Publications by authors named "Shaomin Shuang"

181 Publications

A butterfly-shaped ESIPT molecule with solid-state fluorescence for the detection of latent fingerprints and exogenous and endogenous ONOO by caging of the phenol donor.

Talanta 2021 Oct 9;233:122593. Epub 2021 Jun 9.

Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, PR China. Electronic address:

The latent fingerprints (LFPs) at the crime scene are unique and stable, which are considered as an important clue in criminal justice and forensic identification. Herein, a butterfly-shaped molecule DPTS with solid fluorescence plus excited-state intramolecular proton transfer (ESIPT) properties was used to develop for enhancing the visualization of the LFPs. Considering the solid fluorescence of DPTS, the color and efficiency of DPTS with a large Stokes shift (216 nm) can be tuned by changing the morphology of its aggregates, and gradually red-shifted (green-yellow-red) with increasing water content. Furthermore, its effectiveness for the detection of LFPs was demonstrated on various different substrates including paper box, tinfoil and weighting paper. The emissive fingerprint of DPTS obtained gave good fluorescence images with high contrast and resolution such as the core, delta, bifurcation, ridge termination, independent ridge and pores. Caging of the phenol donor of DPTS with a sensitive biomarker group provided DPTS-ONOO, which had high sensitive with detection limit of 5 nM and the quantification limit of 21 nM toward ONOO. Modularly derived DPTS-ONOO was synthesized and demonstrated specific fluorescence imaging of exogenous and endogenous peroxynitrite (ONOO) in living macrophage cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2021.122593DOI Listing
October 2021

A fluorometric and colorimetric dual-readout nanoprobe based on Cl and N co-doped carbon quantum dots with large stokes shift for sequential detection of morin and zinc ion.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 27;261:120028. Epub 2021 May 27.

Institute of Environmental Science, and Shanxi Laboratory for Yellow River, Shanxi University, Taiyuan, Shanxi 030006, PR China. Electronic address:

A novel visual nanoprobe was developed for the sequential detection of morin and zinc ion (Zn) based on Cl and N co-doped carbon quantum dots (ClNCQDs) via a fluorometric and colorimetric dual-readout mode. The yellow fluorescence ClNCQDs was synthesized by the one-step hydrothermal treatment of o-chlorobenzoic acid and p-phenylenediamine. The most distinctive property of the ClNCQDs is the large stokes shift (177 nm), which is significantly higher than other reported CQDs. The fluorescence of the ClNCQDs can be effectively quenched by morin based on the synergistic effect of IFE, electrostatic interaction, and dynamic quenching process, and recovered upon the addition of Zn due to strong interaction between morin and Zn. The nanoprobe exhibited favorable selectivity and sensitivity toward morin and Zn with detection limits of 0.09 µM and 0.17 µM, respectively. Simultaneously, the color of the ClNCQDs solution was changed (light-pink → faint-yellow → dark-yellow) along with the variation of the fluorescence signal of the ClNCQDs. This proposed nanoprobe was successfully applied for morin and Zn analyses in actual samples and live cells with high accuracy. The results of this study demonstrate the great application prospects of the ClNCQDs for morin and Zn detection in complex actual samples and biosystems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.120028DOI Listing
November 2021

AIE-based fluorescent boronate probe and its application in peroxynitrite imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 4;261:120044. Epub 2021 Jun 4.

Institute of Environmental Science, Shanxi University, Taiyuan 030006, PR China. Electronic address:

Fluorescent probes have contributed greatly to our understanding of the biological role of peroxynitrite (ONOO). The ONOO fluorescence probe characterized by the arlyboronate received a moderate opening fluorescence response, and the borate-masked probe significantly increased the sensitivity of ONOO. Thus, two simple fluorescent probes (ADB and ANB) with the recognition receptor of phenyl boronate moiety were constructed for the detection of ONOO. The change of emission spectrum was affected differently by the electron donating (or withdrawing) of the substituents. ANB was shown to have a low sensitivity and quantum yield towards ONOO in aqueous solution, whereas ADB with aggregation-induced emission (AIE) process exhibited not only good sensitivity for ONOO with a detection limit of 75 nM, but also ADB could be used to quantitative detecting ONOO in response to concentrations of ONOO within 20 s. Importantly, ADB had good performance for the detection of exogenous ONOO in the RAW 264.7 cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.120044DOI Listing
November 2021

Ratiometric sensing of Zn with a new benzothiazole-based fluorescent sensor and living cell imaging.

Analyst 2021 Jul 11;146(13):4348-4356. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

A new fluorescent probe, 3-(benzo[d]thiazol-2-yl)-5-bromosalicylaldehyde-N-phenyl thiosemicarbazone (BTT), for ratiometric sensing of Zn ions in methanol/HEPES buffer solution (3 : 2, pH = 7.4) is reported in this paper. The presence of Zn ions yields a significant blue shift in the maximum emission of BTT from 570 nm to 488 nm, accompanied by a clear color change from orange to green. This emission change of BTT upon binding to Zn in a 1 : 1 ratio may be due to the block of excited state intramolecular proton transfer (ESIPT) as well as chelation enhanced fluorescence (CHEF) on complex formation. The limit of detection (LOD) determined for Zn quantitation was down to 37.7 nM. In addition, the probe BTT displays the ability to image both exogenous Zn ions loaded into HeLa cells and endogenous Zn distribution in living SH-SY5Y neuroblastoma cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1an00749aDOI Listing
July 2021

MnO nanosheets anchored with polypyrrole nanoparticles as a multifunctional platform for combined photothermal/photodynamic therapy of tumors.

