Publications by authors named "Shaolong Huang"

12 Publications

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circRNA_0006470 promotes the proliferation and migration of gastric cancer cells by functioning as a sponge of miR-27b-3p.

Neoplasma 2021 Oct 11. Epub 2021 Oct 11.

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People's Hospital of Southern Medical University, Dongguan, Guangdong, China.

Cancer pathogenesis is influenced by epigenetic alterations mediated by circular RNAs (circRNAs). In this study, we aimed to investigate the regulatory mechanisms and cytological function of hsa_circ_0006470/miR-27b-3p in gastric cancer (GC). CircRNA and microRNA expression in cancer cells were measured by the qRT-PCR method. A dual-luciferase reporter assay was performed to validate the binding of hsa_circ_0006470 with miR-27b-3p. hsa_circ_0006470 was silenced in AGS cells, and proliferation, migration, and invasion were tested via the CCK-8 assay and Transwell system, respectively. The autophagy in GC cells was assessed by marker protein detection and transmission electron microscope. The results showed that hsa_circ_0006470 expression was significantly elevated in GC cells, which was mainly distributed in cytoplasmic components and could directly bind with miR-27b-3p in GC cells. Silencing of hsa_circ_0006470 repressed cell proliferation, migration, and invasion, which may be through regulating miR-27b-3p/Receptor tyrosine kinase-like orphan receptor 1 (ROR1). Silencing of hsa_circ_0006470 also elevated LC3II and Beclin-1 and suppressed p62 protein abundances, which subsequently induced autophagy in AGS cells. Furthermore, we found that hsa_circ_0006470 promotes phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PI3KCA) expressing by sponging miR-27b-3p. In conclusion, hsa_circ_0006470 promoted GC cell proliferation and migration through targeting miR-27b-3p and suppressing autophagy machinery.
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http://dx.doi.org/10.4149/neo_2021_210222N235DOI Listing
October 2021

Prognostic Nutritional Index (PNI) in Patients With Breast Cancer Treated With Neoadjuvant Chemotherapy as a Useful Prognostic Indicator.

Front Cell Dev Biol 2021 30;9:656741. Epub 2021 Mar 30.

Department of Breast Surgical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: Prognostic nutritional index (PNI), calculated as serum albumin (ALB) (g/L) + 5 × total lymphocyte count (10/L), is initially used to evaluate nutritional status in patients undergoing surgery and may evaluate the therapeutic effects and predict the survival of various solid tumors. The present study aimed to evaluate the potential prognostic significance of PNI in breast cancer patients receiving neoadjuvant chemotherapy (NACT).

Methods: A total of 785 breast cancer patients treated with neoadjuvant chemotherapy were enrolled in this retrospective study. The optimal cutoff value of PNI by receiver operating characteristic curve stratified patients into a low-PNI group (<51) and a high PNI group (≥51). The associations between breast cancer and clinicopathological variables by PNI were determined by chi-square test or Fisher's exact test. Kaplan-Meier plots and log-rank test were used to evaluate the clinical outcomes of disease-free survival (DFS) and overall survival (OS). The prognostic value of PNI was analyzed by univariate and multivariate Cox proportional hazards regression models. The toxicity of NACT was accessed by the National Cancer Institute Common Toxicity Criteria (NCI-CTC).

Results: The results indicated that PNI had prognostic significance by an optimal cutoff value of 51 on DFS and OS in univariate and multivariate Cox regression survival analyses. Breast cancer patients with a high PNI value had longer DFS and OS than those with a low PNI value [47.64 . 36.60 months, < 0.0001, hazard ratio (HR) = 0.264, 95%CI = 0.160-0.435; 73.61 . 64.97 months, < 0.0001, HR = 0.319, 95%CI = 0.207-0.491, respectively]. Furthermore, the results indicated that patients with high PNI had longer DFS and OS than those with low PNI in early stage and advanced breast cancer, especially in advanced breast cancer. The mean DFS and OS times for breast cancer patients with high PNI by the log-rank test were longer than in those with low PNI in different molecular subtypes. Moreover, the mean DFS and OS times in patients with high PNI by the log-rank test were longer than in those patients with low PNI without or with lymph vessel invasion. The common toxicities after neoadjuvant chemotherapy were hematologic and gastrointestinal reaction, and the PNI had no significance on the toxicities of all enrolled patients, except in anemia, leukopenia, and myelosuppression.

