Publications by authors named "Shao-Hua Wang"

151 Publications

and studies of bis (indol-3-yl) methane derivatives as potential α-glucosidase and α-amylase inhibitors.

J Enzyme Inhib Med Chem 2021 Dec;36(1):1938-1951

School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, PR China.

In this paper, bis (indol-3-yl) methanes (BIMs) were synthesised and evaluated for their inhibitory activity against α-glucosidase and α-amylase. All synthesised compounds showed potential α-glucosidase and α-amylase inhibitory activities. Compounds (IC: 7.54 ± 1.10 μM), (IC: 9.00 ± 0.97 μM), and (IC: 9.57 ± 0.62 μM) presented strongest inhibitory activities against α-glucosidase, that were ∼ 30 times stronger than acarbose. Compounds (IC: 32.18 ± 1.66 µM), (IC: 31.47 ± 1.42 µM), and (IC: 30.91 ± 0.86 µM) showed strongest inhibitory activities towards α-amylase, ∼ 2.5 times stronger than acarbose. The mechanisms and docking simulation of the compounds were also studied. Compounds and exhibited bifunctional inhibitory activity against these two enzymes. Furthermore, compounds showed no toxicity against 3T3-L1 cells and HepG2 cells.HighlightsA series of bis (indol-3-yl) methanes (BIMs) were synthesised and evaluated inhibitory activities against -glucosidase and α-amylase.Compound exhibited promising activity (IC = 7.54 ± 1.10 μM) against -glucosidase.Compound exhibited promising activity (IC = 30.91 ± 0.86 μM) against α-amylase.In silico studies were performed to confirm the binding interactions of synthetic compounds with the enzyme active site.
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http://dx.doi.org/10.1080/14756366.2021.1971976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409970PMC
December 2021

Effect of molecular mass and sulfate content of fucoidan from Sargassum siliquosum on antioxidant, anti-lipogenesis, and anti-inflammatory activity.

J Biosci Bioeng 2021 Aug 10. Epub 2021 Aug 10.

Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Chemical and Materials Engineering, College of Engineering, Tunghai University, Taichung, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung, Taiwan. Electronic address:

Macroalgae (seaweeds) are abundant in functional polysaccharides known for their unique biochemical activities. In this study, the antioxidant, anti-lipogenic, and anti-inflammatory activities of the fucoidan extracted from brown seaweed Sargassum siliquosum were investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging ability, lipid synthesis inhibition, and suppression of pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) production, respectively. To examine the effect of molecular mass on fucoidan's bioactivities above, the extracted fucoidan was subject to hydrogen peroxide-mediated partial hydrolysis to obtain lower molecular mass compounds within the range of 107.3-3.2 kDa. Results indicated that fucoidan's antioxidant activity increased with a corresponding decrease in molecular mass; the dosage for the half-maximal response (EC) dropped from 2.58 to 1.82 mg/mL when the molecular mass decreased from 107.3 to 3.2 kDa. In addition, both the anti-lipogenesis and anti-inflammatory activities of fucoidan were significantly enhanced by 71.1% and 36.7%, respectively, when the molecular mass decreased to about 3 kDa. To further test the effect of sulfation on fucoidan's bioactivities, low molecular mass fucoidan was treated with SO-DMF to increase the sulfate content. The results indicated that when sulfate content increased from 18.7% to 32.1%, EC of DPPH decreased from 1.82 mg/mL to 0.86 mg/mL and the anti-inflammatory activity also increased by 35.2%; however, the anti-lipogenesis activity decreased.
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http://dx.doi.org/10.1016/j.jbiosc.2021.06.005DOI Listing
August 2021

Recent development and applications of semipinacol rearrangement reactions.

Chem Sci 2021 Jul 28;12(27):9262-9274. Epub 2021 Jun 28.

State Key Laboratory of Applied Organic Chemistry and School of Pharmacy, Lanzhou University Lanzhou 730000 P. R. China.

As has been well-recognized, semipinacol rearrangement functions as an exceptionally useful methodology in the synthesis of β-functionalized ketones, creation of quaternary carbon centers, and construction of challenging carbocycles. Due to their versatile utilities in organic synthesis, development of novel rearrangement reactions has been a vibrant topic that continues to shape the research field. Recent breakthroughs in novel electrophiles, tandem processes, and enantioselective catalytic transformations further enrich the toolbox of this chemistry and spur the strategic applications of this methodology in natural product synthesis. These achievements will be discussed in this minireview.
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http://dx.doi.org/10.1039/d1sc02386aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314203PMC
July 2021

LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity.

Cell Death Dis 2021 05 28;12(6):557. Epub 2021 May 28.

Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, Guangdong, China.

Maintaining proper mitochondrial respiratory function is crucial for alleviating cardiac metabolic disorders during obesity, and mitophagy is critically involved in this process. Long non-coding RNA H19 (H19) is crucial for metabolic regulation, but its roles in cardiac disorders, mitochondrial respiratory function, and mitophagy during obesity are largely unknown. In this study, palmitic acid (PA)-treated H9c2 cell and Lep mice were used to investigate cardiac metabolic disorders in vitro and in vivo, respectively. The effects of H19 on metabolic disorders, mitochondrial respiratory function, and mitophagy were investigated. Moreover, the regulatory mechanisms of PA, H19, mitophagy, and respiratory function were examined. The models tested displayed a reduction in H19 expression, respiratory function and mitochondrial number and volume, while the expression of mitophagy- and Pink1/Parkin signaling-related proteins was upregulated, as indicated using quantitative real-time PCR, Seahorse mitochondrial stress test analyzer, transmission electron microscopy, fluorescence indicators and western blotting. Forced expression of H19 helped to the recoveries of respiratory capacity and mitochondrial number while inhibited the levels of mitophagy- and Pink1/Parkin signaling-related proteins. Pink1 knockdown also attenuated PA-induced mitophagy and increased respiratory capacity. Mechanistically, RNA pull-down, mass spectrometry, and RNA-binding protein immunoprecipitation assays showed that H19 could hinder the binding of eukaryotic translation initiation factor 4A, isoform 2 (eIF4A2) with Pink1 mRNA, thus inhibiting the translation of Pink1 and attenuation of mitophagy. PA significantly increased the methylation levels of the H19 promoter region by upregulation Dnmt3b methylase levels, thereby inhibiting H19 transcription. Collectively, these findings suggest that DNA methylation-mediated the downregulation of H19 expression plays a crucial role in cardiomyocyte or H9c2 cells metabolic disorders and induces cardiac respiratory dysfunction by promoting mitophagy. H19 inhibits excessive mitophagy by limiting Pink1 mRNA translation, thus alleviating this cardiac defect that occurs during obesity.
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http://dx.doi.org/10.1038/s41419-021-03821-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163878PMC
May 2021

Undiagnosed pleural effusion treated with traditional Chinese medicine: A case report.

