DSHF birefringence. The directional transverse load response of the fabricated DSH-FBG was studied by monitoring the wavelength separations with transverse load applied in different direction with 15° increments. The load sensitivity exhibited two maxima and two minima in a polar coordinate system, achieving a maximum value of 699 pm/(N/mm) for transverse load applied along the slow axis and a minimum value of 285 pm/(N/mm) for transverse load applied along the fast axis. Subsequently, a finite element analysis (FEA) was conducted to simulate the resulting strain distribution of the DSHF with applied directional transverse load. The temperature response of the DSH-FBG transverse load sensor was also tested, yielding a low sensitivity of 1.5 × 10 pm/°C. Hence, the compact size, directional transverse load sensitivity, and temperature insensitivity of this device make it suitable for intelligent transverse load monitoring.
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http://dx.doi.org/10.1364/OE.415082DOI Listing
June 2021

Corrigendum: Interactive Brain Activity: Review and Progress on EEG-Based Hyperscanning in Social Interactions.

Front Psychol 2021 7;12:681900. Epub 2021 May 7.

School of Humanities and Social Science, University of Science and Technology of China, Hefei, China.

[This corrects the article DOI: 10.3389/fpsyg.2018.01862.].
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http://dx.doi.org/10.3389/fpsyg.2021.681900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138575PMC
May 2021

The correlation between neonatal vitamin D levels and the risk of childhood asthma attacks.

Transl Pediatr 2021 Apr;10(4):914-920

Department of Neonatology, Haikou Hospital of The Maternal And Child Health, Haikou, China.

Background: To explore the correlation between neonatal vitamin D levels and the risk of asthma in children aged 3-9 years.

Methods: A total of 1,534 subjects were selected, and clinical and laboratory test data of all subjects were collected at birth, including 25-hydroxyvitamin D3 [25(OH)D3] levels, gender, season of birth, birth weight; whether delivery was premature, cesarean section, or primipara; and parental details including maternal age, ethnicity, level of education, history of smoking, and history of maternal or paternal asthma. The level of 25(OH)D3 was used as a continuous variable to analyze its correlation with the risk of asthma using the Cox proportional regression model. In addition, subjects were divided into four groups according to their 25(OH)D3 quartiles, and the first quantile group was used as a reference to analyze the risk of asthma in the other groups. Further subgroup analysis was conducted according to the patients' gender and birth season.

Results: Among the 1,534 subjects included, there were 107 cases in the asthma-affected group and 1,427 cases in the normal control group. Compared with the control group, the level of 25(OH)D3 in the asthma group was significantly lower (P=0.03), the mother's education level was lower (P=0.002), but the preterm birth rate, cesarean section rate, and parental asthma prevalence rate were significantly higher (all P<0.05). When the neonatal 25(OH)D3 was divided into quartiles to perform a categorical variable analysis, we found that compared with the first quantile (Q1, 0.0-25.1 mmol/L), the risk of asthma in Q4 was reduced by 50% (HR=0.50, 95% CI: 0.38-0.76). The second quantile group (Q2) and the third quantile group (Q3) showed no significant difference in the risk of disease compared with Q1 but showed a trend of decreasing risk as the quantile group increased (the trend P values were both <0.05). This correlation was still valid when a stratified analysis was made based on gender and birth season.

Conclusions: There is a significant correlation between a reduced vitamin D level in newborns and the subsequent risk of asthma in children aged 3-9 years. Moreover, the level of vitamin D is an independent risk factor for childhood asthma.
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http://dx.doi.org/10.21037/tp-21-92DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107849PMC
April 2021

Broadband tunable orbital angular momentum mode converter based on a conventional single-mode all-fiber configuration.

Opt Express 2021 May;29(10):15595-15603

A broadband tunable orbital angular momentum (OAM) mode converter based on a helical long-period fiber grating (HLPFG) inscribed in a conventional single-mode fiber (SMF) is experimentally demonstrated. The proposed all-fiber OAM mode converter is based on the core-cladding mode dual resonance near the dispersion turning point (DTP). The converter can operate with a bandwidth of 303.9 nm @ -3 dB and 182.2 nm @ -10 dB, which is, as far as we know, the widest bandwidth for a conventional SMF. Furthermore, the bandwidth of the OAM mode can be dynamically tuned within a large dynamic range (>80 nm) by simply twisting the fiber clockwise (CW) or counterclockwise (CCW). The dynamic tunability of the bandwidth of the proposed OAM mode generator may find vital applications in large-capacity optical fiber communication systems.
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http://dx.doi.org/10.1364/OE.421967DOI Listing
May 2021

Fiber optic hydrogen sensor based on a Fabry-Perot interferometer with a fiber Bragg grating and a nanofilm.