Conclusion: Pretreatment PNI with the advantages of being convenient, noninvasive, and reproducible was a useful prognostic indicator for breast cancer patients receiving neoadjuvant chemotherapy and is a promising biomarker for breast cancer on treatment strategy decisions.
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http://dx.doi.org/10.3389/fcell.2021.656741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042235PMC
March 2021

Combined targeting of vascular endothelial growth factor C (VEGFC) and P65 using miR-27b-3p agomir and lipoteichoic acid in the treatment of gastric cancer.

J Gastrointest Oncol 2021 Feb;12(1):121-132

Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Dongguan Third Clinical Hospital of Guangdong Medical University, Dongguan, China.

Background: Gastric cancer is the second leading cancer-related mortality worldwide and more effective treatment strategies are urgently needed to combat the disease. Using lipoteichoic acid (LTA) and miR-27b-3p agomir, we aimed to assess the efficacy of this combination of therapies in treating gastric cancer.

Methods: The RNA levels of miR-27b-3p, FOXO3, MET, KRAS, vascular endothelial growth factor C (VEGFC), TSC1, and P65 were analyzed by quantified-PCR (Q-PCR) and the cell viability of AGS cells was analyzed by MTT. Confirm Luciferase reporter assays were used to explore the putative miR-27b-3p binding sites and Western blot analyzed the protein level of GAPDH, VEGFC, P65, AKT, and phosphorylated-AKT (p-AKT). The level of P65 in both the cytoplasm and nucleus of AGS cells was visualized by immunofluorescence assay. Subcutaneous xenograft models of gastric cancer were established, and mice were treated with miR-27b-3p agomir, LTA, or both. Hematoxylin-eosin staining and Ki-67 immunohistochemistry analysis of tumor tissues were then performed.

Results: The results showed that the decreased expression of miR-27b-3p in gastric cancer cell lines inhibited the viability of AGS cells, and VEGFC was confirmed as the target of miR-27b-3p. In addition, ectopic expression of miR-27b-3p significantly inhibited the AKT pathway in AGS and N87 cells, and LTA suppressed the proliferation of gastric cancer cells by inhibiting the NF-κB pathway. In an established xenograft model, both miR-27b-3p agomir alone and LTA treatment alone inhibited tumor growth and treatment which combined the two showed an even stronger inhibitory effect.

Conclusions: Taken together, the combined use of LTA and miR-27b-3p agomir exhibited a synergistic effect in the treatment of gastric cancer.
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http://dx.doi.org/10.21037/jgo-21-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944161PMC
February 2021

Variation in Breast Cancer Subtype Incidence and Distribution by Race/Ethnicity in the United States From 2010 to 2015.

JAMA Netw Open 2020 10 1;3(10):e2020303. Epub 2020 Oct 1.

Department of Breast Surgical Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Chaoyang District, Beijing, China.

Importance: Breast cancer is the most commonly diagnosed cancer and the leading cause of death in women worldwide. Yet the racial/ethnic disparity in incidences and distributions of breast cancer remains largely unknown.

Objective: To examine the racial/ethnic patterns associated with the incidence of the subtypes of breast cancer and distribution of patients across clinicopathological variables.

Design, Setting, And Participants: This population-based cohort study analyzed data from the Surveillance, Epidemiology, and End Results (SEER) database, which collected data from 18 SEER cancer registries that identified patients with breast cancer in the US diagnosed between January 1, 2010, and December 31, 2015. The inclusion criteria were (1) female patients with primary unilateral breast cancer who underwent surgical treatment; (2) record of estrogen receptor, progesterone receptor, and ERBB2 status; (3) record of medical history and histological subtype of the specified tumor location; and (4) data on patient race/ethnicity, lateral tumor position, tumor size, tumor TNM stage, and number of tumors. Incidence and distribution rates were identified and compared for different molecular subtypes, histological grades, pathological patterns, T stages, TNM stages, and tumor sites of breast cancers for each racial/ethnic group. Patient race/ethnicity was classified as follows: non-Hispanic White, Hispanic White, Black, Asian/Pacific Islander, American Indian/Alaskan Native, and unknown. Data were analyzed from January 1, 2010, to December 31, 2015.