Explore (NY) 2021 Feb 16. Epub 2021 Feb 16.

Department of Respiratory Medicine, Dongzhimen Hospital affiliated to Beijing University of Chinese Medicine (BUCM), No. 5 Haiyuncang, Dongcheng District, Beijing 100700, China. Electronic address:

The main clinical manifestations of pleural effusion are exertional dyspnea, predominantly dry cough, and pleuritic chest pain. To treat pleural effusion appropriately, it is important to determine its etiology; which however, remains unclear in nearly 20% of cases.A 73-year-old man with a history of invasive pulmonary tuberculosis (TB), had been experiencing chest congestion and dyspnea with undiagnosed pleural effusion for six years. After a series of clinical examination and laboratory tests, there was still no clear diagnosis. Despite administering diuretics and intermittent draining, the patient's condition aggravated progressively. He sought further treatment at Dongzhimen Hospital Respiratory Outpatient Clinic. The patient was treated with Zanthoxylum and Trichosanthes Decoction (Jiao Mu Gua Lou Tang). After one and a half years, his symptoms greatly improved and ultrasound revealed that the pleural effusion had apparently absorbed.It is suggested that TCM herbal formulas can play a critical role in preventing the progression of complicated, undiagnosed pleural effusion, especially in cases of poor response to conventional therapy and thoracentesis. Additional studies on the functions and mechanisms of the medicinals are warranted.
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http://dx.doi.org/10.1016/j.explore.2021.02.005DOI Listing
February 2021

Characterization of the complete chloroplast genome of 'Yunning No.1' lemon ().

Mitochondrial DNA B Resour 2021 Feb 9;6(2):425-427. Epub 2021 Feb 9.

Yunnan Academy of Agricultural Sciences, Institute of Tropical and Subtropical Cash Crops, Baoshan, PR China.

'Yunning No.1' lemon, a mutant of Eureka lemon, is originally found in Yunnan province of China and is the main cultivated lemon variety there. In this study, we assembled and annotated its chloroplast genome using Illumina Hiseq-2500 whole genome re-sequencing data. Its chloroplast genome is 160,141 bp in size, containing a 87,754 bp large single copy region, a 18,385 bp small single copy region and a pair of 27,001 bp inverted repeat region. Like many citrus species, 114 unique genes (including 80 protein-coding genes, 30 tRNAs and 4 rRNAs) could be identified from the chloroplast genome of 'Yunning No.1'. Phylogenetic analysis revealed that the 'Yunning No.1' chloroplast genome was closest to .
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http://dx.doi.org/10.1080/23802359.2020.1870889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889090PMC
February 2021

Construction and Analysis of a ceRNA Network in Cardiac Fibroblast During Fibrosis Based on and Data.

Front Genet 2020 21;11:503256. Epub 2021 Jan 21.

Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Aims: Activation of cardiac fibroblasts (CF) is crucial to cardiac fibrosis. We constructed a cardiac fibroblast-related competing endogenous RNA (ceRNA) network. Potential functions related to fibrosis of "hub genes" in this ceRNA network were explored.

Materials And Methods: The Gene Expression Omnibus database was searched for eligible datasets. Differentially expressed messenger (m)RNA (DE-mRNA) and long non-coding (lnc)RNA (DE-lncRNA) were identified. microRNA was predicted and validated. A predicted ceRNA network was constructed and visualized by Cytoscape, and ceRNA crosstalk was validated. A Single Gene Set Enrichment Analysis (SGSEA) was done, and the Comparative Toxicogenomics Database (CTD) was employed to analyze the most closely associated pathways and diseases of DE-mRNA in the ceRNA network. The functions of DE-mRNA and DE-lncRNA in the ceRNA network were validated by small interfering (si)RNA depletion.

Results: The GSE97358 and GSE116250 datasets (which described differentially expressed genes in human cardiac fibroblasts and failing ventricles, respectively) were used for analyses. Four-hundred-and-twenty DE-mRNA and 39 DE-lncRNA, and 369 DE-mRNA and 93 DE-lncRNA were identified, respectively, in the GSE97358 and GSE116250 datasets. Most of the genes were related to signal transduction, cytokine activity, and cell proliferation. Thirteen DE-mRNA with the same expression tendency were overlapped in the two datasets. Twenty-three candidate microRNAs were predicted and the expression of 11 were different. Only two DE-lncRNA were paired to any one of 11 microRNA. Finally, two mRNA [ADAM metallopeptidase domain 19, () and transforming growth factor beta induced, ()], three microRNA (, , and ) and two lncRNA ( and ) constituted our ceRNA network. siRNA against increased and expression, and decreased and expression, whereas siRNA against increased and decreased expression. and were closely related to the TGF-β1 pathway and cardiac fibrosis, as shown by SGSEA and CTD, respectively. Depletion of two mRNA or two lncRNA could alleviate CF activation.

Conclusions: The CF-specific ceRNA network, including two lncRNA, three miRNA, and two mRNA, played a crucial role during cardiac fibrosis, which provided potential target genes in this field.
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http://dx.doi.org/10.3389/fgene.2020.503256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859616PMC
January 2021

The Application of Fine Needle Aspiration Biopsy in the Diagnosis of Axillary Masses.

