Sharmeen Fayyaz - Dr. Panjwani Center for Molecular Medicine and Drug Research

Sharmeen Fayyaz

Dr. Panjwani Center for Molecular Medicine and Drug Research

ORCID logohttps://orcid.org/0000-0002-2386-6793

Sharmeen Fayyaz - Dr. Panjwani Center for Molecular Medicine and Drug Research

Sharmeen Fayyaz

Introduction

Primary Affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research

Publications

6Publications

38Reads

12Profile Views

Megestrol acetate induced proliferation and differentiation of osteoblastic MC3T3-E1 cells: A drug repurposing approach.

Steroids 2020 May 19;157:108607. Epub 2020 Feb 19.

Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.steroids.2020.108607DOI Listing
May 2020
2.639 Impact Factor

Effect of Steroidal Hormone Pregnenolone on Proliferation and Differentiation of MC3T3-E1 Osteoblast like Cells

Letters in Drug Design & Discovery

Background: Bone remodeling is a complex process which includes continuous resorption by osteoclast cells and bone formation by osteoblast cells. Bone fragility is a common issue of elderly population particularly in postmenopausal women. It has been established that steroidal hormones have important role in bone homeostasis. Therefore hormone replacement therapy could have beneficial effects on bone health as compared to other treatments.

Objectives: Imbalance between the rate of bone formation and bone resorption leads to fragility of bones. During the current study, we aimed to explore the ability of pregnenolone (1) (PRE), on proliferation and differentiation of MC3T3-E1 cells. We further aimed to investigate the underlying mechanism of action for anabolic effect of PRE (1).

Methods: Effects of pregnenolone (1) on proliferation, differentiation, and mineralization of MC3T3 osteoblast like cells were determined .Cell viability was analyzed using MTT assay, and flow cytometry. ALP activity and alizarin staining were employed to evaluate the effect of pregnenolone on osteoblast differentiation. Moreover, western blot for analysis of certain important proteins, crucial for regulation of bone homeostasis, such as BMP2 and RANKL, was also performed.

Results: Our results showed that pregnenolone (1) at a concentration of 5 μM caused a significant (p< 0.05) rise in the growth of MC3T3-E1 cells, whereas a comparable effect was observed in osteoblast differentiating assays. Significant decrease in RANKL expression was observed at (0.04 – 1 µM). Our results therefore indicated the possible role of pregnenolone (1) in positive regulation of bone homeostasis by suppressing RANKL expression.

Conclusion: Taken together, our results indicate that pregnenolone (1) has the potential to enhance osteoblast proliferation, as inferred from the increased number of cells. These results demonstrated that pregnenolone (1) could be a potential anabolic agent for the treatment of fragility related disorders.

View Article
March 2020
22 Reads

Small Molecular Leads Differentially Active Against HER2 Positive and Triple Negative Breast Cancer Cell Lines.

Med Chem 2019 ;15(7):738-742

Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1573406414666181106143912DOI Listing
December 2019
23 Reads
1.387 Impact Factor

Synthesis of novel bisindolylmethanes: New carbonic anhydrase II inhibitors, docking, and 3D pharmacophore studies.

Bioorg Chem 2016 10 25;68:90-104. Epub 2016 Jul 25.

H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2016.07.011DOI Listing
October 2016
6 Reads
2.152 Impact Factor

Synthesis, biological evaluation, and docking studies of novel thiourea derivatives of bisindolylmethane as carbonic anhydrase II inhibitor.

Bioorg Chem 2015 Oct 5;62:83-93. Epub 2015 Aug 5.

Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2015.08.001DOI Listing
October 2015
9 Reads
2.152 Impact Factor

Top co-authors

Muhammad Iqbal Choudhary
Muhammad Iqbal Choudhary

H.E.J. Research Institute of Chemistry

3
Syahrul Imran
Syahrul Imran

Universiti Teknologi MARA

2
Nor Hadiani Ismail
Nor Hadiani Ismail

Atta-ur-Rahman Institute for Natural Product Discovery

2
Muhammad Taha
Muhammad Taha

Atta-ur-Rahman Institute for Natural Product Discovery

2
Khalid Mohammed Khan
Khalid Mohammed Khan

University of Karachi

2
 Atia-Tul-Wahab
Atia-Tul-Wahab

University of Karachi

2
Elias Baydoun
Elias Baydoun

American University of Beirut

1
Rong Yu
Rong Yu

Sichuan University

1