Main Outcomes And Measures: Incidence rates of 4 molecular subtypes: hormone receptor (HR)-positive and ERBB2-negative, HR-positive and ERBB2-positive, HR-negative and ERBB2-positive, and triple-negative breast cancer (TNBC). Data on distribution by histological grades (grades 1-4 and unknown), pathological patterns, T stages, TNM stages, and tumor sites was also extracted. Annual age-standardized incidence rates and incidence rate ratios (IRRs) were calculated with 95% CIs. Race/ethnicity case-to-case odds ratios were estimated using polytomous regression.

Results: A total of 239 211 women with breast cancer were analyzed (median [interquartile range]) age, 60 [50-69] years). The annual incidence rate of all breast cancers was 31.3 (95% CI, 31.2- 31.5) per 100 000 people in non-Hispanic White women (the reference group), which was higher compared with the incidence among Black women (IRR, 1.04; 95% CI, 1.02-1.05; P < .001). The incidence rates were also lower in Asian/Pacific Islander (IRR, 0.90; 95% CI, 0.89-0.92; P < .001), American Indian/Alaskan native (IRR, 0.82; 95% CI, 0.81-0.83; P < .001), and Hispanic White women (IRR, 0.79; 95% CI, 0.75-0.83; P < .001). In Black patients, the incidences of the HR-positive and ERBB2-positive (IRR, 1.12; 95% CI, 1.08-1.16; P < .001), HR-negative and ERBB2-positive (IRR, 1.46; 95% CI, 1.38-1.54; P < .001), and TNBC (IRR, 2.07; 95% CI, 2.01-2.14; P < .001) subtypes were higher than those in non-Hispanic White patients, but the incidence of the HR-positive and ERBB2-negative subtype in Black women was lower (IRR, 0.86; 95% CI, 0.84-0.87; P < .001). The incidences of histological grade 1 (IRR, 0.75; 95% CI, 0.73-0.78; P < .001) and grade 2 (IRR, 0.91; 95% CI, 0.89-0.93; P < .001) were lower in Asian/Pacific Islander vs non-Hispanic White patients. Non-Hispanic White patients had a higher proportion of lobular carcinoma (9.7% [n = 15 718]) and tubular adenocarcinoma (0.6% [n = 997]) than Black (7.2% [n = 1933]; 0.3% [n = 81]), Asian/Pacific Islander (5.7% [n = 1202]; 0.3% [n = 55]), Hispanic White (7.2% [n = 1985]; 0.3% [n = 88]), and American Indian/Alaskan Native patients (7.2% [n = 101]; 0.4% [n = 5]).

Conclusions And Relevance: This cohort study found notable disparities in incidences and proportions of different molecular subtypes, histological grades, pathological patterns, T stages, TNM stages, and tumor sites associated with race/ethnicity. The findings suggest that combining epidemiologic with genomic and molecular profiling data warrants further research.
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http://dx.doi.org/10.1001/jamanetworkopen.2020.20303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573683PMC
October 2020

Non-precious molybdenum nanospheres as a novel cocatalyst for full-spectrum-driven photocatalytic CO reforming to CH.

J Hazard Mater 2020 Jul 19;393:122324. Epub 2020 Feb 19.

Shenzhen Key Laboratory of Laser Engineering, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China. Electronic address:

Photocatalytic CO reforming is considered to be an effective method for clean, low-cost, and environmentally friendly reduction and conversion of CO into hydrocarbon fuels by utilizing solar energy. However, the low separation efficiency of charge carriers and deficient reactive sites have severely hampered the efficiency of the photocatalytic CO reforming process. Therefore, cocatalysts are usually loaded onto the surface of semiconductor photocatalysts to reduce the recombination of charge carriers and accelerate the rates of surface reactions. Herein, molybdenum (Mo) nanospheres are proposed as a novel non-precious cocatalyst to enhance the photocatalytic CO reforming of g-CN significantly. The Mo nanospheres boost the adsorption of CO and activate the surface COvia a photothermal effect. The time-resolved fluorescence decay spectra reveals that the lifetime of photo-induced charge carriers is prolonged by the Mo nanospheres, which guarantees the migration of charge carriers from g-CN to Mo nanospheres. Unexpectedly, Mo loaded g-CN can effectively utilize a wide spectral range from UV to near-infrared region (NIR, up to 800 nm). These findings highlight the potential of Mo nanospheres as a novel cocatalyst for photocatalytic CO reforming to CH.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122324DOI Listing
July 2020

Ce-Doped Graphitic Carbon Nitride Derived from Metal Organic Frameworks as a Visible Light-Responsive Photocatalyst for H Production.