Food Funct 2021 Jul 8;12(14):6334-6347. Epub 2021 Jun 8.

College of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.

Herein, [email protected] nanocomposites were first harvested by anchoring MnO nanosheets on polypyrrole (PPy) nanoparticles via an in situ redox reaction, then polyethylene glycol (PEG) modifier and methylene blue (MB) photosensitizer were linked through electrostatic interactions to obtain [email protected] nanoarchitectures. PPy nanoparticles ensure photothermal therapy (PTT) ability and MnO nanosheets ameliorate tumor hypoxia for enhanced photodynamic therapy (PDT). Therefore, a multifunctional nanotherapeutic system was constructed for the combined PTT/PDT of tumors. For extracellular photothermal properties, the optimal temperature elevation was 52.6 °C with 54.4% photothermal conversion efficiency. The extracellular PDT ability was measured by detecting O generation; more O was produced under acidic conditions in the presence of HO (a simulated tumor microenvironment). The effective cellular uptake of the nanotherapeutic system in HeLa cells was observed by confocal laser scanning microscopy (CLSM). CLSM also indicated that more O was generated by the nanotherapeutic system as compared to free MB in HeLa cells, confirming the amelioration of tumor hypoxia by MnO nanosheets. MTT assays demonstrated that the nanotherapeutic system possessed superior biocompatibility without laser irradiation, and the lowest cell viabilities for single PTT and PDT groups were 13.78%, 38.82% respectively, while there was only 1.29% cell viability in the combined PTT and PDT group. These results suggest that the strategy of assembling PPy with MnO for a multifunctional PTT and enhanced PDT nanoplatform was realized, and opens up an unimpeded approach for integrating photothermal reduction materials with MnO for use in synergistic PTT and PDT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo00032bDOI Listing
July 2021

Lipid Droplet-Specific Fluorescent Probe for Visualization of Polarity in Fatty Liver, Inflammation, and Cancer Models.

Anal Chem 2021 06 26;93(22):8019-8026. Epub 2021 May 26.

Institute of Environmental Science, Shanxi Laboratory for Yellow River, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, P. R. China.

Elucidating the intrinsic relationship between diseases and lipid droplet (LD) polarity remains a great challenge owing to the lack of the research on multiple disease models. Until now, the visualization of abnormal LD polarity in models of inflammation and clinical cancer patient samples has not been achieved. To meet the urgent challenge, we facilely synthesized a robust LD-specific and polarity-sensitive fluorescent probe (-), which consists of a triphenylamine segment as an electron-donor group (D) and a pyridinium as an electron-acceptor moiety (A), forming a typical D-π-A molecular configuration. Owing to the unique intramolecular charge transfer effect, exhibits high sensitivity to polarity change in the linear range from Δ = 0.258 to 0.312, with over 278-fold fluorescence enhancement. Moreover, we revealed that possessed satisfactory ability for sensitively monitoring LD-polarity changes in living cells. Using , we first demonstrated the detection of LD-polarity changes in fatty liver tissues and inflammatory living mice via confocal laser scanning fluorescence imaging. Surprisingly, the visualization of LD polarity has been achieved not only at the cellular levels and living organs but also in surgical specimens from cancer patients, thus holding great potential in the clinical diagnosis of human cancer. All these features render an effective tool for medical diagnosis of LD polarity-related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c01125DOI Listing
June 2021

Nitrogen-doped carbon dots for wash-free imaging of nucleolus orientation.

Mikrochim Acta 2021 May 10;188(6):183. Epub 2021 May 10.

Institute of Environmental Science, and Shanxi Laboratory for Yellow River, Shanxi University, Taiyuan, 030006, People's Republic of China.

Carbon dots (CDs) are a rising star in the field of cellular imaging, especially cytoplasmic imaging, attributing to the super-stable optical performance and ultra-low biological toxicity. Nucleolus can accurately reflect the expression state of a cell and is strongly linked to the occurrence and development of many diseases, so exploring bran-new CDs for nucleolus-orientation imaging with no-wash technology has important theoretical value and practical significance. Herein, nitrogen-doped carbon dots (N-CDs) with green fluorescence (the relative fluorescence quantum yield of 24.4%) was fabricated by the hydrothermal treatment of m-phenylenediamine and p-aminobenzoic acid. The N-CDs possess small size, bright green fluorescence, abundant surface functional groups, excellent fluorescence stability and good biocompatibility, facilitating that the N-CDs are an excellent imaging reagent for cellular imaging. N-CDs can particularly bind to RNA in nucleoli to enhance their fluorescence, which ensures that the N-CDs can be used in nucleolus-orientation imaging with high specificity and wash-free technique. This study demonstrates that the N-CDs have a significant feasibility to be used for nucleolus-orientation imaging in biomedical analysis and clinical diagnostic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-021-04837-7DOI Listing
May 2021

Recent advances in synthesis and applications of room temperature phosphorescence carbon dots.

Talanta 2021 Aug 31;231:122350. Epub 2021 Mar 31.