Lab Chip 2021 05;21(9):1752-1758

Shenzhen Key Laboratory of Photonic Devices and Sensing Systems for Internet of Things, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China. and Guangdong and Hong Kong Joint Research Centre for Optical Fibre Sensors, Shenzhen University, Shenzhen 518060, China.

Hydrogen is widely used in industrial production and clinical medicine, and as fuel. Hydrogen becomes explosive when the hydrogen-air mixture ranges from 4 to 76 vol%; thus, a rapid hydrogen concentration measurement is particularly important in practical applications. We present a novel fiber optic hydrogen sensor with fast response fabricated from a graphene-Au-Pd sandwich nanofilm and an ultrashort fiber Bragg grating. The response time is only 4.3 s at a 3.5 vol% hydrogen concentration. When the measured hydrogen concentration was increased from 0 to 4.5 vol%, the optical resonance dip in the sensor near 1550 nm shifted by 290 pm. In addition, the sensor has an insertion loss of only -2.22 dB, a spectral contrast of 10.8 dB, and a spectral finesse of 5. Such a flexible, fast-response sensor is expected to be used in the development of hydrogen sensors with low power consumption.
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http://dx.doi.org/10.1039/d1lc00012hDOI Listing
May 2021

Identification and Distinction of Tenocytes and Tendon-Derived Stem Cells.

Front Cell Dev Biol 2021 16;9:629515. Epub 2021 Apr 16.

Department of Orthopaedics, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Restoring the normal structure and function of injured tendons is one of the biggest challenges in orthopedics and sports medicine department. The discovery of tendon-derived stem cells (TDSCs) provides a novel perspective to treat tendon injuries, which is expected to be an ideal seed cell to promote tendon repair and regeneration. Because of the lack of specific markers, the identification of tenocytes and TDSCs has not been conclusive in the study of tendons. In addition, the morphology of tendon derived cells is similar, and the comparison and identification of tenocytes and TDSCs are insufficient, which causes some obstacles to the study of tendon. In this review, the characteristics of tenocytes and TDSCs are summarized and compared based on some existing research results (mainly in terms of biomarkers), and a potential marker selection for identification is suggested. It is of profound significance to further explore the mechanism of biomarkers and to find more specific markers.
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http://dx.doi.org/10.3389/fcell.2021.629515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085586PMC
April 2021

Heterogeneous bi-directional recurrent neural network combining fusion health indicator for predictive analytics of rotating machinery.

ISA Trans 2021 Apr 22. Epub 2021 Apr 22.

State Key Laboratory for Manufacturing and Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, PR China.

Data-driven intelligent methods arise the increasing demand for predictive analytics to evaluate the operational reliability and natural degradation of rotating machinery. Nevertheless, accurate and timely predictive analytics is still regarded as an extremely challenging mission, because the quality of predictive maintenance depends not only on the capability of intelligent model, but also on the construction of effective health indicators To overcome this issue, a novel heterogeneous bi-directional gated recurrent unit (GRU) model combining with fusion health indicator (Fusion-HI) is proposed for predictive analytics in this paper. First, the support evidence space is constructed to reflect the operating state of mechanical equipment. Then the evidence features from multiple domains are integrated to obtain the optimal Fusion-HI by the modified de-noising auto-encoder (MDAE). Finally, a hybrid prediction network is designed combining with the gate attention algorithm, which consists of multi-scale convolution layers, bi-directional GRU layers, smoothed and de-noised layers, and regression layers. Three experimental whole lifetime data and one industrial entire life cycle data are analyzed to validate the feasibility of the proposed approach in two case studies respectively. Relevant experimental results indicate that the Fusion-HI is capable to sensitively characterize the degradation state of equipment, while the prediction accuracy of presented heterogeneous model is superior to that of conventional prediction approaches. .
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http://dx.doi.org/10.1016/j.isatra.2021.04.024DOI Listing
April 2021

Stretchable, self-healable integrated conductor based on mechanical reinforced graphene/polyurethane composites.