Acta Cytol 2021 3;65(3):213-219. Epub 2021 Feb 3.

Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, China,

Introduction: We intend to determine the diagnostic power of fine needle aspiration biopsy (FNAB) for differentiation between malignant and benign lesions on axillary masses and draw the physicians' attention to the benefits of FNAB cytology in the diagnosis of axillary masses.

Methods: In this study, 1,328 patients with an axillary mass diagnosed by FNAB were retrospectively reviewed. These cases were registered at the affiliated hospital of Southwest Medical University (China), July 2014 to June 2017. Cytological results were verified either by histopathology following surgical resection or clinical follow-up.

Results: Of the 1,328 patients affected by axillary masses, 987 (74.3%) cases were female, and 341 (25.7%) cases were male. The highest incidence of patients was in the age group of 41-50 years (375, 28.2%). There were 1,129 (85.0%) patients with benign lesions and 199 (15.0%) with malignant lesions. Of the 199 malignant lesions cases, 21 cases were lymphomas, 2 cases were accessory breast cancers, and 176 cases were lymph node metastatic tumors. Under lymph node metastases, the most frequent primary tumors were breast cancer (141, 80.1%), followed by lung cancer (21, 11.9%). According to the study, the characters of 1,328 cases showed statistically significant difference (χ2 = 4.534, p = 0.033), and the incidence of females with axillary mass was significantly higher than that of males. There was a statistically significant difference in the distribution of benign and malignant cases in the patient age groups (χ2 = 1.129, p = 0.000), and the incidence of patients of 41-50 years of age was significantly higher than that of other patients. The diagnostic accuracy of FNAB in axillary masses was analyzed with the results of 95.98% of sensitivity, 99.56% of specificity, 97.45% of positive predictive value, and 99.29% of negative predictive value.

Conclusion: Our results confirm that FNAB is a valuable initial screening method regarding pathologic diagnosis of axillary mass, in particular with respect to malignancy in 41- to 50-year-old female patients.
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http://dx.doi.org/10.1159/000513149DOI Listing
June 2021

Characterization of the complete chloroplast genome of Yuanjiang wild Ichang papeda () in China.

Mitochondrial DNA B Resour 2020 Sep 16;5(3):3349-3350. Epub 2020 Sep 16.

Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences, Baoshan, P. R. China.

Ichang papeda () is an endemic perennial plant in China. In this study, we assembled and annotated the complete chloroplast genome of Yuanjiang wild Ichang papeda using Illumina Hiseq-2500 sequencing data. The chloroplast genome is constituted of 160,996 bp, containing a 87,634 bp large single-copy region, a 18,762 bp small single-copy region, and a pair of 27,300 bp inverted repeat regions. The chloroplast genome contains 114 unique genes, including 80 protein-coding genes, 30 tRNAs and 4 rRNAs. Phylogenetic analysis showed that the relationship between the chloroplast gennomes of and . is the closest, which consistently support their chloroplast relationships.
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http://dx.doi.org/10.1080/23802359.2020.1820397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781987PMC
September 2020

The Structure, Function, and Mechanisms of Action of Enterovirus Non-structural Protein 2C.

Front Microbiol 2020 14;11:615965. Epub 2020 Dec 14.

Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China.

Enteroviruses are a group of RNA viruses belonging to the family . They include human enterovirus groups A, B, C, and D as well as non-human enteroviruses. Enterovirus infections can lead to hand, foot, and mouth disease and herpangina, whose clinical manifestations are often mild, although some strains can result in severe neurological complications such as encephalitis, myocarditis, meningitis, and poliomyelitis. To date, research on enterovirus non-structural proteins has mainly focused on the 2A and 3C proteases and 3D polymerase. However, another non-structural protein, 2C, is the most highly conserved protein, and plays a vital role in the enterovirus life cycle. There are relatively few studies on this protein. Previous studies have demonstrated that enterovirus 2C is involved in virus uncoating, host cell membrane rearrangements, RNA replication, encapsidation, morphogenesis, ATPase, helicase, and chaperoning activities. Despite ongoing research, little is known about the pathogenesis of enterovirus 2C proteins in viral replication or in the host innate immune system. In this review, we discuss and summarize the current understanding of the structure, function, and mechanism of the enterovirus 2C proteins, focusing on the key mutations and motifs involved in viral infection, replication, and immune regulation. We also focus on recent progress in research into the role of 2C proteins in regulating the pattern recognition receptors and type I interferon signaling pathway to facilitate viral replication. Given these functions and mechanisms, the potential application of the 2C proteins as a target for anti-viral drug development is also discussed. Future studies will focus on the determination of more crystal structures of enterovirus 2C proteins, which might provide more potential targets for anti-viral drug development against enterovirus infections.
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http://dx.doi.org/10.3389/fmicb.2020.615965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767853PMC
December 2020

Structure and Biological Activity Analysis of Fucoidan Isolated from .

ACS Omega 2020 Dec 12;5(50):32447-32455. Epub 2020 Dec 12.

Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.

Fucoidans are heterologous polysaccharides commonly seen in brown macroalgae and are known for their biological activity including anticancer, antiangiogenic, immunomodulation, and antiviral properties. The brown macroalga was used for the extraction and analysis of fucoidan in this study. The fucoidan was indicated as a galactofucoidan composed of sugars, uronate, and sulfate at a ratio of 12:1:4 and its purity was 85% based on the abovementioned three major components. Structural analysis by electrospray ionization collision-induced dissociation tandem mass spectroscopy revealed that the purified fucoidan consisted of a carbohydrate chain composed of (1→3)-linked or (1→4)-linked l-fucose residues, with sulfate groups at C-2 and C-4 positions. Galactose residues with (1→4)-linkages function as the branch points and they are located at the C-3 or C-4 position of fucose residues. Galactose residues are sulfated mainly at C-4 and C-6, while some sulfation can also be seen at C-2. The fucoidan purified from demonstrated antioxidant, anti-inflammatory, and antilipogenic activities.
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http://dx.doi.org/10.1021/acsomega.0c04591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758953PMC
December 2020

Interleukin-33 alleviates diabetic cardiomyopathy through regulation of endoplasmic reticulum stress and autophagy via insulin-like growth factor-binding protein 3.