School of Chemistry and Chemical Engineering, and Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China. Electronic address:

Recently, room temperature phosphorescence (RTP) feature of carbon dots (CDs) has gradually diverted researchers' attention from fluorescence and sparks new research boom due to its ultra-long luminescence lifetime and large Stokes shift. Some attempts have been made to construct CDs-based RTP materials, and had seen some important progress. However, few review articles were published to systematically summarize them. Herein, we summarize the recent synthesis advances of the RTP CDs, mainly focusing on matrix-assisted method and self-protection method. Different construction methodologies lead to different RTP properties and luminescence mechanisms. Based on this fact, we discuss the correlation between them and further summarize their potential applications in sensing, light-emitting diodes, anti-counterfeiting, and information protection filed. Finally, the currently existing problems and development perspectives of CDs-based RTP materials was proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2021.122350DOI Listing
August 2021

Carbon dots for ratiometric fluorescence detection of morin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 29;256:119751. Epub 2021 Mar 29.

Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China. Electronic address:

The B,N dual-doped carbon dots (B,N-CDs) for ratiometric fluorescence detection the morin were prepared from sodium tetraborate and polyethyleneimine through the single-step hydrothermal method. The B,N-CDs exhibited the optimum excitation and emission wavelength at 340 nm and 467 nm, respectively. Interestingly, the intensities of emission peak at 467 nm of B,N-CDs reduced meanwhile a new peak emerged at 560 nm with the continuous addition of morin, which revealed the ratio fluorescence characteristic between F/F and morin concentration with the linearity range and detection limit of 14.5-32.5 μmol/L and 0.3 μmol/L (S/N = 3), respectively. The interference of common antibiotics and remedies could be ignored when the concentration of morin was detected by the B,N-CDs, which demonstrating the outstanding selectivity. Furthermore, the proposed fluorescence method is used to detect morin in urine with recoveries are 99.8-104.5%. The results of this research indicate the feasibility and practicality of B,N-CDs as an effective fluorescent probe for the determination of morin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119751DOI Listing
July 2021

Development of a piperazinyl-NBD-based fluorescent probe and its dual-channel detection for hydrogen sulfide.

Analyst 2021 Apr 2;146(7):2138-2143. Epub 2021 Mar 2.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

To selectively detect HS based on the thiolysis reaction of 7-nitro-1,2,3-benzoxadiazole (NBD), amines attracted increasing attention since NBD amine is regarded as a new HS reaction site. Herein, a novel fluorescent probe, triphenylamine piperazine NBD (TPA-Pz-NBD), was developed. The results showed that it exhibited high selectivity towards HS via fluorescence spectroscopy and solution color. Furthermore, TPA-Pz-NBD not only detected HS by a dual-channel, turn-on fluorescence signal at 500 nm and turn-off fluorescence signal at 545 nm, respectively, but also displayed a wide detection range of 0-125 μM. In addition, living cell imaging results indicated that TPA-Pz-NBD holds potential for the detection of intracellular HS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1an00054cDOI Listing
April 2021

Carbon Nanodots as a Multifunctional Fluorescent Sensing Platform for Ratiometric Determination of Vitamin B and "Turn-Off" Detection of pH.

J Agric Food Chem 2021 Mar 23;69(9):2836-2844. Epub 2021 Feb 23.

Institute of Environmental Science and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China.

In this work, we synthesized carbon nanodots (CNDs) by a one-pot hydrothermal method to carbonize precursors of dry carnation petals and polyethylenimine. The obtained CNDs possess favorable photostability, good biocompatibility, and excellent water solubility, which can serve as a dual-responsive nanosensor for the determination of vitamin B (VB) and pH. A unique ratiometric fluorescence resonance energy transfer probe was developed through a strong interaction between VB and surface moieties of CNDs. CNDs emitted at 470 nm; however, in the presence of VB, an enhanced emission peak was clearly observed at 532 nm. The value of / exhibits a stable response to the VB concentration from 0.35 to 35.9 μM with a detection limit of 37.2 nM, which has been used for VB detection in food and medicine samples and ratiometric imaging of VB in living cells with satisfying performance. In addition, the proposed CNDs also displayed pH-sensitive behavior and can be a turn-off fluorescent sensor to monitor pH. The fluorescent intensity at 470 nm is a good linear response against pH values from 3.6 to 8, affording the capability as a single-emissive nanoprobe for intracellular pH sensing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c07019DOI Listing
March 2021

Azithromycin detection in cells and tablets by N,S co-doped carbon quantum dots.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 29;252:119506. Epub 2021 Jan 29.

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China. Electronic address:

Azithromycin (AZM) is one of the most widely used antibiotics. AZM abuse is easy to cause great harm to human body, so developing a rapid and sensitive method to detect AZM is of great importance. Herein, 3-aminothiophenol as only reaction precursor, nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) were fabricated by one-step hydrothermal carbonization method. All characteristics demonstrate that N,S-CQDs possess good water solubility, high fluorescence stability and low cytotoxicity. Without being disturbed by amino acids and drugs, the most interesting finding is that AZM can efficiently quench the fluorescence of N,S-CQDs by a synergistic effect of electrostatic interaction and static quenching. A fluorescent probe for the detection of AZM was constructed with high selectivity and good sensitivity, achieving two linear ranges of 2.5-32.3 μM and 37.2-110 μM and a limit of detection of 0.76 µM. The proposed fluorescent method was used for the detection of AZM in cells with fulfilling results. More importantly, the fluorescent probe was successfully used to the detection of AZM in tablets and human urine with recovery rate and relative standard deviations of 98.2-104.8% and 0.04-3.46%, respectively, which was confirmed by the standard method of HPLC-UV. This finding illustrates the usefulness and feasibility of N,S-CQDs as an effective fluorescent probe for the detection of AZM in tablets and human urine, which is helpful for supervising and guiding pharmacy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119506DOI Listing
May 2021

Real-Time Monitoring Mitochondrial Viscosity during Mitophagy Using a Mitochondria-Immobilized Near-Infrared Aggregation-Induced Emission Probe.