J Colloid Interface Sci 2021 Sep 8;597:393-400. Epub 2021 Apr 8.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Science and Technology of China, Hefei 230026, PR China; School of Materials Science and Engineering, Shanghai University, Shanghai 200436, PR China. Electronic address:

Stretchable conductors are susceptible to wear through repeated deformation over time. Stretchable conductors with self-healing properties can increase longevity and reduce safety hazards. However, most current self-healing conductors can only repair either the conductive layer or the insulating layer. Meantime, high mechanical robustness and self-healing efficiency are exclusive especially at ambient conditions. Realizing a stretchable conductor with integral self-healing and ultra-high mechanical strength is challenging, because this requires good interfacial compatibility and adaptability of the conductive and insulating layers. We adapt a biphasic dynamic network strategy to add toughness to self-healing materials. The DOU (dimethylglyoxime-urethane polyurethane) dynamic bonds and hydrogen bonds in the soft phase enable high self-healing efficiency, while the graphene as a hard phase supports the material's superior mechanical properties. We have prepared an overall self-healing stretchable conductor through the soft phase as a self-encapsulating insulating layer. This all-solid (Tg = -49.5 °C) graphene/dimethylglyoxime-urethane polyurethane (Gr/DOU-PU) composites characteristic of both high mechanical strength (~6 MPa, ~1000%, ~48 MJ m), self-healing conductivity (~90%, 10 min, 25 °C) and conductivity (R□=47.8 Ω □, d = 0.4 mm). The conductor has excellent stability for flexible electronics and for building stress sensors.
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http://dx.doi.org/10.1016/j.jcis.2021.04.005DOI Listing
September 2021

miR-496/MMP10 Is Involved in the Proliferation of IL-1β-Induced Fibroblast-Like Synoviocytes Via Mediating the NF-κB Signaling Pathway.

Inflammation 2021 Aug 6;44(4):1359-1369. Epub 2021 Feb 6.

Department of Rheumatology, Tianjin First Central Hospital, No.24 FuKang Road, Nankai District, Tianjin, 300192, China.

Rheumatoid arthritis (RA) is a common chronic autoimmune disease featured by synovial inflammation. miR-496 is closely involved in various pathologic conditions. However, its role in RA has not yet been elucidated. Expression of miR-496 and MMP10 was determined based on the clinical samples with RA retrieved from the Gene Expression Omnibus (GEO) datasets. In vitro model of RA was constructed in MH7A cells stimulated by IL-1β (10 ng/mL). Cell counting kit 8 (CCK-8) and flow cytometry experiments were implemented to investigate the cell viability and apoptosis rate of MH7A cells. TargetScan was applied to identify the targets of miR-496, and the regulation of miR-496 on MMP10 expression was validated by a dual-luciferase reporter gene assay. qRT-PCR and western blot analyses were conducted to examine the expression. miR-496 expression was decreased in RA tissues and MH7A cells after IL-1β treatment. Overexpression of miR-496 significantly inhibited IL-1β-treated MH7A cell viability. MMP10 was identified as a target of miR-496 and its expression was negatively regulated by miR-496. The effects of miR-496 on MH7A cell proliferation and apoptosis were reversed by MMP10. The activity of NF-κB pathway was associated with the miR-496/MMP10 axis in IL-1β-stimulated MH7A cells. To summarize, this study demonstrated that miR-496 can impair the proliferative ability and facilitate the apoptosis of IL-1β-treated MH7A through regulating MMP10 expression and NF-κB signaling pathway.
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http://dx.doi.org/10.1007/s10753-021-01421-2DOI Listing
August 2021

The potential role and trend of HIF‑1α in intervertebral disc degeneration: Friend or foe? (Review).

Mol Med Rep 2021 04 4;23(4). Epub 2021 Feb 4.

Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.

