Publications by authors named "Shaojie Zhang"

116 Publications

A dandelion polysaccharide and its selenium nanoparticles: Structure features and evaluation of anti-tumor activity in zebrafish models.

Carbohydr Polym 2021 Oct 22;270:118365. Epub 2021 Jun 22.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

In this study, an inulin fructan (TMP50-2) with moderate anti-tumor activity was obtained from dandelion. To further improve the anti-tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and Tween 80 were tightly wrapped on the surface of SeNPs by forming CO⋯Se bonds or through hydrogen bonding interaction (OH⋯Se). In vitro anti-tumor assay showed that Tw-TMP-SeNP treatment could significantly inhibit the proliferation of cancer cells (HepG2, A549, and HeLa) in a dose-dependent manner, while HepG2 cells were more susceptible to Tw-TMP-SeNP with an IC value of 46.8 μg/mL. The apoptosis induction of HepG2 cells by Tw-TMP-SeNP was evidenced by increasing the proportion of apoptotic cells ranging from 12.5% to 27.4%. Furthermore, in vivo zebrafish model confirmed the anti-tumor activity of Tw-TMP-SeNP by inhibiting the proliferation and migration of tumor cells as well as the angiogenesis of zebrafish embryos.
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http://dx.doi.org/10.1016/j.carbpol.2021.118365DOI Listing
October 2021

Construction and antitumor activity of selenium nanoparticles decorated with the polysaccharide extracted from Citrus limon (L.) Burm. f. (Rutaceae).

Int J Biol Macromol 2021 Jul 29;188:904-913. Epub 2021 Jul 29.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 3003350, People's Republic of China. Electronic address:

Selenium nanoparticles (SeNPs), a potential cancer therapeutic agent, have attracted widespread attention owing to their high bioavailability and remarkable anticancer activity. Nevertheless, the poor water solubility and dispersibility of SeNPs seriously limit their applications. In the present study, we synthesized stable and individual spherical selenium nanoparticles (CL90-Tw-SeNP2) with an average diameter of approximately 79 nm using a polysaccharide extracted from Citrus limon (CL90) and Tween-80 as the decorator and stabilizers. The proportion of selenium in CL90-Tw-SeNP2 was 10.6%. CL90-Tw-SeNP2 possessed high stability and good dispersion in water for more than three months. The subsequent biological assay revealed that CL90-Tw-SeNP2 showed remarkable antitumor effects against HepG2 cells, with an IC value of 49.13 μg/mL, by inducing cell apoptosis. Furthermore, an in vivo zebrafish assay to explore possible applications indicated that CL90-Tw-SeNP2 could inhibit the proliferation and migration of tumors and the zebrafish angiogenesis. These results indicated that CL90-Tw-SeNP2 could be a potential agent for cancer treatment, especially against human liver hepatoma cancer.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.142DOI Listing
July 2021

Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves.

Food Chem 2021 Dec 6;364:130310. Epub 2021 Jun 6.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields. However, a few studies were performed to explore polysaccharides in this plant. Herein, SRP70-1 was derived from S. rebaudiana. Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da. SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues. Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α. Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1. This study revealed that SRP70-1 may be useful for the development of functional foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.130310DOI Listing
December 2021

Occupational Physical Activity and New-Onset Hypertension: A Nationwide Cohort Study in China.

Hypertension 2021 Jul 1;78(1):220-229. Epub 2021 Jun 1.

Division of Nephrology, Nanfang Hospital, Southern Medical University, National Clinical Research Center for Kidney Disease, State Key Laboratory of Organ Failure Research, Guangdong Provincial Institute of Nephrology, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangzhou, China (Q.L., Y.Z., P.H., Z.Z., M.L., C.Z., H.L., C.L., X.Q.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.121.17281DOI Listing
July 2021

Rapid discrimination between tuberculosis and sarcoidosis using next-generation sequencing.

Int J Infect Dis 2021 Jul 15;108:129-136. Epub 2021 May 15.

Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address:

Objectives: Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis (MTB), has similar clinical, radiological, and histopathological characteristics to sarcoidosis (SA). Accurately distinguishing SA from TB remains a clinical challenge.

Methods: A total of 44 TB patients and 47 SA patients who were clinically diagnosed using chest radiography, pathological examination, routine smear microscopy, and microbial culture were enrolled in this study. The MTB genome was captured and sequenced directly from tissue specimens obtained upon operation or biopsy, and the feasibility of next-generation sequencing (NGS) for the MTB genome in the differential diagnosis of TB from SA was evaluated.

