Publications by authors named "Sheila Shaigany"

8 Publications

  • Page 1 of 1

Lack of distinct molecular profile of Primary Dermal Melanoma.

Hum Pathol 2020 Dec 15;106:32-38. Epub 2020 Sep 15.

Department of Dermatology, Dermatopathology, Columbia University Medical Center, New York, NY, USA; Department of Pathology and Laboratory Medicine, Vancouver General Hospital, University of British Columbia, Vancouver, BC, USA. Electronic address:

Primary dermal melanoma (PDM) is a rare variant of melanoma which simulates metastatic melanoma to the skin. Diagnosis of PDM cannot be established on histologic grounds alone but requires absence of evidence of melanoma elsewhere by clinical history and/or imaging studies. Despite this entity being clinically well documented, limited data on molecular characterization are available. We performed comprehensive mutation and copy number variation analysis in a series of PDMs in search for distinctive molecular features.Studies were approved by respective institutional review boards. Six cases fulfilling strict histologic criteria of PDM were identified in patients with absent history of melanoma elsewhere, negative sentinel lymph node biopsies and imaging studies. DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tissue, and five cases passed quality control measures and were subjected to targeted exon sequencing using the Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets 410 panel. Two of five PDM carried NRAS hotspot mutations characteristic of cutaneous melanoma (CM) genomic subtypes. One case showed a probable low-activating NRAS mutation in combination with additional aberrations in other mitogen-activated protein kinase (MAPK) pathway effectors. Hotspot mutations were also identified in the TERT promoter region, and one tumor showed a mutation in the SWI/SNF remodeling complex. No oncogenic mutations were identified in one case. Furthermore, none of the tumors analyzed showed activating mutations in Gα subunits, including GNAQ and GNA11. Copy number alterations included deletions of Chr 9p, characteristic of CM.Despite PDM showing mutational heterogeneity, our findings suggest predominance of MAPK pathway aberrations in agreement with the mutational profile of CMs in general. Given the absence of genetic overlap with other distinct primary dermal melanocytic proliferations, mutational profiling will unlikely aid in the difficult differential diagnosis of PDM versus melanoma metastasis. Thorough metastatic workup remains crucial in establishing this rare diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.humpath.2020.09.002DOI Listing
December 2020

An adult with Kawasaki-like multisystem inflammatory syndrome associated with COVID-19.

Lancet 2020 07 10;396(10246):e8-e10. Epub 2020 Jul 10.

Ronald O Perelman Department of Dermatology, New York University Grossman School of Medicine, New York, NY 10016, USA. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/S0140-6736(20)31526-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351414PMC
July 2020

Comparison between organismal staining on histology and tissue culture in the diagnosis of cutaneous infection: A retrospective study.

J Am Acad Dermatol 2020 Jun 28;82(6):1400-1408. Epub 2020 Jan 28.

Ronald O. Perelman Department of Dermatology, New York University School of Medicine, New York, New York. Electronic address:

Background: In instances of suspected cutaneous infection, the standard of care includes obtaining skin biopsy specimens for histology and tissue culture. Few studies have compared the clinical utility of each test.

Objective: To assess the concordance of results between tissue culture and histology, as well as the clinicopathologic features that may influence the diagnostic yield of each test.

Methods: A retrospective review of all patients who underwent skin biopsy for histology and tissue culture at New York University from 2013 through 2018.

Results: Of 179 patients, 10% had positive concordance, 21% had positive tissue culture only, and 7% had positive histology only. We calculated a kappa correlation coefficient of 0.25 between histology and tissue culture (reference, 0.21-0.39 indicates minimal agreement). Histology exhibited higher sensitivity in detecting fungi, whereas tissue culture was more sensitive in identifying Gram-negative bacteria. Antimicrobial use before biopsy led to significantly fewer positive cultures (37.5% vs 71%; P = .023) in patients ultimately diagnosed with infection.

Limitations: This study was conducted at a single institution, thereby restricting its broad applicability. The lack of a validated criterion standard to diagnose infection also limits interpretation of the results.

Conclusion: Tissue culture and histopathology often yield discordant results. Dermatologists should recognize specific limitations, yet high clinical utility in special circumstances, of tests when approaching cases of suspected infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaad.2020.01.047DOI Listing
June 2020

Enlarging red papulonodule on the chest.

Cutis 2018 Feb;101(2):78;117;118

Department of Dermatology, Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
February 2018

A case of lymphomatoid granulomatosis presenting with cutaneous lesions.

JAAD Case Rep 2015 Jul 2;1(4):234-7. Epub 2015 Jul 2.

Department of Dermatology and Dermatology Consultation Service, Columbia University Medical Center, New York, New York.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdcr.2015.05.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808726PMC
July 2015

Resident Rounds Part III: Case Report: Fatal Cryptococcal Panniculitis in a Lung Transplant Recipient.

J Drugs Dermatol 2015 May;14(5):519-22

Cryptococcal panniculitis is a rare entity previously reported in only 13 solid organ transplant (SOT) recipients. Cutaneous cryptococcosis in SOT recipients warrants extensive systemic workup and treatment as if central nervous system (CNS) disease is present. It should be included in the differential diagnosis of panniculitis in the immunocompromised host, as early diagnosis and treatment are critical. We report a fatal case of cryptococcal panniculitis in a 44-year-old lung transplant recipient.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2015

Herpetic zoster folliculitis in the immunocompromised host.

JAAD Case Rep 2015 Jan 12;1(1):38-40. Epub 2015 Jan 12.

Department of Dermatology and Dermatology Consultation Service, Columbia University Medical Center, New York, New York.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdcr.2014.11.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802560PMC
January 2015

Resolution of urticarial vasculitis after treatment of neurocysticercosis.

J Am Acad Dermatol 2015 Jan;72(1):e32-3

Department of Dermatology and Dermatology Consultation Service, Columbia University Medical Center, New York, New York.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaad.2014.09.017DOI Listing
January 2015