Lower back pain (LBP) is one of the most common reasons for seeking medical advice in orthopedic clinics. Increasingly, research has shown that symptomatic intervertebral disc degeneration (IDD) is mostly related to LBP. This review first outlines the research and findings of studies into IDD, from the physiological structure of the intervertebral disc (IVD) to various pathological cascades. The vicious cycles of IDD are re‑described in relation to the analysis of the relationship among the pathological mechanisms involved in IDD. Interestingly, a 'chief molecule' was found, hypoxia‑inducible factor‑1α (HIF‑1α), that may regulate all other mechanisms involved in IDD. When the vicious cycle is established, the low oxygen tension activates the expression of HIF‑1α, which subsequently enters into the hypoxia‑induced HIF pathways. The HIF pathways are dichotomized as friend and foe pathways according to the oxygen tension of the IVD microenvironment. Combined with clinical outcomes and previous research, the trend of IDD development has been predicted in this paper. Lastly, an early precautionary diagnosis and treatment method is proposed whereby nucleus pulposus tissue for biopsy can be obtained through IVD puncture guided by B‑ultrasound when the patient is showing symptoms but MRI imaging shows negative results. The assessment criteria for biopsy and the feasibility, superiority and challenges of this approach have been discussed. Overall, it is clear that HIF‑1α is an indispensable reference indicator for the accurate diagnosis and treatment of IDD.
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http://dx.doi.org/10.3892/mmr.2021.11878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893690PMC
April 2021

Necessary problems in re-emergence of COVID-19.

World J Clin Cases 2021 Jan;9(1):1-7

College of Animal Sciences, Jilin University, Changchun 130062, Jilin Province, China.

The ongoing pandemic of coronavirus disease 2019 poses a great threat to human beings. Although numerous patients have recovered, re-positive cases have been reported in several countries. Till now, we still know very little about the disease and its pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, more attention should be paid to the following aspects, such as post-discharge surveillance, asymptomatic infection, re-evaluation of influenza-like symptoms, and dynamic monitoring of genomic mutation of SARS-CoV-2.
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http://dx.doi.org/10.12998/wjcc.v9.i1.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809654PMC
January 2021

Identification of circ-FAM169A sponges miR-583 involved in the regulation of intervertebral disc degeneration.

J Orthop Translat 2021 Jan 6;26:121-131. Epub 2020 Nov 6.

Department of Orthopaedics, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.

Objective: Low back pain (LBP) is the predominant cause of disc degeneration in patients, which brings serious social problems and economic burdens. Increasing evidence has indicated that intervertebral disc degeneration (IDD) is one of the most common causes triggering LBP. Accumulating evidence has shown that circRNAs are involved in the pathological process of IDD. Nevertheless, the circRNA-mediated IDD pathogenesis still remains unknown. This study explored the potential mechanism and functions of circ-FAM169A in NPCs.

Methods: Bioinformatics analysis was conducted to identify key circRNA, miRNA and mRNA and predict their potential role in IDD. Dual-luciferase reporter assay, western blot, qRT-PCR, and fluorescence hybridisation (FISH) were used to demonstrate the interaction among circ-FAM169A, miR-583 and Sox9 in NPCs.

Results: Herein, we identified circ-FAM169A, which was dramatically up-regulated in degenerative nucleus pulposus (NP) tissues and negatively correlated with expression levels of miR-583. We constructed a circ-FAM169A-miR-583-mRNAs co-expression network and predicted circ-FAM169A-miR-583 pathway predominantly involved in extracellular matrix metabolism and cell apoptosis etc. FISH experiments confirmed circ-FAM169A and miR-583 co-existence in the cytoplasm of NPCs. Luciferase reporter assay illustrated that circ-FAM169A was directly bound to miR-583 and Sox9 was the directly target gene of miR-583. Additionally, miR-583 negatively regulated Sox9 mRNA and protein levels in NPCs.

Conclusion: Findings of this study indicated that circ-FAM169A-miR-583 pathway may play a significant role in the regulation of IDD, which will provide novel diagnostic biomarkers and develop effective treatment strategy of IDD diseases.

The Translational Potential Of This Article: This study suggested that circ-FAM169A-miR-583 pathway may regulate NPCs apoptosis and extracellular matrix synthesis and catabolism by targeting Sox9. It provides a novel therapeutic target and strategy for IVDD diseases.
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http://dx.doi.org/10.1016/j.jot.2020.07.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773979PMC
January 2021

3D nanoprinted kinoform spiral zone plates on fiber facets for high-efficiency focused vortex beam generation.