Results: Using a depth >10× and coverage >15% of the sequencing data, TB patients were identified via the NGS approach directly using operation or biopsy specimens without clinical pretreatment. The sensitivity, specificity, and concordance of the NGS method were 81.8% (36/44), 95.7% (45/47), and 89.0% (81/91), respectively (kappa = 0.78, 95% confidence interval 0.65-0.91; P<0.001).

Conclusions: This study established an improved NGS strategy for rapidly distinguishing patients with TB from those with SA and has potential clinical benefits.
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http://dx.doi.org/10.1016/j.ijid.2021.05.028DOI Listing
July 2021

Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.

Carbohydr Polym 2021 Aug 15;265:118080. Epub 2021 Apr 15.

State Key Laboratory of Medicinal Chemistry Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin, 300350, People's Republic of China. Electronic address:

A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata. The absolute molecular weight was determined as 1.26 × 10 g/mol. Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose. Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose. Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models. Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis. These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
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http://dx.doi.org/10.1016/j.carbpol.2021.118080DOI Listing
August 2021

Cytotoxic and Antiangiogenetic Xanthones Inhibiting Tumor Proliferation and Metastasis from .

J Nat Prod 2021 05 27;84(5):1515-1523. Epub 2021 Apr 27.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.

Eight prenylated xanthones including four new analogues were extracted and purified from the leaves of . Multiple techniques including UV, 1D and 2D NMR, and HRESIMS were used to determine the structures of the isolated xanthones. These xanthones were evaluated for their cytotoxicity toward human cancer cells, and compound exhibited activity against HeLa cells. A cytotoxic mechanism examination revealed the active compound induced cell apoptosis by arresting the cell cycle, increasing the levels of ROS, and inhibiting the expression of p-STAT3 in HeLa cells. In in vivo zebrafish experiments, compound was found to block tumor proliferation and migration and have antiangiogenetic activity, and thus seems worthy of further laboratory evaluation.
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http://dx.doi.org/10.1021/acs.jnatprod.0c01354DOI Listing
May 2021

The synthesis of greenish phosphorus on carbon substrates.

Chem Commun (Camb) 2021 Apr;57(33):3975-3978

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 457, P. R. China.

A phosphorus allotrope called greenish phosphorus was successfully synthesized via a simple chemical vapor deposition method. We revealed that the critical factors in the formation mechanism of greenish phosphorus are the partial pressure of the phosphorus vapor and the structure of the substrate. On the substrates of a glassy carbon wafer and carbon paper, the edge carbon structure can activate P4 molecules, allowing them to polymerize due to strong adsorption (Ead = -1.62 eV). Greenish phosphorus possesses a distinct crystal structure, different from red phosphorus and black phosphorus, thus leading to unique physical and chemical properties, and potential applications in optical, electrical, and magnetic fields.
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http://dx.doi.org/10.1039/d1cc01419fDOI Listing
April 2021

Structural characteristics and in vitro and in vivo immunoregulatory properties of a gluco-arabinan from Angelica dahurica.

Int J Biol Macromol 2021 Jul 16;183:90-100. Epub 2021 Apr 16.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

A water-soluble polysaccharide identified here as ADP80-2 was acquired from Angelica dahurica. ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol. The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue. In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage. In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2. These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents. Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.077DOI Listing
July 2021

Prescribed performance incremental adaptive optimal fault-tolerant control for nonlinear systems with actuator faults.

ISA Trans 2021 Mar 19. Epub 2021 Mar 19.

College of Automation Engineering, Nanjing University of Astronautics and Astronautics, Nanjing 211106, Jiangsu, China.

In this paper, a model-free incremental adaptive optimal fault-tolerant controller with prescribed performance is proposed for nonlinear systems subject to actuator faults. Considering the system actuator redundancy, an actuator grouping scheme is introduced according to the actuator functions. An incremental adaptive fault observer is designed to estimate the faults. The recursive least squares (RLS) identification is utilized to identify the incremental system parameters. Both dynamic process requirements and optimal performance index of the closed loop system are taken into consideration by combining prescribed performance bound (PPB) and incremental adaptive dynamic programming (IADP). Simulations are given to verify the effectiveness of the proposed IADP reconfigurable fault-tolerant control scheme.
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http://dx.doi.org/10.1016/j.isatra.2021.03.011DOI Listing
March 2021

RNAMotifContrast: a method to discover and visualize RNA structural motif subfamilies.

Nucleic Acids Res 2021 06;49(11):e61

Department of Computer Science, University of Central Florida, Orlando, FL 32816, USA.

