Publications by authors named "Shekoufeh Khajouei"

1 Publications

  • Page 1 of 1

Protective effect of bioactive compounds from against cisplatin-induced oxidative stress and apoptosis in the PC12 cell line.

Iran J Basic Med Sci 2017 Apr;20(4):438-445

Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Objectives: The present study aims to evaluate the protective effect of the compounds isolated from () against oxidative stress and apoptosis induced by cisplatin (CIS) in PC12 cells.

Materials And Methods: Six compounds were isolated as quercetrin-3---D-glucopyranoside (QUE), osthol (OST), verbenone-5---D-glycopyranoside (VER), Isoimperatorin (ISO), kaempferol-3---D-glucopyranoside (KAM), and echinophorin B (ECH). For this study, we used MTT reduction assay for detection of protective effects of isolated compounds on CIS-induced cytotoxicity in PC12 cells. The effects of isolated compounds against apoptosis induced by CIS were investigated through the measurement of mitochondrial membrane potential (MMP), Bax and Bcl2 mRNA expression, and caspase-3 activation. We also assessed oxidative stress by measuring reactive oxygen species (ROS) generation with 2', 7'-dichlorofluorescein diacetate (DCFH-DA).

Results: Treatment of cells with QUE and OST before exposure to the CIS increased cell viability, i.e., these compounds protected the cells against CIS -induced cytotoxicity. In addition, pre-treatment with QUE and OST decreased CIS-induced apoptosis through up-regulation of Bcl-2, inhibition of caspase-3 activity, and mitochondrial membrane potential (MMP) increase. OST decreased ROS generation induced by CIS, as well.

Conclusion: Our experiment showed that QUE and OST are apoptotic inhibitors that effectively block CIS-induced neurotoxicity predicting their therapeutic potential in the prevention of chemotherapy-induced neurotoxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.22038/IJBMS.2017.8587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425927PMC
April 2017
-->