Opt Express 2020 Dec;28(25):38127-38139

In this paper, we propose and demonstrate an all-fiber high-efficiency focused vortex beam generator. The generator is fabricated by integrating a kinoform spiral zone plate (KSZP) on the top of the composite fiber structure using fs-laser two-photon polymerization 3D nanoprinting. The KSZP with spiral continuous-surface relief feature is designed by superimposing a spiral phase into a kinoform lens, which can efficiently concentrate and transform an all incident beam to a single-focus vortex beam, without the undesired zero-order diffracted light and extra high-order focus. Under arbitrary polarized light incident conditions, experiment results show that the focusing efficiency and vortex purity of the all-fiber generators are over 60% and 86%, respectively, which is much higher than that of a traditional binary SZP integrated on an optical fiber facet. In addition, characteristics of the generated vortex beam, such as focal spot, focal length and vortex topological charge are numerically designed and experimentally investigated. The experimental results agree well with the numerical simulation model using the FDTD algorithm. Due to the compact size, flexible design, polarization insensitivity, high focusing efficiency and high vortex purity, the proposed all-fiber photonic devices have promising potential in optical communication, particle manipulation and quantum computation applications.
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http://dx.doi.org/10.1364/OE.411209DOI Listing
December 2020

Promotion of collagen deposition during skin healing through Smad3/mTOR pathway by parathyroid hormone-loaded microneedle.

Mater Sci Eng C Mater Biol Appl 2021 Feb 27;119:111446. Epub 2020 Aug 27.

Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China. Electronic address:

Skin wounds are associated with huge economic and emotional burdens for millions of people annually and are a challenge for health workers worldwide. At present, for skin defects after traumatic accidents, especially large-area skin defects, newly developed strategies such as the use of emerging biomaterials and cell therapy could be considered as options besides classic skin grafts. However, the new strategies have to deal with problems such as immune rejection and high costs for patients. An insufficient understanding of the mechanisms of skin wound healing further hinders the development of innovative treatment approaches. In this study, we developed a parathyroid hormone (PTH)-loaded phase-transition microneedle (PTMN) patch to deliver PTH subcutaneously in an efficient manner and change microneedle patch daily to achieve intermittent and systematic drug administration. By evaluating wound closure, re-epithelialization, collagen deposition, and extracellular matrix (ECM) expression in a Sprague-Dawley rat model of traumatic skin wounds, we demonstrated that intermittent systemic administration of PTH using our PTMN patches accelerated skin wound healing. Further, we demonstrated that the use of the patch may accelerate skin wound healing depending on the activation of the transforming growth factor (TGF)-β/Smad3/mammalian target of rapamycin (mTOR) cascade pathway. Our results suggest that the PTH-loaded PTMN patch may be a novel therapeutic strategy for treating skin wounds.
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http://dx.doi.org/10.1016/j.msec.2020.111446DOI Listing
February 2021

Fiber Bragg Grating with Enhanced Cladding Modes Inscribed by Femtosecond Laser and a Phase Mask.

Sensors (Basel) 2020 Dec 8;20(24). Epub 2020 Dec 8.

Shenzhen Key Laboratory of Photonic Devices and Sensing Systems for Internet of Things, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

In this paper, we demonstrate a fiber Bragg grating (FBG) with a wide range and a comb with continuous cladding mode resonances inscribed in non-photosensitive single mode fibers using a femtosecond laser and a phase mask. The FBG is inscribed in the core and cladding, exciting a series of cladding modes in transmission. The birefringence induced by this FBG structure offers significant polarization-dependence for cladding modes, thus allowing the vector fiber twist to be perceived. By measuring the peak-to-peak differential intensity of orthogonally polarized cladding mode resonances, the proposed sensor presents totally opposite intensity response in the anticlockwise direction for the torsion angle ranging from -45° to 45°. The cladding mode comb approximately covers wavelengths over the O-, E-, S-, and C-bands in transmission. The cutoff cladding mode of air can be observed in the spectrum. Thus, the sensible refractive index range is estimated to be from 1.00 to 1.44. Temperature responsivity of the grating is also characterized. The proposed device potentially provides new solutions to the various challenges of physical vector and bio-chemical parameters sensing.
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http://dx.doi.org/10.3390/s20247004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762558PMC
December 2020

Identification of adhesion-associated DNA methylation patterns in the peripheral nervous system.