J Cell Physiol 2021 Jun 13;236(6):4403-4419. Epub 2020 Nov 13.

Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

Prolonged endoplasmic reticulum (ER) stress is the key driving force behind diabetic cardiomyopathy (DCM). Autophagy is extensively implicated in adaptive mechanisms for cell survival. Interleukin-33 (IL-33) is known to be a potent cardiac protector, but its roles in DCM, ER stress, and autophagy are currently unknown. We aimed to explore the effects of IL-33 on DCM and characterize the roles that ER stress and autophagy play in DCM. The effects of IL-33 on DCM, ER stress, and autophagy were characterized both in db/db mice and in palmitic acid (PA)-treated cardiomyocytes. The manipulators of ER stress and autophagy were used to clarify their roles in DCM remittance conferred by IL-33. Gene expression analysis was used to identify IL-33-dependent regulators of ER stress and autophagy. Both db/db mice and PA-treated cells presented with enhanced levels of ER stress, apoptosis, and lipid deposition, as well as impaired autophagy, all of which could be reversed by IL-33. Treatment with IL-33 improved the cardiac diastolic function of diabetic mice. Nonselective autophagy inhibitors, such as 3-methyladenine (3-MA) or wortmannin, abolished the protective effects of IL-33, resulting in an increase in both ER stress and apoptosis. Strikingly, insulin-like growth factor-binding protein 3 (IGFBP3) was identified as the gene most significantly differentially expressed between IL-33 and control groups. Knockdown of IGFBP3 expression, similar to the effect of nonselective autophagy inhibitors, resulted in high levels of ER stress, impaired autophagy, and apoptosis that were not rescued upon treatment with IL-33. IL-33 abates DCM by alleviating ER stress and promoting autophagy. IGFBP3 is essential for IL-33-induced ER stress resolution and autophagic enhancement during DCM.
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http://dx.doi.org/10.1002/jcp.30158DOI Listing
June 2021

microRNA regulation of pluripotent state transition.

Essays Biochem 2020 12;64(6):947-954

Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, China.

microRNAs (miRNAs) play essential roles in mouse embryonic stem cells (ESCs) and early embryo development. The exact mechanism by which miRNAs regulate cell fate transition during embryo development is still not clear. Recent studies have identified and captured various pluripotent stem cells (PSCs) that share similar characteristics with cells from different stages of pre- and post-implantation embryos. These PSCs provide valuable models to understand miRNA functions in early mammalian development. In this short review, we will summarize recent work towards understanding the function and mechanism of miRNAs in regulating the transition or conversion between different pluripotent states. In addition, we will highlight unresolved questions and key future directions related to miRNAs in pluripotent state transition. Studies in these areas will further our understanding of miRNA functions in early embryo development, and may lead to practical means to control human PSCs for clinical applications in regenerative medicine.
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http://dx.doi.org/10.1042/EBC20200028DOI Listing
December 2020

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Cell Prolif 2020 Nov 29;53(11):e12914. Epub 2020 Sep 29.

Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, China.

Objectives: Mouse embryonic stem cell (mESC) culture contains various heterogeneous populations, which serve as excellent models to study gene regulation in early embryo development. The heterogeneity is typically defined by transcriptional activities, for example, the expression of Nanog or Rex1 mRNA. Our objectives were to identify mESC heterogeneity that are caused by mechanisms other than transcriptional control.

Materials And Methods: Klf3 mRNA and protein were analysed by RT-qPCR, Western blotting or immunofluorescence in mESCs, C2C12 cells, early mouse embryos and various mouse tissues. An ESC reporter line expressing KLF3-GFP fusion protein was made to study heterogeneity of KLF3 protein expression in ESCs. GFP-positive mESCs were sorted for further analysis including RT-qPCR and RNA-seq.

Results: In the majority of mESCs, KLF3 protein is actively degraded due to its proline-rich sequence and highly disordered structure. Interestingly, KLF3 protein is stabilized in a small subset of mESCs. Transcriptome analysis indicates that KLF3-positive mESCs upregulate genes that are initially activated in 8-cell embryos. Consistently, KLF3 protein but not mRNA is dramatically increased in 8-cell embryos. Forced expression of KLF3 protein in mESCs promotes the expression of 8-cell-embryo activated genes.

Conclusions: Our study identifies previously unrecognized heterogeneity due to KLF3 protein expression in mESCs.
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http://dx.doi.org/10.1111/cpr.12914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653263PMC
November 2020

Au(I)-Catalyzed Cyclization/Semipinacol Rearrangement Reaction of Allenes to Construct Quaternary Carbon-Containing Scaffolds.

Org Lett 2020 09 4;22(18):7073-7077. Epub 2020 Sep 4.

School of Pharmacy & State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P.R. China.

The development of methods toward the construction of quaternary carbon centers has been a hot topic in recent years. In this work, an Au(I)-catalyzed intramolecular cyclization/semipinacol rearrangement of allene-containing allylic silyl ether was developed to provide a direct strategy for the construction of multisubstituted cyclohexene-type compounds with a quaternary carbon center in moderate to good yields. In particular, this method provides an alternative synthetic strategy for the construction of a multisubstituted spirocyclo[4.5]decane skeleton and may be applied to the synthesis of related bioactive molecules and their derivatives, thus facilitating the corresponding functional studies.
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http://dx.doi.org/10.1021/acs.orglett.0c02262DOI Listing
September 2020

Long non-coding RNA Linc00092 inhibits cardiac fibroblast activation by altering glycolysis in an ERK-dependent manner.

Cell Signal 2020 10 9;74:109708. Epub 2020 Jul 9.

Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, PR China; Laboratory of Cardiac Electrophysiology and Arrhythmia in Guangdong Province, Guangzhou, Guangdong 510120, PR China. Electronic address:

Aims: Cardiac fibroblast (CF) activation is the key event for cardiac fibrosis. The role of glycolysis and the glycolysis-related lncRNAs in CF activation are unknown. Thus, we aimed to investigate the role of glycolysis in CF activation and to identify the glycolysis-related lncRNAs involved.

Main Methods: Glycolysis-related lncRNAs were searched and their expression profiles were validated in activated human CF (HCF) and human failing heart tissues. Expression of the target lncRNA was manipulated to determine its effects on HCF activation and glycolysis. The underlying mechanisms of lncRNA-dependent glycolysis regulation were also addressed.

Key Findings: HCF activation induced by transforming growth factor-β1 was accompanied by an enhanced glycolysis, and 2-Deoxy-d-glucose, a specific glycolysis inhibitor, dramatically attenuated HCF activation. Twenty-eight glycolysis-related lncRNAs were identified and Linc00092 expression was changed mostly upon HCF activation. In human heart tissue, Linc00092 is primarily expressed in cardiac fibroblasts. Linc00092 knockdown activated HCFs with enhanced glycolysis, while its overexpression rescued the activated phenotype of HCFs and down-regulated glycolysis. Restoration of glycolysis abolished the anti-fibrotic effects conferred by Linc00092. Linc00092 inhibited ERK activation in activated HCFs, and ERK inhibition counteracted the fibrotic phenotype in Linc00092 knockdown HCFs.

Significance: These results revealed that Linc00092 could attenuate HCF activation by suppressing glycolysis. The inhibition of ERK by Linc00092 may play an important role in this process. Together, this provides a better understanding of the mechanism of CF activation and may serve as a novel target for cardiac fibrosis treatment.
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http://dx.doi.org/10.1016/j.cellsig.2020.109708DOI Listing
October 2020

The diagnosis of primary thyroid lymphoma by fine-needle aspiration, cell block, and immunohistochemistry technique.

Diagn Cytopathol 2020 Nov 1;48(11):1041-1047. Epub 2020 Jul 1.

Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Aim: Primary thyroid lymphoma (PTL) is a rare malignant disease. Its prognosis depends on early diagnosis. The role of fine-needle aspiration (FNA), including smear cytology, cell block (CB) techniques, and immunohistochemistry (IHC) sections in the diagnosis of PTL is still unclear. Here we reported 19 cases of PTL and literature review to evaluate the diagnostic accuracy for lymphoma by cytology.

Methods: Our study retrospectively reviewed 19 patients diagnosed with PTL at the affiliated hospital of Southwest Medical University in China from June 2011 to May 2019. According to the Bethesda system for reporting thyroid cytopathology, the CB sections were evaluated for the presence of single tumor cells. IHC was performed on CB.

Results: The diagnostic accuracy for PTL of FNA, CB with smears, and the joint application of the three methods (FNA + CB + IHC) of our study with 19 cases was 68.4% (13/19), 83.3% (15/18), and 100% (17/17), respectively.

Conclusion: The present study demonstrates that FNA has low sensitivity in diagnosing PTL, but the joint application of FNA, CB, and IHC might provide high diagnostic accuracy for lymphoma and should be applied in all cases where the clinical suspicion is high regardless of the FNA findings.
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http://dx.doi.org/10.1002/dc.24526DOI Listing
November 2020

Direct synthesis of 2-substituted benzonitriles via alkylcyanation of arynes with N,N-disubstituted aminomalononitriles.

Chem Commun (Camb) 2020 Jul 10;56(55):7641-7644. Epub 2020 Jun 10.

School of Pharmacy & State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P. R. China.

An efficient alkylcyanation of in situ generated arynes by N,N-disubstituted aminomalononitriles is described, enabling the direct synthesis of 2-substituted benzonitriles.
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http://dx.doi.org/10.1039/d0cc01591aDOI Listing
July 2020

Patterns of Extrathoracic Metastases in Different Histological Types of Lung Cancer.

Front Oncol 2020 19;10:715. Epub 2020 May 19.

Department of Thoracic Surgery, Huashan Hospital, Fudan University, Shanghai, China.

Lung cancer is the leading cause of cancer-related deaths mainly attributable to metastasis, especially extrathoracic metastasis. This large-cohort research is aimed to explore metastatic profiles in different histological types of lung cancer, as well as to assess clinicopathological and survival significance of diverse metastatic lesions. Lung cancer cases were extracted and enrolled from the Surveillance, Epidemiology, and End Results (SEER) database. χ-tests were conducted to make comparisons of metastatic distribution among different histological types and odds ratios were calculated to analyze co-occurrence relationships between different metastatic lesions. Kaplan-Meier methods were performed to analyze survival outcomes according to different metastatic sites and Cox regression models were conducted to identify independent prognostic factors. In total, we included 159,241 lung cancer cases with detailed metastatic status and complete follow-up information. In order to understand their metastatic patterns, we elucidated the following points in this research: (1) Comparing the frequencies of different metastatic lesions in different histological types. The frequency of bone metastasis was highest in adenocarcinoma, squamous cell carcinoma, LCLC and NSCLC/NOS, while liver was the most common metastatic site in SCLC. (2) Elaborating the tendency of combined metastases. Bi-site metastases occurred more common than tri-site and tetra-site metastases. And several metastatic sites, such as bone and liver, intended to co-metastasize preferentially. (3) Clarifying the prognostic significance of single-site and bi-site metastases. All single-site metastases were independent prognostic factors and co-metastases ended up with even worse survival outcomes. Thus, our findings would be beneficial for research design and clinical practice.
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http://dx.doi.org/10.3389/fonc.2020.00715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248315PMC
May 2020

Electrosprayed chitosan/alginate/polyvinyl alcohol nanoparticles as boric acid carriers for Boron neutron capture therapy.

Nanomedicine (Lond) 2020 05 24;15(11):1067-1077. Epub 2020 Apr 24.

Department of Engineering & System Science, Frontier Research Center on Fundamental & Applied Sciences of Matters, National Tsing Hua University, Hsinchu, 30013, Taiwan.