Understanding the 3D structural properties of RNAs will play a critical role in identifying their functional characteristics and designing new RNAs for RNA-based therapeutics and nanotechnology. While several existing computational methods can help in the analysis of RNA properties by recognizing structural motifs, they do not provide the means to compare and contrast those motifs extensively. We have developed a new method, RNAMotifContrast, which focuses on analyzing the similarities and variations of RNA structural motif characteristics. In this method, a graph is formed to represent the similarities among motifs, and a new traversal algorithm is applied to generate visualizations of their structural properties. Analyzing the structural features among motifs, we have recognized and generalized the concept of motif subfamilies. To asses its effectiveness, we have applied RNAMotifContrast on a dataset of known RNA structural motif families. From the results, we observed that the derived subfamilies possess unique structural variations while holding standard features of the families. Overall, the visualization approach of this method presents a new perspective to observe the relation among motifs more closely, and the discovered subfamilies provide opportunities to achieve valuable insights into RNA's diverse roles.
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http://dx.doi.org/10.1093/nar/gkab131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216276PMC
June 2021

d-PBWT: dynamic positional Burrows-Wheeler transform.

Bioinformatics 2021 Feb 24. Epub 2021 Feb 24.

Department of Computer Science, University of Central Florida, Orlando, FL, USA.

Motivation: Durbin's positional Burrows-Wheeler transform (PBWT) is a scalable data structure for haplotype matching. It has been successfully applied to identical by descent (IBD) segment identification and genotype imputation. Once the PBWT of a haplotype panel is constructed, it supports efficient retrieval of all shared long segments among all individuals (long matches) and efficient query between an external haplotype and the panel. However, the standard PBWT is an array-based static data structure and does not support dynamic updates of the panel.

Results: Here, we generalize the static PBWT to a dynamic data structure, d-PBWT, where the reverse prefix sorting at each position is stored with linked lists.We also developed efficient algorithms for insertion and deletion of individual haplotypes. In addition, we verified that d-PBWT can support all algorithms of PBWT. In doing so, we systematically investigated variations of set maximal match and long match query algorithms: while they all have average case time complexity independent of database size, they have different worst case complexities and dependencies on additional data structures.

Availability: The benchmarking code is available at genome.ucf.edu/d-PBWT.

Supplementary Information: Supplementary Materials are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab117DOI Listing
February 2021

Repressing mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAs.

Elife 2021 Feb 18;10. Epub 2021 Feb 18.

Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.

The regulation of stem cell fate is poorly understood. Genetic studies in lead to the hypothesis that a conserved cytoplasmic double-negative feedback loop consisting of the RNA-binding protein Trim71 and the let-7 microRNA controls the pluripotency and differentiation of stem cells. Although let-7-microRNA-mediated inhibition of Trim71 promotes differentiation, whether and how Trim71 regulates pluripotency and inhibits the let-7 microRNA are still unknown. Here, we show that Trim71 represses mRNA translation in mouse embryonic stem cells. Blocking this repression leads to a specific post-transcriptional increase of mature let-7 microRNAs, resulting in let-7-dependent stemness defects and accelerated differentiation in the stem cells. These results not only support the Trim71-let-7-microRNA bi-stable switch model in controlling stem cell fate, but also reveal that repressing the conserved pro-differentiation let-7 microRNAs at the mature microRNA level by Ago2 availability is critical to maintaining pluripotency.
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http://dx.doi.org/10.7554/eLife.66288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906602PMC
February 2021

Personalized genealogical history of UK individuals inferred from biobank-scale IBD segments.

BMC Biol 2021 02 16;19(1):32. Epub 2021 Feb 16.

School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, 77030, USA.

Background: The genealogical histories of individuals within populations are of interest to studies aiming both to uncover detailed pedigree information and overall quantitative population demographic histories. However, the analysis of quantitative details of individual genealogical histories has faced challenges from incomplete available pedigree records and an absence of objective and quantitative details in pedigree information. Although complete pedigree information for most individuals is difficult to track beyond a few generations, it is possible to describe a person's genealogical history using their genetic relatives revealed by identity by descent (IBD) segments-long genomic segments shared by two individuals within a population, which are identical due to inheritance from common ancestors. When modern biobanks collect genotype information for a significant fraction of a population, dense genetic connections of a person can be traced using such IBD segments, offering opportunities to characterize individuals in the context of the underlying populations. Here, we conducted an individual-centric analysis of IBD segments among the UK Biobank participants that represent 0.7% of the UK population.