Exp Ther Med 2021 Jan 18;21(1):48. Epub 2020 Nov 18.

Department of Orthopedics, Tianjin Medical University General Hospital, Heping, Tianjin 300052, P.R. China.

Schwann cells are unique glial cells in the peripheral nervous system. These cells provide a range of cytokines and nutritional factors to maintain axons and support axonal regeneration. However, little is known concerning adhesion-associated epigenetic changes that occur in Schwann cells after peripheral nerve injury (PNI). In the present study, adhesion-associated DNA methylation biomarkers were assessed between normal and injury peripheral nerve. Specifically, normal Schwann cells (NSCs) and activated Schwann cells (ASCs) were obtained from adult Wistar rats. After the Schwann cells were identified, proliferation and adhesion assays were used to assess differences between NSCs and ASCs. Methylated DNA immunoprecipitation-sequencing and bioinformatics analysis were used to identify and analyze the differentially methylated genes. Reverse transcription-quantitative PCR was performed to assess the expression levels of adhesion-associated genes. In the present study, the proliferation and adhesion assays demonstrated that ASCs had a more robust proliferative activity and adhesion compared with NSCs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed to identify methylation-associated biological processes and signaling pathways. Protein-protein interaction network analysis revealed that Fyn, Efna1, Jak2, Vav3, Flt4, Epha7, Crk, Kitlg, Ctnnb1 and Ptpn11 were potential markers for Schwann cell adhesion. The expression levels of several adhesion-associated genes, such as vinculin, BCAR1 scaffold protein, collagen type XVIII α1 chain and integrin subunit β6, in ASCs were altered compared with those in NSCs. The current study analyzed adhesion-associated DNA methylation patterns of Schwann cells and identified candidate genes that may potentially regulate Schwann cell adhesion in Wistar rats before and after PNI.
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http://dx.doi.org/10.3892/etm.2020.9479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706384PMC
January 2021

[Surgical efficacy analysis of venous pulsatile tinnitus related to sigmoid sinus diverticulum].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2020 Dec;34(12):1079-1083

Department of Otolaryngology Head and Neck Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing,100050,China.

To discuss the outcomes of the surgical treatment of venous pulsatile tinnitus caused by sigmoid sinus diverticulum. Fifty-eight patients with venous pulsatile tinnitus caused by unilateral sigmoid sinus diverticulum were admitted. The patients' tinnitus was graded and treated by sigmoid sinus bone wall reconstruction. The maximum cross-sectional area of sigmoid sinus diverticulum defect was measured in 28 patientsc. All the patients were followed up for 3 to 44 months after operation to analyze the improvement of postoperative tinnitus, the relationship between the size of the sigmoid sinus cross-sectional area and the degree of tinnitus and length of disease statistically. Postoperative tinnitus was cured in 41 cases(70.69%),markedly effective in 10 cases(17.24%), effective in 3 cases(5.17%) and ineffective in 4 cases(6.90%). There was a statistically significant difference in the degree of tinnitus before and after surgery (<0.05). Pearson correlation analysis showed thatthe maximum cross-sectional area of the sigmoid sinus diverticulum defect was not significantly correlated with the degree of tinnitus and the length of the disease in 28 patients (>0.05). The sigmoid sinus bone wall reconstruction can effectively relieve venous pulsatile tinnitus related to sigmoid sinus diverticulum while ensuring patient safety.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2020.12.006DOI Listing
December 2020

Toxicity of Cry1-Class, Cry2Aa, and Vip3Aa19 Bt proteins and their interactions against yellow peach Moth, Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae).

J Invertebr Pathol 2021 01 26;178:107507. Epub 2020 Nov 26.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuanmingyuan Road, Beijing 100193, China. Electronic address:

Transgenic plants expressing insecticidal proteins from the Bacillus thuringiensis (Bt) have provided an effective way to control target pests. However, the toxicity of Bt proteins against yellow peach moth (YPM), Conogethes punctiferalis (Guenée), one of the most serious maize pests in China, has not received much study. Therefore, we performed diet-overlay bioassays to evaluate the insecticidal activities of Cry1Ab, Cry1Ac, Cry1Fa, Cry1Ah, Cry1Ie, Cry2Aa, and Vip3Aa19, as well as the interaction between Cry1-Class, Cry2Aa, and Vip3Aa19 against YPM. Results showed that the LC values ranged from 1.08 to 178.12 ng/cm (protein/diet). Among these proteins, Cry1Ab and Cry1Ac had lower LC values and LC values. In YPM bioassays, the combinations of Cry2Aa with Cry1Ac, Cry1Ie, and Cry1Ab showed antagonism while a mixture of Cry2Aa with Cry1Fa and Cry1Ah exhibited synergism. When Vip3Aa19 was combined with Cry proteins, all combinations interacted positively, with variation in synergistic factors (SF). Three ratios 1:1, 1:2, and 2:1 of Cry1Ah and Vip3Aa19 protein combination showed SF values of 5.20, 5.63, and 8.98, respectively. These findings can be applied in the establishment of new pyramided transgenic crops with suitable candidates as well as in resistance management strategies.
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http://dx.doi.org/10.1016/j.jip.2020.107507DOI Listing
January 2021

Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.

Med Sci Monit 2020 Nov 4;26:e924266. Epub 2020 Nov 4.

Department of Orthopaedics, Tianjin Medical University General Hospital, International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Key Laboratory of Spine and Spinal Cord, Tianjin, China (mainland).

BACKGROUND Spinal cord injury (SCI) is a devastating trauma of the central nervous system (CNS), with high levels of morbidity, disability, and mortality. One week after SCI may be a critical time for treatment. Changes in protein expression have crucial functions in nervous system diseases, although the effects of changes occurring 1 week after SCI on patient outcomes are unclear. MATERIAL AND METHODS Protein expression was examined in a rat contusive SCI model 1 week after SCI. Differentially expressed proteins (DEPs) were identified by isobaric tagging for relative and absolute protein quantification (iTRAQ)-coupled liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomics analysis. Gene Ontology (GO) analysis was performed to identify the biological processes, molecular functions, and cellular component terms of the identified DEPs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to identify key enriched pathways. Protein-protein interaction (PPI) networks were analyzed to identify the top 10 high-degree core proteins. RESULTS Of the 295 DEPs identified, 204 (69.15%) were upregulated and 91 (30.85%) were downregulated 1 week after injury. The main cellular components, molecular functions, biological processes, and pathways identified may be crucial mechanisms involved in SCI. The top 10 high-degree core proteins were complement component C3 (C3), alpha-2-HS-glycoprotein (Ahsg), T-kininogen 1 (Kng1), Serpinc1 protein (Serpinc1), apolipoprotein A-I (Apoa1), serum albumin (Alb), disulfide-isomerase protein (P4hb), transport protein Sec61 subunit alpha isoform 1 (Sec61a1), serotransferrin (Tf), and 60S ribosomal protein L15 (Rpl15). CONCLUSIONS The proteins identified in this study may provide potential targets for diagnosis and treatment 1 week after SCI.
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http://dx.doi.org/10.12659/MSM.924266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650090PMC
November 2020

Building a Pharmacogenomics Knowledge Model Toward Precision Medicine: Case Study in Melanoma.

JMIR Med Inform 2020 Oct 21;8(10):e20291. Epub 2020 Oct 21.

Institute of Medical Information &Library, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.

Background: Many drugs do not work the same way for everyone owing to distinctions in their genes. Pharmacogenomics (PGx) aims to understand how genetic variants influence drug efficacy and toxicity. It is often considered one of the most actionable areas of the personalized medicine paradigm. However, little prior work has included in-depth explorations and descriptions of drug usage, dosage adjustment, and so on.

Objective: We present a pharmacogenomics knowledge model to discover the hidden relationships between PGx entities such as drugs, genes, and diseases, especially details in precise medication.

Methods: PGx open data such as DrugBank and RxNorm were integrated in this study, as well as drug labels published by the US Food and Drug Administration. We annotated 190 drug labels manually for entities and relationships. Based on the annotation results, we trained 3 different natural language processing models to complete entity recognition. Finally, the pharmacogenomics knowledge model was described in detail.

Results: In entity recognition tasks, the Bidirectional Encoder Representations from Transformers-conditional random field model achieved better performance with micro-F1 score of 85.12%. The pharmacogenomics knowledge model in our study included 5 semantic types: drug, gene, disease, precise medication (population, daily dose, dose form, frequency, etc), and adverse reaction. Meanwhile, 26 semantic relationships were defined in detail. Taking melanoma caused by a BRAF gene mutation into consideration, the pharmacogenomics knowledge model covered 7 related drugs and 4846 triples were established in this case. All the corpora, relationship definitions, and triples were made publically available.