Nanomaterials (Basel) 2019 Oct 30;9(11). Epub 2019 Oct 30.

Center for Advanced Material Diagnostic Technology, Shenzhen Technology University, Shenzhen 518118, China.

Novel fibrous graphitic carbon nitride (g-CN) derivatives prepared from metal organic frameworks (MOFs) were doped with Ce (Ce-CN) as photocatalytic materials. Ce-CN was characterized using various techniques, revealing its high specific surface area, excellent photocatalytic activity, and stability for H evolution under visible light irradiation. The fluorine modified samples show superior photocatalytic activity under visible light irradiation, which is due to the presence of more active sites and enhanced absorption of solar energy. This work provides a new synthetic route for MOF-derived g-CN that can be doped with different metal ions. The fluorine modified Ce-CN is an efficient photocatalyst with potential for many applications related to energy and the environment.
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http://dx.doi.org/10.3390/nano9111539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915336PMC
October 2019

Enhanced Photocatalytic CO Reduction in Defect-Engineered Z-Scheme WO /g-CN Heterostructures.

ACS Omega 2019 Sep 11;4(13):15593-15599. Epub 2019 Sep 11.

Shenzhen Key Laboratory of Laser Engineering, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China.

Oxygen vacancy-modified WO nanorods composited with g-CN have been synthesized via the chemisorption method. The crystalline structure, morphology, composition, band structure, and charge separation mechanism for WO /g-CN heterostructures are studied in detail. The g-CN nanosheets are attached on the surface of WO nanorods. The Z-scheme separation is confirmed by the analysis of generated hydroxyl radicals. The electrons in the lowest unoccupied molecular orbital of g-CN and the holes in the valence band of WO can participate in the photocatalytic reaction to reduce CO into CO. New energy levels of oxygen vacancies are formed in the band gap of WO, further extending the visible-light response, separating the charge carriers in Z-scheme and prolonging the lifetime of electrons. Therefore, the WO /g-CN heterostructures exhibit much higher photocatalytic activity than the pristine g-CN.
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http://dx.doi.org/10.1021/acsomega.9b01969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761746PMC
September 2019

Multifunctional Zn-Al layered double hydroxides for surface-enhanced Raman scattering and surface-enhanced infrared absorption.

Dalton Trans 2019 Jan;48(2):426-434

Shenzhen Key Laboratory of Laser Engineering, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, P. R. China.

Surface-enhanced Raman scattering (SERS) and surface-enhanced infrared absorption (SEIRA) are complementary techniques, and both provide fingerprint structural information on various materials with a high sensitivity. Herein, Zn-Al layered double hydroxides (LDHs) are proposed for the first time as highly sensitive and uniform substrates for both SERS and SEIRA. Zn-Al LDHs show a remarkable SERS effect with an enhancement factor (EF) as high as 1.637 × 104 by using 4-mercaptobenzoic acid (4-MBA) as the probe molecule, where the charge transfer and hydrogen bonds are believed to result in the SERS effect. Interestingly, Zn-Al LDHs also exhibit SEIRA by using 4-methoxybenzenethiol (4-MTP), where the resultant substrates possess excellent long-term stability. This study not only presents a facile route to fabricate LDH materials, but also provides a novel substrate that can be used in both SERS and SEIRA.
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http://dx.doi.org/10.1039/c8dt03807dDOI Listing
January 2019

Fabrication of ZnO/Red Phosphorus Heterostructure for Effective Photocatalytic H₂ Evolution from Water Splitting.

Nanomaterials (Basel) 2018 Oct 15;8(10). Epub 2018 Oct 15.