Anal Chem 2021 02 4;93(6):3241-3249. Epub 2021 Feb 4.

Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, P. R. China.

Mitophagy plays a crucial role in maintaining intracellular homeostasis through the removal of dysfunctional mitochondria and recycling their constituents in a lysosome-degradative pathway, which leads to microenvironmental changes within mitochondria, such as the pH, viscosity, and polarity. However, most of the mitochondrial fluorescence viscosity probes only rely on electrostatic attraction and readily leak out from the mitochondria during mitophagy with a decreased membrane potential, thus easily leading to an inaccurate detection of viscosity changes. In this work, we report a mitochondria-immobilized NIR-emissive aggregation-induced emission (AIE) probe , which allows for an off-on fluorescence response to viscosity, thus enabling the real-time monitoring viscosity variation during mitophagy. This system consists of a cyanostilbene skeleton as the AIE active core and viscosity-sensitive unit, a pyridinium cation for the mitochondria-targeting group, and a benzyl chloride subunit that induces mitochondrial immobilization. As the viscosity increased from 0.903 cP (0% glycerol) to 965 cP (99% glycerol), exhibited an about 92-fold increase in fluorescence intensity at 650 nm, which might be attributed to the restriction of rotation and inhibition of twisted intramolecular charge transfer in a high viscosity system. We also revealed that could be well immobilized onto mitochondria, regardless of the mitochondrial membrane potential fluctuation. Most importantly, using , we have successfully visualized the increased mitochondrial viscosity during starvation or rapamycin-induced mitophagy in real time. All these features render a promising candidate to investigate mitophagy-associated dynamic physiological and pathological processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.0c04826DOI Listing
February 2021

A facile synthesis of long-wavelength emission nitrogen-doped carbon dots for intracellular pH variation and hypochlorite sensing.

Biomater Sci 2021 Mar 3;9(6):2255-2261. Epub 2021 Feb 3.

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, No. 92 Wucheng rd., Taiyuan 030006, China.

Intracellular pH and hypochlorite (ClO) concentration play an important role in life activities, so there is an urgent need to develop a valid strategy to monitor pH and ClO in biological systems with high sensitivity and specificity. In this study, we report long-wavelength emission nitrogen-doped carbon dots (N-CDs) and their potential applications in intracellular pH variation, ClO sensing and cell imaging. The N-CDs were prepared via a facile one-pot hydrothermal method of neutral red (NR) and glutamine (Gln). N-CDs exhibited a pH-sensitive response in the range of 4.0-9.0 and a good linear relationship in the range of 5.6-7.4, which indicated that N-CDs are an ideal agent for monitoring pH fluctuations in living cells. In addition, ClO was capable of reducing the photoluminescence of N-CDs based on static quenching. The linear range is 1.5-112.5 μM and 112.5-187.5 μM, and the LOD is 0.27 μM. Besides, the as-fabricated N-CDs have been smoothly achieved to monitor pH and ClO in PC-12 living cells due to their great biocompatibility and lower cytotoxicity, demonstrating their promising applications in the biomedical field. Compared with other CD-based methods, the as-proposed N-CDs have a longer fluorescence emission, which makes them potentially valuable in biological systems. The results pave a way towards the construction of long-wavelength carbon-based nanomaterials for fluorescence sensing and cell imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0bm02047hDOI Listing
March 2021

Orange emissive carbon nanodots for fluorescent and colorimetric bimodal discrimination of Cu and pH.

Analyst 2021 Mar 22;146(6):1907-1914. Epub 2021 Jan 22.

College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

We have facilely synthesized orange emissive carbon nanodots (O-CDs) via a hydrothermal method using citric acid and 5-aminosalicylic acid. The obtained O-CDs show the excellent characteristics of excitation independence, low toxicity, fabulous photostability and superior biocompatibility. Based on these captivating properties, as-prepared O-CDs have been successfully implemented as a multi-functional sensing platform for fluorescent and colorimetric bimodal recognition of Cu and pH. Upon adding Cu, the orange fluorescence of the O-CDs is evidently quenched with a linear range of 0 μM-300 μM, and a detection limit of 28 nM. Additionally, as the pH increases from 7.0 to 10.2, the O-CDs manifest an obvious decrease in orange fluorescence, which shows a pK value of 8.73 and excellent linearity in the pH range of 8.0-9.2. Appealingly, the laser confocal imaging of O-CD-stained cells demonstrates that the fluctuations of Cu and pH can be visualized in living cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an02243hDOI Listing
March 2021

One-step synthesis of red emission multifunctional carbon dots for label-free detection of berberine and curcumin and cell imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 6;251:119432. Epub 2021 Jan 6.