Conclusions: We highlighted the pharmacogenomics knowledge model as a scalable framework for clinicians and clinical pharmacists to adjust drug dosage according to patient-specific genetic variation, and for pharmaceutical researchers to develop new drugs. In the future, a series of other antitumor drugs and automatic relation extractions will be taken into consideration to further enhance our framework with more PGx linked data.
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http://dx.doi.org/10.2196/20291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641779PMC
October 2020

The relationship between health-promoting lifestyles and depression in the elderly: roles of aging perceptions and social support.

Qual Life Res 2021 Mar 17;30(3):721-728. Epub 2020 Oct 17.

Department and Institute of Psychology, Ningbo University, No. 818 Fenghua Road, Jiangbei District, Ningbo, 315211, China.

Purpose: At present, it is not clear about the influence of health-promoting lifestyle, aging perceptions, social support, and other psychosocial factors on elderly depression. This study aims to explore the mediating role of aging perceptions between health-promoting lifestyle and elderly depression, and the moderating role of social support in the mediating process.

Methods: A cross-sectional survey was conducted among 359 elderly people in six districts of a city. The Chinese version of the Health-Promoting Lifestyle Profile-II (HPLP-IIR), the Brief Aging Perceptions Questionnaire (B-PQ), the Center for Epidemiological Studies Depression Scale (CES-DR), and the Social Support Rate Scale (SSRS) were conducted and recollected on the spot. Stepwise analysis was used to test the mediating effect and moderating effect, and age and gender variables were controlled.

Results: The results showed the following: (1) health-promoting lifestyle is an important influencing factor of elderly depression; (2) aging perceptions plays a mediating role in the relationship between health-promoting lifestyle and elderly depression, accounting for 31.8% of the total utility; and (3) social support plays a moderating role between aging perceptions and elderly depression, with a high level of social support. The effect of aging perceptions on depression is less than that of the elderly with low social support level.

Conclusion: Health-promoting lifestyle influence the depression of elderly people through aging perceptions and social support moderates the influence of aging perceptions on the elderly depression.
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http://dx.doi.org/10.1007/s11136-020-02674-4DOI Listing
March 2021

PMO: A knowledge representation model towards precision medicine.

Math Biosci Eng 2020 06;17(4):4098-4114

Institute of Medical Information/Library, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100020, China.

With the rapid development of biomedical technology, amounts of data in the field of precision medicine (PM) are growing exponentially. Valuable knowledge is included in scattered data in which meaningful biomedical entities and their semantic relationships are buried. Therefore, it is necessary to develop a knowledge representation model like ontology to formally represent the relationships among diseases, phenotypes, genes, mutations, drugs, etc. and achieve effective integration of heterogeneous data. On basis of existing work, our study focus on solving the following issues: (i) Selecting the primary entities in PM domain; (ii) collecting and integrating biomedical vocabularies related to the above entities; (iii) defining and normalizing semantic relationships among these entities. We proposed a semi-automated method which improved the original Ontology Development 101 method to build the Precision Medicine Ontology (PMO), including defining the scope of the PMO according to the definition of PM, collecting terms from different biomedical resources, integrating and normalizing the terms by a combination of machine and manual work, defining the annotation properties, reusing existing ontologies and taxonomies, defining semantic relationships, evaluating PMO and creating the PMO website. Finally, the Precision Medicine Vocabulary (PMV) contains 4.53 million terms collected from 62 biomedical vocabularies, and the PMO includes eleven branches of PM concepts such as disease, chemical and drug, phenotype, gene, mutation, gene product and cell, described by 93 semantic relationships among them. PMO is an open, extensible ontology of PM, all of the terms and relationships in which could be obtained from the PMO website (http://www.phoc.org.cn/pmo/). Compared to existing project, our work has brought a broader and deeper coverage of mutation, gene and gene product, which enriches the semantic type and vocabulary in PM domain and benefits all users in terms of medical literature annotation, text mining and knowledge base construction.
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http://dx.doi.org/10.3934/mbe.2020227DOI Listing
June 2020
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