Results: We made a high-quality call set of IBD segments over 5 cM among all 500,000 UK Biobank participants. On average, one UK individual shares IBD segments with 14,000 UK Biobank participants, which we refer to as "relatives." Using these segments, approximately 80% of a person's genome can be imputed. We subsequently propose genealogical descriptors based on the genetic connections of relative cohorts of individuals sharing at least one IBD segment and show that such descriptors offer important information about one's genetic makeup, personal genealogical history, and social behavior. Through analysis of relative counts sharing segments at different lengths, we identified a group, potentially British Jews, who has a distinct pattern of familial expansion history. Finally, using the enrichment of relatives in one's neighborhood, we identified regional variations of personal preference favoring living closer to one's extended families.

Conclusions: Our analysis revealed genetic makeup, personal genealogical history, and social behaviors at the population scale, opening possibilities for further studies of individual's genetic connections in biobank data.
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http://dx.doi.org/10.1186/s12915-021-00964-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888130PMC
February 2021

Dietary Carbohydrate Intake and New-Onset Hypertension: A Nationwide Cohort Study in China.

Hypertension 2021 Aug 8;78(2):422-430. Epub 2021 Feb 8.

From the Division of Nephrology, Nanfang Hospital, Southern Medical University; National Clinical Research Center for Kidney Disease; State Key Laboratory of Organ Failure Research; Guangdong Provincial Institute of Nephrology, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, China (Q.L., C.L., Y.Z., P.H., Z.Z., M.L., C.Z., Z.Y., Q.W., H.L., X.Q.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.16751DOI Listing
August 2021

[Exploration of the correlation between allergic rhinitis and weather factors in Nanning].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 01;35(1):1-8

Department of Otorhinolaryngology Head and Neck,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning,530021,China.

To investigate the characteristics of allergic rhinitis(AR) in the Nanning area and its correlation with meteorological factors. The characteristics of 16 969 cases of AR in Nanning from May 2011 to June 2018 were analyzed. Meteorological parameters were collected from the official website of the Nanning Meteorological Bureau, including weather conditions, temperature, and wind direction. The relationship between the onset characteristics of AR patients and meteorological factors were also analyzed. Among the 16 969 patients with AR, the top three inhalation allergens were dust mite(56.0%), house dust mite(50.5%), and cockroach(37.4%). The skin index(SI) of dust mite, house dust mite, cockroach is correlated with sex. The SI of dust mite, house dust mite, mugwort, cockroach are correlated with age, and inhaled allergens are correlated with disease course(<0.05). However, those relationships were not significant(<0.2). The proportion of AR patients visiting the clinic in summer was the highest(3.9%) in all of the four seasons. Gender was found to be related to the grade-4 positive results of dust mite(=0.826, 95%: 0.724-0.942), the grade-4 house dust mite(=0.777, 95%: 0.677-0.891) and the grade-3 positive results of cockroach(=1.236, 95%: 1.060-1.443), respectively. The the daytime weather conditions were related to the grade-4 positive results of dust mite(=0.830, 95%: 0.728-0.947) and the grade 1 positive results of house dust mite(=0.803, 95%: 0.694-0.929), respectively. The highest temperature was related to the grade-4 positive result of dust mite(=1.032, 95%: 1.020-1.043), grade-1(=1.028, 95%: 1.001-1.056) and grade-4 (=1.047, 95%: 1.021-1.075) positive result of house dust mite, grade 1(=1.023, 95%: 1.001-1.047) and grade-4(=1.050, 95%: 1.008-1.094) positive result of cockroach, respectively. The minimum temperature was related to the positive results of house dust mites at all grades and the grade-1 positive results of cockroach(=0.947, 95%: 0.924-0.971), but not to dust mites. The daytime wind direction was related to the grade-3 positive results of cockroach(=1.437, 95%: 1.157-1.785). The number of AR patients in Nanning is highest in summer. The female is more likely to suffer from dust mite and house dust mite, while the male is more likely to suffer from cockroach. Meteorological factors are correlated with the incidence of AR.The highertemperature, the rain and the southerly wind could cause the occurrence or aggravation of AR.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.01.001DOI Listing
January 2021

Relation of BMI and waist circumference with the risk of new-onset hyperuricemia in hypertensive patients.

QJM 2021 Jan 24. Epub 2021 Jan 24.

Institute of Biomedicine, Anhui Medical University, Hefei, China.

Background: We aimed to evaluate the relationship of body mass index (BMI) and waist circumference (WC) with the risk of new-onset hyperuricemia, and examine possible effect modifies in general hypertensive patients.

Methods: A total of 10,611 hypertensive patients with normal uric acid (UA) concentrations (<357μmol/L) at baseline were included from the UA Sub-study of the China Stroke Primary Prevention Trial (CSPPT). The primary outcome was new-onset hyperuricemia, defined as a UA concentration ≥417μmol/L in men or ≥ 357μmol/L in women at the exit visit.