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. Electronic address:

In this work, the red emission multifunctional carbon dots (R-CDs) were prepared via one-pot hydrothermal strategy of neutral red (NR) and ethylenediamine (EDA) for the label-free detection of berberine and curcumin, cell imaging, and fluorescent flexible film. The as-fabricated R-CDs not only possess good water dispersibility and excellent fluorescence stability, but also were successfully employed as a photoluminescent nanoprobe for label-free monitoring of berberine (BRH) and curcumin (Cur) based on dynamic quenching and internal filter effect (IFE), respectively. More importantly, as-proposed R-CDs displayed outstanding cellular permeability and lower cytotoxicity for cellular applications, which was consistent with the results of confocal fluorescence imaging and cell viability measurement of SMMC7721 cells. Thus, the multifunctional R-CDs may provide a rich tool library for biosensing and cellular imaging reagent applications. Interestingly, R-CDs were also used to manufacture R-CDs/PVA composites as fluorescent flexible films. To the best of our knowledge, this is the first demonstration of a label-free multifunctional fluorescent nanoprobe for berberine and curcumin based on red emission CDs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119432DOI Listing
April 2021

Silk Fibroin-Confined Star-Shaped Decahedral Silver Nanoparticles as Fluorescent Probe for Detection of Cu and Pyrophosphate.

ACS Biomater Sci Eng 2020 05 23;6(5):2770-2777. Epub 2020 Apr 23.

School of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.

A green, one-step method for the fabrication of silk fibroin (SF) protected silver decahedral nanoparticles ([email protected]) has been developed. High-resolution transmission electron microscopy characterization demonstrated that the silver decahedral nanoparticles can provide more binding sites with (111) facets. Moreover, a facile strategy based on Cu mediated [email protected] was reported as an on-off-on fluorescent system for the detection of Cu, and the [email protected] can be also used for the determination of pyrophosphate ion (PO, PPi). The fluorescence of [email protected] was quenched by Cu as a complex formed between SF and Cu and was restored when PPi was introduced into the system due to the higher binding affinity between PPi and Cu. Herein, a novel [email protected] fluorescent probe for Cu and PPi detection is presented. The proposed assay shows a linear relationship at a Cu concentration range from 1 to 6 μM with a detection limit of 33.3 nM. This simple, reliable, selective, and environmentally friendly fluorescent probe also has a wide concentration range from 100 to 700 μM with a detection limit of 6.7 μM for PPi. The 16 types of anions lead to negligible changes in the [email protected] complexes, while restoring the fluorescence intensity of the complexes when added with PPi. Additionally, it is demonstrated that the [email protected] fluorescent assay works in real biological samples. It suggests that this proposed method has the potential for application in the clinical detection of Cu and PPi.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsbiomaterials.9b01862DOI Listing
May 2020

Facile Fabrication Route of Janus Gold-Mesoporous Silica Nanocarriers with Dual-Drug Delivery for Tumor Therapy.

ACS Biomater Sci Eng 2020 03 17;6(3):1573-1581. Epub 2020 Feb 17.

College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

Codelivery of drugs using multifunctional nanoplatforms with anisotropic properties can produce synergistic effects and improve the antitumor activity of the drugs. In this work, Janus gold-mesoporous silica nanoparticles have been successfully synthesized via the Pickering emulsion method. The obtained Janus nanoparticles were further selectively assembled with thiol-β-cyclodextrin as a drug delivery vehicle for paclitaxel on gold domains, while the other mesoporous silica side with a mesoporous structure served as a drug delivery vehicle for doxorubicin. These synthesized Janus nanoparticles possess pH and near-infrared (NIR) dual-responsive release properties. Furthermore, the tumor-bearing mice treated with dual-drug-loaded Janus nanoparticles showed obvious tumor inhibition than single-drug-loaded ones. Histological analysis reveals no pathological changes in the vital organs of the mice. The outcome demonstrated that dual-drug-loaded Janus gold-mesoporous silica nanoparticles possessed a high therapeutic efficiency and excellent biocompatibility both in vitro and in vivo and could be used as an effective candidate for cancer therapeutics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsbiomaterials.0c00042DOI Listing
March 2020

Ratiometric fluorescent sensors for sequential on-off-on determination of riboflavin, Ag and l-cysteine based on NPCl-doped carbon quantum dots.

Anal Chim Acta 2021 Feb 2;1144:1-13. Epub 2020 Dec 2.

Institute of Environmental Science, And School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, PR China. Electronic address:

The fluorescent sensor, especially ratiometric fluorescent sensor, is one of the most important applications for CQDs, which is becoming a research hotspot. Herein, carbon quantum dots co-doped with nitrogen, phosphorus and chlorine (NPCl-CQDs) were synthesized by acid-base neutralization reaction exothermic carbonization method. The as-fabricated NPCl-CQDs could emit blue fluorescence and possess excellent fluorescence properties. Based on the FRET, multifunctional and ratiometric fluorescent sensors for "on-off-on" sequential determination of riboflavin, Ag, and Cys with good selectivity and high sensitivity were established. The linear range of riboflavin, Ag, and Cys are 0.50-10.18 μM and 15.89-27.76 μM, 0.66-1.46 mM and 1.50-4.20 mM, and 0.01-0.15 μM and 0.15-0.36 μM with the limit of detection of 3.50 nM, 26.38 μM, and 0.96 nM, respectively. Furthermore, the sensors were successfully used to determine riboflavin, Ag, and Cys in tablets, river water, and human urine with the recoveries of 95.2-104.0%, 95.6-102.0%, and 94.8-106.4%, respectively. More importantly, the as-constructed "on-off-on" NPCl-CQDs-based ratiometric fluorescent sensors were applied for detecting riboflavin, Ag, and Cys in HeLa cells with satisfying results. The finding of this study shows the feasibility and effectiveness of the NPCl-CQDs as the available ratiometric fluorescent sensors for the determination of riboflavin, Ag, and Cys in real samples and living cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2020.11.054DOI Listing
February 2021

A red emitting fluorescent probe based on TICT for selective detection and imaging of HSA.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 31;250:119409. Epub 2020 Dec 31.

Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address:

A red emitting fluorescence probe, TPA-CPO, based on twisted intra-molecular charge transfer (TICT) was designed and synthesized. The spectra results displayed that TPA-CPO could sense HSA with excellent properties including significant fluorescence enhancement, long emission wavelength, large stokes shift, and wide linear range. The recognition mechanism was proved that TPA-CPO could bind to domain IB of HSA and its TICT process was suppressed by utilizing hydrophobic cavity and low polarity of HSA. TPA-CPO bind to domain IB instead of common drug sites of HSA could effectively avoid interference from most drugs. The selective response of TPA-CPO allowed quantitative detection of HSA with sensitivity limit of 13.65 µg/mL. What's more, it successfully achieved HSA imaging in HeLa cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2020.119409DOI Listing
April 2021

Visible-light-driven photoelectrochemical sensing platform based on BiOI nanoflowers/TiO nanotubes for detection of atrazine in environmental samples.

J Hazard Mater 2021 05 18;409:124894. Epub 2020 Dec 18.

Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, Shanxi 030006, PR China. Electronic address:

In this work, a visible-light-driven photoelectrochemical (PEC) sensing platform was developed based on BiOI nanoflowers/TiO nanotubes (BiOI NFs/TiO NTs) for detection of atrazine (ATZ). The BiOI NFs/TiO NTs p-n heterojunctions synthesized by decorating BiOI NFs on TiO NTs via simple hydrothermal approach exhibit strong visible-light absorption ability, high photocurrent response and PEC activity. Thus BiOI NFs/TiO NTs heterostructures were first explored to act as the photoelectrode for the immobilization of the anti-ATZ aptamer to develop a PEC sensing platform. The design PEC aptasensing platform exhibits prominent analytical performance for determination of ATZ with a low detection limit of 0.5 pM under visible-light irradiation, and displays good selectivity for ATZ in the control experiments. The superior behavior of the sensing platform could be ascribed to the design of the appropriate sensing material with tubular microstructure, excellent PEC response of the photoelectrode, and the large loading amount of aptamer. Meanwhile, the PEC sensing platform was used to determine ATZ in environmental samples and a satisfied result was obtained.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124894DOI Listing
May 2021

Nitrogen, sulfur, phosphorus, and chlorine co-doped carbon nanodots as an "off-on" fluorescent probe for sequential detection of curcumin and europium ion and luxuriant applications.

Mikrochim Acta 2021 Jan 5;188(1):16. Epub 2021 Jan 5.

Institute of Environmental Science, Shanxi University, Taiyuan, 030006, People's Republic of China.

Nitrogen, sulfur, phosphorus, and chlorine co-doped carbon nanodots (NSPCl-CNDs) were fabricated by acid-base neutralization and exothermic carbonization of glucose. The obtained NSPCl-CNDs possess excellent fluorescence properties and good biocompatibility. Curcumin (Cur) can dramatically quench the fluorescence of NSPCl-CNDs based on a synergistic effect of electrostatic interaction, inner filter effect, and static quenching, so a "turn-off" fluorescent probe for Cur detection was constructed with linear ranges of 0.24-13.16 μM and 13.62-57.79 μM. The LOD and LOQ of this fluorescent probe for Cur are 8.71 nM and 29.03 nM, respectively. More importantly, the fluorescence of the NSPCl-CNDs-Cur system can be recovered by europium ion (Eu), so a "turn-on" fluorescent probe for Eu determination was established. The linear range, LOD, and LOQ for the detection of Eu were 2.36-32.91 μΜ, 73.29 nM, and 244.30 nM, respectively. The proposed fluorescence methods were successfully utilized for Cur and Eu determination in real samples with recoveries in the range 95.64-104.13% and 97.06-98.70%, respectively. Furthermore, the qualitative analysis of Cur can be realized by reagent strips with satisfying results. Finally, the as-constructed "off-on" fluorescent probe was successfully used to sequentially analyze Cur and Eu at the cellular level. This method is simple and easy to implement, manifesting that NSPCl-CNDs have potential application value in fluorescent probing, food and drug testing, environmental monitoring, and cellular labeling. Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-020-04618-8DOI Listing
January 2021

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen.

Analyst 2021 Feb;146(4):1340-1345

College of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.

Prostate specific antigen (PSA) has been considered as the most potential serological biomarker for the early stage detection of prostate cancer. Here, a label-free fluorescence aptasensing strategy for detecting PSA based on hybridization chain reaction (HCR) and G-quadruplex DNAzymes has been developed. This designed strategy consists of three DNA probes, aptamer probe (AP), hairpin probe 1 (H1) and hairpin probe 2 (H2). In the presence of target PSA, the aptamer sequences in AP specifically recognized PSA to form a PSA-aptamer complex, causing an AP conformation change and thus releasing the initiator, which triggered the chain-like assembly of H1 and H2 that yielded extended nicked double-stranded DNA through HCR. Upon the addition of hemin, the G-rich segments at the end of H1 and H2 self-assembled into the peroxidase-mimicking hemin/G-quadruplex DNAzymes, which catalyzed the hydrogen peroxide-mediated oxidation of thiamine to give a fluorescence signal dependent on the concentration of PSA. Under optimal conditions, a limit of detection of 0.05 nM and a linear range from 0.1 nM to 1 nM (R2 = 0.9942) were achieved by this assay. In addition, other interfering proteins, such as IgG, AFP and CEA, did not produce any significant change in the fluorescence intensity response, indicating good selectivity of this sensor for PSA detection. Finally, this proposed aptasensor was successfully used for diluted serum samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an02188aDOI Listing
February 2021

Fe and intracellular pH determination based on orange fluorescence carbon dots co-doped with boron, nitrogen and sulfur.