Shenzhen Key Laboratory of Laser Engineering, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

Photocatalysis is a green technique that can convert solar energy to chemical energy, especially in H₂ production from water splitting. In this study, ZnO and red phosphorus (ZnO/RP) heterostructures were fabricated through a facile calcination method for the first time, which showed the considerable photocatalytic activity of H₂ evolution. The photocatalytic activities of heterostructures with different ratios of RP have been investigated in detail. Compared to bare ZnO, ZnO/RP heterostructures exhibit a 20.8-fold enhancement for H₂ production and furthermore overcome the photocorrosion issue of ZnO. The improved photocatalytic activities highly depend on the synergistic effect of the high migration efficiency of photo-induced electron⁻hole pairs with the inhibited charge carrier recombination on the surface. The presented strategy can also be applied to other semiconductors for various optoelectronics applications.
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http://dx.doi.org/10.3390/nano8100835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215150PMC
October 2018

Stannous oxide promoted charge separation in rationally designed heterojunction photocatalysts with a controllable mechanism.

Dalton Trans 2018 Sep;47(36):12734-12741

Shenzhen Key Laboratory of Laser Engineering, Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics Engineering, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, People's Republic of China.

Due to the sluggish mobility of holes, the low charge-separation rate remains an intrinsic issue that limits further increase of the photocatalytic conversion efficiency. Herein, we proposed an in situ hydrothermal method to expedite the charge transfer with enhanced photocatalytic H2 evolution rate and photodegradation activities via introducing SnO microplates into TiO2. As compared to bare TiO2, the SnO/TiO2 heterojunction achieves remarkable 470% and 150% higher efficiency for the photocatalytic H2 evolution rate and photodegradation of rhodamine B, respectively. In particular, it is demonstrated that the charge transfer mechanism of SnO/TiO2 can be switched from the Z-scheme to type II by Pt loading, leading to a significant enhancement of photocatalytic performances. Furthermore, the photocatalytic H2 evolution activities of ZnO and C3N4 can also be improved by introducing SnO via simple mechanical mixing. This work provides not only a new versatile stimulant for enhancing photocatalytic activities but also in-depth understanding of the charge transfer mechanism of heterointerfaces of semiconductors.
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http://dx.doi.org/10.1039/c8dt02708kDOI Listing
September 2018

In-Situ Conversion of ZnO/Ni₃ZnC/CNT Composite from NiZn Bimetallic MOF Precursor with Enhanced Electromagnetic Property.

Nanomaterials (Basel) 2018 Aug 7;8(8). Epub 2018 Aug 7.

Shenzhen Key Laboratory of Laser Engineering, Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics Engineering, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.

ZnO/Ni₃ZnC/x% CNT (x = 0, 2, 5, 10) urchin-like structures have been synthesized using a facile method based on metal-organic frameworks (MOFs) and applied as the electromagnetic absorber. The results of the experiments demonstrate that the content of multi-wall carbon nanotubes (MWCNT) has a great influence on the absorbing performance of the hybrid material. Conduction loss, interfacial polarization and ferromagnetic resonance all contribute to the electromagnetic absorption. The urchin-like ZnO/Ni₃ZnC/5% CNT composite presents excellent absorbing properties. When the filler loading of ZnO/Ni₃ZnC/5% CNT composite in paraffin matrix is only 10 wt.%, a minimum reflection loss of -33.2 dB is achieved at a layer thickness of 4.9 mm.
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http://dx.doi.org/10.3390/nano8080600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116268PMC
August 2018

Synthesis of TiO-N/SnO heterostructure photocatalyst and its photocatalytic mechanism.

J Colloid Interface Sci 2017 Jan 28;486:176-183. Epub 2016 Sep 28.

The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Institute of Applied Physics and School of Physics, Nankai University, Tianjin 300457, PR China. Electronic address:

A series of TiO-N/SnOX heterostructure photocatalysts were synthesized by a hydrolysis-deposition method. The structure, existing states of N and SnO heterostructure at the interface of TiO-N/SnOX were studied by EADX, XRD, Raman, FT-IR, XPS, and HRTEM. The band structure is investigated by both theoretical calculation and experiment characterization. It was found that the introduction of NO surface species and SnO nanoparticles would enhance the absorption in visible region, increase reactive oxidative species and separate photogenerated electrons and holes efficiently. Therefore, the photocatalytic activity is improved significantly for TiO-N/SnOX, compared with TiO-N and TiO under visible and UV light irradiation. This work may offer a new strategy to fabricate new photocatalyst with high photocatalytic performance.
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http://dx.doi.org/10.1016/j.jcis.2016.09.072DOI Listing
January 2017
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