Results: During a median follow-up duration of 4.4 years, 1663 (15.7%) participants developed new-onset hyperuricemia. When analyzed separately, increased BMI (≥25 kg/m2, quartile 3-4; OR, 1.46; 95% CI: 1.29-1.65), or increased WC (≥85cm for females, quartile 3-4; OR, 1.24; 95% CI: 1.08-1.42; and ≥84cm for males, quartile 3-4; OR, 1.30; 95% CI: 1.01-1.67) were each significantly associated with higher risk of new-onset hyperuricemia. When WC was forced into the model with BMI simultaneously, its significant association with new-onset hyperuricemia disappeared in females (<85 versus ≥85cm; OR, 0.96, 95% CI: 0.81-1.13) or males (≥84 versus <84cm; OR, 1.13; 95%CI: 0.84-1.52); however, BMI was still significantly related with new-onset hyperuricemia (≥25 versus <25kg/m2; OR, 1.48; 95%CI: 1.27-1.73). Moreover, the positive BMI & new-onset hyperuricemia association was more pronounced in participants with higher time-averaged on-treatment SBP (median: <138.3 versus ≥138.3 mmHg; P-interaction = 0.041).

Conclusions: Higher BMI, but not WC, is significantly and independently associated with an increased risk of new-onset hyperuricemia among hypertensive patients.
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http://dx.doi.org/10.1093/qjmed/hcaa346DOI Listing
January 2021

Structural properties and in vitro and in vivo immunomodulatory activity of an arabinofuranan from the fruits of Akebia quinata.

Carbohydr Polym 2021 Mar 17;256:117521. Epub 2020 Dec 17.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

In our continuous searching for natural active polysaccharides with immunomodulatory activity, an arabinofuranan (AQP70-3) was isolated and purified from the fruits of Akebia quinata (Houtt.) Decne. by using ion-exchange chromatography and gel permeation chromatography for the first time. AQP70-3 contained both α-l-Araf and β-l-Araf, and the absolute molecular weight was 1.06 × 10 g/mol. The backbone of AQP70-3 comprised →5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→, and →2,5)-α-l-Araf-(1→, with branches of →1)-β-l-Arafand →3)-α-l-Araf-(1→ residues. Biological assay suggested that AQP70-3 can stimulate phagocytic activity and promote the levels of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of RAW264.7 cells. Furthermore, AQP70-3 was found to increase the production of reactive oxygen species (ROS) and NO in zebrafish embryo model.
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http://dx.doi.org/10.1016/j.carbpol.2020.117521DOI Listing
March 2021

RAFFI: Accurate and fast familial relationship inference in large scale biobank studies using RaPID.

PLoS Genet 2021 01 21;17(1):e1009315. Epub 2021 Jan 21.

School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, Texas, United States of America.

Inference of relationships from whole-genome genetic data of a cohort is a crucial prerequisite for genome-wide association studies. Typically, relationships are inferred by computing the kinship coefficients (ϕ) and the genome-wide probability of zero IBD sharing (π0) among all pairs of individuals. Current leading methods are based on pairwise comparisons, which may not scale up to very large cohorts (e.g., sample size >1 million). Here, we propose an efficient relationship inference method, RAFFI. RAFFI leverages the efficient RaPID method to call IBD segments first, then estimate the ϕ and π0 from detected IBD segments. This inference is achieved by a data-driven approach that adjusts the estimation based on phasing quality and genotyping quality. Using simulations, we showed that RAFFI is robust against phasing/genotyping errors, admix events, and varying marker densities, and achieves higher accuracy compared to KING, the current leading method, especially for more distant relatives. When applied to the phased UK Biobank data with ~500K individuals, RAFFI is approximately 18 times faster than KING. We expect RAFFI will offer fast and accurate relatedness inference for even larger cohorts.
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http://dx.doi.org/10.1371/journal.pgen.1009315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853505PMC
January 2021

A Covalent P-C Bond Stabilizes Red Phosphorus in an Engineered Carbon Host for High-Performance Lithium-Ion Battery Anodes.

ACS Nano 2021 Feb 20;15(2):3365-3375. Epub 2021 Jan 20.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.