To improve the killing efficacy of head and neck squamous cells (SAS) by boric acid-mediated boron neutron capture therapy (BNCT).  Boric acid-containing chitosan/alginate/polyvinyl alcohol nanoparticles (B-capNPs) were manufactured using the nano-electrospray process.  Less than 10% of the boric acid leaked from the B-capNPs over 2 days. The B-capNPs killed up to 2.8-fold more SAS cells and reduced cytotoxicity tenfold when compared with pure boric acid alone. B-capNPs show selective uptake in tumor cells with tumor/normal ratios of SAS to normal (NIH 3T3) and macrophage (RAW 264.7) cells of 4.0 and 3.5, respectively, which are greater than the minimum acceptable tumor/normal ratio for BNCT of 2.5. These findings illustrate that B-capNPs may be more superior as BNCT drugs than pure boric acid.
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http://dx.doi.org/10.2217/nnm-2019-0465DOI Listing
May 2020

Facile access to diverse all-carbon quaternary center containing spirobicycles by exploring a tandem Castro-Stephens coupling/acyloxy shift/cyclization/semipinacol rearrangement sequence.

Chem Sci 2020 Mar 16;11(15):3878-3884. Epub 2020 Mar 16.

State Key Laboratory of Applied Organic Chemistry, School of Pharmacy, Lanzhou University Lanzhou 730000 P. R. China

Efficient combination of two or more reactions into a practically useful purification free sequence is of great significance for the achievement of structural complexity and diversity, and an important approach for the development of new synthetic strategies that are industrially step-economic and environmentally friendly. In this work, a facile and efficient method for the construction of highly functionalized spirocyclo[4.5]decane derivatives containing a synthetically challenging quaternary carbon center has been successfully developed through the realization of a tandem Castro-Stephens coupling/1,3-acyloxy shift/cyclization/semipinacol rearrangement sequence. Thus a series of multi-substituted spirocyclo[4.5]decane and functionalized cyclohexane skeletons with a phenyl-substituted quaternary carbon center have been constructed using this method as illustrated by 24 examples in moderate to good yields. The major advantages of this method over the known strategies are better transformation efficiency (four consecutive transformations in one tandem reaction), product complexity and diversity. As a support of its potential application, a quick construction of the key tetracyclic diterpene skeleton of waihoensene has been achieved.
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http://dx.doi.org/10.1039/d0sc00102cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152660PMC
March 2020

Enantioselective Catalytic Aldehyde α-Alkylation/Semipinacol Rearrangement: Construction of α-Quaternary-δ-Carbonyl Cycloketones and Total Synthesis of (+)-Cerapicol.

Angew Chem Int Ed Engl 2020 05 18;59(22):8471-8475. Epub 2020 Mar 18.

School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.

An enantioselective aldehyde α-alkylation/semipinacol rearrangement was achieved through organo-SOMO catalysis. The catalytically generated enamine radical cation serves as a carbon radical electrophile that can stereoselectively add to the alkene of an allylic alcohol and initiate ensuing ring-expansion of cyclopropanol or cyclobutanol. This tandem reaction enables the production of a wide range of nonracemic functionalizable α-quaternary-δ-carbonyl cycloketones in high yields and excellent enantioselectivity from simple aldehydes and allylic alcohols. As a key step, the intramolecular reaction was also successfully applied in the asymmetric total synthesis of (+)-cerapicol.
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http://dx.doi.org/10.1002/anie.202001100DOI Listing
May 2020

Knockdown of Long Noncoding RNAs of Maternally Expressed 3 Alleviates Hyperoxia-Induced Lung Injury via Inhibiting Thioredoxin-Interacting Protein-Mediated Pyroptosis by Binding to miR-18a.

Am J Pathol 2020 05 19;190(5):994-1005. Epub 2020 Feb 19.

Neonatal Intensive Care Unit, Women and Children Health Institute Futian, University of South China, Shenzhen, P.R. China. Electronic address:

Long-term hyperoxia exposure may cause lung damage with characteristic inflammation. Long noncoding RNA of maternally expressed 3 (MEG3) is up-regulated in lung tissues exposed to hyperoxia; however, the underlying mechanism is unclear. Hyperoxia-induced cells and mouse models were used to study these mechanisms. Molecular assays were used to detect cell viability, cytotoxicity, and expression of miR-18a, MEG3, and inflammatory cytokines. The interaction among MEG3, miR-18a, and thioredoxin-interacting protein (TXNIP) was verified; and pyroptosis-related proteins were analyzed. The in vivo model was established by exposing MEG3 knockdown mice to hyperoxia. Hematoxylin and eosin staining was used to assess pathologic alterations of lung tissues. Hyperoxia suppressed cell viability, induced cell damage, and exacerbated the secretion of IL-1β and IL-18. Hyperoxia inhibited miR-18a, with increased expression of MEG3, TXNIP, and nonobese diabetic-like receptor family pyrin domain containing 3 (NLRP3). MEG3 aggravated TXNIP expression by binding to miR-18a. Knockdown of MEG3 rescued hyperoxia-induced pyroptosis by up-regulating miR-18a. Furthermore, knockdown of MEG3 inhibited NLRP3 inflammasome activity and caspase-1 signaling by miR-18a. In vivo knockdown of MEG3 and overexpression of miR-18a relieved hyperoxia-induced lung injury via restraining NLRP3 inflammasome-mediated pyroptosis, whereas miR-18a inhibition reversed these effects. In conclusion, knockdown of MEG3 inhibits pyroptosis to alleviate hyperoxia lung injury by suppressing NLRP3 inflammasome and caspase-1 signaling via regulating miR-18a-TXNIP axis.
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http://dx.doi.org/10.1016/j.ajpath.2019.12.013DOI Listing
May 2020

Synthesis and biological evaluation of coumarin derivatives as α-glucosidase inhibitors.

Eur J Med Chem 2020 Mar 30;189:112013. Epub 2019 Dec 30.