Mater Sci Eng C Mater Biol Appl 2021 Jan 3;118:111478. Epub 2020 Sep 3.

Institute of Environmental Science, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

The fluorescent boron, nitrogen and sulfur co-doped carbon dots (BNSCDs) were prepared by simple hydrothermal reaction of 4-carboxyphenylboronic acid and 2,5-diaminobenzenesulfonic acid at 200 °C for 8 h. The fluorescence of the BNSCDs could be quenched by Fe based on the electron transfer between Fe and BNSCDs, so a label-free, good selectivity and high sensitivity method for Fedetermination was established with linear range and LOD of 1.5-692 μmol/L and 87 nmol/L, respectively. And then the fluorescent probe was employed for detection of Fe in tap water, coal gangue, fly ash and food samples successfully. Moreover, the as-prepared BNSCDs could serve as a novel pH fluorescent probe in the range of pH 1.60-7.00, which could be attributed to the proton transfer of carboxyl groups on the surface of BNSCDs. More importantly, the pH fluorescent probe possesses fast, real-time and low toxicity, applying for intracellular pH fluorescence imaging in HIC, HIEC, LO2 and SMMC7721 cells. In view of its simplicity, timely response and outstanding compatibility, the as-fabricated BNSCDs show the potential applications in water quality and solid waste monitoring, food detection, real-time measuring of intracellular pH change in vitro.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2020.111478DOI Listing
January 2021

Alizarin-based molecular probes for the detection of hydrogen peroxide and peroxynitrite.

Analyst 2021 Jan 19;146(2):509-514. Epub 2020 Nov 19.

College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, P. R. China.

Phenol fluorophores are a large family of fluorophores, which have attracted more and more attention in the design of probes. Using the self-assembly of aromatic boronic acid with Alizarin Red S (ARS) and Gallein (GAL), the novel chemosensors ARS-CBA and GAL-CBA were provided for hydrogen peroxide (HO), which demonstrated their ability to detect HO with indicator displacement assay (IDA) by colorimetric and electrochemical measurements. After ARS-CBA and GAL-CBA reacted with HO, the systems displayed a red-shifted visible color change in aqueous media and off-on electrochemical signals showing generation of phenol. The chemosensor ARS-CBA also had good performance in fluorometric measurements and turn-off fluorescent response indicated removal of aromatic boronic acid. In addition, a designed near-infrared (NIR) dual-modal fluorescent probe alizarin blue S (ABS) was used for peroxynitrite (ONOO) with a visible colorimetric change in dimethyl sulfoxide (DMSO) and "on-off" fluorescent response indicating the oxidation of hydroxyl. The flexible Phenol fluorophores are allowed to prepare multiple fluorescent probes towards HO or ONOO for environmental and physiological applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an01805hDOI Listing
January 2021

Facile synthesis of orange fluorescence multifunctional carbon dots for label-free detection of vitamin B and endogenous/exogenous peroxynitrite.

J Hazard Mater 2021 04 29;408:124422. Epub 2020 Oct 29.

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. Electronic address:

In this work, orange emission fluorescent multifunctional carbon dots (O-CDs) were designed for the label-free detection of vitamin B (VB),endogenous/exogenous peroxynitrite (ONOO) sensing, cell imaging, and fluorescent flexible film preparation. The O-CDs with excitation-independent were prepared using safranine T and ethanol as precursors via one-step hydrothermal process. VB was utilized as a quencher to quench the fluorescence of O-CDs due to the internal filtration effect (IFE). Two-segment linear ranges are 1-65 μM and 70-140 μM, and the detection limit was calculated as 0.62 μM. Besides, ONOO can reduce the fluorescence intensity of O-CDs based on static quenching (SQ). The linear ranges are 0.3-9 μM and 9-48 μM, and the detection limit was 0.06 μM. Moreover, the O-CDs were exploited as a cellular imaging reagent for intracellular VB and endogenous/exogenous ONOO imaging owing to its great biocompatibility, low toxicity and strong photostability. These results indicate that O-CDs have the potential to be used as a sensitive fluorescence probe to rapidly monitor VB and endogenous/exogenous ONOO with high selectivity in living cells. Also, the as-proposed O-CDs can be employed to fabricate O-CDs/PVA composites as fluorescent flexible films. All of the above prove that the O-CDs present great prospect in multiple applications such as biosensing, cellular labeling, biomedical optical imaging, and fluorescent films.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124422DOI Listing
April 2021

A benzothiazolium-based fluorescent probe with ideal pK for mitochondrial pH imaging and cancer cell differentiation.

J Mater Chem B 2020 12 30;8(46):10586-10592. Epub 2020 Oct 30.

College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.