The red phosphorus (RP) anode has attracted great attention due to its high theoretical specific capacity (2596 mAh/g) and suitable lithiation potential. To solve the inherent poor electrical conductivity and the large volume expansion due to the lithiation process, a vaporization-condensation strategy is considered as a promising method. However, there are two important issues that deserve attention in the vaporization-condensation process. First, the low P mass loading in the carbon-based frameworks (∼30 wt %) limits the energy density. Second, a residual white phosphorus (WP) leads to the safety problems of flammability and high toxicity. Herein, we found that the edge structure of carbon framework can offer the strong adsorption for P and form a P-C bond, which accelerate the adsorption and polymerization of P leading to high P mass loading and safety. When the porous carbon (PC) with plenty of edge carbons was used as the matrix to load P by vaporization-condensation, the RP loading is close to the highest theoretical mass loading of ∼50 wt % calculated based on the feeding ratio of RP/PC = 1/1. Therefore, the RP-PC anode provides a high specific capacity of 965.2 mAh/g even after 1100 cycles at 1000 mA/g (equivalent to 1 C) and a high-rate capacity of 496.8 mAh/g at 8320 mA/g (equivalent to 16.7 C) after 1000 cycles (the specific capacity and current density are calculated based on the total weight of RP and PC).
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http://dx.doi.org/10.1021/acsnano.0c10370DOI Listing
February 2021

Structural elucidation of an immunological arabinan from the rhizomes of Ligusticum chuanxiong, a traditional Chinese medicine.

Int J Biol Macromol 2021 Feb 11;170:42-52. Epub 2020 Dec 11.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

In the present study, an immunological arabinan, LCP70-2A, was isolated from Ligusticum chuanxiong for the first time. The absolute molecular weight of LCP70-2A was determined to be 6.46 × 10 g/mol using the HPSEC-MALLS-RID method. The absolute configuration of arabinose in LCP70-2A was determined to be L-configuration. Physicochemical characterization revealed that LCP70-2A was a homogeneous polysaccharide and had a backbone of (1 → 5)-linked α-L-Araf with terminal α-L-arabinose residues at position O-2 and O-3. Molecular conformation analysis showed that LCP70-2A was a branching polysaccharide with a compact coil chain conformation in 0.1 M NaCl solution. In addition, in vitro cell assays showed that LCP70-2A can activate macrophages by enhancing the phagocytosis and potentiating the secretion of immunoregulatory factors including NO, TNF-α, IL-6, and IL-1β. Furthermore, LCP70-2A was proved to promote the production of ROS and NO using the zebrafish model, suggesting that LCP70-2A can be further developed as a candidate supplement for immunological enhancement.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.12.069DOI Listing
February 2021

Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription.

Genome Biol 2020 12 8;21(1):296. Epub 2020 Dec 8.

School of Life Science and Technology, ShanghaiTech University,, Beijing, China.

Introduction: Despite the long-observed correlation between H3K9me3, chromatin architecture, and transcriptional repression, how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear.

Result: Here, we develop EpiGo (Epigenetic perturbation induced Genome organization)-KRAB to introduce H3K9me3 at hundreds of loci spanning megabases on human chromosome 19 and simultaneously track genome organization. EpiGo-KRAB is sufficient to induce genomic clustering and de novo heterochromatin-like domain formation, which requires SETDB1, a methyltransferase of H3K9me3. Unexpectedly, EpiGo-KRAB-induced heterochromatin-like domain does not result in widespread gene repression except a small set of genes with concurrent loss of H3K4me3 and H3K27ac. Ectopic H3K9me3 appears to spread in inactive regions but is largely restricted from transcriptional initiation sites in active regions. Finally, Hi-C analysis showed that EpiGo-KRAB reshapes existing compartments mainly at compartment boundaries.

Conclusions: These results reveal the role of H3K9me3 in genome organization could be partially separated from its function in gene repression.
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http://dx.doi.org/10.1186/s13059-020-02201-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722448PMC
December 2020

Discovery of runs-of-homozygosity diplotype clusters and their associations with diseases in UK Biobank.

medRxiv 2020 Oct 27. Epub 2020 Oct 27.

Runs of homozygosity (ROH) segments, contiguous homozygous regions in a genome were traditionally linked to families and inbred populations. However, a growing literature suggests that ROHs are ubiquitous in outbred populations. Still, most existing genetic studies of ROH in populations are limited to aggregated ROH content across the genome, which does not offer the resolution for mapping causal loci. This limitation is mainly due to a lack of methods for efficient identification of shared ROH diplotypes. Here, we present a new method, ROH-DICE, to find large ROH diplotype clusters, sufficiently long ROHs shared by a sufficient number of individuals, in large cohorts. ROH-DICE identified over 1 million ROH diplotypes that span over 100 SNPs and shared by more than 100 UK Biobank participants. Moreover, we found significant associations of clustered ROH diplotypes across the genome with various self-reported diseases, with the strongest associations found between the extended HLA region and autoimmune disorders. We found an association between a diplotype covering the HFE gene and haemochromatosis, even though the well-known causal SNP was not directly genotyped nor imputed. Using genome-wide scan, we identified a putative association between carriers of an ROH diplotype in chromosome 4 and an increase of mortality among COVID-19 patients. In summary, our ROH-DICE method, by calling out large ROH diplotypes in a large outbred population, enables further population genetics into the demographic history of large populations. More importantly, our method enables a new genome-wide mapping approach for finding disease-causing loci with multi-marker recessive effects at population scale.
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http://dx.doi.org/10.1101/2020.10.26.20220004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605569PMC
October 2020