School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, PR China; International Healthcare Innovation Institute (Jiangmen), Jiangmen, 529020, PR China; School of Pharmacy & State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000, PR China. Electronic address:

In this study, two series of coumarin derivatives 5a∼i and 6a∼i were synthesized, and their inhibitory activity against α-glucosidase was determined. The results indicated that most of the synthesized derivatives exhibited prominent inhibitory activities against α-glucosidase. Among them, compounds 5a and 5b showed the strongest inhibition with the IC values of 19.64 μM and 12.98 μM, respectively. Enzyme kinetic studies of compounds 5a and 5b proved that their inhibition was reversible and a mixed type. The K and K values of compound 5a were calculated to be 27.39 μM and 13.02 μM, respectively, and the corresponding values for compound 5b being 27.02 μM and 13.65 μM, respectively. The docking studies showed that compound 5b could be inserted into the active pocket of α-glucosidase and form hydrogen bonds with LYS293 to enhance the binding affinity.
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http://dx.doi.org/10.1016/j.ejmech.2019.112013DOI Listing
March 2020

[Risk factors for cow's milk protein allergy in infants: a multicenter survey].

Zhongguo Dang Dai Er Ke Za Zhi 2020 Jan;22(1):42-46

Department of Pediatrics, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518017, China.

Objective: To investigate the risk factors for cow's milk protein allergy (CMPA) among infants through a multicenter clinical study.

Methods: A total of 1 829 infants, aged 1-12 months, who attended the outpatient service of the pediatric department in six hospitals in Shenzhen, China from June 2016 to May 2017 were enrolled as subjects. A questionnaire survey was performed to screen out suspected cases of CMPA. Food avoidance and oral food challenge tests were used to make a confirmed diagnosis of CMPA CMPA. A multivariate logistic regression analysis was used to investigate the risk factors for CMPA.

Results: Among the 1 829 infants, 82 (4.48%) were diagnosed with CMPA. The multivariate logistic regression analysis showed that maternal food allergy (OR=4.91, 95%CI: 2.24-10.76, P<0.05), antibiotic exposure during pregnancy (OR=3.18, 95%CI: 1.32-7.65, P<0.05), and the introduction of complementary food at an age of <4 months (OR=3.55, 95%CI: 1.52-8.27, P<0.05) were risk factors for CMPA, while exclusive breastfeeding (OR=0.21, 95%CI: 0.08-0.58, P<0.05) and the introduction of complementary food at an age of >6 months (OR=0.38, 95%CI: 0.17-0.86, P<0.05) were protective factors.

Conclusions: The introduction of complementary food at an age of <4 months, maternal food allergy, and antibiotic exposure during pregnancy are risk factors for CMPA in infants.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7389720PMC
January 2020

[Effect of Two Soil Synergists on Ammonia Volatilization in Paddy Fields].

Huan Jing Ke Xue 2019 Aug;40(8):3746-3752

Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.

Nitrification inhibitor and biochar are commonly used as soil synergists. Among them, nitrification inhibitor can increase crop yields and N use efficiency, while biochar is a relatively new way of using biomass resources and has certain adsorption characteristics. In order to reduce nitrogen loss and environmental pollution caused by ammonia volatilization in paddy fields, a pot experiment with chemical fertilizer application (CN) as a control was conducted to study the effects of biochar (B), nitrapyrin (CP), and compound application (BCP) on pH, NH-N concentration dynamics in the flood water, rice yields, and ammonia volatilization from paddy fields. The results showed that the application of these two synergists had no significant effect on rice yields, and the nitrification inhibitors had a tendency to increase rice yields. The two synergists significantly increased ammonia volatilization from paddy fields, accounting for 25%-35% of the total N rate. Ammonia volatilization during periods of fertilizer application accounted for 86%-91% of the total loss, representing the main period of ammonia volatilization. Compared with the CN treatment, the CP treatment increased NH-N concentrations in flood water and the loss of ammonia via volatilization, which was increased by 59.18% and mainly occurred during a week after the basal fertilization(138%) and spike fertilization (48%), and non-fertilization stage (78%). Biochar had a promoting effect on ammonia volatilization with typically phased characteristics. The initial increasing effect of biochar on ammonia volatilization was higher than during the later stages, when NH-N concentrations and the pH of flood water showed the same trend. In addition, the coupling of nitrification inhibitor and biochar significantly increased the total loss of ammonia via volatilization loss due to the promotion effect of CP and B. The problem of increased ammonia volatilization loss caused by the application of nitrification inhibitors requires further research.
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http://dx.doi.org/10.13227/j.hjkx.201901189DOI Listing
August 2019

MiR-199a-5p represses the stemness of cutaneous squamous cell carcinoma stem cells by targeting Sirt1 and CD44ICD cleavage signaling.

Cell Cycle 2020 01 6;19(1):1-14. Epub 2019 Dec 6.

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, P.R. China.

Tumorigenic cancer stem cells (CSCs) exist in various tumors including the cutaneous squamous cell carcinoma (cSCC) as a minor subpopulation and are tightly associated with metastasis and therapeutic resistance. Better understanding of CSCs properties is essential for the novel therapeutic strategy targeted toward these cancers. The cSCC stem cells (cSCCSCs) were enriched from a cSCC cell line A431 by repeated sphere culture, and identified via the expression analysis of stemness marker genes and CD44 proteolysis. MiR-199a-5p was previously reported to be related with the proteolysis modulation of CD44, so the specific regulation mechanisms were verified by overexpression and . MiR-199a-5p is under-expressed in cSCCSCs and functions as a tumor suppressive molecule. Overexpression of miR-199a-5p reduced the stemness of cSCCSCs and inhibited cell proliferation. By targeting the deacetylase Sirt1, miR-199a-5p inhibited cellular proteolysis of CD44 and reduced the CD44 intracellular domain (CD44ICD) release and nuclear translocation. Overexpression of CD44ICD reversed the effects of miR-199a-5p overexpression or Sirt1 silencing, and increased the transcriptional expression of stemness genes. Our results revealed that the miR-199a-5p/Sirt1/CD44ICD signaling pathway regulates cSCCSCs progression by affecting its migration ability and tumorigenicity, therefore can be utilized to develop a curative approach for cSCC.: CSCs: cancer stem cells; cSCC cutaneous squamous cell carcinoma; cSCCSCs: cSCC stem cells; CD44ICD: CD44 intracellular domain; HA: hyaluronic acid; HNSCC: hand and neck squamous cell carcinoma; ESCC: esophageal squamous cell carcinoma;MMPs: matrix metalloproteinases; SFM: sphere formation medium; EGF: epidermal growth factor; bFGF: basic fibroblast growth factor; BSA: bovine serum albumin; CCK-8: cell counting kit-8.
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http://dx.doi.org/10.1080/15384101.2019.1689482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927716PMC
January 2020