A mitochondrial pH sensing fluorescent probe namely 2-(2-(6-hydroxynaphthalen-2-yl)vinyl)-3-(6-(triphenyl-phosphonio)hexyl)benzothiazol-3-ium bromide (HTBT2) was designed and facilely synthesized via the Knoevenagel condensation reaction. HTBT2 displayed a linear fluorescence enhancement at 612 nm in response to pH changes between 8.70 and 7.20. The pK value was determined to be 8.04 ± 0.02, which might be ideal for mitochondrial pH (pH∼8.0) detection. HTBT2 also exhibited a remarkable large Stokes shift of 176 nm, which could diminish the interference of excitation light. The results of live cell imaging studies suggested that HTBT2 showed excellent targeting ability for mitochondria. Importantly, it was successfully applied to visualize mitochondrial pH changes in live cells and differentiate the pH difference between cancer cell lines and normal cell lines. Our results consistently supported that HTBT2 held practical promise for the investigation of physiological processes related to pH changes and clinical potential for cancer cell differentiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb01253jDOI Listing
December 2020

Facile synthesis of ultrahigh fluorescence N,S-self-doped carbon nanodots and their multiple applications for HS sensing, bioimaging in live cells and zebrafish, and anti-counterfeiting.

Nanoscale 2020 Oct;12(39):20482-20490

Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

Green-emissive N,S-self-doped carbon nanodots (N,S-self-CNDs) with an ultrahigh fluorescence (FL) quantum yield (QY) of 60% were synthesized using methyl blue as the only source by a facile hydrothermal approach. The -NH- and -SOx- groups of methyl blue were simultaneously used as nitrogen and sulfur co-dopants to dope into CNDs. The prepared N,S-self-CNDs have an extremely large Stokes shift (∼130 nm) and excitation-independent fluorescence, and are demonstrated to have multiple applications for H2S sensing, bioimaging and anti-counterfeiting. Taking advantage of their excellent optical properties, N,S-self-CNDs could act as a label-free nanoprobe for the detection of H2S. The FL of N,S-self-CNDs could be significantly quenched by H2S because of dynamic quenching, along with excellent selectivity toward H2S from 0.5-15 μM with a detection limit of 46.8 nM. They were successfully employed for the analysis of H2S content in actual samples. Additionally, the nanoprobe was extended to bioimaging in both living PC12 cells and zebrafish, and monitoring H2S in live cells. Furthermore, N,S-self-CNDs have been used to prepare highly fluorescent polymer films by incorporating N,S-self-CNDs in polyvinyl alcohol (PVA). The as-prepared N,S-self-CNDs/PVA films show a prominent dual-mode FL property, implying that they are potential nanomaterials in the anti-counterfeiting field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr04649cDOI Listing
October 2020

Gold nanoparticles decorated bimetallic CuNi-based hollow nanoarchitecture for the enhancement of electrochemical sensing performance of nitrite.

Mikrochim Acta 2020 09 17;187(10):572. Epub 2020 Sep 17.

School of Chemistry and Chemical Engineering, and Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.

Gold nanoparticles (AuNPs) decorated bimetallic CuNi-based hollow nanoarchitecture (CNHN) are reported for the first time as a nonenzymatic sensor for the quantification of nitrite in neutral solution . The CNHN was prepared via a convenient calcining routine using the bimetallic CuNi-MOFs as a coprecursor. The unique chemical structure of hollow CNHN with high specific surface area and abundant terminal amino groups effectively avoid the aggregation of AuNPs and facilitate the subsequent adsorption of nitrite. The Au/CNHN exhibited high electrocatalytic activity towards nitrite oxidation due to the synergetic catalytic effect of AuNPs and CNHN. Chronoamperometric detection of nitrite at the Au/CNHN/GCE achieved a lower linear calibration range of 0.05 to 1.15 mM, with an LOD of 0.017 μM compared with previous reports. The proposed method obtained satisfactory recoveries for nitrite determination in practical applications, which was verified by UV-Vis spectrophotometry. The prepared sensor based on Au/CNHN featured favorable selectivity and stability, which provides a promising approach for real sample analysis. Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-020-04545-8DOI Listing
September 2020

Graphene quantum dots wrapped square-plate-like MnO nanocomposite as a fluorescent turn-on sensor for glutathione.

Talanta 2020 Nov 23;219:121180. Epub 2020 May 23.

College of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, PR China. Electronic address:

Benefiting from their excellent optical absorption and electron transfer properties, manganese dioxide nanomaterials have been widely applied for fluorescence-based nanosensors. In this work, graphene quantum dots (GQDs) wrapped square-plate-like MnO nanocomposite was synthesized from potassium permanganate via an in situ redox procedure under ultrasonication with poly(allylamine hydrochloride) (PAH) being involved. Through an effective fluorescence resonance energy transfer (FRET) process and inner filter effect (IFE) between GQDs and MnO, the fluorescence of GQDs was quenched. Furthermore, the introduction of glutathione (GSH) decomposed MnO and caused fluorescence recovery of GQDs. Therefore, a MnO mediated nanosensor was established for fluorescent turn-on sensing of GSH. A satisfactory linear range was found to be 0.07-70 μM and the detection limit was as low as 48 nM. Besides, the fluorescent recognition of cancer cells using GQDs-MnO nanocomposite was achieved because of the obviously higher GSH content in cancer microenvironment than normal cells. This nanosensor was constructed directly in GQDs solution in the presence of PAH without the complicated modifications or connections, making it a facile and novel nanosensor for GSH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2020.121180DOI Listing
November 2020