Microstructure of the hyoid bone based on micro-computed tomography findings.

Medicine (Baltimore) 2020 Oct;99(44):e22246

Human Anatomy Teaching and Research Section (Digital Medical Center), Inner Mongolia Medical University Basic Medical College.

In this study, micro-CT was used to observe the microscopic anatomy of the hyoid bone, examine the variation of the trabecular bone inside the hyoid bone, and investigate the internal structure of the hyoid bone.A total of 22 hyoid bones were scanned using micro-CT. The changes in the internal bone trabeculae were assessed with 3D reconstructions, and the fine anatomical structure of the hyoid bone was further analyzed.Micro-CT images showed the microstructure of various parts of the hyoid bone. There were significant differences in total volume, bone volume, bone area, bone density, and volume fraction between the body and greater horns of the hyoid bone (P < .05), but no significant differences in the ratio of bone area/volume and bone surface density were found between the body and greater horns of the hyoid bone (P > .05). In addition, significant differences in the trabecular bone measurements, bone trabecular connectivity, and Euler number were found between the body and greater horns of the hyoid bone (P < .05). Other parameters, including bone trabecular thickness, number of trabecular bones, bone trabecular structure model index, and anisotropy of bone trabeculae, did not differ between the body and greater horns of the hyoid bone (P > .05). There was noticeably ossified healing at the joint between the body and greater horns of the hyoid bone.Micro-CT can adequately display the internal structure of the hyoid bone. The identified bone structure may help clarify the physiological function of the hyoid bone. The present findings provide a theoretical basis for further studies aimed at pathological changes due to hyoid injury in clinical and forensic medicine.
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http://dx.doi.org/10.1097/MD.0000000000022246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598853PMC
October 2020

The Evaluation of the Multiple Myeloma Pathological Osseous Tissue of Microarchitecture.

Curr Med Imaging 2021 ;17(4):513-516

Human Anatomy Teaching and Research Section (Digital Medical Center), Inner Mongolia Medical University Basic Medical College, Hohhot, 010059, China.

Background: The typical imaging finding of MM is bone destruction in the form of diffuse patchy osteolytic lesions or punctate destruction. However, it is difficult to accurately determine the fine structure of bone affected by MM with these techniques due to low specificity and sensitivity.

Introduction: This study aimed to investigate the microscopic anatomical morphology and analyze the microstructure changes of trabeculae affected by multiple myeloma (MM) based on micro-CT.

Methods: MM-affected and normal trabecular bone samples were imaged by micro-CT to obtain bone structure parameters to assess statistical differences between them and evaluate the degree of microstructural damage of MM-affected trabeculae.

Results: Micro CT images clearly showed the microstructure of MM-affected trabeculae. The degree of trabecular osteoporosis varied with the severity of MM. There were significant differences in the structural parameters between MM-affected and normal trabeculae (P < 0.05).

Conclusion: Micro-CT clearly reveals the microstructure of MM-affected trabeculae. The obtained bone structure data will help to determine the degree of bone damage caused by MM and assess the efficacy.
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http://dx.doi.org/10.2174/1573405616666201015144015DOI Listing
January 2021

Isolation, structural elucidation, and immunoregulation properties of an arabinofuranan from the rinds of Garcinia mangostana.