Roles of the mammalian target of rapamycin (mTOR) signaling pathway in the repair of hyperoxia-induced acute lung injury.

Adv Clin Exp Med 2020 Jan;29(1):13-23

Neonatal Intensive Care Unit, Women and Children Health Institute Futian, University of South China, Shenzhen, China.

Background: Rapamycin inhibits the mammalian target of rapamycin (mTOR) activity and has been proven effective for the treatment of lung injury.

Objectives: The objective of this study was to investigate the roles of the mTOR pathway and its inhibitor rapamycin in the repair of hyperoxia-induced acute lung injury (ALI).

Material And Methods: Firstly, premature rat lung fibroblast L929 cells were cultured under different oxygen concentrations (40%, 60%, and 90%). At day 3, 7 and 14 after exposure, MTT assay and flow cytometry were used to evaluate the effect of oxygen stress on cell viability and apoptosis of L929 cells, respectively. Secondly, microscopy, MTT assay and flow cytometry was used to investigate the effect of 10 nM rapamycin on 90% O2 exposed L929 cells. We also used small interfering RNAs (siRNAs) to abrogate the expression of mTOR in 90% O2 exposed L929 cells, and then evaluated the apoptosis and cell viability using flow cytometry and the MTT assay, respectively. In addition, western blot was used to detect the protein expression of Bcl-2, p53, TGF-β and connective tissue growth factor (CTGF). A hyperoxia-induced lung injury model was established in Sprague Dawley (SD) rats in order to evaluate the histopathological changes in lung tissues and expression of the mTOR pathway and fibrosis related factors.

Results: Exposure to 40%, 60% or 90% oxygen all significantly inhibited the growth of L929 cells. Application of 10 nM rapamycin was found to effectively promote apoptosis of 90% O2 exposed L929 cells. In addition, mTOR siRNA promoted the apoptosis and inhibited the growth of L929 cells. Rapamycin inhibited the activation of the mTOR signaling pathway, down-regulated the expression of downstream proteins p70S6K and 4EBP1, reduced the collagen deposition and the production of fibrosis-inducing factors, including TGF-β and CTGF in hyperoxia-induced lung injury rats.

Conclusions: Rapamycin may be useful for the treatment of hyperoxia-induced acute lung injury (ALI) by inhibiting the activation of mTOR signaling pathway.
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http://dx.doi.org/10.17219/acem/76170DOI Listing
January 2020

Catalytic Asymmetric Total Syntheses of (-)-Galanthamine and (-)-Lycoramine.

J Org Chem 2019 10 18;84(19):12664-12671. Epub 2019 Sep 18.

State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering , Lanzhou University , Lanzhou 730000 , P. R. China.

The catalytic asymmetric total syntheses of the biologically important and therapeutically valuable alkaloids (-)-galanthamine and (-)-lycoramine have been divergently achieved from commercially available 3-butyn-1-ol. A newly developed spirocyclic pyrrolidine (SPD)-catalyzed enantioselective Robinson annulation rapidly constructs the key -hydrodibenzofuran core, which bears an all-carbon quaternary stereocenter of the target molecules with an excellent stereoselective control. Additionally, the current asymmetric synthetic strategy provides an alternative approach toward the syntheses of (-)-galanthamine and its analogues.
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http://dx.doi.org/10.1021/acs.joc.9b01971DOI Listing
October 2019

[Hematoma Expansion within 24 hours of Hypertensive Intracerebral Hemorrhage and Its Association with Signs on Nonenhanced Computed Tomography].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2019 Aug;41(4):472-478

Department of Radiology,Nanshan People's Hospital,Shenzhen,Guangdong 518052,China.

To explore the correlation between hematoma expansion within 24 hours of hypertensive intracerebral hemorrhage and signs on nonenhanced computed tomography(NECT). Methods The clinical data and CT images of 185 patients with hypertensive intracerebral hemorrhage were retrospectively analyzed.The differences in CT parameters were compared between the expansion group and the unexpanded group.Binary logistic regression analysis was performed on the indicators with statistical significance between the two groups to identify the potential correlation between CT parameters and hematoma expansion.The roles of blend sign,lobulation sign,and black hole sign in predicting early hematoma expansion were assessed. Results The CT quantitative data including initial volume,maximum diameter,minimum diameter,maximum CT value,mean CT value,difference between maximum diameter and minimum diameter showed no significant difference between these two group(all >0.05).CT qualitative data including blend sign,lobulation sign,and black hole sign were significantly different(<0.05)but the differences became not significant after the hematoma broke into the ventricles.Binary logistic regression analysis showed that blend sign,lobulation sign,and black hole sign were independent risk factors for early hematoma expansion,with sensitivity,specificity,positive predictive value,and negative predictive value of the combined signs and the early hematoma expansion calculated by the four-fold table method being 78.4%,59.0%,42.1%,and 87.8%,respectively,and the Youden index was 0.374.Its Youden index was closer to 1 than the blend sign,the black hole sign,and the lobulation sign. Conclusion The blend sign,lobulation sign,and black hole sign in NECT can be used to predict hematoma expansion within 24 hours after hypertensive cerebral hemorrhage.
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http://dx.doi.org/10.3881/j.issn.1000-503X.11256DOI Listing
August 2019
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