Carbohydr Polym 2020 Oct 11;246:116567. Epub 2020 Jun 11.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

In our search for bioactive polysaccharides as immunomodulatory agents, an arabinofuranan (GMP90-1) was purified and characterized from the rinds of Garcinia mangostana L. GMP90-1 (absolute molecular weight: 5.30 × 10 g/mol) was found to be composed of arabinose, galactose, and rhamnose. The backbone of GMP90-1 was determined as (1→5)-linked α-l-Araf, (1→2,3,5)-linked α-l-Araf, (1→3,5)-linked α-l-Araf, (1→6)-linked β-d-Galp, and (1→2)-linked α-l-Rhap. Conformational analysis revealed GMP90-1 to exist as a rigid rod structure in sodium chloride solution. To explore its potential as immunomodulatory agents, an in vitro cell screening was performed and GMP90-1 was found to significantly enhance the phagocytic uptake of neutral red and improve the secreted level of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of macrophages. Furthermore, the cellular immunomodulatory activities were confirmed by the in vivo zebrafish experiment, which suggested that GMP90-1 with immunomodulatory effects could be considered as a potential immunomodulatory for immune diseases.
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http://dx.doi.org/10.1016/j.carbpol.2020.116567DOI Listing
October 2020

A heteropolysaccharide purified from leaves of Ilex latifolia displaying immunomodulatory activity in vitro and in vivo.

Carbohydr Polym 2020 Oct 26;245:116469. Epub 2020 May 26.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:

A novel polysaccharide (ILP50-2) was extracted, isolated and purified from the leaves of Ilex latifolia Thunb. Its structure was characterized as a repeating unit consisting of α-L-Araf-(1→, →3)-α-L-Araf-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, β-D-Galp-(1→, →4)-β-D-Galp-(1→, →4)-β-D-Glcp-(1→, →6)-α-D-Manp-(1→, and →3,6)-α-D-Galp-(1→. The absolute molecular weight of ILP50-2 was 1.49 × 10 g/mol, which adapted a compact coil conformation in 0.1 M NaCl solution with R of 25.4 nm. Furthermore, ILP50-2 exhibited immunoregulatory activity, mainly through enhancing the phagocytosis ability of macrophages and prompting the release of nitric oxide (NO) and cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Simultaneously, ILP50-2 was found to significantly increase the release of ROS and NO in zebrafish embryos, showing immunoregulatory effects in vivo.
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http://dx.doi.org/10.1016/j.carbpol.2020.116469DOI Listing
October 2020

Bioassay-guided isolation and evaluation of anti-osteoporotic polysaccharides from Morinda officinalis.

J Ethnopharmacol 2020 Oct 12;261:113113. Epub 2020 Jul 12.

Center for Clinical Precision Medication, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510006, China; School of Clinical Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China. Electronic address:

Ethnopharmacological Relevance: Morinda officinalis is a well-known Chinese tonic herb that has shown clinical efficacy in the treatment of bone disease. However, its anti-osteoporotic potential and the M. officinalis polysaccharides (MOPs) responsible for activity require further investigation.

Aim Of The Study: This study aimed to investigate the anti-osteoporotic effects of different MOP fractions in ovariectomized (OVX) rats, and to identify the osteoprotective components by bioassay-guided isolation.

Materials And Methods: MOPs were prepared by hot water and alkali extraction, separated into three fractions (MO50, MO70, and MOB) and evaluated in the classic OVX rat model and in MC3T3-E1 cells for anti-osteoporotic activity.

Results: Administration of MOPs (400 mg/kg/day) provided significant protection against ovariectomy-induced bone loss and biomechanical dysfunction in rats. Treated animals exhibited reduced deterioration of trabecular microarchitecture and lower levels of bone turnover markers. Bioactivity-guided fractionation led to the isolation of two inulin-type fructans from MO50, MOW50-1 and MOP50-2, with potential anti-osteoporotic activities. These consisted of (2 → 1)-linked β-D-fructosyl residues with degrees of polymerization (DP) of 7 and 13, respectively. Furthermore, MOW50-1 promoted osteogenic differentiation of MC3T3-E1 cells by increasing alkaline phosphatase activity.

Conclusions: These data suggest very strongly that MOPs, especially MO50 and MOW50-1, may play important roles in the prevention and treatment of osteoporosis.
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http://dx.doi.org/10.1016/j.jep.2020.113113DOI Listing
October 2020

Dataset on the effect of sodium sources on the morphology, crystallite size and carbon content of NaTi(po)/c composite prepared by an in situ process.

Data Brief 2020 Aug 18;31:105871. Epub 2020 Jun 18.

School of Chemical and Environmental Engineering, Jiangsu University of Technology, Changzhou, 213001, China.

The data presented in this manuscript showed the effects of the sodium sources on the morphology, crystallite size and carbon content of NaTi(PO)/C composite obtained after heat treatment under N atmosphere of the as-spraydried powders. The morphology, crystalline size and carbon content of three products with different sodium sources were investigated. The data are related to "Synthesis of NaTi(PO)@C microspheres by an in situ process and their electrochemical properties" (Mao et al.,2020).
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http://dx.doi.org/10.1016/j.dib.2020.105871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334292PMC
August 2020
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